Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07872.t1 | AAP47635.1 | 100 | 75 | 0 | 0 | 1 | 75 | 84 | 158 | 1.60E-37 | 164.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07872.t1 | Q9FNA9 | 89.3 | 75 | 8 | 0 | 1 | 75 | 75 | 149 | 1.9e-36 | 152.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07872.t1 | A0A072TNQ7 | 100.0 | 75 | 0 | 0 | 1 | 75 | 84 | 158 | 1.1e-37 | 164.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene07872 | 0.816639 | 4.98E-52 | -1.69E-46 |
| MS.gene049435 | MS.gene07872 | 0.809209 | 2.12E-50 | -1.69E-46 |
| MS.gene049585 | MS.gene07872 | 0.814523 | 1.48E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene07872 | 0.839068 | 1.98E-57 | -1.69E-46 |
| MS.gene050227 | MS.gene07872 | 0.821025 | 5.03E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene07872 | 0.820605 | 6.28E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene07872 | 0.82954 | 4.86E-55 | -1.69E-46 |
| MS.gene050680 | MS.gene07872 | 0.805643 | 1.21E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene07872 | 0.812028 | 5.21E-51 | -1.69E-46 |
| MS.gene051102 | MS.gene07872 | 0.804146 | 2.48E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene07872 | 0.809406 | 1.92E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene07872 | 0.811764 | 5.95E-51 | -1.69E-46 |
| MS.gene052860 | MS.gene07872 | 0.805683 | 1.19E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene07872 | 0.804537 | 2.06E-49 | -1.69E-46 |
| MS.gene053565 | MS.gene07872 | 0.808288 | 3.33E-50 | -1.69E-46 |
| MS.gene054379 | MS.gene07872 | 0.819435 | 1.16E-52 | -1.69E-46 |
| MS.gene05462 | MS.gene07872 | 0.808432 | 3.11E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene07872 | 0.805429 | 1.34E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene07872 | 0.834771 | 2.47E-56 | -1.69E-46 |
| MS.gene055227 | MS.gene07872 | 0.803407 | 3.54E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene07872 | 0.80688 | 6.64E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene07872 | 0.81111 | 8.25E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene07872 | 0.807153 | 5.81E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene07872 | 0.813287 | 2.76E-51 | -1.69E-46 |
| MS.gene057314 | MS.gene07872 | 0.800348 | 1.50E-48 | -1.69E-46 |
| MS.gene057750 | MS.gene07872 | 0.802467 | 5.53E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene07872 | 0.850463 | 1.67E-60 | -1.69E-46 |
| MS.gene058010 | MS.gene07872 | 0.821277 | 4.40E-53 | -1.69E-46 |
| MS.gene058548 | MS.gene07872 | 0.821717 | 3.48E-53 | -1.69E-46 |
| MS.gene058595 | MS.gene07872 | 0.821689 | 3.53E-53 | -1.69E-46 |
| MS.gene059128 | MS.gene07872 | 0.843432 | 1.41E-58 | -1.69E-46 |
| MS.gene059161 | MS.gene07872 | 0.805533 | 1.28E-49 | -1.69E-46 |
| MS.gene059713 | MS.gene07872 | 0.821014 | 5.06E-53 | -1.69E-46 |
| MS.gene060011 | MS.gene07872 | 0.83266 | 8.33E-56 | -1.69E-46 |
| MS.gene060167 | MS.gene07872 | 0.820973 | 5.17E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene07872 | 0.816786 | 4.62E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene07872 | 0.811686 | 6.18E-51 | -1.69E-46 |
| MS.gene060683 | MS.gene07872 | 0.829094 | 6.24E-55 | -1.69E-46 |
| MS.gene060685 | MS.gene07872 | 0.826199 | 3.09E-54 | -1.69E-46 |
| MS.gene060802 | MS.gene07872 | 0.808761 | 2.64E-50 | -1.69E-46 |
| MS.gene060918 | MS.gene07872 | 0.837632 | 4.64E-57 | -1.69E-46 |
| MS.gene060998 | MS.gene07872 | 0.832948 | 7.06E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07872.t1 | MTR_8g019570 | 100.000 | 75 | 0 | 0 | 1 | 75 | 84 | 158 | 3.39e-47 | 158 |
| MS.gene07872.t1 | MTR_4g056140 | 57.143 | 70 | 29 | 1 | 7 | 75 | 38 | 107 | 2.98e-24 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07872.t1 | AT5G13640 | 89.333 | 75 | 8 | 0 | 1 | 75 | 75 | 149 | 1.83e-42 | 145 |
| MS.gene07872.t1 | AT3G44830 | 63.793 | 58 | 21 | 0 | 18 | 75 | 75 | 132 | 3.47e-23 | 91.7 |
Find 0 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTGGAGAAGTTTATAAA+AGG | - | 56885:1799-1818 | MS.gene07872:CDS | 25.0% |
| ATTGTTTAGGAAGAGATTGT+GGG | - | 56885:1770-1789 | MS.gene07872:CDS | 30.0% | |
| TCTTTCTTCAATTTCAAACC+AGG | + | 56885:1660-1679 | None:intergenic | 30.0% | |
| ! | GGTTTGAAATTGAAGAAAGA+AGG | - | 56885:1660-1679 | MS.gene07872:CDS | 30.0% |
| GATTGTTTAGGAAGAGATTG+TGG | - | 56885:1769-1788 | MS.gene07872:CDS | 35.0% | |
| TTCTTCAATTTCAAACCAGG+TGG | + | 56885:1657-1676 | None:intergenic | 35.0% | |
| TTGTTTAGGAAGAGATTGTG+GGG | - | 56885:1771-1790 | MS.gene07872:CDS | 35.0% | |
| AATTTCAAACCAGGTGGATC+AGG | + | 56885:1651-1670 | None:intergenic | 40.0% | |
| ACATACTGAGGAAATGAAGC+TGG | + | 56885:1603-1622 | None:intergenic | 40.0% | |
| AGTGTGCTGAAGGATTGTTT+AGG | - | 56885:1757-1776 | MS.gene07872:CDS | 40.0% | |
| ATTTCCTCAGTATGTCACTG+AGG | - | 56885:1608-1627 | MS.gene07872:CDS | 40.0% | |
| TATGTCACTGAGGCTATAAC+TGG | - | 56885:1618-1637 | MS.gene07872:CDS | 40.0% | |
| !!! | ATCCAGTTGTTTTTGTTCCG+GGG | - | 56885:1697-1716 | MS.gene07872:CDS | 40.0% |
| !!! | CATCCAGTTGTTTTTGTTCC+GGG | - | 56885:1696-1715 | MS.gene07872:CDS | 40.0% |
| !!! | GCATCCAGTTGTTTTTGTTC+CGG | - | 56885:1695-1714 | MS.gene07872:CDS | 40.0% |
| ATAGCCTCAGTGACATACTG+AGG | + | 56885:1615-1634 | None:intergenic | 45.0% | |
| ATCCCCGGAACAAAAACAAC+TGG | + | 56885:1702-1721 | None:intergenic | 45.0% | |
| TTAGGAAGAGATTGTGGGGT+GGG | - | 56885:1775-1794 | MS.gene07872:CDS | 45.0% | |
| TTTAGGAAGAGATTGTGGGG+TGG | - | 56885:1774-1793 | MS.gene07872:CDS | 45.0% | |
| TTTGTTCCGGGGATTGTTAC+CGG | - | 56885:1708-1727 | MS.gene07872:CDS | 45.0% | |
| ! | TACCGGTGGACTTGAACTTT+GGG | - | 56885:1725-1744 | MS.gene07872:CDS | 45.0% |
| ! | TTACCGGTGGACTTGAACTT+TGG | - | 56885:1724-1743 | MS.gene07872:CDS | 45.0% |
| AAACCAGGTGGATCAGGCAT+AGG | + | 56885:1645-1664 | None:intergenic | 50.0% | |
| CTCCCAAAGTTCAAGTCCAC+CGG | + | 56885:1730-1749 | None:intergenic | 50.0% | |
| ! | AGATTGTGGGGTGGGACTTT+TGG | - | 56885:1783-1802 | MS.gene07872:CDS | 50.0% |
| ! | GGTGGACTTGAACTTTGGGA+GGG | - | 56885:1729-1748 | MS.gene07872:CDS | 50.0% |
| ! | GTGGACTTGAACTTTGGGAG+GGG | - | 56885:1730-1749 | MS.gene07872:CDS | 50.0% |
| AGTCCACCGGTAACAATCCC+CGG | + | 56885:1717-1736 | None:intergenic | 55.0% | |
| ! | CGGTGGACTTGAACTTTGGG+AGG | - | 56885:1728-1747 | MS.gene07872:CDS | 55.0% |
| GGTCCTATGCCTGATCCACC+TGG | - | 56885:1639-1658 | MS.gene07872:CDS | 60.0% | |
| GTTCCGGGGATTGTTACCGG+TGG | - | 56885:1711-1730 | MS.gene07872:CDS | 60.0% | |
| !! | GAGGGGCATCAGTGTGCTGA+AGG | - | 56885:1747-1766 | MS.gene07872:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 56885 | gene | 1597 | 1836 | 1597 | ID=MS.gene07872 |
| 56885 | mRNA | 1597 | 1836 | 1597 | ID=MS.gene07872.t1;Parent=MS.gene07872 |
| 56885 | exon | 1597 | 1836 | 1597 | ID=MS.gene07872.t1.exon1;Parent=MS.gene07872.t1 |
| 56885 | CDS | 1597 | 1836 | 1597 | ID=cds.MS.gene07872.t1;Parent=MS.gene07872.t1 |
| Gene Sequence |
| Protein sequence |
