AlfalfaGEDB | MS.gene059957

M. sativa cultivar XinJiangDaYe / MS.gene059957

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059957.t1	KEH31751.1	96.5	431	15	0	1	431	1	431	1.80E-244	854.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059957.t1	Q9STK8	52.9	431	192	5	1	428	65	487	2.1e-132	473.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059957.t1	A0A072UQW8	96.5	431	15	0	1	431	1	431	1.3e-244	854.7

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059957	MS.gene48303	0.988053	1.55E-172	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene00054	MS.gene059957	PPI
MS.gene00055	MS.gene059957	PPI
MS.gene00056	MS.gene059957	PPI
MS.gene003430	MS.gene059957	PPI
MS.gene008145	MS.gene059957	PPI
MS.gene008451	MS.gene059957	PPI
MS.gene008452	MS.gene059957	PPI
MS.gene008464	MS.gene059957	PPI
MS.gene008465	MS.gene059957	PPI
MS.gene008470	MS.gene059957	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059957.t1	MTR_4g104540	96.520	431	15	0	1	431	1	431	0.0	867
MS.gene059957.t1	MTR_4g104610	77.523	436	87	3	1	427	63	496	0.0	692
MS.gene059957.t1	MTR_4g104550	75.058	433	104	2	1	430	63	494	0.0	684
MS.gene059957.t1	MTR_4g089030	61.432	433	161	2	1	427	83	515	0.0	566
MS.gene059957.t1	MTR_4g089025	62.500	432	155	4	1	427	87	516	0.0	557
MS.gene059957.t1	MTR_0022s0290	58.824	442	169	5	1	429	76	517	0.0	538
MS.gene059957.t1	MTR_4g089020	59.028	432	167	4	1	427	65	491	0.0	533
MS.gene059957.t1	MTR_4g089055	57.639	432	173	4	1	427	87	513	1.04e-180	514
MS.gene059957.t1	MTR_4g104570	96.000	200	8	0	232	431	1	200	1.03e-142	405
MS.gene059957.t1	MTR_3g076530	46.636	431	220	5	3	428	77	502	4.03e-142	416

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059957.t1	AT3G48280	52.900	431	192	5	1	428	65	487	6.81e-167	478
MS.gene059957.t1	AT3G48270	51.860	430	195	5	1	427	66	486	1.14e-162	467
MS.gene059957.t1	AT3G48290	52.326	430	193	6	1	427	68	488	2.91e-157	453
MS.gene059957.t1	AT3G48310	51.860	430	195	6	1	427	67	487	3.66e-157	453
MS.gene059957.t1	AT3G48300	51.636	428	195	6	1	427	64	480	5.24e-155	447
MS.gene059957.t1	AT3G48300	51.636	428	195	6	1	427	66	482	5.60e-155	447
MS.gene059957.t1	AT3G48290	49.342	456	193	7	1	427	68	514	3.78e-151	439
MS.gene059957.t1	AT3G48320	51.740	431	194	7	1	427	67	487	7.90e-148	429
MS.gene059957.t1	AT4G13310	48.148	432	216	5	1	427	67	495	6.76e-143	417
MS.gene059957.t1	AT2G30770	47.917	432	215	6	1	427	68	494	1.04e-137	404

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Find 91 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTATGTCGTAATTTATTT+TGG	0.130051	8.1:-19973107	None:intergenic
AATGGTTACCACATCAAAGC+TGG	0.552341	8.1:+19974792	MS.gene059957:CDS
AATTGAGGGATGTAAACGTA+GGG	0.587311	8.1:-19974727	None:intergenic
ACCAGTTCTCGTGATCTCTT+CGG	0.481989	8.1:+19973027	MS.gene059957:CDS
ACCTAGTACATCAATTTAAC+TGG	0.251658	8.1:+19975018	MS.gene059957:CDS
ACCTGGTGGTGCTGAAGGAT+TGG	0.501420	8.1:+19975046	MS.gene059957:CDS
ACTATGTTCCTTGGCTTGAT+TGG	0.286633	8.1:+19973461	MS.gene059957:CDS
ACTGTGAGAGAGGAAGAAAC+GGG	0.614530	8.1:+19973241	MS.gene059957:CDS
AGAAAGTATAGTGGTGAAAA+TGG	0.251892	8.1:+19973376	MS.gene059957:CDS
AGACAAGATATCAAACTAAA+TGG	0.333728	8.1:+19974774	MS.gene059957:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGTCCATTAAGCTTCAA+AGG	-	chr8.1:19973725-19973744	None:intergenic	30.0%
AAAATCATACAAGTTCCTAG+AGG	-	chr8.1:19974407-19974426	None:intergenic	30.0%
AAATGTTGTATGTCATTAGG+CGG	+	chr8.1:19974437-19974456	MS.gene059957:intron	30.0%
ACCTAGTACATCAATTTAAC+TGG	+	chr8.1:19975018-19975037	MS.gene059957:CDS	30.0%
ATCTATCTTTGACGAAAGAA+AGG	+	chr8.1:19974179-19974198	MS.gene059957:intron	30.0%
ATCTTTCTGGATATTATGCA+AGG	+	chr8.1:19973491-19973510	MS.gene059957:CDS	30.0%
CATGAAATACTTAAATGCAG+TGG	+	chr8.1:19974695-19974714	MS.gene059957:CDS	30.0%
CCTAGTACATCAATTTAACT+GGG	+	chr8.1:19975019-19975038	MS.gene059957:CDS	30.0%
GAAAGAAGAAGTAAGTAAAG+TGG	+	chr8.1:19974632-19974651	MS.gene059957:CDS	30.0%
GATCATATCAATAATCCGAA+AGG	+	chr8.1:19973565-19973584	MS.gene059957:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	19973004	19975145	19973004	ID=MS.gene059957
chr8.1	mRNA	19973004	19975145	19973004	ID=MS.gene059957.t1;Parent=MS.gene059957
chr8.1	exon	19973004	19973693	19973004	ID=MS.gene059957.t1.exon1;Parent=MS.gene059957.t1
chr8.1	CDS	19973004	19973693	19973004	ID=cds.MS.gene059957.t1;Parent=MS.gene059957.t1
chr8.1	exon	19974540	19975145	19974540	ID=MS.gene059957.t1.exon2;Parent=MS.gene059957.t1
chr8.1	CDS	19974540	19975145	19974540	ID=cds.MS.gene059957.t1;Parent=MS.gene059957.t1

Gene Sequence

>MS.gene059957

ATGCGACTCAATTTTGGTCGTGTACCAGTTCTCGTGATCTCTTCGGCTGATGCAGCACATGAGATAATGAAAACACATGATCATGTCTTTGCAAACAGACCCCCCAAAATAAATTACGACATACTTTTATATAATTTCAGAGATGTTTCATCAGCTCCATATGGAGAGTATTGGAGGCAGTTAAGGAGCATATGTGTGTTACATCTTCTTAGTGCTAAAAGCGTTCGATCTTTTCGCACTGTGAGAGAGGAAGAAACGGGTTTAATGATGGAGAAAATAAAGCACTATTCTTCTTCCACTTCCTTACCAGTGAATTTAAGTGAATTAATGGCAACAACTACGAATGATATAGTTTGTAGGGTTGTTTTGGGAAGAAAGTATAGTGGTGAAAATGGGAAGGGATTTTCAAAGTTATTGATGGATTTTACTGAGTTACTTGGTGCTTTTGTTGTTGGGGACTATGTTCCTTGGCTTGATTGGTTTTCCCATCTTTCTGGATATTATGCAAGGGCAAAAAAAGTGGCTAAACAATTTGATGATCTTTTGGAAGGTGTTGTTGAAGATCATATCAATAATCCGAAAGGAGATAGTAACGAGCATGAGGACTTTGTTGAAGTTTTGCTTTGGATCCAAAGAACAGAATCACTTGGCTTTCCTATTGACAAAACGGTCATAAAGGCTCTTTTATTGGTAAGTGTAAAACTAAAATCTCTATCATCCTTTGAAGCTTAATGGACTTTTTTTCGAGAAGTAAAAAATTTATTAAAGGGTCTTTTTGTACATTTATCTAATCATGTTTAATTATAGTATAAGTTTGTTGATATATTATATTTTTTAAAAAATATTATACCAAATTAATACACTTATACATAAGTGTTTTGGTGTAGTACTTTTCTTCCAACTATAACATTCGTTAAAATTATTGTTTTTATTTACAATCTTATCCTTCTTATAATCTTATTTAAATCTTTAATTAAAAAAAATCACTTAAATATTATTATAATATGAGCAATCCCATCACAATAGGAATAATATAAATTAAATCTTTATTTGATCATGGTATATTCCAATAAAAAAATTATAACCAAGTACAATAAAATAAATATTCATTCAAGTATGATAGAAGAAGATGGACATATTAGTATCGTCTTGACGTACACTAATTAGCCGGTTATATCTATCTTTGACGAAAGAAAGGAATCTTTGACCTAAGGGTTAAGCTACTAAAGTCGTCCTTTTAGGTCTACTAACAAAATATTATTTTTTATTAGATATTATAGACAAAAAGCCGCTTATGCAAACGAAAAAAGCTCATATATTAAAACTTGTTTTAAGCCTCACTCACAATTAAAAATTATTGGACTGGCCCTGCGCATCATATTAATATAATGTTTGAGACACCTCTAGGAACTTGTATGATTTTCCATGCTTGCAAATGTTGTATGTCATTAGGCGGTGTTATGTTTTAATTGTAATGATGATAAATTATAATTTGATCATGCTAACTTTATTGATCTTGTATTTGTTTCTATTCAGGACATGTTTATTGCAGGTACAGACACCATATCCACTTTGCTAGAGTGGTCAATGACAGAACTCTTAAGGCATCCAAATACCATGAAGAAATTGAAAGAAGAAGTAAGTAAAGTGGCTAATGGTAGAACACACATAACTGAAGAAGATTTGAGTCACATGAAATACTTAAATGCAGTGGTTAAAGAAACCCTACGTTTACATCCCTCAATTCCATTACTAGTACCTCGAGAAAGCAGACAAGATATCAAACTAAATGGTTACCACATCAAAGCTGGCACACGAGTTTTCATCAATGCTTGGGCCATTGCAAGAGATCCTACACATTGGGATCAACCTGAAGAGTTCAAGCCTGAGAGATTCTTGAGTAGTTCAATAGATGTTAAAGGAAAAGATTTCCAAGTGATTCCATTTGGAGCTGGTAGAAGGGGTTGTCCTGGGGCAGTGTATGCTACGGCTGTCAATGATCTTGTGTTAGCCAACCTAGTACATCAATTTAACTGGGAATTACCTGGTGGTGCTGAAGGATTGGACATGTCTGAATCTTTTGGATTCACAGTTCATAGAAAGATTCCTCTTATGGCAATTGCAACCCCTAACAAGAACTAA

Protein sequence

>MS.gene059957.t1

MRLNFGRVPVLVISSADAAHEIMKTHDHVFANRPPKINYDILLYNFRDVSSAPYGEYWRQLRSICVLHLLSAKSVRSFRTVREEETGLMMEKIKHYSSSTSLPVNLSELMATTTNDIVCRVVLGRKYSGENGKGFSKLLMDFTELLGAFVVGDYVPWLDWFSHLSGYYARAKKVAKQFDDLLEGVVEDHINNPKGDSNEHEDFVEVLLWIQRTESLGFPIDKTVIKALLLDMFIAGTDTISTLLEWSMTELLRHPNTMKKLKEEVSKVANGRTHITEEDLSHMKYLNAVVKETLRLHPSIPLLVPRESRQDIKLNGYHIKAGTRVFINAWAIARDPTHWDQPEEFKPERFLSSSIDVKGKDFQVIPFGAGRRGCPGAVYATAVNDLVLANLVHQFNWELPGGAEGLDMSESFGFTVHRKIPLMAIATPNKN