AlfalfaGEDB | MS.gene63500

M. sativa cultivar XinJiangDaYe / MS.gene63500

KEGG: -
GO: -
PFAM: PF00415.21 PF13540.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63500.t1	XP_003600756.1	97.6	464	11	0	1	464	1	464	2.30E-237	831.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63500.t1	Q9FN03	35.3	249	138	4	214	461	119	345	7.9e-37	156.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63500.t1	G7J9Y3	97.6	464	11	0	1	464	1	464	1.6e-237	831.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene016104	MS.gene63500	PPI
MS.gene052501	MS.gene63500	PPI
MS.gene052820	MS.gene63500	PPI
MS.gene052823	MS.gene63500	PPI
MS.gene052825	MS.gene63500	PPI
MS.gene27308	MS.gene63500	PPI
MS.gene29986	MS.gene63500	PPI
MS.gene33766	MS.gene63500	PPI
MS.gene37842	MS.gene63500	PPI
MS.gene39429	MS.gene63500	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63500.t1	MTR_3g069030	97.845	464	10	0	1	464	1	464	0.0	920
MS.gene63500.t1	MTR_7g063440	61.255	462	164	5	13	463	24	481	0.0	569
MS.gene63500.t1	MTR_7g063440	59.804	408	149	5	13	409	24	427	1.97e-167	478
MS.gene63500.t1	MTR_7g063440	29.680	219	122	5	266	459	215	426	9.73e-17	82.4
MS.gene63500.t1	MTR_7g063440	60.207	387	140	4	87	463	7	389	4.81e-164	468
MS.gene63500.t1	MTR_7g063440	27.526	287	168	7	201	457	4	280	6.00e-14	73.6
MS.gene63500.t1	MTR_8g094410	32.400	250	143	4	203	449	24	250	4.41e-38	141
MS.gene63500.t1	MTR_8g094410	34.615	156	98	2	308	462	5	157	8.02e-23	99.0
MS.gene63500.t1	MTR_8g094410	37.736	106	64	1	357	462	2	105	2.52e-19	89.0
MS.gene63500.t1	MTR_8g094410	27.586	377	222	10	85	449	47	384	2.24e-37	141

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63500.t1	AT3G55580	70.021	467	118	6	8	461	28	485	0.0	643
MS.gene63500.t1	AT3G53830	59.916	474	160	5	7	464	13	472	0.0	577
MS.gene63500.t1	AT3G53830	57.582	488	177	5	7	464	13	500	0.0	565
MS.gene63500.t1	AT3G53830	59.330	418	140	5	7	408	13	416	1.50e-173	494
MS.gene63500.t1	AT3G53830	32.099	162	78	4	329	460	204	363	1.02e-14	76.3
MS.gene63500.t1	AT3G53830	56.713	432	157	5	7	408	13	444	6.98e-168	480
MS.gene63500.t1	AT3G53830	32.099	162	78	4	329	460	232	391	1.71e-14	75.9
MS.gene63500.t1	AT5G63860	29.067	375	220	9	85	449	49	387	4.35e-39	147
MS.gene63500.t1	AT5G63860	34.601	263	147	5	200	461	107	345	7.72e-39	146
MS.gene63500.t1	AT5G63860	33.730	252	143	5	213	462	13	242	2.29e-37	142

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Find 165 sgRNAs with CRISPR-Local

Find 402 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTTGCATGTGGTTGG+TGG	0.620749	3.1:+56552373	MS.gene63500:CDS
AACAAGGAAAGCTTATAACA+TGG	0.617045	3.1:+56547923	MS.gene63500:CDS
AACTGGTTCTGATCAAGCTG+AGG	0.643001	3.1:+56550761	MS.gene63500:CDS
AAGATAACAAGTGTTGCTGC+CGG	0.614403	3.1:+56549068	MS.gene63500:CDS
AAGGACGCCGTTATTGACGC+CGG	0.695358	3.1:+56547087	MS.gene63500:CDS
AATAAGGAAGATACGCAGGT+CGG	0.704228	3.1:-56550633	None:intergenic
AATATCATCACCAGATGATG+AGG	0.594292	3.1:-56549003	None:intergenic
AATGGAAAAGTCTTCAGCTG+GGG	0.654614	3.1:+56552078	MS.gene63500:CDS
AATGTAAAGACCATATCTTG+TGG	0.721619	3.1:+56551199	MS.gene63500:CDS
AATTGGAAGGATGTTTCTGG+AGG	0.614754	3.1:+56547145	MS.gene63500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAGGAAAGCTTATAACA+TGG	+	chr3.1:56547923-56547942	MS.gene63500:CDS	30.0%
AACAATCTTTGACCAAACAT+CGG	+	chr3.1:56547743-56547762	MS.gene63500:intron	30.0%
AACATATATAGGAAGTAAGG+GGG	+	chr3.1:56551744-56551763	MS.gene63500:intron	30.0%
AATAATTTACACTGCAAGCT+AGG	-	chr3.1:56549968-56549987	None:intergenic	30.0%
AATGTAAAGACCATATCTTG+TGG	+	chr3.1:56551199-56551218	MS.gene63500:CDS	30.0%
AATTCAACTGTCATTAAGAG+GGG	-	chr3.1:56551948-56551967	None:intergenic	30.0%
AATTGTCAACTTGAATTACG+AGG	+	chr3.1:56551131-56551150	MS.gene63500:intron	30.0%
ACAAGGAAAGCTTATAACAT+GGG	+	chr3.1:56547924-56547943	MS.gene63500:CDS	30.0%
ATAAAAAAACTCATGAGGGT+AGG	-	chr3.1:56550361-56550380	None:intergenic	30.0%
ATAAGACTTCACAATTCTCT+TGG	-	chr3.1:56551859-56551878	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	56547013	56552428	56547013	ID=MS.gene63500
chr3.1	mRNA	56547013	56552428	56547013	ID=MS.gene63500.t1;Parent=MS.gene63500
chr3.1	exon	56547013	56547196	56547013	ID=MS.gene63500.t1.exon1;Parent=MS.gene63500.t1
chr3.1	CDS	56547013	56547196	56547013	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56547923	56547996	56547923	ID=MS.gene63500.t1.exon2;Parent=MS.gene63500.t1
chr3.1	CDS	56547923	56547996	56547923	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56548079	56548163	56548079	ID=MS.gene63500.t1.exon3;Parent=MS.gene63500.t1
chr3.1	CDS	56548079	56548163	56548079	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56548539	56548670	56548539	ID=MS.gene63500.t1.exon4;Parent=MS.gene63500.t1
chr3.1	CDS	56548539	56548670	56548539	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56548898	56549116	56548898	ID=MS.gene63500.t1.exon5;Parent=MS.gene63500.t1
chr3.1	CDS	56548898	56549116	56548898	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56549267	56549482	56549267	ID=MS.gene63500.t1.exon6;Parent=MS.gene63500.t1
chr3.1	CDS	56549267	56549482	56549267	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56549869	56549912	56549869	ID=MS.gene63500.t1.exon7;Parent=MS.gene63500.t1
chr3.1	CDS	56549869	56549912	56549869	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56550600	56550782	56550600	ID=MS.gene63500.t1.exon8;Parent=MS.gene63500.t1
chr3.1	CDS	56550600	56550782	56550600	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56551157	56551247	56551157	ID=MS.gene63500.t1.exon9;Parent=MS.gene63500.t1
chr3.1	CDS	56551157	56551247	56551157	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56552076	56552119	56552076	ID=MS.gene63500.t1.exon10;Parent=MS.gene63500.t1
chr3.1	CDS	56552076	56552119	56552076	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1
chr3.1	exon	56552306	56552428	56552306	ID=MS.gene63500.t1.exon11;Parent=MS.gene63500.t1
chr3.1	CDS	56552306	56552428	56552306	ID=cds.MS.gene63500.t1;Parent=MS.gene63500.t1

Gene Sequence

>MS.gene63500

ATGAATGGTGAAGGGTGTGAAATGAAGGAGAAAATGGTATACATGTGGGGTTATTTACCGGGAGCTTTACCGCAAAGGACGCCGTTATTGACGCCGGTACTTGTTAGGGTTCCAGCGTCGTCTGGTGGTTATAATTGGAAGGATGTTTCTGGAGGTGGTTGTGGATTTGCCATGGCTATATCTGGTATTTTACCATTTTCAATTGAGTTTGAATTTTTTGTTGTTATGTTCATGTATAGTGTTGATATGAAGCTTGAAATGATGAAATTTGCATTGAGTGTTTCATTTCCATTTTAATTAAGTGAGAAATTCATTGAGATATTTTCAGTTATTCTTAGTAATAGATGTGTTTGTTTGATTGTGTGGTTTTGTTGGCATCAAAGTTTCTTAGCCTAATTTTTAAATTTTATTGTTATTATTTTTTAATTTAGTTAATTAAGTAAATGGTCTCTGTCAATATTTTTTAATTCTTACAACAAAAAATTTACACTTTTTAGTCCTTAACGTCATCATTTTTAGTTCTTGTAAAATTTTCCATCGGCAGTTCTTGTCCCGACTAAATTGTCCATTAAAAATGCTACGGAACTAAAAAGTGCGGTTTTTTTGTAAGAACTAAAAGTGAAATTCTGGTTATGAATATCTACGGAGACCATTTACATAATTAATCTTTTTTAATTTAAATGATTTCAATCAAGGTCGAATTGATTTGGAGCATGAAATTCAATTCTGCAACAATCTTTGACCAAACATCGGGCTGAATTCCGTATTACCAGCCATGAACGTTTTTTTGAGGAAGTTATGGCAATGCATATCTTTCCGTCACTCTATCTCTTTAAGCATTTTCTTTTTACATTTTATTATTGAAAACTGTAAATGATTCATCATTGCTCATATTTTCTTCCTTAATCAGAACAAGGAAAGCTTATAACATGGGGTTCGACAGATGATCTAGGCCAAAGTTATGTGACTTCGGGCAAACACGGGGTAATGTTTAAGCATATTGAATTCTACTGTCTCTCTTGGCTTTTTGTCGGTTAACCAACATTTTATAATTTTGTTTGTAAAGGAAACTCCAGAGCCTTTCCCTCTTCCAAACGAAGTGTCTATTGTAAAAGCTGCTTCTGCGTGGGCACATTGTGTTGCTGCAACGGGTAAGCTTTGTTTCTTTACATTGCTTGCCTGAGAAAAAAATCAATGTGATTGAAAGGACAAGGGATAAATTAAAATTGAATCAGTGGTAATGTCTTGCTGGAGAAATCATCCAGTGCTTACTGACTGTTAATTTCATGAATATTGTAGCTGATGATGAAAGTATTATTGGCATGAGCTGTAACAACATTATGCGCACGAGTTGTTACGGCGTTGAAGAATGTGCCTAAATGCTAATCTGAAGCCTCTCTTCATTTTGATTCAAAACAAAAAATCATGAATATCAAATAGGATTCAAGAAATTATATTTATCTGGGTAAGAGATTGTATGTCTTACTGGCATCATCTTACAAGTCTATTCTGCTGTCCTGTTGTAGATTATGGAGAAGTTTACACATGGGGATGGAAGGAATGTATTCCTTCTGGAAAGGTATTCGGTGAAACTTCGCAAGGGGTAAGTCCTGAAAAAGATGCACAAGGAAAGCAGAGTTCATTCTTGACAGAACAAGGTATGTCTAACCTCTTTACAATGGACATTTGTCTACAGATACCTGCCCCTCCCAGGAGGTGGTCTAAGCCAAAGGTCTACAAAAAGCATTCTGGAGCAGGGAACTGATTTAAGATACAAAGTTCGAAACCCCAAAAAGAAAGAAGAAAAGAGTTTTGCACTTTATGATGAGCAAAGAAGGATACTTATGCTTTATCTAACAAACTGATTAGAGTGATTTTGCAACAGTGAGCTCTCGTTCTCAAGTCTCAAAATCCACAGGAGGAACTGTTTCAAGTGGAGAAGAAAGTACTAAGAGAAGGAAAGTATCTTCAGCAAAGCAAGCGGCTCAAACCTCATCATCTGGTGATGATATTCTAACAGCAATGCCATGTCTTGTCACACTAAATCCAGGGGTAAAGATAACAAGTGTTGCTGCCGGTGGGCGTCATACCCTAGTATTGTCAGGTATTGACTTTATATGGACTATTTATTCTCCCGAGCTTAAAATTTCATGTAATGTAACCATCTTTTGGTTGAAGCATCACTTTTATGCTATGGAAGATCAGACATGCAGTCTTAGACATGTCATTAATTTTAACTTTCTGAATTTTTCAGATATAGGACAGGTGTGGGGTTGGGGCTATGGAGGTGAAGGGCAGCTTGGTTTGGGCTCCAGAGTACGTATGGTGTCCTCTCCTCATATTATTCCTTGCATTGATTCCTCTTCACTGGTTCAGGGAAGCATGAGTTCAGAAGGACACAATTTTAGGATACCTGGAAGTTGTATAAAGGCTATTGCCTGTGGTGGTCGACATAGTGCAGTAATCACAGGTGCGTGTGTGTGTCAGTCTTTTTGTGTCACAAATAAAAATCTTTCAGGAAGCCTGCATTAGTTTCTTTGGGGAACCAACAATGAGAATATCCATATGCTTGTCAGGATTGGTGGTTAAAAGTGTTTAGAAAGAGAAAATGTGTCTATGTGTACAATCAGATATTGAATTGTATGATTTAATGAGAGGGAGAAACAACAACAGGTCCTTCAATCAGTGAAAATATCAATAAATCTTGTCTTTCTTAATGCTAGATTAATAACTGAATAATTAATCTATTGAAAATTAAACAAATTCCATAGTTTCTTATCGTGTCCTAGAATTGTTCTCAACTATTTTTGGTCATTTACATACTGATTTTCAAATATATGCCTGCTGAAATGACAGATGCTGGAGCTGTGCTAGCGTTTGGTTGGGGACTTTATGGACAGGTGATTTAAAGGGACTAGCGATACTTATGGAGCGTTTTCTCCACTTACATATCCTAGCTTGCAGTGTAAATTATTCAGTTATCTGTATATTCCCTATCTTATGTGGTTACGTAACTAAGGATATTATTACCCAGCATTACACATGCAGAGTTTACCTTTTTAACAAGTTTCGACTGTATTTATTGTAAATAAAATCCCCTGGTGTGTTCTTACCTGTGGTAGTTTGTTTGGATCTTAATTTTAGAAAGCATATTGCAGTGCTGAATTCCTCCCAGGGAAGACCATGCAGTTCACCTTACTGGTCTTCATGTGTTTTCTTGATACGAGATGTATCAGCCGTTTGCTCTGTATTGGTGTTAACTTTATTGCTTAATTTGCTTGTCACCAACATCTTTTTAATTTTGCTGTCTAATACTTCCTTGGCACTGTGTATTTACAAAAGGCACCTACCCTCATGAGTTTTTTTATTTGCTTACAACAAGATTCTTGTTTTCAACTTTTAACCATTCTTTAGGATCAACATGTAGGTAAGTAGAATATGAGAGACCAAAAGCAATGTAGATGTTGAAGATGAGGTTCACTCTAACAAGGGATAATCTGTTCCAATATTTATATTCTTTATCGCTTTCTTTGTTGATGCATGCTTTTGCTTGTTATACTAAATTTTGTTATCTTGTTTTTGATAAGTGCGGGCAAGGAAGCACCGACGATGAACTGAGTCCGACCTGCGTATCTTCCTTATTGGGCATTCAAATAGAGGGAGTTGCTGCAGGTTTGTGGCATACTGTCTGTACATCTGCTGATGGTGATGTATATGCATTTGGTGGGAACCAGTTTGGGCAGCTGGGAACTGGTTCTGATCAAGCTGAGGTACGCCCTAGCCATCCCACAGATGACCCACACCCCCACTCTTATTGTCACTTAAATTTGAAACAAAACACAATGGTATGATTATGATTTATGAGCCCATTTGTTTGAATTTTACTTAAAAAATCTTGTATAAAAACTCAGTTTGTAAGAATTCGTAGGAGGTTTGTCTTGAGCGTTTCTGAAGTCAGAATCTCTAAAAAAGAACTTTTACACAATCTAAAAACCACATTTTTTAAGCTTAACAAATGGATCTTATATTTTTGTCCAGTGTACCATGACTATATCTTTATTGATGTCACCTTAGGCTACATACTCAGTTTTAATGAGGTATTATGTGTATTAGTTTTTAATTGTCAACTTGAATTACGAGGCAGACTCTACCAAGACTACTGGATTGTCCGAGCCTGGAAAATGTGAATGTAAAGACCATATCTTGTGGGGCCCGTCACACTGCTCTAGTGACAGGTATGCAACCTGTGTATGTCATTTCGGTATCTTCATTGGCAAAGCAATCGTTCCACTGTTGAATTTACTTTAGTGCATCTCTAAAGGCTGCTAGACTTGAATTCATGTATAATTTCTCAATAAAATTCCTTAAAATGAATTCTACAAGGTACTTCGATAAAGTACTGAAATCTTAATACTTGCATCTTCCTTTTGTTTGTTTGTTCCGAATTGGATTCCATCAAAAGAGACTGGGTTTTTCCCTCAAGTTTAGTTACATTATCTTTTGCCAATACAACCAACTAGAGCGACGGAAAATAATGGGACTGTTTTTTATTTCCTTTGTAAAAGATGAAGGATGCTGGTGCTATTGTGAAATGGTGGAGGTATATTCTTTAATCTTGTGATGTTGATATCAGACCTCACAGTAAGAGGGTCTGTCTGAAAAGGGATAAAGAAGAGTAGTTAATGATGATGTAATACTAACTATTAAAATTAACAAAATGTAACATCACAGAACATATATAGGAAGTAAGGGGGCAAGCAATTATAAGTAGAAGGGAAAAGAGAAGATAGAGGGTGGAGAAGTAACAACCGAGTGAAGAGAGTTTTAGGTGCTCTCTAATTAACCAAGAGAATTGTGAAGTCTTATCCTAGTTACATTGGCAACCAATGTTCATACAATTACTCTAGCTGTGTTTCCTTTACTCTTTTCTTCCCCCTCTTAATGACAGTTGAATTTATCAAAAGTCTACAATGACATTCATGCTAGTTTAGTTATTGTAAAATGTAGTAATCCCTATCATTAATAGATTATCACCAGTTTTCCTTTTGTTTTTGTTCTTCAAGATAATGGAAAAGTCTTCAGCTGGGGATGGAACAAATATGGACAGGTGAGTATATTGCGCTGCCATTTTTATTTATCTCCTTTTTATTTCATATTTTAAAAATGAACATTATTGAAAGTTCTACCTGACATATGTATTTAAGTGATAATGTTCTGGTTTGGTGCAATAATGGAATATATAATTGGTATGGTATTATAGTGTTGTCTAAGTCACAATTTGTGGTTGTGACAGCTCGGCCTTGGGGACGTGACTGACCGAAACATTCCATCTGAAGTCGCAATAGAAGGTTGTGTAGCTAAAAACGTTGCATGTGGTTGGTGGCACACTCTTCTTTTGGCCGAGTCACCGACTTGA

Protein sequence

>MS.gene63500.t1

MNGEGCEMKEKMVYMWGYLPGALPQRTPLLTPVLVRVPASSGGYNWKDVSGGGCGFAMAISEQGKLITWGSTDDLGQSYVTSGKHGETPEPFPLPNEVSIVKAASAWAHCVAATDYGEVYTWGWKECIPSGKVFGETSQGVSPEKDAQGKQSSFLTEQVSSRSQVSKSTGGTVSSGEESTKRRKVSSAKQAAQTSSSGDDILTAMPCLVTLNPGVKITSVAAGGRHTLVLSDIGQVWGWGYGGEGQLGLGSRVRMVSSPHIIPCIDSSSLVQGSMSSEGHNFRIPGSCIKAIACGGRHSAVITDAGAVLAFGWGLYGQCGQGSTDDELSPTCVSSLLGIQIEGVAAGLWHTVCTSADGDVYAFGGNQFGQLGTGSDQAETLPRLLDCPSLENVNVKTISCGARHTALVTDNGKVFSWGWNKYGQLGLGDVTDRNIPSEVAIEGCVAKNVACGWWHTLLLAESPT