AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049657


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049657.t1 MTR_3g064020 84.615 247 35 1 1 244 1 247 3.40e-155 431
MS.gene049657.t1 MTR_5g076250 62.500 248 77 6 1 239 1 241 1.32e-95 280
MS.gene049657.t1 MTR_3g063990 54.980 251 71 4 9 221 3 249 8.25e-84 251
MS.gene049657.t1 MTR_1g057650 51.653 242 103 6 1 236 1 234 1.01e-73 225
MS.gene049657.t1 MTR_7g117750 43.983 241 121 5 1 236 1 232 1.74e-60 191
MS.gene049657.t1 MTR_3g063980 56.164 73 28 1 167 239 56 124 2.01e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049657.t1 AT3G02700 57.917 240 89 6 1 236 1 232 3.14e-81 244
MS.gene049657.t1 AT5G06370 50.826 242 105 6 1 236 1 234 8.77e-74 225
MS.gene049657.t1 AT5G16360 47.101 276 96 8 1 236 1 266 5.11e-70 216
MS.gene049657.t1 AT4G00905 45.714 245 120 5 1 236 1 241 5.87e-63 197
MS.gene049657.t1 AT5G16330 47.325 243 98 8 1 236 9 228 7.72e-62 194
MS.gene049657.t1 AT1G01225 47.391 230 112 3 1 226 1 225 1.82e-61 194

Find 56 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTAGATTGCTTTCTTTC+AGG 0.124117 3.2:+71604885 MS.gene049657:CDS
GCTTTATATCTTCTTGAAAA+TGG 0.152944 3.2:+71605023 MS.gene049657:CDS
AAAAGATTGGTTTCTGATAT+TGG 0.189791 3.2:+71605251 MS.gene049657:CDS
ATGTTTCCGATCAGTCTTAT+TGG 0.247318 3.2:-71604475 None:intergenic
CACAGTTATTCTTGAAGATA+TGG 0.269187 3.2:-71605058 None:intergenic
TATCTTCTTGAAAATGGATT+TGG 0.299009 3.2:+71605029 MS.gene049657:CDS
TTTGCAGTTTATTGCAAAAC+AGG 0.337490 3.2:+71605086 MS.gene049657:CDS
TGCATGTGCCTCCTCTAGCT+TGG 0.338272 3.2:-71604959 None:intergenic
CCGCACCGTCACGACATCTT+TGG 0.342473 3.2:-71604833 None:intergenic
GAACTCTACCTCTTTCAATA+TGG 0.373949 3.2:+71604912 MS.gene049657:CDS
AACTTACTGCATTAAAAGAT+TGG 0.377765 3.2:+71605238 MS.gene049657:CDS
ATGGTGATCCATTTCACAAT+TGG 0.385236 3.2:+71604732 MS.gene049657:CDS
CTGGGGATCATATTTACTCT+TGG 0.413378 3.2:+71604505 MS.gene049657:CDS
CTTCCATCCATGGTCAAATC+AGG 0.416092 3.2:-71605326 None:intergenic
GCATGTGCCTCCTCTAGCTT+GGG 0.419205 3.2:-71604958 None:intergenic
CACATGCACCCTAGCTGCTT+CGG 0.423293 3.2:+71604974 MS.gene049657:CDS
ATCCATTTCACAATTGGATC+AGG 0.464773 3.2:+71604738 MS.gene049657:CDS
ATCGGAAACATCTAAAACCT+GGG 0.479101 3.2:+71604487 MS.gene049657:CDS
GGGATCATATTTACTCTTGG+AGG 0.483005 3.2:+71604508 MS.gene049657:CDS
ACAGGAATATATGTTGAAGA+TGG 0.483154 3.2:+71604708 MS.gene049657:intron
GTCCTGATCCAATTGTGAAA+TGG 0.487607 3.2:-71604740 None:intergenic
GGATCAGGACAGCAAGCTGC+TGG 0.487944 3.2:+71604753 MS.gene049657:CDS
CGAAAGAACTTGTAAAGAAA+CGG 0.498819 3.2:-71604794 None:intergenic
TCATATTTACTCTTGGAGGC+AGG 0.503930 3.2:+71604512 MS.gene049657:CDS
ATACCTGATTTGACCATGGA+TGG 0.505525 3.2:+71605323 MS.gene049657:CDS
GCTGGCAGCAGCTGAATAAG+AGG 0.511661 3.2:-71605147 None:intergenic
TGAAAGAAAGCAATCTAAGC+AGG 0.512899 3.2:-71604883 None:intergenic
ACATCTTTGGCATGGAATGT+TGG 0.518788 3.2:-71604820 None:intergenic
TTCAAGAAGATATAAAGCAC+GGG 0.519285 3.2:-71605018 None:intergenic
TGGAGACACCATATTGAAAG+AGG 0.529495 3.2:-71604920 None:intergenic
CAGTTGGATCCGAAGCAGCT+AGG 0.530992 3.2:-71604983 None:intergenic
AGTTGGATCCGAAGCAGCTA+GGG 0.533238 3.2:-71604982 None:intergenic
GTCGTGACGGTGCGGAAACA+AGG 0.535193 3.2:+71604841 MS.gene049657:CDS
AGTCTACTAGCTTCCATCCA+TGG 0.540139 3.2:-71605336 None:intergenic
GATCGGAAACATCTAAAACC+TGG 0.543021 3.2:+71604486 MS.gene049657:CDS
AAGAAACGGTATAAAATCGC+TGG 0.543391 3.2:-71604780 None:intergenic
TTTCAAGAAGATATAAAGCA+CGG 0.545122 3.2:-71605019 None:intergenic
CGGGGAAGGACCTCTTCAGT+TGG 0.547650 3.2:-71604999 None:intergenic
GGTAGGGCTTTGGTTTGCTG+TGG 0.552956 3.2:+71605215 MS.gene049657:CDS
TCAACCTCAAAAGGCTGCAG+TGG 0.553525 3.2:+71605116 MS.gene049657:CDS
TTAGATTGCTTTCTTTCAGG+AGG 0.554330 3.2:+71604888 MS.gene049657:CDS
TGCTTTCCAATAAGACTGAT+CGG 0.558642 3.2:+71604469 MS.gene049657:CDS
TGATGAAGAGTCTACAGTGC+TGG 0.563974 3.2:-71605165 None:intergenic
CAAGAGTAAATATGATCCCC+AGG 0.568302 3.2:-71604504 None:intergenic
CTGTAGACTCTTCATCACTT+CGG 0.584097 3.2:+71605171 MS.gene049657:CDS
CCGTCACGACATCTTTGGCA+TGG 0.598030 3.2:-71604828 None:intergenic
GAAGATATAAAGCACGGGGA+AGG 0.600493 3.2:-71605013 None:intergenic
AATATATGTTGAAGATGGAA+TGG 0.601381 3.2:+71604713 MS.gene049657:CDS
GCTTACCTCTATGCACATCA+TGG 0.604488 3.2:+71604534 MS.gene049657:CDS
GGTGCGGAAACAAGGACTCA+TGG 0.614117 3.2:+71604849 MS.gene049657:CDS
GATGACCATGATGTGCATAG+AGG 0.641641 3.2:-71604539 None:intergenic
TGCTTCGGATCCAACTGAAG+AGG 0.647462 3.2:+71604989 MS.gene049657:CDS
CCAAAGATGTCGTGACGGTG+CGG 0.654870 3.2:+71604833 MS.gene049657:CDS
CCATGCCAAAGATGTCGTGA+CGG 0.658966 3.2:+71604828 MS.gene049657:CDS
TCAAGAAGATATAAAGCACG+GGG 0.677622 3.2:-71605017 None:intergenic
TCGGAAACATCTAAAACCTG+GGG 0.712111 3.2:+71604488 MS.gene049657:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTTGATACATAAGAAAA+TGG - chr3.2:71604617-71604636 None:intergenic 20.0%
! AACTTACTGCATTAAAAGAT+TGG + chr3.2:71605238-71605257 MS.gene049657:CDS 25.0%
! AATATATGTTGAAGATGGAA+TGG + chr3.2:71604713-71604732 MS.gene049657:CDS 25.0%
! GCTTTATATCTTCTTGAAAA+TGG + chr3.2:71605023-71605042 MS.gene049657:CDS 25.0%
! TTTCAAGAAGATATAAAGCA+CGG - chr3.2:71605022-71605041 None:intergenic 25.0%
!! TATCTTCTTGAAAATGGATT+TGG + chr3.2:71605029-71605048 MS.gene049657:CDS 25.0%
!! TTGTGCAAGAAAGTATAAAT+TGG - chr3.2:71604587-71604606 None:intergenic 25.0%
!!! AAAAGATTGGTTTCTGATAT+TGG + chr3.2:71605251-71605270 MS.gene049657:CDS 25.0%
!!! AAATCAGGTATTTTTTCAAC+TGG - chr3.2:71605314-71605333 None:intergenic 25.0%
ACAGGAATATATGTTGAAGA+TGG + chr3.2:71604708-71604727 MS.gene049657:intron 30.0%
CACAGTTATTCTTGAAGATA+TGG - chr3.2:71605061-71605080 None:intergenic 30.0%
CGAAAGAACTTGTAAAGAAA+CGG - chr3.2:71604797-71604816 None:intergenic 30.0%
TCAGTATGATAGTTATGCAT+AGG - chr3.2:71604642-71604661 None:intergenic 30.0%
TTCAAGAAGATATAAAGCAC+GGG - chr3.2:71605021-71605040 None:intergenic 30.0%
TTGGGCTAAAAAATGTAGTT+TGG - chr3.2:71604943-71604962 None:intergenic 30.0%
TTTGCAGTTTATTGCAAAAC+AGG + chr3.2:71605086-71605105 MS.gene049657:CDS 30.0%
! AAAAATACCTGATTTGACCA+TGG + chr3.2:71605319-71605338 MS.gene049657:CDS 30.0%
AAGAAACGGTATAAAATCGC+TGG - chr3.2:71604783-71604802 None:intergenic 35.0%
ATCCATTTCACAATTGGATC+AGG + chr3.2:71604738-71604757 MS.gene049657:CDS 35.0%
ATGGTGATCCATTTCACAAT+TGG + chr3.2:71604732-71604751 MS.gene049657:CDS 35.0%
CGGCATTTCTGTAATATAAC+AGG + chr3.2:71604690-71604709 MS.gene049657:intron 35.0%
GAACTCTACCTCTTTCAATA+TGG + chr3.2:71604912-71604931 MS.gene049657:CDS 35.0%
TCAAGAAGATATAAAGCACG+GGG - chr3.2:71605020-71605039 None:intergenic 35.0%
TCATATTTGTGTCCTGCTAA+CGG + chr3.2:71604670-71604689 MS.gene049657:intron 35.0%
TGAAAGAAAGCAATCTAAGC+AGG - chr3.2:71604886-71604905 None:intergenic 35.0%
TGCTTAGATTGCTTTCTTTC+AGG + chr3.2:71604885-71604904 MS.gene049657:CDS 35.0%
TGCTTTCCAATAAGACTGAT+CGG + chr3.2:71604469-71604488 MS.gene049657:CDS 35.0%
TTAGATTGCTTTCTTTCAGG+AGG + chr3.2:71604888-71604907 MS.gene049657:CDS 35.0%
! ATCGGAAACATCTAAAACCT+GGG + chr3.2:71604487-71604506 MS.gene049657:CDS 35.0%
! ATGTTTCCGATCAGTCTTAT+TGG - chr3.2:71604478-71604497 None:intergenic 35.0%
! TTTGTGAACTCCAGTCTTTT+TGG + chr3.2:71605194-71605213 MS.gene049657:CDS 35.0%
!!! GGTCTTTTTTCAACCTCAAA+AGG + chr3.2:71605107-71605126 MS.gene049657:CDS 35.0%
ACATCTTTGGCATGGAATGT+TGG - chr3.2:71604823-71604842 None:intergenic 40.0%
ATTACAGAAATGCCGTTAGC+AGG - chr3.2:71604685-71604704 None:intergenic 40.0%
CAAGAGTAAATATGATCCCC+AGG - chr3.2:71604507-71604526 None:intergenic 40.0%
CTGGGGATCATATTTACTCT+TGG + chr3.2:71604505-71604524 MS.gene049657:CDS 40.0%
CTGTAGACTCTTCATCACTT+CGG + chr3.2:71605171-71605190 MS.gene049657:CDS 40.0%
GGGATCATATTTACTCTTGG+AGG + chr3.2:71604508-71604527 MS.gene049657:CDS 40.0%
GTCCTGATCCAATTGTGAAA+TGG - chr3.2:71604743-71604762 None:intergenic 40.0%
TCATATTTACTCTTGGAGGC+AGG + chr3.2:71604512-71604531 MS.gene049657:CDS 40.0%
TGGAGACACCATATTGAAAG+AGG - chr3.2:71604923-71604942 None:intergenic 40.0%
! ATACCTGATTTGACCATGGA+TGG + chr3.2:71605323-71605342 MS.gene049657:CDS 40.0%
! GATCGGAAACATCTAAAACC+TGG + chr3.2:71604486-71604505 MS.gene049657:CDS 40.0%
! TCGGAAACATCTAAAACCTG+GGG + chr3.2:71604488-71604507 MS.gene049657:CDS 40.0%
!! GAACTCCAGTCTTTTTGGTA+GGG + chr3.2:71605199-71605218 MS.gene049657:CDS 40.0%
!! TGAACTCCAGTCTTTTTGGT+AGG + chr3.2:71605198-71605217 MS.gene049657:CDS 40.0%
!!! CATTTTTTAGCCCAAGCTAG+AGG + chr3.2:71604948-71604967 MS.gene049657:CDS 40.0%
CAAAGCCCTACCAAAAAGAC+TGG - chr3.2:71605207-71605226 None:intergenic 45.0%
CTTCCATCCATGGTCAAATC+AGG - chr3.2:71605329-71605348 None:intergenic 45.0%
GAAGATATAAAGCACGGGGA+AGG - chr3.2:71605016-71605035 None:intergenic 45.0%
GATGACCATGATGTGCATAG+AGG - chr3.2:71604542-71604561 None:intergenic 45.0%
GCTTACCTCTATGCACATCA+TGG + chr3.2:71604534-71604553 MS.gene049657:CDS 45.0%
TGATGAAGAGTCTACAGTGC+TGG - chr3.2:71605168-71605187 None:intergenic 45.0%
!! CAGTCTTTTTGGTAGGGCTT+TGG + chr3.2:71605205-71605224 MS.gene049657:CDS 45.0%
!! TTTTTAGCCCAAGCTAGAGG+AGG + chr3.2:71604951-71604970 MS.gene049657:CDS 45.0%
AGTTGGATCCGAAGCAGCTA+GGG - chr3.2:71604985-71605004 None:intergenic 50.0%
CCATGCCAAAGATGTCGTGA+CGG + chr3.2:71604828-71604847 MS.gene049657:CDS 50.0%
TCAACCTCAAAAGGCTGCAG+TGG + chr3.2:71605116-71605135 MS.gene049657:CDS 50.0%
TGCTTCGGATCCAACTGAAG+AGG + chr3.2:71604989-71605008 MS.gene049657:CDS 50.0%
! TTGACCACTGCAGCCTTTTG+AGG - chr3.2:71605123-71605142 None:intergenic 50.0%
CACATGCACCCTAGCTGCTT+CGG + chr3.2:71604974-71604993 MS.gene049657:CDS 55.0%
CAGTTGGATCCGAAGCAGCT+AGG - chr3.2:71604986-71605005 None:intergenic 55.0%
CCAAAGATGTCGTGACGGTG+CGG + chr3.2:71604833-71604852 MS.gene049657:CDS 55.0%
CCGTCACGACATCTTTGGCA+TGG - chr3.2:71604831-71604850 None:intergenic 55.0%
GCTGGCAGCAGCTGAATAAG+AGG - chr3.2:71605150-71605169 None:intergenic 55.0%
GGTGCGGAAACAAGGACTCA+TGG + chr3.2:71604849-71604868 MS.gene049657:CDS 55.0%
TGCATGTGCCTCCTCTAGCT+TGG - chr3.2:71604962-71604981 None:intergenic 55.0%
! GCATGTGCCTCCTCTAGCTT+GGG - chr3.2:71604961-71604980 None:intergenic 55.0%
!! GGTAGGGCTTTGGTTTGCTG+TGG + chr3.2:71605215-71605234 MS.gene049657:CDS 55.0%
CCGCACCGTCACGACATCTT+TGG - chr3.2:71604836-71604855 None:intergenic 60.0%
CGGGGAAGGACCTCTTCAGT+TGG - chr3.2:71605002-71605021 None:intergenic 60.0%
GGATCAGGACAGCAAGCTGC+TGG + chr3.2:71604753-71604772 MS.gene049657:CDS 60.0%
GTCGTGACGGTGCGGAAACA+AGG + chr3.2:71604841-71604860 MS.gene049657:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 71604462 71605352 71604462 ID=MS.gene049657
chr3.2 mRNA 71604462 71605352 71604462 ID=MS.gene049657.t1;Parent=MS.gene049657
chr3.2 exon 71604462 71604555 71604462 ID=MS.gene049657.t1.exon1;Parent=MS.gene049657.t1
chr3.2 CDS 71604462 71604555 71604462 ID=cds.MS.gene049657.t1;Parent=MS.gene049657.t1
chr3.2 exon 71604712 71605352 71604712 ID=MS.gene049657.t1.exon2;Parent=MS.gene049657.t1
chr3.2 CDS 71604712 71605352 71604712 ID=cds.MS.gene049657.t1;Parent=MS.gene049657.t1
Gene Sequence

>MS.gene049657

ATGGGAGTGCTTTCCAATAAGACTGATCGGAAACATCTAAAACCTGGGGATCATATTTACTCTTGGAGGCAGGCTTACCTCTATGCACATCATGGTCATCTCTTTTCTCTTATATTTATCTGCCAATTTATACTTTCTTGCACAACTATGAACCATTTTCTTATGTATCAAACTTATCCTATGCATAACTATCATACTGATGTTAATGTCATATTTGTGTCCTGCTAACGGCATTTCTGTAATATAACAGGAATATATGTTGAAGATGGAATGGTGATCCATTTCACAATTGGATCAGGACAGCAAGCTGCTGGAACACCAGCGATTTTATACCGTTTCTTTACAAGTTCTTTCGATGCCAACATTCCATGCCAAAGATGTCGTGACGGTGCGGAAACAAGGACTCATGGTGTCTTCTCATCCTGCTTAGATTGCTTTCTTTCAGGAGGTGAACTCTACCTCTTTCAATATGGTGTCTCCAAACTACATTTTTTAGCCCAAGCTAGAGGAGGCACATGCACCCTAGCTGCTTCGGATCCAACTGAAGAGGTCCTTCCCCGTGCTTTATATCTTCTTGAAAATGGATTTGGTGACTACCATATCTTCAAGAATAACTGTGAAGATTTTGCAGTTTATTGCAAAACAGGTCTTTTTTCAACCTCAAAAGGCTGCAGTGGTCAAGCAGCCTCTTATTCAGCTGCTGCCAGCACTGTAGACTCTTCATCACTTCGGTTTGTGAACTCCAGTCTTTTTGGTAGGGCTTTGGTTTGCTGTGGAACTTACTGCATTAAAAGATTGGTTTCTGATATTGGATTTCGTTATGAAGTAATTAAAGATGTAACTAAAGTTCCAGTTGAAAAAATACCTGATTTGACCATGGATGGAAGCTAG

Protein sequence

>MS.gene049657.t1

MGVLSNKTDRKHLKPGDHIYSWRQAYLYAHHGIYVEDGMVIHFTIGSGQQAAGTPAILYRFFTSSFDANIPCQRCRDGAETRTHGVFSSCLDCFLSGGELYLFQYGVSKLHFLAQARGGTCTLAASDPTEEVLPRALYLLENGFGDYHIFKNNCEDFAVYCKTGLFSTSKGCSGQAASYSAAASTVDSSSLRFVNSSLFGRALVCCGTYCIKRLVSDIGFRYEVIKDVTKVPVEKIPDLTMDGS