AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068197


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068197.t1 MTR_1g050472 99.394 330 2 0 1 330 1 330 0.0 678
MS.gene068197.t1 MTR_0268s0080 96.970 330 10 0 1 330 1 330 0.0 667
MS.gene068197.t1 MTR_7g029110 96.061 330 13 0 1 330 1 330 0.0 641
MS.gene068197.t1 MTR_8g469010 92.727 330 19 1 1 330 1 325 0.0 634
MS.gene068197.t1 MTR_4g117370 76.308 325 76 1 6 330 18 341 0.0 532
MS.gene068197.t1 MTR_6g005270 67.442 43 14 0 265 307 25 67 1.63e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068197.t1 AT3G27060 83.735 332 52 2 1 330 1 332 0.0 558
MS.gene068197.t1 AT3G23580 76.308 325 76 1 6 330 18 341 0.0 518

Find 63 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGAATCGGTCTGGGTTT+GGG 0.237047 1.4:-36600933 None:intergenic
TGCGCATTGGTTGGGATGAA+TGG 0.293239 1.4:+36601636 MS.gene068197:CDS
CGCTTCCTTGCGCATTGGTT+GGG 0.302060 1.4:+36601628 MS.gene068197:CDS
GTTTGTTGCTGATAGGTTAT+TGG 0.303774 1.4:+36601683 MS.gene068197:CDS
AGTGGCGTTCGCCGTCTGTT+AGG 0.311051 1.4:-36601065 None:intergenic
GGGGCAAGAAGGGGTTCTTC+TGG 0.330658 1.4:-36600913 None:intergenic
ATGCAGAATCGGTCTGGGTT+TGG 0.338926 1.4:-36600934 None:intergenic
GCGCATTGGTTGGGATGAAT+GGG 0.350139 1.4:+36601637 MS.gene068197:CDS
AAGCTGATTGGGCGATGAAA+TGG 0.358474 1.4:+36601328 MS.gene068197:CDS
ACAATTTCTTTCACGCGCTC+TGG 0.374811 1.4:-36601564 None:intergenic
TTTGTTGCTGATAGGTTATT+GGG 0.379609 1.4:+36601684 MS.gene068197:CDS
AATCGCTTATATACGTTTCA+AGG 0.387103 1.4:-36601248 None:intergenic
ATGTTCAGAATCCGTTTGAT+TGG 0.390491 1.4:+36601733 MS.gene068197:CDS
ACCGTAGAAGGCACGTGCTT+CGG 0.402800 1.4:-36601178 None:intergenic
GTCTGGGTTTGGGGCAAGAA+GGG 0.415976 1.4:-36600923 None:intergenic
GGGAACATGCAGAATCGGTC+TGG 0.418807 1.4:-36600940 None:intergenic
GCGCTTCCTTGCGCATTGGT+TGG 0.429604 1.4:+36601627 MS.gene068197:CDS
GATTTCTTTCATGAATCTAC+CGG 0.435670 1.4:-36601148 None:intergenic
GTTGCATTTGCTTGTGTTGA+AGG 0.436600 1.4:+36601384 MS.gene068197:CDS
TCAGAATCCGTTTGATTGGA+TGG 0.443597 1.4:+36601737 MS.gene068197:CDS
TCTGGGTTTGGGGCAAGAAG+GGG 0.444554 1.4:-36600922 None:intergenic
AGGCACGTGCTTCGGAGACT+TGG 0.444676 1.4:-36601170 None:intergenic
GCCGTCGGAGGCGGCGAAGA+AGG 0.452615 1.4:-36601106 None:intergenic
CTGTTATGAATAGCTTGAAT+GGG 0.453833 1.4:+36601826 MS.gene068197:CDS
TCTGTTATGAATAGCTTGAA+TGG 0.458759 1.4:+36601825 MS.gene068197:CDS
GAAAATCGCACAGAAACTTC+CGG 0.473049 1.4:-36601418 None:intergenic
GAGGACGATGCCGTCGGAGG+CGG 0.474359 1.4:-36601115 None:intergenic
TGCAATCTGGAAACCGTAGA+AGG 0.475411 1.4:-36601190 None:intergenic
GGCGGTCCAGAATGAGGCTT+CGG 0.476778 1.4:-36601001 None:intergenic
ATGGAATTGATTTCGCTTCA+AGG 0.479094 1.4:+36601756 MS.gene068197:CDS
ATCAATTCCATCCAATCAAA+CGG 0.480364 1.4:-36601744 None:intergenic
CGACGCGCTTCCTTGCGCAT+TGG 0.483293 1.4:+36601623 MS.gene068197:CDS
GAGCTTATTTCACGTGATGA+AGG 0.484978 1.4:+36601486 MS.gene068197:CDS
TCCGAAGCACGTGCCTTCTA+CGG 0.485174 1.4:+36601177 MS.gene068197:CDS
GATAGGTTATTGGGTGAGCT+TGG 0.486660 1.4:+36601693 MS.gene068197:CDS
GGAACATGCAGAATCGGTCT+GGG 0.489835 1.4:-36600939 None:intergenic
CGCATTGGTTGGGATGAATG+GGG 0.491773 1.4:+36601638 MS.gene068197:CDS
ATATTGAGTTTGTTGCTGAT+AGG 0.493329 1.4:+36601676 MS.gene068197:CDS
GGAGGAAAGATCGACTTCTT+CGG 0.499480 1.4:-36601022 None:intergenic
GTGATGCTGTTGAAATTGAA+AGG 0.499878 1.4:+36601589 MS.gene068197:CDS
AGGGGTTCTTCTGGAAAAGC+AGG 0.505225 1.4:-36600904 None:intergenic
GGGTGAGCTTGGTTGTGAAA+AGG 0.505349 1.4:+36601704 MS.gene068197:CDS
GCCTTCTTCGCCGCCTCCGA+CGG 0.505987 1.4:+36601105 MS.gene068197:CDS
GGTCTGGGTTTGGGGCAAGA+AGG 0.509820 1.4:-36600924 None:intergenic
TGGAATTGATTTCGCTTCAA+GGG 0.520571 1.4:+36601757 MS.gene068197:CDS
GGATTGGGAACATGCAGAAT+CGG 0.521690 1.4:-36600945 None:intergenic
TTCTTCGGCGGTCCAGAATG+AGG 0.524296 1.4:-36601007 None:intergenic
GCAGAATCGGTCTGGGTTTG+GGG 0.524980 1.4:-36600932 None:intergenic
TGAAATAAGCTCGTTTGAGA+AGG 0.538441 1.4:-36601475 None:intergenic
TGATGCTGTTGAAATTGAAA+GGG 0.545948 1.4:+36601590 MS.gene068197:CDS
TTTCCTCCGATCTCAAGCAC+TGG 0.566756 1.4:+36601037 MS.gene068197:CDS
TTCGAGGACGATGCCGTCGG+AGG 0.583990 1.4:-36601118 None:intergenic
CGAGGACGTGAGAGATGAAG+TGG 0.584744 1.4:-36601083 None:intergenic
TCGTTTGAGAAGGTTAATCC+CGG 0.587906 1.4:-36601465 None:intergenic
CGGAGGCGGCGAAGAAGGCG+AGG 0.590500 1.4:-36601101 None:intergenic
GCATTGGTTGGGATGAATGG+GGG 0.596821 1.4:+36601639 MS.gene068197:CDS
ATGAATAGCTTGAATGGGAA+TGG 0.603815 1.4:+36601831 MS.gene068197:CDS
TTCATCCCAACCAATGCGCA+AGG 0.604485 1.4:-36601633 None:intergenic
CACTGGCAAAACCTAACAGA+CGG 0.606745 1.4:+36601054 MS.gene068197:CDS
TTGCCAGTGCTTGAGATCGG+AGG 0.610798 1.4:-36601040 None:intergenic
ATCGTCCTCGAAAATCTCGC+CGG 0.639774 1.4:+36601129 MS.gene068197:CDS
GGAAAGATCGACTTCTTCGG+CGG 0.687608 1.4:-36601019 None:intergenic
GAAGAGAGTACATCTCAGAG+TGG 0.694344 1.4:-36601224 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATCCAATACCCAAAAATCT+GGG + chr1.4:36600963-36600982 MS.gene068197:CDS 30.0%
AATCGCTTATATACGTTTCA+AGG - chr1.4:36601251-36601270 None:intergenic 30.0%
ATCAATTCCATCCAATCAAA+CGG - chr1.4:36601747-36601766 None:intergenic 30.0%
CTGTTATGAATAGCTTGAAT+GGG + chr1.4:36601826-36601845 MS.gene068197:CDS 30.0%
GATTTCTTTCATGAATCTAC+CGG - chr1.4:36601151-36601170 None:intergenic 30.0%
TCTGTTATGAATAGCTTGAA+TGG + chr1.4:36601825-36601844 MS.gene068197:CDS 30.0%
TGATGCTGTTGAAATTGAAA+GGG + chr1.4:36601590-36601609 MS.gene068197:CDS 30.0%
TTTGTTGCTGATAGGTTATT+GGG + chr1.4:36601684-36601703 MS.gene068197:CDS 30.0%
! TGTACATTTCCCAGATTTTT+GGG - chr1.4:36600975-36600994 None:intergenic 30.0%
! TTGTACATTTCCCAGATTTT+TGG - chr1.4:36600976-36600995 None:intergenic 30.0%
!! ATATTGAGTTTGTTGCTGAT+AGG + chr1.4:36601676-36601695 MS.gene068197:CDS 30.0%
!!! AGATTTTTGGGTATTGGATT+GGG - chr1.4:36600963-36600982 None:intergenic 30.0%
!!! ATTTTCTGGTTGAAAAAACG+TGG + chr1.4:36601435-36601454 MS.gene068197:CDS 30.0%
AAAAAACGTGGTTTAATGCC+GGG + chr1.4:36601447-36601466 MS.gene068197:CDS 35.0%
AACGCGCTTCTCAAAAAAAT+TGG - chr1.4:36601787-36601806 None:intergenic 35.0%
ATGAATAGCTTGAATGGGAA+TGG + chr1.4:36601831-36601850 MS.gene068197:CDS 35.0%
ATGTTCAGAATCCGTTTGAT+TGG + chr1.4:36601733-36601752 MS.gene068197:CDS 35.0%
CAATCCAATACCCAAAAATC+TGG + chr1.4:36600962-36600981 MS.gene068197:CDS 35.0%
GAAAAAACGTGGTTTAATGC+CGG + chr1.4:36601446-36601465 MS.gene068197:CDS 35.0%
GTGATGCTGTTGAAATTGAA+AGG + chr1.4:36601589-36601608 MS.gene068197:CDS 35.0%
TATCGCGAAAAAAGCTGATT+GGG + chr1.4:36601317-36601336 MS.gene068197:CDS 35.0%
TGAAATAAGCTCGTTTGAGA+AGG - chr1.4:36601478-36601497 None:intergenic 35.0%
! ATGGAATTGATTTCGCTTCA+AGG + chr1.4:36601756-36601775 MS.gene068197:CDS 35.0%
! GTTGAAGGAATCTTTTTCTC+CGG + chr1.4:36601399-36601418 MS.gene068197:CDS 35.0%
! GTTTGTTGCTGATAGGTTAT+TGG + chr1.4:36601683-36601702 MS.gene068197:CDS 35.0%
! TGGAATTGATTTCGCTTCAA+GGG + chr1.4:36601757-36601776 MS.gene068197:CDS 35.0%
!!! AATTTTTTTGAGAAGCGCGT+TGG + chr1.4:36601786-36601805 MS.gene068197:CDS 35.0%
!!! ATTTTTTTGAGAAGCGCGTT+GGG + chr1.4:36601787-36601806 MS.gene068197:CDS 35.0%
!!! CAGATTTTTGGGTATTGGAT+TGG - chr1.4:36600964-36600983 None:intergenic 35.0%
!!! TGCTGATTTTTGCTTCAAGT+TGG + chr1.4:36601857-36601876 MS.gene068197:CDS 35.0%
!!! TTTCCCAGATTTTTGGGTAT+TGG - chr1.4:36600969-36600988 None:intergenic 35.0%
GAAAATCGCACAGAAACTTC+CGG - chr1.4:36601421-36601440 None:intergenic 40.0%
GAGCTTATTTCACGTGATGA+AGG + chr1.4:36601486-36601505 MS.gene068197:CDS 40.0%
GTATCGCGAAAAAAGCTGAT+TGG + chr1.4:36601316-36601335 MS.gene068197:CDS 40.0%
GTTGCATTTGCTTGTGTTGA+AGG + chr1.4:36601384-36601403 MS.gene068197:CDS 40.0%
TCAGAATCCGTTTGATTGGA+TGG + chr1.4:36601737-36601756 MS.gene068197:CDS 40.0%
TCGTTTGAGAAGGTTAATCC+CGG - chr1.4:36601468-36601487 None:intergenic 40.0%
! GAAGTTTCTGTGCGATTTTC+TGG + chr1.4:36601421-36601440 MS.gene068197:CDS 40.0%
! GGATGTTTTCGATTGCAATC+TGG - chr1.4:36601206-36601225 None:intergenic 40.0%
!!! TCAGCTTTTTTCGCGATACA+TGG - chr1.4:36601315-36601334 None:intergenic 40.0%
!!! TTTTTTTGAGAAGCGCGTTG+GGG + chr1.4:36601788-36601807 MS.gene068197:CDS 40.0%
AAAAAGCCGAAGCCTCATTC+TGG + chr1.4:36600995-36601014 MS.gene068197:CDS 45.0%
AAGCTGATTGGGCGATGAAA+TGG + chr1.4:36601328-36601347 MS.gene068197:CDS 45.0%
ACAATTTCTTTCACGCGCTC+TGG - chr1.4:36601567-36601586 None:intergenic 45.0%
CACTGGCAAAACCTAACAGA+CGG + chr1.4:36601054-36601073 MS.gene068197:CDS 45.0%
GATAGGTTATTGGGTGAGCT+TGG + chr1.4:36601693-36601712 MS.gene068197:CDS 45.0%
GGATTGGGAACATGCAGAAT+CGG - chr1.4:36600948-36600967 None:intergenic 45.0%
TGCAATCTGGAAACCGTAGA+AGG - chr1.4:36601193-36601212 None:intergenic 45.0%
! GAAGAGAGTACATCTCAGAG+TGG - chr1.4:36601227-36601246 None:intergenic 45.0%
! GGAGGAAAGATCGACTTCTT+CGG - chr1.4:36601025-36601044 None:intergenic 45.0%
! GTTTTGCCAGTGCTTGAGAT+CGG - chr1.4:36601046-36601065 None:intergenic 45.0%
ATCGTCCTCGAAAATCTCGC+CGG + chr1.4:36601129-36601148 MS.gene068197:CDS 50.0%
ATGCAGAATCGGTCTGGGTT+TGG - chr1.4:36600937-36600956 None:intergenic 50.0%
GGAACATGCAGAATCGGTCT+GGG - chr1.4:36600942-36600961 None:intergenic 50.0%
GGGTGAGCTTGGTTGTGAAA+AGG + chr1.4:36601704-36601723 MS.gene068197:CDS 50.0%
TGCAGAATCGGTCTGGGTTT+GGG - chr1.4:36600936-36600955 None:intergenic 50.0%
TTCATCCCAACCAATGCGCA+AGG - chr1.4:36601636-36601655 None:intergenic 50.0%
TTTCCTCCGATCTCAAGCAC+TGG + chr1.4:36601037-36601056 MS.gene068197:CDS 50.0%
! AGGGGTTCTTCTGGAAAAGC+AGG - chr1.4:36600907-36600926 None:intergenic 50.0%
! ATCTACCGGCGAGATTTTCG+AGG - chr1.4:36601137-36601156 None:intergenic 50.0%
! GGAAAGATCGACTTCTTCGG+CGG - chr1.4:36601022-36601041 None:intergenic 50.0%
!! ATTTTCGAGGACGATGCCGT+CGG - chr1.4:36601124-36601143 None:intergenic 50.0%
!! CGCATTGGTTGGGATGAATG+GGG + chr1.4:36601638-36601657 MS.gene068197:CDS 50.0%
!! GCATTGGTTGGGATGAATGG+GGG + chr1.4:36601639-36601658 MS.gene068197:CDS 50.0%
!! GCGCATTGGTTGGGATGAAT+GGG + chr1.4:36601637-36601656 MS.gene068197:CDS 50.0%
!! TGCGCATTGGTTGGGATGAA+TGG + chr1.4:36601636-36601655 MS.gene068197:CDS 50.0%
CGAGGACGTGAGAGATGAAG+TGG - chr1.4:36601086-36601105 None:intergenic 55.0%
GCAGAATCGGTCTGGGTTTG+GGG - chr1.4:36600935-36600954 None:intergenic 55.0%
GGGAACATGCAGAATCGGTC+TGG - chr1.4:36600943-36600962 None:intergenic 55.0%
TCCGAAGCACGTGCCTTCTA+CGG + chr1.4:36601177-36601196 MS.gene068197:CDS 55.0%
TTCTTCGGCGGTCCAGAATG+AGG - chr1.4:36601010-36601029 None:intergenic 55.0%
TTGCCAGTGCTTGAGATCGG+AGG - chr1.4:36601043-36601062 None:intergenic 55.0%
! ACCGTAGAAGGCACGTGCTT+CGG - chr1.4:36601181-36601200 None:intergenic 55.0%
! GTCTGGGTTTGGGGCAAGAA+GGG - chr1.4:36600926-36600945 None:intergenic 55.0%
! TCTGGGTTTGGGGCAAGAAG+GGG - chr1.4:36600925-36600944 None:intergenic 55.0%
!! CGCTTCCTTGCGCATTGGTT+GGG + chr1.4:36601628-36601647 MS.gene068197:CDS 55.0%
AGGCACGTGCTTCGGAGACT+TGG - chr1.4:36601173-36601192 None:intergenic 60.0%
AGTGGCGTTCGCCGTCTGTT+AGG - chr1.4:36601068-36601087 None:intergenic 60.0%
GGCGGTCCAGAATGAGGCTT+CGG - chr1.4:36601004-36601023 None:intergenic 60.0%
GGGGCAAGAAGGGGTTCTTC+TGG - chr1.4:36600916-36600935 None:intergenic 60.0%
! GCGCTTCCTTGCGCATTGGT+TGG + chr1.4:36601627-36601646 MS.gene068197:CDS 60.0%
! GGTCTGGGTTTGGGGCAAGA+AGG - chr1.4:36600927-36600946 None:intergenic 60.0%
CGACGCGCTTCCTTGCGCAT+TGG + chr1.4:36601623-36601642 MS.gene068197:CDS 65.0%
TTCGAGGACGATGCCGTCGG+AGG - chr1.4:36601121-36601140 None:intergenic 65.0%
GAGGACGATGCCGTCGGAGG+CGG - chr1.4:36601118-36601137 None:intergenic 70.0%
GCCTTCTTCGCCGCCTCCGA+CGG + chr1.4:36601105-36601124 MS.gene068197:CDS 70.0%
CGGAGGCGGCGAAGAAGGCG+AGG - chr1.4:36601104-36601123 None:intergenic 75.0%
GCCGTCGGAGGCGGCGAAGA+AGG - chr1.4:36601109-36601128 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 36600901 36601893 36600901 ID=MS.gene068197
chr1.4 mRNA 36600901 36601893 36600901 ID=MS.gene068197.t1;Parent=MS.gene068197
chr1.4 exon 36600901 36601893 36600901 ID=MS.gene068197.t1.exon1;Parent=MS.gene068197.t1
chr1.4 CDS 36600901 36601893 36600901 ID=cds.MS.gene068197.t1;Parent=MS.gene068197.t1
Gene Sequence

>MS.gene068197

ATGCCTGCTTTTCCAGAAGAACCCCTTCTTGCCCCAAACCCAGACCGATTCTGCATGTTCCCAATCCAATACCCAAAAATCTGGGAAATGTACAAAAAAGCCGAAGCCTCATTCTGGACCGCCGAAGAAGTCGATCTTTCCTCCGATCTCAAGCACTGGCAAAACCTAACAGACGGCGAACGCCACTTCATCTCTCACGTCCTCGCCTTCTTCGCCGCCTCCGACGGCATCGTCCTCGAAAATCTCGCCGGTAGATTCATGAAAGAAATCCAAGTCTCCGAAGCACGTGCCTTCTACGGTTTCCAGATTGCAATCGAAAACATCCACTCTGAGATGTACTCTCTTCTCCTTGAAACGTATATAAGCGATTCTGCTGAAAAGAATCGCTTATTTCATGCAATTGAAACAATTCCATGTATCGCGAAAAAAGCTGATTGGGCGATGAAATGGATCGATTCAAGCGATTCTTTTGCAGAAAGAATCGTTGCATTTGCTTGTGTTGAAGGAATCTTTTTCTCCGGAAGTTTCTGTGCGATTTTCTGGTTGAAAAAACGTGGTTTAATGCCGGGATTAACCTTCTCAAACGAGCTTATTTCACGTGATGAAGGATTACACTGTGATTTCGCTTGTTTGTTATACTCACTTCTACGCAAGAAGCTAAGTCCAGAGCGCGTGAAAGAAATTGTTTGTGATGCTGTTGAAATTGAAAGGGAGTTTGTGTGCGACGCGCTTCCTTGCGCATTGGTTGGGATGAATGGGGGTTTGATGAGTACGTATATTGAGTTTGTTGCTGATAGGTTATTGGGTGAGCTTGGTTGTGAAAAGGTTTACAATGTTCAGAATCCGTTTGATTGGATGGAATTGATTTCGCTTCAAGGGAAAACCAATTTTTTTGAGAAGCGCGTTGGGGAGTATCAGAAAGCTTCTGTTATGAATAGCTTGAATGGGAATGGTGCTGCTGATTTTTGCTTCAAGTTGGATGAAGATTTTTAG

Protein sequence

>MS.gene068197.t1

MPAFPEEPLLAPNPDRFCMFPIQYPKIWEMYKKAEASFWTAEEVDLSSDLKHWQNLTDGERHFISHVLAFFAASDGIVLENLAGRFMKEIQVSEARAFYGFQIAIENIHSEMYSLLLETYISDSAEKNRLFHAIETIPCIAKKADWAMKWIDSSDSFAERIVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSPERVKEIVCDAVEIEREFVCDALPCALVGMNGGLMSTYIEFVADRLLGELGCEKVYNVQNPFDWMELISLQGKTNFFEKRVGEYQKASVMNSLNGNGAADFCFKLDEDF