Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07156.t1 | XP_003591578.1 | 94.2 | 464 | 27 | 0 | 1 | 464 | 1 | 464 | 7.40E-196 | 693.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07156.t1 | Q9XZC0 | 33.0 | 115 | 69 | 4 | 167 | 278 | 1060 | 1169 | 2.9e-07 | 58.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07156.t1 | D3J163 | 94.2 | 464 | 27 | 0 | 1 | 464 | 1 | 464 | 5.4e-196 | 693.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene07156 | -0.849462 | 3.18E-60 | -1.69E-46 |
| MS.gene049222 | MS.gene07156 | -0.804084 | 2.56E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene07156 | -0.808464 | 3.06E-50 | -1.69E-46 |
| MS.gene049425 | MS.gene07156 | 0.804285 | 2.32E-49 | -1.69E-46 |
| MS.gene049627 | MS.gene07156 | -0.818294 | 2.11E-52 | -1.69E-46 |
| MS.gene049634 | MS.gene07156 | 0.815694 | 8.11E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene07156 | -0.826394 | 2.78E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene07156 | -0.820876 | 5.44E-53 | -1.69E-46 |
| MS.gene050173 | MS.gene07156 | 0.809544 | 1.80E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene07156 | -0.807937 | 3.96E-50 | -1.69E-46 |
| MS.gene050353 | MS.gene07156 | 0.814515 | 1.48E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene07156 | 0.824011 | 1.02E-53 | -1.69E-46 |
| MS.gene050475 | MS.gene07156 | 0.814532 | 1.47E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene07156 | -0.8159 | 7.29E-52 | -1.69E-46 |
| MS.gene050518 | MS.gene07156 | -0.80538 | 1.37E-49 | -1.69E-46 |
| MS.gene050541 | MS.gene07156 | 0.811508 | 6.76E-51 | -1.69E-46 |
| MS.gene050612 | MS.gene07156 | -0.800755 | 1.24E-48 | -1.69E-46 |
| MS.gene05079 | MS.gene07156 | 0.813771 | 2.16E-51 | -1.69E-46 |
| MS.gene051127 | MS.gene07156 | 0.81264 | 3.83E-51 | -1.69E-46 |
| MS.gene051364 | MS.gene07156 | 0.810462 | 1.14E-50 | -1.69E-46 |
| MS.gene051366 | MS.gene07156 | 0.81197 | 5.36E-51 | -1.69E-46 |
| MS.gene051566 | MS.gene07156 | -0.800295 | 1.54E-48 | -1.69E-46 |
| MS.gene051694 | MS.gene07156 | -0.826714 | 2.33E-54 | -1.69E-46 |
| MS.gene051858 | MS.gene07156 | -0.848142 | 7.40E-60 | -1.69E-46 |
| MS.gene052379 | MS.gene07156 | -0.822734 | 2.02E-53 | -1.69E-46 |
| MS.gene053420 | MS.gene07156 | 0.83377 | 4.41E-56 | -1.69E-46 |
| MS.gene053561 | MS.gene07156 | 0.857936 | 1.16E-62 | -1.69E-46 |
| MS.gene054642 | MS.gene07156 | -0.805536 | 1.27E-49 | -1.69E-46 |
| MS.gene054708 | MS.gene07156 | 0.800626 | 1.32E-48 | -1.69E-46 |
| MS.gene055124 | MS.gene07156 | 0.815896 | 7.31E-52 | -1.69E-46 |
| MS.gene055154 | MS.gene07156 | 0.848235 | 6.97E-60 | -1.69E-46 |
| MS.gene055202 | MS.gene07156 | 0.825996 | 3.46E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene07156 | -0.821934 | 3.10E-53 | -1.69E-46 |
| MS.gene055638 | MS.gene07156 | -0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene056550 | MS.gene07156 | 0.808629 | 2.82E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene07156 | -0.806003 | 1.02E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene07156 | 0.806598 | 7.61E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene07156 | 0.806567 | 7.73E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene07156 | -0.800717 | 1.26E-48 | -1.69E-46 |
| MS.gene058773 | MS.gene07156 | -0.806272 | 8.92E-50 | -1.69E-46 |
| MS.gene058840 | MS.gene07156 | 0.832807 | 7.66E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene07156 | -0.814977 | 1.17E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene07156 | -0.813429 | 2.57E-51 | -1.69E-46 |
| MS.gene059902 | MS.gene07156 | -0.856428 | 3.24E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07156.t1 | MTR_1g089180 | 94.181 | 464 | 27 | 0 | 1 | 464 | 1 | 464 | 0.0 | 910 |
| MS.gene07156.t1 | MTR_6g027840 | 29.825 | 456 | 290 | 8 | 1 | 429 | 1 | 453 | 7.30e-54 | 189 |
| MS.gene07156.t1 | MTR_2g045090 | 86.567 | 67 | 9 | 0 | 183 | 249 | 9 | 75 | 6.12e-32 | 119 |
| MS.gene07156.t1 | MTR_5g043640 | 32.609 | 184 | 98 | 4 | 250 | 429 | 51 | 212 | 1.29e-18 | 85.5 |
| MS.gene07156.t1 | MTR_5g043640 | 30.729 | 192 | 110 | 3 | 220 | 410 | 57 | 226 | 1.28e-15 | 76.6 |
| MS.gene07156.t1 | MTR_8g056020 | 26.000 | 350 | 211 | 11 | 1 | 306 | 3 | 348 | 2.53e-16 | 80.5 |
| MS.gene07156.t1 | MTR_2g096760 | 31.250 | 224 | 137 | 8 | 211 | 429 | 7 | 218 | 1.83e-14 | 75.5 |
| MS.gene07156.t1 | MTR_1g112830 | 27.933 | 179 | 109 | 2 | 257 | 435 | 173 | 331 | 1.90e-14 | 76.3 |
| MS.gene07156.t1 | MTR_2g006870 | 27.692 | 195 | 121 | 3 | 228 | 402 | 501 | 695 | 6.80e-14 | 74.7 |
| MS.gene07156.t1 | MTR_4g062100 | 28.016 | 257 | 151 | 11 | 183 | 429 | 66 | 298 | 1.31e-13 | 73.2 |
| MS.gene07156.t1 | MTR_2g096190 | 25.704 | 284 | 173 | 10 | 161 | 429 | 34 | 294 | 1.40e-13 | 73.2 |
| MS.gene07156.t1 | MTR_4g063830 | 28.384 | 229 | 149 | 5 | 206 | 429 | 1 | 219 | 1.95e-13 | 72.4 |
| MS.gene07156.t1 | MTR_4g116160 | 28.235 | 170 | 102 | 2 | 260 | 429 | 77 | 226 | 2.29e-13 | 72.4 |
| MS.gene07156.t1 | MTR_4g129470 | 27.826 | 230 | 141 | 6 | 221 | 429 | 20 | 245 | 3.62e-13 | 72.0 |
| MS.gene07156.t1 | MTR_1g100797 | 28.500 | 200 | 116 | 5 | 255 | 429 | 47 | 244 | 5.14e-13 | 71.2 |
| MS.gene07156.t1 | MTR_4g116160 | 28.235 | 170 | 102 | 2 | 260 | 429 | 77 | 226 | 5.33e-13 | 71.6 |
| MS.gene07156.t1 | MTR_7g100430 | 28.512 | 242 | 135 | 7 | 213 | 444 | 119 | 332 | 5.35e-13 | 71.6 |
| MS.gene07156.t1 | MTR_0020s0130 | 27.803 | 223 | 131 | 8 | 233 | 429 | 26 | 244 | 1.09e-12 | 70.5 |
| MS.gene07156.t1 | MTR_4g021880 | 26.457 | 223 | 149 | 5 | 220 | 437 | 100 | 312 | 2.30e-12 | 68.9 |
| MS.gene07156.t1 | MTR_4g021880 | 29.858 | 211 | 129 | 5 | 221 | 425 | 136 | 333 | 1.84e-11 | 66.2 |
| MS.gene07156.t1 | MTR_4g092760 | 29.808 | 208 | 108 | 5 | 268 | 458 | 72 | 258 | 2.33e-12 | 69.3 |
| MS.gene07156.t1 | MTR_4g021880 | 26.457 | 223 | 149 | 5 | 220 | 437 | 100 | 312 | 4.02e-12 | 68.6 |
| MS.gene07156.t1 | MTR_4g021880 | 30.332 | 211 | 128 | 7 | 221 | 425 | 136 | 333 | 1.41e-11 | 67.0 |
| MS.gene07156.t1 | MTR_3g088410 | 36.735 | 98 | 62 | 0 | 342 | 439 | 22 | 119 | 1.23e-11 | 63.2 |
| MS.gene07156.t1 | MTR_7g117620 | 31.551 | 187 | 109 | 6 | 249 | 417 | 112 | 297 | 2.29e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07156.t1 | AT2G03430 | 31.551 | 187 | 104 | 2 | 246 | 429 | 40 | 205 | 4.31e-17 | 80.9 |
| MS.gene07156.t1 | AT2G03430 | 28.571 | 231 | 114 | 5 | 180 | 410 | 40 | 219 | 1.84e-14 | 73.2 |
| MS.gene07156.t1 | AT5G60070 | 28.309 | 272 | 153 | 8 | 194 | 429 | 39 | 304 | 1.29e-16 | 82.8 |
| MS.gene07156.t1 | AT2G14255 | 30.682 | 176 | 101 | 3 | 260 | 435 | 65 | 219 | 2.20e-15 | 79.0 |
| MS.gene07156.t1 | AT2G14255 | 25.581 | 215 | 133 | 4 | 214 | 425 | 55 | 245 | 4.17e-14 | 75.1 |
| MS.gene07156.t1 | AT5G20350 | 30.000 | 170 | 99 | 2 | 260 | 429 | 72 | 221 | 1.54e-14 | 76.6 |
| MS.gene07156.t1 | AT2G28840 | 27.500 | 240 | 120 | 7 | 200 | 430 | 25 | 219 | 1.97e-14 | 75.5 |
| MS.gene07156.t1 | AT2G31820 | 29.520 | 271 | 159 | 10 | 187 | 429 | 151 | 417 | 2.85e-14 | 75.9 |
| MS.gene07156.t1 | AT2G31820 | 28.151 | 238 | 149 | 9 | 218 | 434 | 152 | 388 | 1.69e-12 | 70.1 |
| MS.gene07156.t1 | AT1G07710 | 27.734 | 256 | 153 | 9 | 183 | 429 | 62 | 294 | 3.80e-14 | 75.1 |
| MS.gene07156.t1 | AT1G07710 | 27.734 | 256 | 153 | 9 | 183 | 429 | 62 | 294 | 3.80e-14 | 75.1 |
| MS.gene07156.t1 | AT1G05640 | 31.081 | 296 | 168 | 13 | 167 | 429 | 89 | 381 | 6.91e-14 | 74.3 |
| MS.gene07156.t1 | AT5G57740 | 29.412 | 204 | 119 | 6 | 255 | 437 | 53 | 252 | 2.85e-13 | 72.4 |
| MS.gene07156.t1 | AT3G04710 | 33.149 | 181 | 95 | 6 | 257 | 435 | 283 | 439 | 6.91e-13 | 71.2 |
| MS.gene07156.t1 | AT3G04710 | 27.309 | 249 | 151 | 6 | 181 | 425 | 273 | 495 | 9.46e-11 | 64.7 |
| MS.gene07156.t1 | AT3G04710 | 33.149 | 181 | 95 | 6 | 257 | 435 | 59 | 215 | 1.08e-12 | 70.1 |
| MS.gene07156.t1 | AT3G04710 | 33.149 | 181 | 95 | 6 | 257 | 435 | 59 | 215 | 1.19e-12 | 70.1 |
| MS.gene07156.t1 | AT5G02620 | 28.163 | 245 | 133 | 8 | 194 | 421 | 24 | 242 | 1.34e-12 | 70.1 |
| MS.gene07156.t1 | AT5G02620 | 28.163 | 245 | 133 | 8 | 194 | 421 | 24 | 242 | 1.34e-12 | 70.1 |
| MS.gene07156.t1 | AT5G02620 | 26.384 | 307 | 183 | 12 | 161 | 449 | 24 | 305 | 6.67e-12 | 68.2 |
| MS.gene07156.t1 | AT5G02620 | 26.384 | 307 | 183 | 12 | 161 | 449 | 24 | 305 | 6.67e-12 | 68.2 |
| MS.gene07156.t1 | AT5G02620 | 26.384 | 307 | 183 | 12 | 161 | 449 | 24 | 305 | 6.67e-12 | 68.2 |
| MS.gene07156.t1 | AT2G26650 | 29.714 | 175 | 100 | 3 | 244 | 418 | 437 | 588 | 1.79e-11 | 67.0 |
| MS.gene07156.t1 | AT2G26650 | 28.571 | 175 | 102 | 2 | 244 | 418 | 542 | 693 | 2.27e-11 | 66.6 |
| MS.gene07156.t1 | AT5G07270 | 28.643 | 199 | 111 | 5 | 255 | 429 | 47 | 238 | 3.82e-11 | 65.5 |
| MS.gene07156.t1 | AT5G14230 | 27.778 | 306 | 186 | 12 | 153 | 429 | 303 | 602 | 4.86e-11 | 65.5 |
| MS.gene07156.t1 | AT5G12320 | 31.731 | 104 | 71 | 0 | 336 | 439 | 7 | 110 | 5.12e-11 | 61.2 |
| MS.gene07156.t1 | AT5G14230 | 27.778 | 306 | 186 | 12 | 153 | 429 | 143 | 442 | 7.75e-11 | 64.7 |
Find 91 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATACAAAGGTGGTTGATTA+AGG | 0.247464 | 1.4:+70980601 | None:intergenic |
| CTGAAGCTTCTTGCAGCTAA+TGG | 0.286643 | 1.4:-70980210 | MS.gene07156:CDS |
| CTTGTTTGGTGTACTACATT+GGG | 0.316912 | 1.4:-70979818 | MS.gene07156:CDS |
| ATCGTCTGTAGTGGCTTTAC+CGG | 0.321963 | 1.4:+70980536 | None:intergenic |
| TGAATCTTTCAGCGACGAAT+TGG | 0.365290 | 1.4:-70980106 | MS.gene07156:CDS |
| ATTCCGTTTGCAATAAGAAC+AGG | 0.367201 | 1.4:-70980246 | MS.gene07156:CDS |
| CACGCGCTGAAGAAGTGTTT+AGG | 0.372386 | 1.4:-70979757 | MS.gene07156:CDS |
| TTGCATTGTGCTGCTGAAGC+TGG | 0.388322 | 1.4:-70979589 | MS.gene07156:CDS |
| AGTGGTCACACGCGCTTGTT+TGG | 0.388536 | 1.4:-70979832 | MS.gene07156:CDS |
| TGCAGTTATAACCGTCGTTT+TGG | 0.395549 | 1.4:-70980469 | MS.gene07156:CDS |
| TGTTGGGTTTGTTGTTGTTA+AGG | 0.397778 | 1.4:+70980698 | None:intergenic |
| GCTTGTTTGGTGTACTACAT+TGG | 0.398911 | 1.4:-70979819 | MS.gene07156:CDS |
| TCACTCGCTGCCACGTGAAC+TGG | 0.399901 | 1.4:+70980051 | None:intergenic |
| CGACGAATTGGATGTGAATT+CGG | 0.401629 | 1.4:-70980094 | MS.gene07156:CDS |
| GAGTGGTGGGATTACTGATA+AGG | 0.420907 | 1.4:+70980656 | None:intergenic |
| TTGTTGATTGAAAAGGTTGT+TGG | 0.422808 | 1.4:+70980681 | None:intergenic |
| TTCCCTGTTCTTATTGCAAA+CGG | 0.423986 | 1.4:+70980243 | None:intergenic |
| AGTGGTGGGATTACTGATAA+GGG | 0.424185 | 1.4:+70980657 | None:intergenic |
| ACGCGCTGAAGAAGTGTTTA+GGG | 0.432430 | 1.4:-70979756 | MS.gene07156:CDS |
| TGTTGATTGAAAAGGTTGTT+GGG | 0.435252 | 1.4:+70980682 | None:intergenic |
| CTACATTGGGCTTCATTCAA+AGG | 0.464048 | 1.4:-70979688 | MS.gene07156:CDS |
| TAAGGGTTTGTTGATTGAAA+AGG | 0.465974 | 1.4:+70980674 | None:intergenic |
| TGAAACTCGCGGTTGAAAGC+GGG | 0.467803 | 1.4:-70980344 | MS.gene07156:CDS |
| ATGAAACTCGCGGTTGAAAG+CGG | 0.472267 | 1.4:-70980345 | MS.gene07156:CDS |
| TGGGTGGAGCCCACTACATT+GGG | 0.483776 | 1.4:-70979701 | MS.gene07156:CDS |
| TTGAATGAAGCCCAATGTAG+TGG | 0.486452 | 1.4:+70979691 | None:intergenic |
| TACATTGGGCTTCATTCAAA+GGG | 0.487037 | 1.4:-70979687 | MS.gene07156:CDS |
| GGCTTTACCGGTTGGTAACA+TGG | 0.487305 | 1.4:+70980548 | None:intergenic |
| TGGACATTTACAAGTTGCAT+TGG | 0.499069 | 1.4:-70979569 | MS.gene07156:CDS |
| CGTCGTCTCTTTAACAAACC+TGG | 0.511564 | 1.4:-70980510 | MS.gene07156:CDS |
| TCTGTAGTGGCTTTACCGGT+TGG | 0.516796 | 1.4:+70980540 | None:intergenic |
| ATGGGTGGAGCCCACTACAT+TGG | 0.517641 | 1.4:-70979702 | MS.gene07156:CDS |
| CTGAAGAAGTGTTTAGGGGA+AGG | 0.519433 | 1.4:-70979751 | MS.gene07156:CDS |
| GTTGATAGTGTTGATGATGC+TGG | 0.521848 | 1.4:-70979622 | MS.gene07156:CDS |
| TTTGCACGAAGCTGCAACAA+TGG | 0.526496 | 1.4:-70980157 | MS.gene07156:CDS |
| TCACGGAAAACGTGGGGACC+AGG | 0.526974 | 1.4:+70980492 | None:intergenic |
| GAAGAAGTGTTTAGGGGAAG+GGG | 0.527190 | 1.4:-70979749 | MS.gene07156:CDS |
| TGAATAGTAAAGATCAGAAT+GGG | 0.527705 | 1.4:-70979720 | MS.gene07156:CDS |
| CGAACGGTGATGACGTCAGC+AGG | 0.533305 | 1.4:+70980576 | None:intergenic |
| CGAACCTCCATGTTACCAAC+CGG | 0.541438 | 1.4:-70980555 | MS.gene07156:CDS |
| ACCGATCTTATCGCCGCCGG+TGG | 0.542266 | 1.4:-70980306 | MS.gene07156:CDS |
| CATCACCACCATGGATAGGT+TGG | 0.547063 | 1.4:-70980826 | None:intergenic |
| GTGAATAGTAAAGATCAGAA+TGG | 0.548627 | 1.4:-70979721 | MS.gene07156:CDS |
| TGAAGAAGTGTTTAGGGGAA+GGG | 0.558826 | 1.4:-70979750 | MS.gene07156:CDS |
| TTCGACGCTGCATGATGAAG+TGG | 0.565036 | 1.4:+70979952 | None:intergenic |
| AAATACGCAAGAGAAATAAA+CGG | 0.568319 | 1.4:-70979883 | MS.gene07156:CDS |
| TCTAAAATTCACATCTCCAC+CGG | 0.573420 | 1.4:+70980290 | None:intergenic |
| TAACTGCATCACGGAAAACG+TGG | 0.574738 | 1.4:+70980484 | None:intergenic |
| GAAGTGTTTAGGGGAAGGGG+CGG | 0.580140 | 1.4:-70979746 | MS.gene07156:CDS |
| TCCACCGGCGGCGATAAGAT+CGG | 0.585692 | 1.4:+70980305 | None:intergenic |
| TAACCATCACCACCATGGAT+AGG | 0.591904 | 1.4:-70980830 | None:intergenic |
| TTCCGTTTGCAATAAGAACA+GGG | 0.592729 | 1.4:-70980245 | MS.gene07156:CDS |
| ATGTTGATGAAGAGAGTGGT+GGG | 0.599092 | 1.4:+70980643 | None:intergenic |
| TTTGTTGGAACATGGTGCTG+AGG | 0.600880 | 1.4:-70979644 | MS.gene07156:CDS |
| TTGTTTGGTGTACTACATTG+GGG | 0.602036 | 1.4:-70979817 | MS.gene07156:CDS |
| GTTCACGTGGCAGCGAGTGA+AGG | 0.604991 | 1.4:-70980048 | MS.gene07156:CDS |
| GCTTCTACGAGCGGCGCGTG+TGG | 0.605091 | 1.4:-70979788 | MS.gene07156:CDS |
| TGAATGAAGCCCAATGTAGT+GGG | 0.611394 | 1.4:+70979692 | None:intergenic |
| AACAGCGTTTGAAATAGCAT+CGG | 0.612401 | 1.4:-70979857 | MS.gene07156:CDS |
| GCTTCAGCAGCACAATGCAA+AGG | 0.613566 | 1.4:+70979592 | None:intergenic |
| GATAACAAACTCAGCGACGG+TGG | 0.615331 | 1.4:+70980440 | None:intergenic |
| AACTGCATCACGGAAAACGT+GGG | 0.618543 | 1.4:+70980485 | None:intergenic |
| TGGTAACATGGAGGTTCGAA+CGG | 0.618785 | 1.4:+70980560 | None:intergenic |
| TGTCCACCGTCGAGAATCAA+CGG | 0.621480 | 1.4:+70979975 | None:intergenic |
| GGGCGACGCGCTTCTACGAG+CGG | 0.623068 | 1.4:-70979797 | MS.gene07156:CDS |
| GTCACCGATCTTATCGCCGC+CGG | 0.623931 | 1.4:-70980309 | MS.gene07156:CDS |
| TTTGAAATAGCATCGGAGAG+TGG | 0.632198 | 1.4:-70979850 | MS.gene07156:CDS |
| ATGCAGAATCAACGATTCAG+TGG | 0.632543 | 1.4:-70980187 | MS.gene07156:CDS |
| AGGGAGAATAAAGAGTGTCA+AGG | 0.637144 | 1.4:-70979668 | MS.gene07156:CDS |
| CGCGCTGAAGAAGTGTTTAG+GGG | 0.639372 | 1.4:-70979755 | MS.gene07156:CDS |
| ATAGTAAAGATCAGAATGGG+TGG | 0.645517 | 1.4:-70979717 | MS.gene07156:CDS |
| ACGACGGAGATCGTCTGTAG+TGG | 0.650285 | 1.4:+70980527 | None:intergenic |
| TAAGGAAACTGCAACAGACA+TGG | 0.651639 | 1.4:+70980716 | None:intergenic |
| GAGGTTTGTGAGCTTGAACG+AGG | 0.657050 | 1.4:+70980746 | None:intergenic |
| CAGGTTTGTTAAAGAGACGA+CGG | 0.657239 | 1.4:+70980511 | None:intergenic |
| ACGTCAGCAGGTTCATACAA+AGG | 0.662183 | 1.4:+70980588 | None:intergenic |
| AATGATGACACCAGTTCACG+TGG | 0.662960 | 1.4:-70980061 | MS.gene07156:CDS |
| CACGAATTTGATGAAACTCG+CGG | 0.663191 | 1.4:-70980355 | MS.gene07156:CDS |
| ATAACAAACTCAGCGACGGT+GGG | 0.664181 | 1.4:+70980441 | None:intergenic |
| CAACAGACATGGTGTGCATG+AGG | 0.670248 | 1.4:+70980727 | None:intergenic |
| AAAATTCACATCTCCACCGG+CGG | 0.675896 | 1.4:+70980293 | None:intergenic |
| CTAACGCCGTTGATTCTCGA+CGG | 0.675903 | 1.4:-70979981 | MS.gene07156:CDS |
| TGAGATAACAAACTCAGCGA+CGG | 0.679630 | 1.4:+70980437 | None:intergenic |
| TATGTTGATGAAGAGAGTGG+TGG | 0.683276 | 1.4:+70980642 | None:intergenic |
| GTGTATGTTGATGAAGAGAG+TGG | 0.692828 | 1.4:+70980639 | None:intergenic |
| ACTGCATCACGGAAAACGTG+GGG | 0.694469 | 1.4:+70980486 | None:intergenic |
| CGACGGTTATAACTGCATCA+CGG | 0.697949 | 1.4:+70980475 | None:intergenic |
| TCAGCAGGTTCATACAAAGG+TGG | 0.707032 | 1.4:+70980591 | None:intergenic |
| ACGCCGTTGATTCTCGACGG+TGG | 0.707122 | 1.4:-70979978 | MS.gene07156:CDS |
| TTTACCGGTTGGTAACATGG+AGG | 0.712639 | 1.4:+70980551 | None:intergenic |
| GGTGGGACCTACCAAAACGA+CGG | 0.719809 | 1.4:+70980458 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATACGCAAGAGAAATAAA+CGG | - | chr1.4:70980377-70980396 | MS.gene07156:CDS | 25.0% |
| ! | GTCGAAAAATCATTTAAAAG+CGG | - | chr1.4:70980325-70980344 | MS.gene07156:CDS | 25.0% |
| ! | TGAATAGTAAAGATCAGAAT+GGG | - | chr1.4:70980540-70980559 | MS.gene07156:CDS | 25.0% |
| !! | ATTGAGTTTTTCGTTTCAAT+CGG | - | chr1.4:70980248-70980267 | MS.gene07156:CDS | 25.0% |
| !!! | AACAACATTTTTGGTTTTTC+TGG | + | chr1.4:70979494-70979513 | None:intergenic | 25.0% |
| !!! | GTGAATTTTAGAGATTCAAA+CGG | - | chr1.4:70979981-70980000 | MS.gene07156:CDS | 25.0% |
| GTGAATAGTAAAGATCAGAA+TGG | - | chr1.4:70980539-70980558 | MS.gene07156:CDS | 30.0% | |
| ! | TCTTTTCACGAAAGCTATAT+CGG | - | chr1.4:70979863-70979882 | MS.gene07156:CDS | 30.0% |
| ! | TGTTGATTGAAAAGGTTGTT+GGG | + | chr1.4:70979581-70979600 | None:intergenic | 30.0% |
| ! | TTGTTGATTGAAAAGGTTGT+TGG | + | chr1.4:70979582-70979601 | None:intergenic | 30.0% |
| !! | TAAGGGTTTGTTGATTGAAA+AGG | + | chr1.4:70979589-70979608 | None:intergenic | 30.0% |
| !! | TGCTTTTGAAAAGAAGCAAT+TGG | + | chr1.4:70980773-70980792 | None:intergenic | 30.0% |
| !!! | ACATTTTTGGTTTTTCTGGA+AGG | + | chr1.4:70979490-70979509 | None:intergenic | 30.0% |
| !!! | CATTTTTGGTTTTTCTGGAA+GGG | + | chr1.4:70979489-70979508 | None:intergenic | 30.0% |
| ATAGTAAAGATCAGAATGGG+TGG | - | chr1.4:70980543-70980562 | MS.gene07156:CDS | 35.0% | |
| ATTCCGTTTGCAATAAGAAC+AGG | - | chr1.4:70980014-70980033 | MS.gene07156:CDS | 35.0% | |
| CATACAAAGGTGGTTGATTA+AGG | + | chr1.4:70979662-70979681 | None:intergenic | 35.0% | |
| TACATTGGGCTTCATTCAAA+GGG | - | chr1.4:70980573-70980592 | MS.gene07156:CDS | 35.0% | |
| TGGACATTTACAAGTTGCAT+TGG | - | chr1.4:70980691-70980710 | MS.gene07156:CDS | 35.0% | |
| TTCCCTGTTCTTATTGCAAA+CGG | + | chr1.4:70980020-70980039 | None:intergenic | 35.0% | |
| TTCCGTTTGCAATAAGAACA+GGG | - | chr1.4:70980015-70980034 | MS.gene07156:CDS | 35.0% | |
| ! | AACAGCGTTTGAAATAGCAT+CGG | - | chr1.4:70980403-70980422 | MS.gene07156:CDS | 35.0% |
| ! | TCTAAAATTCACATCTCCAC+CGG | + | chr1.4:70979973-70979992 | None:intergenic | 35.0% |
| ! | TGAACGAGGAACAACATTTT+TGG | + | chr1.4:70979503-70979522 | None:intergenic | 35.0% |
| ! | TTCTCTTGCGTATTTTACGT+CGG | + | chr1.4:70980372-70980391 | None:intergenic | 35.0% |
| !! | CTTGTTTGGTGTACTACATT+GGG | - | chr1.4:70980442-70980461 | MS.gene07156:CDS | 35.0% |
| !! | TGTTGGGTTTGTTGTTGTTA+AGG | + | chr1.4:70979565-70979584 | None:intergenic | 35.0% |
| !! | TTGTTTGGTGTACTACATTG+GGG | - | chr1.4:70980443-70980462 | MS.gene07156:CDS | 35.0% |
| !!! | AAAGAGTGTCAAGGTTTTGT+TGG | - | chr1.4:70980601-70980620 | MS.gene07156:CDS | 35.0% |
| ATGCAGAATCAACGATTCAG+TGG | - | chr1.4:70980073-70980092 | MS.gene07156:CDS | 40.0% | |
| CACGAATTTGATGAAACTCG+CGG | - | chr1.4:70979905-70979924 | MS.gene07156:CDS | 40.0% | |
| CGACGAATTGGATGTGAATT+CGG | - | chr1.4:70980166-70980185 | MS.gene07156:CDS | 40.0% | |
| CTACATTGGGCTTCATTCAA+AGG | - | chr1.4:70980572-70980591 | MS.gene07156:CDS | 40.0% | |
| TAAGGAAACTGCAACAGACA+TGG | + | chr1.4:70979547-70979566 | None:intergenic | 40.0% | |
| TGAATCTTTCAGCGACGAAT+TGG | - | chr1.4:70980154-70980173 | MS.gene07156:CDS | 40.0% | |
| TGAATGAAGCCCAATGTAGT+GGG | + | chr1.4:70980571-70980590 | None:intergenic | 40.0% | |
| TGAGATAACAAACTCAGCGA+CGG | + | chr1.4:70979826-70979845 | None:intergenic | 40.0% | |
| TTGAATGAAGCCCAATGTAG+TGG | + | chr1.4:70980572-70980591 | None:intergenic | 40.0% | |
| ! | AGTGGTGGGATTACTGATAA+GGG | + | chr1.4:70979606-70979625 | None:intergenic | 40.0% |
| ! | ATGTTGATGAAGAGAGTGGT+GGG | + | chr1.4:70979620-70979639 | None:intergenic | 40.0% |
| ! | GAGTTTTTCGTTTCAATCGG+AGG | - | chr1.4:70980251-70980270 | MS.gene07156:CDS | 40.0% |
| ! | GTGTATGTTGATGAAGAGAG+TGG | + | chr1.4:70979624-70979643 | None:intergenic | 40.0% |
| ! | TATGTTGATGAAGAGAGTGG+TGG | + | chr1.4:70979621-70979640 | None:intergenic | 40.0% |
| ! | TGCAGTTATAACCGTCGTTT+TGG | - | chr1.4:70979791-70979810 | MS.gene07156:CDS | 40.0% |
| ! | TTTGAAATAGCATCGGAGAG+TGG | - | chr1.4:70980410-70980429 | MS.gene07156:CDS | 40.0% |
| !! | AGGGAGAATAAAGAGTGTCA+AGG | - | chr1.4:70980592-70980611 | MS.gene07156:CDS | 40.0% |
| !! | CAGGTTTGTTAAAGAGACGA+CGG | + | chr1.4:70979752-70979771 | None:intergenic | 40.0% |
| !! | GCTTGTTTGGTGTACTACAT+TGG | - | chr1.4:70980441-70980460 | MS.gene07156:CDS | 40.0% |
| !! | GTTATAACCGTCGTTTTGGT+AGG | - | chr1.4:70979795-70979814 | MS.gene07156:CDS | 40.0% |
| !! | GTTGATAGTGTTGATGATGC+TGG | - | chr1.4:70980638-70980657 | MS.gene07156:CDS | 40.0% |
| !! | TGAAGAAGTGTTTAGGGGAA+GGG | - | chr1.4:70980510-70980529 | MS.gene07156:CDS | 40.0% |
| !!! | GCATTGGTTTTGATTGCTCA+TGG | - | chr1.4:70980707-70980726 | MS.gene07156:CDS | 40.0% |
| !!! | GTCAAGGTTTTGTTGGAACA+TGG | - | chr1.4:70980608-70980627 | MS.gene07156:CDS | 40.0% |
| !!! | TTGGTTTTGATTGCTCATGG+TGG | - | chr1.4:70980710-70980729 | MS.gene07156:CDS | 40.0% |
| !!! | TTTGGTTTTTCTGGAAGGGA+AGG | + | chr1.4:70979485-70979504 | None:intergenic | 40.0% |
| AAAATTCACATCTCCACCGG+CGG | + | chr1.4:70979970-70979989 | None:intergenic | 45.0% | |
| AACTGCATCACGGAAAACGT+GGG | + | chr1.4:70979778-70979797 | None:intergenic | 45.0% | |
| AATGATGACACCAGTTCACG+TGG | - | chr1.4:70980199-70980218 | MS.gene07156:CDS | 45.0% | |
| ACGTCAGCAGGTTCATACAA+AGG | + | chr1.4:70979675-70979694 | None:intergenic | 45.0% | |
| ATAACAAACTCAGCGACGGT+GGG | + | chr1.4:70979822-70979841 | None:intergenic | 45.0% | |
| ATGAAACTCGCGGTTGAAAG+CGG | - | chr1.4:70979915-70979934 | MS.gene07156:CDS | 45.0% | |
| CGACGGTTATAACTGCATCA+CGG | + | chr1.4:70979788-70979807 | None:intergenic | 45.0% | |
| CGTCGTCTCTTTAACAAACC+TGG | - | chr1.4:70979750-70979769 | MS.gene07156:CDS | 45.0% | |
| CTGAAGCTTCTTGCAGCTAA+TGG | - | chr1.4:70980050-70980069 | MS.gene07156:CDS | 45.0% | |
| TAACTGCATCACGGAAAACG+TGG | + | chr1.4:70979779-70979798 | None:intergenic | 45.0% | |
| TCAGCAGGTTCATACAAAGG+TGG | + | chr1.4:70979672-70979691 | None:intergenic | 45.0% | |
| TGGTAACATGGAGGTTCGAA+CGG | + | chr1.4:70979703-70979722 | None:intergenic | 45.0% | |
| TTTGCACGAAGCTGCAACAA+TGG | - | chr1.4:70980103-70980122 | MS.gene07156:CDS | 45.0% | |
| ! | ATCGTCTGTAGTGGCTTTAC+CGG | + | chr1.4:70979727-70979746 | None:intergenic | 45.0% |
| ! | GAGTGGTGGGATTACTGATA+AGG | + | chr1.4:70979607-70979626 | None:intergenic | 45.0% |
| ! | TTTGTTGGAACATGGTGCTG+AGG | - | chr1.4:70980616-70980635 | MS.gene07156:CDS | 45.0% |
| !! | ACGCGCTGAAGAAGTGTTTA+GGG | - | chr1.4:70980504-70980523 | MS.gene07156:CDS | 45.0% |
| !! | CTGAAGAAGTGTTTAGGGGA+AGG | - | chr1.4:70980509-70980528 | MS.gene07156:CDS | 45.0% |
| !! | GAAGAAGTGTTTAGGGGAAG+GGG | - | chr1.4:70980511-70980530 | MS.gene07156:CDS | 45.0% |
| !! | TTTACCGGTTGGTAACATGG+AGG | + | chr1.4:70979712-70979731 | None:intergenic | 45.0% |
| ACTGCATCACGGAAAACGTG+GGG | + | chr1.4:70979777-70979796 | None:intergenic | 50.0% | |
| CAACAGACATGGTGTGCATG+AGG | + | chr1.4:70979536-70979555 | None:intergenic | 50.0% | |
| CGAACCTCCATGTTACCAAC+CGG | - | chr1.4:70979705-70979724 | MS.gene07156:CDS | 50.0% | |
| GAGGTTTGTGAGCTTGAACG+AGG | + | chr1.4:70979517-70979536 | None:intergenic | 50.0% | |
| GATAACAAACTCAGCGACGG+TGG | + | chr1.4:70979823-70979842 | None:intergenic | 50.0% | |
| GCTTCAGCAGCACAATGCAA+AGG | + | chr1.4:70980671-70980690 | None:intergenic | 50.0% | |
| TGAAACTCGCGGTTGAAAGC+GGG | - | chr1.4:70979916-70979935 | MS.gene07156:CDS | 50.0% | |
| TGTCCACCGTCGAGAATCAA+CGG | + | chr1.4:70980288-70980307 | None:intergenic | 50.0% | |
| TTCGACGCTGCATGATGAAG+TGG | + | chr1.4:70980311-70980330 | None:intergenic | 50.0% | |
| ! | TCTGTAGTGGCTTTACCGGT+TGG | + | chr1.4:70979723-70979742 | None:intergenic | 50.0% |
| ! | TTGCATTGTGCTGCTGAAGC+TGG | - | chr1.4:70980671-70980690 | MS.gene07156:CDS | 50.0% |
| !! | CACGCGCTGAAGAAGTGTTT+AGG | - | chr1.4:70980503-70980522 | MS.gene07156:CDS | 50.0% |
| !! | CGCGCTGAAGAAGTGTTTAG+GGG | - | chr1.4:70980505-70980524 | MS.gene07156:CDS | 50.0% |
| !! | CTAACGCCGTTGATTCTCGA+CGG | - | chr1.4:70980279-70980298 | MS.gene07156:CDS | 50.0% |
| !! | GGCTTTACCGGTTGGTAACA+TGG | + | chr1.4:70979715-70979734 | None:intergenic | 50.0% |
| ACGACGGAGATCGTCTGTAG+TGG | + | chr1.4:70979736-70979755 | None:intergenic | 55.0% | |
| AGTGGTCACACGCGCTTGTT+TGG | - | chr1.4:70980428-70980447 | MS.gene07156:CDS | 55.0% | |
| ATGGGTGGAGCCCACTACAT+TGG | - | chr1.4:70980558-70980577 | MS.gene07156:CDS | 55.0% | |
| GGTGGGACCTACCAAAACGA+CGG | + | chr1.4:70979805-70979824 | None:intergenic | 55.0% | |
| TGGGTGGAGCCCACTACATT+GGG | - | chr1.4:70980559-70980578 | MS.gene07156:CDS | 55.0% | |
| ! | GAAGTGTTTAGGGGAAGGGG+CGG | - | chr1.4:70980514-70980533 | MS.gene07156:CDS | 55.0% |
| GTCACCGATCTTATCGCCGC+CGG | - | chr1.4:70979951-70979970 | MS.gene07156:CDS | 60.0% | |
| GTTCACGTGGCAGCGAGTGA+AGG | - | chr1.4:70980212-70980231 | MS.gene07156:CDS | 60.0% | |
| TCACGGAAAACGTGGGGACC+AGG | + | chr1.4:70979771-70979790 | None:intergenic | 60.0% | |
| TCACTCGCTGCCACGTGAAC+TGG | + | chr1.4:70980212-70980231 | None:intergenic | 60.0% | |
| TCCACCGGCGGCGATAAGAT+CGG | + | chr1.4:70979958-70979977 | None:intergenic | 60.0% | |
| ! | CGAACGGTGATGACGTCAGC+AGG | + | chr1.4:70979687-70979706 | None:intergenic | 60.0% |
| !! | ACGCCGTTGATTCTCGACGG+TGG | - | chr1.4:70980282-70980301 | MS.gene07156:CDS | 60.0% |
| ACCGATCTTATCGCCGCCGG+TGG | - | chr1.4:70979954-70979973 | MS.gene07156:CDS | 65.0% | |
| GCTTCTACGAGCGGCGCGTG+TGG | - | chr1.4:70980472-70980491 | MS.gene07156:CDS | 70.0% | |
| GGGCGACGCGCTTCTACGAG+CGG | - | chr1.4:70980463-70980482 | MS.gene07156:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 70979444 | 70980838 | 70979444 | ID=MS.gene07156 |
| chr1.4 | mRNA | 70979444 | 70980838 | 70979444 | ID=MS.gene07156.t1;Parent=MS.gene07156 |
| chr1.4 | exon | 70979444 | 70980838 | 70979444 | ID=MS.gene07156.t1.exon1;Parent=MS.gene07156.t1 |
| chr1.4 | CDS | 70979444 | 70980838 | 70979444 | ID=cds.MS.gene07156.t1;Parent=MS.gene07156.t1 |
| Gene Sequence |
| Protein sequence |
