Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072609.t1 | XP_003626120.1 | 94.4 | 214 | 12 | 0 | 1 | 214 | 1 | 214 | 3.60E-105 | 391 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072609.t1 | Q9S8P4 | 84.8 | 191 | 29 | 0 | 24 | 214 | 26 | 216 | 7.7e-88 | 324.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072609.t1 | Q2HUY7 | 94.4 | 214 | 12 | 0 | 1 | 214 | 1 | 214 | 2.6e-105 | 391.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049265 | MS.gene072609 | 0.813831 | 2.10E-51 | -1.69E-46 |
| MS.gene049634 | MS.gene072609 | 0.812512 | 4.09E-51 | -1.69E-46 |
| MS.gene050083 | MS.gene072609 | 0.829604 | 4.69E-55 | -1.69E-46 |
| MS.gene050380 | MS.gene072609 | 0.827483 | 1.53E-54 | -1.69E-46 |
| MS.gene050381 | MS.gene072609 | 0.802719 | 4.91E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene072609 | -0.800358 | 1.50E-48 | -1.69E-46 |
| MS.gene050710 | MS.gene072609 | 0.803224 | 3.86E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene072609 | 0.985929 | 4.03E-165 | -1.69E-46 |
| MS.gene05080 | MS.gene072609 | -0.803458 | 3.45E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene072609 | -0.813711 | 2.23E-51 | -1.69E-46 |
| MS.gene05104 | MS.gene072609 | 0.820075 | 8.31E-53 | -1.69E-46 |
| MS.gene051632 | MS.gene072609 | 0.801643 | 8.17E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene072609 | 0.807635 | 4.59E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene072609 | 0.81346 | 2.53E-51 | -1.69E-46 |
| MS.gene052702 | MS.gene072609 | 0.821569 | 3.77E-53 | -1.69E-46 |
| MS.gene052805 | MS.gene072609 | 0.808336 | 3.26E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene072609 | -0.803939 | 2.74E-49 | -1.69E-46 |
| MS.gene053488 | MS.gene072609 | 0.80198 | 6.97E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene072609 | -0.801113 | 1.05E-48 | -1.69E-46 |
| MS.gene054290 | MS.gene072609 | 0.869242 | 3.59E-66 | -1.69E-46 |
| MS.gene054706 | MS.gene072609 | 0.810085 | 1.37E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene072609 | 0.815451 | 9.18E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene072609 | -0.816679 | 4.88E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene072609 | -0.807029 | 6.17E-50 | -1.69E-46 |
| MS.gene055959 | MS.gene072609 | 0.811971 | 5.36E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene072609 | 0.817321 | 3.50E-52 | -1.69E-46 |
| MS.gene056856 | MS.gene072609 | 0.826681 | 2.37E-54 | -1.69E-46 |
| MS.gene056857 | MS.gene072609 | 0.800776 | 1.23E-48 | -1.69E-46 |
| MS.gene056920 | MS.gene072609 | 0.801709 | 7.92E-49 | -1.69E-46 |
| MS.gene057544 | MS.gene072609 | 0.815462 | 9.13E-52 | -1.69E-46 |
| MS.gene058067 | MS.gene072609 | 0.845829 | 3.18E-59 | -1.69E-46 |
| MS.gene058093 | MS.gene072609 | 0.816008 | 6.90E-52 | -1.69E-46 |
| MS.gene058158 | MS.gene072609 | 0.834822 | 2.40E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene072609 | 0.832666 | 8.30E-56 | -1.69E-46 |
| MS.gene058603 | MS.gene072609 | 0.802993 | 4.31E-49 | -1.69E-46 |
| MS.gene058840 | MS.gene072609 | 0.840802 | 6.98E-58 | -1.69E-46 |
| MS.gene058928 | MS.gene072609 | -0.806682 | 7.31E-50 | -1.69E-46 |
| MS.gene059037 | MS.gene072609 | 0.813537 | 2.43E-51 | -1.69E-46 |
| MS.gene060205 | MS.gene072609 | 0.813367 | 2.65E-51 | -1.69E-46 |
| MS.gene060578 | MS.gene072609 | 0.809899 | 1.51E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene072609 | -0.820853 | 5.51E-53 | -1.69E-46 |
| MS.gene061041 | MS.gene072609 | -0.813335 | 2.70E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072609.t1 | MTR_7g111240 | 97.196 | 214 | 6 | 0 | 1 | 214 | 1 | 214 | 2.68e-150 | 416 |
| MS.gene072609.t1 | MTR_7g111240 | 95.794 | 214 | 6 | 1 | 1 | 214 | 1 | 211 | 2.98e-146 | 405 |
| MS.gene072609.t1 | MTR_1g085140 | 78.392 | 199 | 43 | 0 | 16 | 214 | 20 | 218 | 1.22e-112 | 321 |
| MS.gene072609.t1 | MTR_1g085500 | 65.700 | 207 | 71 | 0 | 8 | 214 | 11 | 217 | 1.61e-94 | 275 |
| MS.gene072609.t1 | MTR_6g005360 | 48.113 | 212 | 104 | 3 | 4 | 211 | 6 | 215 | 3.90e-56 | 177 |
| MS.gene072609.t1 | MTR_4g017040 | 46.047 | 215 | 109 | 3 | 1 | 210 | 3 | 215 | 6.08e-56 | 177 |
| MS.gene072609.t1 | MTR_6g005310 | 48.113 | 212 | 104 | 3 | 4 | 211 | 6 | 215 | 6.94e-56 | 177 |
| MS.gene072609.t1 | MTR_6g005330 | 48.792 | 207 | 100 | 3 | 9 | 211 | 11 | 215 | 1.00e-55 | 177 |
| MS.gene072609.t1 | MTR_1g079490 | 45.833 | 216 | 111 | 3 | 1 | 211 | 3 | 217 | 1.27e-55 | 176 |
| MS.gene072609.t1 | MTR_4g017050 | 45.581 | 215 | 110 | 3 | 1 | 210 | 3 | 215 | 2.64e-55 | 176 |
| MS.gene072609.t1 | MTR_5g046410 | 45.581 | 215 | 110 | 3 | 1 | 210 | 3 | 215 | 2.66e-55 | 176 |
| MS.gene072609.t1 | MTR_5g046430 | 45.581 | 215 | 110 | 3 | 1 | 210 | 3 | 215 | 2.66e-55 | 176 |
| MS.gene072609.t1 | MTR_4g017030 | 45.581 | 215 | 110 | 3 | 1 | 210 | 3 | 215 | 2.66e-55 | 176 |
| MS.gene072609.t1 | MTR_6g005340 | 47.642 | 212 | 105 | 3 | 4 | 211 | 6 | 215 | 4.08e-55 | 175 |
| MS.gene072609.t1 | MTR_2g019250 | 45.581 | 215 | 110 | 3 | 1 | 210 | 3 | 215 | 5.76e-55 | 175 |
| MS.gene072609.t1 | MTR_5g036570 | 45.641 | 195 | 105 | 1 | 19 | 212 | 26 | 220 | 1.44e-54 | 174 |
| MS.gene072609.t1 | MTR_1g112200 | 43.810 | 210 | 105 | 5 | 9 | 213 | 8 | 209 | 8.04e-53 | 169 |
| MS.gene072609.t1 | MTR_6g005380 | 50.829 | 181 | 82 | 3 | 24 | 199 | 26 | 204 | 9.23e-50 | 161 |
| MS.gene072609.t1 | MTR_1g023770 | 42.453 | 212 | 114 | 2 | 2 | 211 | 4 | 209 | 6.32e-49 | 159 |
| MS.gene072609.t1 | MTR_6g005350 | 43.961 | 207 | 109 | 4 | 9 | 211 | 11 | 214 | 6.53e-47 | 154 |
| MS.gene072609.t1 | MTR_2g030855 | 44.103 | 195 | 104 | 2 | 19 | 211 | 21 | 212 | 3.43e-46 | 152 |
| MS.gene072609.t1 | MTR_2g031270 | 43.158 | 190 | 103 | 2 | 24 | 211 | 27 | 213 | 6.66e-44 | 146 |
| MS.gene072609.t1 | MTR_1g060120 | 38.073 | 218 | 125 | 4 | 4 | 214 | 10 | 224 | 1.34e-43 | 146 |
| MS.gene072609.t1 | MTR_2g030895 | 43.605 | 172 | 94 | 2 | 43 | 211 | 7 | 178 | 3.36e-40 | 135 |
| MS.gene072609.t1 | MTR_2g030865 | 43.605 | 172 | 94 | 2 | 43 | 211 | 7 | 178 | 1.76e-39 | 134 |
| MS.gene072609.t1 | MTR_8g020630 | 37.559 | 213 | 128 | 4 | 2 | 214 | 1 | 208 | 2.89e-39 | 134 |
| MS.gene072609.t1 | MTR_8g007300 | 45.562 | 169 | 89 | 2 | 19 | 185 | 25 | 192 | 5.58e-39 | 134 |
| MS.gene072609.t1 | MTR_8g020650 | 38.542 | 192 | 114 | 3 | 23 | 214 | 21 | 208 | 1.68e-38 | 132 |
| MS.gene072609.t1 | MTR_8g020670 | 37.089 | 213 | 129 | 4 | 2 | 214 | 1 | 208 | 3.17e-38 | 131 |
| MS.gene072609.t1 | MTR_8g020590 | 37.438 | 203 | 125 | 1 | 9 | 211 | 4 | 204 | 3.95e-38 | 131 |
| MS.gene072609.t1 | MTR_3g096330 | 40.513 | 195 | 97 | 4 | 19 | 211 | 576 | 753 | 2.08e-37 | 138 |
| MS.gene072609.t1 | MTR_2g045100 | 37.441 | 211 | 127 | 4 | 2 | 212 | 1 | 206 | 2.79e-37 | 129 |
| MS.gene072609.t1 | MTR_2g041570 | 40.107 | 187 | 110 | 1 | 26 | 212 | 23 | 207 | 5.08e-37 | 128 |
| MS.gene072609.t1 | MTR_2g044040 | 36.967 | 211 | 128 | 4 | 2 | 212 | 1 | 206 | 8.20e-37 | 128 |
| MS.gene072609.t1 | MTR_2g041580 | 39.153 | 189 | 113 | 1 | 26 | 214 | 23 | 209 | 2.15e-36 | 127 |
| MS.gene072609.t1 | MTR_0284s0010 | 36.967 | 211 | 128 | 4 | 2 | 212 | 1 | 206 | 6.20e-36 | 125 |
| MS.gene072609.t1 | MTR_2g041550 | 40.323 | 186 | 109 | 1 | 26 | 211 | 23 | 206 | 6.94e-36 | 125 |
| MS.gene072609.t1 | MTR_3g019710 | 35.545 | 211 | 131 | 4 | 2 | 212 | 1 | 206 | 1.86e-35 | 124 |
| MS.gene072609.t1 | MTR_2g036120 | 36.019 | 211 | 130 | 4 | 2 | 212 | 1 | 206 | 3.28e-35 | 124 |
| MS.gene072609.t1 | MTR_3g019650 | 34.597 | 211 | 132 | 4 | 2 | 212 | 1 | 205 | 3.29e-34 | 121 |
| MS.gene072609.t1 | MTR_4g010340 | 40.196 | 204 | 114 | 5 | 8 | 211 | 6 | 201 | 4.29e-34 | 121 |
| MS.gene072609.t1 | MTR_7g011340 | 38.095 | 189 | 113 | 3 | 24 | 212 | 22 | 206 | 5.42e-34 | 120 |
| MS.gene072609.t1 | MTR_5g031460 | 32.701 | 211 | 137 | 4 | 2 | 212 | 1 | 206 | 2.14e-33 | 119 |
| MS.gene072609.t1 | MTR_3g019680 | 35.545 | 211 | 130 | 5 | 2 | 212 | 1 | 205 | 4.24e-33 | 118 |
| MS.gene072609.t1 | MTR_8g020770 | 39.560 | 182 | 105 | 4 | 2 | 182 | 1 | 178 | 3.56e-32 | 116 |
| MS.gene072609.t1 | MTR_2g086620 | 44.776 | 134 | 71 | 2 | 76 | 208 | 70 | 201 | 1.18e-31 | 114 |
| MS.gene072609.t1 | MTR_2g086630 | 44.776 | 134 | 71 | 2 | 76 | 208 | 70 | 201 | 1.44e-31 | 114 |
| MS.gene072609.t1 | MTR_8g020750 | 41.176 | 170 | 98 | 2 | 2 | 170 | 1 | 169 | 3.44e-31 | 114 |
| MS.gene072609.t1 | MTR_2g086640 | 36.842 | 209 | 115 | 6 | 1 | 208 | 8 | 200 | 4.20e-31 | 113 |
| MS.gene072609.t1 | MTR_8g020610 | 36.932 | 176 | 108 | 2 | 8 | 183 | 5 | 177 | 4.32e-31 | 113 |
| MS.gene072609.t1 | MTR_4g052770 | 41.045 | 134 | 76 | 2 | 76 | 208 | 71 | 202 | 3.48e-30 | 110 |
| MS.gene072609.t1 | MTR_4g052780 | 41.045 | 134 | 76 | 2 | 76 | 208 | 71 | 202 | 4.35e-30 | 110 |
| MS.gene072609.t1 | MTR_2g031300 | 40.881 | 159 | 72 | 2 | 43 | 199 | 7 | 145 | 9.61e-30 | 108 |
| MS.gene072609.t1 | MTR_8g020760 | 38.235 | 170 | 103 | 2 | 1 | 170 | 1 | 168 | 6.91e-26 | 100 |
| MS.gene072609.t1 | MTR_4g027415 | 44.715 | 123 | 66 | 2 | 24 | 146 | 22 | 142 | 1.93e-25 | 97.1 |
| MS.gene072609.t1 | MTR_8g020763 | 42.424 | 132 | 73 | 3 | 39 | 169 | 17 | 146 | 1.64e-24 | 95.9 |
| MS.gene072609.t1 | MTR_3g073170 | 34.574 | 188 | 115 | 5 | 25 | 211 | 26 | 206 | 3.19e-24 | 95.5 |
| MS.gene072609.t1 | MTR_2g019780 | 30.047 | 213 | 140 | 6 | 1 | 211 | 3 | 208 | 4.74e-22 | 90.1 |
| MS.gene072609.t1 | MTR_3g019810 | 33.014 | 209 | 125 | 7 | 2 | 208 | 107 | 302 | 1.21e-21 | 90.9 |
| MS.gene072609.t1 | MTR_2g072560 | 44.144 | 111 | 61 | 1 | 8 | 118 | 6 | 115 | 4.49e-21 | 85.5 |
| MS.gene072609.t1 | MTR_3g019610 | 42.991 | 107 | 59 | 2 | 2 | 108 | 1 | 105 | 4.50e-20 | 82.0 |
| MS.gene072609.t1 | MTR_2g030915 | 48.529 | 68 | 23 | 2 | 91 | 158 | 1 | 56 | 9.93e-14 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072609.t1 | AT1G09560 | 71.357 | 199 | 57 | 0 | 16 | 214 | 20 | 218 | 3.82e-98 | 284 |
| MS.gene072609.t1 | AT3G62020 | 67.633 | 207 | 65 | 2 | 10 | 214 | 12 | 218 | 7.31e-96 | 278 |
| MS.gene072609.t1 | AT1G02335 | 61.836 | 207 | 78 | 1 | 9 | 214 | 12 | 218 | 2.87e-89 | 262 |
| MS.gene072609.t1 | AT3G62020 | 67.956 | 181 | 57 | 1 | 35 | 214 | 9 | 189 | 5.57e-86 | 253 |
| MS.gene072609.t1 | AT5G26700 | 57.143 | 196 | 81 | 1 | 19 | 214 | 20 | 212 | 1.51e-76 | 229 |
| MS.gene072609.t1 | AT3G05930 | 53.140 | 207 | 96 | 1 | 9 | 214 | 12 | 218 | 1.88e-73 | 222 |
| MS.gene072609.t1 | AT1G18970 | 49.756 | 205 | 101 | 1 | 8 | 212 | 17 | 219 | 1.85e-63 | 196 |
| MS.gene072609.t1 | AT1G18980 | 50.254 | 197 | 96 | 1 | 16 | 212 | 25 | 219 | 3.26e-61 | 191 |
| MS.gene072609.t1 | AT5G39110 | 46.818 | 220 | 107 | 3 | 2 | 211 | 1 | 220 | 2.16e-60 | 189 |
| MS.gene072609.t1 | AT5G39150 | 47.489 | 219 | 106 | 3 | 2 | 211 | 1 | 219 | 2.24e-60 | 189 |
| MS.gene072609.t1 | AT3G05950 | 50.698 | 215 | 100 | 3 | 4 | 212 | 8 | 222 | 4.67e-60 | 188 |
| MS.gene072609.t1 | AT5G39120 | 47.032 | 219 | 107 | 3 | 2 | 211 | 1 | 219 | 1.25e-59 | 187 |
| MS.gene072609.t1 | AT5G39180 | 47.032 | 219 | 107 | 3 | 2 | 211 | 1 | 219 | 1.45e-59 | 186 |
| MS.gene072609.t1 | AT5G39160 | 46.411 | 209 | 108 | 2 | 8 | 212 | 12 | 220 | 4.21e-59 | 185 |
| MS.gene072609.t1 | AT5G39190 | 46.411 | 209 | 108 | 2 | 8 | 212 | 12 | 220 | 7.10e-59 | 185 |
| MS.gene072609.t1 | AT5G39130 | 46.411 | 209 | 108 | 2 | 8 | 212 | 12 | 220 | 1.32e-58 | 184 |
| MS.gene072609.t1 | AT4G14630 | 48.387 | 217 | 105 | 3 | 2 | 211 | 6 | 222 | 1.55e-58 | 184 |
| MS.gene072609.t1 | AT3G04200 | 47.768 | 224 | 106 | 4 | 1 | 213 | 1 | 224 | 6.12e-58 | 182 |
| MS.gene072609.t1 | AT5G39160 | 45.714 | 210 | 104 | 3 | 8 | 212 | 12 | 216 | 8.69e-56 | 177 |
| MS.gene072609.t1 | AT5G38960 | 47.805 | 205 | 102 | 3 | 13 | 212 | 19 | 223 | 2.16e-55 | 176 |
| MS.gene072609.t1 | AT5G38910 | 46.154 | 208 | 108 | 2 | 8 | 211 | 12 | 219 | 2.69e-55 | 176 |
| MS.gene072609.t1 | AT5G38940 | 47.208 | 197 | 100 | 2 | 19 | 211 | 25 | 221 | 3.76e-52 | 167 |
| MS.gene072609.t1 | AT5G38940 | 47.208 | 197 | 100 | 2 | 19 | 211 | 49 | 245 | 4.80e-52 | 168 |
| MS.gene072609.t1 | AT5G38930 | 46.500 | 200 | 103 | 2 | 16 | 211 | 22 | 221 | 5.93e-52 | 167 |
| MS.gene072609.t1 | AT5G39160 | 42.381 | 210 | 93 | 3 | 8 | 212 | 12 | 198 | 1.12e-49 | 160 |
| MS.gene072609.t1 | AT5G39190 | 42.381 | 210 | 93 | 3 | 8 | 212 | 12 | 198 | 1.36e-49 | 160 |
| MS.gene072609.t1 | AT3G04170 | 43.318 | 217 | 114 | 4 | 4 | 214 | 8 | 221 | 2.83e-49 | 160 |
| MS.gene072609.t1 | AT3G04150 | 42.791 | 215 | 114 | 4 | 4 | 209 | 8 | 222 | 1.38e-48 | 159 |
| MS.gene072609.t1 | AT3G04150 | 40.351 | 228 | 114 | 4 | 4 | 209 | 8 | 235 | 7.65e-48 | 157 |
| MS.gene072609.t1 | AT3G04190 | 43.814 | 194 | 105 | 2 | 20 | 209 | 26 | 219 | 1.58e-47 | 156 |
| MS.gene072609.t1 | AT3G04180 | 42.784 | 194 | 107 | 2 | 20 | 209 | 26 | 219 | 4.15e-47 | 155 |
| MS.gene072609.t1 | AT1G10460 | 41.000 | 200 | 113 | 3 | 17 | 211 | 16 | 215 | 4.29e-46 | 152 |
| MS.gene072609.t1 | AT5G61750 | 41.011 | 178 | 103 | 1 | 2 | 177 | 1 | 178 | 3.04e-43 | 144 |
| MS.gene072609.t1 | AT3G10080 | 37.931 | 203 | 118 | 4 | 16 | 213 | 22 | 221 | 2.90e-41 | 140 |
| MS.gene072609.t1 | AT3G10080 | 37.931 | 203 | 118 | 4 | 16 | 213 | 93 | 292 | 1.59e-40 | 140 |
| MS.gene072609.t1 | AT1G74820 | 39.894 | 188 | 111 | 1 | 24 | 211 | 40 | 225 | 1.66e-40 | 138 |
| MS.gene072609.t1 | AT5G20630 | 37.441 | 211 | 129 | 2 | 2 | 212 | 1 | 208 | 1.70e-38 | 132 |
| MS.gene072609.t1 | AT5G38910 | 37.864 | 206 | 91 | 2 | 8 | 211 | 12 | 182 | 4.41e-36 | 125 |
| MS.gene072609.t1 | AT1G72610 | 37.895 | 190 | 116 | 1 | 23 | 212 | 18 | 205 | 1.07e-35 | 125 |
| MS.gene072609.t1 | AT5G38950 | 42.857 | 63 | 36 | 0 | 140 | 202 | 2 | 64 | 4.50e-11 | 58.2 |
Find 59 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTTCCTTGGTACCAATT+TGG | 0.208001 | 7.4:-4934504 | None:intergenic |
| CCTCGCAGGGTTGTTGCTTC+TGG | 0.229387 | 7.4:+4933304 | MS.gene072609:CDS |
| GTACATAACCACTGTTCTTT+TGG | 0.233211 | 7.4:-4934369 | None:intergenic |
| TGGAAATGAACCAATCCTTT+AGG | 0.302524 | 7.4:-4934349 | None:intergenic |
| TTGACTCAAGCATTCCAAAT+TGG | 0.317621 | 7.4:+4934490 | MS.gene072609:CDS |
| GCTTGAGTCAAAATATTATC+TGG | 0.334327 | 7.4:-4934478 | None:intergenic |
| GATATCAAAGACTATTGTTA+AGG | 0.351789 | 7.4:+4934309 | MS.gene072609:CDS |
| TGTGTTGCAGACCTTGCTTC+TGG | 0.354120 | 7.4:+4933374 | MS.gene072609:CDS |
| CAATCCTTGATCTCACTACT+TGG | 0.371152 | 7.4:-4934551 | None:intergenic |
| GTTGTTTGTGGCTCCTGGTT+TGG | 0.387912 | 7.4:-4934092 | None:intergenic |
| GTACCTGCTGCAGTAATTGC+AGG | 0.389830 | 7.4:+4934388 | MS.gene072609:CDS |
| ATATCAAAGACTATTGTTAA+GGG | 0.409296 | 7.4:+4934310 | MS.gene072609:CDS |
| CAACACACTTGGTGTGTCAT+TGG | 0.413477 | 7.4:+4934165 | MS.gene072609:CDS |
| TTAACAGCGATTCTAGTGAA+TGG | 0.422863 | 7.4:+4934010 | MS.gene072609:intron |
| TTGAAGGTCAATTAGATGTT+GGG | 0.424187 | 7.4:+4934263 | MS.gene072609:CDS |
| AATGTGTTGTTTGTGGCTCC+TGG | 0.432005 | 7.4:-4934097 | None:intergenic |
| CACCAAGTGTGTTGAGTCCT+GGG | 0.432787 | 7.4:-4934156 | None:intergenic |
| GAAACATTTGTGTTTCCTAA+AGG | 0.432964 | 7.4:+4934334 | MS.gene072609:CDS |
| GTCAAAATATTATCTGGAAC+AGG | 0.434168 | 7.4:-4934472 | None:intergenic |
| GAGATTGTGTTTGTTCTTGA+AGG | 0.435097 | 7.4:+4934247 | MS.gene072609:CDS |
| GGAGCCACAAACAACACATT+TGG | 0.436644 | 7.4:+4934100 | MS.gene072609:CDS |
| CTTGAAGGTCAATTAGATGT+TGG | 0.439258 | 7.4:+4934262 | MS.gene072609:CDS |
| ATTTGTGTTTCCTAAAGGAT+TGG | 0.463106 | 7.4:+4934339 | MS.gene072609:CDS |
| AACATTGGCTAAGGTTACTA+AGG | 0.477034 | 7.4:-4934125 | None:intergenic |
| ACAGAGGTCTTGAAGATTGT+CGG | 0.481708 | 7.4:-4933354 | None:intergenic |
| CAGAGGTCTTGAAGATTGTC+GGG | 0.482659 | 7.4:-4933353 | None:intergenic |
| GTAGAAGCAAACAAAGTAAG+AGG | 0.485526 | 7.4:-4934445 | None:intergenic |
| TGGTTTGGCTAGTACGTTGG+TGG | 0.495482 | 7.4:-4934077 | None:intergenic |
| TCCTGGTTTGGCTAGTACGT+TGG | 0.504209 | 7.4:-4934080 | None:intergenic |
| GAAATCAGCTGCGGTTACAT+TGG | 0.516657 | 7.4:-4934053 | None:intergenic |
| AATCCTGCAATTACTGCAGC+AGG | 0.520151 | 7.4:-4934391 | None:intergenic |
| GCTGCGGTTACATTGGAAGC+TGG | 0.520487 | 7.4:-4934046 | None:intergenic |
| ACACCAAGTGTGTTGAGTCC+TGG | 0.524633 | 7.4:-4934157 | None:intergenic |
| GGAACCAAATGTGTTGTTTG+TGG | 0.527740 | 7.4:-4934104 | None:intergenic |
| CAAATGATGAAGCTCCTCGC+AGG | 0.535137 | 7.4:+4933290 | None:intergenic |
| GCCAATGTTCAGAAAATCCC+AGG | 0.563692 | 7.4:+4934139 | MS.gene072609:CDS |
| ATCCCAGGACTCAACACACT+TGG | 0.570656 | 7.4:+4934154 | MS.gene072609:CDS |
| CACGTGGGTGAGTGTGAGGT+GGG | 0.576033 | 7.4:-4934219 | None:intergenic |
| AAGTTCAGAAAATCAAGTCT+AGG | 0.576600 | 7.4:+4934521 | MS.gene072609:CDS |
| GCACGTGGGTGAGTGTGAGG+TGG | 0.584372 | 7.4:-4934220 | None:intergenic |
| AAATGATGAAGCTCCTCGCA+GGG | 0.586522 | 7.4:+4933291 | None:intergenic |
| AGCATTCCAAATTGGTACCA+AGG | 0.587841 | 7.4:+4934498 | MS.gene072609:CDS |
| AATCCTTGATCTCACTACTT+GGG | 0.605443 | 7.4:-4934550 | None:intergenic |
| CAATAGTCAATTGCAAGGAA+CGG | 0.606871 | 7.4:+4934414 | MS.gene072609:CDS |
| AACACAATCTCAGTTGCACG+TGG | 0.610170 | 7.4:-4934235 | None:intergenic |
| ACCAACGTACTAGCCAAACC+AGG | 0.618402 | 7.4:+4934079 | MS.gene072609:CDS |
| CGTATTGACTATGCTGTAGG+TGG | 0.621828 | 7.4:+4934190 | MS.gene072609:CDS |
| GTTCATTTCCAAAAGAACAG+TGG | 0.623665 | 7.4:+4934361 | MS.gene072609:CDS |
| AAAATATTATCTGGAACAGG+TGG | 0.624953 | 7.4:-4934469 | None:intergenic |
| ATGTAACGGTACCAGAAGCA+AGG | 0.638804 | 7.4:-4933385 | None:intergenic |
| GGATTCAATAGTCAATTGCA+AGG | 0.643876 | 7.4:+4934409 | MS.gene072609:CDS |
| GCACGTATTGACTATGCTGT+AGG | 0.654744 | 7.4:+4934187 | MS.gene072609:CDS |
| GTTGCACGTGGGTGAGTGTG+AGG | 0.660628 | 7.4:-4934223 | None:intergenic |
| GTCGGGATCAGCAGTGACAG+AGG | 0.662254 | 7.4:-4933336 | None:intergenic |
| CCAGAAGCAACAACCCTGCG+AGG | 0.671018 | 7.4:-4933304 | None:intergenic |
| GGGATCAGCAGTGACAGAGG+TGG | 0.687303 | 7.4:-4933333 | None:intergenic |
| GTTGGTGGAGAAATCAGCTG+CGG | 0.730459 | 7.4:-4934062 | None:intergenic |
| ACACAATCTCAGTTGCACGT+GGG | 0.741428 | 7.4:-4934234 | None:intergenic |
| AAGCAAGGTCTGCAACACAG+AGG | 0.751663 | 7.4:-4933370 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAATATAAGAAAG+AGG | - | chr7.4:4933592-4933611 | None:intergenic | 10.0% |
| !! | AAAGTTAATCATATTCATAT+AGG | - | chr7.4:4933718-4933737 | None:intergenic | 15.0% |
| !! | GATACTATTATAAAAGATAA+GGG | - | chr7.4:4933752-4933771 | None:intergenic | 15.0% |
| !! | TGATACTATTATAAAAGATA+AGG | - | chr7.4:4933753-4933772 | None:intergenic | 15.0% |
| !! | ATATCAAAGACTATTGTTAA+GGG | + | chr7.4:4934310-4934329 | MS.gene072609:CDS | 20.0% |
| !! | TTAATCATATTCATATAGGT+GGG | - | chr7.4:4933714-4933733 | None:intergenic | 20.0% |
| ! | AGGATTGCATATAATGATTA+AGG | - | chr7.4:4933572-4933591 | None:intergenic | 25.0% |
| ! | GATATCAAAGACTATTGTTA+AGG | + | chr7.4:4934309-4934328 | MS.gene072609:CDS | 25.0% |
| ! | GGAAAAAGTGAAAATGAAAA+GGG | - | chr7.4:4933550-4933569 | None:intergenic | 25.0% |
| ! | GGATTGCATATAATGATTAA+GGG | - | chr7.4:4933571-4933590 | None:intergenic | 25.0% |
| ! | GTACTTAGCAACATTAATTA+TGG | + | chr7.4:4933499-4933518 | MS.gene072609:intron | 25.0% |
| ! | GTTAATCATATTCATATAGG+TGG | - | chr7.4:4933715-4933734 | None:intergenic | 25.0% |
| ! | TCAATAAAAAGCCATATCAA+AGG | - | chr7.4:4933661-4933680 | None:intergenic | 25.0% |
| ! | TTATGGTTGAATTATTGTGA+TGG | + | chr7.4:4933959-4933978 | MS.gene072609:intron | 25.0% |
| ! | TTCTTGTCTTACAATTCTTA+TGG | + | chr7.4:4933942-4933961 | MS.gene072609:intron | 25.0% |
| !! | CATATTATTGATAGCATGAT+TGG | + | chr7.4:4933844-4933863 | MS.gene072609:intron | 25.0% |
| AAGTTCAGAAAATCAAGTCT+AGG | + | chr7.4:4934521-4934540 | MS.gene072609:CDS | 30.0% | |
| ATCCAACTAACTCTCTAATT+AGG | + | chr7.4:4933625-4933644 | MS.gene072609:intron | 30.0% | |
| ATTTGTGTTTCCTAAAGGAT+TGG | + | chr7.4:4934339-4934358 | MS.gene072609:CDS | 30.0% | |
| CAATTATAGTGTCTTTCTGA+AGG | + | chr7.4:4933779-4933798 | MS.gene072609:intron | 30.0% | |
| CTTCAGAAAGACACTATAAT+TGG | - | chr7.4:4933781-4933800 | None:intergenic | 30.0% | |
| GAAACATTTGTGTTTCCTAA+AGG | + | chr7.4:4934334-4934353 | MS.gene072609:CDS | 30.0% | |
| GCTTGAGTCAAAATATTATC+TGG | - | chr7.4:4934481-4934500 | None:intergenic | 30.0% | |
| GGGAAAAAGTGAAAATGAAA+AGG | - | chr7.4:4933551-4933570 | None:intergenic | 30.0% | |
| TTAATTATGGATCGAAAAGC+TGG | + | chr7.4:4933512-4933531 | MS.gene072609:intron | 30.0% | |
| TTGAAGGTCAATTAGATGTT+GGG | + | chr7.4:4934263-4934282 | MS.gene072609:CDS | 30.0% | |
| ! | AAAATATTATCTGGAACAGG+TGG | - | chr7.4:4934472-4934491 | None:intergenic | 30.0% |
| ! | CGATATACATACTTGTTTCT+TGG | + | chr7.4:4933910-4933929 | MS.gene072609:intron | 30.0% |
| ! | GTCAAAATATTATCTGGAAC+AGG | - | chr7.4:4934475-4934494 | None:intergenic | 30.0% |
| CAATAGTCAATTGCAAGGAA+CGG | + | chr7.4:4934414-4934433 | MS.gene072609:CDS | 35.0% | |
| CTTGAAGGTCAATTAGATGT+TGG | + | chr7.4:4934262-4934281 | MS.gene072609:CDS | 35.0% | |
| GAGATTGTGTTTGTTCTTGA+AGG | + | chr7.4:4934247-4934266 | MS.gene072609:CDS | 35.0% | |
| GGATTCAATAGTCAATTGCA+AGG | + | chr7.4:4934409-4934428 | MS.gene072609:CDS | 35.0% | |
| GTAGAAGCAAACAAAGTAAG+AGG | - | chr7.4:4934448-4934467 | None:intergenic | 35.0% | |
| GTTCATTTCCAAAAGAACAG+TGG | + | chr7.4:4934361-4934380 | MS.gene072609:CDS | 35.0% | |
| TGGAAATGAACCAATCCTTT+AGG | - | chr7.4:4934352-4934371 | None:intergenic | 35.0% | |
| TGGTTGAATTATTGTGATGG+TGG | + | chr7.4:4933962-4933981 | MS.gene072609:intron | 35.0% | |
| TTAACAGCGATTCTAGTGAA+TGG | + | chr7.4:4934010-4934029 | MS.gene072609:intron | 35.0% | |
| TTGACTCAAGCATTCCAAAT+TGG | + | chr7.4:4934490-4934509 | MS.gene072609:CDS | 35.0% | |
| ! | AAAAGCCATATCAAAGGTGT+TGG | - | chr7.4:4933655-4933674 | None:intergenic | 35.0% |
| ! | AACATTGGCTAAGGTTACTA+AGG | - | chr7.4:4934128-4934147 | None:intergenic | 35.0% |
| ! | CTTGATTTTCTGAACTTCCT+TGG | - | chr7.4:4934518-4934537 | None:intergenic | 35.0% |
| ! | GTACATAACCACTGTTCTTT+TGG | - | chr7.4:4934372-4934391 | None:intergenic | 35.0% |
| !! | GATTTTCTGAACATTGGCTA+AGG | - | chr7.4:4934137-4934156 | None:intergenic | 35.0% |
| ACAGAGGTCTTGAAGATTGT+CGG | - | chr7.4:4933357-4933376 | None:intergenic | 40.0% | |
| AGCATTCCAAATTGGTACCA+AGG | + | chr7.4:4934498-4934517 | MS.gene072609:CDS | 40.0% | |
| GAACTTCCTTGGTACCAATT+TGG | - | chr7.4:4934507-4934526 | None:intergenic | 40.0% | |
| GGCCTAATTAGAGAGTTAGT+TGG | - | chr7.4:4933630-4933649 | None:intergenic | 40.0% | |
| ! | TCCTGGGATTTTCTGAACAT+TGG | - | chr7.4:4934143-4934162 | None:intergenic | 40.0% |
| !! | GGAACCAAATGTGTTGTTTG+TGG | - | chr7.4:4934107-4934126 | None:intergenic | 40.0% |
| AACACAATCTCAGTTGCACG+TGG | - | chr7.4:4934238-4934257 | None:intergenic | 45.0% | |
| AATCCTGCAATTACTGCAGC+AGG | - | chr7.4:4934394-4934413 | None:intergenic | 45.0% | |
| ACACAATCTCAGTTGCACGT+GGG | - | chr7.4:4934237-4934256 | None:intergenic | 45.0% | |
| ATGTAACGGTACCAGAAGCA+AGG | - | chr7.4:4933388-4933407 | None:intergenic | 45.0% | |
| CAGAGGTCTTGAAGATTGTC+GGG | - | chr7.4:4933356-4933375 | None:intergenic | 45.0% | |
| GAAATCAGCTGCGGTTACAT+TGG | - | chr7.4:4934056-4934075 | None:intergenic | 45.0% | |
| GCCAATGTTCAGAAAATCCC+AGG | + | chr7.4:4934139-4934158 | MS.gene072609:CDS | 45.0% | |
| GCCTACCAACACCTTTGATA+TGG | + | chr7.4:4933647-4933666 | MS.gene072609:intron | 45.0% | |
| GGAGCCACAAACAACACATT+TGG | + | chr7.4:4934100-4934119 | MS.gene072609:CDS | 45.0% | |
| !! | AATGTGTTGTTTGTGGCTCC+TGG | - | chr7.4:4934100-4934119 | None:intergenic | 45.0% |
| !! | CAACACACTTGGTGTGTCAT+TGG | + | chr7.4:4934165-4934184 | MS.gene072609:CDS | 45.0% |
| !! | CGTATTGACTATGCTGTAGG+TGG | + | chr7.4:4934190-4934209 | MS.gene072609:CDS | 45.0% |
| !! | GCACGTATTGACTATGCTGT+AGG | + | chr7.4:4934187-4934206 | MS.gene072609:CDS | 45.0% |
| !! | GCCATATCAAAGGTGTTGGT+AGG | - | chr7.4:4933651-4933670 | None:intergenic | 45.0% |
| !! | ATTATTATTAATTAATGTAA+CGG | - | chr7.4:4933402-4933421 | None:intergenic | 5.0% |
| AAGCAAGGTCTGCAACACAG+AGG | - | chr7.4:4933373-4933392 | None:intergenic | 50.0% | |
| ACACCAAGTGTGTTGAGTCC+TGG | - | chr7.4:4934160-4934179 | None:intergenic | 50.0% | |
| ACCAACGTACTAGCCAAACC+AGG | + | chr7.4:4934079-4934098 | MS.gene072609:CDS | 50.0% | |
| ATCCCAGGACTCAACACACT+TGG | + | chr7.4:4934154-4934173 | MS.gene072609:CDS | 50.0% | |
| CACCAAGTGTGTTGAGTCCT+GGG | - | chr7.4:4934159-4934178 | None:intergenic | 50.0% | |
| GTACCTGCTGCAGTAATTGC+AGG | + | chr7.4:4934388-4934407 | MS.gene072609:CDS | 50.0% | |
| GTTGTTTGTGGCTCCTGGTT+TGG | - | chr7.4:4934095-4934114 | None:intergenic | 50.0% | |
| TGTGTTGCAGACCTTGCTTC+TGG | + | chr7.4:4933374-4933393 | MS.gene072609:CDS | 50.0% | |
| ! | GTTGGTGGAGAAATCAGCTG+CGG | - | chr7.4:4934065-4934084 | None:intergenic | 50.0% |
| ! | TGGTTTGGCTAGTACGTTGG+TGG | - | chr7.4:4934080-4934099 | None:intergenic | 50.0% |
| !! | TCCTGGTTTGGCTAGTACGT+TGG | - | chr7.4:4934083-4934102 | None:intergenic | 50.0% |
| GCTGCGGTTACATTGGAAGC+TGG | - | chr7.4:4934049-4934068 | None:intergenic | 55.0% | |
| CACGTGGGTGAGTGTGAGGT+GGG | - | chr7.4:4934222-4934241 | None:intergenic | 60.0% | |
| CCAGAAGCAACAACCCTGCG+AGG | - | chr7.4:4933307-4933326 | None:intergenic | 60.0% | |
| GGGATCAGCAGTGACAGAGG+TGG | - | chr7.4:4933336-4933355 | None:intergenic | 60.0% | |
| GTCGGGATCAGCAGTGACAG+AGG | - | chr7.4:4933339-4933358 | None:intergenic | 60.0% | |
| GTTGCACGTGGGTGAGTGTG+AGG | - | chr7.4:4934226-4934245 | None:intergenic | 60.0% | |
| ! | CCTCGCAGGGTTGTTGCTTC+TGG | + | chr7.4:4933304-4933323 | MS.gene072609:CDS | 60.0% |
| GCACGTGGGTGAGTGTGAGG+TGG | - | chr7.4:4934223-4934242 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 4933293 | 4934558 | 4933293 | ID=MS.gene072609 |
| chr7.4 | mRNA | 4933293 | 4934558 | 4933293 | ID=MS.gene072609.t1;Parent=MS.gene072609 |
| chr7.4 | exon | 4933293 | 4933395 | 4933293 | ID=MS.gene072609.t1.exon1;Parent=MS.gene072609.t1 |
| chr7.4 | CDS | 4933293 | 4933395 | 4933293 | ID=cds.MS.gene072609.t1;Parent=MS.gene072609.t1 |
| chr7.4 | exon | 4934017 | 4934558 | 4934017 | ID=MS.gene072609.t1.exon2;Parent=MS.gene072609.t1 |
| chr7.4 | CDS | 4934017 | 4934558 | 4934017 | ID=cds.MS.gene072609.t1;Parent=MS.gene072609.t1 |
| Gene Sequence |
| Protein sequence |
