Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062430.t1 | XP_003621899.2 | 96.6 | 176 | 6 | 0 | 1 | 176 | 1 | 176 | 1.40E-86 | 328.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062430.t1 | P05332 | 28.4 | 169 | 113 | 3 | 8 | 169 | 8 | 175 | 2.4e-10 | 67.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062430.t1 | G7KXV2 | 96.6 | 176 | 6 | 0 | 1 | 176 | 1 | 176 | 1.0e-86 | 328.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene062430.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene062430 | 0.8183 | 2.11E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene062430 | 0.865418 | 5.99E-65 | -1.69E-46 |
| MS.gene049669 | MS.gene062430 | 0.832039 | 1.19E-55 | -1.69E-46 |
| MS.gene049993 | MS.gene062430 | 0.804434 | 2.16E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene062430 | 0.801944 | 7.09E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene062430 | 0.817052 | 4.03E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene062430 | 0.819711 | 1.01E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene062430 | 0.80927 | 2.06E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene062430 | -0.811463 | 6.91E-51 | -1.69E-46 |
| MS.gene050642 | MS.gene062430 | 0.834697 | 2.58E-56 | -1.69E-46 |
| MS.gene050750 | MS.gene062430 | 0.819401 | 1.18E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene062430 | 0.834152 | 3.54E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene062430 | 0.85134 | 9.46E-61 | -1.69E-46 |
| MS.gene052077 | MS.gene062430 | 0.803872 | 2.83E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene062430 | 0.849294 | 3.55E-60 | -1.69E-46 |
| MS.gene052333 | MS.gene062430 | 0.810198 | 1.30E-50 | -1.69E-46 |
| MS.gene052389 | MS.gene062430 | 0.835149 | 1.99E-56 | -1.69E-46 |
| MS.gene052586 | MS.gene062430 | 0.823912 | 1.07E-53 | -1.69E-46 |
| MS.gene053166 | MS.gene062430 | 0.810991 | 8.75E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene062430 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene054083 | MS.gene062430 | 0.807818 | 4.20E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene062430 | 0.811574 | 6.54E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene062430 | 0.826237 | 3.03E-54 | -1.69E-46 |
| MS.gene054559 | MS.gene062430 | 0.808596 | 2.87E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene062430 | 0.818551 | 1.85E-52 | -1.69E-46 |
| MS.gene054942 | MS.gene062430 | 0.803108 | 4.08E-49 | -1.69E-46 |
| MS.gene055225 | MS.gene062430 | 0.80542 | 1.35E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene062430 | 0.811095 | 8.31E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene062430 | 0.81477 | 1.30E-51 | -1.69E-46 |
| MS.gene055367 | MS.gene062430 | 0.832711 | 8.09E-56 | -1.69E-46 |
| MS.gene055701 | MS.gene062430 | 0.854544 | 1.15E-61 | -1.69E-46 |
| MS.gene056506 | MS.gene062430 | 0.808328 | 3.27E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene062430 | 0.843265 | 1.56E-58 | -1.69E-46 |
| MS.gene057026 | MS.gene062430 | 0.808987 | 2.36E-50 | -1.69E-46 |
| MS.gene057156 | MS.gene062430 | -0.82423 | 9.03E-54 | -1.69E-46 |
| MS.gene057639 | MS.gene062430 | 0.807008 | 6.23E-50 | -1.69E-46 |
| MS.gene058142 | MS.gene062430 | 0.800922 | 1.15E-48 | -1.69E-46 |
| MS.gene058146 | MS.gene062430 | 0.807413 | 5.12E-50 | -1.69E-46 |
| MS.gene058435 | MS.gene062430 | 0.846385 | 2.25E-59 | -1.69E-46 |
| MS.gene059247 | MS.gene062430 | 0.809184 | 2.14E-50 | -1.69E-46 |
| MS.gene059710 | MS.gene062430 | 0.813549 | 2.42E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene062430 | 0.812527 | 4.05E-51 | -1.69E-46 |
| MS.gene060879 | MS.gene062430 | 0.834196 | 3.45E-56 | -1.69E-46 |
| MS.gene060998 | MS.gene062430 | 0.814211 | 1.73E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene062430 | -0.824332 | 8.55E-54 | -1.69E-46 |
| MS.gene061114 | MS.gene062430 | -0.83694 | 6.97E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062430.t1 | MTR_7g024760 | 97.159 | 176 | 5 | 0 | 1 | 176 | 1 | 176 | 6.90e-129 | 358 |
| MS.gene062430.t1 | MTR_7g024765 | 83.237 | 173 | 29 | 0 | 1 | 173 | 1 | 173 | 3.78e-105 | 299 |
| MS.gene062430.t1 | MTR_7g024780 | 69.364 | 173 | 52 | 1 | 1 | 172 | 1 | 173 | 6.50e-85 | 248 |
| MS.gene062430.t1 | MTR_7g114410 | 59.195 | 174 | 69 | 2 | 1 | 172 | 1 | 174 | 7.30e-69 | 207 |
| MS.gene062430.t1 | MTR_7g114410 | 58.228 | 158 | 64 | 2 | 1 | 156 | 1 | 158 | 2.45e-59 | 182 |
| MS.gene062430.t1 | MTR_3g463610 | 51.786 | 168 | 76 | 2 | 7 | 169 | 6 | 173 | 6.79e-54 | 171 |
| MS.gene062430.t1 | MTR_1g103110 | 45.732 | 164 | 86 | 3 | 7 | 167 | 22 | 185 | 1.49e-42 | 140 |
| MS.gene062430.t1 | MTR_5g006470 | 43.373 | 166 | 91 | 2 | 7 | 169 | 20 | 185 | 7.61e-40 | 134 |
| MS.gene062430.t1 | MTR_5g006460 | 43.275 | 171 | 88 | 6 | 7 | 169 | 21 | 190 | 8.58e-40 | 134 |
| MS.gene062430.t1 | MTR_5g006510 | 42.857 | 168 | 90 | 4 | 7 | 169 | 22 | 188 | 2.67e-39 | 132 |
| MS.gene062430.t1 | MTR_1g103150 | 42.771 | 166 | 92 | 3 | 7 | 169 | 22 | 187 | 8.84e-39 | 131 |
| MS.gene062430.t1 | MTR_5g006450 | 42.262 | 168 | 92 | 3 | 7 | 169 | 20 | 187 | 2.50e-38 | 130 |
| MS.gene062430.t1 | MTR_5g006520 | 41.071 | 168 | 94 | 3 | 7 | 169 | 1738 | 1905 | 2.97e-33 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062430.t1 | AT2G32020 | 41.040 | 173 | 98 | 3 | 1 | 171 | 12 | 182 | 3.28e-45 | 147 |
| MS.gene062430.t1 | AT3G22560 | 44.444 | 171 | 91 | 3 | 3 | 169 | 2 | 172 | 4.28e-44 | 144 |
| MS.gene062430.t1 | AT2G32030 | 40.361 | 166 | 95 | 3 | 8 | 171 | 24 | 187 | 2.39e-42 | 140 |
Find 49 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTATTGGGTTCAACTATT+GGG | 0.213071 | 7.3:+75312444 | MS.gene062430:CDS |
| GCAAAGTTTAAAGCAGATTT+AGG | 0.222691 | 7.3:+75312418 | MS.gene062430:CDS |
| ATGCTATTGGGTTCAACTAT+TGG | 0.239859 | 7.3:+75312443 | MS.gene062430:CDS |
| TCCTGATTTGGTAAGGTTGC+AGG | 0.261225 | 7.3:+75312525 | MS.gene062430:CDS |
| GCAGATTTAGGCTATGCTAT+TGG | 0.284212 | 7.3:+75312430 | MS.gene062430:CDS |
| ATTCCAAGACTTTCCTGATT+TGG | 0.305046 | 7.3:+75312513 | MS.gene062430:CDS |
| TGTCTTAATGATCACTCTAT+TGG | 0.321892 | 7.3:+75312367 | MS.gene062430:CDS |
| CAGATTTAGGCTATGCTATT+GGG | 0.336174 | 7.3:+75312431 | MS.gene062430:CDS |
| CTTTGAGAAGCCATGTTCTC+TGG | 0.364308 | 7.3:-75312559 | None:intergenic |
| TTCTGAACAATCCCTCCCTT+TGG | 0.370350 | 7.3:-75312603 | None:intergenic |
| CAAAAGAATCTTCACTGCTT+TGG | 0.372561 | 7.3:-75312482 | None:intergenic |
| TTAAACTTTGCATCAGGATA+AGG | 0.382959 | 7.3:-75312406 | None:intergenic |
| CATTAAGACATATTGATTGT+TGG | 0.387585 | 7.3:-75312354 | None:intergenic |
| GCCTGCAACCTTACCAAATC+AGG | 0.389988 | 7.3:-75312526 | None:intergenic |
| GACATATTGATTGTTGGAAT+GGG | 0.405015 | 7.3:-75312348 | None:intergenic |
| TCAGCATCACTTTCTTTGAA+TGG | 0.406038 | 7.3:-75312217 | None:intergenic |
| AGACATATTGATTGTTGGAA+TGG | 0.406677 | 7.3:-75312349 | None:intergenic |
| GAAAGGGAATTTATAACAAA+GGG | 0.412673 | 7.3:+75312623 | MS.gene062430:CDS |
| TCTGCTTTAAACTTTGCATC+AGG | 0.422143 | 7.3:-75312412 | None:intergenic |
| CAAAGACTGTTGGAAAAGGT+TGG | 0.435336 | 7.3:+75312577 | MS.gene062430:CDS |
| TTCTCAAAGACTGTTGGAAA+AGG | 0.441158 | 7.3:+75312573 | MS.gene062430:CDS |
| CATCACTTTCTTTGAATGGC+CGG | 0.455673 | 7.3:-75312213 | None:intergenic |
| TGCTGACGATGTGTTGTTGT+GGG | 0.482447 | 7.3:+75312234 | MS.gene062430:CDS |
| GATTGGAATTATGTAAATCA+AGG | 0.486654 | 7.3:+75312287 | MS.gene062430:CDS |
| AAGACTTTCCTGATTTGGTA+AGG | 0.487902 | 7.3:+75312518 | MS.gene062430:CDS |
| CATGGCTTCTCAAAGACTGT+TGG | 0.500304 | 7.3:+75312567 | MS.gene062430:CDS |
| TTACCAAATCAGGAAAGTCT+TGG | 0.502664 | 7.3:-75312516 | None:intergenic |
| CTGCTTTGGTAGCTACACCT+TGG | 0.510305 | 7.3:-75312468 | None:intergenic |
| CGATGAAATTCCTCATGCTA+AGG | 0.513932 | 7.3:+75312684 | MS.gene062430:CDS |
| GGGTTCAACTATTGGGGCCA+AGG | 0.514130 | 7.3:+75312451 | MS.gene062430:CDS |
| AGAAAGGGAATTTATAACAA+AGG | 0.541022 | 7.3:+75312622 | MS.gene062430:CDS |
| GATGAAATTCCTCATGCTAA+GGG | 0.544681 | 7.3:+75312685 | MS.gene062430:CDS |
| AGTCATGCAATATATGCGAT+TGG | 0.551352 | 7.3:+75312270 | MS.gene062430:CDS |
| TGGAAAAGGTTGGTTTCCAA+AGG | 0.552140 | 7.3:+75312587 | MS.gene062430:CDS |
| AGGGAGGGATTGTTCAGAAA+GGG | 0.555894 | 7.3:+75312607 | MS.gene062430:CDS |
| GGAAAAGGTTGGTTTCCAAA+GGG | 0.561564 | 7.3:+75312588 | MS.gene062430:CDS |
| GCTATTGGGTTCAACTATTG+GGG | 0.562007 | 7.3:+75312445 | MS.gene062430:CDS |
| ATGCTGACGATGTGTTGTTG+TGG | 0.569510 | 7.3:+75312233 | MS.gene062430:CDS |
| AAAGGTTGGTTTCCAAAGGG+AGG | 0.574627 | 7.3:+75312591 | MS.gene062430:CDS |
| TAATACTCGTCCAGAGAACA+TGG | 0.576553 | 7.3:+75312549 | MS.gene062430:CDS |
| ATTGGAATTATGTAAATCAA+GGG | 0.579184 | 7.3:+75312288 | MS.gene062430:CDS |
| AAGAGTCAAGGATCACTCTC+CGG | 0.583353 | 7.3:+75312194 | MS.gene062430:CDS |
| AAGGGAGGGATTGTTCAGAA+AGG | 0.588499 | 7.3:+75312606 | MS.gene062430:CDS |
| AAGGTTGGTTTCCAAAGGGA+GGG | 0.591625 | 7.3:+75312592 | MS.gene062430:CDS |
| ACTAATTAACCCTTAGCATG+AGG | 0.612515 | 7.3:-75312694 | None:intergenic |
| TTATAACAAAGGGAATGTAG+AGG | 0.632962 | 7.3:+75312633 | MS.gene062430:CDS |
| CTTAGCATGAGGAATTTCAT+CGG | 0.640114 | 7.3:-75312683 | None:intergenic |
| TGGCAATAGATCAAGAGTCA+AGG | 0.642231 | 7.3:+75312182 | MS.gene062430:CDS |
| ACTTTGCATCAGGATAAGGA+AGG | 0.674910 | 7.3:-75312402 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGGAATTATGTAAATCAA+GGG | + | chr7.3:75312288-75312307 | MS.gene062430:CDS | 20.0% |
| ! | AGAAAGGGAATTTATAACAA+AGG | + | chr7.3:75312622-75312641 | MS.gene062430:CDS | 25.0% |
| ! | GAAAGGGAATTTATAACAAA+GGG | + | chr7.3:75312623-75312642 | MS.gene062430:CDS | 25.0% |
| ! | GATTGGAATTATGTAAATCA+AGG | + | chr7.3:75312287-75312306 | MS.gene062430:CDS | 25.0% |
| !! | CATTAAGACATATTGATTGT+TGG | - | chr7.3:75312357-75312376 | None:intergenic | 25.0% |
| TGTCTTAATGATCACTCTAT+TGG | + | chr7.3:75312367-75312386 | MS.gene062430:CDS | 30.0% | |
| TTAAACTTTGCATCAGGATA+AGG | - | chr7.3:75312409-75312428 | None:intergenic | 30.0% | |
| TTATAACAAAGGGAATGTAG+AGG | + | chr7.3:75312633-75312652 | MS.gene062430:CDS | 30.0% | |
| ! | AGACATATTGATTGTTGGAA+TGG | - | chr7.3:75312352-75312371 | None:intergenic | 30.0% |
| ! | GACATATTGATTGTTGGAAT+GGG | - | chr7.3:75312351-75312370 | None:intergenic | 30.0% |
| ! | GCAAAGTTTAAAGCAGATTT+AGG | + | chr7.3:75312418-75312437 | MS.gene062430:CDS | 30.0% |
| AGTCATGCAATATATGCGAT+TGG | + | chr7.3:75312270-75312289 | MS.gene062430:CDS | 35.0% | |
| ATGCTATTGGGTTCAACTAT+TGG | + | chr7.3:75312443-75312462 | MS.gene062430:CDS | 35.0% | |
| ATTCCAAGACTTTCCTGATT+TGG | + | chr7.3:75312513-75312532 | MS.gene062430:CDS | 35.0% | |
| CAAAAGAATCTTCACTGCTT+TGG | - | chr7.3:75312485-75312504 | None:intergenic | 35.0% | |
| CAGATTTAGGCTATGCTATT+GGG | + | chr7.3:75312431-75312450 | MS.gene062430:CDS | 35.0% | |
| CTTAGCATGAGGAATTTCAT+CGG | - | chr7.3:75312686-75312705 | None:intergenic | 35.0% | |
| GATGAAATTCCTCATGCTAA+GGG | + | chr7.3:75312685-75312704 | MS.gene062430:CDS | 35.0% | |
| TCAGCATCACTTTCTTTGAA+TGG | - | chr7.3:75312220-75312239 | None:intergenic | 35.0% | |
| TGCTATTGGGTTCAACTATT+GGG | + | chr7.3:75312444-75312463 | MS.gene062430:CDS | 35.0% | |
| TTACCAAATCAGGAAAGTCT+TGG | - | chr7.3:75312519-75312538 | None:intergenic | 35.0% | |
| TTCTCAAAGACTGTTGGAAA+AGG | + | chr7.3:75312573-75312592 | MS.gene062430:CDS | 35.0% | |
| ! | TCTGCTTTAAACTTTGCATC+AGG | - | chr7.3:75312415-75312434 | None:intergenic | 35.0% |
| !! | AAGACTTTCCTGATTTGGTA+AGG | + | chr7.3:75312518-75312537 | MS.gene062430:CDS | 35.0% |
| ACTTTGCATCAGGATAAGGA+AGG | - | chr7.3:75312405-75312424 | None:intergenic | 40.0% | |
| CAAAGACTGTTGGAAAAGGT+TGG | + | chr7.3:75312577-75312596 | MS.gene062430:CDS | 40.0% | |
| CATCACTTTCTTTGAATGGC+CGG | - | chr7.3:75312216-75312235 | None:intergenic | 40.0% | |
| CGATGAAATTCCTCATGCTA+AGG | + | chr7.3:75312684-75312703 | MS.gene062430:CDS | 40.0% | |
| GCAGATTTAGGCTATGCTAT+TGG | + | chr7.3:75312430-75312449 | MS.gene062430:CDS | 40.0% | |
| GCTATTGGGTTCAACTATTG+GGG | + | chr7.3:75312445-75312464 | MS.gene062430:CDS | 40.0% | |
| TAATACTCGTCCAGAGAACA+TGG | + | chr7.3:75312549-75312568 | MS.gene062430:CDS | 40.0% | |
| TGGCAATAGATCAAGAGTCA+AGG | + | chr7.3:75312182-75312201 | MS.gene062430:CDS | 40.0% | |
| !! | GGAAAAGGTTGGTTTCCAAA+GGG | + | chr7.3:75312588-75312607 | MS.gene062430:CDS | 40.0% |
| !! | TGGAAAAGGTTGGTTTCCAA+AGG | + | chr7.3:75312587-75312606 | MS.gene062430:CDS | 40.0% |
| AAGAGTCAAGGATCACTCTC+CGG | + | chr7.3:75312194-75312213 | MS.gene062430:CDS | 45.0% | |
| AAGGGAGGGATTGTTCAGAA+AGG | + | chr7.3:75312606-75312625 | MS.gene062430:CDS | 45.0% | |
| AGGGAGGGATTGTTCAGAAA+GGG | + | chr7.3:75312607-75312626 | MS.gene062430:CDS | 45.0% | |
| CATGGCTTCTCAAAGACTGT+TGG | + | chr7.3:75312567-75312586 | MS.gene062430:CDS | 45.0% | |
| TTCTGAACAATCCCTCCCTT+TGG | - | chr7.3:75312606-75312625 | None:intergenic | 45.0% | |
| ! | ATGCTGACGATGTGTTGTTG+TGG | + | chr7.3:75312233-75312252 | MS.gene062430:CDS | 45.0% |
| ! | CTTTGAGAAGCCATGTTCTC+TGG | - | chr7.3:75312562-75312581 | None:intergenic | 45.0% |
| ! | TGCTGACGATGTGTTGTTGT+GGG | + | chr7.3:75312234-75312253 | MS.gene062430:CDS | 45.0% |
| !! | AAAGGTTGGTTTCCAAAGGG+AGG | + | chr7.3:75312591-75312610 | MS.gene062430:CDS | 45.0% |
| !! | AAGGTTGGTTTCCAAAGGGA+GGG | + | chr7.3:75312592-75312611 | MS.gene062430:CDS | 45.0% |
| !! | TCCTGATTTGGTAAGGTTGC+AGG | + | chr7.3:75312525-75312544 | MS.gene062430:CDS | 45.0% |
| GCCTGCAACCTTACCAAATC+AGG | - | chr7.3:75312529-75312548 | None:intergenic | 50.0% | |
| ! | CTGCTTTGGTAGCTACACCT+TGG | - | chr7.3:75312471-75312490 | None:intergenic | 50.0% |
| GGGTTCAACTATTGGGGCCA+AGG | + | chr7.3:75312451-75312470 | MS.gene062430:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 75312181 | 75312711 | 75312181 | ID=MS.gene062430 |
| chr7.3 | mRNA | 75312181 | 75312711 | 75312181 | ID=MS.gene062430.t1;Parent=MS.gene062430 |
| chr7.3 | exon | 75312181 | 75312711 | 75312181 | ID=MS.gene062430.t1.exon1;Parent=MS.gene062430.t1 |
| chr7.3 | CDS | 75312181 | 75312711 | 75312181 | ID=cds.MS.gene062430.t1;Parent=MS.gene062430.t1 |
| Gene Sequence |
| Protein sequence |
