Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067477.t1 | XP_013465603.1 | 95.6 | 320 | 7 | 4 | 1 | 317 | 1 | 316 | 1.50E-139 | 505.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067477.t1 | Q9SAH7 | 48.8 | 324 | 128 | 7 | 3 | 317 | 4 | 298 | 1.4e-64 | 248.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067477.t1 | A0A072VC75 | 95.6 | 320 | 7 | 4 | 1 | 317 | 1 | 316 | 1.1e-139 | 505.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene067477.t1 | TF | WRKY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049413 | MS.gene067477 | 0.806095 | 9.72E-50 | -1.69E-46 |
| MS.gene04998 | MS.gene067477 | 0.818882 | 1.55E-52 | -1.69E-46 |
| MS.gene050961 | MS.gene067477 | 0.823718 | 1.19E-53 | -1.69E-46 |
| MS.gene051226 | MS.gene067477 | 0.854819 | 9.55E-62 | -1.69E-46 |
| MS.gene051837 | MS.gene067477 | 0.851829 | 6.88E-61 | -1.69E-46 |
| MS.gene051854 | MS.gene067477 | 0.809363 | 1.96E-50 | -1.69E-46 |
| MS.gene051959 | MS.gene067477 | 0.836369 | 9.74E-57 | -1.69E-46 |
| MS.gene051960 | MS.gene067477 | 0.830461 | 2.90E-55 | -1.69E-46 |
| MS.gene052053 | MS.gene067477 | 0.804665 | 1.94E-49 | -1.69E-46 |
| MS.gene052091 | MS.gene067477 | 0.80781 | 4.21E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene067477 | 0.844604 | 6.82E-59 | -1.69E-46 |
| MS.gene054617 | MS.gene067477 | 0.817016 | 4.10E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene067477 | 0.823103 | 1.66E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene067477 | 0.814157 | 1.78E-51 | -1.69E-46 |
| MS.gene055549 | MS.gene067477 | 0.843609 | 1.26E-58 | -1.69E-46 |
| MS.gene055550 | MS.gene067477 | 0.844134 | 9.12E-59 | -1.69E-46 |
| MS.gene055551 | MS.gene067477 | 0.830512 | 2.82E-55 | -1.69E-46 |
| MS.gene05624 | MS.gene067477 | 0.808152 | 3.56E-50 | -1.69E-46 |
| MS.gene056365 | MS.gene067477 | 0.811748 | 6.00E-51 | -1.69E-46 |
| MS.gene057093 | MS.gene067477 | 0.858937 | 5.85E-63 | -1.69E-46 |
| MS.gene057167 | MS.gene067477 | 0.828092 | 1.09E-54 | -1.69E-46 |
| MS.gene057422 | MS.gene067477 | 0.819497 | 1.13E-52 | -1.69E-46 |
| MS.gene057622 | MS.gene067477 | 0.820806 | 5.65E-53 | -1.69E-46 |
| MS.gene06023 | MS.gene067477 | 0.838925 | 2.15E-57 | -1.69E-46 |
| MS.gene060446 | MS.gene067477 | 0.817423 | 3.32E-52 | -1.69E-46 |
| MS.gene06061 | MS.gene067477 | 0.818187 | 2.23E-52 | -1.69E-46 |
| MS.gene060964 | MS.gene067477 | 0.858025 | 1.09E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067477.t1 | MTR_2g105060 | 95.639 | 321 | 5 | 5 | 1 | 317 | 1 | 316 | 0.0 | 614 |
| MS.gene067477.t1 | MTR_2g105060 | 96.748 | 246 | 1 | 3 | 74 | 317 | 1 | 241 | 8.59e-175 | 483 |
| MS.gene067477.t1 | MTR_4g007060 | 59.202 | 326 | 108 | 8 | 5 | 317 | 4 | 317 | 2.77e-105 | 310 |
| MS.gene067477.t1 | MTR_4g007060 | 57.491 | 287 | 102 | 6 | 44 | 317 | 4 | 283 | 6.31e-88 | 265 |
| MS.gene067477.t1 | MTR_1g013760 | 46.643 | 283 | 127 | 7 | 41 | 314 | 9 | 276 | 2.17e-71 | 223 |
| MS.gene067477.t1 | MTR_3g106060 | 46.182 | 275 | 115 | 7 | 56 | 314 | 29 | 286 | 8.74e-69 | 216 |
| MS.gene067477.t1 | MTR_4g007060 | 60.360 | 222 | 79 | 5 | 1 | 222 | 1 | 213 | 3.18e-68 | 213 |
| MS.gene067477.t1 | MTR_1g013790 | 36.567 | 268 | 134 | 7 | 51 | 316 | 6 | 239 | 1.39e-39 | 140 |
| MS.gene067477.t1 | MTR_7g110720 | 27.586 | 319 | 146 | 8 | 57 | 310 | 100 | 398 | 7.65e-30 | 118 |
| MS.gene067477.t1 | MTR_7g110720 | 28.247 | 308 | 131 | 9 | 57 | 310 | 100 | 371 | 1.07e-28 | 114 |
| MS.gene067477.t1 | MTR_4g132430 | 30.357 | 224 | 113 | 5 | 49 | 236 | 110 | 326 | 1.28e-27 | 112 |
| MS.gene067477.t1 | MTR_2g023930 | 32.911 | 237 | 95 | 8 | 49 | 231 | 139 | 365 | 4.62e-26 | 108 |
| MS.gene067477.t1 | MTR_3g053330 | 30.870 | 230 | 110 | 6 | 47 | 231 | 86 | 311 | 4.59e-24 | 102 |
| MS.gene067477.t1 | MTR_2g045360 | 31.579 | 266 | 123 | 8 | 55 | 275 | 70 | 321 | 1.47e-23 | 100 |
| MS.gene067477.t1 | MTR_4g094412 | 53.086 | 81 | 33 | 2 | 151 | 231 | 219 | 294 | 1.47e-23 | 100 |
| MS.gene067477.t1 | MTR_4g094412 | 53.086 | 81 | 33 | 2 | 151 | 231 | 228 | 303 | 1.51e-23 | 100 |
| MS.gene067477.t1 | MTR_4g107970 | 51.250 | 80 | 37 | 1 | 152 | 231 | 161 | 238 | 1.18e-22 | 98.6 |
| MS.gene067477.t1 | MTR_7g009730 | 47.619 | 84 | 42 | 1 | 146 | 229 | 196 | 277 | 1.58e-22 | 98.2 |
| MS.gene067477.t1 | MTR_4g107970 | 51.250 | 80 | 37 | 1 | 152 | 231 | 238 | 315 | 1.78e-22 | 98.2 |
| MS.gene067477.t1 | MTR_7g109800 | 50.617 | 81 | 37 | 2 | 151 | 231 | 127 | 204 | 3.77e-22 | 94.7 |
| MS.gene067477.t1 | MTR_1g086790 | 54.545 | 77 | 32 | 2 | 156 | 232 | 145 | 218 | 4.25e-22 | 95.1 |
| MS.gene067477.t1 | MTR_3g104750 | 40.000 | 120 | 60 | 4 | 112 | 229 | 52 | 161 | 4.30e-22 | 91.3 |
| MS.gene067477.t1 | MTR_4g132430 | 47.191 | 89 | 42 | 2 | 148 | 236 | 109 | 192 | 8.96e-22 | 94.7 |
| MS.gene067477.t1 | MTR_2g033820 | 53.086 | 81 | 35 | 2 | 156 | 236 | 190 | 267 | 1.21e-21 | 94.4 |
| MS.gene067477.t1 | MTR_4g094588 | 51.948 | 77 | 34 | 2 | 156 | 232 | 165 | 238 | 2.17e-21 | 93.2 |
| MS.gene067477.t1 | MTR_4g049760 | 50.000 | 78 | 37 | 1 | 155 | 232 | 276 | 351 | 3.55e-21 | 94.4 |
| MS.gene067477.t1 | MTR_7g079010 | 52.564 | 78 | 34 | 2 | 152 | 229 | 124 | 198 | 7.35e-21 | 89.4 |
| MS.gene067477.t1 | MTR_2g088000 | 31.048 | 248 | 89 | 9 | 56 | 231 | 172 | 409 | 2.04e-20 | 92.0 |
| MS.gene067477.t1 | MTR_4g007060 | 50.794 | 126 | 59 | 2 | 44 | 169 | 4 | 126 | 4.02e-20 | 86.3 |
| MS.gene067477.t1 | MTR_5g094430 | 57.812 | 64 | 26 | 1 | 169 | 232 | 228 | 290 | 4.52e-20 | 90.1 |
| MS.gene067477.t1 | MTR_5g094430 | 46.269 | 67 | 31 | 3 | 172 | 238 | 67 | 128 | 1.39e-11 | 65.1 |
| MS.gene067477.t1 | MTR_5g094430 | 57.812 | 64 | 26 | 1 | 169 | 232 | 250 | 312 | 5.22e-20 | 90.1 |
| MS.gene067477.t1 | MTR_5g094430 | 46.269 | 67 | 31 | 3 | 172 | 238 | 89 | 150 | 1.39e-11 | 65.1 |
| MS.gene067477.t1 | MTR_4g049790 | 35.000 | 140 | 58 | 3 | 155 | 263 | 298 | 435 | 5.71e-20 | 90.9 |
| MS.gene067477.t1 | MTR_5g094430 | 57.812 | 64 | 26 | 1 | 169 | 232 | 362 | 424 | 5.75e-20 | 90.5 |
| MS.gene067477.t1 | MTR_5g094430 | 46.269 | 67 | 31 | 3 | 172 | 238 | 201 | 262 | 1.54e-11 | 65.5 |
| MS.gene067477.t1 | MTR_4g013260 | 40.909 | 110 | 49 | 2 | 122 | 231 | 302 | 395 | 7.04e-20 | 90.1 |
| MS.gene067477.t1 | MTR_4g013260 | 49.231 | 65 | 32 | 1 | 171 | 235 | 184 | 247 | 4.49e-16 | 79.0 |
| MS.gene067477.t1 | MTR_7g100510 | 52.857 | 70 | 32 | 1 | 162 | 231 | 352 | 420 | 9.15e-20 | 89.7 |
| MS.gene067477.t1 | MTR_7g100510 | 53.333 | 60 | 26 | 2 | 172 | 231 | 190 | 247 | 1.25e-14 | 74.3 |
| MS.gene067477.t1 | MTR_3g056100 | 57.812 | 64 | 26 | 1 | 169 | 232 | 256 | 318 | 9.23e-20 | 89.7 |
| MS.gene067477.t1 | MTR_3g056100 | 48.333 | 60 | 29 | 2 | 172 | 231 | 100 | 157 | 1.79e-11 | 65.1 |
| MS.gene067477.t1 | MTR_3g056100 | 57.812 | 64 | 26 | 1 | 169 | 232 | 269 | 331 | 1.01e-19 | 89.7 |
| MS.gene067477.t1 | MTR_3g056100 | 48.333 | 60 | 29 | 2 | 172 | 231 | 100 | 157 | 1.83e-11 | 65.1 |
| MS.gene067477.t1 | MTR_5g042920 | 54.688 | 64 | 28 | 1 | 169 | 232 | 318 | 380 | 1.07e-19 | 89.4 |
| MS.gene067477.t1 | MTR_5g042920 | 45.455 | 77 | 32 | 4 | 172 | 246 | 152 | 220 | 7.65e-13 | 68.9 |
| MS.gene067477.t1 | MTR_3g056100 | 57.812 | 64 | 26 | 1 | 169 | 232 | 413 | 475 | 1.14e-19 | 89.7 |
| MS.gene067477.t1 | MTR_3g056100 | 48.333 | 60 | 29 | 2 | 172 | 231 | 244 | 301 | 1.89e-11 | 65.1 |
| MS.gene067477.t1 | MTR_3g056100 | 57.812 | 64 | 26 | 1 | 169 | 232 | 400 | 462 | 1.16e-19 | 89.7 |
| MS.gene067477.t1 | MTR_3g056100 | 48.333 | 60 | 29 | 2 | 172 | 231 | 244 | 301 | 1.97e-11 | 65.1 |
| MS.gene067477.t1 | MTR_4g082580 | 42.056 | 107 | 46 | 3 | 137 | 243 | 259 | 349 | 1.23e-19 | 89.0 |
| MS.gene067477.t1 | MTR_4g082580 | 48.529 | 68 | 34 | 1 | 172 | 239 | 146 | 212 | 4.43e-13 | 69.7 |
| MS.gene067477.t1 | MTR_5g091390 | 50.649 | 77 | 35 | 2 | 156 | 232 | 144 | 217 | 1.47e-19 | 87.8 |
| MS.gene067477.t1 | MTR_7g080000 | 42.478 | 113 | 61 | 3 | 121 | 232 | 383 | 492 | 3.32e-19 | 88.6 |
| MS.gene067477.t1 | MTR_7g080000 | 46.774 | 62 | 31 | 2 | 170 | 231 | 233 | 292 | 9.57e-11 | 63.2 |
| MS.gene067477.t1 | MTR_1g015140 | 50.667 | 75 | 34 | 2 | 156 | 230 | 89 | 160 | 4.19e-19 | 83.2 |
| MS.gene067477.t1 | MTR_3g009470 | 41.667 | 96 | 40 | 2 | 137 | 232 | 476 | 555 | 6.37e-19 | 87.8 |
| MS.gene067477.t1 | MTR_3g009470 | 49.180 | 61 | 29 | 2 | 171 | 231 | 280 | 338 | 2.90e-12 | 67.8 |
| MS.gene067477.t1 | MTR_7g011820 | 56.667 | 60 | 25 | 1 | 169 | 228 | 408 | 466 | 7.76e-19 | 87.4 |
| MS.gene067477.t1 | MTR_7g011820 | 43.373 | 83 | 43 | 3 | 172 | 252 | 229 | 309 | 6.55e-12 | 66.6 |
| MS.gene067477.t1 | MTR_5g074400 | 40.741 | 108 | 48 | 3 | 125 | 232 | 389 | 480 | 8.22e-19 | 87.4 |
| MS.gene067477.t1 | MTR_5g074400 | 50.794 | 63 | 29 | 2 | 172 | 234 | 244 | 304 | 5.02e-14 | 72.8 |
| MS.gene067477.t1 | MTR_7g071120 | 35.075 | 134 | 64 | 4 | 126 | 252 | 294 | 411 | 8.98e-19 | 87.0 |
| MS.gene067477.t1 | MTR_7g071120 | 45.714 | 70 | 36 | 2 | 171 | 240 | 166 | 233 | 2.75e-14 | 73.6 |
| MS.gene067477.t1 | MTR_5g016610 | 40.385 | 104 | 55 | 2 | 125 | 228 | 160 | 256 | 1.06e-18 | 84.7 |
| MS.gene067477.t1 | MTR_8g092140 | 49.315 | 73 | 35 | 2 | 156 | 228 | 146 | 216 | 1.21e-18 | 83.6 |
| MS.gene067477.t1 | MTR_5g043880 | 50.685 | 73 | 33 | 2 | 156 | 228 | 111 | 180 | 1.31e-18 | 84.3 |
| MS.gene067477.t1 | MTR_7g080020 | 44.545 | 110 | 54 | 4 | 126 | 232 | 448 | 553 | 1.74e-18 | 86.7 |
| MS.gene067477.t1 | MTR_7g080020 | 49.231 | 65 | 29 | 3 | 171 | 233 | 280 | 342 | 1.28e-11 | 65.9 |
| MS.gene067477.t1 | MTR_7g028415 | 38.095 | 126 | 64 | 3 | 115 | 229 | 28 | 150 | 1.89e-18 | 81.6 |
| MS.gene067477.t1 | MTR_7g028710 | 38.095 | 126 | 64 | 3 | 115 | 229 | 28 | 150 | 1.89e-18 | 81.6 |
| MS.gene067477.t1 | MTR_8g096730 | 44.872 | 78 | 42 | 1 | 169 | 246 | 68 | 144 | 3.12e-18 | 80.5 |
| MS.gene067477.t1 | MTR_1g009613 | 44.048 | 84 | 43 | 1 | 149 | 228 | 236 | 319 | 5.22e-18 | 83.6 |
| MS.gene067477.t1 | MTR_1g077780 | 50.000 | 64 | 31 | 1 | 169 | 232 | 60 | 122 | 5.48e-18 | 79.7 |
| MS.gene067477.t1 | MTR_4g122530 | 49.351 | 77 | 36 | 2 | 155 | 231 | 194 | 267 | 5.53e-18 | 84.3 |
| MS.gene067477.t1 | MTR_3g095040 | 30.570 | 193 | 110 | 6 | 88 | 273 | 30 | 205 | 5.70e-18 | 81.3 |
| MS.gene067477.t1 | MTR_3g031220 | 39.091 | 110 | 51 | 3 | 123 | 232 | 332 | 425 | 6.30e-18 | 84.3 |
| MS.gene067477.t1 | MTR_3g031220 | 50.000 | 60 | 28 | 2 | 172 | 231 | 199 | 256 | 6.12e-14 | 72.4 |
| MS.gene067477.t1 | MTR_4g094908 | 56.140 | 57 | 25 | 0 | 172 | 228 | 267 | 323 | 1.30e-17 | 82.4 |
| MS.gene067477.t1 | MTR_5g097490 | 50.000 | 78 | 36 | 2 | 155 | 232 | 238 | 312 | 1.42e-17 | 82.8 |
| MS.gene067477.t1 | MTR_3g095080 | 47.945 | 73 | 35 | 2 | 156 | 228 | 131 | 200 | 2.58e-17 | 79.7 |
| MS.gene067477.t1 | MTR_3g095080 | 47.945 | 73 | 35 | 2 | 156 | 228 | 155 | 224 | 2.68e-17 | 80.1 |
| MS.gene067477.t1 | MTR_8g092010 | 47.368 | 76 | 37 | 2 | 156 | 231 | 95 | 167 | 8.23e-17 | 77.4 |
| MS.gene067477.t1 | MTR_8g007270 | 39.130 | 115 | 60 | 4 | 115 | 228 | 105 | 210 | 9.78e-17 | 78.2 |
| MS.gene067477.t1 | MTR_2g032030 | 42.857 | 77 | 44 | 0 | 168 | 244 | 264 | 340 | 1.18e-16 | 80.5 |
| MS.gene067477.t1 | MTR_2g032030 | 43.038 | 79 | 45 | 0 | 168 | 246 | 264 | 342 | 1.21e-16 | 80.5 |
| MS.gene067477.t1 | MTR_1g070455 | 51.667 | 60 | 29 | 0 | 169 | 228 | 291 | 350 | 1.56e-16 | 79.7 |
| MS.gene067477.t1 | MTR_7g109600 | 35.238 | 105 | 65 | 1 | 135 | 239 | 20 | 121 | 1.84e-16 | 78.2 |
| MS.gene067477.t1 | MTR_7g062220 | 45.333 | 75 | 30 | 2 | 172 | 236 | 125 | 198 | 5.57e-16 | 77.4 |
| MS.gene067477.t1 | MTR_4g127040 | 45.570 | 79 | 42 | 1 | 151 | 228 | 224 | 302 | 7.01e-16 | 77.4 |
| MS.gene067477.t1 | MTR_8g087000 | 34.821 | 112 | 72 | 1 | 140 | 250 | 45 | 156 | 9.42e-16 | 75.9 |
| MS.gene067477.t1 | MTR_8g098945 | 46.774 | 62 | 33 | 0 | 172 | 233 | 130 | 191 | 1.44e-15 | 75.9 |
| MS.gene067477.t1 | MTR_8g028565 | 37.895 | 95 | 53 | 2 | 172 | 266 | 156 | 244 | 1.76e-15 | 76.3 |
| MS.gene067477.t1 | MTR_8g087000 | 42.500 | 80 | 45 | 1 | 172 | 250 | 78 | 157 | 2.16e-15 | 74.7 |
| MS.gene067477.t1 | MTR_3g108960 | 31.818 | 110 | 58 | 2 | 124 | 228 | 274 | 371 | 7.23e-15 | 75.1 |
| MS.gene067477.t1 | MTR_4g130900 | 43.836 | 73 | 40 | 1 | 159 | 231 | 347 | 418 | 8.87e-15 | 75.1 |
| MS.gene067477.t1 | MTR_7g079040 | 43.077 | 65 | 37 | 0 | 169 | 233 | 152 | 216 | 1.22e-14 | 73.9 |
| MS.gene067477.t1 | MTR_0117s0040 | 43.939 | 66 | 37 | 0 | 163 | 228 | 66 | 131 | 1.37e-14 | 72.4 |
| MS.gene067477.t1 | MTR_4g047580 | 38.462 | 78 | 46 | 1 | 155 | 232 | 267 | 342 | 2.08e-14 | 73.9 |
| MS.gene067477.t1 | MTR_3g031220 | 50.000 | 60 | 28 | 2 | 172 | 231 | 199 | 256 | 3.37e-14 | 72.8 |
| MS.gene067477.t1 | MTR_2g016770 | 45.000 | 60 | 33 | 0 | 172 | 231 | 36 | 95 | 5.00e-14 | 70.5 |
| MS.gene067477.t1 | MTR_2g016770 | 45.000 | 60 | 33 | 0 | 172 | 231 | 28 | 87 | 6.62e-14 | 70.1 |
| MS.gene067477.t1 | MTR_2g024350 | 45.161 | 62 | 34 | 0 | 171 | 232 | 8 | 69 | 8.05e-14 | 70.1 |
| MS.gene067477.t1 | MTR_1g078260 | 44.595 | 74 | 37 | 3 | 156 | 228 | 234 | 304 | 1.19e-13 | 71.6 |
| MS.gene067477.t1 | MTR_2g083870 | 36.364 | 110 | 66 | 2 | 125 | 231 | 32 | 140 | 1.65e-13 | 69.7 |
| MS.gene067477.t1 | MTR_2g024380 | 46.774 | 62 | 33 | 0 | 171 | 232 | 251 | 312 | 1.83e-13 | 71.2 |
| MS.gene067477.t1 | MTR_6g086555 | 39.506 | 81 | 45 | 3 | 156 | 235 | 193 | 270 | 2.07e-13 | 70.9 |
| MS.gene067477.t1 | MTR_5g074200 | 43.284 | 67 | 36 | 2 | 172 | 238 | 221 | 285 | 5.83e-13 | 68.9 |
| MS.gene067477.t1 | MTR_1g099600 | 43.548 | 62 | 35 | 0 | 172 | 233 | 186 | 247 | 7.16e-13 | 68.6 |
| MS.gene067477.t1 | MTR_3g009470 | 34.783 | 115 | 47 | 4 | 171 | 285 | 280 | 366 | 2.57e-12 | 67.8 |
| MS.gene067477.t1 | MTR_5g097500 | 38.667 | 75 | 45 | 1 | 158 | 232 | 217 | 290 | 2.73e-12 | 66.6 |
| MS.gene067477.t1 | MTR_2g075610 | 42.029 | 69 | 38 | 1 | 161 | 227 | 19 | 87 | 3.82e-12 | 64.7 |
| MS.gene067477.t1 | MTR_8g067650 | 44.444 | 72 | 35 | 1 | 169 | 240 | 337 | 403 | 8.76e-12 | 66.2 |
| MS.gene067477.t1 | MTR_8g099350 | 47.059 | 68 | 32 | 2 | 163 | 226 | 122 | 189 | 9.32e-12 | 65.5 |
| MS.gene067477.t1 | MTR_8g067635 | 44.444 | 72 | 35 | 1 | 169 | 240 | 347 | 413 | 9.69e-12 | 65.9 |
| MS.gene067477.t1 | MTR_2g075750 | 41.176 | 68 | 38 | 1 | 161 | 226 | 18 | 85 | 1.11e-11 | 63.2 |
| MS.gene067477.t1 | MTR_5g067680 | 32.479 | 117 | 69 | 4 | 120 | 226 | 143 | 259 | 4.39e-11 | 63.5 |
| MS.gene067477.t1 | MTR_8g069935 | 42.623 | 61 | 35 | 0 | 171 | 231 | 331 | 391 | 6.05e-11 | 63.5 |
| MS.gene067477.t1 | MTR_6g053200 | 42.188 | 64 | 37 | 0 | 169 | 232 | 328 | 391 | 9.14e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067477.t1 | AT1G80840 | 53.776 | 331 | 106 | 12 | 1 | 317 | 1 | 298 | 2.15e-99 | 295 |
| MS.gene067477.t1 | AT4G31800 | 45.238 | 336 | 135 | 10 | 1 | 316 | 1 | 307 | 1.58e-79 | 244 |
| MS.gene067477.t1 | AT4G31800 | 47.328 | 262 | 99 | 7 | 74 | 316 | 1 | 242 | 2.56e-68 | 213 |
| MS.gene067477.t1 | AT2G25000 | 45.283 | 265 | 95 | 7 | 57 | 314 | 2 | 223 | 1.64e-63 | 201 |
| MS.gene067477.t1 | AT2G25000 | 45.283 | 265 | 95 | 7 | 57 | 314 | 2 | 223 | 1.64e-63 | 201 |
| MS.gene067477.t1 | AT2G25000 | 44.364 | 275 | 103 | 7 | 49 | 316 | 37 | 268 | 2.08e-63 | 202 |
| MS.gene067477.t1 | AT2G25000 | 43.599 | 289 | 110 | 8 | 33 | 314 | 3 | 245 | 5.32e-63 | 200 |
| MS.gene067477.t1 | AT4G31800 | 46.053 | 228 | 88 | 8 | 1 | 208 | 1 | 213 | 1.42e-49 | 165 |
| MS.gene067477.t1 | AT5G41570 | 48.936 | 94 | 44 | 3 | 136 | 229 | 66 | 155 | 2.65e-23 | 95.1 |
| MS.gene067477.t1 | AT4G18170 | 47.525 | 101 | 49 | 3 | 133 | 232 | 135 | 232 | 6.59e-23 | 97.1 |
| MS.gene067477.t1 | AT4G01720 | 24.857 | 350 | 153 | 8 | 60 | 310 | 105 | 443 | 6.82e-23 | 99.0 |
| MS.gene067477.t1 | AT1G64000 | 52.632 | 76 | 33 | 2 | 154 | 229 | 99 | 171 | 8.26e-23 | 94.0 |
| MS.gene067477.t1 | AT4G04450 | 39.252 | 107 | 56 | 1 | 125 | 231 | 161 | 258 | 8.95e-23 | 98.2 |
| MS.gene067477.t1 | AT1G68150 | 51.852 | 81 | 34 | 2 | 151 | 231 | 220 | 295 | 1.20e-22 | 97.4 |
| MS.gene067477.t1 | AT4G22070 | 50.000 | 84 | 37 | 2 | 148 | 231 | 279 | 357 | 1.69e-22 | 98.2 |
| MS.gene067477.t1 | AT4G22070 | 50.000 | 84 | 37 | 2 | 148 | 231 | 279 | 357 | 1.69e-22 | 98.2 |
| MS.gene067477.t1 | AT4G22070 | 50.000 | 84 | 37 | 2 | 148 | 231 | 279 | 357 | 1.69e-22 | 98.2 |
| MS.gene067477.t1 | AT4G22070 | 50.000 | 84 | 37 | 2 | 148 | 231 | 279 | 357 | 1.69e-22 | 98.2 |
| MS.gene067477.t1 | AT4G04450 | 39.252 | 107 | 56 | 1 | 125 | 231 | 255 | 352 | 2.71e-22 | 97.4 |
| MS.gene067477.t1 | AT1G29860 | 52.439 | 82 | 36 | 2 | 156 | 237 | 123 | 201 | 2.77e-22 | 94.7 |
| MS.gene067477.t1 | AT1G62300 | 50.000 | 84 | 37 | 2 | 148 | 231 | 294 | 372 | 4.18e-22 | 97.1 |
| MS.gene067477.t1 | AT4G26640 | 31.527 | 203 | 121 | 5 | 113 | 310 | 144 | 333 | 7.52e-22 | 95.1 |
| MS.gene067477.t1 | AT4G26640 | 43.662 | 71 | 38 | 2 | 161 | 231 | 25 | 93 | 3.33e-12 | 67.0 |
| MS.gene067477.t1 | AT4G26640 | 31.527 | 203 | 121 | 5 | 113 | 310 | 144 | 333 | 7.52e-22 | 95.1 |
| MS.gene067477.t1 | AT4G26640 | 43.662 | 71 | 38 | 2 | 161 | 231 | 25 | 93 | 3.33e-12 | 67.0 |
| MS.gene067477.t1 | AT4G26640 | 31.527 | 203 | 121 | 5 | 113 | 310 | 144 | 333 | 7.52e-22 | 95.1 |
| MS.gene067477.t1 | AT4G26640 | 43.662 | 71 | 38 | 2 | 161 | 231 | 25 | 93 | 3.33e-12 | 67.0 |
| MS.gene067477.t1 | AT4G26640 | 31.527 | 203 | 121 | 5 | 113 | 310 | 144 | 333 | 7.52e-22 | 95.1 |
| MS.gene067477.t1 | AT4G26640 | 43.662 | 71 | 38 | 2 | 161 | 231 | 25 | 93 | 3.33e-12 | 67.0 |
| MS.gene067477.t1 | AT5G15130 | 38.793 | 116 | 55 | 3 | 121 | 231 | 183 | 287 | 1.17e-21 | 95.9 |
| MS.gene067477.t1 | AT5G15130 | 38.793 | 116 | 55 | 3 | 121 | 231 | 141 | 245 | 1.18e-21 | 95.5 |
| MS.gene067477.t1 | AT1G18860 | 58.065 | 62 | 26 | 0 | 170 | 231 | 238 | 299 | 1.73e-21 | 95.1 |
| MS.gene067477.t1 | AT4G26640 | 31.527 | 203 | 121 | 5 | 113 | 310 | 248 | 437 | 1.79e-21 | 94.7 |
| MS.gene067477.t1 | AT4G26640 | 43.662 | 71 | 38 | 2 | 161 | 231 | 129 | 197 | 3.55e-12 | 67.4 |
| MS.gene067477.t1 | AT4G26640 | 31.527 | 203 | 121 | 5 | 113 | 310 | 280 | 469 | 2.57e-21 | 94.7 |
| MS.gene067477.t1 | AT4G26640 | 43.662 | 71 | 38 | 2 | 161 | 231 | 161 | 229 | 3.74e-12 | 67.4 |
| MS.gene067477.t1 | AT4G26640 | 31.527 | 203 | 121 | 5 | 113 | 310 | 320 | 509 | 2.80e-21 | 94.7 |
| MS.gene067477.t1 | AT4G26640 | 43.662 | 71 | 38 | 2 | 161 | 231 | 201 | 269 | 3.81e-12 | 67.4 |
| MS.gene067477.t1 | AT5G07100 | 38.983 | 118 | 56 | 3 | 115 | 232 | 100 | 201 | 2.98e-21 | 90.5 |
| MS.gene067477.t1 | AT5G07100 | 45.455 | 77 | 41 | 1 | 159 | 235 | 12 | 87 | 3.40e-16 | 76.6 |
| MS.gene067477.t1 | AT5G07100 | 38.983 | 118 | 56 | 3 | 115 | 232 | 100 | 201 | 2.98e-21 | 90.5 |
| MS.gene067477.t1 | AT5G07100 | 45.455 | 77 | 41 | 1 | 159 | 235 | 12 | 87 | 3.40e-16 | 76.6 |
| MS.gene067477.t1 | AT5G07100 | 38.983 | 118 | 56 | 3 | 115 | 232 | 100 | 201 | 2.98e-21 | 90.5 |
| MS.gene067477.t1 | AT5G07100 | 45.455 | 77 | 41 | 1 | 159 | 235 | 12 | 87 | 3.40e-16 | 76.6 |
| MS.gene067477.t1 | AT5G07100 | 38.983 | 118 | 56 | 3 | 115 | 232 | 100 | 201 | 2.98e-21 | 90.5 |
| MS.gene067477.t1 | AT5G07100 | 45.455 | 77 | 41 | 1 | 159 | 235 | 12 | 87 | 3.40e-16 | 76.6 |
| MS.gene067477.t1 | AT2G47260 | 50.000 | 86 | 40 | 2 | 151 | 236 | 156 | 238 | 4.95e-21 | 92.4 |
| MS.gene067477.t1 | AT5G49520 | 51.948 | 77 | 34 | 2 | 155 | 231 | 207 | 280 | 5.80e-21 | 92.8 |
| MS.gene067477.t1 | AT5G07100 | 33.987 | 153 | 80 | 4 | 85 | 232 | 158 | 294 | 6.83e-21 | 91.3 |
| MS.gene067477.t1 | AT5G07100 | 45.455 | 77 | 41 | 1 | 159 | 235 | 105 | 180 | 1.12e-15 | 76.6 |
| MS.gene067477.t1 | AT3G62340 | 51.163 | 86 | 39 | 2 | 151 | 236 | 100 | 182 | 9.29e-21 | 90.5 |
| MS.gene067477.t1 | AT2G21900 | 45.000 | 100 | 47 | 3 | 134 | 232 | 77 | 169 | 1.28e-20 | 88.6 |
| MS.gene067477.t1 | AT5G26170 | 53.425 | 73 | 31 | 2 | 156 | 228 | 100 | 169 | 1.87e-20 | 87.0 |
| MS.gene067477.t1 | AT1G13960 | 34.694 | 147 | 68 | 3 | 101 | 247 | 366 | 484 | 2.09e-20 | 92.0 |
| MS.gene067477.t1 | AT1G13960 | 50.746 | 67 | 31 | 2 | 172 | 238 | 230 | 294 | 2.25e-13 | 70.9 |
| MS.gene067477.t1 | AT1G13960 | 34.694 | 147 | 68 | 3 | 101 | 247 | 213 | 331 | 3.93e-20 | 90.1 |
| MS.gene067477.t1 | AT1G13960 | 50.746 | 67 | 31 | 2 | 172 | 238 | 77 | 141 | 2.60e-13 | 70.1 |
| MS.gene067477.t1 | AT5G46350 | 40.476 | 126 | 54 | 6 | 156 | 280 | 170 | 275 | 4.20e-20 | 89.4 |
| MS.gene067477.t1 | AT1G69310 | 50.000 | 78 | 36 | 2 | 151 | 228 | 129 | 203 | 4.92e-20 | 88.6 |
| MS.gene067477.t1 | AT1G69310 | 50.000 | 78 | 36 | 2 | 151 | 228 | 129 | 203 | 4.92e-20 | 88.6 |
| MS.gene067477.t1 | AT1G69310 | 50.000 | 78 | 36 | 2 | 151 | 228 | 129 | 203 | 4.92e-20 | 88.6 |
| MS.gene067477.t1 | AT5G56270 | 44.000 | 100 | 40 | 3 | 133 | 232 | 464 | 547 | 7.54e-20 | 90.5 |
| MS.gene067477.t1 | AT5G56270 | 44.737 | 76 | 36 | 3 | 170 | 245 | 272 | 341 | 1.53e-12 | 68.6 |
| MS.gene067477.t1 | AT2G03340 | 56.250 | 64 | 27 | 1 | 169 | 232 | 413 | 475 | 9.07e-20 | 90.1 |
| MS.gene067477.t1 | AT2G03340 | 47.222 | 72 | 36 | 2 | 172 | 243 | 251 | 320 | 4.27e-14 | 73.2 |
| MS.gene067477.t1 | AT5G13080 | 50.000 | 80 | 37 | 2 | 156 | 235 | 54 | 130 | 1.65e-19 | 84.0 |
| MS.gene067477.t1 | AT1G55600 | 50.000 | 80 | 37 | 2 | 152 | 231 | 290 | 366 | 2.15e-19 | 89.0 |
| MS.gene067477.t1 | AT1G69810 | 29.365 | 252 | 113 | 8 | 43 | 231 | 15 | 264 | 1.08e-18 | 86.3 |
| MS.gene067477.t1 | AT3G01080 | 55.556 | 63 | 27 | 1 | 169 | 231 | 342 | 403 | 1.12e-18 | 86.7 |
| MS.gene067477.t1 | AT3G01080 | 56.667 | 60 | 25 | 1 | 169 | 228 | 304 | 362 | 1.28e-18 | 86.3 |
| MS.gene067477.t1 | AT2G30250 | 48.276 | 87 | 42 | 2 | 146 | 232 | 305 | 388 | 3.15e-18 | 84.7 |
| MS.gene067477.t1 | AT2G30250 | 35.577 | 104 | 52 | 4 | 172 | 262 | 167 | 268 | 1.14e-12 | 68.6 |
| MS.gene067477.t1 | AT5G52830 | 42.045 | 88 | 51 | 0 | 172 | 259 | 166 | 253 | 3.88e-18 | 84.3 |
| MS.gene067477.t1 | AT5G64810 | 37.931 | 116 | 67 | 3 | 113 | 228 | 56 | 166 | 4.35e-18 | 81.3 |
| MS.gene067477.t1 | AT3G01970 | 51.351 | 74 | 33 | 2 | 155 | 228 | 51 | 121 | 6.01e-18 | 79.7 |
| MS.gene067477.t1 | AT1G69810 | 48.750 | 80 | 38 | 2 | 153 | 231 | 129 | 206 | 8.36e-18 | 83.2 |
| MS.gene067477.t1 | AT2G23320 | 39.683 | 126 | 62 | 4 | 103 | 228 | 186 | 297 | 1.13e-17 | 82.4 |
| MS.gene067477.t1 | AT4G39410 | 45.783 | 83 | 42 | 2 | 146 | 228 | 200 | 279 | 1.41e-17 | 82.0 |
| MS.gene067477.t1 | AT2G46130 | 48.101 | 79 | 38 | 2 | 151 | 229 | 12 | 87 | 1.57e-17 | 77.4 |
| MS.gene067477.t1 | AT2G04880 | 50.704 | 71 | 34 | 1 | 169 | 239 | 305 | 374 | 1.82e-17 | 83.2 |
| MS.gene067477.t1 | AT2G04880 | 41.026 | 78 | 43 | 3 | 169 | 245 | 109 | 184 | 1.51e-12 | 68.6 |
| MS.gene067477.t1 | AT2G04880 | 50.704 | 71 | 34 | 1 | 169 | 239 | 281 | 350 | 2.02e-17 | 82.8 |
| MS.gene067477.t1 | AT2G04880 | 41.026 | 78 | 43 | 3 | 169 | 245 | 109 | 184 | 1.52e-12 | 68.2 |
| MS.gene067477.t1 | AT2G38470 | 53.125 | 64 | 29 | 1 | 169 | 232 | 360 | 422 | 2.77e-17 | 82.8 |
| MS.gene067477.t1 | AT2G38470 | 50.820 | 61 | 28 | 2 | 171 | 231 | 184 | 242 | 3.30e-14 | 73.6 |
| MS.gene067477.t1 | AT4G26440 | 54.688 | 64 | 28 | 1 | 169 | 232 | 370 | 432 | 3.79e-17 | 82.4 |
| MS.gene067477.t1 | AT4G26440 | 34.259 | 108 | 60 | 4 | 172 | 279 | 179 | 275 | 1.48e-11 | 65.5 |
| MS.gene067477.t1 | AT2G30590 | 51.515 | 66 | 31 | 1 | 169 | 233 | 311 | 376 | 4.48e-17 | 81.3 |
| MS.gene067477.t1 | AT4G31550 | 51.667 | 60 | 29 | 0 | 169 | 228 | 243 | 302 | 4.88e-17 | 80.9 |
| MS.gene067477.t1 | AT4G31550 | 51.667 | 60 | 29 | 0 | 169 | 228 | 244 | 303 | 5.22e-17 | 80.9 |
| MS.gene067477.t1 | AT2G44745 | 38.655 | 119 | 68 | 4 | 120 | 236 | 95 | 210 | 5.25e-17 | 79.0 |
| MS.gene067477.t1 | AT5G43290 | 42.857 | 84 | 46 | 2 | 145 | 228 | 89 | 170 | 6.86e-17 | 79.7 |
| MS.gene067477.t1 | AT1G29280 | 50.000 | 60 | 30 | 0 | 172 | 231 | 75 | 134 | 6.88e-17 | 79.3 |
| MS.gene067477.t1 | AT2G34830 | 35.043 | 117 | 70 | 1 | 123 | 233 | 161 | 277 | 7.86e-17 | 80.9 |
| MS.gene067477.t1 | AT2G34830 | 35.043 | 117 | 70 | 1 | 123 | 233 | 170 | 286 | 9.27e-17 | 80.9 |
| MS.gene067477.t1 | AT3G04670 | 51.667 | 60 | 29 | 0 | 169 | 228 | 260 | 319 | 3.01e-16 | 78.6 |
| MS.gene067477.t1 | AT4G01250 | 35.714 | 98 | 63 | 0 | 172 | 269 | 129 | 226 | 3.38e-16 | 78.2 |
| MS.gene067477.t1 | AT4G24240 | 44.304 | 79 | 43 | 1 | 151 | 228 | 260 | 338 | 4.19e-16 | 78.6 |
| MS.gene067477.t1 | AT1G69310 | 48.718 | 78 | 33 | 3 | 151 | 228 | 129 | 199 | 4.66e-16 | 77.4 |
| MS.gene067477.t1 | AT2G37260 | 37.500 | 120 | 64 | 3 | 113 | 228 | 213 | 325 | 5.21e-16 | 78.2 |
| MS.gene067477.t1 | AT2G37260 | 48.000 | 75 | 37 | 2 | 158 | 232 | 72 | 144 | 3.70e-14 | 72.8 |
| MS.gene067477.t1 | AT2G37260 | 37.500 | 120 | 64 | 3 | 113 | 228 | 211 | 323 | 5.28e-16 | 78.2 |
| MS.gene067477.t1 | AT2G37260 | 48.000 | 75 | 37 | 2 | 158 | 232 | 70 | 142 | 3.87e-14 | 72.4 |
| MS.gene067477.t1 | AT2G37260 | 37.037 | 135 | 69 | 4 | 113 | 238 | 293 | 420 | 7.86e-16 | 78.2 |
| MS.gene067477.t1 | AT2G37260 | 48.000 | 75 | 37 | 2 | 158 | 232 | 152 | 224 | 4.19e-14 | 72.8 |
| MS.gene067477.t1 | AT5G28650 | 51.667 | 60 | 29 | 0 | 169 | 228 | 260 | 319 | 7.95e-16 | 77.4 |
| MS.gene067477.t1 | AT1G30650 | 45.714 | 70 | 38 | 0 | 164 | 233 | 210 | 279 | 8.34e-16 | 78.2 |
| MS.gene067477.t1 | AT2G24570 | 50.000 | 60 | 30 | 0 | 169 | 228 | 241 | 300 | 1.40e-15 | 76.6 |
| MS.gene067477.t1 | AT4G23550 | 31.507 | 146 | 88 | 4 | 169 | 314 | 138 | 271 | 2.10e-15 | 75.9 |
| MS.gene067477.t1 | AT3G58710 | 40.323 | 124 | 64 | 3 | 129 | 245 | 24 | 144 | 2.14e-15 | 75.5 |
| MS.gene067477.t1 | AT3G58710 | 40.000 | 125 | 64 | 3 | 129 | 245 | 24 | 145 | 3.05e-15 | 75.1 |
| MS.gene067477.t1 | AT4G30935 | 42.466 | 73 | 41 | 1 | 159 | 231 | 319 | 390 | 4.68e-15 | 75.9 |
| MS.gene067477.t1 | AT5G45050 | 36.667 | 120 | 67 | 2 | 172 | 282 | 1153 | 1272 | 9.42e-15 | 75.5 |
| MS.gene067477.t1 | AT5G45050 | 49.180 | 61 | 31 | 0 | 172 | 232 | 1042 | 1102 | 9.70e-15 | 75.5 |
| MS.gene067477.t1 | AT5G45050 | 36.667 | 120 | 67 | 2 | 172 | 282 | 1181 | 1300 | 9.81e-15 | 75.5 |
| MS.gene067477.t1 | AT2G46130 | 46.835 | 79 | 35 | 3 | 151 | 229 | 12 | 83 | 1.18e-13 | 66.6 |
| MS.gene067477.t1 | AT2G46400 | 41.096 | 73 | 39 | 2 | 156 | 226 | 91 | 161 | 8.93e-12 | 65.1 |
| MS.gene067477.t1 | AT5G45260 | 37.975 | 79 | 47 | 1 | 172 | 248 | 1209 | 1287 | 1.55e-11 | 65.9 |
| MS.gene067477.t1 | AT3G01080 | 40.000 | 75 | 43 | 2 | 172 | 246 | 206 | 278 | 7.45e-11 | 62.8 |
Find 58 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTGTGGTACTTCCATTT+TGG | 0.140306 | 2.4:+553906 | None:intergenic |
| AATGGAAGTACCACAGATTT+TGG | 0.248540 | 2.4:-553902 | MS.gene067477:CDS |
| TCATACTTCTTGAAGCTTAC+AGG | 0.255823 | 2.4:+554880 | None:intergenic |
| GCACTTGCTGCTGCCATATC+AGG | 0.283466 | 2.4:-553832 | MS.gene067477:CDS |
| AATCTAATGGGAGGAGTAAC+TGG | 0.300205 | 2.4:-554494 | MS.gene067477:CDS |
| GGATATCAGTGGAGAAAATA+TGG | 0.332364 | 2.4:-554225 | MS.gene067477:CDS |
| AAGGTTCTACTGGACTAATT+TGG | 0.339982 | 2.4:+554013 | None:intergenic |
| GATGATAAATAATCCCAAAA+TGG | 0.343074 | 2.4:-553920 | MS.gene067477:CDS |
| TTTATCATCATATTCTTAGA+AGG | 0.347068 | 2.4:+553934 | None:intergenic |
| AATAGTAGAAACTTGCATTA+AGG | 0.347618 | 2.4:-554414 | MS.gene067477:CDS |
| CAAGTACAGTTACACTTGAT+TGG | 0.350481 | 2.4:-553981 | MS.gene067477:CDS |
| AAGCAGCAGTAACAATCTAA+TGG | 0.359770 | 2.4:-554507 | MS.gene067477:CDS |
| TTCTTCTTCACTGTGCAGCT+TGG | 0.402986 | 2.4:+554144 | None:intergenic |
| TTCCTACAACGATTGTTTGT+CGG | 0.422479 | 2.4:+554036 | None:intergenic |
| CAAGTTTCTACTATTTGCCT+TGG | 0.425526 | 2.4:+554422 | None:intergenic |
| GGGCTTTGCTCCTTATGATC+AGG | 0.429712 | 2.4:+554557 | None:intergenic |
| GAGAAAGAATCCTGATCATA+AGG | 0.436763 | 2.4:-554567 | MS.gene067477:CDS |
| AGCAGCAGTAACAATCTAAT+GGG | 0.440609 | 2.4:-554506 | MS.gene067477:CDS |
| TCAGTCAAGTGGTGCCTTGG+TGG | 0.446693 | 2.4:-554693 | MS.gene067477:intron |
| TTGAAGTATGCTCTTGGAGA+TGG | 0.459401 | 2.4:+554180 | None:intergenic |
| ATCATGGATTAACACTTCCT+TGG | 0.460598 | 2.4:-554944 | MS.gene067477:CDS |
| GTGGAGAAAATATGGACAAA+AGG | 0.461765 | 2.4:-554217 | MS.gene067477:CDS |
| GAGCATTTGAAGTATGCTCT+TGG | 0.467728 | 2.4:+554174 | None:intergenic |
| CAATCAAGTGTAACTGTACT+TGG | 0.475314 | 2.4:+553982 | None:intergenic |
| AGCAAAGCCCATCTCAGAAA+AGG | 0.487615 | 2.4:-554544 | MS.gene067477:CDS |
| GTCAAGTGGTGCCTTGGTGG+AGG | 0.490012 | 2.4:-554690 | MS.gene067477:CDS |
| TGCTCTGAAGTTAAGATCCT+TGG | 0.493603 | 2.4:+553855 | None:intergenic |
| TTCTTAGAAGGTGCGGAAGA+AGG | 0.509300 | 2.4:+553946 | None:intergenic |
| TCATCCTCATCCTTCTCAAA+CGG | 0.516116 | 2.4:-554061 | MS.gene067477:CDS |
| GGTGCAGAAGAAGGTTCTAC+TGG | 0.520924 | 2.4:+554003 | None:intergenic |
| GCAGAAAACAAGAAGTTGAC+AGG | 0.521855 | 2.4:-554650 | MS.gene067477:CDS |
| ACTGTACTTGGTGCAGAAGA+AGG | 0.524453 | 2.4:+553994 | None:intergenic |
| TATGTTGCAACCACAACTGA+TGG | 0.530178 | 2.4:+554099 | None:intergenic |
| CACATTGAGAAACAATCTGA+TGG | 0.530744 | 2.4:-554597 | MS.gene067477:CDS |
| TGTAAGCTTCAAGAAGTATG+AGG | 0.535043 | 2.4:-554878 | MS.gene067477:CDS |
| CAACATTAATCCACGCAGTA+GGG | 0.544477 | 2.4:-554917 | MS.gene067477:CDS |
| TCAACATTAATCCACGCAGT+AGG | 0.551260 | 2.4:-554918 | MS.gene067477:CDS |
| GTTGTGGTTGCAACATATGA+AGG | 0.552701 | 2.4:-554093 | MS.gene067477:CDS |
| AGCCGACAAACAATCGTTGT+AGG | 0.557166 | 2.4:-554038 | MS.gene067477:CDS |
| TATATCTTCTGCAAAAGAAG+AGG | 0.565925 | 2.4:-554824 | MS.gene067477:intron |
| TTTGTCGGCTCCGTTTGAGA+AGG | 0.566050 | 2.4:+554051 | None:intergenic |
| CATCATATTCTTAGAAGGTG+CGG | 0.576775 | 2.4:+553939 | None:intergenic |
| TGGATTAATGTTGAGATCCA+AGG | 0.586656 | 2.4:+554927 | None:intergenic |
| GAAGAAGGTGCAAAGAAGTG+TGG | 0.601905 | 2.4:-554127 | MS.gene067477:CDS |
| ATATGGACAAAAGGTGACAA+GGG | 0.608161 | 2.4:-554208 | MS.gene067477:CDS |
| ATCAATCATGCAAGAAACCA+AGG | 0.612418 | 2.4:-554439 | MS.gene067477:CDS |
| TACTTCTTGAAGCTTACAGG+AGG | 0.621579 | 2.4:+554883 | None:intergenic |
| AATATGGACAAAAGGTGACA+AGG | 0.623618 | 2.4:-554209 | MS.gene067477:CDS |
| ATCTGTTCCACCAAAATCTG+TGG | 0.633715 | 2.4:+553892 | None:intergenic |
| GGCTCCGTTTGAGAAGGATG+AGG | 0.643319 | 2.4:+554057 | None:intergenic |
| AAGCTTCAAGAAGTATGAGG+TGG | 0.647098 | 2.4:-554875 | MS.gene067477:CDS |
| TTGTGAAAGATGGATATCAG+TGG | 0.648462 | 2.4:-554236 | MS.gene067477:CDS |
| TGTGGATGATCCATCAGTTG+TGG | 0.652865 | 2.4:-554109 | MS.gene067477:CDS |
| AGCAGTAACAATCTAATGGG+AGG | 0.658927 | 2.4:-554503 | MS.gene067477:CDS |
| AAGCTGCACAGTGAAGAAGA+AGG | 0.665099 | 2.4:-554142 | MS.gene067477:CDS |
| GATGGCTACTTCATTGACCA+AGG | 0.672567 | 2.4:-553872 | MS.gene067477:CDS |
| TGTTCATGAACCCTACTGCG+TGG | 0.674456 | 2.4:+554907 | None:intergenic |
| TCGTTTCAGCTCCTCCACCA+AGG | 0.688042 | 2.4:+554679 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAACAATTTTTTCTCATTA+GGG | - | chr2.4:553916-553935 | MS.gene067477:CDS | 15.0% |
| !! | TTATGAATACTAGATATATG+AGG | - | chr2.4:554009-554028 | MS.gene067477:CDS | 20.0% |
| !! | TTTATCATCATATTCTTAGA+AGG | + | chr2.4:554837-554856 | None:intergenic | 20.0% |
| !!! | GAAAACAATTTTTTCTCATT+AGG | - | chr2.4:553915-553934 | MS.gene067477:CDS | 20.0% |
| ! | AATAGTAGAAACTTGCATTA+AGG | - | chr2.4:554354-554373 | MS.gene067477:intron | 25.0% |
| ! | GAAGAAAAATGAATCTTACA+AGG | + | chr2.4:554426-554445 | None:intergenic | 25.0% |
| ! | GATGATAAATAATCCCAAAA+TGG | - | chr2.4:554848-554867 | MS.gene067477:CDS | 25.0% |
| !! | TACTAGATATATGAGGTTTT+TGG | - | chr2.4:554016-554035 | MS.gene067477:CDS | 25.0% |
| !! | TATTGATATTTTCAGTCAAG+TGG | - | chr2.4:554064-554083 | MS.gene067477:CDS | 25.0% |
| !! | TATTTTCAGATTGTGAAAGA+TGG | - | chr2.4:554522-554541 | MS.gene067477:CDS | 25.0% |
| AAAGAAGAGGTAAGTTAGTT+TGG | - | chr2.4:553957-553976 | MS.gene067477:CDS | 30.0% | |
| AGTCATACAACCTAATGAAA+TGG | + | chr2.4:554466-554485 | None:intergenic | 30.0% | |
| ATTTGCAAAGCCATTTCATT+AGG | - | chr2.4:554453-554472 | MS.gene067477:CDS | 30.0% | |
| TATATCTTCTGCAAAAGAAG+AGG | - | chr2.4:553944-553963 | MS.gene067477:CDS | 30.0% | |
| ! | GTAAGTTAGTTTGGTTACTT+TGG | - | chr2.4:553966-553985 | MS.gene067477:CDS | 30.0% |
| ! | GTGTAACATTTTTCCTGATA+TGG | + | chr2.4:554952-554971 | None:intergenic | 30.0% |
| AAGCAGCAGTAACAATCTAA+TGG | - | chr2.4:554261-554280 | MS.gene067477:intron | 35.0% | |
| AAGGTTCTACTGGACTAATT+TGG | + | chr2.4:554758-554777 | None:intergenic | 35.0% | |
| AATATGGACAAAAGGTGACA+AGG | - | chr2.4:554559-554578 | MS.gene067477:CDS | 35.0% | |
| AGCAGCAGTAACAATCTAAT+GGG | - | chr2.4:554262-554281 | MS.gene067477:intron | 35.0% | |
| ATATGGACAAAAGGTGACAA+GGG | - | chr2.4:554560-554579 | MS.gene067477:CDS | 35.0% | |
| ATCAATCATGCAAGAAACCA+AGG | - | chr2.4:554329-554348 | MS.gene067477:intron | 35.0% | |
| CAAGTACAGTTACACTTGAT+TGG | - | chr2.4:554787-554806 | MS.gene067477:intron | 35.0% | |
| CAAGTTTCTACTATTTGCCT+TGG | + | chr2.4:554349-554368 | None:intergenic | 35.0% | |
| CAATCAAGTGTAACTGTACT+TGG | + | chr2.4:554789-554808 | None:intergenic | 35.0% | |
| CACATTGAGAAACAATCTGA+TGG | - | chr2.4:554171-554190 | MS.gene067477:CDS | 35.0% | |
| CATCATATTCTTAGAAGGTG+CGG | + | chr2.4:554832-554851 | None:intergenic | 35.0% | |
| GAGAAAGAATCCTGATCATA+AGG | - | chr2.4:554201-554220 | MS.gene067477:CDS | 35.0% | |
| GGATATCAGTGGAGAAAATA+TGG | - | chr2.4:554543-554562 | MS.gene067477:CDS | 35.0% | |
| GTGGAGAAAATATGGACAAA+AGG | - | chr2.4:554551-554570 | MS.gene067477:CDS | 35.0% | |
| TCATACTTCTTGAAGCTTAC+AGG | + | chr2.4:553891-553910 | None:intergenic | 35.0% | |
| TGGATTAATGTTGAGATCCA+AGG | + | chr2.4:553844-553863 | None:intergenic | 35.0% | |
| TTCCTACAACGATTGTTTGT+CGG | + | chr2.4:554735-554754 | None:intergenic | 35.0% | |
| TTGTGAAAGATGGATATCAG+TGG | - | chr2.4:554532-554551 | MS.gene067477:CDS | 35.0% | |
| ! | AATCTGTGGTACTTCCATTT+TGG | + | chr2.4:554865-554884 | None:intergenic | 35.0% |
| ! | ATCATGGATTAACACTTCCT+TGG | - | chr2.4:553824-553843 | MS.gene067477:CDS | 35.0% |
| ! | ATCTGTGGTACTTCCATTTT+GGG | + | chr2.4:554864-554883 | None:intergenic | 35.0% |
| ! | TGTAAGCTTCAAGAAGTATG+AGG | - | chr2.4:553890-553909 | MS.gene067477:CDS | 35.0% |
| !! | TCAGATTTCCTTTTCTGAGA+TGG | + | chr2.4:554235-554254 | None:intergenic | 35.0% |
| !!! | AATGGAAGTACCACAGATTT+TGG | - | chr2.4:554866-554885 | MS.gene067477:CDS | 35.0% |
| !!! | CAGATTTCCTTTTCTGAGAT+GGG | + | chr2.4:554234-554253 | None:intergenic | 35.0% |
| AATCTAATGGGAGGAGTAAC+TGG | - | chr2.4:554274-554293 | MS.gene067477:intron | 40.0% | |
| AGCAGTAACAATCTAATGGG+AGG | - | chr2.4:554265-554284 | MS.gene067477:intron | 40.0% | |
| ATCTGTTCCACCAAAATCTG+TGG | + | chr2.4:554879-554898 | None:intergenic | 40.0% | |
| CAACATTAATCCACGCAGTA+GGG | - | chr2.4:553851-553870 | MS.gene067477:CDS | 40.0% | |
| GCAGAAAACAAGAAGTTGAC+AGG | - | chr2.4:554118-554137 | MS.gene067477:CDS | 40.0% | |
| GTTGTGGTTGCAACATATGA+AGG | - | chr2.4:554675-554694 | MS.gene067477:CDS | 40.0% | |
| TACTTCTTGAAGCTTACAGG+AGG | + | chr2.4:553888-553907 | None:intergenic | 40.0% | |
| TATGTTGCAACCACAACTGA+TGG | + | chr2.4:554672-554691 | None:intergenic | 40.0% | |
| TCAACATTAATCCACGCAGT+AGG | - | chr2.4:553850-553869 | MS.gene067477:CDS | 40.0% | |
| TCATCCTCATCCTTCTCAAA+CGG | - | chr2.4:554707-554726 | MS.gene067477:intron | 40.0% | |
| TTGAAGTATGCTCTTGGAGA+TGG | + | chr2.4:554591-554610 | None:intergenic | 40.0% | |
| ! | AAGCTTCAAGAAGTATGAGG+TGG | - | chr2.4:553893-553912 | MS.gene067477:CDS | 40.0% |
| ! | GAGCATTTGAAGTATGCTCT+TGG | + | chr2.4:554597-554616 | None:intergenic | 40.0% |
| !! | ACAGATTTTGGTGGAACAGA+TGG | - | chr2.4:554878-554897 | MS.gene067477:CDS | 40.0% |
| !! | TGCTCTGAAGTTAAGATCCT+TGG | + | chr2.4:554916-554935 | None:intergenic | 40.0% |
| AAGCTGCACAGTGAAGAAGA+AGG | - | chr2.4:554626-554645 | MS.gene067477:CDS | 45.0% | |
| AGCAAAGCCCATCTCAGAAA+AGG | - | chr2.4:554224-554243 | MS.gene067477:CDS | 45.0% | |
| AGCCGACAAACAATCGTTGT+AGG | - | chr2.4:554730-554749 | MS.gene067477:intron | 45.0% | |
| GATGGCTACTTCATTGACCA+AGG | - | chr2.4:554896-554915 | MS.gene067477:CDS | 45.0% | |
| TGTGGATGATCCATCAGTTG+TGG | - | chr2.4:554659-554678 | MS.gene067477:CDS | 45.0% | |
| TTCTTCTTCACTGTGCAGCT+TGG | + | chr2.4:554627-554646 | None:intergenic | 45.0% | |
| ! | GAAGAAGGTGCAAAGAAGTG+TGG | - | chr2.4:554641-554660 | MS.gene067477:CDS | 45.0% |
| ! | TTCTTAGAAGGTGCGGAAGA+AGG | + | chr2.4:554825-554844 | None:intergenic | 45.0% |
| ! | TTTTCAGTCAAGTGGTGCCT+TGG | - | chr2.4:554072-554091 | MS.gene067477:CDS | 45.0% |
| !! | ACTGTACTTGGTGCAGAAGA+AGG | + | chr2.4:554777-554796 | None:intergenic | 45.0% |
| !!! | GGAAGTACCACAGATTTTGG+TGG | - | chr2.4:554869-554888 | MS.gene067477:CDS | 45.0% |
| GGGCTTTGCTCCTTATGATC+AGG | + | chr2.4:554214-554233 | None:intergenic | 50.0% | |
| GGTGCAGAAGAAGGTTCTAC+TGG | + | chr2.4:554768-554787 | None:intergenic | 50.0% | |
| TGTTCATGAACCCTACTGCG+TGG | + | chr2.4:553864-553883 | None:intergenic | 50.0% | |
| !! | TTTGTCGGCTCCGTTTGAGA+AGG | + | chr2.4:554720-554739 | None:intergenic | 50.0% |
| GGCTCCGTTTGAGAAGGATG+AGG | + | chr2.4:554714-554733 | None:intergenic | 55.0% | |
| TCAGTCAAGTGGTGCCTTGG+TGG | - | chr2.4:554075-554094 | MS.gene067477:CDS | 55.0% | |
| TCGTTTCAGCTCCTCCACCA+AGG | + | chr2.4:554092-554111 | None:intergenic | 55.0% | |
| ! | GCACTTGCTGCTGCCATATC+AGG | - | chr2.4:554936-554955 | MS.gene067477:CDS | 55.0% |
| GTCAAGTGGTGCCTTGGTGG+AGG | - | chr2.4:554078-554097 | MS.gene067477:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 553810 | 554980 | 553810 | ID=MS.gene067477 |
| chr2.4 | mRNA | 553810 | 554980 | 553810 | ID=MS.gene067477.t1;Parent=MS.gene067477 |
| chr2.4 | exon | 554825 | 554980 | 554825 | ID=MS.gene067477.t1.exon1;Parent=MS.gene067477.t1 |
| chr2.4 | CDS | 554825 | 554980 | 554825 | ID=cds.MS.gene067477.t1;Parent=MS.gene067477.t1 |
| chr2.4 | exon | 554364 | 554711 | 554364 | ID=MS.gene067477.t1.exon2;Parent=MS.gene067477.t1 |
| chr2.4 | CDS | 554364 | 554711 | 554364 | ID=cds.MS.gene067477.t1;Parent=MS.gene067477.t1 |
| chr2.4 | exon | 553810 | 554259 | 553810 | ID=MS.gene067477.t1.exon3;Parent=MS.gene067477.t1 |
| chr2.4 | CDS | 553810 | 554259 | 553810 | ID=cds.MS.gene067477.t1;Parent=MS.gene067477.t1 |
| Gene Sequence |
| Protein sequence |
