Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066113.t1 | XP_003603541.2 | 90.3 | 124 | 12 | 0 | 1 | 124 | 1 | 124 | 3.80E-54 | 220.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066113.t1 | G7JCD9 | 90.3 | 124 | 12 | 0 | 1 | 124 | 23 | 146 | 2.7e-54 | 220.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene066113 | -0.836331 | 9.96E-57 | -1.69E-46 |
| MS.gene049419 | MS.gene066113 | 0.821005 | 5.08E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene066113 | -0.818019 | 2.44E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene066113 | -0.816883 | 4.39E-52 | -1.69E-46 |
| MS.gene050105 | MS.gene066113 | -0.83586 | 1.31E-56 | -1.69E-46 |
| MS.gene050654 | MS.gene066113 | -0.801036 | 1.09E-48 | -1.69E-46 |
| MS.gene051122 | MS.gene066113 | -0.800863 | 1.18E-48 | -1.69E-46 |
| MS.gene051332 | MS.gene066113 | -0.804654 | 1.95E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene066113 | -0.802153 | 6.42E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene066113 | -0.819759 | 9.81E-53 | -1.69E-46 |
| MS.gene052674 | MS.gene066113 | -0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene052675 | MS.gene066113 | -0.804535 | 2.06E-49 | -1.69E-46 |
| MS.gene052677 | MS.gene066113 | -0.803589 | 3.24E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene066113 | 0.806177 | 9.34E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene066113 | -0.846414 | 2.20E-59 | -1.69E-46 |
| MS.gene054576 | MS.gene066113 | -0.808911 | 2.45E-50 | -1.69E-46 |
| MS.gene055063 | MS.gene066113 | 0.805767 | 1.14E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene066113 | -0.801852 | 7.40E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene066113 | -0.81154 | 6.65E-51 | -1.69E-46 |
| MS.gene056045 | MS.gene066113 | -0.845512 | 3.88E-59 | -1.69E-46 |
| MS.gene056776 | MS.gene066113 | -0.81363 | 2.32E-51 | -1.69E-46 |
| MS.gene056779 | MS.gene066113 | -0.817341 | 3.47E-52 | -1.69E-46 |
| MS.gene057155 | MS.gene066113 | -0.814702 | 1.35E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene066113 | -0.817928 | 2.56E-52 | -1.69E-46 |
| MS.gene057598 | MS.gene066113 | -0.811589 | 6.49E-51 | -1.69E-46 |
| MS.gene058081 | MS.gene066113 | -0.825137 | 5.52E-54 | -1.69E-46 |
| MS.gene058223 | MS.gene066113 | 0.839096 | 1.94E-57 | -1.69E-46 |
| MS.gene059439 | MS.gene066113 | -0.817836 | 2.68E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene066113 | 0.820558 | 6.44E-53 | -1.69E-46 |
| MS.gene060435 | MS.gene066113 | -0.82933 | 5.47E-55 | -1.69E-46 |
| MS.gene060777 | MS.gene066113 | -0.814083 | 1.85E-51 | -1.69E-46 |
| MS.gene060850 | MS.gene066113 | -0.800425 | 1.45E-48 | -1.69E-46 |
| MS.gene060851 | MS.gene066113 | -0.822968 | 1.78E-53 | -1.69E-46 |
| MS.gene060852 | MS.gene066113 | -0.818597 | 1.80E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene066113 | -0.812562 | 3.98E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene066113 | -0.808949 | 2.41E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene066113 | -0.82336 | 1.44E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066113.t1 | MTR_3g108900 | 90.323 | 124 | 12 | 0 | 1 | 124 | 23 | 146 | 4.72e-71 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066113.t1 | AT5G10700 | 49.580 | 119 | 43 | 3 | 6 | 124 | 24 | 125 | 5.16e-33 | 114 |
| MS.gene066113.t1 | AT5G10700 | 67.901 | 81 | 23 | 2 | 45 | 124 | 47 | 125 | 5.91e-33 | 114 |
Find 38 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTGACTCATTGAGTTAA+AGG | 0.322492 | 3.3:-89030481 | None:intergenic |
| CTGATCGATACTTGGCCATT+AGG | 0.378184 | 3.3:+89030594 | MS.gene066113:CDS |
| ACGCTTCTGTTTCCGCGGTT+TGG | 0.395935 | 3.3:+89030640 | MS.gene066113:CDS |
| AGTGGTGGAATCGGTGGAGG+CGG | 0.421097 | 3.3:-89030502 | None:intergenic |
| TGAGTAACCACACTGCCTAA+TGG | 0.453176 | 3.3:-89030609 | None:intergenic |
| GGTGACAGGATCGTCTTTGT+TGG | 0.456796 | 3.3:-89030664 | None:intergenic |
| TGGTCCAATACGTGGTGCTC+CGG | 0.468634 | 3.3:+89030565 | MS.gene066113:CDS |
| GAGAGTGGTGGAATCGGTGG+AGG | 0.477145 | 3.3:-89030505 | None:intergenic |
| TAAAGGTGAGCTGACTCGTC+CGG | 0.477990 | 3.3:-89030464 | None:intergenic |
| CTCCACATCAACTGATTCGC+CGG | 0.486687 | 3.3:+89030527 | MS.gene066113:CDS |
| CACGCCGGAGCACCACGTAT+TGG | 0.503895 | 3.3:-89030569 | None:intergenic |
| GGCGTGATCTGATCGATACT+TGG | 0.507820 | 3.3:+89030586 | MS.gene066113:CDS |
| CTACAATAATCAACGGTGAC+AGG | 0.516772 | 3.3:-89030678 | None:intergenic |
| TGAGAAGGCGAGGTACGAAC+GGG | 0.519329 | 3.3:-89030397 | None:intergenic |
| GTGGTGGAATCGGTGGAGGC+GGG | 0.520690 | 3.3:-89030501 | None:intergenic |
| TTGAGAAGGCGAGGTACGAA+CGG | 0.522363 | 3.3:-89030398 | None:intergenic |
| TGTGGAGAGAGTGGTGGAAT+CGG | 0.534342 | 3.3:-89030511 | None:intergenic |
| AGGCGAGGTACGAACGGGAG+GGG | 0.553596 | 3.3:-89030392 | None:intergenic |
| TTCCGGCGAATCAGTTGATG+TGG | 0.555932 | 3.3:-89030529 | None:intergenic |
| GGGTTGAAGAGACGAAGCGT+AGG | 0.561056 | 3.3:-89030372 | None:intergenic |
| TTAGGCAGTGTGGTTACTCA+AGG | 0.562328 | 3.3:+89030612 | MS.gene066113:CDS |
| GAAGGCGAGGTACGAACGGG+AGG | 0.562660 | 3.3:-89030394 | None:intergenic |
| GGAGAGAGTGGTGGAATCGG+TGG | 0.566146 | 3.3:-89030508 | None:intergenic |
| AAGGCGAGGTACGAACGGGA+GGG | 0.566860 | 3.3:-89030393 | None:intergenic |
| GCCGGAAAACGTCGATGTTG+TGG | 0.569743 | 3.3:+89030545 | MS.gene066113:CDS |
| TGAAGAGACGAAGCGTAGGA+AGG | 0.573707 | 3.3:-89030368 | None:intergenic |
| ACTAAACTTCGTTCTTGAGA+AGG | 0.577942 | 3.3:-89030412 | None:intergenic |
| GAAGAGACGAAGCGTAGGAA+GGG | 0.601157 | 3.3:-89030367 | None:intergenic |
| GTCGGGGCTACAATAATCAA+CGG | 0.604557 | 3.3:-89030685 | None:intergenic |
| ACTTCGTTCTTGAGAAGGCG+AGG | 0.622634 | 3.3:-89030407 | None:intergenic |
| TTGTCACGCTTCTGTTTCCG+CGG | 0.641384 | 3.3:+89030635 | MS.gene066113:CDS |
| ATCAGTTGATGTGGAGAGAG+TGG | 0.646257 | 3.3:-89030520 | None:intergenic |
| CGATGTTGTGGTCCAATACG+TGG | 0.652164 | 3.3:+89030557 | MS.gene066113:CDS |
| GTCTTTGTTGGACCAAACCG+CGG | 0.653891 | 3.3:-89030652 | None:intergenic |
| AGTTGATGTGGAGAGAGTGG+TGG | 0.659985 | 3.3:-89030517 | None:intergenic |
| TACTTGGCCATTAGGCAGTG+TGG | 0.667910 | 3.3:+89030602 | MS.gene066113:CDS |
| GTTTAGTGTCACTTTACACG+CGG | 0.717247 | 3.3:+89030428 | MS.gene066113:CDS |
| AAGTATCGATCAGATCACGC+CGG | 0.740521 | 3.3:-89030584 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTGCATAGAATCAATTTTGT+CGG | - | chr3.3:89030706-89030725 | None:intergenic | 30.0% |
| ! | TGCATAGAATCAATTTTGTC+GGG | - | chr3.3:89030705-89030724 | None:intergenic | 30.0% |
| ACTAAACTTCGTTCTTGAGA+AGG | - | chr3.3:89030415-89030434 | None:intergenic | 35.0% | |
| ! | GCATAGAATCAATTTTGTCG+GGG | - | chr3.3:89030704-89030723 | None:intergenic | 35.0% |
| CTACAATAATCAACGGTGAC+AGG | - | chr3.3:89030681-89030700 | None:intergenic | 40.0% | |
| ! | GGGTTGACTCATTGAGTTAA+AGG | - | chr3.3:89030484-89030503 | None:intergenic | 40.0% |
| !! | GTTTAGTGTCACTTTACACG+CGG | + | chr3.3:89030428-89030447 | MS.gene066113:CDS | 40.0% |
| CTGATCGATACTTGGCCATT+AGG | + | chr3.3:89030594-89030613 | MS.gene066113:CDS | 45.0% | |
| GTCGGGGCTACAATAATCAA+CGG | - | chr3.3:89030688-89030707 | None:intergenic | 45.0% | |
| TGAGTAACCACACTGCCTAA+TGG | - | chr3.3:89030612-89030631 | None:intergenic | 45.0% | |
| TTAGGCAGTGTGGTTACTCA+AGG | + | chr3.3:89030612-89030631 | MS.gene066113:CDS | 45.0% | |
| ! | ACCACAACATCGACGTTTTC+CGG | - | chr3.3:89030549-89030568 | None:intergenic | 45.0% |
| ! | ACTCGTCCGGTTTGAAAAAG+AGG | - | chr3.3:89030454-89030473 | None:intergenic | 45.0% |
| ! | ATCAGTTGATGTGGAGAGAG+TGG | - | chr3.3:89030523-89030542 | None:intergenic | 45.0% |
| !! | AAGTATCGATCAGATCACGC+CGG | - | chr3.3:89030587-89030606 | None:intergenic | 45.0% |
| ACTTCGTTCTTGAGAAGGCG+AGG | - | chr3.3:89030410-89030429 | None:intergenic | 50.0% | |
| CGATGTTGTGGTCCAATACG+TGG | + | chr3.3:89030557-89030576 | MS.gene066113:CDS | 50.0% | |
| GAAGAGACGAAGCGTAGGAA+GGG | - | chr3.3:89030370-89030389 | None:intergenic | 50.0% | |
| GGCGTGATCTGATCGATACT+TGG | + | chr3.3:89030586-89030605 | MS.gene066113:CDS | 50.0% | |
| GGTGACAGGATCGTCTTTGT+TGG | - | chr3.3:89030667-89030686 | None:intergenic | 50.0% | |
| GTCTTTGTTGGACCAAACCG+CGG | - | chr3.3:89030655-89030674 | None:intergenic | 50.0% | |
| TACTTGGCCATTAGGCAGTG+TGG | + | chr3.3:89030602-89030621 | MS.gene066113:CDS | 50.0% | |
| TGAAGAGACGAAGCGTAGGA+AGG | - | chr3.3:89030371-89030390 | None:intergenic | 50.0% | |
| TTGAGAAGGCGAGGTACGAA+CGG | - | chr3.3:89030401-89030420 | None:intergenic | 50.0% | |
| TTGTCACGCTTCTGTTTCCG+CGG | + | chr3.3:89030635-89030654 | MS.gene066113:CDS | 50.0% | |
| ! | AGTTGATGTGGAGAGAGTGG+TGG | - | chr3.3:89030520-89030539 | None:intergenic | 50.0% |
| ! | TGTGGAGAGAGTGGTGGAAT+CGG | - | chr3.3:89030514-89030533 | None:intergenic | 50.0% |
| ! | TTCCGGCGAATCAGTTGATG+TGG | - | chr3.3:89030532-89030551 | None:intergenic | 50.0% |
| !! | CTCCACATCAACTGATTCGC+CGG | + | chr3.3:89030527-89030546 | MS.gene066113:CDS | 50.0% |
| !! | TAAAGGTGAGCTGACTCGTC+CGG | - | chr3.3:89030467-89030486 | None:intergenic | 50.0% |
| !!! | ACGCGGCCTCTTTTTCAAAC+CGG | + | chr3.3:89030445-89030464 | MS.gene066113:CDS | 50.0% |
| ACGCTTCTGTTTCCGCGGTT+TGG | + | chr3.3:89030640-89030659 | MS.gene066113:CDS | 55.0% | |
| GCCGGAAAACGTCGATGTTG+TGG | + | chr3.3:89030545-89030564 | MS.gene066113:CDS | 55.0% | |
| GGGTTGAAGAGACGAAGCGT+AGG | - | chr3.3:89030375-89030394 | None:intergenic | 55.0% | |
| TGAGAAGGCGAGGTACGAAC+GGG | - | chr3.3:89030400-89030419 | None:intergenic | 55.0% | |
| TGGTCCAATACGTGGTGCTC+CGG | + | chr3.3:89030565-89030584 | MS.gene066113:CDS | 55.0% | |
| AAGGCGAGGTACGAACGGGA+GGG | - | chr3.3:89030396-89030415 | None:intergenic | 60.0% | |
| ! | AGTGGTGGAATCGGTGGAGG+CGG | - | chr3.3:89030505-89030524 | None:intergenic | 60.0% |
| ! | GAGAGTGGTGGAATCGGTGG+AGG | - | chr3.3:89030508-89030527 | None:intergenic | 60.0% |
| ! | GGAGAGAGTGGTGGAATCGG+TGG | - | chr3.3:89030511-89030530 | None:intergenic | 60.0% |
| AGGCGAGGTACGAACGGGAG+GGG | - | chr3.3:89030395-89030414 | None:intergenic | 65.0% | |
| GAAGGCGAGGTACGAACGGG+AGG | - | chr3.3:89030397-89030416 | None:intergenic | 65.0% | |
| ! | CACGCCGGAGCACCACGTAT+TGG | - | chr3.3:89030572-89030591 | None:intergenic | 65.0% |
| ! | GTGGTGGAATCGGTGGAGGC+GGG | - | chr3.3:89030504-89030523 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 89030357 | 89030728 | 89030357 | ID=MS.gene066113 |
| chr3.3 | mRNA | 89030357 | 89030728 | 89030357 | ID=MS.gene066113.t1;Parent=MS.gene066113 |
| chr3.3 | exon | 89030357 | 89030728 | 89030357 | ID=MS.gene066113.t1.exon1;Parent=MS.gene066113.t1 |
| chr3.3 | CDS | 89030357 | 89030728 | 89030357 | ID=cds.MS.gene066113.t1;Parent=MS.gene066113.t1 |
| Gene Sequence |
| Protein sequence |
