Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065389.t1 | XP_003624498.1 | 97.9 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 3.20E-73 | 284.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065389.t1 | Q96500 | 49.7 | 143 | 65 | 4 | 1 | 141 | 1 | 138 | 3.1e-24 | 112.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065389.t1 | B7FGR7 | 97.9 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 2.3e-73 | 284.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene065389 | 0.829644 | 4.59E-55 | -1.69E-46 |
| MS.gene050301 | MS.gene065389 | 0.805101 | 1.57E-49 | -1.69E-46 |
| MS.gene050404 | MS.gene065389 | 0.898682 | 4.17E-77 | -1.69E-46 |
| MS.gene050406 | MS.gene065389 | 0.894895 | 1.60E-75 | -1.69E-46 |
| MS.gene050464 | MS.gene065389 | 0.801938 | 7.11E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene065389 | 0.825788 | 3.87E-54 | -1.69E-46 |
| MS.gene050783 | MS.gene065389 | 0.801322 | 9.51E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene065389 | 0.829787 | 4.23E-55 | -1.69E-46 |
| MS.gene051171 | MS.gene065389 | 0.845792 | 3.25E-59 | -1.69E-46 |
| MS.gene051240 | MS.gene065389 | 0.837772 | 4.27E-57 | -1.69E-46 |
| MS.gene051242 | MS.gene065389 | 0.845498 | 3.91E-59 | -1.69E-46 |
| MS.gene051471 | MS.gene065389 | 0.830777 | 2.43E-55 | -1.69E-46 |
| MS.gene052078 | MS.gene065389 | 0.829431 | 5.17E-55 | -1.69E-46 |
| MS.gene052178 | MS.gene065389 | 0.826654 | 2.41E-54 | -1.69E-46 |
| MS.gene052340 | MS.gene065389 | 0.80062 | 1.32E-48 | -1.69E-46 |
| MS.gene053330 | MS.gene065389 | 0.802833 | 4.65E-49 | -1.69E-46 |
| MS.gene053557 | MS.gene065389 | 0.811454 | 6.94E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene065389 | 0.80796 | 3.92E-50 | -1.69E-46 |
| MS.gene053907 | MS.gene065389 | 0.853935 | 1.72E-61 | -1.69E-46 |
| MS.gene053971 | MS.gene065389 | 0.860903 | 1.49E-63 | -1.69E-46 |
| MS.gene055254 | MS.gene065389 | 0.827086 | 1.90E-54 | -1.69E-46 |
| MS.gene055428 | MS.gene065389 | 0.833227 | 6.02E-56 | -1.69E-46 |
| MS.gene055712 | MS.gene065389 | 0.822254 | 2.61E-53 | -1.69E-46 |
| MS.gene055980 | MS.gene065389 | 0.812473 | 4.17E-51 | -1.69E-46 |
| MS.gene056451 | MS.gene065389 | 0.820806 | 5.65E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene065389 | 0.859952 | 2.90E-63 | -1.69E-46 |
| MS.gene056633 | MS.gene065389 | 0.830798 | 2.40E-55 | -1.69E-46 |
| MS.gene056664 | MS.gene065389 | 0.810933 | 9.01E-51 | -1.69E-46 |
| MS.gene056772 | MS.gene065389 | 0.814935 | 1.20E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene065389 | 0.836959 | 6.89E-57 | -1.69E-46 |
| MS.gene057295 | MS.gene065389 | 0.809159 | 2.17E-50 | -1.69E-46 |
| MS.gene057439 | MS.gene065389 | 0.816365 | 5.74E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene065389 | 0.803635 | 3.17E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene065389 | 0.803156 | 3.99E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene065389 | 0.809298 | 2.03E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene065389 | 0.835193 | 1.93E-56 | -1.69E-46 |
| MS.gene058276 | MS.gene065389 | 0.853442 | 2.38E-61 | -1.69E-46 |
| MS.gene058746 | MS.gene065389 | 0.850523 | 1.61E-60 | -1.69E-46 |
| MS.gene059894 | MS.gene065389 | 0.872377 | 3.33E-67 | -1.69E-46 |
| MS.gene059899 | MS.gene065389 | 0.813023 | 3.16E-51 | -1.69E-46 |
| MS.gene060407 | MS.gene065389 | 0.823229 | 1.55E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene065389 | 0.848924 | 4.49E-60 | -1.69E-46 |
| MS.gene061112 | MS.gene065389 | 0.868775 | 5.08E-66 | -1.69E-46 |
| MS.gene061265 | MS.gene065389 | 0.855511 | 6.01E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065389.t1 | MTR_7g084000 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 2.06e-103 | 291 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065389.t1 | AT3G26740 | 48.611 | 144 | 65 | 4 | 1 | 141 | 1 | 138 | 6.75e-33 | 113 |
Find 34 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACTCTTTGGTGTAGTTAA+AGG | 0.261648 | 7.4:-23125208 | None:intergenic |
| GGTGTATCATTCCTTGCTTC+AGG | 0.283991 | 7.4:-23125933 | None:intergenic |
| TCAGGCTGCAGCTTGACTTC+AGG | 0.292025 | 7.4:-23125915 | None:intergenic |
| AGAACGTGAGGCAATCAATT+TGG | 0.330098 | 7.4:-23125263 | None:intergenic |
| TTCCCTGAAGCTGCAGTCTT+TGG | 0.331736 | 7.4:-23125954 | None:intergenic |
| ACACCAAAGACTGCAGCTTC+AGG | 0.337411 | 7.4:+23125951 | MS.gene065389:CDS |
| GGTTGGAGGGGATGCTTGTT+TGG | 0.357304 | 7.4:+23125881 | MS.gene065389:CDS |
| ATAGCGAAATCTTACGTCTT+AGG | 0.366730 | 7.4:-23126008 | None:intergenic |
| GCAAGATGTGAGACACTCTT+TGG | 0.410991 | 7.4:-23125220 | None:intergenic |
| CTGCGAGCCTGATTATCAAA+AGG | 0.412863 | 7.4:+23126168 | MS.gene065389:CDS |
| ACCGTTTCACATGCCTCAGC+TGG | 0.450987 | 7.4:-23125861 | None:intergenic |
| GCAGAGGCTTGTGATGATCT+TGG | 0.462484 | 7.4:+23126136 | MS.gene065389:CDS |
| CACCAAAGACTGCAGCTTCA+GGG | 0.489453 | 7.4:+23125952 | MS.gene065389:CDS |
| TGCGAGCCTGATTATCAAAA+GGG | 0.500804 | 7.4:+23126169 | MS.gene065389:CDS |
| GATCATCACAAGCCTCTGCT+TGG | 0.508891 | 7.4:-23126132 | None:intergenic |
| TGTTGAAGGATCAGAGTTGT+CGG | 0.523519 | 7.4:-23125320 | None:intergenic |
| CAGCTTCAGGGAACATTGAG+AGG | 0.539207 | 7.4:+23125964 | MS.gene065389:CDS |
| CGTGAGGCAATCAATTTGGT+AGG | 0.539328 | 7.4:-23125259 | None:intergenic |
| AGTTAAAGGCACAAAGGTAG+TGG | 0.548726 | 7.4:-23125194 | None:intergenic |
| TCCAGCTGAGGCATGTGAAA+CGG | 0.549406 | 7.4:+23125860 | MS.gene065389:CDS |
| GAATTGTAATCAACTGTTGA+AGG | 0.559497 | 7.4:-23125334 | None:intergenic |
| AGGCATGTGAAACGGTTGGA+GGG | 0.565003 | 7.4:+23125868 | MS.gene065389:CDS |
| GCTGAGGCATGTGAAACGGT+TGG | 0.574341 | 7.4:+23125864 | MS.gene065389:CDS |
| TGTCAGAGTGTTTCCAGCTG+AGG | 0.580476 | 7.4:+23125848 | MS.gene065389:intron |
| CACAAAGGTAGTGGTTGCAA+AGG | 0.582454 | 7.4:-23125185 | None:intergenic |
| GAGGCATGTGAAACGGTTGG+AGG | 0.591598 | 7.4:+23125867 | MS.gene065389:CDS |
| TCAAGCTGCAGCCTGAAGCA+AGG | 0.602199 | 7.4:+23125922 | MS.gene065389:CDS |
| GAGGCTTGTGATGATCTTGG+AGG | 0.607345 | 7.4:+23126139 | MS.gene065389:CDS |
| GGCATGTGAAACGGTTGGAG+GGG | 0.610004 | 7.4:+23125869 | MS.gene065389:CDS |
| GTGAGGCAATCAATTTGGTA+GGG | 0.637953 | 7.4:-23125258 | None:intergenic |
| AGCTTCAGGGAACATTGAGA+GGG | 0.650830 | 7.4:+23125965 | MS.gene065389:CDS |
| TGGTGTAGTTAAAGGCACAA+AGG | 0.651454 | 7.4:-23125200 | None:intergenic |
| GCGAGGTGAAGAAGAACGTG+AGG | 0.676435 | 7.4:-23125275 | None:intergenic |
| ACTTTGATTGCAGTGTAGCG+AGG | 0.698323 | 7.4:-23125292 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTATATTTATAACCTT+AGG | + | chr7.4:23125452-23125471 | MS.gene065389:intron | 10.0% |
| !! | AACTAAACTAAAATGAATTA+AGG | - | chr7.4:23125390-23125409 | None:intergenic | 15.0% |
| !!! | AATTCATTTTAGTTTAGTTT+AGG | + | chr7.4:23125391-23125410 | MS.gene065389:intron | 15.0% |
| !!! | TAGTATATTATTAGTTTCTA+TGG | + | chr7.4:23125522-23125541 | MS.gene065389:intron | 15.0% |
| !! | AATAACACAAACAAATGTTA+TGG | - | chr7.4:23125822-23125841 | None:intergenic | 20.0% |
| !!! | ATCTCTATAAGTTATTCTTT+TGG | + | chr7.4:23125741-23125760 | MS.gene065389:intron | 20.0% |
| !!! | TATTATTAGTTTCTATGGTA+TGG | + | chr7.4:23125527-23125546 | MS.gene065389:intron | 20.0% |
| ! | ATCAGAATTGTGATGTTAAA+TGG | + | chr7.4:23126086-23126105 | MS.gene065389:intron | 25.0% |
| !! | TTTGTTTCAGGACTAAATTT+TGG | + | chr7.4:23125775-23125794 | MS.gene065389:intron | 25.0% |
| !!! | AGTTTAGTTTAGGTTTTCTT+AGG | + | chr7.4:23125401-23125420 | MS.gene065389:intron | 25.0% |
| !!! | GACTTTTAGAATTTTGTTTC+AGG | + | chr7.4:23125763-23125782 | MS.gene065389:intron | 25.0% |
| AGATCGTGCTCATTATTTAT+AGG | + | chr7.4:23125481-23125500 | MS.gene065389:intron | 30.0% | |
| ATTAAGGTGATAGAGAATGA+AGG | - | chr7.4:23125374-23125393 | None:intergenic | 30.0% | |
| GAATTGTAATCAACTGTTGA+AGG | - | chr7.4:23125337-23125356 | None:intergenic | 30.0% | |
| GATTACCATTTGTCACAAAT+AGG | - | chr7.4:23125599-23125618 | None:intergenic | 30.0% | |
| !!! | AATGGATGATGTGTTTTTTC+AGG | + | chr7.4:23126104-23126123 | MS.gene065389:intron | 30.0% |
| !!! | ATGGATGATGTGTTTTTTCA+GGG | + | chr7.4:23126105-23126124 | MS.gene065389:intron | 30.0% |
| ATAGCGAAATCTTACGTCTT+AGG | - | chr7.4:23126011-23126030 | None:intergenic | 35.0% | |
| GCATACCTATTTGTGACAAA+TGG | + | chr7.4:23125591-23125610 | MS.gene065389:intron | 35.0% | |
| ! | ACACTCTTTGGTGTAGTTAA+AGG | - | chr7.4:23125211-23125230 | None:intergenic | 35.0% |
| !! | AATTTTGGAGAGTCACAACA+TGG | + | chr7.4:23125790-23125809 | MS.gene065389:intron | 35.0% |
| !! | TAGACACCCTTTTGATAATC+AGG | - | chr7.4:23126178-23126197 | None:intergenic | 35.0% |
| AGAACGTGAGGCAATCAATT+TGG | - | chr7.4:23125266-23125285 | None:intergenic | 40.0% | |
| GTGAGGCAATCAATTTGGTA+GGG | - | chr7.4:23125261-23125280 | None:intergenic | 40.0% | |
| TGCGAGCCTGATTATCAAAA+GGG | + | chr7.4:23126169-23126188 | MS.gene065389:CDS | 40.0% | |
| TGTTGAAGGATCAGAGTTGT+CGG | - | chr7.4:23125323-23125342 | None:intergenic | 40.0% | |
| ! | AGTTAAAGGCACAAAGGTAG+TGG | - | chr7.4:23125197-23125216 | None:intergenic | 40.0% |
| ! | TGGTGTAGTTAAAGGCACAA+AGG | - | chr7.4:23125203-23125222 | None:intergenic | 40.0% |
| ACTTTGATTGCAGTGTAGCG+AGG | - | chr7.4:23125295-23125314 | None:intergenic | 45.0% | |
| AGCACGATCTGTACTTCCTA+AGG | - | chr7.4:23125471-23125490 | None:intergenic | 45.0% | |
| AGCTTCAGGGAACATTGAGA+GGG | + | chr7.4:23125965-23125984 | MS.gene065389:CDS | 45.0% | |
| CGTGAGGCAATCAATTTGGT+AGG | - | chr7.4:23125262-23125281 | None:intergenic | 45.0% | |
| CTGCGAGCCTGATTATCAAA+AGG | + | chr7.4:23126168-23126187 | MS.gene065389:CDS | 45.0% | |
| GCAAGATGTGAGACACTCTT+TGG | - | chr7.4:23125223-23125242 | None:intergenic | 45.0% | |
| GGTGTATCATTCCTTGCTTC+AGG | - | chr7.4:23125936-23125955 | None:intergenic | 45.0% | |
| ! | CACAAAGGTAGTGGTTGCAA+AGG | - | chr7.4:23125188-23125207 | None:intergenic | 45.0% |
| ! | TTTCAGGGTTTTCCAAGCAG+AGG | + | chr7.4:23126120-23126139 | MS.gene065389:intron | 45.0% |
| ACACCAAAGACTGCAGCTTC+AGG | + | chr7.4:23125951-23125970 | MS.gene065389:CDS | 50.0% | |
| AGGCATGTGAAACGGTTGGA+GGG | + | chr7.4:23125868-23125887 | MS.gene065389:CDS | 50.0% | |
| CACCAAAGACTGCAGCTTCA+GGG | + | chr7.4:23125952-23125971 | MS.gene065389:CDS | 50.0% | |
| CAGCTTCAGGGAACATTGAG+AGG | + | chr7.4:23125964-23125983 | MS.gene065389:CDS | 50.0% | |
| GAGGCTTGTGATGATCTTGG+AGG | + | chr7.4:23126139-23126158 | MS.gene065389:CDS | 50.0% | |
| GATCATCACAAGCCTCTGCT+TGG | - | chr7.4:23126135-23126154 | None:intergenic | 50.0% | |
| GCAGAGGCTTGTGATGATCT+TGG | + | chr7.4:23126136-23126155 | MS.gene065389:CDS | 50.0% | |
| TCCAGCTGAGGCATGTGAAA+CGG | + | chr7.4:23125860-23125879 | MS.gene065389:CDS | 50.0% | |
| TTCCCTGAAGCTGCAGTCTT+TGG | - | chr7.4:23125957-23125976 | None:intergenic | 50.0% | |
| ! | TGTCAGAGTGTTTCCAGCTG+AGG | + | chr7.4:23125848-23125867 | MS.gene065389:intron | 50.0% |
| ACCGTTTCACATGCCTCAGC+TGG | - | chr7.4:23125864-23125883 | None:intergenic | 55.0% | |
| GAGGCATGTGAAACGGTTGG+AGG | + | chr7.4:23125867-23125886 | MS.gene065389:CDS | 55.0% | |
| GCGAGGTGAAGAAGAACGTG+AGG | - | chr7.4:23125278-23125297 | None:intergenic | 55.0% | |
| GCTGAGGCATGTGAAACGGT+TGG | + | chr7.4:23125864-23125883 | MS.gene065389:CDS | 55.0% | |
| GGCATGTGAAACGGTTGGAG+GGG | + | chr7.4:23125869-23125888 | MS.gene065389:CDS | 55.0% | |
| GGTTGGAGGGGATGCTTGTT+TGG | + | chr7.4:23125881-23125900 | MS.gene065389:CDS | 55.0% | |
| TCAAGCTGCAGCCTGAAGCA+AGG | + | chr7.4:23125922-23125941 | MS.gene065389:CDS | 55.0% | |
| TCAGGCTGCAGCTTGACTTC+AGG | - | chr7.4:23125918-23125937 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 23125169 | 23126201 | 23125169 | ID=MS.gene065389 |
| chr7.4 | mRNA | 23125169 | 23126201 | 23125169 | ID=MS.gene065389.t1;Parent=MS.gene065389 |
| chr7.4 | exon | 23125169 | 23125365 | 23125169 | ID=MS.gene065389.t1.exon1;Parent=MS.gene065389.t1 |
| chr7.4 | CDS | 23125169 | 23125365 | 23125169 | ID=cds.MS.gene065389.t1;Parent=MS.gene065389.t1 |
| chr7.4 | exon | 23125854 | 23126015 | 23125854 | ID=MS.gene065389.t1.exon2;Parent=MS.gene065389.t1 |
| chr7.4 | CDS | 23125854 | 23126015 | 23125854 | ID=cds.MS.gene065389.t1;Parent=MS.gene065389.t1 |
| chr7.4 | exon | 23126126 | 23126201 | 23126126 | ID=MS.gene065389.t1.exon3;Parent=MS.gene065389.t1 |
| chr7.4 | CDS | 23126126 | 23126201 | 23126126 | ID=cds.MS.gene065389.t1;Parent=MS.gene065389.t1 |
| Gene Sequence |
| Protein sequence |
