Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073300.t1 | XP_003610073.1 | 99.1 | 221 | 2 | 0 | 1 | 221 | 1 | 221 | 8.30E-119 | 436.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073300.t1 | Q9LVD0 | 79.1 | 220 | 46 | 0 | 1 | 220 | 1 | 220 | 1.1e-98 | 360.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073300.t1 | G7JUC5 | 99.1 | 221 | 2 | 0 | 1 | 221 | 1 | 221 | 6.0e-119 | 436.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049207 | MS.gene073300 | 0.809938 | 1.48E-50 | -1.69E-46 |
| MS.gene049441 | MS.gene073300 | 0.80547 | 1.31E-49 | -1.69E-46 |
| MS.gene049473 | MS.gene073300 | 0.822089 | 2.85E-53 | -1.69E-46 |
| MS.gene049580 | MS.gene073300 | 0.823482 | 1.35E-53 | -1.69E-46 |
| MS.gene049824 | MS.gene073300 | 0.889596 | 2.10E-73 | -1.69E-46 |
| MS.gene049825 | MS.gene073300 | 0.895493 | 9.10E-76 | -1.69E-46 |
| MS.gene049873 | MS.gene073300 | 0.873067 | 1.96E-67 | -1.69E-46 |
| MS.gene050277 | MS.gene073300 | 0.824068 | 9.86E-54 | -1.69E-46 |
| MS.gene050303 | MS.gene073300 | 0.830301 | 3.17E-55 | -1.69E-46 |
| MS.gene050427 | MS.gene073300 | 0.851562 | 8.19E-61 | -1.69E-46 |
| MS.gene050916 | MS.gene073300 | 0.803632 | 3.18E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene073300 | 0.814406 | 1.57E-51 | -1.69E-46 |
| MS.gene051791 | MS.gene073300 | 0.81763 | 2.99E-52 | -1.69E-46 |
| MS.gene052947 | MS.gene073300 | 0.837494 | 5.03E-57 | -1.69E-46 |
| MS.gene053122 | MS.gene073300 | 0.840863 | 6.73E-58 | -1.69E-46 |
| MS.gene053195 | MS.gene073300 | 0.800432 | 1.45E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene073300 | 0.825446 | 4.67E-54 | -1.69E-46 |
| MS.gene053294 | MS.gene073300 | 0.846867 | 1.66E-59 | -1.69E-46 |
| MS.gene053401 | MS.gene073300 | 0.8477 | 9.80E-60 | -1.69E-46 |
| MS.gene05365 | MS.gene073300 | 0.810106 | 1.36E-50 | -1.69E-46 |
| MS.gene053995 | MS.gene073300 | 0.82121 | 4.56E-53 | -1.69E-46 |
| MS.gene05548 | MS.gene073300 | 0.819405 | 1.18E-52 | -1.69E-46 |
| MS.gene055555 | MS.gene073300 | 0.862608 | 4.49E-64 | -1.69E-46 |
| MS.gene055808 | MS.gene073300 | 0.882619 | 8.95E-71 | -1.69E-46 |
| MS.gene056075 | MS.gene073300 | 0.829624 | 4.64E-55 | -1.69E-46 |
| MS.gene056091 | MS.gene073300 | 0.882734 | 8.13E-71 | -1.69E-46 |
| MS.gene056403 | MS.gene073300 | 0.805807 | 1.12E-49 | -1.69E-46 |
| MS.gene056605 | MS.gene073300 | 0.805195 | 1.50E-49 | -1.69E-46 |
| MS.gene056631 | MS.gene073300 | 0.839253 | 1.77E-57 | -1.69E-46 |
| MS.gene05689 | MS.gene073300 | 0.831547 | 1.57E-55 | -1.69E-46 |
| MS.gene057705 | MS.gene073300 | 0.85839 | 8.51E-63 | -1.69E-46 |
| MS.gene058030 | MS.gene073300 | 0.800793 | 1.22E-48 | -1.69E-46 |
| MS.gene05953 | MS.gene073300 | 0.84362 | 1.25E-58 | -1.69E-46 |
| MS.gene05954 | MS.gene073300 | 0.854122 | 1.52E-61 | -1.69E-46 |
| MS.gene059935 | MS.gene073300 | 0.861964 | 7.08E-64 | -1.69E-46 |
| MS.gene06001 | MS.gene073300 | 0.849037 | 4.18E-60 | -1.69E-46 |
| MS.gene060429 | MS.gene073300 | 0.845519 | 3.86E-59 | -1.69E-46 |
| MS.gene060586 | MS.gene073300 | 0.801261 | 9.79E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene073300 | 0.814618 | 1.41E-51 | -1.69E-46 |
| MS.gene060830 | MS.gene073300 | 0.847929 | 8.47E-60 | -1.69E-46 |
| MS.gene061266 | MS.gene073300 | 0.804877 | 1.75E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073300.t1 | MTR_4g127640 | 98.729 | 236 | 3 | 0 | 1 | 236 | 1 | 236 | 8.27e-172 | 474 |
| MS.gene073300.t1 | MTR_4g127640 | 94.915 | 236 | 3 | 1 | 1 | 236 | 1 | 227 | 3.34e-162 | 450 |
| MS.gene073300.t1 | MTR_4g127640 | 96.703 | 182 | 6 | 0 | 55 | 236 | 64 | 245 | 2.10e-127 | 362 |
| MS.gene073300.t1 | MTR_4g007420 | 71.186 | 236 | 62 | 2 | 1 | 236 | 1 | 230 | 5.87e-120 | 343 |
| MS.gene073300.t1 | MTR_4g007390 | 75.410 | 61 | 15 | 0 | 1 | 61 | 1 | 61 | 2.45e-25 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073300.t1 | AT5G57280 | 77.021 | 235 | 53 | 1 | 1 | 235 | 1 | 234 | 1.85e-133 | 377 |
Find 54 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTTTGGTTTCAGGGTTT+GGG | 0.166055 | 8.1:+5152815 | MS.gene073300:intron |
| ACCTATGTCAAGGAGTAATT+TGG | 0.173913 | 8.1:-5152088 | None:intergenic |
| TTCCCGTATTATCGATATTC+AGG | 0.260528 | 8.1:+5151929 | MS.gene073300:CDS |
| CTTCGTCCTGGAGTTATTGA+TGG | 0.312897 | 8.1:+5152839 | MS.gene073300:CDS |
| AGAAAAGAGTTTCTTGTTCT+CGG | 0.339903 | 8.1:+5165566 | MS.gene073300:CDS |
| TACATCTTTATACAGATGTT+TGG | 0.363170 | 8.1:+5153389 | MS.gene073300:CDS |
| CATCAGTATATCTGCCGTTC+AGG | 0.376368 | 8.1:+5152865 | MS.gene073300:CDS |
| TGCATTGGAGCGAGAGGTTG+AGG | 0.387646 | 8.1:+5152563 | MS.gene073300:CDS |
| CAGGGTTTGGGGCTTCGTCC+TGG | 0.388372 | 8.1:+5152827 | MS.gene073300:intron |
| GAGTTTCTTGTTCTCGGATG+TGG | 0.418174 | 8.1:+5165572 | MS.gene073300:CDS |
| CCTATGTCAAGGAGTAATTT+GGG | 0.418574 | 8.1:-5152087 | None:intergenic |
| GAACCTGAATATCGATAATA+CGG | 0.420565 | 8.1:-5151932 | None:intergenic |
| AATTTGGGAACACCATCTTC+AGG | 0.421843 | 8.1:-5152072 | None:intergenic |
| TTCGTCCTGGAGTTATTGAT+GGG | 0.440275 | 8.1:+5152840 | MS.gene073300:CDS |
| ATCTTTGGTTTCAGGGTTTG+GGG | 0.457597 | 8.1:+5152816 | MS.gene073300:intron |
| TTCATCTTCGCTCTCTTCAT+CGG | 0.463844 | 8.1:-5165654 | None:intergenic |
| TTTACTCAGTGACATGGGTC+AGG | 0.470372 | 8.1:+5152593 | MS.gene073300:CDS |
| GAGTGTCTCACCACTGAGTC+CGG | 0.480074 | 8.1:-5152195 | None:intergenic |
| ATTTGTGTAGGTTGTGGATC+CGG | 0.495040 | 8.1:+5152176 | MS.gene073300:intron |
| TCAGGTGGTGCCACCCGCTC+CGG | 0.495539 | 8.1:-5151864 | None:intergenic |
| GAAAACATACTAAGCATTGA+AGG | 0.495940 | 8.1:-5152257 | None:intergenic |
| TTTAGCGATGGCGTCTCGAC+CGG | 0.500937 | 8.1:+5151845 | None:intergenic |
| TTGAATGCTGCAATGCATGC+TGG | 0.505367 | 8.1:+5153474 | MS.gene073300:CDS |
| GATGGCGTCTCGACCGGAGC+GGG | 0.514067 | 8.1:+5151851 | None:intergenic |
| AGAAGTGTACTTGCGAGCTT+CGG | 0.519171 | 8.1:-5151904 | None:intergenic |
| GTATCATCGTAGAATATCTC+AGG | 0.524688 | 8.1:-5151882 | None:intergenic |
| TTATACAGATGTTTGGCCAA+TGG | 0.529283 | 8.1:+5153396 | MS.gene073300:CDS |
| TCTCAGAGGAAGGTCATCAA+TGG | 0.533812 | 8.1:+5152216 | MS.gene073300:CDS |
| CTCACTCTCAGAACGAGCAT+TGG | 0.536619 | 8.1:+5152035 | MS.gene073300:CDS |
| TTAACTCGTAGACATTGCAT+TGG | 0.536621 | 8.1:+5152548 | MS.gene073300:intron |
| CTTCTCGCTTTACCTGAAGA+TGG | 0.538980 | 8.1:+5152060 | MS.gene073300:CDS |
| GAGGAAGGTCATCAATGGAT+TGG | 0.539136 | 8.1:+5152221 | MS.gene073300:CDS |
| GGCGTCTCGACCGGAGCGGG+TGG | 0.542531 | 8.1:+5151854 | MS.gene073300:CDS |
| GCATTGGAGCGAGAGGTTGA+GGG | 0.553379 | 8.1:+5152564 | MS.gene073300:CDS |
| CCCAAATTACTCCTTGACAT+AGG | 0.568900 | 8.1:+5152087 | MS.gene073300:CDS |
| GAAATATAATACCTATGTCA+AGG | 0.583040 | 8.1:-5152098 | None:intergenic |
| CAATGCTCGTTCTGAGAGTG+AGG | 0.587056 | 8.1:-5152034 | None:intergenic |
| ATAGATCTTCTCACAACCCT+CGG | 0.590662 | 8.1:+5153153 | MS.gene073300:CDS |
| ATGGCCCCATCAATAACTCC+AGG | 0.600380 | 8.1:-5152845 | None:intergenic |
| AGACATTGCATTGGAGCGAG+AGG | 0.633920 | 8.1:+5152557 | MS.gene073300:intron |
| ACGAAAGCATCCTTGTCCAA+AGG | 0.634791 | 8.1:+5165605 | MS.gene073300:CDS |
| CATACTTCAATCTTAACCGA+GGG | 0.636905 | 8.1:-5153169 | None:intergenic |
| GGTGAGACACTCTCAGAGGA+AGG | 0.638568 | 8.1:+5152206 | MS.gene073300:CDS |
| AACCTGAATATCGATAATAC+GGG | 0.644042 | 8.1:-5151931 | None:intergenic |
| CGATGGCGTCTCGACCGGAG+CGG | 0.660378 | 8.1:+5151850 | None:intergenic |
| ACATACTTCAATCTTAACCG+AGG | 0.662003 | 8.1:-5153170 | None:intergenic |
| CTGAACGGCAGATATACTGA+TGG | 0.670431 | 8.1:-5152864 | None:intergenic |
| TCGTCCTGGAGTTATTGATG+GGG | 0.672155 | 8.1:+5152841 | MS.gene073300:CDS |
| TTATCGAAAGCATACCTGAA+CGG | 0.672886 | 8.1:-5152879 | None:intergenic |
| TCATCGTAGAATATCTCAGG+TGG | 0.679096 | 8.1:-5151879 | None:intergenic |
| CATTCAAAATCAATTCACGC+TGG | 0.680289 | 8.1:-5153458 | None:intergenic |
| AAGATTTATTCATACCTGTG+TGG | 0.684315 | 8.1:-5153525 | None:intergenic |
| CAGTGGTGAGACACTCTCAG+AGG | 0.699390 | 8.1:+5152202 | MS.gene073300:CDS |
| GGTTGTGGATCCGGACTCAG+TGG | 0.702736 | 8.1:+5152185 | MS.gene073300:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 5151852 | 5165688 | 5151852 | ID=MS.gene073300 |
| chr8.1 | mRNA | 5151852 | 5165688 | 5151852 | ID=MS.gene073300.t1;Parent=MS.gene073300 |
| chr8.1 | exon | 5151852 | 5151950 | 5151852 | ID=MS.gene073300.t1.exon1;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5151852 | 5151950 | 5151852 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| chr8.1 | exon | 5152033 | 5152108 | 5152033 | ID=MS.gene073300.t1.exon2;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5152033 | 5152108 | 5152033 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| chr8.1 | exon | 5152186 | 5152269 | 5152186 | ID=MS.gene073300.t1.exon3;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5152186 | 5152269 | 5152186 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| chr8.1 | exon | 5152559 | 5152614 | 5152559 | ID=MS.gene073300.t1.exon4;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5152559 | 5152614 | 5152559 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| chr8.1 | exon | 5152830 | 5152886 | 5152830 | ID=MS.gene073300.t1.exon5;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5152830 | 5152886 | 5152830 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| chr8.1 | exon | 5153137 | 5153186 | 5153137 | ID=MS.gene073300.t1.exon6;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5153137 | 5153186 | 5153137 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| chr8.1 | exon | 5153380 | 5153532 | 5153380 | ID=MS.gene073300.t1.exon7;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5153380 | 5153532 | 5153380 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| chr8.1 | exon | 5165556 | 5165688 | 5165556 | ID=MS.gene073300.t1.exon8;Parent=MS.gene073300.t1 |
| chr8.1 | CDS | 5165556 | 5165688 | 5165556 | ID=cds.MS.gene073300.t1;Parent=MS.gene073300.t1 |
| Gene Sequence |
| Protein sequence |
