AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09233


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09233.t1 MTR_8g097140 84.746 236 32 2 1 233 1 235 2.58e-134 377
MS.gene09233.t1 MTR_1g016270 47.909 263 85 8 3 218 4 261 1.97e-57 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAAAATTGGAGAAAATTT+TGG 0.213621 4.1:-14895048 None:intergenic
AAATTCAATCAAGAAGTTAA+TGG 0.280474 4.1:+14895281 MS.gene09233:CDS
GGAGGCTATGATGTTATTCT+TGG 0.292173 4.1:+14895092 MS.gene09233:CDS
TCTGCTGACAATGGTGCTTC+AGG 0.313597 4.1:+14895071 MS.gene09233:CDS
AAAGAGTTTCGCAATGTTGA+TGG 0.379797 4.1:+14895236 MS.gene09233:CDS
GATTCACAACGACCGGTAAT+CGG 0.388566 4.1:-14894996 None:intergenic
CGAAATTCGATGCCGATTAC+CGG 0.390204 4.1:+14894984 None:intergenic
TGGAGGGCCGATGGGTCCTC+GGG 0.410669 4.1:+14895529 MS.gene09233:CDS
GTTTAAGTTCCTTGTGAAGC+TGG 0.424424 4.1:-14895145 None:intergenic
GTCCTCGGGACATAGAAATT+AGG 0.434748 4.1:+14895543 MS.gene09233:CDS
GTGGAGGGCCGATGGGTCCT+CGG 0.438544 4.1:+14895528 MS.gene09233:CDS
AACTTTATGCTGCGGAGAAA+AGG 0.440522 4.1:+14895600 MS.gene09233:CDS
ATTGTCAGCAGAGACAAAAT+TGG 0.442188 4.1:-14895060 None:intergenic
TTAAACTATCAAAATCTCAT+AGG 0.445350 4.1:+14895162 MS.gene09233:CDS
GAGAGAATTGAAAGTATGTC+GGG 0.447028 4.1:+14895641 MS.gene09233:CDS
CGGAGGGTTAGTTGCTCCTC+GGG 0.449386 4.1:+14895361 MS.gene09233:CDS
TATTCTTGGAAAGACTTTCA+AGG 0.486432 4.1:+14895106 MS.gene09233:CDS
AGAGAGAATTGAAAGTATGT+CGG 0.498267 4.1:+14895640 MS.gene09233:CDS
ACAAACAAGAAACTTGAAGT+AGG 0.507231 4.1:+14895566 MS.gene09233:CDS
TTTAGATGATTCACAACGAC+CGG 0.509528 4.1:-14895003 None:intergenic
TGCGAAGAGTGGAGGGCCGA+TGG 0.514612 4.1:+14895520 MS.gene09233:CDS
AAGAGTTTCGCAATGTTGAT+GGG 0.523006 4.1:+14895237 MS.gene09233:CDS
ATTCTTGGAAAGACTTTCAA+GGG 0.523914 4.1:+14895107 MS.gene09233:CDS
GGGACAAGAACAACAGAAAG+AGG 0.527695 4.1:+14895475 MS.gene09233:CDS
GCGAAGAGTGGAGGGCCGAT+GGG 0.536215 4.1:+14895521 MS.gene09233:CDS
CTCCTCGGGATGTAGAAGTC+AGG 0.538589 4.1:+14895375 MS.gene09233:CDS
TTTAGAAGTTGAAAATGCGA+AGG 0.547721 4.1:+14895454 MS.gene09233:CDS
GCGGAGGGTTAGTTGCTCCT+CGG 0.558909 4.1:+14895360 MS.gene09233:CDS
CAAACAAGAAACTTGAAGTA+GGG 0.559654 4.1:+14895567 MS.gene09233:CDS
AGCAATGATGCGAAGAGTGG+AGG 0.563241 4.1:+14895512 MS.gene09233:CDS
GCAATGATGCGAAGAGTGGA+GGG 0.571141 4.1:+14895513 MS.gene09233:CDS
GAATGAAGCCGTGAGTGAGA+AGG 0.573910 4.1:+14895409 MS.gene09233:CDS
TAAGAAGAAAATGAAACACT+AGG 0.578476 4.1:+14895673 MS.gene09233:CDS
TGAGAAGGAGAAAATGCAAA+GGG 0.591298 4.1:+14895424 MS.gene09233:CDS
AAGAAGAAAATGAAACACTA+GGG 0.592449 4.1:+14895674 MS.gene09233:CDS
GTCCTAATTTCTATGTCCCG+AGG 0.595550 4.1:-14895545 None:intergenic
TTCTAAGCAACAGAAAGAGA+TGG 0.598515 4.1:+14895304 MS.gene09233:CDS
CTAAGCAATGATGCGAAGAG+TGG 0.602938 4.1:+14895509 MS.gene09233:CDS
CTTGCTCAACCAGCTTCACA+AGG 0.605167 4.1:+14895136 MS.gene09233:CDS
AAAGATAAGTAACAATGCGA+AGG 0.605305 4.1:+14895331 MS.gene09233:CDS
TCTATGTCCCGAGGACCCAT+CGG 0.610923 4.1:-14895536 None:intergenic
TTAGAAGTTGAAAATGCGAA+GGG 0.610992 4.1:+14895455 MS.gene09233:CDS
GCTGACAATGGTGCTTCAGG+AGG 0.622331 4.1:+14895074 MS.gene09233:CDS
GAGAAAAGGAAGAATCAAGA+TGG 0.623121 4.1:+14895614 MS.gene09233:CDS
GACCTGACTTCTACATCCCG+AGG 0.639086 4.1:-14895377 None:intergenic
GTGAGAAGGAGAAAATGCAA+AGG 0.641271 4.1:+14895423 MS.gene09233:CDS
AATGCGAAGGTTGAAAGCGG+AGG 0.641854 4.1:+14895344 MS.gene09233:CDS
ATGCGAAGGTTGAAAGCGGA+GGG 0.642455 4.1:+14895345 MS.gene09233:CDS
TGAGAGTAAACTTTATGCTG+CGG 0.645952 4.1:+14895592 MS.gene09233:CDS
AACAATGCGAAGGTTGAAAG+CGG 0.662379 4.1:+14895341 MS.gene09233:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCAATCAAGAAGTTAA+TGG + chr4.1:14895281-14895300 MS.gene09233:CDS 20.0%
!! TTAAACTATCAAAATCTCAT+AGG + chr4.1:14895162-14895181 MS.gene09233:CDS 20.0%
!! GACAAAATTGGAGAAAATTT+TGG - chr4.1:14895051-14895070 None:intergenic 25.0%
AAAGATAAGTAACAATGCGA+AGG + chr4.1:14895331-14895350 MS.gene09233:CDS 30.0%
ACAAACAAGAAACTTGAAGT+AGG + chr4.1:14895566-14895585 MS.gene09233:CDS 30.0%
ATTCTTGGAAAGACTTTCAA+GGG + chr4.1:14895107-14895126 MS.gene09233:CDS 30.0%
CAAACAAGAAACTTGAAGTA+GGG + chr4.1:14895567-14895586 MS.gene09233:CDS 30.0%
TATTCTTGGAAAGACTTTCA+AGG + chr4.1:14895106-14895125 MS.gene09233:CDS 30.0%
TTAGAAGTTGAAAATGCGAA+GGG + chr4.1:14895455-14895474 MS.gene09233:CDS 30.0%
TTTAGAAGTTGAAAATGCGA+AGG + chr4.1:14895454-14895473 MS.gene09233:CDS 30.0%
! AGAGAGAATTGAAAGTATGT+CGG + chr4.1:14895640-14895659 MS.gene09233:CDS 30.0%
AAAGAGTTTCGCAATGTTGA+TGG + chr4.1:14895236-14895255 MS.gene09233:CDS 35.0%
AAGAGTTTCGCAATGTTGAT+GGG + chr4.1:14895237-14895256 MS.gene09233:CDS 35.0%
ATTGTCAGCAGAGACAAAAT+TGG - chr4.1:14895063-14895082 None:intergenic 35.0%
GAGAAAAGGAAGAATCAAGA+TGG + chr4.1:14895614-14895633 MS.gene09233:CDS 35.0%
TAGAAGTCAGGTCAAAAAAG+AGG + chr4.1:14895387-14895406 MS.gene09233:CDS 35.0%
TGAGAAGGAGAAAATGCAAA+GGG + chr4.1:14895424-14895443 MS.gene09233:CDS 35.0%
TGAGAGTAAACTTTATGCTG+CGG + chr4.1:14895592-14895611 MS.gene09233:CDS 35.0%
TTCTAAGCAACAGAAAGAGA+TGG + chr4.1:14895304-14895323 MS.gene09233:CDS 35.0%
TTTAGATGATTCACAACGAC+CGG - chr4.1:14895006-14895025 None:intergenic 35.0%
! AATTTTGTCTCTGCTGACAA+TGG + chr4.1:14895062-14895081 MS.gene09233:CDS 35.0%
! GAGAGAATTGAAAGTATGTC+GGG + chr4.1:14895641-14895660 MS.gene09233:CDS 35.0%
AACAATGCGAAGGTTGAAAG+CGG + chr4.1:14895341-14895360 MS.gene09233:CDS 40.0%
AACTTTATGCTGCGGAGAAA+AGG + chr4.1:14895600-14895619 MS.gene09233:CDS 40.0%
GGAGGCTATGATGTTATTCT+TGG + chr4.1:14895092-14895111 MS.gene09233:CDS 40.0%
GTGAGAAGGAGAAAATGCAA+AGG + chr4.1:14895423-14895442 MS.gene09233:CDS 40.0%
! CATTTTCTCCTTCTCACTCA+CGG - chr4.1:14895420-14895439 None:intergenic 40.0%
! GTTTAAGTTCCTTGTGAAGC+TGG - chr4.1:14895148-14895167 None:intergenic 40.0%
CTAAGCAATGATGCGAAGAG+TGG + chr4.1:14895509-14895528 MS.gene09233:CDS 45.0%
GATTCACAACGACCGGTAAT+CGG - chr4.1:14894999-14895018 None:intergenic 45.0%
GGGACAAGAACAACAGAAAG+AGG + chr4.1:14895475-14895494 MS.gene09233:CDS 45.0%
GTCCTAATTTCTATGTCCCG+AGG - chr4.1:14895548-14895567 None:intergenic 45.0%
GTCCTCGGGACATAGAAATT+AGG + chr4.1:14895543-14895562 MS.gene09233:CDS 45.0%
!! GGAGAAAATTTTGGCAGCTC+TGG - chr4.1:14895042-14895061 None:intergenic 45.0%
AATGCGAAGGTTGAAAGCGG+AGG + chr4.1:14895344-14895363 MS.gene09233:CDS 50.0%
AGCAATGATGCGAAGAGTGG+AGG + chr4.1:14895512-14895531 MS.gene09233:CDS 50.0%
ATGCGAAGGTTGAAAGCGGA+GGG + chr4.1:14895345-14895364 MS.gene09233:CDS 50.0%
CTTGCTCAACCAGCTTCACA+AGG + chr4.1:14895136-14895155 MS.gene09233:CDS 50.0%
GAATGAAGCCGTGAGTGAGA+AGG + chr4.1:14895409-14895428 MS.gene09233:CDS 50.0%
GCAATGATGCGAAGAGTGGA+GGG + chr4.1:14895513-14895532 MS.gene09233:CDS 50.0%
TCTGCTGACAATGGTGCTTC+AGG + chr4.1:14895071-14895090 MS.gene09233:CDS 50.0%
CTCCTCGGGATGTAGAAGTC+AGG + chr4.1:14895375-14895394 MS.gene09233:CDS 55.0%
GACCTGACTTCTACATCCCG+AGG - chr4.1:14895380-14895399 None:intergenic 55.0%
GCTGACAATGGTGCTTCAGG+AGG + chr4.1:14895074-14895093 MS.gene09233:CDS 55.0%
TCTATGTCCCGAGGACCCAT+CGG - chr4.1:14895539-14895558 None:intergenic 55.0%
!! CGGAGGGTTAGTTGCTCCTC+GGG + chr4.1:14895361-14895380 MS.gene09233:CDS 60.0%
!! GCGGAGGGTTAGTTGCTCCT+CGG + chr4.1:14895360-14895379 MS.gene09233:CDS 60.0%
GCGAAGAGTGGAGGGCCGAT+GGG + chr4.1:14895521-14895540 MS.gene09233:CDS 65.0%
TGCGAAGAGTGGAGGGCCGA+TGG + chr4.1:14895520-14895539 MS.gene09233:CDS 65.0%
GTGGAGGGCCGATGGGTCCT+CGG + chr4.1:14895528-14895547 MS.gene09233:CDS 70.0%
TGGAGGGCCGATGGGTCCTC+GGG + chr4.1:14895529-14895548 MS.gene09233:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 14894993 14895694 14894993 ID=MS.gene09233
chr4.1 mRNA 14894993 14895694 14894993 ID=MS.gene09233.t1;Parent=MS.gene09233
chr4.1 exon 14894993 14895694 14894993 ID=MS.gene09233.t1.exon1;Parent=MS.gene09233.t1
chr4.1 CDS 14894993 14895694 14894993 ID=cds.MS.gene09233.t1;Parent=MS.gene09233.t1
Gene Sequence

>MS.gene09233

ATGCCGATTACCGGTCGTTGTGAATCATCTAAAGAGATTTCGCTTTCCAGAGCTGCCAAAATTTTCTCCAATTTTGTCTCTGCTGACAATGGTGCTTCAGGAGGCTATGATGTTATTCTTGGAAAGACTTTCAAGGGTTTCAACTTGCTCAACCAGCTTCACAAGGAACTTAAACTATCAAAATCTCATAGGAAGAATCGTCAAAGCTACATAACAGAAGATAGTGTTGATGTGAAATCTGAAAAAGAGTTTCGCAATGTTGATGGGAAGTTGAATCAGAATGATGTTAAATTCAATCAAGAAGTTAATGGTTCTAAGCAACAGAAAGAGATGGAGAAAAAGATAAGTAACAATGCGAAGGTTGAAAGCGGAGGGTTAGTTGCTCCTCGGGATGTAGAAGTCAGGTCAAAAAAGAGGAATGAAGCCGTGAGTGAGAAGGAGAAAATGCAAAGGGACAAGAATTTAGAAGTTGAAAATGCGAAGGGACAAGAACAACAGAAAGAGGTAGAGAAAAAACTAAGCAATGATGCGAAGAGTGGAGGGCCGATGGGTCCTCGGGACATAGAAATTAGGACAAACAAGAAACTTGAAGTAGGGAGTGAGAGTAAACTTTATGCTGCGGAGAAAAGGAAGAATCAAGATGGTGAAGAGAGAATTGAAAGTATGTCGGGAGAACATTCTAAGAAGAAAATGAAACACTAG

Protein sequence

>MS.gene09233.t1

MPITGRCESSKEISLSRAAKIFSNFVSADNGASGGYDVILGKTFKGFNLLNQLHKELKLSKSHRKNRQSYITEDSVDVKSEKEFRNVDGKLNQNDVKFNQEVNGSKQQKEMEKKISNNAKVESGGLVAPRDVEVRSKKRNEAVSEKEKMQRDKNLEVENAKGQEQQKEVEKKLSNDAKSGGPMGPRDIEIRTNKKLEVGSESKLYAAEKRKNQDGEERIESMSGEHSKKKMKH