Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20542.t1 | XP_003618434.1 | 96 | 176 | 7 | 0 | 1 | 176 | 1 | 176 | 2.70E-98 | 367.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20542.t1 | A0A1P8B554 | 43.3 | 180 | 94 | 4 | 3 | 175 | 5 | 183 | 1.0e-32 | 141.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20542.t1 | G7KK13 | 96.0 | 176 | 7 | 0 | 1 | 176 | 1 | 176 | 2.0e-98 | 367.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20542 | MS.gene015555 | PPI |
| MS.gene20542 | MS.gene021099 | PPI |
| MS.gene040126 | MS.gene20542 | PPI |
| MS.gene004055 | MS.gene20542 | PPI |
| MS.gene20542 | MS.gene014545 | PPI |
| MS.gene20542 | MS.gene24315 | PPI |
| MS.gene20542 | MS.gene26931 | PPI |
| MS.gene20542 | MS.gene69123 | PPI |
| MS.gene20542 | MS.gene27345 | PPI |
| MS.gene42360 | MS.gene20542 | PPI |
| MS.gene93762 | MS.gene20542 | PPI |
| MS.gene20542 | MS.gene41190 | PPI |
| MS.gene050380 | MS.gene20542 | PPI |
| MS.gene20542 | MS.gene019285 | PPI |
| MS.gene20542 | MS.gene046990 | PPI |
| MS.gene072805 | MS.gene20542 | PPI |
| MS.gene20542 | MS.gene61094 | PPI |
| MS.gene20542 | MS.gene062716 | PPI |
| MS.gene94553 | MS.gene20542 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20542.t1 | MTR_6g009480 | 96.023 | 176 | 7 | 0 | 1 | 176 | 1 | 176 | 2.80e-124 | 351 |
| MS.gene20542.t1 | MTR_6g079580 | 47.904 | 167 | 78 | 4 | 10 | 176 | 6 | 163 | 4.75e-46 | 151 |
| MS.gene20542.t1 | MTR_8g107140 | 47.917 | 144 | 69 | 3 | 33 | 176 | 31 | 168 | 3.65e-41 | 139 |
| MS.gene20542.t1 | MTR_3g068015 | 40.698 | 172 | 96 | 2 | 8 | 175 | 3 | 172 | 1.93e-39 | 134 |
| MS.gene20542.t1 | MTR_3g111620 | 43.293 | 164 | 88 | 4 | 13 | 176 | 12 | 170 | 3.70e-37 | 129 |
| MS.gene20542.t1 | MTR_4g073720 | 42.938 | 177 | 91 | 7 | 1 | 176 | 1 | 168 | 7.90e-37 | 130 |
| MS.gene20542.t1 | MTR_5g022350 | 44.242 | 165 | 80 | 7 | 14 | 176 | 17 | 171 | 9.09e-37 | 129 |
| MS.gene20542.t1 | MTR_1g025420 | 44.000 | 150 | 80 | 4 | 28 | 176 | 22 | 168 | 2.05e-35 | 124 |
| MS.gene20542.t1 | MTR_4g073730 | 44.379 | 169 | 89 | 5 | 10 | 176 | 5 | 170 | 3.81e-35 | 124 |
| MS.gene20542.t1 | MTR_1g025420 | 44.595 | 148 | 78 | 4 | 30 | 176 | 24 | 168 | 5.24e-35 | 125 |
| MS.gene20542.t1 | MTR_4g073730 | 44.379 | 169 | 89 | 5 | 10 | 176 | 5 | 170 | 5.81e-35 | 125 |
| MS.gene20542.t1 | MTR_8g075550 | 44.586 | 157 | 82 | 4 | 21 | 176 | 20 | 172 | 6.23e-35 | 124 |
| MS.gene20542.t1 | MTR_7g062610 | 40.936 | 171 | 96 | 3 | 6 | 176 | 2 | 167 | 1.32e-34 | 122 |
| MS.gene20542.t1 | MTR_1g062630 | 40.698 | 172 | 93 | 6 | 6 | 176 | 2 | 165 | 3.06e-34 | 121 |
| MS.gene20542.t1 | MTR_1g062590 | 39.535 | 172 | 95 | 5 | 6 | 176 | 2 | 165 | 3.86e-34 | 120 |
| MS.gene20542.t1 | MTR_3g114030 | 45.333 | 150 | 78 | 4 | 28 | 176 | 6 | 152 | 3.92e-34 | 120 |
| MS.gene20542.t1 | MTR_8g036215 | 44.099 | 161 | 84 | 4 | 17 | 176 | 7 | 162 | 4.19e-34 | 120 |
| MS.gene20542.t1 | MTR_8g075510 | 44.048 | 168 | 82 | 7 | 10 | 176 | 15 | 171 | 6.93e-34 | 120 |
| MS.gene20542.t1 | MTR_7g102380 | 39.412 | 170 | 94 | 6 | 8 | 176 | 3 | 164 | 9.73e-34 | 119 |
| MS.gene20542.t1 | MTR_1g062370 | 42.857 | 161 | 83 | 6 | 17 | 176 | 13 | 165 | 1.04e-33 | 119 |
| MS.gene20542.t1 | MTR_8g075510 | 44.048 | 168 | 82 | 7 | 10 | 176 | 15 | 171 | 1.23e-33 | 121 |
| MS.gene20542.t1 | MTR_1g021945 | 46.980 | 149 | 70 | 6 | 31 | 176 | 28 | 170 | 1.60e-33 | 119 |
| MS.gene20542.t1 | MTR_1g062350 | 42.857 | 161 | 83 | 6 | 17 | 176 | 13 | 165 | 2.28e-33 | 119 |
| MS.gene20542.t1 | MTR_5g022310 | 36.207 | 174 | 108 | 3 | 4 | 176 | 1 | 172 | 2.54e-33 | 119 |
| MS.gene20542.t1 | MTR_1g062340 | 40.994 | 161 | 86 | 6 | 17 | 176 | 13 | 165 | 3.53e-33 | 118 |
| MS.gene20542.t1 | MTR_5g059200 | 45.181 | 166 | 75 | 7 | 14 | 176 | 13 | 165 | 5.51e-33 | 119 |
| MS.gene20542.t1 | MTR_5g022310 | 36.207 | 174 | 108 | 3 | 4 | 176 | 1 | 172 | 5.60e-33 | 119 |
| MS.gene20542.t1 | MTR_2g069660 | 43.558 | 163 | 86 | 5 | 11 | 172 | 5 | 162 | 9.25e-33 | 117 |
| MS.gene20542.t1 | MTR_2g068030 | 43.243 | 148 | 79 | 4 | 31 | 176 | 25 | 169 | 9.55e-33 | 117 |
| MS.gene20542.t1 | MTR_1g062660 | 41.615 | 161 | 85 | 6 | 17 | 176 | 13 | 165 | 1.47e-32 | 117 |
| MS.gene20542.t1 | MTR_5g023850 | 39.506 | 162 | 93 | 4 | 18 | 176 | 15 | 174 | 2.49e-32 | 115 |
| MS.gene20542.t1 | MTR_8g037890 | 37.356 | 174 | 106 | 3 | 4 | 176 | 1 | 172 | 3.42e-32 | 117 |
| MS.gene20542.t1 | MTR_5g022350 | 44.371 | 151 | 72 | 7 | 14 | 162 | 82 | 222 | 6.11e-32 | 114 |
| MS.gene20542.t1 | MTR_2g067980 | 38.506 | 174 | 100 | 4 | 7 | 176 | 2 | 172 | 8.05e-31 | 117 |
| MS.gene20542.t1 | MTR_4g063630 | 39.506 | 162 | 92 | 4 | 18 | 177 | 17 | 174 | 3.62e-30 | 110 |
| MS.gene20542.t1 | MTR_2g068655 | 39.474 | 152 | 89 | 3 | 26 | 176 | 28 | 177 | 2.57e-29 | 110 |
| MS.gene20542.t1 | MTR_8g056820 | 37.572 | 173 | 104 | 4 | 6 | 176 | 2 | 172 | 3.08e-29 | 108 |
| MS.gene20542.t1 | MTR_2g067990 | 40.268 | 149 | 75 | 5 | 29 | 176 | 27 | 162 | 1.76e-25 | 102 |
| MS.gene20542.t1 | MTR_5g010635 | 40.397 | 151 | 74 | 6 | 27 | 176 | 22 | 157 | 1.88e-25 | 98.2 |
| MS.gene20542.t1 | MTR_8g096910 | 36.416 | 173 | 90 | 8 | 6 | 177 | 2 | 155 | 3.11e-24 | 93.6 |
| MS.gene20542.t1 | MTR_8g096920 | 39.073 | 151 | 75 | 7 | 23 | 172 | 13 | 147 | 1.07e-22 | 89.4 |
| MS.gene20542.t1 | MTR_8g096900 | 34.270 | 178 | 100 | 7 | 1 | 176 | 20 | 182 | 5.14e-21 | 87.0 |
| MS.gene20542.t1 | MTR_5g010640 | 33.333 | 177 | 99 | 8 | 1 | 176 | 1 | 159 | 1.33e-20 | 85.5 |
| MS.gene20542.t1 | MTR_1g062690 | 34.783 | 161 | 72 | 6 | 17 | 176 | 13 | 141 | 3.01e-20 | 84.3 |
| MS.gene20542.t1 | MTR_2g067970 | 39.640 | 111 | 59 | 5 | 70 | 176 | 1 | 107 | 2.67e-16 | 76.3 |
| MS.gene20542.t1 | MTR_7g076360 | 34.459 | 148 | 64 | 5 | 26 | 172 | 5 | 120 | 4.07e-15 | 69.7 |
| MS.gene20542.t1 | MTR_2g063150 | 33.088 | 136 | 84 | 4 | 41 | 175 | 46 | 175 | 1.54e-12 | 64.3 |
| MS.gene20542.t1 | MTR_8g089020 | 46.875 | 64 | 28 | 2 | 112 | 175 | 31 | 88 | 3.58e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20542.t1 | AT5G40020 | 64.327 | 171 | 58 | 2 | 6 | 176 | 5 | 172 | 7.76e-77 | 230 |
| MS.gene20542.t1 | AT4G24180 | 42.623 | 183 | 97 | 4 | 1 | 176 | 3 | 184 | 6.03e-41 | 139 |
| MS.gene20542.t1 | AT4G24180 | 42.541 | 181 | 95 | 4 | 1 | 176 | 3 | 179 | 1.33e-40 | 137 |
| MS.gene20542.t1 | AT5G24620 | 42.012 | 169 | 97 | 1 | 8 | 176 | 4 | 171 | 1.60e-40 | 141 |
| MS.gene20542.t1 | AT4G24180 | 42.623 | 183 | 97 | 4 | 1 | 176 | 3 | 184 | 1.79e-40 | 139 |
| MS.gene20542.t1 | AT5G24620 | 42.012 | 169 | 97 | 1 | 8 | 176 | 59 | 226 | 3.31e-40 | 142 |
| MS.gene20542.t1 | AT4G24180 | 42.541 | 181 | 95 | 4 | 1 | 176 | 3 | 179 | 4.09e-40 | 138 |
| MS.gene20542.t1 | AT4G24180 | 42.623 | 183 | 97 | 4 | 1 | 176 | 3 | 184 | 4.16e-40 | 138 |
| MS.gene20542.t1 | AT5G24620 | 42.012 | 169 | 97 | 1 | 8 | 176 | 80 | 247 | 4.36e-40 | 142 |
| MS.gene20542.t1 | AT4G24180 | 42.541 | 181 | 95 | 4 | 1 | 176 | 3 | 179 | 8.39e-40 | 137 |
| MS.gene20542.t1 | AT1G75050 | 42.771 | 166 | 87 | 6 | 11 | 176 | 9 | 166 | 6.27e-38 | 130 |
| MS.gene20542.t1 | AT1G18250 | 43.919 | 148 | 77 | 3 | 29 | 176 | 22 | 163 | 1.46e-37 | 129 |
| MS.gene20542.t1 | AT1G18250 | 43.919 | 148 | 77 | 3 | 29 | 176 | 21 | 162 | 1.48e-37 | 129 |
| MS.gene20542.t1 | AT4G38660 | 45.033 | 151 | 77 | 4 | 27 | 176 | 28 | 173 | 4.19e-37 | 131 |
| MS.gene20542.t1 | AT4G38660 | 44.516 | 155 | 80 | 4 | 23 | 176 | 2 | 151 | 4.56e-37 | 130 |
| MS.gene20542.t1 | AT1G75800 | 44.706 | 170 | 85 | 6 | 9 | 176 | 6 | 168 | 8.50e-37 | 130 |
| MS.gene20542.t1 | AT1G73620 | 42.105 | 152 | 82 | 3 | 25 | 176 | 38 | 183 | 3.97e-36 | 126 |
| MS.gene20542.t1 | AT2G28790 | 41.765 | 170 | 93 | 4 | 9 | 176 | 7 | 172 | 1.04e-35 | 125 |
| MS.gene20542.t1 | AT2G28790 | 46.528 | 144 | 71 | 4 | 35 | 176 | 58 | 197 | 1.25e-35 | 125 |
| MS.gene20542.t1 | AT1G75040 | 44.311 | 167 | 81 | 9 | 11 | 176 | 9 | 164 | 1.08e-34 | 122 |
| MS.gene20542.t1 | AT1G75030 | 40.476 | 168 | 89 | 7 | 10 | 176 | 9 | 166 | 4.40e-34 | 120 |
| MS.gene20542.t1 | AT4G38670 | 39.881 | 168 | 97 | 4 | 10 | 176 | 5 | 169 | 6.49e-34 | 121 |
| MS.gene20542.t1 | AT4G38670 | 39.881 | 168 | 97 | 4 | 10 | 176 | 5 | 169 | 6.49e-34 | 121 |
| MS.gene20542.t1 | AT2G17860 | 43.137 | 153 | 84 | 3 | 26 | 176 | 20 | 171 | 1.60e-33 | 119 |
| MS.gene20542.t1 | AT1G20030 | 44.595 | 148 | 78 | 4 | 30 | 176 | 4 | 148 | 7.50e-33 | 119 |
| MS.gene20542.t1 | AT1G20030 | 44.595 | 148 | 78 | 4 | 30 | 176 | 21 | 165 | 9.46e-33 | 119 |
| MS.gene20542.t1 | AT1G19320 | 42.045 | 176 | 89 | 7 | 6 | 176 | 5 | 172 | 1.76e-32 | 116 |
| MS.gene20542.t1 | AT5G02140 | 47.482 | 139 | 64 | 4 | 10 | 147 | 9 | 139 | 2.77e-32 | 116 |
| MS.gene20542.t1 | AT5G02140 | 47.482 | 139 | 64 | 4 | 10 | 147 | 9 | 139 | 3.98e-32 | 117 |
| MS.gene20542.t1 | AT4G38670 | 40.645 | 155 | 89 | 3 | 23 | 176 | 1 | 153 | 5.85e-32 | 115 |
| MS.gene20542.t1 | AT1G77700 | 46.207 | 145 | 67 | 5 | 31 | 175 | 63 | 196 | 2.05e-30 | 113 |
| MS.gene20542.t1 | AT1G77700 | 46.207 | 145 | 67 | 5 | 31 | 175 | 63 | 196 | 2.69e-30 | 112 |
| MS.gene20542.t1 | AT5G38280 | 36.905 | 168 | 100 | 4 | 10 | 176 | 9 | 171 | 3.71e-30 | 115 |
| MS.gene20542.t1 | AT5G38280 | 36.905 | 168 | 100 | 4 | 10 | 176 | 9 | 171 | 4.33e-30 | 115 |
| MS.gene20542.t1 | AT1G77700 | 46.207 | 145 | 67 | 5 | 31 | 175 | 91 | 224 | 4.94e-30 | 112 |
| MS.gene20542.t1 | AT4G36010 | 38.710 | 155 | 89 | 3 | 27 | 176 | 21 | 174 | 1.33e-28 | 108 |
| MS.gene20542.t1 | AT4G36010 | 38.710 | 155 | 89 | 3 | 27 | 176 | 21 | 174 | 1.33e-28 | 108 |
| MS.gene20542.t1 | AT4G11650 | 36.905 | 168 | 87 | 7 | 13 | 176 | 4 | 156 | 1.89e-24 | 95.9 |
| MS.gene20542.t1 | AT1G70250 | 40.136 | 147 | 77 | 6 | 30 | 175 | 146 | 282 | 3.88e-21 | 90.1 |
| MS.gene20542.t1 | AT4G18250 | 37.209 | 172 | 94 | 7 | 8 | 177 | 38 | 197 | 1.18e-20 | 88.6 |
| MS.gene20542.t1 | AT4G18253 | 33.333 | 168 | 99 | 5 | 9 | 175 | 6 | 161 | 1.42e-20 | 85.5 |
| MS.gene20542.t1 | AT4G36000 | 47.222 | 72 | 32 | 3 | 105 | 176 | 1 | 66 | 1.82e-11 | 60.5 |
| MS.gene20542.t1 | AT4G36000 | 47.222 | 72 | 32 | 3 | 105 | 176 | 1 | 66 | 1.82e-11 | 60.5 |
Find 48 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTCAGAATGCAGCTATT+AGG | 0.225225 | 6.3:+5289827 | None:intergenic |
| GGTCATGGAGTGGTAGAATT+TGG | 0.228734 | 6.3:+5290707 | MS.gene20542:CDS |
| AGACTTACATCATAGAAATT+TGG | 0.233214 | 6.3:-5290877 | None:intergenic |
| CCAGTGTTTGGTGCTGTTGC+TGG | 0.239326 | 6.3:-5290616 | None:intergenic |
| TGGAAACCATCTTGTGAAAC+TGG | 0.283626 | 6.3:+5290763 | MS.gene20542:CDS |
| ATCACACTTCAAGGTGATAA+AGG | 0.292993 | 6.3:+5290850 | MS.gene20542:CDS |
| GATTCAGAATGCAGCTATTA+GGG | 0.308672 | 6.3:+5289828 | None:intergenic |
| GAAGAAAATTAAAGGGGTTA+TGG | 0.311977 | 6.3:-5289852 | None:intergenic |
| GAAATTATGAAGAAAATTAA+AGG | 0.312086 | 6.3:-5289860 | None:intergenic |
| AATCCACCTTCAGCTATAAT+TGG | 0.318536 | 6.3:-5290643 | None:intergenic |
| ATAAATGTCCCTTTCCAATA+TGG | 0.329767 | 6.3:+5290593 | MS.gene20542:CDS |
| TGTTGATGGTTACAACATTC+CGG | 0.373965 | 6.3:+5290900 | MS.gene20542:CDS |
| TATGATGTAAGTCTTGTTGA+TGG | 0.380891 | 6.3:+5290886 | MS.gene20542:CDS |
| TTAGCTTGCAATGGACTCAT+TGG | 0.397789 | 6.3:+5290802 | MS.gene20542:CDS |
| CTTGTTGAAATCACACTTCA+AGG | 0.399140 | 6.3:+5290841 | MS.gene20542:CDS |
| GTCATGGAGTGGTAGAATTT+GGG | 0.407113 | 6.3:+5290708 | MS.gene20542:CDS |
| GGTGGATTCTACCTTCCTTC+AGG | 0.409270 | 6.3:+5290658 | MS.gene20542:CDS |
| AAATTATGAAGAAAATTAAA+GGG | 0.426233 | 6.3:-5289859 | None:intergenic |
| AATTCGAAGTGTAACATTCA+AGG | 0.437934 | 6.3:+5290952 | MS.gene20542:CDS |
| GGTGCTGTTGCTGGCCATAT+TGG | 0.448741 | 6.3:-5290607 | None:intergenic |
| CAATCCCCAGTTTCACAAGA+TGG | 0.451184 | 6.3:-5290769 | None:intergenic |
| ATAATTGGTTGGCCAGTGTT+TGG | 0.452074 | 6.3:-5290628 | None:intergenic |
| GGTAGAATTTGGGCTAGAAC+TGG | 0.458727 | 6.3:+5290718 | MS.gene20542:CDS |
| TCACACTTCAAGGTGATAAA+GGG | 0.463282 | 6.3:+5290851 | MS.gene20542:CDS |
| TAACATTCAAGGTTGTTGTA+AGG | 0.467243 | 6.3:+5290963 | MS.gene20542:CDS |
| TTAGCGACGACGACGGATAC+CGG | 0.481979 | 6.3:-5290919 | None:intergenic |
| CACCTTCAGCTATAATTGGT+TGG | 0.526943 | 6.3:-5290639 | None:intergenic |
| AAATTCTAGCACCATGGTCA+TGG | 0.538771 | 6.3:+5290692 | MS.gene20542:CDS |
| GGAAACCATCTTGTGAAACT+GGG | 0.562657 | 6.3:+5290764 | MS.gene20542:CDS |
| GTGATTTCAACAAGTGTAGC+TGG | 0.563029 | 6.3:-5290832 | None:intergenic |
| TCTTTGTTTGGCCTGAAGGA+AGG | 0.566171 | 6.3:-5290669 | None:intergenic |
| CCAGCAACAGCACCAAACAC+TGG | 0.580284 | 6.3:+5290616 | MS.gene20542:CDS |
| GTTGCTGGCCATATTGGAAA+GGG | 0.593909 | 6.3:-5290601 | None:intergenic |
| TTTGTGTCCTCGTGAGCTTG+AGG | 0.598513 | 6.3:+5290996 | MS.gene20542:CDS |
| GATGGAAGATTAGCTTGCAA+TGG | 0.600555 | 6.3:+5290793 | MS.gene20542:CDS |
| GGCCAACCAATTATAGCTGA+AGG | 0.602383 | 6.3:+5290637 | MS.gene20542:CDS |
| TGTTGCTGGCCATATTGGAA+AGG | 0.621427 | 6.3:-5290602 | None:intergenic |
| TGTGAAACTGGGGATTGTGA+TGG | 0.623588 | 6.3:+5290775 | MS.gene20542:CDS |
| CAAAGAAAATTCTAGCACCA+TGG | 0.637826 | 6.3:+5290686 | MS.gene20542:CDS |
| AATTATGAAGAAAATTAAAG+GGG | 0.640454 | 6.3:-5289858 | None:intergenic |
| TAATTTGTAGGGCTTGTCGA+AGG | 0.641451 | 6.3:+5290544 | MS.gene20542:intron |
| GAAACCATCTTGTGAAACTG+GGG | 0.649627 | 6.3:+5290765 | MS.gene20542:CDS |
| TTTAAAACCTCAAGCTCACG+AGG | 0.649763 | 6.3:-5291003 | None:intergenic |
| GTTCTTGTTAGCGACGACGA+CGG | 0.658078 | 6.3:-5290926 | None:intergenic |
| CAACCAATTATAGCTGAAGG+TGG | 0.659643 | 6.3:+5290640 | MS.gene20542:CDS |
| CTAGCACCATGGTCATGGAG+TGG | 0.668399 | 6.3:+5290697 | MS.gene20542:CDS |
| ATTCTACCACTCCATGACCA+TGG | 0.689426 | 6.3:-5290703 | None:intergenic |
| ATTTCAACAAGTGTAGCTGG+TGG | 0.757907 | 6.3:-5290829 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATATATATAT+GGG | + | chr6.3:5290244-5290263 | MS.gene20542:intron | 0.0% |
| !! | TATATATATATATATATATA+TGG | + | chr6.3:5290243-5290262 | MS.gene20542:intron | 0.0% |
| !! | AAATTATGAAGAAAATTAAA+GGG | - | chr6.3:5289862-5289881 | None:intergenic | 10.0% |
| !!! | TTTAATTTTCTTCATAATTT+CGG | + | chr6.3:5289862-5289881 | MS.gene20542:CDS | 10.0% |
| !! | AATTATGAAGAAAATTAAAG+GGG | - | chr6.3:5289861-5289880 | None:intergenic | 15.0% |
| !! | GAAATTATGAAGAAAATTAA+AGG | - | chr6.3:5289863-5289882 | None:intergenic | 15.0% |
| !! | TTAAATATAACTACGTATAT+AGG | - | chr6.3:5290479-5290498 | None:intergenic | 15.0% |
| !!! | ACTAAAACTAAATAATTTGT+AGG | + | chr6.3:5290532-5290551 | MS.gene20542:intron | 15.0% |
| !!! | CTAAAACTAAATAATTTGTA+GGG | + | chr6.3:5290533-5290552 | MS.gene20542:intron | 15.0% |
| !! | AAATCGCTTAAATGTTAAAT+CGG | - | chr6.3:5290116-5290135 | None:intergenic | 20.0% |
| !! | CTATAAATATAGATTTACCA+TGG | - | chr6.3:5289898-5289917 | None:intergenic | 20.0% |
| !!! | CTCAAATTTTGATTTAGATT+GGG | + | chr6.3:5290187-5290206 | MS.gene20542:intron | 20.0% |
| !!! | GTAATTTTGCTTCAAATAAT+TGG | + | chr6.3:5290743-5290762 | MS.gene20542:CDS | 20.0% |
| !!! | TCTCAAATTTTGATTTAGAT+TGG | + | chr6.3:5290186-5290205 | MS.gene20542:intron | 20.0% |
| ! | AGACTTACATCATAGAAATT+TGG | - | chr6.3:5290880-5290899 | None:intergenic | 25.0% |
| ! | TGAAATGGATAGATATATAG+TGG | - | chr6.3:5289924-5289943 | None:intergenic | 25.0% |
| !! | ATTTTCGCAATGAATCTTAT+TGG | + | chr6.3:5290050-5290069 | MS.gene20542:intron | 25.0% |
| !! | TAACATTTAAGCGATTTTAG+CGG | + | chr6.3:5290119-5290138 | MS.gene20542:intron | 25.0% |
| !!! | ATCTTGACAATTTTTGTTGT+AGG | + | chr6.3:5291073-5291092 | MS.gene20542:intron | 25.0% |
| !!! | CTTGAGGTTTTAAATTCTAA+TGG | + | chr6.3:5291012-5291031 | MS.gene20542:intron | 25.0% |
| AAATTCTAATGGAGAAGTTG+TGG | + | chr6.3:5291023-5291042 | MS.gene20542:intron | 30.0% | |
| AATTCGAAGTGTAACATTCA+AGG | + | chr6.3:5290952-5290971 | MS.gene20542:CDS | 30.0% | |
| ATAAATGTCCCTTTCCAATA+TGG | + | chr6.3:5290593-5290612 | MS.gene20542:CDS | 30.0% | |
| CACTATAGATGCATATGAAA+TGG | - | chr6.3:5289939-5289958 | None:intergenic | 30.0% | |
| GAAGAAAATTAAAGGGGTTA+TGG | - | chr6.3:5289855-5289874 | None:intergenic | 30.0% | |
| GACAATGCATAATATATGCA+AGG | + | chr6.3:5290399-5290418 | MS.gene20542:intron | 30.0% | |
| TAACATTCAAGGTTGTTGTA+AGG | + | chr6.3:5290963-5290982 | MS.gene20542:CDS | 30.0% | |
| TGCATAATATATGCAAGGTT+CGG | + | chr6.3:5290404-5290423 | MS.gene20542:intron | 30.0% | |
| TGGTTACTATTAGTAGTTAG+TGG | + | chr6.3:5290070-5290089 | MS.gene20542:intron | 30.0% | |
| ! | GTGCTAGAATTTTCTTTGTT+TGG | - | chr6.3:5290684-5290703 | None:intergenic | 30.0% |
| !! | TATGATGTAAGTCTTGTTGA+TGG | + | chr6.3:5290886-5290905 | MS.gene20542:CDS | 30.0% |
| AAGGACGTGTCATATATATG+TGG | - | chr6.3:5290350-5290369 | None:intergenic | 35.0% | |
| AATCCACCTTCAGCTATAAT+TGG | - | chr6.3:5290646-5290665 | None:intergenic | 35.0% | |
| AGGACGTGTCATATATATGT+GGG | - | chr6.3:5290349-5290368 | None:intergenic | 35.0% | |
| ATCACACTTCAAGGTGATAA+AGG | + | chr6.3:5290850-5290869 | MS.gene20542:CDS | 35.0% | |
| CAAAGAAAATTCTAGCACCA+TGG | + | chr6.3:5290686-5290705 | MS.gene20542:CDS | 35.0% | |
| CATAATATATGCAAGGTTCG+GGG | + | chr6.3:5290406-5290425 | MS.gene20542:intron | 35.0% | |
| CTTGTTGAAATCACACTTCA+AGG | + | chr6.3:5290841-5290860 | MS.gene20542:CDS | 35.0% | |
| GCATAATATATGCAAGGTTC+GGG | + | chr6.3:5290405-5290424 | MS.gene20542:intron | 35.0% | |
| TCACACTTCAAGGTGATAAA+GGG | + | chr6.3:5290851-5290870 | MS.gene20542:CDS | 35.0% | |
| ! | ATTTCGGCTTACATTTTCCA+TGG | + | chr6.3:5289878-5289897 | MS.gene20542:CDS | 35.0% |
| !! | ATTTTCTTTGTTTGGCCTGA+AGG | - | chr6.3:5290676-5290695 | None:intergenic | 35.0% |
| !! | TGTTGATGGTTACAACATTC+CGG | + | chr6.3:5290900-5290919 | MS.gene20542:CDS | 35.0% |
| !!! | AATGACGTGCCTTTTTTTTG+TGG | - | chr6.3:5290447-5290466 | None:intergenic | 35.0% |
| AAATTCTAGCACCATGGTCA+TGG | + | chr6.3:5290692-5290711 | MS.gene20542:CDS | 40.0% | |
| ATTTCAACAAGTGTAGCTGG+TGG | - | chr6.3:5290832-5290851 | None:intergenic | 40.0% | |
| CAACCAATTATAGCTGAAGG+TGG | + | chr6.3:5290640-5290659 | MS.gene20542:CDS | 40.0% | |
| CACCTTCAGCTATAATTGGT+TGG | - | chr6.3:5290642-5290661 | None:intergenic | 40.0% | |
| GAAACCATCTTGTGAAACTG+GGG | + | chr6.3:5290765-5290784 | MS.gene20542:CDS | 40.0% | |
| GAGTTAAGCTTACGAGAACA+AGG | - | chr6.3:5290369-5290388 | None:intergenic | 40.0% | |
| GGAAACCATCTTGTGAAACT+GGG | + | chr6.3:5290764-5290783 | MS.gene20542:CDS | 40.0% | |
| GTCATGGAGTGGTAGAATTT+GGG | + | chr6.3:5290708-5290727 | MS.gene20542:CDS | 40.0% | |
| GTGATTTCAACAAGTGTAGC+TGG | - | chr6.3:5290835-5290854 | None:intergenic | 40.0% | |
| TCTCGTAAGCTTAACTCAAG+TGG | + | chr6.3:5290372-5290391 | MS.gene20542:intron | 40.0% | |
| TGGAAACCATCTTGTGAAAC+TGG | + | chr6.3:5290763-5290782 | MS.gene20542:CDS | 40.0% | |
| ! | ATAATTGGTTGGCCAGTGTT+TGG | - | chr6.3:5290631-5290650 | None:intergenic | 40.0% |
| ! | TAATTTGTAGGGCTTGTCGA+AGG | + | chr6.3:5290544-5290563 | MS.gene20542:intron | 40.0% |
| ! | TTAGTAGTTAGTGGACGCTA+AGG | + | chr6.3:5290079-5290098 | MS.gene20542:intron | 40.0% |
| ! | TTTAAAACCTCAAGCTCACG+AGG | - | chr6.3:5291006-5291025 | None:intergenic | 40.0% |
| !! | GATGGAAGATTAGCTTGCAA+TGG | + | chr6.3:5290793-5290812 | MS.gene20542:CDS | 40.0% |
| !! | TTAGCTTGCAATGGACTCAT+TGG | + | chr6.3:5290802-5290821 | MS.gene20542:CDS | 40.0% |
| AAGGTTCGGGGTTTAAACCT+CGG | + | chr6.3:5290418-5290437 | MS.gene20542:intron | 45.0% | |
| AGTGGACGCTAAGGTTAATG+CGG | + | chr6.3:5290088-5290107 | MS.gene20542:intron | 45.0% | |
| ATTCTACCACTCCATGACCA+TGG | - | chr6.3:5290706-5290725 | None:intergenic | 45.0% | |
| CAATCCCCAGTTTCACAAGA+TGG | - | chr6.3:5290772-5290791 | None:intergenic | 45.0% | |
| CCTCGGTCACCACAAAAAAA+AGG | + | chr6.3:5290435-5290454 | MS.gene20542:intron | 45.0% | |
| GGCCAACCAATTATAGCTGA+AGG | + | chr6.3:5290637-5290656 | MS.gene20542:CDS | 45.0% | |
| GGTCATGGAGTGGTAGAATT+TGG | + | chr6.3:5290707-5290726 | MS.gene20542:CDS | 45.0% | |
| GTTGCTGGCCATATTGGAAA+GGG | - | chr6.3:5290604-5290623 | None:intergenic | 45.0% | |
| TGTGAAACTGGGGATTGTGA+TGG | + | chr6.3:5290775-5290794 | MS.gene20542:CDS | 45.0% | |
| TGTTGCTGGCCATATTGGAA+AGG | - | chr6.3:5290605-5290624 | None:intergenic | 45.0% | |
| ! | GGTAGAATTTGGGCTAGAAC+TGG | + | chr6.3:5290718-5290737 | MS.gene20542:CDS | 45.0% |
| ! | TCTTTGTTTGGCCTGAAGGA+AGG | - | chr6.3:5290672-5290691 | None:intergenic | 45.0% |
| !! | GGCTTGCAAAAGTGCTTGTT+TGG | + | chr6.3:5291044-5291063 | MS.gene20542:intron | 45.0% |
| !!! | CCTTTTTTTTGTGGTGACCG+AGG | - | chr6.3:5290438-5290457 | None:intergenic | 45.0% |
| !! | TATATATATATATATATATG+GGG | + | chr6.3:5290309-5290328 | MS.gene20542:intron | 5.0% |
| GTTCTTGTTAGCGACGACGA+CGG | - | chr6.3:5290929-5290948 | None:intergenic | 50.0% | |
| TTTGTGTCCTCGTGAGCTTG+AGG | + | chr6.3:5290996-5291015 | MS.gene20542:CDS | 50.0% | |
| ! | GGTGGATTCTACCTTCCTTC+AGG | + | chr6.3:5290658-5290677 | MS.gene20542:CDS | 50.0% |
| CCAGCAACAGCACCAAACAC+TGG | + | chr6.3:5290616-5290635 | MS.gene20542:CDS | 55.0% | |
| CTAGCACCATGGTCATGGAG+TGG | + | chr6.3:5290697-5290716 | MS.gene20542:CDS | 55.0% | |
| TTAGCGACGACGACGGATAC+CGG | - | chr6.3:5290922-5290941 | None:intergenic | 55.0% | |
| !! | CCAGTGTTTGGTGCTGTTGC+TGG | - | chr6.3:5290619-5290638 | None:intergenic | 55.0% |
| !! | GGTGCTGTTGCTGGCCATAT+TGG | - | chr6.3:5290610-5290629 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 5289836 | 5291100 | 5289836 | ID=MS.gene20542 |
| chr6.3 | mRNA | 5289836 | 5291100 | 5289836 | ID=MS.gene20542.t1;Parent=MS.gene20542 |
| chr6.3 | exon | 5289836 | 5289899 | 5289836 | ID=MS.gene20542.t1.exon1;Parent=MS.gene20542.t1 |
| chr6.3 | CDS | 5289836 | 5289899 | 5289836 | ID=cds.MS.gene20542.t1;Parent=MS.gene20542.t1 |
| chr6.3 | exon | 5290554 | 5291017 | 5290554 | ID=MS.gene20542.t1.exon2;Parent=MS.gene20542.t1 |
| chr6.3 | CDS | 5290554 | 5291017 | 5290554 | ID=cds.MS.gene20542.t1;Parent=MS.gene20542.t1 |
| chr6.3 | exon | 5291095 | 5291100 | 5291095 | ID=MS.gene20542.t1.exon3;Parent=MS.gene20542.t1 |
| chr6.3 | CDS | 5291095 | 5291100 | 5291095 | ID=cds.MS.gene20542.t1;Parent=MS.gene20542.t1 |
| Gene Sequence |
| Protein sequence |
