Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066538.t1 | KAB1202362.1 | 65.3 | 714 | 172 | 7 | 16 | 719 | 15 | 662 | 1.40E-252 | 882.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066538.t1 | Q3EBC2 | 61.4 | 368 | 133 | 2 | 348 | 709 | 6 | 370 | 6.4e-126 | 453.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066538.t1 | G7KZR9 | 98.9 | 363 | 4 | 0 | 347 | 709 | 5 | 367 | 6.2e-207 | 730.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049609 | MS.gene066538 | 0.809232 | 2.10E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene066538 | 0.865877 | 4.29E-65 | -1.69E-46 |
| MS.gene050670 | MS.gene066538 | 0.868202 | 7.78E-66 | -1.69E-46 |
| MS.gene05105 | MS.gene066538 | 0.809729 | 1.64E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene066538 | 0.857042 | 2.14E-62 | -1.69E-46 |
| MS.gene051521 | MS.gene066538 | 0.817512 | 3.17E-52 | -1.69E-46 |
| MS.gene051525 | MS.gene066538 | 0.83541 | 1.71E-56 | -1.69E-46 |
| MS.gene051582 | MS.gene066538 | 0.813799 | 2.13E-51 | -1.69E-46 |
| MS.gene051588 | MS.gene066538 | 0.82624 | 3.02E-54 | -1.69E-46 |
| MS.gene051838 | MS.gene066538 | 0.807549 | 4.79E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene066538 | 0.832493 | 9.16E-56 | -1.69E-46 |
| MS.gene052501 | MS.gene066538 | 0.804005 | 2.66E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene066538 | 0.822908 | 1.84E-53 | -1.69E-46 |
| MS.gene052982 | MS.gene066538 | 0.82398 | 1.03E-53 | -1.69E-46 |
| MS.gene053169 | MS.gene066538 | 0.829056 | 6.37E-55 | -1.69E-46 |
| MS.gene053207 | MS.gene066538 | 0.819079 | 1.40E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene066538 | 0.806814 | 6.85E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene066538 | 0.821235 | 4.50E-53 | -1.69E-46 |
| MS.gene054927 | MS.gene066538 | 0.810415 | 1.17E-50 | -1.69E-46 |
| MS.gene055369 | MS.gene066538 | 0.87047 | 1.42E-66 | -1.69E-46 |
| MS.gene05553 | MS.gene066538 | 0.813344 | 2.68E-51 | -1.69E-46 |
| MS.gene055564 | MS.gene066538 | 0.832757 | 7.88E-56 | -1.69E-46 |
| MS.gene055744 | MS.gene066538 | 0.803745 | 3.01E-49 | -1.69E-46 |
| MS.gene055909 | MS.gene066538 | 0.805058 | 1.60E-49 | -1.69E-46 |
| MS.gene055911 | MS.gene066538 | 0.812555 | 4.00E-51 | -1.69E-46 |
| MS.gene05594 | MS.gene066538 | 0.831924 | 1.27E-55 | -1.69E-46 |
| MS.gene056407 | MS.gene066538 | 0.845269 | 4.51E-59 | -1.69E-46 |
| MS.gene056422 | MS.gene066538 | 0.811103 | 8.28E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene066538 | 0.841446 | 4.73E-58 | -1.69E-46 |
| MS.gene056514 | MS.gene066538 | 0.818004 | 2.46E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene066538 | 0.803792 | 2.94E-49 | -1.69E-46 |
| MS.gene056877 | MS.gene066538 | 0.837671 | 4.53E-57 | -1.69E-46 |
| MS.gene057124 | MS.gene066538 | 0.835876 | 1.30E-56 | -1.69E-46 |
| MS.gene057331 | MS.gene066538 | 0.805221 | 1.48E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene066538 | 0.80512 | 1.56E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene066538 | 0.817883 | 2.62E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene066538 | 0.823189 | 1.58E-53 | -1.69E-46 |
| MS.gene057856 | MS.gene066538 | 0.812596 | 3.91E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene066538 | 0.804088 | 2.55E-49 | -1.69E-46 |
| MS.gene058700 | MS.gene066538 | 0.842044 | 3.29E-58 | -1.69E-46 |
| MS.gene058948 | MS.gene066538 | 0.803983 | 2.69E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene066538 | 0.84365 | 1.23E-58 | -1.69E-46 |
| MS.gene059676 | MS.gene066538 | 0.803576 | 3.26E-49 | -1.69E-46 |
| MS.gene059933 | MS.gene066538 | 0.835014 | 2.15E-56 | -1.69E-46 |
| MS.gene060403 | MS.gene066538 | 0.841743 | 3.95E-58 | -1.69E-46 |
| MS.gene060835 | MS.gene066538 | 0.8548 | 9.67E-62 | -1.69E-46 |
| MS.gene060837 | MS.gene066538 | 0.826778 | 2.25E-54 | -1.69E-46 |
| MS.gene06092 | MS.gene066538 | 0.80358 | 3.26E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene066538 | 0.856621 | 2.84E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066538.t1 | MTR_7g114080 | 98.626 | 364 | 5 | 0 | 347 | 710 | 5 | 368 | 0.0 | 752 |
| MS.gene066538.t1 | MTR_7g114080 | 96.154 | 364 | 5 | 1 | 347 | 710 | 5 | 359 | 0.0 | 725 |
| MS.gene066538.t1 | MTR_7g114080 | 99.327 | 297 | 2 | 0 | 347 | 643 | 5 | 301 | 0.0 | 617 |
| MS.gene066538.t1 | MTR_7g114070 | 98.693 | 306 | 3 | 1 | 1 | 305 | 7 | 312 | 0.0 | 609 |
| MS.gene066538.t1 | MTR_7g114080 | 99.216 | 255 | 2 | 0 | 347 | 601 | 5 | 259 | 0.0 | 529 |
| MS.gene066538.t1 | MTR_7g114070 | 97.817 | 229 | 4 | 1 | 1 | 228 | 7 | 235 | 4.41e-155 | 450 |
| MS.gene066538.t1 | MTR_7g114070 | 97.817 | 229 | 4 | 1 | 1 | 228 | 7 | 235 | 5.66e-155 | 450 |
| MS.gene066538.t1 | MTR_7g114070 | 98.246 | 228 | 3 | 1 | 1 | 227 | 7 | 234 | 1.90e-153 | 448 |
| MS.gene066538.t1 | MTR_7g088350 | 34.694 | 147 | 70 | 3 | 502 | 648 | 163 | 283 | 1.20e-20 | 96.3 |
| MS.gene066538.t1 | MTR_6g033315 | 34.014 | 147 | 77 | 2 | 502 | 648 | 150 | 276 | 5.87e-20 | 93.6 |
| MS.gene066538.t1 | MTR_3g098840 | 28.205 | 195 | 113 | 3 | 502 | 696 | 151 | 318 | 1.15e-19 | 92.8 |
| MS.gene066538.t1 | MTR_6g038020 | 29.767 | 215 | 103 | 5 | 460 | 648 | 71 | 263 | 2.46e-19 | 91.7 |
| MS.gene066538.t1 | MTR_6g038020 | 29.767 | 215 | 103 | 5 | 460 | 648 | 74 | 266 | 3.73e-19 | 91.3 |
| MS.gene066538.t1 | MTR_1g112830 | 39.167 | 120 | 66 | 2 | 462 | 578 | 418 | 533 | 6.31e-19 | 91.7 |
| MS.gene066538.t1 | MTR_1g098390 | 33.333 | 147 | 79 | 2 | 502 | 648 | 136 | 263 | 3.08e-18 | 88.2 |
| MS.gene066538.t1 | MTR_4g116160 | 41.748 | 103 | 47 | 2 | 462 | 552 | 325 | 426 | 1.05e-17 | 87.4 |
| MS.gene066538.t1 | MTR_4g116160 | 41.748 | 103 | 47 | 2 | 462 | 552 | 152 | 253 | 1.32e-17 | 86.7 |
| MS.gene066538.t1 | MTR_4g116160 | 41.748 | 103 | 47 | 2 | 462 | 552 | 325 | 426 | 1.55e-17 | 87.4 |
| MS.gene066538.t1 | MTR_7g083480 | 28.505 | 214 | 114 | 5 | 504 | 711 | 152 | 332 | 2.00e-17 | 85.9 |
| MS.gene066538.t1 | MTR_4g092760 | 35.714 | 140 | 77 | 3 | 425 | 551 | 184 | 323 | 5.12e-17 | 84.7 |
| MS.gene066538.t1 | MTR_4g092760 | 36.170 | 141 | 76 | 4 | 425 | 551 | 272 | 412 | 1.15e-16 | 84.3 |
| MS.gene066538.t1 | MTR_2g066060 | 29.644 | 253 | 164 | 6 | 53 | 303 | 123 | 363 | 3.15e-16 | 81.6 |
| MS.gene066538.t1 | MTR_7g088360 | 28.191 | 188 | 105 | 3 | 502 | 688 | 162 | 320 | 5.60e-16 | 81.3 |
| MS.gene066538.t1 | MTR_1g094610 | 33.858 | 127 | 78 | 3 | 462 | 582 | 55 | 181 | 6.62e-16 | 79.0 |
| MS.gene066538.t1 | MTR_4g021795 | 30.323 | 155 | 83 | 4 | 428 | 565 | 23 | 169 | 2.47e-15 | 77.8 |
| MS.gene066538.t1 | MTR_5g023230 | 38.554 | 83 | 43 | 2 | 504 | 586 | 170 | 244 | 2.26e-13 | 70.9 |
| MS.gene066538.t1 | MTR_5g023230 | 31.429 | 140 | 69 | 3 | 504 | 643 | 150 | 262 | 5.32e-13 | 71.2 |
| MS.gene066538.t1 | MTR_5g023230 | 31.429 | 140 | 69 | 3 | 504 | 643 | 170 | 282 | 6.70e-13 | 71.2 |
| MS.gene066538.t1 | MTR_5g042870 | 59.091 | 44 | 18 | 0 | 504 | 547 | 181 | 224 | 3.73e-12 | 70.1 |
| MS.gene066538.t1 | MTR_4g082560 | 50.000 | 54 | 27 | 0 | 504 | 557 | 181 | 234 | 3.92e-12 | 70.1 |
| MS.gene066538.t1 | MTR_7g118050 | 33.333 | 93 | 46 | 2 | 469 | 545 | 76 | 168 | 4.87e-12 | 66.6 |
| MS.gene066538.t1 | MTR_1g085980 | 37.255 | 102 | 49 | 4 | 469 | 559 | 114 | 211 | 5.31e-12 | 69.3 |
| MS.gene066538.t1 | MTR_7g118050 | 33.333 | 93 | 46 | 2 | 469 | 545 | 76 | 168 | 9.17e-12 | 67.0 |
| MS.gene066538.t1 | MTR_4g074600 | 30.233 | 129 | 81 | 3 | 504 | 625 | 76 | 202 | 3.42e-11 | 65.1 |
| MS.gene066538.t1 | MTR_4g074600 | 31.008 | 129 | 80 | 3 | 504 | 625 | 172 | 298 | 7.81e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066538.t1 | AT3G04970 | 65.489 | 368 | 118 | 2 | 348 | 709 | 6 | 370 | 3.88e-178 | 514 |
| MS.gene066538.t1 | AT3G04970 | 75.676 | 296 | 72 | 0 | 348 | 643 | 6 | 301 | 8.80e-171 | 492 |
| MS.gene066538.t1 | AT5G27290 | 77.032 | 283 | 64 | 1 | 24 | 305 | 31 | 313 | 1.35e-156 | 457 |
| MS.gene066538.t1 | AT3G04970 | 63.251 | 283 | 95 | 2 | 433 | 709 | 4 | 283 | 5.19e-129 | 385 |
| MS.gene066538.t1 | AT5G27290 | 75.862 | 232 | 55 | 1 | 24 | 254 | 31 | 262 | 1.80e-123 | 369 |
| MS.gene066538.t1 | AT1G54680 | 31.633 | 294 | 144 | 4 | 20 | 304 | 9 | 254 | 5.26e-38 | 143 |
| MS.gene066538.t1 | AT1G54680 | 31.333 | 300 | 143 | 4 | 20 | 304 | 9 | 260 | 1.89e-37 | 142 |
| MS.gene066538.t1 | AT1G54680 | 31.818 | 286 | 132 | 4 | 34 | 304 | 12 | 249 | 5.25e-37 | 140 |
| MS.gene066538.t1 | AT1G54680 | 42.446 | 139 | 70 | 2 | 175 | 304 | 51 | 188 | 2.02e-32 | 125 |
| MS.gene066538.t1 | AT5G50020 | 29.057 | 265 | 135 | 6 | 460 | 700 | 72 | 307 | 1.26e-21 | 98.6 |
| MS.gene066538.t1 | AT5G50020 | 29.057 | 265 | 135 | 6 | 460 | 700 | 102 | 337 | 2.24e-21 | 98.2 |
| MS.gene066538.t1 | AT5G41060 | 29.703 | 202 | 102 | 4 | 466 | 647 | 24 | 205 | 3.79e-20 | 93.2 |
| MS.gene066538.t1 | AT5G41060 | 29.703 | 202 | 102 | 4 | 466 | 647 | 24 | 205 | 3.79e-20 | 93.2 |
| MS.gene066538.t1 | AT3G26935 | 28.287 | 251 | 131 | 6 | 466 | 694 | 91 | 314 | 5.26e-20 | 94.0 |
| MS.gene066538.t1 | AT5G41060 | 29.703 | 202 | 102 | 4 | 466 | 647 | 91 | 272 | 6.60e-20 | 93.2 |
| MS.gene066538.t1 | AT5G41060 | 29.557 | 203 | 103 | 4 | 466 | 648 | 76 | 258 | 6.82e-20 | 93.2 |
| MS.gene066538.t1 | AT2G14255 | 42.857 | 98 | 46 | 2 | 465 | 552 | 316 | 413 | 1.24e-19 | 93.6 |
| MS.gene066538.t1 | AT4G24630 | 34.694 | 147 | 76 | 2 | 502 | 648 | 136 | 262 | 3.54e-19 | 90.9 |
| MS.gene066538.t1 | AT3G56930 | 30.415 | 217 | 121 | 4 | 502 | 718 | 146 | 332 | 4.31e-19 | 91.7 |
| MS.gene066538.t1 | AT3G48760 | 30.180 | 222 | 108 | 5 | 454 | 648 | 83 | 284 | 1.68e-18 | 89.7 |
| MS.gene066538.t1 | AT1G56180 | 31.472 | 197 | 121 | 5 | 110 | 303 | 175 | 360 | 9.55e-18 | 86.7 |
| MS.gene066538.t1 | AT5G50020 | 27.925 | 265 | 131 | 7 | 460 | 700 | 72 | 300 | 2.74e-17 | 85.1 |
| MS.gene066538.t1 | AT5G05070 | 31.757 | 148 | 75 | 2 | 502 | 648 | 171 | 293 | 1.01e-16 | 83.6 |
| MS.gene066538.t1 | AT3G56920 | 34.694 | 147 | 72 | 3 | 502 | 648 | 142 | 264 | 1.21e-16 | 82.4 |
| MS.gene066538.t1 | AT2G40990 | 33.333 | 147 | 75 | 3 | 502 | 648 | 160 | 283 | 5.08e-16 | 81.3 |
| MS.gene066538.t1 | AT5G20350 | 36.800 | 125 | 58 | 3 | 459 | 571 | 317 | 432 | 2.82e-15 | 80.1 |
| MS.gene066538.t1 | AT3G09320 | 34.028 | 144 | 69 | 4 | 502 | 643 | 97 | 216 | 6.88e-14 | 73.2 |
| MS.gene066538.t1 | AT1G69420 | 50.000 | 68 | 32 | 2 | 504 | 569 | 165 | 232 | 1.33e-13 | 74.7 |
| MS.gene066538.t1 | AT1G69420 | 50.000 | 68 | 32 | 2 | 504 | 569 | 165 | 232 | 1.33e-13 | 74.7 |
| MS.gene066538.t1 | AT5G04270 | 33.333 | 90 | 60 | 0 | 476 | 565 | 66 | 155 | 3.14e-13 | 71.2 |
| MS.gene066538.t1 | AT4G00840 | 38.824 | 85 | 43 | 3 | 469 | 545 | 71 | 154 | 8.53e-13 | 68.9 |
| MS.gene066538.t1 | AT4G00840 | 38.824 | 85 | 43 | 3 | 469 | 545 | 71 | 154 | 3.50e-12 | 68.2 |
| MS.gene066538.t1 | AT3G51390 | 40.984 | 61 | 36 | 0 | 504 | 564 | 159 | 219 | 1.86e-11 | 66.6 |
| MS.gene066538.t1 | AT3G51390 | 40.984 | 61 | 36 | 0 | 504 | 564 | 164 | 224 | 1.88e-11 | 66.6 |
| MS.gene066538.t1 | AT4G01730 | 28.977 | 176 | 104 | 7 | 469 | 634 | 116 | 280 | 3.74e-11 | 66.6 |
Find 166 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAATTGAAAATTCATTT+TGG | 0.153553 | 7.1:-2464414 | MS.gene066538:CDS |
| CTTAACTTTGCAGTTTATTA+TGG | 0.173681 | 7.1:-2464433 | MS.gene066538:intron |
| AAGAATAATGTTTACAATAA+AGG | 0.202965 | 7.1:-2463301 | MS.gene066538:intron |
| TATTATCATGCAGTGGTTAT+TGG | 0.211095 | 7.1:-2464284 | MS.gene066538:intron |
| ATGAAAGTGCCTTCGAAAAT+TGG | 0.220315 | 7.1:+2468477 | None:intergenic |
| AGGATATACACTTTCTAGTT+TGG | 0.265974 | 7.1:-2471159 | MS.gene066538:CDS |
| GAAAATTAAACCTACTTTGC+AGG | 0.266567 | 7.1:+2470018 | None:intergenic |
| GTTGCTGCTTGGAATGTCTT+TGG | 0.278212 | 7.1:-2472207 | MS.gene066538:CDS |
| TCGTCCCAATCCTCTCTTAC+AGG | 0.291503 | 7.1:-2468249 | MS.gene066538:intron |
| TTGACCAGCTTTACTGATCC+TGG | 0.302736 | 7.1:-2466837 | MS.gene066538:CDS |
| ATTCTCACCCTTGCAAGTTT+AGG | 0.304336 | 7.1:+2472662 | None:intergenic |
| GATCCTTTATGAAGAGAAAA+AGG | 0.309444 | 7.1:-2470055 | MS.gene066538:CDS |
| ATTATCATGCAGTGGTTATT+GGG | 0.310919 | 7.1:-2464283 | MS.gene066538:intron |
| CTAGAAAGTGTATATCCTTT+AGG | 0.318332 | 7.1:+2471164 | None:intergenic |
| AAAGTTGTCTATATTCTAAC+AGG | 0.323299 | 7.1:-2464536 | MS.gene066538:intron |
| GTTTCTGTGGACTCTGGATT+TGG | 0.325214 | 7.1:-2472142 | MS.gene066538:intron |
| TTGGACGAATTGAAGCTAAA+TGG | 0.341057 | 7.1:-2472318 | MS.gene066538:CDS |
| TGCACCGAGTATCTTATATA+TGG | 0.347416 | 7.1:-2470557 | MS.gene066538:CDS |
| TATGTAAAAGATTTGTTGTT+GGG | 0.350037 | 7.1:+2472179 | None:intergenic |
| AGCTGCTCTTGCACTTGTTA+AGG | 0.362242 | 7.1:-2472558 | MS.gene066538:CDS |
| AAGAAGAATCTGAGTGTTGT+AGG | 0.368816 | 7.1:+2464258 | None:intergenic |
| TCAGTCAGAAATACCACAAC+AGG | 0.372976 | 7.1:-2471331 | MS.gene066538:CDS |
| CCCAACGGGCTTCAGTGTTT+CGG | 0.378346 | 7.1:-2472521 | MS.gene066538:CDS |
| CTATTAAAGGTCTTGGTAGC+TGG | 0.380467 | 7.1:-2463167 | MS.gene066538:intron |
| TAAATAGTATCCAGGGATAT+AGG | 0.387946 | 7.1:+2467009 | None:intergenic |
| ATTCACTGTTTCCTCACTTT+CGG | 0.391418 | 7.1:-2468447 | MS.gene066538:CDS |
| GCAATTGTTGGGATGCTGTT+AGG | 0.392269 | 7.1:-2464223 | MS.gene066538:CDS |
| TCAAGCTGGCACAGCCTTTG+TGG | 0.393475 | 7.1:-2471105 | MS.gene066538:CDS |
| CATTTCCTTCTATGTATGTA+TGG | 0.394359 | 7.1:-2464605 | MS.gene066538:CDS |
| CCATGGATTGGTGACAGCTT+TGG | 0.396604 | 7.1:-2468529 | MS.gene066538:CDS |
| TACTGATCCTGGGACAATAA+AGG | 0.405997 | 7.1:-2466826 | MS.gene066538:CDS |
| TGGTGGAGGTCTTGGTAGCT+TGG | 0.407245 | 7.1:-2471378 | MS.gene066538:CDS |
| TAGTTGCACTTAAATTTGAA+TGG | 0.407365 | 7.1:-2463143 | MS.gene066538:CDS |
| AGATTCTTTGCTCAACGGAC+TGG | 0.409538 | 7.1:-2470414 | MS.gene066538:CDS |
| GAGAAACACTCGATATTTCA+TGG | 0.410575 | 7.1:-2466440 | MS.gene066538:CDS |
| CATGTAGTCTTGCCACTTGA+AGG | 0.417053 | 7.1:+2463474 | None:intergenic |
| ATTGCTGTTTCTGTGGACTC+TGG | 0.428658 | 7.1:-2472148 | MS.gene066538:CDS |
| TTCTCAGCCTTTATTGTCCC+AGG | 0.429320 | 7.1:+2466819 | None:intergenic |
| GTGTTTCGGCGCGGCTAGAC+AGG | 0.430642 | 7.1:-2472507 | MS.gene066538:intron |
| TCCAATGTATAAAGCCTTTG+AGG | 0.434942 | 7.1:+2472336 | None:intergenic |
| TTCAGATCTATAACGGAAGT+TGG | 0.436990 | 7.1:-2470228 | MS.gene066538:intron |
| GCATGGTGTCTGTGTGGGCG+TGG | 0.440088 | 7.1:-2472708 | MS.gene066538:CDS |
| AGTTGGATATACTGTGTTCC+TGG | 0.442492 | 7.1:-2470211 | MS.gene066538:CDS |
| AGCTTCTTCGCAGCTGCTGT+TGG | 0.443853 | 7.1:-2466876 | MS.gene066538:CDS |
| GATTCTTTGCTCAACGGACT+GGG | 0.445879 | 7.1:-2470413 | MS.gene066538:CDS |
| ATGTATGGAACAGTTCTCAT+AGG | 0.449403 | 7.1:-2464590 | MS.gene066538:CDS |
| TGGTGTCATGCAGGTTAACT+CGG | 0.450519 | 7.1:-2470753 | MS.gene066538:intron |
| TCTTCCTTTGCCTATATCCC+TGG | 0.452747 | 7.1:-2467019 | MS.gene066538:CDS |
| CCGAAACACTGAAGCCCGTT+GGG | 0.454050 | 7.1:+2472521 | None:intergenic |
| GTGGGTCTCCCATCATCCCT+CGG | 0.454251 | 7.1:-2472689 | MS.gene066538:CDS |
| ATGCTGTTAGGCGGATTCTT+CGG | 0.458907 | 7.1:-2464211 | MS.gene066538:CDS |
| CTTCAAGTGGCAAGACTACA+TGG | 0.460027 | 7.1:-2463473 | MS.gene066538:CDS |
| TGTAGAAAATACCTCATTAG+TGG | 0.460618 | 7.1:+2464156 | None:intergenic |
| TATACTGTGTTCCTGGCTCC+TGG | 0.462759 | 7.1:-2470204 | MS.gene066538:intron |
| GAAACCTGTAAGAGAGGATT+GGG | 0.467880 | 7.1:+2468245 | None:intergenic |
| TTTAGCTCCCCATGTTGTAC+AGG | 0.468240 | 7.1:-2464389 | MS.gene066538:intron |
| TTAATTATGCAGCATGAAGC+TGG | 0.468510 | 7.1:-2471221 | MS.gene066538:intron |
| TAGTTTGGATGGTCTGATGA+AGG | 0.468887 | 7.1:-2471144 | MS.gene066538:CDS |
| TCTCCTGTGGATACAACTCT+TGG | 0.469950 | 7.1:-2472273 | MS.gene066538:CDS |
| TTGTTGGAGGGGTTGCTGCT+TGG | 0.473306 | 7.1:-2472218 | MS.gene066538:CDS |
| AGCTTGGTTGTTGATACAAT+TGG | 0.475036 | 7.1:-2471362 | MS.gene066538:CDS |
| GTTGTGATCTCTTTCCTTTG+CGG | 0.475078 | 7.1:-2468507 | MS.gene066538:CDS |
| AGTTGCACTTAAATTTGAAT+GGG | 0.475690 | 7.1:-2463142 | MS.gene066538:CDS |
| TTGATTGCTGCTATTGTTGG+AGG | 0.477423 | 7.1:-2472231 | MS.gene066538:CDS |
| TGAAACCTGTAAGAGAGGAT+TGG | 0.477635 | 7.1:+2468244 | None:intergenic |
| TTGTTGATTGCTGCTATTGT+TGG | 0.478556 | 7.1:-2472234 | MS.gene066538:CDS |
| ACTTGCAGAATAATTGCATA+GGG | 0.478657 | 7.1:-2466465 | MS.gene066538:intron |
| TTCCGAGACTTCGTGTGTGA+AGG | 0.478726 | 7.1:-2470077 | MS.gene066538:CDS |
| ATTTACAAGGCAAACCCAAC+GGG | 0.479735 | 7.1:-2472535 | MS.gene066538:CDS |
| AATTGTTGTCAAGCAACAAA+TGG | 0.480254 | 7.1:-2463369 | MS.gene066538:CDS |
| GTTATTGGTATGCCATGGAT+TGG | 0.482314 | 7.1:-2468541 | MS.gene066538:CDS |
| TATACACTTTCTAGTTTGGA+TGG | 0.483671 | 7.1:-2471155 | MS.gene066538:CDS |
| CTGTTCCATACATACATAGA+AGG | 0.484200 | 7.1:+2464600 | None:intergenic |
| ATATGTAAAAGATTTGTTGT+TGG | 0.485032 | 7.1:+2472178 | None:intergenic |
| GTAGACAAGGAGCTAACAAA+AGG | 0.485325 | 7.1:-2472590 | MS.gene066538:CDS |
| GTTGCACTTAAATAGTATCC+AGG | 0.487943 | 7.1:+2467001 | None:intergenic |
| GGTTTGTTATTGGTATGCCA+TGG | 0.488441 | 7.1:-2468546 | MS.gene066538:CDS |
| CTTTGGGATTGCTGTTTCTG+TGG | 0.491836 | 7.1:-2472155 | MS.gene066538:CDS |
| CATTAGTGGTAGTGTTACTA+AGG | 0.495405 | 7.1:+2464170 | None:intergenic |
| TCCTCAAAGGCTTTATACAT+TGG | 0.495811 | 7.1:-2472337 | MS.gene066538:CDS |
| AAAATTAAACCTACTTTGCA+GGG | 0.497488 | 7.1:+2470019 | None:intergenic |
| TTAGGTTGCCCGAGGGATGA+TGG | 0.499093 | 7.1:+2472680 | None:intergenic |
| CTGGTTGGAATACTTCCTAA+AGG | 0.508155 | 7.1:-2471179 | MS.gene066538:CDS |
| TGCGTCGGCATGAAGCGGCT+CGG | 0.517038 | 7.1:-2470328 | MS.gene066538:intron |
| TTTCATGTATAGCCTTGGCC+GGG | 0.517216 | 7.1:-2470583 | MS.gene066538:CDS |
| TGACCAGCTTTACTGATCCT+GGG | 0.517218 | 7.1:-2466836 | MS.gene066538:CDS |
| CTGCAATGCTTTGATCTAGC+AGG | 0.517826 | 7.1:+2466636 | None:intergenic |
| TTGTCTGCTGCTAGAAACCT+TGG | 0.520128 | 7.1:+2472628 | None:intergenic |
| AGGCAGATTCTCAAGTTAGA+TGG | 0.520302 | 7.1:-2470376 | MS.gene066538:CDS |
| CATGGTGTCTGTGTGGGCGT+GGG | 0.521142 | 7.1:-2472707 | MS.gene066538:CDS |
| GAGAAAGTTAAAAGAAGCGC+AGG | 0.522389 | 7.1:-2463443 | MS.gene066538:CDS |
| GGTGTCATGCAGGTTAACTC+GGG | 0.525542 | 7.1:-2470752 | MS.gene066538:intron |
| AAGATCACCTCTGGAGGATG+TGG | 0.526947 | 7.1:-2463332 | MS.gene066538:CDS |
| TAGAAACCTTGGTTGAGCAT+TGG | 0.528127 | 7.1:+2472639 | None:intergenic |
| TCTCTTTCCTTTGCGGTCGG+TGG | 0.528235 | 7.1:-2468500 | MS.gene066538:CDS |
| TCAATGTTTCAGATCTATAA+CGG | 0.529311 | 7.1:-2470235 | MS.gene066538:intron |
| TGATTGCTGCTATTGTTGGA+GGG | 0.533663 | 7.1:-2472230 | MS.gene066538:CDS |
| ACTGGGCTTCACACAGAAGA+AGG | 0.533968 | 7.1:-2470396 | MS.gene066538:CDS |
| TAGGTTGCCCGAGGGATGAT+GGG | 0.534094 | 7.1:+2472681 | None:intergenic |
| TAGACAAGGAGCTAACAAAA+GGG | 0.534186 | 7.1:-2472589 | MS.gene066538:CDS |
| ATCCTTCACACACGAAGTCT+CGG | 0.534600 | 7.1:+2470075 | None:intergenic |
| TGATCTCTTTCCTTTGCGGT+CGG | 0.537219 | 7.1:-2468503 | MS.gene066538:CDS |
| AAGAACAGCATTGGTACACT+TGG | 0.540140 | 7.1:+2468295 | None:intergenic |
| CTCATAGGTTTAATTCTCGC+AGG | 0.542159 | 7.1:-2464575 | MS.gene066538:CDS |
| GATTGCTGCTATTGTTGGAG+GGG | 0.542235 | 7.1:-2472229 | MS.gene066538:CDS |
| TGGGTCTCCCATCATCCCTC+GGG | 0.547419 | 7.1:-2472688 | MS.gene066538:CDS |
| GCACTTGTTAAGGATTTACA+AGG | 0.547995 | 7.1:-2472548 | MS.gene066538:CDS |
| TTCAACAGAAAGAACAGCAT+TGG | 0.550937 | 7.1:+2468286 | None:intergenic |
| AATCGTAAGCGCCGAAAGTG+AGG | 0.553005 | 7.1:+2468436 | None:intergenic |
| AAAATTGAAACCTGTAAGAG+AGG | 0.557194 | 7.1:+2468239 | None:intergenic |
| ATAGGCAAAGGAAGAGTTGA+CGG | 0.558591 | 7.1:+2467027 | None:intergenic |
| TAGTTAGATTCTTTGCTCAA+CGG | 0.559584 | 7.1:-2470419 | MS.gene066538:CDS |
| CTGATGCATGGTGTCTGTGT+GGG | 0.560006 | 7.1:-2472713 | MS.gene066538:CDS |
| GCCGAAACACTGAAGCCCGT+TGG | 0.562867 | 7.1:+2472520 | None:intergenic |
| TGTCCCAGGATCAGTAAAGC+TGG | 0.563242 | 7.1:+2466833 | None:intergenic |
| TTGAACGTGTACCAGGAGCC+AGG | 0.563333 | 7.1:+2470193 | None:intergenic |
| TACTTGCAGAATAATTGCAT+AGG | 0.565761 | 7.1:-2466466 | MS.gene066538:intron |
| TCGGGCAACCTAAACTTGCA+AGG | 0.567337 | 7.1:-2472670 | MS.gene066538:CDS |
| TACTAAGGCAAAGCTTTGCA+TGG | 0.571822 | 7.1:+2464185 | None:intergenic |
| AGGTTTGGATGACATAAGAA+AGG | 0.577567 | 7.1:-2470522 | MS.gene066538:intron |
| AAAACCTCACCTGTACAACA+TGG | 0.578089 | 7.1:+2464380 | None:intergenic |
| TTTGGATGGTCTGATGAAGG+AGG | 0.579418 | 7.1:-2471141 | MS.gene066538:CDS |
| CTCGGTGCATACCCCGGCCA+AGG | 0.582253 | 7.1:+2470571 | None:intergenic |
| TTGGATGGTCTGATGAAGGA+GGG | 0.582343 | 7.1:-2471140 | MS.gene066538:CDS |
| TTACTATGCAGGTTCCTCAA+AGG | 0.584607 | 7.1:-2472350 | MS.gene066538:intron |
| CCAAAGCTGTCACCAATCCA+TGG | 0.587050 | 7.1:+2468529 | None:intergenic |
| GGTTCTCTCAATATTCAAGC+TGG | 0.588540 | 7.1:-2471119 | MS.gene066538:CDS |
| CACAGGAGATAGAAGAGACA+TGG | 0.591418 | 7.1:+2472287 | None:intergenic |
| AAGATACTCGGTGCATACCC+CGG | 0.592613 | 7.1:+2470565 | None:intergenic |
| TGACCATCACTGTGGATGGA+TGG | 0.596777 | 7.1:-2466586 | MS.gene066538:intron |
| CGGGCAACCTAAACTTGCAA+GGG | 0.599823 | 7.1:-2472669 | MS.gene066538:CDS |
| TAGTAACACTACCACTAATG+AGG | 0.600625 | 7.1:-2464167 | MS.gene066538:intron |
| GAGCCGCTTCATGCCGACGC+AGG | 0.600999 | 7.1:+2470330 | None:intergenic |
| AGTGGCAAGACTACATGGAC+TGG | 0.601667 | 7.1:-2463468 | MS.gene066538:CDS |
| TGCAAGTTTAGGTTGCCCGA+GGG | 0.604363 | 7.1:+2472673 | None:intergenic |
| GATTTGACCATCACTGTGGA+TGG | 0.605648 | 7.1:-2466590 | MS.gene066538:CDS |
| TTGCACTTAAATAGTATCCA+GGG | 0.606733 | 7.1:+2467002 | None:intergenic |
| ACTCCTGCGTCGGCATGAAG+CGG | 0.614282 | 7.1:-2470333 | MS.gene066538:CDS |
| GAACCAAGAGTTGTATCCAC+AGG | 0.614813 | 7.1:+2472270 | None:intergenic |
| TAACCATCCATCCACAGTGA+TGG | 0.615375 | 7.1:+2466583 | None:intergenic |
| GCGCTAAAACAAAGTATCAG+TGG | 0.615716 | 7.1:-2463409 | MS.gene066538:CDS |
| ACACAGTCTTACTCCTGCGT+CGG | 0.616405 | 7.1:-2470343 | MS.gene066538:CDS |
| GTATCCAGGGATATAGGCAA+AGG | 0.616666 | 7.1:+2467015 | None:intergenic |
| CTGTATCGCAGACCTTCAAG+TGG | 0.619216 | 7.1:-2463486 | MS.gene066538:intron |
| GATTTACAAGGCAAACCCAA+CGG | 0.620582 | 7.1:-2472536 | MS.gene066538:CDS |
| CATGTCTCTTCTATCTCCTG+TGG | 0.624063 | 7.1:-2472286 | MS.gene066538:CDS |
| TTCATGTATAGCCTTGGCCG+GGG | 0.624932 | 7.1:-2470582 | MS.gene066538:CDS |
| TTGCAAGTTTAGGTTGCCCG+AGG | 0.626994 | 7.1:+2472672 | None:intergenic |
| AAACCTCACCTGTACAACAT+GGG | 0.629986 | 7.1:+2464381 | None:intergenic |
| TGACTTGAGTAACCCTGTTG+TGG | 0.633706 | 7.1:+2471318 | None:intergenic |
| TCTGATGCATGGTGTCTGTG+TGG | 0.635410 | 7.1:-2472714 | None:intergenic |
| AACCTCACCTGTACAACATG+GGG | 0.636619 | 7.1:+2464382 | None:intergenic |
| AAATCCATATATAAGATACT+CGG | 0.638034 | 7.1:+2470553 | None:intergenic |
| CTTGCAGAATAATTGCATAG+GGG | 0.645521 | 7.1:-2466464 | MS.gene066538:intron |
| ATTGCTTGAAAGTGTAGACA+AGG | 0.647018 | 7.1:-2472603 | MS.gene066538:CDS |
| GTGTCATGCAGGTTAACTCG+GGG | 0.654440 | 7.1:-2470751 | MS.gene066538:intron |
| ATTGTTGGGATGCTGTTAGG+CGG | 0.658661 | 7.1:-2464220 | MS.gene066538:CDS |
| GAGAAACTCAAAATCCACAA+AGG | 0.662893 | 7.1:+2471091 | None:intergenic |
| AAATTGGCCACCGACCGCAA+AGG | 0.671025 | 7.1:+2468493 | None:intergenic |
| CGGGCTTCAGTGTTTCGGCG+CGG | 0.680129 | 7.1:-2472516 | MS.gene066538:CDS |
| GAGAATCCAATGCTCAACCA+AGG | 0.684085 | 7.1:-2472645 | MS.gene066538:CDS |
| CAGTCAGAAATACCACAACA+GGG | 0.684532 | 7.1:-2471330 | MS.gene066538:CDS |
| ATTGGTTTGAGTCTGATGCA+TGG | 0.684860 | 7.1:-2472725 | None:intergenic |
| AGACAAGGAGCTAACAAAAG+GGG | 0.696044 | 7.1:-2472588 | MS.gene066538:CDS |
| ACAACTACCACATCCTCCAG+AGG | 0.699566 | 7.1:+2463325 | None:intergenic |
| GCACGATTTGACCATCACTG+TGG | 0.771092 | 7.1:-2466594 | MS.gene066538:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 2463122 | 2472732 | 2463122 | ID=MS.gene066538 |
| chr7.1 | mRNA | 2463122 | 2472732 | 2463122 | ID=MS.gene066538.t1;Parent=MS.gene066538 |
| chr7.1 | exon | 2472508 | 2472732 | 2472508 | ID=MS.gene066538.t1.exon1;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2472508 | 2472732 | 2472508 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2472143 | 2472361 | 2472143 | ID=MS.gene066538.t1.exon2;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2472143 | 2472361 | 2472143 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2471322 | 2471408 | 2471322 | ID=MS.gene066538.t1.exon3;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2471322 | 2471408 | 2471322 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2471085 | 2471231 | 2471085 | ID=MS.gene066538.t1.exon4;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2471085 | 2471231 | 2471085 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2470736 | 2470762 | 2470736 | ID=MS.gene066538.t1.exon5;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2470736 | 2470762 | 2470736 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2470523 | 2470618 | 2470523 | ID=MS.gene066538.t1.exon6;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2470523 | 2470618 | 2470523 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2470329 | 2470438 | 2470329 | ID=MS.gene066538.t1.exon7;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2470329 | 2470438 | 2470329 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2470205 | 2470245 | 2470205 | ID=MS.gene066538.t1.exon8;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2470205 | 2470245 | 2470205 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2470033 | 2470116 | 2470033 | ID=MS.gene066538.t1.exon9;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2470033 | 2470116 | 2470033 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2468429 | 2468567 | 2468429 | ID=MS.gene066538.t1.exon10;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2468429 | 2468567 | 2468429 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2468250 | 2468343 | 2468250 | ID=MS.gene066538.t1.exon11;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2468250 | 2468343 | 2468250 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2466995 | 2467089 | 2466995 | ID=MS.gene066538.t1.exon12;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2466995 | 2467089 | 2466995 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2466738 | 2466905 | 2466738 | ID=MS.gene066538.t1.exon13;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2466738 | 2466905 | 2466738 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2466587 | 2466659 | 2466587 | ID=MS.gene066538.t1.exon14;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2466587 | 2466659 | 2466587 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2466427 | 2466479 | 2466427 | ID=MS.gene066538.t1.exon15;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2466427 | 2466479 | 2466427 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2464537 | 2464628 | 2464537 | ID=MS.gene066538.t1.exon16;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2464537 | 2464628 | 2464537 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2464390 | 2464442 | 2464390 | ID=MS.gene066538.t1.exon17;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2464390 | 2464442 | 2464390 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2464168 | 2464293 | 2464168 | ID=MS.gene066538.t1.exon18;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2464168 | 2464293 | 2464168 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2463302 | 2463497 | 2463302 | ID=MS.gene066538.t1.exon19;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2463302 | 2463497 | 2463302 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| chr7.1 | exon | 2463122 | 2463180 | 2463122 | ID=MS.gene066538.t1.exon20;Parent=MS.gene066538.t1 |
| chr7.1 | CDS | 2463122 | 2463180 | 2463122 | ID=cds.MS.gene066538.t1;Parent=MS.gene066538.t1 |
| Gene Sequence |
| Protein sequence |
