AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073286


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073286.t1 MTR_4g127870 95.930 172 7 0 1 172 1 172 3.80e-126 352
MS.gene073286.t1 MTR_6g018310 49.405 168 81 3 3 169 15 179 1.02e-60 186
MS.gene073286.t1 MTR_6g018300 50.610 164 77 3 7 169 16 176 1.03e-60 186
MS.gene073286.t1 MTR_7g007120 50.641 156 73 3 15 169 28 180 6.68e-58 179
MS.gene073286.t1 MTR_7g007120 51.299 154 71 3 15 167 28 178 9.89e-58 179
MS.gene073286.t1 MTR_7g007120 51.299 154 71 3 15 167 28 178 1.12e-57 179
MS.gene073286.t1 MTR_7g007220 51.299 154 71 3 15 167 28 178 1.13e-57 179
MS.gene073286.t1 MTR_7g007200 51.299 154 71 3 15 167 28 178 1.51e-57 178
MS.gene073286.t1 MTR_7g007230 50.649 154 72 3 15 167 28 178 1.29e-56 176
MS.gene073286.t1 MTR_7g007210 52.143 140 63 3 15 153 28 164 9.86e-52 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073286.t1 AT1G67090 49.432 176 82 3 1 171 6 179 6.57e-62 189
MS.gene073286.t1 AT5G38420 52.121 165 75 3 8 171 18 179 4.16e-61 187
MS.gene073286.t1 AT5G38410 51.515 165 76 3 8 171 18 179 4.35e-61 187
MS.gene073286.t1 AT5G38430 51.515 165 76 3 8 171 18 179 1.48e-60 186
MS.gene073286.t1 AT5G38430 51.515 165 76 3 8 171 18 179 1.48e-60 186
MS.gene073286.t1 AT5G38410 49.412 170 77 3 8 171 18 184 1.54e-59 184
MS.gene073286.t1 AT5G38410 50.610 164 72 4 8 171 18 172 1.95e-57 178
MS.gene073286.t1 AT1G67090 39.130 115 64 2 1 111 6 118 3.25e-22 87.0

Find 44 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATTTCCTATTATTAA+TGG 0.204312 8.1:-4932258 None:intergenic
GCAGCTTCAGTTGCTGGGCC+TGG 0.282618 8.1:+4931975 MS.gene073286:CDS
ATCAGAAAGGGGTGGTAAAT+AGG 0.291264 8.1:-4932288 None:intergenic
ATGGGAGGTATTGGACATTA+TGG 0.311655 8.1:+4932944 MS.gene073286:CDS
TCAAATTTCCTATTATTAAT+GGG 0.315443 8.1:-4932257 None:intergenic
CATGGAATCCCATTAATAAT+AGG 0.318513 8.1:+4932249 MS.gene073286:CDS
GTTGCTGGGCCTGGCCTTGT+GGG 0.329334 8.1:+4931984 MS.gene073286:CDS
AGTCCAACTCATCAAATCTT+TGG 0.338452 8.1:+4932012 MS.gene073286:CDS
ACATTTGCAGCTTCAGTTGC+TGG 0.348801 8.1:+4931969 MS.gene073286:CDS
AAGCATCTTATATATGCATT+TGG 0.378298 8.1:-4933039 None:intergenic
TAAGTAGTTGTTGTGGTAGT+AGG 0.382906 8.1:-4933114 None:intergenic
TTCAATGGAATAAGTAGTAC+TGG 0.385537 8.1:-4932036 None:intergenic
CATTTGCAGCTTCAGTTGCT+GGG 0.394890 8.1:+4931970 MS.gene073286:CDS
GATACTATGATGGGAGGTAT+TGG 0.398812 8.1:+4932935 MS.gene073286:CDS
GTACTACTTATTCCATTGAA+TGG 0.406489 8.1:+4932039 MS.gene073286:CDS
GACTTCAAGCCCACAAGGCC+AGG 0.408478 8.1:-4931993 None:intergenic
TTATGGAAGCTACCAATGTT+TGG 0.409185 8.1:+4932961 MS.gene073286:CDS
AGTTGCTGGGCCTGGCCTTG+TGG 0.426737 8.1:+4931983 MS.gene073286:CDS
TGCAATTGAGTCATCAGAAA+GGG 0.441885 8.1:-4932300 None:intergenic
GACTAACCTCATCAAATTCA+AGG 0.447817 8.1:-4932364 None:intergenic
CATCATAGTATCCTGGCATC+TGG 0.482846 8.1:-4932924 None:intergenic
GCAGTGAATCTTAGAACCAT+TGG 0.489711 8.1:-4932078 None:intergenic
CAGATGCCAGGATACTATGA+TGG 0.514747 8.1:+4932925 MS.gene073286:CDS
GGGCCAAAGATTTGATGAGT+TGG 0.537281 8.1:-4932015 None:intergenic
GATTGACTACATGCTCAAGA+AGG 0.539499 8.1:+4932328 MS.gene073286:CDS
TACCTCCCATCATAGTATCC+TGG 0.541106 8.1:-4932931 None:intergenic
TTCTGATGACTCAATTGCAA+AGG 0.545642 8.1:+4932304 MS.gene073286:CDS
TTTGTGAATAATAAAAGCCA+TGG 0.556871 8.1:-4933091 None:intergenic
TTCTAAGATTCACTGCATGA+AGG 0.557279 8.1:+4932085 MS.gene073286:CDS
TTGCAATTGAGTCATCAGAA+AGG 0.572127 8.1:-4932301 None:intergenic
AGAGCAAATAGCCAGATGCC+AGG 0.574689 8.1:+4932913 MS.gene073286:CDS
ACCTCATCAAATTCAAGGCA+AGG 0.584505 8.1:-4932359 None:intergenic
AGATGCCAGGATACTATGAT+GGG 0.593649 8.1:+4932926 MS.gene073286:CDS
CAAGCGACACATGCAAGCCA+TGG 0.600771 8.1:+4933074 MS.gene073286:CDS
ATTGACTACATGCTCAAGAA+GGG 0.603348 8.1:+4932329 MS.gene073286:CDS
ACCTTGCCTTGAATTTGATG+AGG 0.604745 8.1:+4932358 MS.gene073286:CDS
ACTACATGCTCAAGAAGGGA+TGG 0.608538 8.1:+4932333 MS.gene073286:CDS
AGTTGGACTTCAAGCCCACA+AGG 0.611785 8.1:-4931998 None:intergenic
AATTAATTAAGTAGTTGTTG+TGG 0.614403 8.1:-4933121 None:intergenic
GCAATTGAGTCATCAGAAAG+GGG 0.629169 8.1:-4932299 None:intergenic
TCAATGGAATAAGTAGTACT+GGG 0.635347 8.1:-4932035 None:intergenic
TGCCAGGATACTATGATGGG+AGG 0.662604 8.1:+4932929 MS.gene073286:CDS
GAGTCGTTACAACCAAACAT+TGG 0.684048 8.1:-4932973 None:intergenic
ATTGAGTCATCAGAAAGGGG+TGG 0.697277 8.1:-4932296 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATATCTTATTAATAATA+TGG + chr8.1:4932850-4932869 MS.gene073286:intron 10.0%
!! TTATATTATAAACTAGATTT+AGG - chr8.1:4932393-4932412 None:intergenic 10.0%
!!! TAAATCTAGTTTATAATATA+AGG + chr8.1:4932392-4932411 MS.gene073286:intron 10.0%
!! GATTCATATAATATAGATTA+AGG + chr8.1:4932482-4932501 MS.gene073286:intron 15.0%
!! TCAAATTTCCTATTATTAAT+GGG - chr8.1:4932260-4932279 None:intergenic 15.0%
!! TTCAAATTTCCTATTATTAA+TGG - chr8.1:4932261-4932280 None:intergenic 15.0%
!!! AAGTTATACAAAAGTTTTAT+TGG - chr8.1:4932768-4932787 None:intergenic 15.0%
!! ATTATAAACTAGATTTAGGT+TGG - chr8.1:4932389-4932408 None:intergenic 20.0%
!! ATTATAAGGAAAAAACAACT+AGG + chr8.1:4932429-4932448 MS.gene073286:intron 20.0%
!! TAGAAAACAAAAACATCAAA+AGG - chr8.1:4932825-4932844 None:intergenic 20.0%
!!! CTTTAATTTATTCTATGAAG+TGG + chr8.1:4932785-4932804 MS.gene073286:intron 20.0%
! AAAAAGAAAAAGACCAAGAT+GGG - chr8.1:4932174-4932193 None:intergenic 25.0%
! AACCATGCTATCATAATATA+TGG - chr8.1:4932132-4932151 None:intergenic 25.0%
! AAGCATCTTATATATGCATT+TGG - chr8.1:4933042-4933061 None:intergenic 25.0%
! AATATGGAACTTGTATTGTT+TGG + chr8.1:4932866-4932885 MS.gene073286:intron 25.0%
! ATATAGATTAAGGCAATGTT+TGG + chr8.1:4932492-4932511 MS.gene073286:intron 25.0%
! TTTGTAACTTAAAACAGACA+TGG + chr8.1:4932231-4932250 MS.gene073286:intron 25.0%
! TTTGTGAATAATAAAAGCCA+TGG - chr8.1:4933094-4933113 None:intergenic 25.0%
AGCCATATATTATGATAGCA+TGG + chr8.1:4932127-4932146 MS.gene073286:intron 30.0%
ATTGTTTGGAAATGGAAGTT+TGG + chr8.1:4932880-4932899 MS.gene073286:intron 30.0%
CATGGAATCCCATTAATAAT+AGG + chr8.1:4932249-4932268 MS.gene073286:CDS 30.0%
GAAAAAGAAAAAGACCAAGA+TGG - chr8.1:4932175-4932194 None:intergenic 30.0%
GAACTTGTATTGTTTGGAAA+TGG + chr8.1:4932872-4932891 MS.gene073286:intron 30.0%
GTACTACTTATTCCATTGAA+TGG + chr8.1:4932039-4932058 MS.gene073286:CDS 30.0%
TCAATGGAATAAGTAGTACT+GGG - chr8.1:4932038-4932057 None:intergenic 30.0%
TTCAATGGAATAAGTAGTAC+TGG - chr8.1:4932039-4932058 None:intergenic 30.0%
! AGTCTTCTTTTTCCATTCAA+TGG - chr8.1:4932054-4932073 None:intergenic 30.0%
AAAAAGAAGACTGTCTCCAA+TGG + chr8.1:4932062-4932081 MS.gene073286:CDS 35.0%
AGTCCAACTCATCAAATCTT+TGG + chr8.1:4932012-4932031 MS.gene073286:CDS 35.0%
ATTGACTACATGCTCAAGAA+GGG + chr8.1:4932329-4932348 MS.gene073286:CDS 35.0%
GACTAACCTCATCAAATTCA+AGG - chr8.1:4932367-4932386 None:intergenic 35.0%
TAAGTAGTTGTTGTGGTAGT+AGG - chr8.1:4933117-4933136 None:intergenic 35.0%
TGCAATTGAGTCATCAGAAA+GGG - chr8.1:4932303-4932322 None:intergenic 35.0%
TTATGGAAGCTACCAATGTT+TGG + chr8.1:4932961-4932980 MS.gene073286:CDS 35.0%
TTCTGATGACTCAATTGCAA+AGG + chr8.1:4932304-4932323 MS.gene073286:CDS 35.0%
TTGCAATTGAGTCATCAGAA+AGG - chr8.1:4932304-4932323 None:intergenic 35.0%
!! TTCTAAGATTCACTGCATGA+AGG + chr8.1:4932085-4932104 MS.gene073286:CDS 35.0%
ACCTCATCAAATTCAAGGCA+AGG - chr8.1:4932362-4932381 None:intergenic 40.0%
ACCTTGCCTTGAATTTGATG+AGG + chr8.1:4932358-4932377 MS.gene073286:CDS 40.0%
AGATGCCAGGATACTATGAT+GGG + chr8.1:4932926-4932945 MS.gene073286:CDS 40.0%
ATCAGAAAGGGGTGGTAAAT+AGG - chr8.1:4932291-4932310 None:intergenic 40.0%
ATGTACTAGTACTCCCATCT+TGG + chr8.1:4932158-4932177 MS.gene073286:intron 40.0%
GAGTCGTTACAACCAAACAT+TGG - chr8.1:4932976-4932995 None:intergenic 40.0%
GATTGACTACATGCTCAAGA+AGG + chr8.1:4932328-4932347 MS.gene073286:CDS 40.0%
GCAATTGAGTCATCAGAAAG+GGG - chr8.1:4932302-4932321 None:intergenic 40.0%
GCAGTGAATCTTAGAACCAT+TGG - chr8.1:4932081-4932100 None:intergenic 40.0%
! GATACTATGATGGGAGGTAT+TGG + chr8.1:4932935-4932954 MS.gene073286:CDS 40.0%
!! ATGGGAGGTATTGGACATTA+TGG + chr8.1:4932944-4932963 MS.gene073286:CDS 40.0%
ACATTTGCAGCTTCAGTTGC+TGG + chr8.1:4931969-4931988 MS.gene073286:CDS 45.0%
ACTACATGCTCAAGAAGGGA+TGG + chr8.1:4932333-4932352 MS.gene073286:CDS 45.0%
ATTGAGTCATCAGAAAGGGG+TGG - chr8.1:4932299-4932318 None:intergenic 45.0%
CAGATGCCAGGATACTATGA+TGG + chr8.1:4932925-4932944 MS.gene073286:CDS 45.0%
CATCATAGTATCCTGGCATC+TGG - chr8.1:4932927-4932946 None:intergenic 45.0%
CATTTGCAGCTTCAGTTGCT+GGG + chr8.1:4931970-4931989 MS.gene073286:CDS 45.0%
GGGCCAAAGATTTGATGAGT+TGG - chr8.1:4932018-4932037 None:intergenic 45.0%
TACCTCCCATCATAGTATCC+TGG - chr8.1:4932934-4932953 None:intergenic 45.0%
!!! TAGTTTTTTTTATTATTATA+AGG + chr8.1:4932415-4932434 MS.gene073286:intron 5.0%
AGAGCAAATAGCCAGATGCC+AGG + chr8.1:4932913-4932932 MS.gene073286:CDS 50.0%
AGTTGGACTTCAAGCCCACA+AGG - chr8.1:4932001-4932020 None:intergenic 50.0%
! TGCCAGGATACTATGATGGG+AGG + chr8.1:4932929-4932948 MS.gene073286:CDS 50.0%
CAAGCGACACATGCAAGCCA+TGG + chr8.1:4933074-4933093 MS.gene073286:CDS 55.0%
GACTTCAAGCCCACAAGGCC+AGG - chr8.1:4931996-4932015 None:intergenic 60.0%
GCAGCTTCAGTTGCTGGGCC+TGG + chr8.1:4931975-4931994 MS.gene073286:CDS 65.0%
!! AGTTGCTGGGCCTGGCCTTG+TGG + chr8.1:4931983-4932002 MS.gene073286:CDS 65.0%
!! GTTGCTGGGCCTGGCCTTGT+GGG + chr8.1:4931984-4932003 MS.gene073286:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 4931957 4933137 4931957 ID=MS.gene073286
chr8.1 mRNA 4931957 4933137 4931957 ID=MS.gene073286.t1;Parent=MS.gene073286
chr8.1 exon 4931957 4932106 4931957 ID=MS.gene073286.t1.exon1;Parent=MS.gene073286.t1
chr8.1 CDS 4931957 4932106 4931957 ID=cds.MS.gene073286.t1;Parent=MS.gene073286.t1
chr8.1 exon 4932248 4932379 4932248 ID=MS.gene073286.t1.exon2;Parent=MS.gene073286.t1
chr8.1 CDS 4932248 4932379 4932248 ID=cds.MS.gene073286.t1;Parent=MS.gene073286.t1
chr8.1 exon 4932898 4933137 4932898 ID=MS.gene073286.t1.exon3;Parent=MS.gene073286.t1
chr8.1 CDS 4932898 4933137 4932898 ID=cds.MS.gene073286.t1;Parent=MS.gene073286.t1
Gene Sequence

>MS.gene073286

ATGTCTGCAGGAACATTTGCAGCTTCAGTTGCTGGGCCTGGCCTTGTGGGCTTGAAGTCCAACTCATCAAATCTTTGGCCCAGTACTACTTATTCCATTGAATGGAAAAAGAAGACTGTCTCCAATGGTTCTAAGATTCACTGCATGAAGGTTAAGTAATCTTATACAATAGCCATATATTATGATAGCATGGTTACATATATGTACTAGTACTCCCATCTTGGTCTTTTTCTTTTTCTTTTCTTTTCTAATATGCTTTGATTTGTGATATAATTTTGTAACTTAAAACAGACATGGAATCCCATTAATAATAGGAAATTTGAAACACTTTCCTATTTACCACCCCTTTCTGATGACTCAATTGCAAAGGAGATTGACTACATGCTCAAGAAGGGATGGACACCTTGCCTTGAATTTGATGAGGTTAGTCCAACCTAAATCTAGTTTATAATATAAGGTAGTTTTTTTTATTATTATAAGGAAAAAACAACTAGGTATACTACTATACTAGAGCTTGTTGATATTGATTCATATAATATAGATTAAGGCAATGTTTGGCTAAACAATTTAATTAAGCACTTATAGCGAAAATTAAAATGAATTCAAATTATTTTCATAGAATCTATAAAGTTATTTTCATAAGCTAATCTAGAGAGCTTATGAAAATAATCTAAAAAAGTTTATTAACATGTCGTAAATTGTTTTCATAAGATTTCTCAAACAATCTTATAGTTTTTATGTCGCTAGATAAACTCATATAAGTTGATATAAACAAACTGTAATAATTTAAAAATATCTATGTCATGATCCAATAAAACTTTTGTATAACTTTAATTTATTCTATGAAGTGGATATTTTTGTTTTTCCTTTTGATGTTTTTGTTTTCTAAGTATTGTATATCTTATTAATAATATGGAACTTGTATTGTTTGGAAATGGAAGTTTGGATGTATTCATAGAGCAAATAGCCAGATGCCAGGATACTATGATGGGAGGTATTGGACATTATGGAAGCTACCAATGTTTGGTTGTAACGACTCTTCACAAGTGCTTAATGAGATTAATGAATGCAAAAAGACATATCCAAATGCATATATAAGATGCTTAGCATTTGACAACAAGCGACACATGCAAGCCATGGCTTTTATTATTCACAAACCTACTACCACAACAACTACTTAA

Protein sequence

>MS.gene073286.t1

MSAGTFAASVAGPGLVGLKSNSSNLWPSTTYSIEWKKKTVSNGSKIHCMKTWNPINNRKFETLSYLPPLSDDSIAKEIDYMLKKGWTPCLEFDEFGCIHRANSQMPGYYDGRYWTLWKLPMFGCNDSSQVLNEINECKKTYPNAYIRCLAFDNKRHMQAMAFIIHKPTTTTTT