AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07636


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07636.t1 MTR_3g064020 84.615 247 35 1 1 244 1 247 3.40e-155 431
MS.gene07636.t1 MTR_5g076250 62.500 248 77 6 1 239 1 241 1.32e-95 280
MS.gene07636.t1 MTR_3g063990 54.980 251 71 4 9 221 3 249 8.25e-84 251
MS.gene07636.t1 MTR_1g057650 51.653 242 103 6 1 236 1 234 1.01e-73 225
MS.gene07636.t1 MTR_7g117750 43.983 241 121 5 1 236 1 232 1.74e-60 191
MS.gene07636.t1 MTR_3g063980 56.164 73 28 1 167 239 56 124 2.01e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07636.t1 AT3G02700 57.917 240 89 6 1 236 1 232 3.14e-81 244
MS.gene07636.t1 AT5G06370 50.826 242 105 6 1 236 1 234 8.77e-74 225
MS.gene07636.t1 AT5G16360 47.101 276 96 8 1 236 1 266 5.11e-70 216
MS.gene07636.t1 AT4G00905 45.714 245 120 5 1 236 1 241 5.87e-63 197
MS.gene07636.t1 AT5G16330 47.325 243 98 8 1 236 9 228 7.72e-62 194
MS.gene07636.t1 AT1G01225 47.391 230 112 3 1 226 1 225 1.82e-61 194

Find 56 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTAGATTGCTTTCTTTC+AGG 0.124117 3.2:+59580642 MS.gene07636:CDS
GCTTTATATCTTCTTGAAAA+TGG 0.152944 3.2:+59580780 MS.gene07636:CDS
AAAAGATTGGTTTCTGATAT+TGG 0.189791 3.2:+59581008 MS.gene07636:CDS
ATGTTTCCGATCAGTCTTAT+TGG 0.247318 3.2:-59580232 None:intergenic
CACAGTTATTCTTGAAGATA+TGG 0.269187 3.2:-59580815 None:intergenic
TATCTTCTTGAAAATGGATT+TGG 0.299009 3.2:+59580786 MS.gene07636:CDS
TTTGCAGTTTATTGCAAAAC+AGG 0.337490 3.2:+59580843 MS.gene07636:CDS
TGCATGTGCCTCCTCTAGCT+TGG 0.338272 3.2:-59580716 None:intergenic
CCGCACCGTCACGACATCTT+TGG 0.342473 3.2:-59580590 None:intergenic
GAACTCTACCTCTTTCAATA+TGG 0.373949 3.2:+59580669 MS.gene07636:CDS
AACTTACTGCATTAAAAGAT+TGG 0.377765 3.2:+59580995 MS.gene07636:CDS
ATGGTGATCCATTTCACAAT+TGG 0.385236 3.2:+59580489 MS.gene07636:CDS
CTGGGGATCATATTTACTCT+TGG 0.413378 3.2:+59580262 MS.gene07636:CDS
CTTCCATCCATGGTCAAATC+AGG 0.416092 3.2:-59581083 None:intergenic
GCATGTGCCTCCTCTAGCTT+GGG 0.419205 3.2:-59580715 None:intergenic
CACATGCACCCTAGCTGCTT+CGG 0.423293 3.2:+59580731 MS.gene07636:CDS
ATCCATTTCACAATTGGATC+AGG 0.464773 3.2:+59580495 MS.gene07636:CDS
ATCGGAAACATCTAAAACCT+GGG 0.479101 3.2:+59580244 MS.gene07636:CDS
GGGATCATATTTACTCTTGG+AGG 0.483005 3.2:+59580265 MS.gene07636:CDS
ACAGGAATATATGTTGAAGA+TGG 0.483154 3.2:+59580465 MS.gene07636:intron
GTCCTGATCCAATTGTGAAA+TGG 0.487607 3.2:-59580497 None:intergenic
GGATCAGGACAGCAAGCTGC+TGG 0.487944 3.2:+59580510 MS.gene07636:CDS
CGAAAGAACTTGTAAAGAAA+CGG 0.498819 3.2:-59580551 None:intergenic
TCATATTTACTCTTGGAGGC+AGG 0.503930 3.2:+59580269 MS.gene07636:CDS
ATACCTGATTTGACCATGGA+TGG 0.505525 3.2:+59581080 MS.gene07636:CDS
GCTGGCAGCAGCTGAATAAG+AGG 0.511661 3.2:-59580904 None:intergenic
TGAAAGAAAGCAATCTAAGC+AGG 0.512899 3.2:-59580640 None:intergenic
ACATCTTTGGCATGGAATGT+TGG 0.518788 3.2:-59580577 None:intergenic
TTCAAGAAGATATAAAGCAC+GGG 0.519285 3.2:-59580775 None:intergenic
TGGAGACACCATATTGAAAG+AGG 0.529495 3.2:-59580677 None:intergenic
CAGTTGGATCCGAAGCAGCT+AGG 0.530992 3.2:-59580740 None:intergenic
AGTTGGATCCGAAGCAGCTA+GGG 0.533238 3.2:-59580739 None:intergenic
GTCGTGACGGTGCGGAAACA+AGG 0.535193 3.2:+59580598 MS.gene07636:CDS
AGTCTACTAGCTTCCATCCA+TGG 0.540139 3.2:-59581093 None:intergenic
GATCGGAAACATCTAAAACC+TGG 0.543021 3.2:+59580243 MS.gene07636:CDS
AAGAAACGGTATAAAATCGC+TGG 0.543391 3.2:-59580537 None:intergenic
TTTCAAGAAGATATAAAGCA+CGG 0.545122 3.2:-59580776 None:intergenic
CGGGGAAGGACCTCTTCAGT+TGG 0.547650 3.2:-59580756 None:intergenic
GGTAGGGCTTTGGTTTGCTG+TGG 0.552956 3.2:+59580972 MS.gene07636:CDS
TCAACCTCAAAAGGCTGCAG+TGG 0.553525 3.2:+59580873 MS.gene07636:CDS
TTAGATTGCTTTCTTTCAGG+AGG 0.554330 3.2:+59580645 MS.gene07636:CDS
TGCTTTCCAATAAGACTGAT+CGG 0.558642 3.2:+59580226 MS.gene07636:CDS
TGATGAAGAGTCTACAGTGC+TGG 0.563974 3.2:-59580922 None:intergenic
CAAGAGTAAATATGATCCCC+AGG 0.568302 3.2:-59580261 None:intergenic
CTGTAGACTCTTCATCACTT+CGG 0.584097 3.2:+59580928 MS.gene07636:CDS
CCGTCACGACATCTTTGGCA+TGG 0.598030 3.2:-59580585 None:intergenic
GAAGATATAAAGCACGGGGA+AGG 0.600493 3.2:-59580770 None:intergenic
AATATATGTTGAAGATGGAA+TGG 0.601381 3.2:+59580470 MS.gene07636:CDS
GCTTACCTCTATGCACATCA+TGG 0.604488 3.2:+59580291 MS.gene07636:CDS
GGTGCGGAAACAAGGACTCA+TGG 0.614117 3.2:+59580606 MS.gene07636:CDS
GATGACCATGATGTGCATAG+AGG 0.641641 3.2:-59580296 None:intergenic
TGCTTCGGATCCAACTGAAG+AGG 0.647462 3.2:+59580746 MS.gene07636:CDS
CCAAAGATGTCGTGACGGTG+CGG 0.654870 3.2:+59580590 MS.gene07636:CDS
CCATGCCAAAGATGTCGTGA+CGG 0.658966 3.2:+59580585 MS.gene07636:CDS
TCAAGAAGATATAAAGCACG+GGG 0.677622 3.2:-59580774 None:intergenic
TCGGAAACATCTAAAACCTG+GGG 0.712111 3.2:+59580245 MS.gene07636:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTTGATACATAAGAAAA+TGG - chr3.2:59580374-59580393 None:intergenic 20.0%
! AACTTACTGCATTAAAAGAT+TGG + chr3.2:59580995-59581014 MS.gene07636:CDS 25.0%
! AATATATGTTGAAGATGGAA+TGG + chr3.2:59580470-59580489 MS.gene07636:CDS 25.0%
! GCTTTATATCTTCTTGAAAA+TGG + chr3.2:59580780-59580799 MS.gene07636:CDS 25.0%
! TTTCAAGAAGATATAAAGCA+CGG - chr3.2:59580779-59580798 None:intergenic 25.0%
!! TATCTTCTTGAAAATGGATT+TGG + chr3.2:59580786-59580805 MS.gene07636:CDS 25.0%
!! TTGTGCAAGAAAGTATAAAT+TGG - chr3.2:59580344-59580363 None:intergenic 25.0%
!!! AAAAGATTGGTTTCTGATAT+TGG + chr3.2:59581008-59581027 MS.gene07636:CDS 25.0%
!!! AAATCAGGTATTTTTTCAAC+TGG - chr3.2:59581071-59581090 None:intergenic 25.0%
ACAGGAATATATGTTGAAGA+TGG + chr3.2:59580465-59580484 MS.gene07636:intron 30.0%
CACAGTTATTCTTGAAGATA+TGG - chr3.2:59580818-59580837 None:intergenic 30.0%
CGAAAGAACTTGTAAAGAAA+CGG - chr3.2:59580554-59580573 None:intergenic 30.0%
TCAGTATGATAGTTATGCAT+AGG - chr3.2:59580399-59580418 None:intergenic 30.0%
TTCAAGAAGATATAAAGCAC+GGG - chr3.2:59580778-59580797 None:intergenic 30.0%
TTGGGCTAAAAAATGTAGTT+TGG - chr3.2:59580700-59580719 None:intergenic 30.0%
TTTGCAGTTTATTGCAAAAC+AGG + chr3.2:59580843-59580862 MS.gene07636:CDS 30.0%
! AAAAATACCTGATTTGACCA+TGG + chr3.2:59581076-59581095 MS.gene07636:CDS 30.0%
AAGAAACGGTATAAAATCGC+TGG - chr3.2:59580540-59580559 None:intergenic 35.0%
ATCCATTTCACAATTGGATC+AGG + chr3.2:59580495-59580514 MS.gene07636:CDS 35.0%
ATGGTGATCCATTTCACAAT+TGG + chr3.2:59580489-59580508 MS.gene07636:CDS 35.0%
CGGCATTTCTGTAATATAAC+AGG + chr3.2:59580447-59580466 MS.gene07636:intron 35.0%
GAACTCTACCTCTTTCAATA+TGG + chr3.2:59580669-59580688 MS.gene07636:CDS 35.0%
TCAAGAAGATATAAAGCACG+GGG - chr3.2:59580777-59580796 None:intergenic 35.0%
TCATATTTGTGTCCTGCTAA+CGG + chr3.2:59580427-59580446 MS.gene07636:intron 35.0%
TGAAAGAAAGCAATCTAAGC+AGG - chr3.2:59580643-59580662 None:intergenic 35.0%
TGCTTAGATTGCTTTCTTTC+AGG + chr3.2:59580642-59580661 MS.gene07636:CDS 35.0%
TGCTTTCCAATAAGACTGAT+CGG + chr3.2:59580226-59580245 MS.gene07636:CDS 35.0%
TTAGATTGCTTTCTTTCAGG+AGG + chr3.2:59580645-59580664 MS.gene07636:CDS 35.0%
! ATCGGAAACATCTAAAACCT+GGG + chr3.2:59580244-59580263 MS.gene07636:CDS 35.0%
! ATGTTTCCGATCAGTCTTAT+TGG - chr3.2:59580235-59580254 None:intergenic 35.0%
! TTTGTGAACTCCAGTCTTTT+TGG + chr3.2:59580951-59580970 MS.gene07636:CDS 35.0%
!!! GGTCTTTTTTCAACCTCAAA+AGG + chr3.2:59580864-59580883 MS.gene07636:CDS 35.0%
ACATCTTTGGCATGGAATGT+TGG - chr3.2:59580580-59580599 None:intergenic 40.0%
ATTACAGAAATGCCGTTAGC+AGG - chr3.2:59580442-59580461 None:intergenic 40.0%
CAAGAGTAAATATGATCCCC+AGG - chr3.2:59580264-59580283 None:intergenic 40.0%
CTGGGGATCATATTTACTCT+TGG + chr3.2:59580262-59580281 MS.gene07636:CDS 40.0%
CTGTAGACTCTTCATCACTT+CGG + chr3.2:59580928-59580947 MS.gene07636:CDS 40.0%
GGGATCATATTTACTCTTGG+AGG + chr3.2:59580265-59580284 MS.gene07636:CDS 40.0%
GTCCTGATCCAATTGTGAAA+TGG - chr3.2:59580500-59580519 None:intergenic 40.0%
TCATATTTACTCTTGGAGGC+AGG + chr3.2:59580269-59580288 MS.gene07636:CDS 40.0%
TGGAGACACCATATTGAAAG+AGG - chr3.2:59580680-59580699 None:intergenic 40.0%
! ATACCTGATTTGACCATGGA+TGG + chr3.2:59581080-59581099 MS.gene07636:CDS 40.0%
! GATCGGAAACATCTAAAACC+TGG + chr3.2:59580243-59580262 MS.gene07636:CDS 40.0%
! TCGGAAACATCTAAAACCTG+GGG + chr3.2:59580245-59580264 MS.gene07636:CDS 40.0%
!! GAACTCCAGTCTTTTTGGTA+GGG + chr3.2:59580956-59580975 MS.gene07636:CDS 40.0%
!! TGAACTCCAGTCTTTTTGGT+AGG + chr3.2:59580955-59580974 MS.gene07636:CDS 40.0%
!!! CATTTTTTAGCCCAAGCTAG+AGG + chr3.2:59580705-59580724 MS.gene07636:CDS 40.0%
CAAAGCCCTACCAAAAAGAC+TGG - chr3.2:59580964-59580983 None:intergenic 45.0%
CTTCCATCCATGGTCAAATC+AGG - chr3.2:59581086-59581105 None:intergenic 45.0%
GAAGATATAAAGCACGGGGA+AGG - chr3.2:59580773-59580792 None:intergenic 45.0%
GATGACCATGATGTGCATAG+AGG - chr3.2:59580299-59580318 None:intergenic 45.0%
GCTTACCTCTATGCACATCA+TGG + chr3.2:59580291-59580310 MS.gene07636:CDS 45.0%
TGATGAAGAGTCTACAGTGC+TGG - chr3.2:59580925-59580944 None:intergenic 45.0%
!! CAGTCTTTTTGGTAGGGCTT+TGG + chr3.2:59580962-59580981 MS.gene07636:CDS 45.0%
!! TTTTTAGCCCAAGCTAGAGG+AGG + chr3.2:59580708-59580727 MS.gene07636:CDS 45.0%
AGTTGGATCCGAAGCAGCTA+GGG - chr3.2:59580742-59580761 None:intergenic 50.0%
CCATGCCAAAGATGTCGTGA+CGG + chr3.2:59580585-59580604 MS.gene07636:CDS 50.0%
TCAACCTCAAAAGGCTGCAG+TGG + chr3.2:59580873-59580892 MS.gene07636:CDS 50.0%
TGCTTCGGATCCAACTGAAG+AGG + chr3.2:59580746-59580765 MS.gene07636:CDS 50.0%
! TTGACCACTGCAGCCTTTTG+AGG - chr3.2:59580880-59580899 None:intergenic 50.0%
CACATGCACCCTAGCTGCTT+CGG + chr3.2:59580731-59580750 MS.gene07636:CDS 55.0%
CAGTTGGATCCGAAGCAGCT+AGG - chr3.2:59580743-59580762 None:intergenic 55.0%
CCAAAGATGTCGTGACGGTG+CGG + chr3.2:59580590-59580609 MS.gene07636:CDS 55.0%
CCGTCACGACATCTTTGGCA+TGG - chr3.2:59580588-59580607 None:intergenic 55.0%
GCTGGCAGCAGCTGAATAAG+AGG - chr3.2:59580907-59580926 None:intergenic 55.0%
GGTGCGGAAACAAGGACTCA+TGG + chr3.2:59580606-59580625 MS.gene07636:CDS 55.0%
TGCATGTGCCTCCTCTAGCT+TGG - chr3.2:59580719-59580738 None:intergenic 55.0%
! GCATGTGCCTCCTCTAGCTT+GGG - chr3.2:59580718-59580737 None:intergenic 55.0%
!! GGTAGGGCTTTGGTTTGCTG+TGG + chr3.2:59580972-59580991 MS.gene07636:CDS 55.0%
CCGCACCGTCACGACATCTT+TGG - chr3.2:59580593-59580612 None:intergenic 60.0%
CGGGGAAGGACCTCTTCAGT+TGG - chr3.2:59580759-59580778 None:intergenic 60.0%
GGATCAGGACAGCAAGCTGC+TGG + chr3.2:59580510-59580529 MS.gene07636:CDS 60.0%
GTCGTGACGGTGCGGAAACA+AGG + chr3.2:59580598-59580617 MS.gene07636:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 59580219 59581109 59580219 ID=MS.gene07636
chr3.2 mRNA 59580219 59581109 59580219 ID=MS.gene07636.t1;Parent=MS.gene07636
chr3.2 exon 59580219 59580312 59580219 ID=MS.gene07636.t1.exon1;Parent=MS.gene07636.t1
chr3.2 CDS 59580219 59580312 59580219 ID=cds.MS.gene07636.t1;Parent=MS.gene07636.t1
chr3.2 exon 59580469 59581109 59580469 ID=MS.gene07636.t1.exon2;Parent=MS.gene07636.t1
chr3.2 CDS 59580469 59581109 59580469 ID=cds.MS.gene07636.t1;Parent=MS.gene07636.t1
Gene Sequence

>MS.gene07636

ATGGGAGTGCTTTCCAATAAGACTGATCGGAAACATCTAAAACCTGGGGATCATATTTACTCTTGGAGGCAGGCTTACCTCTATGCACATCATGGTCATCTCTTTTCTCTTATATTTATCTGCCAATTTATACTTTCTTGCACAACTATGAACCATTTTCTTATGTATCAAACTTATCCTATGCATAACTATCATACTGATGTTAATGTCATATTTGTGTCCTGCTAACGGCATTTCTGTAATATAACAGGAATATATGTTGAAGATGGAATGGTGATCCATTTCACAATTGGATCAGGACAGCAAGCTGCTGGAACACCAGCGATTTTATACCGTTTCTTTACAAGTTCTTTCGATGCCAACATTCCATGCCAAAGATGTCGTGACGGTGCGGAAACAAGGACTCATGGTGTCTTCTCATCCTGCTTAGATTGCTTTCTTTCAGGAGGTGAACTCTACCTCTTTCAATATGGTGTCTCCAAACTACATTTTTTAGCCCAAGCTAGAGGAGGCACATGCACCCTAGCTGCTTCGGATCCAACTGAAGAGGTCCTTCCCCGTGCTTTATATCTTCTTGAAAATGGATTTGGTGACTACCATATCTTCAAGAATAACTGTGAAGATTTTGCAGTTTATTGCAAAACAGGTCTTTTTTCAACCTCAAAAGGCTGCAGTGGTCAAGCAGCCTCTTATTCAGCTGCTGCCAGCACTGTAGACTCTTCATCACTTCGGTTTGTGAACTCCAGTCTTTTTGGTAGGGCTTTGGTTTGCTGTGGAACTTACTGCATTAAAAGATTGGTTTCTGATATTGGATTTCGTTATGAAGTAATTAAAGATGTAACTAAAGTTCCAGTTGAAAAAATACCTGATTTGACCATGGATGGAAGCTAG

Protein sequence

>MS.gene07636.t1

MGVLSNKTDRKHLKPGDHIYSWRQAYLYAHHGIYVEDGMVIHFTIGSGQQAAGTPAILYRFFTSSFDANIPCQRCRDGAETRTHGVFSSCLDCFLSGGELYLFQYGVSKLHFLAQARGGTCTLAASDPTEEVLPRALYLLENGFGDYHIFKNNCEDFAVYCKTGLFSTSKGCSGQAASYSAAASTVDSSSLRFVNSSLFGRALVCCGTYCIKRLVSDIGFRYEVIKDVTKVPVEKIPDLTMDGS