AlfalfaGEDB | MS.gene94553

M. sativa cultivar XinJiangDaYe / MS.gene94553

KEGG: -
GO: -
PFAM: PF00314.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94553.t1	XP_003631101.1	97.6	250	5	1	1	250	1	249	6.10E-144	520

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94553.t1	P50699	75.4	236	58	0	14	249	7	242	4.9e-110	398.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94553.t1	G7LDC8	97.6	250	5	1	1	250	1	249	4.4e-144	520.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049298	MS.gene94553	0.851379	9.22E-61	-1.69E-46
MS.gene050707	MS.gene94553	0.83556	1.56E-56	-1.69E-46
MS.gene050710	MS.gene94553	0.833617	4.81E-56	-1.69E-46
MS.gene051914	MS.gene94553	0.831067	2.06E-55	-1.69E-46
MS.gene052209	MS.gene94553	0.801778	7.67E-49	-1.69E-46
MS.gene052308	MS.gene94553	0.841931	3.52E-58	-1.69E-46
MS.gene053108	MS.gene94553	0.806771	7.00E-50	-1.69E-46
MS.gene054551	MS.gene94553	0.802886	4.53E-49	-1.69E-46
MS.gene054593	MS.gene94553	0.81414	1.79E-51	-1.69E-46
MS.gene054662	MS.gene94553	0.824029	1.01E-53	-1.69E-46
MS.gene054850	MS.gene94553	0.856646	2.80E-62	-1.69E-46
MS.gene054941	MS.gene94553	0.83139	1.72E-55	-1.69E-46
MS.gene055308	MS.gene94553	0.840545	8.15E-58	-1.69E-46
MS.gene055578	MS.gene94553	0.851924	6.47E-61	-1.69E-46
MS.gene056041	MS.gene94553	0.815155	1.07E-51	-1.69E-46
MS.gene05640	MS.gene94553	0.834466	2.95E-56	-1.69E-46
MS.gene05652	MS.gene94553	0.889064	3.38E-73	-1.69E-46
MS.gene057101	MS.gene94553	0.805917	1.06E-49	-1.69E-46
MS.gene057669	MS.gene94553	0.879844	8.94E-70	-1.69E-46
MS.gene058128	MS.gene94553	0.904287	1.44E-79	-1.69E-46
MS.gene058789	MS.gene94553	0.822819	1.93E-53	-1.69E-46
MS.gene058853	MS.gene94553	0.868212	7.72E-66	-1.69E-46
MS.gene05926	MS.gene94553	0.830594	2.69E-55	-1.69E-46
MS.gene059616	MS.gene94553	0.877646	5.32E-69	-1.69E-46
MS.gene059905	MS.gene94553	0.80925	2.08E-50	-1.69E-46
MS.gene06093	MS.gene94553	0.813743	2.19E-51	-1.69E-46
MS.gene061089	MS.gene94553	0.806912	6.53E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene94553	MS.gene61094	PPI
MS.gene94553	MS.gene046990	PPI
MS.gene040126	MS.gene94553	PPI
MS.gene94553	MS.gene92395	PPI
MS.gene94553	MS.gene92393	PPI
MS.gene94553	MS.gene42714	PPI
MS.gene94553	MS.gene42360	PPI
MS.gene94553	MS.gene062716	PPI
MS.gene056186	MS.gene94553	PPI
MS.gene94553	MS.gene68072	PPI
MS.gene94553	MS.gene050380	PPI
MS.gene94553	MS.gene004055	PPI
MS.gene94553	MS.gene45764	PPI
MS.gene94553	MS.gene015555	PPI
MS.gene94553	MS.gene26931	PPI
MS.gene94553	MS.gene93762	PPI
MS.gene94553	MS.gene021099	PPI
MS.gene94553	MS.gene59476	PPI
MS.gene94553	MS.gene016652	PPI
MS.gene94553	MS.gene056184	PPI
MS.gene94553	MS.gene69123	PPI
MS.gene94553	MS.gene019285	PPI
MS.gene94553	MS.gene014545	PPI
MS.gene94553	MS.gene41190	PPI
MS.gene94553	MS.gene64778	PPI
MS.gene94553	MS.gene072805	PPI
MS.gene94553	MS.gene24315	PPI
MS.gene94553	MS.gene56731	PPI
MS.gene94553	MS.gene27345	PPI
MS.gene94553	MS.gene92397	PPI
MS.gene94553	MS.gene010631	PPI
MS.gene94553	MS.gene20542	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94553.t1	MTR_8g107140	97.600	250	5	1	1	250	1	249	0.0	498
MS.gene94553.t1	MTR_6g079580	69.328	238	72	1	9	246	4	240	4.70e-129	365
MS.gene94553.t1	MTR_8g036215	59.091	220	88	2	27	245	19	237	7.80e-92	270
MS.gene94553.t1	MTR_3g111620	54.626	227	98	3	23	246	22	246	6.70e-88	261
MS.gene94553.t1	MTR_8g075550	54.936	233	95	4	27	250	27	258	1.62e-84	254
MS.gene94553.t1	MTR_4g073720	51.639	244	102	5	4	245	10	239	3.13e-82	250
MS.gene94553.t1	MTR_1g025420	52.893	242	109	4	9	245	4	245	5.75e-82	248
MS.gene94553.t1	MTR_1g025420	52.893	242	109	4	9	245	4	245	7.64e-82	246
MS.gene94553.t1	MTR_7g062610	50.000	236	112	3	14	246	10	242	1.56e-81	244
MS.gene94553.t1	MTR_1g021945	54.585	229	97	3	23	247	21	246	2.69e-81	244
MS.gene94553.t1	MTR_3g068015	49.597	248	115	4	7	246	3	248	3.17e-81	244
MS.gene94553.t1	MTR_8g075510	53.750	240	99	6	10	246	14	244	1.56e-80	242
MS.gene94553.t1	MTR_5g022350	54.709	223	91	4	26	246	29	243	6.96e-80	243
MS.gene94553.t1	MTR_8g075510	53.556	239	99	6	10	245	14	243	1.55e-79	242
MS.gene94553.t1	MTR_8g037890	49.801	251	117	5	1	246	1	247	1.37e-78	239
MS.gene94553.t1	MTR_5g059200	53.097	226	99	4	25	250	26	244	2.50e-77	235
MS.gene94553.t1	MTR_5g022310	50.198	253	117	6	1	246	1	251	1.17e-76	233
MS.gene94553.t1	MTR_5g022310	50.198	253	117	6	1	246	1	251	2.03e-76	233
MS.gene94553.t1	MTR_7g102380	52.232	224	101	5	28	247	21	242	7.89e-75	228
MS.gene94553.t1	MTR_3g114030	50.218	229	109	4	27	250	6	234	2.06e-73	224
MS.gene94553.t1	MTR_1g062590	47.325	243	122	5	8	246	2	242	5.52e-73	223
MS.gene94553.t1	MTR_8g056820	48.374	246	117	5	6	246	7	247	8.14e-73	223
MS.gene94553.t1	MTR_6g009480	44.269	253	133	6	1	247	3	253	4.40e-71	219
MS.gene94553.t1	MTR_1g062370	48.117	239	118	5	12	246	6	242	1.34e-70	217
MS.gene94553.t1	MTR_4g073730	49.794	243	115	6	9	246	8	248	7.80e-70	216
MS.gene94553.t1	MTR_1g062350	47.699	239	119	5	12	246	6	242	8.10e-70	215
MS.gene94553.t1	MTR_1g062340	47.899	238	118	5	13	246	7	242	8.10e-70	215
MS.gene94553.t1	MTR_2g069660	50.442	226	107	4	25	246	19	243	1.01e-69	214
MS.gene94553.t1	MTR_4g073730	49.794	243	115	6	9	246	8	248	1.57e-69	217
MS.gene94553.t1	MTR_1g062660	48.458	227	111	5	26	248	20	244	1.18e-68	212
MS.gene94553.t1	MTR_1g062630	45.528	246	128	5	8	249	2	245	3.32e-68	211
MS.gene94553.t1	MTR_2g068030	46.725	229	115	5	22	245	17	243	2.23e-67	209
MS.gene94553.t1	MTR_2g067980	46.939	245	118	6	9	246	8	247	6.80e-65	218
MS.gene94553.t1	MTR_4g063630	44.538	238	121	6	14	245	19	251	2.41e-61	193
MS.gene94553.t1	MTR_2g068655	44.541	229	120	5	27	250	30	256	7.99e-61	194
MS.gene94553.t1	MTR_1g062690	43.038	237	109	5	12	246	6	218	1.42e-57	182
MS.gene94553.t1	MTR_5g010640	38.340	253	128	8	4	246	2	236	1.74e-48	160
MS.gene94553.t1	MTR_5g010635	40.889	225	110	7	25	246	21	225	2.89e-48	159
MS.gene94553.t1	MTR_2g067970	45.902	183	89	7	71	246	3	182	4.93e-47	167
MS.gene94553.t1	MTR_8g096900	36.546	249	126	11	3	246	27	248	1.30e-43	148
MS.gene94553.t1	MTR_8g089020	48.936	141	69	2	101	241	22	159	4.73e-43	144
MS.gene94553.t1	MTR_5g022350	52.239	134	59	3	24	155	92	222	1.02e-40	139
MS.gene94553.t1	MTR_2g067990	37.500	224	109	9	27	246	26	222	6.10e-40	147
MS.gene94553.t1	MTR_2g063150	33.659	205	128	5	28	228	34	234	6.50e-36	128
MS.gene94553.t1	MTR_5g023850	34.400	250	119	10	7	247	4	217	2.58e-35	125
MS.gene94553.t1	MTR_1g062390	31.225	253	152	7	1	246	1	238	1.00e-30	114
MS.gene94553.t1	MTR_7g076360	33.649	211	77	4	27	236	7	155	1.59e-28	106
MS.gene94553.t1	MTR_8g096910	40.559	143	70	5	4	143	2	132	3.87e-28	105
MS.gene94553.t1	MTR_8g096920	42.623	122	59	4	25	143	16	129	8.91e-25	97.1
MS.gene94553.t1	MTR_3g081550	42.553	94	51	2	112	202	16	109	1.73e-15	70.9
MS.gene94553.t1	MTR_1g062640	31.507	146	90	5	25	167	10	148	3.37e-14	68.6
MS.gene94553.t1	MTR_5g068670	48.750	80	37	2	65	142	12	89	4.14e-14	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94553.t1	AT1G18250	77.974	227	50	0	24	250	18	244	6.33e-135	380
MS.gene94553.t1	AT1G18250	77.434	226	51	0	25	250	18	243	4.34e-134	378
MS.gene94553.t1	AT1G73620	73.894	226	59	0	25	250	39	264	1.27e-129	367
MS.gene94553.t1	AT1G75050	55.462	238	102	3	14	249	11	246	5.82e-87	258
MS.gene94553.t1	AT1G75030	55.947	227	95	3	26	249	22	246	2.93e-85	254
MS.gene94553.t1	AT1G75040	55.833	240	95	6	9	246	9	239	1.41e-82	247
MS.gene94553.t1	AT1G19320	54.321	243	100	5	9	245	9	246	2.02e-82	247
MS.gene94553.t1	AT4G38660	51.304	230	105	3	21	248	1	225	6.66e-81	246
MS.gene94553.t1	AT4G38660	50.000	246	112	4	3	246	9	245	1.34e-80	246
MS.gene94553.t1	AT1G77700	53.712	229	98	5	23	250	55	276	1.10e-79	242
MS.gene94553.t1	AT1G77700	54.911	224	93	5	23	245	55	271	2.09e-79	242
MS.gene94553.t1	AT1G77700	53.680	231	99	5	16	245	76	299	2.38e-79	243
MS.gene94553.t1	AT1G75800	52.917	240	106	5	14	246	9	248	1.36e-77	238
MS.gene94553.t1	AT1G20030	54.667	225	95	5	29	246	4	228	1.71e-76	234
MS.gene94553.t1	AT1G20030	54.667	225	95	5	29	246	21	245	2.81e-76	234
MS.gene94553.t1	AT4G24180	48.438	256	111	6	5	250	16	260	2.90e-75	229
MS.gene94553.t1	AT4G24180	50.424	236	102	5	25	250	25	255	9.64e-75	228
MS.gene94553.t1	AT4G24180	48.810	252	108	6	5	246	16	256	1.11e-74	229
MS.gene94553.t1	AT5G02140	47.280	239	120	4	12	246	5	241	2.86e-74	228
MS.gene94553.t1	AT4G24180	50.862	232	99	5	25	246	25	251	4.76e-74	228
MS.gene94553.t1	AT5G02140	47.280	239	120	4	12	246	5	241	5.09e-74	225
MS.gene94553.t1	AT5G40020	46.311	244	125	3	10	248	8	250	7.53e-74	226
MS.gene94553.t1	AT2G17860	47.755	245	120	5	14	250	9	253	7.72e-74	225
MS.gene94553.t1	AT4G36010	51.515	231	101	5	26	246	21	250	7.01e-73	224
MS.gene94553.t1	AT4G36010	51.515	231	101	5	26	246	21	250	7.01e-73	224
MS.gene94553.t1	AT5G24620	46.332	259	119	5	1	250	9	256	6.23e-71	223
MS.gene94553.t1	AT5G24620	46.332	259	119	5	1	250	64	311	1.15e-70	224
MS.gene94553.t1	AT5G24620	46.332	259	119	5	1	250	85	332	1.44e-70	224
MS.gene94553.t1	AT4G38670	48.387	248	115	6	5	246	7	247	3.69e-69	214
MS.gene94553.t1	AT4G38670	48.387	248	115	6	5	246	7	247	3.69e-69	214
MS.gene94553.t1	AT4G38670	51.339	224	103	5	29	246	8	231	5.95e-68	211
MS.gene94553.t1	AT4G24180	47.257	237	104	6	5	231	16	241	5.87e-66	207
MS.gene94553.t1	AT4G24180	49.309	217	95	5	25	231	25	236	2.66e-65	206
MS.gene94553.t1	AT2G28790	44.538	238	123	6	14	245	39	273	4.52e-64	201
MS.gene94553.t1	AT2G28790	44.538	238	123	6	14	245	14	248	6.41e-64	200
MS.gene94553.t1	AT5G38280	44.167	240	127	6	14	246	11	250	3.31e-60	201
MS.gene94553.t1	AT5G38280	44.167	240	127	6	14	246	11	250	4.15e-60	201
MS.gene94553.t1	AT4G11650	40.637	251	122	7	1	249	1	226	2.73e-57	183
MS.gene94553.t1	AT4G36000	58.503	147	51	3	103	247	1	139	1.61e-54	174
MS.gene94553.t1	AT4G36000	58.503	147	51	3	103	247	1	139	1.61e-54	174
MS.gene94553.t1	AT4G18253	39.076	238	137	4	14	249	10	241	1.88e-51	168
MS.gene94553.t1	AT1G70250	38.767	227	125	8	29	246	146	367	8.11e-43	155
MS.gene94553.t1	AT4G18250	36.864	236	140	6	14	246	43	272	1.10e-38	142
MS.gene94553.t1	AT5G24620	52.800	125	53	3	132	250	15	139	3.50e-35	127
MS.gene94553.t1	AT2G24810	58.442	77	32	0	169	245	116	192	1.64e-24	97.1

Find 95 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAATGGTCAGGCCGTTTC+TGG	0.068116	4.1:+383960	MS.gene94553:CDS
CCGGCACCGGGTTGGATTGC+TGG	0.183097	4.1:-383869	None:intergenic
TGAATAAGCAGTGGGCTTGC+AGG	0.307584	4.1:-384356	None:intergenic
GACCTCACCTGCAACCCTAC+TGG	0.350758	4.1:-384238	None:intergenic
ATTACACCTTTCAAGGGATC+TGG	0.363950	4.1:+384169	MS.gene94553:CDS
ACACTCGCTGAGTTCACATT+TGG	0.368237	4.1:+384088	MS.gene94553:CDS
CGGTGCCGGAAAACCCATTC+TGG	0.376903	4.1:+383883	MS.gene94553:CDS
GATTTCCATTACACCTTTCA+AGG	0.380242	4.1:+384162	MS.gene94553:CDS
GAAGCCGCCGCGGGCCAGAA+TGG	0.391505	4.1:-383897	None:intergenic
ACAATGGTCAGGCCGTTTCT+GGG	0.391880	4.1:+383961	MS.gene94553:CDS
TGAAGGGAATAAGCTTTGTT+TGG	0.395281	4.1:-383929	None:intergenic
TAAGCATAAGAGTAAGCCTT+TGG	0.402209	4.1:-384400	None:intergenic
GGTTGGATTGCTGGCCACAC+TGG	0.402359	4.1:-383860	None:intergenic
CACTCGCTGAGTTCACATTT+GGG	0.419380	4.1:+384089	MS.gene94553:CDS
AAGCCGCCGCGGGCCAGAAT+GGG	0.420312	4.1:-383896	None:intergenic
GATGTGCCCAGTAGGGTTGC+AGG	0.421685	4.1:+384231	MS.gene94553:CDS
CGCAGTCTCCGGTGGCGCAA+TGG	0.424632	4.1:-384021	None:intergenic
TTGAAAGGTGTAATGGAAAT+CGG	0.429153	4.1:-384160	None:intergenic
CAGTGGGCTTGCAGGTCTGT+GGG	0.433412	4.1:-384348	None:intergenic
ATTGGTGCAAGTAGCAATAC+TGG	0.439699	4.1:-384434	None:intergenic
GGGAATAAGCTTTGTTTGGT+GGG	0.442251	4.1:-383925	None:intergenic
ACCTCACCTGCAACCCTACT+GGG	0.443228	4.1:-384237	None:intergenic
GGCCACGGCCATTGCGCCAC+CGG	0.444546	4.1:+384013	MS.gene94553:CDS
CTCCGGTGGCGCAATGGCCG+TGG	0.450871	4.1:-384015	None:intergenic
ACCATTGTGGTGGGACTTGA+AGG	0.456165	4.1:-383946	None:intergenic
GTTGGATTGCTGGCCACACT+GGG	0.457650	4.1:-383859	None:intergenic
GGATTGCTGGCCACACTGGG+TGG	0.462751	4.1:-383856	None:intergenic
GCAGTGGGCTTGCAGGTCTG+TGG	0.469723	4.1:-384349	None:intergenic
TACCTGGAGGGTATGCGATT+AGG	0.471659	4.1:-383652	None:intergenic
GGCTGCACCTTCGACGCATC+AGG	0.474004	4.1:+383992	MS.gene94553:CDS
GAAGAAGAGAGCGGTGAAGA+TGG	0.478141	4.1:-383621	None:intergenic
AGATCCCTTGAAAGGTGTAA+TGG	0.479072	4.1:-384167	None:intergenic
GAGAGATCCGCCGCAGTCTC+CGG	0.481610	4.1:-384032	None:intergenic
AAGCATAAGAGTAAGCCTTT+GGG	0.484229	4.1:-384399	None:intergenic
GGCCGTTTCTGGGGCCGTCA+CGG	0.495582	4.1:+383971	MS.gene94553:CDS
AGCCGTGACGGCCCCAGAAA+CGG	0.498382	4.1:-383973	None:intergenic
TATTGCTGCACTGGTAACTT+TGG	0.498988	4.1:+384322	MS.gene94553:CDS
CCAGCAATCCAACCCGGTGC+CGG	0.501469	4.1:+383869	MS.gene94553:CDS
GTGCTGCTTTCAACTCTCCT+AGG	0.501482	4.1:+384299	MS.gene94553:CDS
AGATTTCTATGACGTCAGTC+TGG	0.501918	4.1:+384120	MS.gene94553:CDS
AGGGAATAAGCTTTGTTTGG+TGG	0.502787	4.1:-383926	None:intergenic
TCTCTCTTCTGCAACGGCAT+AGG	0.508531	4.1:+384049	MS.gene94553:CDS
TTCCTAATCGCATACCCTCC+AGG	0.509059	4.1:+383650	MS.gene94553:CDS
TCTCCTAGGTATTGCTGCAC+TGG	0.513261	4.1:+384313	MS.gene94553:CDS
GCCCAGTAGGGTTGCAGGTG+AGG	0.513868	4.1:+384236	MS.gene94553:CDS
CAAGTCCCACCACAATGGTC+AGG	0.514556	4.1:+383950	MS.gene94553:CDS
TCTGGAAAATGTAGCTATGC+TGG	0.515580	4.1:+384187	MS.gene94553:CDS
GTGAACTCAGCGAGTGTGGC+TGG	0.529115	4.1:-384082	None:intergenic
CTCTTCTGCAACGGCATAGG+CGG	0.529119	4.1:+384052	MS.gene94553:CDS
ACTCAGCGAGTGTGGCTGGT+GGG	0.531396	4.1:-384078	None:intergenic
AGTGATCATGTAACTAGCAT+TGG	0.535613	4.1:-384452	None:intergenic
CTCAGCGAGTGTGGCTGGTG+GGG	0.539267	4.1:-384077	None:intergenic
CTTAACTTGATGTGCCCAGT+AGG	0.539656	4.1:+384223	MS.gene94553:CDS
TGGGAGGTTGAAGCCGCCGC+GGG	0.541485	4.1:-383906	None:intergenic
GTGCAAGTAGCAATACTGGT+AGG	0.548800	4.1:-384430	None:intergenic
AAGATTCTTGAATAAGCAGT+GGG	0.550542	4.1:-384364	None:intergenic
CGTCGAAGGTGCAGCCGTGA+CGG	0.550989	4.1:-383985	None:intergenic
AACTCAGCGAGTGTGGCTGG+TGG	0.563622	4.1:-384079	None:intergenic
GATTGCTGGCCACACTGGGT+GGG	0.567882	4.1:-383855	None:intergenic
CAATGGTCAGGCCGTTTCTG+GGG	0.570580	4.1:+383962	MS.gene94553:CDS
CTTCAAAACTGCTTGCCCAA+AGG	0.572482	4.1:+384384	MS.gene94553:CDS
GGTGAAGATGGAAGCAGCCA+TGG	0.573489	4.1:-383609	None:intergenic
GAAGATTCTTGAATAAGCAG+TGG	0.575322	4.1:-384365	None:intergenic
CATTGCGCCACCGGAGACTG+CGG	0.577164	4.1:+384022	MS.gene94553:CDS
GTGTGGCCAGCAATCCAACC+CGG	0.580526	4.1:+383863	MS.gene94553:CDS
ATGATCAACGGCGGTGACGA+GGG	0.581144	4.1:-384480	None:intergenic
GCCGTGGCCTGATGCGTCGA+AGG	0.582247	4.1:-383999	None:intergenic
TTAACTTGATGTGCCCAGTA+GGG	0.582603	4.1:+384224	MS.gene94553:CDS
AATAAGCTTTGTTTGGTGGG+AGG	0.584207	4.1:-383922	None:intergenic
ACCTTCGACGCATCAGGCCA+CGG	0.592438	4.1:+383998	MS.gene94553:CDS
ATTTCCATTACACCTTTCAA+GGG	0.595357	4.1:+384163	MS.gene94553:CDS
GTGGGAGGTTGAAGCCGCCG+CGG	0.600609	4.1:-383907	None:intergenic
GATGATCAACGGCGGTGACG+AGG	0.606071	4.1:-384481	None:intergenic
TATGACGTCAGTCTGGTCGA+CGG	0.608080	4.1:+384127	MS.gene94553:CDS
GGCGGATCTCTCTTCTGCAA+CGG	0.609526	4.1:+384043	MS.gene94553:CDS
TGCAAGTAGCAATACTGGTA+GGG	0.611972	4.1:-384429	None:intergenic
AAACGGCCTGACCATTGTGG+TGG	0.613091	4.1:-383956	None:intergenic
TGCGCCACCGGAGACTGCGG+CGG	0.616529	4.1:+384025	MS.gene94553:CDS
TAATAAAATAAATACCTGGA+GGG	0.617393	4.1:-383664	None:intergenic
AGTGGGCTTGCAGGTCTGTG+GGG	0.617801	4.1:-384347	None:intergenic
TTACCAGTGCAGCAATACCT+AGG	0.621497	4.1:-384316	None:intergenic
AGATCCGCCGCAGTCTCCGG+TGG	0.621709	4.1:-384029	None:intergenic
CGTGACAAGAAGCACGTGGT+GGG	0.626207	4.1:+384262	MS.gene94553:CDS
CAGAAACGGCCTGACCATTG+TGG	0.626293	4.1:-383959	None:intergenic
AACGGCCTGACCATTGTGGT+GGG	0.629227	4.1:-383955	None:intergenic
GGAAAACCCATTCTGGCCCG+CGG	0.640848	4.1:+383890	MS.gene94553:CDS
AAATGTGAACTCAGCGAGTG+TGG	0.640994	4.1:-384086	None:intergenic
CCCTTCAAGTCCCACCACAA+TGG	0.642784	4.1:+383945	MS.gene94553:CDS
AAACCCATTCTGGCCCGCGG+CGG	0.643152	4.1:+383893	MS.gene94553:CDS
GAAAGCAGCGAAGAAGAGAG+CGG	0.656778	4.1:-383630	None:intergenic
ACGTGACAAGAAGCACGTGG+TGG	0.676547	4.1:+384261	MS.gene94553:CDS
ACAAGTGTTCCCACCCAGTG+TGG	0.681402	4.1:+383846	MS.gene94553:CDS
CCATTGTGGTGGGACTTGAA+GGG	0.705505	4.1:-383945	None:intergenic
TGAAGATAGATGATCAACGG+CGG	0.740375	4.1:-384489	None:intergenic
GTCACGTGACAAGAAGCACG+TGG	0.757070	4.1:+384258	MS.gene94553:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATAATAAAATAAATACC+TGG	-	chr4.1:383671-383690	None:intergenic	10.0%
!!	ATAATAAAATAAATACCTGG+AGG	-	chr4.1:383668-383687	None:intergenic	20.0%
!!	TAATAAAATAAATACCTGGA+GGG	-	chr4.1:383667-383686	None:intergenic	20.0%
!	AGAAACGACAAATTAAGTAA+CGG	-	chr4.1:383767-383786	None:intergenic	25.0%
!!!	ATTTTAACAAACAGTAGTGA+AGG	+	chr4.1:383793-383812	MS.gene94553:intron	25.0%
	AAGATTCTTGAATAAGCAGT+GGG	-	chr4.1:384367-384386	None:intergenic	30.0%
	ATTTCCATTACACCTTTCAA+GGG	+	chr4.1:384163-384182	MS.gene94553:CDS	30.0%
!	TTGAAAGGTGTAATGGAAAT+CGG	-	chr4.1:384163-384182	None:intergenic	30.0%
	AAGCATAAGAGTAAGCCTTT+GGG	-	chr4.1:384402-384421	None:intergenic	35.0%
	AGTGATCATGTAACTAGCAT+TGG	-	chr4.1:384455-384474	None:intergenic	35.0%
	GAAGATTCTTGAATAAGCAG+TGG	-	chr4.1:384368-384387	None:intergenic	35.0%
	GATTTCCATTACACCTTTCA+AGG	+	chr4.1:384162-384181	MS.gene94553:CDS	35.0%
	TAAGCATAAGAGTAAGCCTT+TGG	-	chr4.1:384403-384422	None:intergenic	35.0%
!!	TGAAGGGAATAAGCTTTGTT+TGG	-	chr4.1:383932-383951	None:intergenic	35.0%
	AGATTTCTATGACGTCAGTC+TGG	+	chr4.1:384120-384139	MS.gene94553:CDS	40.0%
	ATTACACCTTTCAAGGGATC+TGG	+	chr4.1:384169-384188	MS.gene94553:CDS	40.0%
	ATTGGTGCAAGTAGCAATAC+TGG	-	chr4.1:384437-384456	None:intergenic	40.0%
	TCTGGAAAATGTAGCTATGC+TGG	+	chr4.1:384187-384206	MS.gene94553:CDS	40.0%
	TGCAAGTAGCAATACTGGTA+GGG	-	chr4.1:384432-384451	None:intergenic	40.0%
!	AGATCCCTTGAAAGGTGTAA+TGG	-	chr4.1:384170-384189	None:intergenic	40.0%
!	CATTTTCCAGATCCCTTGAA+AGG	-	chr4.1:384178-384197	None:intergenic	40.0%
!	TATTGCTGCACTGGTAACTT+TGG	+	chr4.1:384322-384341	MS.gene94553:CDS	40.0%
!	TTAACTTGATGTGCCCAGTA+GGG	+	chr4.1:384224-384243	MS.gene94553:CDS	40.0%
!!	AATAAGCTTTGTTTGGTGGG+AGG	-	chr4.1:383925-383944	None:intergenic	40.0%
!!	AGGGAATAAGCTTTGTTTGG+TGG	-	chr4.1:383929-383948	None:intergenic	40.0%
!!	GGGAATAAGCTTTGTTTGGT+GGG	-	chr4.1:383928-383947	None:intergenic	40.0%
	AAATGTGAACTCAGCGAGTG+TGG	-	chr4.1:384089-384108	None:intergenic	45.0%
	ACACTCGCTGAGTTCACATT+TGG	+	chr4.1:384088-384107	MS.gene94553:CDS	45.0%
	CACTCGCTGAGTTCACATTT+GGG	+	chr4.1:384089-384108	MS.gene94553:CDS	45.0%
	CTTCAAAACTGCTTGCCCAA+AGG	+	chr4.1:384384-384403	MS.gene94553:CDS	45.0%
	GTGCAAGTAGCAATACTGGT+AGG	-	chr4.1:384433-384452	None:intergenic	45.0%
	TCACACACACACACAGAAAG+CGG	+	chr4.1:383710-383729	MS.gene94553:intron	45.0%
	TGTGTGTGAGAGAGAAGAGA+AGG	-	chr4.1:383699-383718	None:intergenic	45.0%
	TTACCAGTGCAGCAATACCT+AGG	-	chr4.1:384319-384338	None:intergenic	45.0%
!	CTTAACTTGATGTGCCCAGT+AGG	+	chr4.1:384223-384242	MS.gene94553:CDS	45.0%
	ACAATGGTCAGGCCGTTTCT+GGG	+	chr4.1:383961-383980	MS.gene94553:CDS	50.0%
	GAAAGCAGCGAAGAAGAGAG+CGG	-	chr4.1:383633-383652	None:intergenic	50.0%
	GAAGAAGAGAGCGGTGAAGA+TGG	-	chr4.1:383624-383643	None:intergenic	50.0%
	GTGTGAGAGAGAAGAGAAGG+AGG	-	chr4.1:383696-383715	None:intergenic	50.0%
	TATGACGTCAGTCTGGTCGA+CGG	+	chr4.1:384127-384146	MS.gene94553:CDS	50.0%
	TCTCTCTTCTGCAACGGCAT+AGG	+	chr4.1:384049-384068	MS.gene94553:CDS	50.0%
	TTCCTAATCGCATACCCTCC+AGG	+	chr4.1:383650-383669	MS.gene94553:CDS	50.0%
!	ACCATTGTGGTGGGACTTGA+AGG	-	chr4.1:383949-383968	None:intergenic	50.0%
!	CCATTGTGGTGGGACTTGAA+GGG	-	chr4.1:383948-383967	None:intergenic	50.0%
!	TACCTGGAGGGTATGCGATT+AGG	-	chr4.1:383655-383674	None:intergenic	50.0%
!	TCTCCTAGGTATTGCTGCAC+TGG	+	chr4.1:384313-384332	MS.gene94553:CDS	50.0%
!	TGAATAAGCAGTGGGCTTGC+AGG	-	chr4.1:384359-384378	None:intergenic	50.0%
!!	GTGCTGCTTTCAACTCTCCT+AGG	+	chr4.1:384299-384318	MS.gene94553:CDS	50.0%
	AAACGGCCTGACCATTGTGG+TGG	-	chr4.1:383959-383978	None:intergenic	55.0%
	AACGGCCTGACCATTGTGGT+GGG	-	chr4.1:383958-383977	None:intergenic	55.0%
	ACAAGTGTTCCCACCCAGTG+TGG	+	chr4.1:383846-383865	MS.gene94553:CDS	55.0%
	ACCTCACCTGCAACCCTACT+GGG	-	chr4.1:384240-384259	None:intergenic	55.0%
	CAAGTCCCACCACAATGGTC+AGG	+	chr4.1:383950-383969	MS.gene94553:CDS	55.0%
	CAATGGTCAGGCCGTTTCTG+GGG	+	chr4.1:383962-383981	MS.gene94553:CDS	55.0%
	CACAATGGTCAGGCCGTTTC+TGG	+	chr4.1:383960-383979	MS.gene94553:CDS	55.0%
	CAGAAACGGCCTGACCATTG+TGG	-	chr4.1:383962-383981	None:intergenic	55.0%
	CCCTTCAAGTCCCACCACAA+TGG	+	chr4.1:383945-383964	MS.gene94553:CDS	55.0%
	CTCTTCTGCAACGGCATAGG+CGG	+	chr4.1:384052-384071	MS.gene94553:CDS	55.0%
	GGCGGATCTCTCTTCTGCAA+CGG	+	chr4.1:384043-384062	MS.gene94553:CDS	55.0%
	GTTGGATTGCTGGCCACACT+GGG	-	chr4.1:383862-383881	None:intergenic	55.0%
!	ACGTGACAAGAAGCACGTGG+TGG	+	chr4.1:384261-384280	MS.gene94553:CDS	55.0%
!	CGTGACAAGAAGCACGTGGT+GGG	+	chr4.1:384262-384281	MS.gene94553:CDS	55.0%
!	GTCACGTGACAAGAAGCACG+TGG	+	chr4.1:384258-384277	MS.gene94553:CDS	55.0%
!!	AGAATGGGTTTTCCGGCACC+GGG	-	chr4.1:383884-383903	None:intergenic	55.0%
!!	CAGAATGGGTTTTCCGGCAC+CGG	-	chr4.1:383885-383904	None:intergenic	55.0%
	AACTCAGCGAGTGTGGCTGG+TGG	-	chr4.1:384082-384101	None:intergenic	60.0%
	ACCTTCGACGCATCAGGCCA+CGG	+	chr4.1:383998-384017	MS.gene94553:CDS	60.0%
	ACTCAGCGAGTGTGGCTGGT+GGG	-	chr4.1:384081-384100	None:intergenic	60.0%
	GACCTCACCTGCAACCCTAC+TGG	-	chr4.1:384241-384260	None:intergenic	60.0%
	GATGTGCCCAGTAGGGTTGC+AGG	+	chr4.1:384231-384250	MS.gene94553:CDS	60.0%
	GATTGCTGGCCACACTGGGT+GGG	-	chr4.1:383858-383877	None:intergenic	60.0%
	GGAAAACCCATTCTGGCCCG+CGG	+	chr4.1:383890-383909	MS.gene94553:CDS	60.0%
	GGTTGGATTGCTGGCCACAC+TGG	-	chr4.1:383863-383882	None:intergenic	60.0%
	GTGAACTCAGCGAGTGTGGC+TGG	-	chr4.1:384085-384104	None:intergenic	60.0%
	GTGTGGCCAGCAATCCAACC+CGG	+	chr4.1:383863-383882	MS.gene94553:CDS	60.0%
!	AGTGGGCTTGCAGGTCTGTG+GGG	-	chr4.1:384350-384369	None:intergenic	60.0%
!	CAGTGGGCTTGCAGGTCTGT+GGG	-	chr4.1:384351-384370	None:intergenic	60.0%
!	CGGTGCCGGAAAACCCATTC+TGG	+	chr4.1:383883-383902	MS.gene94553:CDS	60.0%
!!	GCGGGCCAGAATGGGTTTTC+CGG	-	chr4.1:383891-383910	None:intergenic	60.0%
	AAACCCATTCTGGCCCGCGG+CGG	+	chr4.1:383893-383912	MS.gene94553:CDS	65.0%
	AGCCGTGACGGCCCCAGAAA+CGG	-	chr4.1:383976-383995	None:intergenic	65.0%
	CATTGCGCCACCGGAGACTG+CGG	+	chr4.1:384022-384041	MS.gene94553:CDS	65.0%
	CCAGCAATCCAACCCGGTGC+CGG	+	chr4.1:383869-383888	MS.gene94553:CDS	65.0%
	CTCAGCGAGTGTGGCTGGTG+GGG	-	chr4.1:384080-384099	None:intergenic	65.0%
	GAGAGATCCGCCGCAGTCTC+CGG	-	chr4.1:384035-384054	None:intergenic	65.0%
	GCCCAGTAGGGTTGCAGGTG+AGG	+	chr4.1:384236-384255	MS.gene94553:CDS	65.0%
	GGATTGCTGGCCACACTGGG+TGG	-	chr4.1:383859-383878	None:intergenic	65.0%
	GGCTGCACCTTCGACGCATC+AGG	+	chr4.1:383992-384011	MS.gene94553:CDS	65.0%
!	CGTCGAAGGTGCAGCCGTGA+CGG	-	chr4.1:383988-384007	None:intergenic	65.0%
!	GCAGTGGGCTTGCAGGTCTG+TGG	-	chr4.1:384352-384371	None:intergenic	65.0%
!!	TGGGTTTTCCGGCACCGGGT+TGG	-	chr4.1:383880-383899	None:intergenic	65.0%
	AAGCCGCCGCGGGCCAGAAT+GGG	-	chr4.1:383899-383918	None:intergenic	70.0%
	AGATCCGCCGCAGTCTCCGG+TGG	-	chr4.1:384032-384051	None:intergenic	70.0%
	CGCAGTCTCCGGTGGCGCAA+TGG	-	chr4.1:384024-384043	None:intergenic	70.0%
	GCCGTGGCCTGATGCGTCGA+AGG	-	chr4.1:384002-384021	None:intergenic	70.0%
	GTGGGAGGTTGAAGCCGCCG+CGG	-	chr4.1:383910-383929	None:intergenic	70.0%
	TGGGAGGTTGAAGCCGCCGC+GGG	-	chr4.1:383909-383928	None:intergenic	70.0%
!	CCGGCACCGGGTTGGATTGC+TGG	-	chr4.1:383872-383891	None:intergenic	70.0%
!!	GGCCGTTTCTGGGGCCGTCA+CGG	+	chr4.1:383971-383990	MS.gene94553:CDS	70.0%
	CTCCGGTGGCGCAATGGCCG+TGG	-	chr4.1:384018-384037	None:intergenic	75.0%
	GAAGCCGCCGCGGGCCAGAA+TGG	-	chr4.1:383900-383919	None:intergenic	75.0%
	GGCCACGGCCATTGCGCCAC+CGG	+	chr4.1:384013-384032	MS.gene94553:CDS	75.0%
	TGCGCCACCGGAGACTGCGG+CGG	+	chr4.1:384025-384044	MS.gene94553:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	383611	384498	383611	ID=MS.gene94553
chr4.1	mRNA	383611	384498	383611	ID=MS.gene94553.t1;Parent=MS.gene94553
chr4.1	exon	383611	383671	383611	ID=MS.gene94553.t1.exon1;Parent=MS.gene94553.t1
chr4.1	CDS	383611	383671	383611	ID=cds.MS.gene94553.t1;Parent=MS.gene94553.t1
chr4.1	exon	383807	384498	383807	ID=MS.gene94553.t1.exon2;Parent=MS.gene94553.t1
chr4.1	CDS	383807	384498	383807	ID=cds.MS.gene94553.t1;Parent=MS.gene94553.t1

Gene Sequence

>MS.gene94553

ATGGCTGCTTCCATCTTCACCGCTCTCTTCTTCGCTGCTTTCCTAATCGCATACCCTCCAGGTATTTATTTTATTATTATTTCCTCCTTCTCTTCTCTCTCACACACACACACAGAAAGCGGTTTGTGAGAGATATATAGTACATTTATTACACCGTTACTTAATTTGTCGTTTCTTGTTTGATTTTAACAAACAGTAGTGAAGGTATCAGCAGCAGCATCAGTAGTATTTTACAACAAGTGTTCCCACCCAGTGTGGCCAGCAATCCAACCCGGTGCCGGAAAACCCATTCTGGCCCGCGGCGGCTTCAACCTCCCACCAAACAAAGCTTATTCCCTTCAAGTCCCACCACAATGGTCAGGCCGTTTCTGGGGCCGTCACGGCTGCACCTTCGACGCATCAGGCCACGGCCATTGCGCCACCGGAGACTGCGGCGGATCTCTCTTCTGCAACGGCATAGGCGGAACCCCACCAGCCACACTCGCTGAGTTCACATTTGGGAAAGATCTAGATTTCTATGACGTCAGTCTGGTCGACGGTTACAACTTACCGATTTCCATTACACCTTTCAAGGGATCTGGAAAATGTAGCTATGCTGGCTGTGTGAGTGATCTTAACTTGATGTGCCCAGTAGGGTTGCAGGTGAGGTCACGTGACAAGAAGCACGTGGTGGGTTGTAGAAGTGCTTGTGCTGCTTTCAACTCTCCTAGGTATTGCTGCACTGGTAACTTTGGTACCCCACAGACCTGCAAGCCCACTGCTTATTCAAGAATCTTCAAAACTGCTTGCCCAAAGGCTTACTCTTATGCTTATGATGACCCTACCAGTATTGCTACTTGCACCAATGCTAGTTACATGATCACTTTCTGCCCTCGTCACCGCCGTTGA

Protein sequence

>MS.gene94553.t1

MAASIFTALFFAAFLIAYPPVVKVSAAASVVFYNKCSHPVWPAIQPGAGKPILARGGFNLPPNKAYSLQVPPQWSGRFWGRHGCTFDASGHGHCATGDCGGSLFCNGIGGTPPATLAEFTFGKDLDFYDVSLVDGYNLPISITPFKGSGKCSYAGCVSDLNLMCPVGLQVRSRDKKHVVGCRSACAAFNSPRYCCTGNFGTPQTCKPTAYSRIFKTACPKAYSYAYDDPTSIATCTNASYMITFCPRHRR