AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31433


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31433.t1 MTR_2g090660 87.879 264 11 3 1 264 1 243 6.68e-131 370
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31433.t1 AT1G03820 27.719 285 122 7 1 264 1 222 1.24e-12 65.9

Find 38 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAGTGACACAAGATTTC+TGG 0.224853 2.4:+9861921 MS.gene31433:CDS
GAGAAGTATAACAACGTTAA+TGG 0.257730 2.4:+9861982 MS.gene31433:CDS
TTTCCTTGGTTGGAGAATAT+TGG 0.318029 2.4:-9861645 None:intergenic
AACGTTAATGGATATGAATC+AGG 0.326674 2.4:+9861994 MS.gene31433:CDS
TCAAAATGAGTTTGAGACTA+TGG 0.340281 2.4:+9862062 MS.gene31433:CDS
ACCGGTGCTGGTGCCGGTGC+TGG 0.371432 2.4:-9861490 None:intergenic
GCAGCTGCAGGTTCTTGTGC+TGG 0.400119 2.4:-9861520 None:intergenic
GTCGAAACAGGAAACGGTTA+CGG 0.431424 2.4:+9861601 MS.gene31433:CDS
GGAAACGGTTACGGTCTCTA+TGG 0.434656 2.4:+9861610 MS.gene31433:CDS
ACAATCTCTGGTGCAGCTGC+AGG 0.454852 2.4:-9861532 None:intergenic
TCTTGTGCTGGTGCTGATAC+CGG 0.463050 2.4:-9861508 None:intergenic
TTGAGGGTCTTCAACATTGT+GGG 0.464139 2.4:-9861455 None:intergenic
GACACAAGATTTCTGGAGAA+TGG 0.469127 2.4:+9861928 MS.gene31433:CDS
GTATCAACTACTCTACCTTC+AGG 0.472495 2.4:+9861553 MS.gene31433:CDS
GCTGGTGCTGATACCGGTGC+TGG 0.477279 2.4:-9861502 None:intergenic
GCTGATACCGGTGCTGGTGC+CGG 0.484693 2.4:-9861496 None:intergenic
AGAGTAGTTGATACAATCTC+TGG 0.485770 2.4:-9861544 None:intergenic
AACAATTACAACAGCTACAA+TGG 0.499851 2.4:+9861847 MS.gene31433:CDS
CTGGAGATTGAGGTAATTGA+GGG 0.513332 2.4:-9861471 None:intergenic
TCCAGCACCGGCACCAGCAC+CGG 0.523732 2.4:+9861489 MS.gene31433:CDS
GCTGGAGATTGAGGTAATTG+AGG 0.526485 2.4:-9861472 None:intergenic
GGTTCTGGTGCTGGTCCTGA+AGG 0.535734 2.4:-9861568 None:intergenic
TTGTTGCGGAAGTTTGTTTG+AGG 0.543740 2.4:-9861781 None:intergenic
ATTGAGGGTCTTCAACATTG+TGG 0.549263 2.4:-9861456 None:intergenic
AGGGTCTTCAACATTGTGGG+TGG 0.554411 2.4:-9861452 None:intergenic
TAGCTTGAATTTGGGGTGAG+AGG 0.561749 2.4:-9861390 None:intergenic
TAGCCAATATTCTCCAACCA+AGG 0.580385 2.4:+9861642 MS.gene31433:CDS
AACAACAATTATGAGAGGAA+AGG 0.589017 2.4:+9861892 MS.gene31433:CDS
AATTATGAGAGGAAAGGAGA+AGG 0.592187 2.4:+9861898 MS.gene31433:CDS
AATGGATATGAATCAGGGAG+AGG 0.596788 2.4:+9862000 MS.gene31433:CDS
GGTGCCGGTGCTGGAGATTG+AGG 0.597375 2.4:-9861481 None:intergenic
ATTACCTCAATCTCCAGCAC+CGG 0.607846 2.4:+9861477 MS.gene31433:CDS
AGAGGAAGCACTGAAAATGA+AGG 0.608381 2.4:+9862018 MS.gene31433:CDS
TGTACACTTCATTGTTGTTG+CGG 0.612560 2.4:-9861795 None:intergenic
TTACAACAGCTACAATGGAA+AGG 0.631436 2.4:+9861852 MS.gene31433:CDS
ACGTTAATGGATATGAATCA+GGG 0.631473 2.4:+9861995 MS.gene31433:CDS
ACAATAACAACAATTATGAG+AGG 0.640620 2.4:+9861887 MS.gene31433:CDS
GAGAAGCAGCAAGAAGCACA+AGG 0.650203 2.4:+9862093 MS.gene31433:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATAAAAGCTACAAAAA+AGG + chr2.4:9861754-9861773 MS.gene31433:CDS 20.0%
!!! AAATCTGTTAATGTTGTTTT+TGG - chr2.4:9861673-9861692 None:intergenic 20.0%
! ACAATAACAACAATTATGAG+AGG + chr2.4:9861887-9861906 MS.gene31433:CDS 25.0%
AACAACAATTATGAGAGGAA+AGG + chr2.4:9861892-9861911 MS.gene31433:CDS 30.0%
AACAATTACAACAGCTACAA+TGG + chr2.4:9861847-9861866 MS.gene31433:CDS 30.0%
ACAAGAAAAAGAAAGAGACT+TGG - chr2.4:9861360-9861379 None:intergenic 30.0%
! AACGTTAATGGATATGAATC+AGG + chr2.4:9861994-9862013 MS.gene31433:CDS 30.0%
! ACGTTAATGGATATGAATCA+GGG + chr2.4:9861995-9862014 MS.gene31433:CDS 30.0%
! AGTCTCTTTCTTTTTCTTGT+TGG + chr2.4:9861360-9861379 MS.gene31433:CDS 30.0%
! GAGAAGTATAACAACGTTAA+TGG + chr2.4:9861982-9862001 MS.gene31433:CDS 30.0%
! TCAAAATGAGTTTGAGACTA+TGG + chr2.4:9862062-9862081 MS.gene31433:CDS 30.0%
!! GTTTTGTCTAGCTTGAATTT+GGG - chr2.4:9861401-9861420 None:intergenic 30.0%
!! TGTTTTGTCTAGCTTGAATT+TGG - chr2.4:9861402-9861421 None:intergenic 30.0%
!! TTTTGTCTAGCTTGAATTTG+GGG - chr2.4:9861400-9861419 None:intergenic 30.0%
!! TTTTTAGTCGAAACAGGAAA+CGG + chr2.4:9861595-9861614 MS.gene31433:CDS 30.0%
AATGAGTGACACAAGATTTC+TGG + chr2.4:9861921-9861940 MS.gene31433:CDS 35.0%
AATTATGAGAGGAAAGGAGA+AGG + chr2.4:9861898-9861917 MS.gene31433:CDS 35.0%
CCTGTTTCGACTAAAAATTG+AGG - chr2.4:9861592-9861611 None:intergenic 35.0%
TCGACTAAAAATTGAGGTTC+TGG - chr2.4:9861586-9861605 None:intergenic 35.0%
TTACAACAGCTACAATGGAA+AGG + chr2.4:9861852-9861871 MS.gene31433:CDS 35.0%
TTTCCTTGGTTGGAGAATAT+TGG - chr2.4:9861648-9861667 None:intergenic 35.0%
! AGAGTAGTTGATACAATCTC+TGG - chr2.4:9861547-9861566 None:intergenic 35.0%
! TGTACACTTCATTGTTGTTG+CGG - chr2.4:9861798-9861817 None:intergenic 35.0%
!!! CCTCAATTTTTAGTCGAAAC+AGG + chr2.4:9861589-9861608 MS.gene31433:CDS 35.0%
!!! TGTTGTTTTTGGAGTTTCCT+TGG - chr2.4:9861662-9861681 None:intergenic 35.0%
AATGGATATGAATCAGGGAG+AGG + chr2.4:9862000-9862019 MS.gene31433:CDS 40.0%
ATTGAGGGTCTTCAACATTG+TGG - chr2.4:9861459-9861478 None:intergenic 40.0%
CTGGAGATTGAGGTAATTGA+GGG - chr2.4:9861474-9861493 None:intergenic 40.0%
GTATCAACTACTCTACCTTC+AGG + chr2.4:9861553-9861572 MS.gene31433:CDS 40.0%
TAGCCAATATTCTCCAACCA+AGG + chr2.4:9861642-9861661 MS.gene31433:CDS 40.0%
TTGAGGGTCTTCAACATTGT+GGG - chr2.4:9861458-9861477 None:intergenic 40.0%
! AAAAATTGAGGTTCTGGTGC+TGG - chr2.4:9861580-9861599 None:intergenic 40.0%
! GACACAAGATTTCTGGAGAA+TGG + chr2.4:9861928-9861947 MS.gene31433:CDS 40.0%
! TTGTTGCGGAAGTTTGTTTG+AGG - chr2.4:9861784-9861803 None:intergenic 40.0%
!! AGAGGAAGCACTGAAAATGA+AGG + chr2.4:9862018-9862037 MS.gene31433:CDS 40.0%
!!! GTTTTTGGAGTTTCCTTGGT+TGG - chr2.4:9861658-9861677 None:intergenic 40.0%
ATTACCTCAATCTCCAGCAC+CGG + chr2.4:9861477-9861496 MS.gene31433:CDS 45.0%
GCTGGAGATTGAGGTAATTG+AGG - chr2.4:9861475-9861494 None:intergenic 45.0%
GTCGAAACAGGAAACGGTTA+CGG + chr2.4:9861601-9861620 MS.gene31433:CDS 45.0%
! TAGCTTGAATTTGGGGTGAG+AGG - chr2.4:9861393-9861412 None:intergenic 45.0%
AGGGTCTTCAACATTGTGGG+TGG - chr2.4:9861455-9861474 None:intergenic 50.0%
GGAAACGGTTACGGTCTCTA+TGG + chr2.4:9861610-9861629 MS.gene31433:CDS 50.0%
! GAGAAGCAGCAAGAAGCACA+AGG + chr2.4:9862093-9862112 MS.gene31433:CDS 50.0%
!! TCTTGTGCTGGTGCTGATAC+CGG - chr2.4:9861511-9861530 None:intergenic 50.0%
ACAATCTCTGGTGCAGCTGC+AGG - chr2.4:9861535-9861554 None:intergenic 55.0%
GCAGCTGCAGGTTCTTGTGC+TGG - chr2.4:9861523-9861542 None:intergenic 60.0%
!! GGTTCTGGTGCTGGTCCTGA+AGG - chr2.4:9861571-9861590 None:intergenic 60.0%
!! GCTGATACCGGTGCTGGTGC+CGG - chr2.4:9861499-9861518 None:intergenic 65.0%
!! GCTGGTGCTGATACCGGTGC+TGG - chr2.4:9861505-9861524 None:intergenic 65.0%
!! GGTGCCGGTGCTGGAGATTG+AGG - chr2.4:9861484-9861503 None:intergenic 65.0%
!! TCCAGCACCGGCACCAGCAC+CGG + chr2.4:9861489-9861508 MS.gene31433:CDS 70.0%
!! ACCGGTGCTGGTGCCGGTGC+TGG - chr2.4:9861493-9861512 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 9861340 9862134 9861340 ID=MS.gene31433
chr2.4 mRNA 9861340 9862134 9861340 ID=MS.gene31433.t1;Parent=MS.gene31433
chr2.4 exon 9861340 9862134 9861340 ID=MS.gene31433.t1.exon1;Parent=MS.gene31433.t1
chr2.4 CDS 9861340 9862134 9861340 ID=cds.MS.gene31433.t1;Parent=MS.gene31433.t1
Gene Sequence

>MS.gene31433

ATGGCTTCTCTTGCAAACCAAGTCTCTTTCTTTTTCTTGTTGGTACTTTTCCTCTCACCCCAAATTCAAGCTAGACAAAACAAGTTCTTTAGCTTCTTTACTCAATTCAAAACCACCCACAATGTTGAAGACCCTCAATTACCTCAATCTCCAGCACCGGCACCAGCACCGGTATCAGCACCAGCACAAGAACCTGCAGCTGCACCAGAGATTGTATCAACTACTCTACCTTCAGGACCAGCACCAGAACCTCAATTTTTAGTCGAAACAGGAAACGGTTACGGTCTCTATGGCATTGATTCTAGCCAATATTCTCCAACCAAGGAAACTCCAAAAACAACATTAACAGATTTTGAAGATGAGCTTCTCAATGAAGACTTCAATGATAATAAGCTTCTCAATGAAGATTTCAATGATAATAAAAGCTACAAAAAAGGATATCCTCAAACAAACTTCCGCAACAACAATGAAGTGTACACAAAAAACTACAACAATGAAGAGAACAAGAACAATTACAACAGCTACAATGGAAAGGAGTTTTACAACAACAATAACAACAATTATGAGAGGAAAGGAGAAGGAATGAGTGACACAAGATTTCTGGAGAATGGAAAGTACTATTACAATGTAAATAGTGAAAATGAGAAGTATAACAACGTTAATGGATATGAATCAGGGAGAGGAAGCACTGAAAATGAAGGTAACTATGAAAAAAATCAGTATCAAAATGAGTTTGAGACTATGGAAGAGTATGAGAAGCAGCAAGAAGCACAAGGTTACACATACACACCATGA

Protein sequence

>MS.gene31433.t1

MASLANQVSFFFLLVLFLSPQIQARQNKFFSFFTQFKTTHNVEDPQLPQSPAPAPAPVSAPAQEPAAAPEIVSTTLPSGPAPEPQFLVETGNGYGLYGIDSSQYSPTKETPKTTLTDFEDELLNEDFNDNKLLNEDFNDNKSYKKGYPQTNFRNNNEVYTKNYNNEENKNNYNSYNGKEFYNNNNNNYERKGEGMSDTRFLENGKYYYNVNSENEKYNNVNGYESGRGSTENEGNYEKNQYQNEFETMEEYEKQQEAQGYTYTP