AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040126

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040126.t1 MTR_8g107140 98.000 250 4 1 1 250 1 249 0.0 500
MS.gene040126.t1 MTR_6g079580 69.748 238 71 1 9 246 4 240 3.39e-130 368
MS.gene040126.t1 MTR_8g036215 59.091 220 88 2 27 245 19 237 1.64e-91 270
MS.gene040126.t1 MTR_3g111620 54.626 227 98 3 23 246 22 246 1.33e-87 260
MS.gene040126.t1 MTR_8g075550 54.506 233 96 4 27 250 27 258 7.69e-84 253
MS.gene040126.t1 MTR_4g073720 51.639 244 102 5 4 245 10 239 4.88e-82 249
MS.gene040126.t1 MTR_1g025420 52.893 242 109 4 9 245 4 245 7.97e-82 248
MS.gene040126.t1 MTR_1g025420 52.893 242 109 4 9 245 4 245 1.16e-81 246
MS.gene040126.t1 MTR_7g062610 50.000 236 112 3 14 246 10 242 3.01e-81 244
MS.gene040126.t1 MTR_3g068015 49.597 248 115 4 7 246 3 248 4.91e-81 243
MS.gene040126.t1 MTR_1g021945 54.148 229 98 3 23 247 21 246 1.21e-80 243
MS.gene040126.t1 MTR_8g075510 53.750 240 99 6 10 246 14 244 2.00e-80 242
MS.gene040126.t1 MTR_5g022350 54.709 223 91 4 26 246 29 243 8.56e-80 243
MS.gene040126.t1 MTR_8g075510 53.556 239 99 6 10 245 14 243 1.34e-79 242
MS.gene040126.t1 MTR_8g037890 49.801 251 117 5 1 246 1 247 3.75e-78 238
MS.gene040126.t1 MTR_5g059200 53.097 226 99 4 25 250 26 244 2.70e-77 235
MS.gene040126.t1 MTR_5g022310 50.198 253 117 6 1 246 1 251 2.08e-76 232
MS.gene040126.t1 MTR_5g022310 50.198 253 117 6 1 246 1 251 4.24e-76 233
MS.gene040126.t1 MTR_7g102380 52.679 224 100 5 28 247 21 242 5.07e-76 231
MS.gene040126.t1 MTR_3g114030 50.218 229 109 4 27 250 6 234 4.71e-73 223
MS.gene040126.t1 MTR_1g062590 47.325 243 122 5 8 246 2 242 9.11e-73 222
MS.gene040126.t1 MTR_8g056820 48.413 252 119 6 1 246 1 247 9.37e-73 223
MS.gene040126.t1 MTR_6g009480 44.664 253 132 6 1 247 3 253 2.81e-72 222
MS.gene040126.t1 MTR_2g069660 50.885 226 106 4 25 246 19 243 6.56e-71 218
MS.gene040126.t1 MTR_1g062370 48.117 239 118 5 12 246 6 242 2.01e-70 216
MS.gene040126.t1 MTR_1g062350 47.699 239 119 5 12 246 6 242 9.96e-70 214
MS.gene040126.t1 MTR_4g073730 49.794 243 115 6 9 246 8 248 1.24e-69 216
MS.gene040126.t1 MTR_1g062340 47.899 238 118 5 13 246 7 242 1.56e-69 214
MS.gene040126.t1 MTR_4g073730 49.794 243 115 6 9 246 8 248 3.21e-69 216
MS.gene040126.t1 MTR_1g062660 48.458 227 111 5 26 248 20 244 1.82e-68 211
MS.gene040126.t1 MTR_1g062630 45.528 246 128 5 8 249 2 245 7.18e-68 210
MS.gene040126.t1 MTR_2g068030 46.725 229 115 5 22 245 17 243 5.04e-67 208
MS.gene040126.t1 MTR_2g067980 46.939 245 118 6 9 246 8 247 1.39e-64 217
MS.gene040126.t1 MTR_4g063630 44.958 238 120 6 14 245 19 251 2.26e-62 196
MS.gene040126.t1 MTR_2g068655 44.541 229 120 5 27 250 30 256 2.07e-60 193
MS.gene040126.t1 MTR_1g062690 43.038 237 109 5 12 246 6 218 1.75e-57 182
MS.gene040126.t1 MTR_5g010635 41.333 225 109 7 25 246 21 225 1.78e-49 162
MS.gene040126.t1 MTR_5g010640 38.340 253 128 8 4 246 2 236 3.43e-48 159
MS.gene040126.t1 MTR_2g067970 45.902 183 89 7 71 246 3 182 4.51e-47 167
MS.gene040126.t1 MTR_8g096900 36.948 249 125 11 3 246 27 248 5.22e-45 151
MS.gene040126.t1 MTR_8g089020 48.936 141 69 2 101 241 22 159 5.56e-43 143
MS.gene040126.t1 MTR_5g022350 53.030 132 57 3 26 155 94 222 1.34e-40 139
MS.gene040126.t1 MTR_2g067990 37.500 224 109 9 27 246 26 222 1.20e-39 146
MS.gene040126.t1 MTR_2g063150 33.659 205 128 5 28 228 34 234 1.28e-35 127
MS.gene040126.t1 MTR_5g023850 34.400 250 119 10 7 247 4 217 5.94e-35 125
MS.gene040126.t1 MTR_1g062390 30.830 253 153 7 1 246 1 238 5.93e-30 112
MS.gene040126.t1 MTR_7g076360 34.123 211 76 4 27 236 7 155 1.01e-29 110
MS.gene040126.t1 MTR_8g096910 39.860 143 71 5 4 143 2 132 1.50e-27 104
MS.gene040126.t1 MTR_8g096920 40.146 137 70 5 11 143 1 129 1.56e-24 96.3
MS.gene040126.t1 MTR_3g081550 42.553 94 51 2 112 202 16 109 1.71e-15 70.9
MS.gene040126.t1 MTR_1g062640 31.507 146 90 5 25 167 10 148 3.69e-14 68.6
MS.gene040126.t1 MTR_5g068670 48.750 80 37 2 65 142 12 89 4.14e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040126.t1 AT1G18250 77.974 227 50 0 24 250 18 244 9.29e-135 379
MS.gene040126.t1 AT1G18250 77.434 226 51 0 25 250 18 243 6.37e-134 377
MS.gene040126.t1 AT1G73620 73.894 226 59 0 25 250 39 264 4.37e-129 366
MS.gene040126.t1 AT1G75050 55.882 238 101 3 14 249 11 246 3.60e-88 261
MS.gene040126.t1 AT1G75030 55.947 227 95 3 26 249 22 246 4.84e-85 254
MS.gene040126.t1 AT1G75040 56.250 240 94 6 9 246 9 239 7.73e-84 250
MS.gene040126.t1 AT1G19320 54.321 243 100 5 9 245 9 246 3.02e-82 246
MS.gene040126.t1 AT4G38660 51.304 230 105 3 21 248 1 225 1.05e-80 245
MS.gene040126.t1 AT4G38660 49.597 248 114 4 3 248 9 247 2.49e-80 245
MS.gene040126.t1 AT1G77700 53.712 229 98 5 23 250 55 276 1.17e-79 242
MS.gene040126.t1 AT1G77700 54.911 224 93 5 23 245 55 271 2.62e-79 242
MS.gene040126.t1 AT1G77700 53.680 231 99 5 16 245 76 299 2.81e-79 243
MS.gene040126.t1 AT1G75800 52.917 240 106 5 14 246 9 248 1.99e-77 237
MS.gene040126.t1 AT1G20030 54.667 225 95 5 29 246 4 228 2.25e-76 233
MS.gene040126.t1 AT1G20030 54.667 225 95 5 29 246 21 245 3.10e-76 234
MS.gene040126.t1 AT4G24180 48.047 256 112 6 5 250 16 260 1.23e-74 228
MS.gene040126.t1 AT5G02140 47.280 239 120 4 12 246 5 241 3.87e-74 228
MS.gene040126.t1 AT4G24180 48.413 252 109 6 5 246 16 256 4.41e-74 228
MS.gene040126.t1 AT4G24180 50.000 236 103 5 25 250 25 255 4.67e-74 226
MS.gene040126.t1 AT5G02140 47.280 239 120 4 12 246 5 241 6.06e-74 225
MS.gene040126.t1 AT2G17860 47.755 245 120 5 14 250 9 253 1.64e-73 224
MS.gene040126.t1 AT4G24180 48.971 243 109 5 14 246 14 251 2.20e-73 226
MS.gene040126.t1 AT5G40020 45.902 244 126 3 10 248 8 250 4.59e-73 223
MS.gene040126.t1 AT4G36010 51.082 231 102 5 26 246 21 250 4.16e-72 223
MS.gene040126.t1 AT4G36010 51.082 231 102 5 26 246 21 250 4.16e-72 223
MS.gene040126.t1 AT5G24620 46.718 259 118 5 1 250 9 256 4.35e-72 226
MS.gene040126.t1 AT5G24620 46.718 259 118 5 1 250 64 311 1.11e-71 227
MS.gene040126.t1 AT5G24620 46.718 259 118 5 1 250 85 332 1.35e-71 227
MS.gene040126.t1 AT4G38670 48.387 248 115 6 5 246 7 247 5.06e-69 214
MS.gene040126.t1 AT4G38670 48.387 248 115 6 5 246 7 247 5.06e-69 214
MS.gene040126.t1 AT4G38670 51.339 224 103 5 29 246 8 231 8.52e-68 210
MS.gene040126.t1 AT4G24180 46.835 237 105 6 5 231 16 241 2.81e-65 206
MS.gene040126.t1 AT2G28790 44.958 238 122 6 14 245 39 273 3.00e-65 204
MS.gene040126.t1 AT2G28790 44.958 238 122 6 14 245 14 248 3.98e-65 203
MS.gene040126.t1 AT4G24180 48.848 217 96 5 25 231 25 236 1.06e-64 204
MS.gene040126.t1 AT5G38280 44.167 240 127 6 14 246 11 250 4.67e-60 201
MS.gene040126.t1 AT5G38280 44.167 240 127 6 14 246 11 250 6.03e-60 201
MS.gene040126.t1 AT4G11650 40.239 251 123 7 1 249 1 226 1.22e-56 181
MS.gene040126.t1 AT4G36000 58.503 147 51 3 103 247 1 139 2.03e-54 174
MS.gene040126.t1 AT4G36000 58.503 147 51 3 103 247 1 139 2.03e-54 174
MS.gene040126.t1 AT4G18253 39.076 238 137 4 14 249 10 241 3.41e-51 167
MS.gene040126.t1 AT1G70250 38.767 227 125 8 29 246 146 367 1.10e-42 154
MS.gene040126.t1 AT4G18250 36.864 236 140 6 14 246 43 272 2.15e-38 142
MS.gene040126.t1 AT5G24620 52.800 125 53 3 132 250 15 139 2.74e-35 128
MS.gene040126.t1 AT2G24810 58.442 77 32 0 169 245 116 192 1.75e-24 97.1

Find 98 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATGGTCAGGCCGTTTC+TGG 0.068116 4.4:+396533 MS.gene040126:CDS
CCGGCACCGGGTTGGATTGC+TGG 0.183097 4.4:-396442 None:intergenic
TGAATAAGCAGTGGGCTTGC+AGG 0.307584 4.4:-396929 None:intergenic
GACCTCACCTGCAACCCTAC+TGG 0.350758 4.4:-396811 None:intergenic
ACACTCGCTGAGTTCACATT+TGG 0.368237 4.4:+396661 MS.gene040126:CDS
CGGTGCCGGAAAACCCATTC+TGG 0.376903 4.4:+396456 MS.gene040126:CDS
CTGACCATTGTGGTGGGACT+TGG 0.379600 4.4:-396522 None:intergenic
GAAGCCGCCGCGGGCCAGAA+TGG 0.391505 4.4:-396470 None:intergenic
ACAATGGTCAGGCCGTTTCT+GGG 0.391880 4.4:+396534 MS.gene040126:CDS
ATTGCTGGCCACACTGGGTG+GGG 0.395326 4.4:-396427 None:intergenic
TGGAGGGAATAAGCTTTGTT+TGG 0.398076 4.4:-396502 None:intergenic
ATTACACCATTCAAGGGATC+TGG 0.398119 4.4:+396742 MS.gene040126:CDS
TAAGCATAAGAGTAAGCCTT+TGG 0.402209 4.4:-396973 None:intergenic
GGTTGGATTGCTGGCCACAC+TGG 0.402359 4.4:-396433 None:intergenic
GGGAATAAGCTTTGTTTGGT+GGG 0.404140 4.4:-396498 None:intergenic
CACTCGCTGAGTTCACATTT+GGG 0.419380 4.4:+396662 MS.gene040126:CDS
AAGCCGCCGCGGGCCAGAAT+GGG 0.420312 4.4:-396469 None:intergenic
GATGTGCCCAGTAGGGTTGC+AGG 0.421685 4.4:+396804 MS.gene040126:CDS
CGCAGTCTCCGGTGGCGCAA+TGG 0.424632 4.4:-396594 None:intergenic
TTGAATGGTGTAATGGAAAT+CGG 0.432583 4.4:-396733 None:intergenic
CAGTGGGCTTGCAGGTCTGT+GGG 0.433412 4.4:-396921 None:intergenic
ATTGGTGCAAGTAGCAATAC+TGG 0.439699 4.4:-397007 None:intergenic
ACCTCACCTGCAACCCTACT+GGG 0.443228 4.4:-396810 None:intergenic
GGCCACGGCCATTGCGCCAC+CGG 0.444546 4.4:+396586 MS.gene040126:CDS
GGATTGCTGGCCACACTGGG+TGG 0.446029 4.4:-396429 None:intergenic
CTCCGGTGGCGCAATGGCCG+TGG 0.450871 4.4:-396588 None:intergenic
GTTGGATTGCTGGCCACACT+GGG 0.457650 4.4:-396432 None:intergenic
ACCATTGTGGTGGGACTTGG+AGG 0.458699 4.4:-396519 None:intergenic
GATTGCTGGCCACACTGGGT+GGG 0.464130 4.4:-396428 None:intergenic
TACCTGGAGGGTATGCGATT+AGG 0.464838 4.4:-396222 None:intergenic
GCAGTGGGCTTGCAGGTCTG+TGG 0.469723 4.4:-396922 None:intergenic
GATTTCCATTACACCATTCA+AGG 0.471423 4.4:+396735 MS.gene040126:CDS
GGCTGCACCTTCGACGCATC+AGG 0.474004 4.4:+396565 MS.gene040126:CDS
GAAGAAGAGAGCGGTGAAGA+TGG 0.478141 4.4:-396191 None:intergenic
GAGAGATCCGCCGCAGTCTC+CGG 0.481610 4.4:-396605 None:intergenic
AAGCATAAGAGTAAGCCTTT+GGG 0.484229 4.4:-396972 None:intergenic
CAAGTCCCACCACAATGGTC+AGG 0.493666 4.4:+396523 MS.gene040126:CDS
AGGGAATAAGCTTTGTTTGG+TGG 0.495351 4.4:-396499 None:intergenic
GGCCGTTTCTGGGGCCGTCA+CGG 0.495582 4.4:+396544 MS.gene040126:CDS
AGCCGTGACGGCCCCAGAAA+CGG 0.498382 4.4:-396546 None:intergenic
TATTGCTGCACTGGTAACTT+TGG 0.498988 4.4:+396895 MS.gene040126:CDS
CCAGCAATCCAACCCGGTGC+CGG 0.501469 4.4:+396442 MS.gene040126:CDS
GTGCTGCTTTCAACTCTCCT+AGG 0.501482 4.4:+396872 MS.gene040126:CDS
AGATCCCTTGAATGGTGTAA+TGG 0.501950 4.4:-396740 None:intergenic
TCTCTCTTCTGCAACGGCAT+AGG 0.508531 4.4:+396622 MS.gene040126:CDS
TTCCTAATCGCATACCCTCC+AGG 0.509059 4.4:+396220 MS.gene040126:CDS
TCTCCTAGGTATTGCTGCAC+TGG 0.513261 4.4:+396886 MS.gene040126:CDS
GCCCAGTAGGGTTGCAGGTG+AGG 0.513868 4.4:+396809 MS.gene040126:CDS
TCTGGAAAATGTAGCTATGC+TGG 0.515580 4.4:+396760 MS.gene040126:CDS
GTGAACTCAGCGAGTGTGGC+TGG 0.529115 4.4:-396655 None:intergenic
CTCTTCTGCAACGGCATAGG+CGG 0.529119 4.4:+396625 MS.gene040126:CDS
ACTCAGCGAGTGTGGCTGGT+GGG 0.531396 4.4:-396651 None:intergenic
AGTGATCATGTAACTAGCAT+TGG 0.535613 4.4:-397025 None:intergenic
CTCAGCGAGTGTGGCTGGTG+GGG 0.539267 4.4:-396650 None:intergenic
CTTAACTTGATGTGCCCAGT+AGG 0.539656 4.4:+396796 MS.gene040126:CDS
TGGGAGGTTGAAGCCGCCGC+GGG 0.541485 4.4:-396479 None:intergenic
GTGCAAGTAGCAATACTGGT+AGG 0.548800 4.4:-397003 None:intergenic
AAGATTCTTGAATAAGCAGT+GGG 0.550542 4.4:-396937 None:intergenic
CGTCGAAGGTGCAGCCGTGA+CGG 0.550989 4.4:-396558 None:intergenic
GTAAGTTGTAACCGTCGACC+AGG 0.554402 4.4:-396711 None:intergenic
AACTCAGCGAGTGTGGCTGG+TGG 0.563622 4.4:-396652 None:intergenic
AGATTTCTATGACGTCAGCC+TGG 0.567905 4.4:+396693 MS.gene040126:CDS
CAATGGTCAGGCCGTTTCTG+GGG 0.570580 4.4:+396535 MS.gene040126:CDS
CTTCAAAACTGCTTGCCCAA+AGG 0.572482 4.4:+396957 MS.gene040126:CDS
GGTGAAGATGGAAGCAGCCA+TGG 0.573489 4.4:-396179 None:intergenic
GAAGATTCTTGAATAAGCAG+TGG 0.575322 4.4:-396938 None:intergenic
CATTGCGCCACCGGAGACTG+CGG 0.577164 4.4:+396595 MS.gene040126:CDS
GTGTGGCCAGCAATCCAACC+CGG 0.580526 4.4:+396436 MS.gene040126:CDS
ATGATCAACGGCGGTGACGA+GGG 0.581144 4.4:-397053 None:intergenic
GCCGTGGCCTGATGCGTCGA+AGG 0.582247 4.4:-396572 None:intergenic
TTAACTTGATGTGCCCAGTA+GGG 0.582603 4.4:+396797 MS.gene040126:CDS
AATAAGCTTTGTTTGGTGGG+AGG 0.584207 4.4:-396495 None:intergenic
ACCTTCGACGCATCAGGCCA+CGG 0.592438 4.4:+396571 MS.gene040126:CDS
ATTTCCATTACACCATTCAA+GGG 0.595357 4.4:+396736 MS.gene040126:CDS
GTGGGAGGTTGAAGCCGCCG+CGG 0.600609 4.4:-396480 None:intergenic
TATGACGTCAGCCTGGTCGA+CGG 0.603237 4.4:+396700 MS.gene040126:CDS
GATGATCAACGGCGGTGACG+AGG 0.606071 4.4:-397054 None:intergenic
GGCGGATCTCTCTTCTGCAA+CGG 0.609526 4.4:+396616 MS.gene040126:CDS
TGCAAGTAGCAATACTGGTA+GGG 0.611972 4.4:-397002 None:intergenic
AAACGGCCTGACCATTGTGG+TGG 0.613091 4.4:-396529 None:intergenic
TGCGCCACCGGAGACTGCGG+CGG 0.616529 4.4:+396598 MS.gene040126:CDS
AGTGGGCTTGCAGGTCTGTG+GGG 0.617801 4.4:-396920 None:intergenic
TTACCAGTGCAGCAATACCT+AGG 0.621497 4.4:-396889 None:intergenic
AGATCCGCCGCAGTCTCCGG+TGG 0.621709 4.4:-396602 None:intergenic
CGTGACAAGAAGCACGTGGT+GGG 0.626207 4.4:+396835 MS.gene040126:CDS
CAGAAACGGCCTGACCATTG+TGG 0.626293 4.4:-396532 None:intergenic
AACGGCCTGACCATTGTGGT+GGG 0.629227 4.4:-396528 None:intergenic
CCATTGTGGTGGGACTTGGA+GGG 0.634133 4.4:-396518 None:intergenic
GGAAAACCCATTCTGGCCCG+CGG 0.640848 4.4:+396463 MS.gene040126:CDS
AAATGTGAACTCAGCGAGTG+TGG 0.640994 4.4:-396659 None:intergenic
CCCTCCAAGTCCCACCACAA+TGG 0.642784 4.4:+396518 MS.gene040126:CDS
AAACCCATTCTGGCCCGCGG+CGG 0.643152 4.4:+396466 MS.gene040126:CDS
GAAAGCAGCGAAGAAGAGAG+CGG 0.656778 4.4:-396200 None:intergenic
ACAAGTGTCCCCACCCAGTG+TGG 0.666782 4.4:+396419 MS.gene040126:CDS
ATAATAATAAAATACCTGGA+GGG 0.667763 4.4:-396234 None:intergenic
ACGTGACAAGAAGCACGTGG+TGG 0.676547 4.4:+396834 MS.gene040126:CDS
GTCACGTGACAAGAAGCACG+TGG 0.757070 4.4:+396831 MS.gene040126:CDS
ATACGATAGATGATCAACGG+CGG 0.782774 4.4:-397062 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAAAATACCTGG+AGG - chr4.4:396238-396257 None:intergenic 20.0%
!! AGAAACAACAAATTAAGTAA+CGG - chr4.4:396340-396359 None:intergenic 20.0%
!! ATAATAATAAAATACCTGGA+GGG - chr4.4:396237-396256 None:intergenic 20.0%
!! GGAAATAATAATAAAATACC+TGG - chr4.4:396241-396260 None:intergenic 20.0%
!!! ATTTTAACAAACAGTAGTGA+AGG + chr4.4:396366-396385 MS.gene040126:intron 25.0%
AAGATTCTTGAATAAGCAGT+GGG - chr4.4:396940-396959 None:intergenic 30.0%
ATTTCCATTACACCATTCAA+GGG + chr4.4:396736-396755 MS.gene040126:CDS 30.0%
! TTGAATGGTGTAATGGAAAT+CGG - chr4.4:396736-396755 None:intergenic 30.0%
AAGCATAAGAGTAAGCCTTT+GGG - chr4.4:396975-396994 None:intergenic 35.0%
AGTGATCATGTAACTAGCAT+TGG - chr4.4:397028-397047 None:intergenic 35.0%
GAAGATTCTTGAATAAGCAG+TGG - chr4.4:396941-396960 None:intergenic 35.0%
GATTTCCATTACACCATTCA+AGG + chr4.4:396735-396754 MS.gene040126:CDS 35.0%
TAAGCATAAGAGTAAGCCTT+TGG - chr4.4:396976-396995 None:intergenic 35.0%
AGATCCCTTGAATGGTGTAA+TGG - chr4.4:396743-396762 None:intergenic 40.0%
ATTACACCATTCAAGGGATC+TGG + chr4.4:396742-396761 MS.gene040126:CDS 40.0%
ATTGGTGCAAGTAGCAATAC+TGG - chr4.4:397010-397029 None:intergenic 40.0%
TCTGGAAAATGTAGCTATGC+TGG + chr4.4:396760-396779 MS.gene040126:CDS 40.0%
TGCAAGTAGCAATACTGGTA+GGG - chr4.4:397005-397024 None:intergenic 40.0%
! CATTTTCCAGATCCCTTGAA+TGG - chr4.4:396751-396770 None:intergenic 40.0%
! TATTGCTGCACTGGTAACTT+TGG + chr4.4:396895-396914 MS.gene040126:CDS 40.0%
! TTAACTTGATGTGCCCAGTA+GGG + chr4.4:396797-396816 MS.gene040126:CDS 40.0%
!! AATAAGCTTTGTTTGGTGGG+AGG - chr4.4:396498-396517 None:intergenic 40.0%
!! AGGGAATAAGCTTTGTTTGG+TGG - chr4.4:396502-396521 None:intergenic 40.0%
!! GGGAATAAGCTTTGTTTGGT+GGG - chr4.4:396501-396520 None:intergenic 40.0%
!! TGGAGGGAATAAGCTTTGTT+TGG - chr4.4:396505-396524 None:intergenic 40.0%
AAATGTGAACTCAGCGAGTG+TGG - chr4.4:396662-396681 None:intergenic 45.0%
ACACTCGCTGAGTTCACATT+TGG + chr4.4:396661-396680 MS.gene040126:CDS 45.0%
AGAGAGAAGAGAAGAGAAGG+AGG - chr4.4:396262-396281 None:intergenic 45.0%
AGATTTCTATGACGTCAGCC+TGG + chr4.4:396693-396712 MS.gene040126:CDS 45.0%
CACTCGCTGAGTTCACATTT+GGG + chr4.4:396662-396681 MS.gene040126:CDS 45.0%
CTTCAAAACTGCTTGCCCAA+AGG + chr4.4:396957-396976 MS.gene040126:CDS 45.0%
GAGAGAGAGAAGAGAAGAGA+AGG - chr4.4:396265-396284 None:intergenic 45.0%
GTGCAAGTAGCAATACTGGT+AGG - chr4.4:397006-397025 None:intergenic 45.0%
TCTCACACACACAGAGAAAG+CGG + chr4.4:396283-396302 MS.gene040126:intron 45.0%
TTACCAGTGCAGCAATACCT+AGG - chr4.4:396892-396911 None:intergenic 45.0%
! CTTAACTTGATGTGCCCAGT+AGG + chr4.4:396796-396815 MS.gene040126:CDS 45.0%
ACAATGGTCAGGCCGTTTCT+GGG + chr4.4:396534-396553 MS.gene040126:CDS 50.0%
GAAAGCAGCGAAGAAGAGAG+CGG - chr4.4:396203-396222 None:intergenic 50.0%
GAAGAAGAGAGCGGTGAAGA+TGG - chr4.4:396194-396213 None:intergenic 50.0%
GTAAGTTGTAACCGTCGACC+AGG - chr4.4:396714-396733 None:intergenic 50.0%
TCTCTCTTCTGCAACGGCAT+AGG + chr4.4:396622-396641 MS.gene040126:CDS 50.0%
TTCCTAATCGCATACCCTCC+AGG + chr4.4:396220-396239 MS.gene040126:CDS 50.0%
! TACCTGGAGGGTATGCGATT+AGG - chr4.4:396225-396244 None:intergenic 50.0%
! TCTCCTAGGTATTGCTGCAC+TGG + chr4.4:396886-396905 MS.gene040126:CDS 50.0%
! TGAATAAGCAGTGGGCTTGC+AGG - chr4.4:396932-396951 None:intergenic 50.0%
!! GTGCTGCTTTCAACTCTCCT+AGG + chr4.4:396872-396891 MS.gene040126:CDS 50.0%
AAACGGCCTGACCATTGTGG+TGG - chr4.4:396532-396551 None:intergenic 55.0%
AACGGCCTGACCATTGTGGT+GGG - chr4.4:396531-396550 None:intergenic 55.0%
ACCTCACCTGCAACCCTACT+GGG - chr4.4:396813-396832 None:intergenic 55.0%
CAAGTCCCACCACAATGGTC+AGG + chr4.4:396523-396542 MS.gene040126:CDS 55.0%
CAATGGTCAGGCCGTTTCTG+GGG + chr4.4:396535-396554 MS.gene040126:CDS 55.0%
CACAATGGTCAGGCCGTTTC+TGG + chr4.4:396533-396552 MS.gene040126:CDS 55.0%
CAGAAACGGCCTGACCATTG+TGG - chr4.4:396535-396554 None:intergenic 55.0%
CTCTTCTGCAACGGCATAGG+CGG + chr4.4:396625-396644 MS.gene040126:CDS 55.0%
GGCGGATCTCTCTTCTGCAA+CGG + chr4.4:396616-396635 MS.gene040126:CDS 55.0%
GTTGGATTGCTGGCCACACT+GGG - chr4.4:396435-396454 None:intergenic 55.0%
TATGACGTCAGCCTGGTCGA+CGG + chr4.4:396700-396719 MS.gene040126:CDS 55.0%
! ACCATTGTGGTGGGACTTGG+AGG - chr4.4:396522-396541 None:intergenic 55.0%
! ACGTGACAAGAAGCACGTGG+TGG + chr4.4:396834-396853 MS.gene040126:CDS 55.0%
! CCATTGTGGTGGGACTTGGA+GGG - chr4.4:396521-396540 None:intergenic 55.0%
! CGTGACAAGAAGCACGTGGT+GGG + chr4.4:396835-396854 MS.gene040126:CDS 55.0%
! CTGACCATTGTGGTGGGACT+TGG - chr4.4:396525-396544 None:intergenic 55.0%
! GTCACGTGACAAGAAGCACG+TGG + chr4.4:396831-396850 MS.gene040126:CDS 55.0%
!! AGAATGGGTTTTCCGGCACC+GGG - chr4.4:396457-396476 None:intergenic 55.0%
!! CAGAATGGGTTTTCCGGCAC+CGG - chr4.4:396458-396477 None:intergenic 55.0%
AACTCAGCGAGTGTGGCTGG+TGG - chr4.4:396655-396674 None:intergenic 60.0%
ACAAGTGTCCCCACCCAGTG+TGG + chr4.4:396419-396438 MS.gene040126:CDS 60.0%
ACCTTCGACGCATCAGGCCA+CGG + chr4.4:396571-396590 MS.gene040126:CDS 60.0%
ACTCAGCGAGTGTGGCTGGT+GGG - chr4.4:396654-396673 None:intergenic 60.0%
ATTGCTGGCCACACTGGGTG+GGG - chr4.4:396430-396449 None:intergenic 60.0%
CCCTCCAAGTCCCACCACAA+TGG + chr4.4:396518-396537 MS.gene040126:CDS 60.0%
GACCTCACCTGCAACCCTAC+TGG - chr4.4:396814-396833 None:intergenic 60.0%
GATGTGCCCAGTAGGGTTGC+AGG + chr4.4:396804-396823 MS.gene040126:CDS 60.0%
GATTGCTGGCCACACTGGGT+GGG - chr4.4:396431-396450 None:intergenic 60.0%
GGAAAACCCATTCTGGCCCG+CGG + chr4.4:396463-396482 MS.gene040126:CDS 60.0%
GGTTGGATTGCTGGCCACAC+TGG - chr4.4:396436-396455 None:intergenic 60.0%
GTGAACTCAGCGAGTGTGGC+TGG - chr4.4:396658-396677 None:intergenic 60.0%
GTGTGGCCAGCAATCCAACC+CGG + chr4.4:396436-396455 MS.gene040126:CDS 60.0%
! AGTGGGCTTGCAGGTCTGTG+GGG - chr4.4:396923-396942 None:intergenic 60.0%
! CAGTGGGCTTGCAGGTCTGT+GGG - chr4.4:396924-396943 None:intergenic 60.0%
! CGGTGCCGGAAAACCCATTC+TGG + chr4.4:396456-396475 MS.gene040126:CDS 60.0%
!! GCGGGCCAGAATGGGTTTTC+CGG - chr4.4:396464-396483 None:intergenic 60.0%
AAACCCATTCTGGCCCGCGG+CGG + chr4.4:396466-396485 MS.gene040126:CDS 65.0%
AGCCGTGACGGCCCCAGAAA+CGG - chr4.4:396549-396568 None:intergenic 65.0%
CATTGCGCCACCGGAGACTG+CGG + chr4.4:396595-396614 MS.gene040126:CDS 65.0%
CCAGCAATCCAACCCGGTGC+CGG + chr4.4:396442-396461 MS.gene040126:CDS 65.0%
CTCAGCGAGTGTGGCTGGTG+GGG - chr4.4:396653-396672 None:intergenic 65.0%
GAGAGATCCGCCGCAGTCTC+CGG - chr4.4:396608-396627 None:intergenic 65.0%
GCCCAGTAGGGTTGCAGGTG+AGG + chr4.4:396809-396828 MS.gene040126:CDS 65.0%
GGATTGCTGGCCACACTGGG+TGG - chr4.4:396432-396451 None:intergenic 65.0%
GGCTGCACCTTCGACGCATC+AGG + chr4.4:396565-396584 MS.gene040126:CDS 65.0%
! CGTCGAAGGTGCAGCCGTGA+CGG - chr4.4:396561-396580 None:intergenic 65.0%
! GCAGTGGGCTTGCAGGTCTG+TGG - chr4.4:396925-396944 None:intergenic 65.0%
!! TGGGTTTTCCGGCACCGGGT+TGG - chr4.4:396453-396472 None:intergenic 65.0%
AAGCCGCCGCGGGCCAGAAT+GGG - chr4.4:396472-396491 None:intergenic 70.0%
AGATCCGCCGCAGTCTCCGG+TGG - chr4.4:396605-396624 None:intergenic 70.0%
CGCAGTCTCCGGTGGCGCAA+TGG - chr4.4:396597-396616 None:intergenic 70.0%
GCCGTGGCCTGATGCGTCGA+AGG - chr4.4:396575-396594 None:intergenic 70.0%
GTGGGAGGTTGAAGCCGCCG+CGG - chr4.4:396483-396502 None:intergenic 70.0%
TGGGAGGTTGAAGCCGCCGC+GGG - chr4.4:396482-396501 None:intergenic 70.0%
! CCGGCACCGGGTTGGATTGC+TGG - chr4.4:396445-396464 None:intergenic 70.0%
!! GGCCGTTTCTGGGGCCGTCA+CGG + chr4.4:396544-396563 MS.gene040126:CDS 70.0%
CTCCGGTGGCGCAATGGCCG+TGG - chr4.4:396591-396610 None:intergenic 75.0%
GAAGCCGCCGCGGGCCAGAA+TGG - chr4.4:396473-396492 None:intergenic 75.0%
GGCCACGGCCATTGCGCCAC+CGG + chr4.4:396586-396605 MS.gene040126:CDS 75.0%
TGCGCCACCGGAGACTGCGG+CGG + chr4.4:396598-396617 MS.gene040126:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 396181 397071 396181 ID=MS.gene040126
chr4.4 mRNA 396181 397071 396181 ID=MS.gene040126.t1;Parent=MS.gene040126
chr4.4 exon 396181 396241 396181 ID=MS.gene040126.t1.exon1;Parent=MS.gene040126.t1
chr4.4 CDS 396181 396241 396181 ID=cds.MS.gene040126.t1;Parent=MS.gene040126.t1
chr4.4 exon 396380 397071 396380 ID=MS.gene040126.t1.exon2;Parent=MS.gene040126.t1
chr4.4 CDS 396380 397071 396380 ID=cds.MS.gene040126.t1;Parent=MS.gene040126.t1
Gene Sequence

>MS.gene040126

ATGGCTGCTTCCATCTTCACCGCTCTCTTCTTCGCTGCTTTCCTAATCGCATACCCTCCAGGTATTTTATTATTATTTCCTCCTTCTCTTCTCTTCTCTCTCTCTCACACACACAGAGAAAGCGGTTTGTGAGAGATATATAGTACATTTATTACACCGTTACTTAATTTGTTGTTTCTTGTTTGATTTTAACAAACAGTAGTGAAGGTATCAGCAGCAGCATCAGTAGTATTTTACAACAAGTGTCCCCACCCAGTGTGGCCAGCAATCCAACCCGGTGCCGGAAAACCCATTCTGGCCCGCGGCGGCTTCAACCTCCCACCAAACAAAGCTTATTCCCTCCAAGTCCCACCACAATGGTCAGGCCGTTTCTGGGGCCGTCACGGCTGCACCTTCGACGCATCAGGCCACGGCCATTGCGCCACCGGAGACTGCGGCGGATCTCTCTTCTGCAACGGCATAGGCGGAACCCCACCAGCCACACTCGCTGAGTTCACATTTGGGAAAGATCTAGATTTCTATGACGTCAGCCTGGTCGACGGTTACAACTTACCGATTTCCATTACACCATTCAAGGGATCTGGAAAATGTAGCTATGCTGGCTGTGTGAGTGATCTTAACTTGATGTGCCCAGTAGGGTTGCAGGTGAGGTCACGTGACAAGAAGCACGTGGTGGGTTGTAGAAGTGCTTGTGCTGCTTTCAACTCTCCTAGGTATTGCTGCACTGGTAACTTTGGTACCCCACAGACCTGCAAGCCCACTGCTTATTCAAGAATCTTCAAAACTGCTTGCCCAAAGGCTTACTCTTATGCTTATGATGACCCTACCAGTATTGCTACTTGCACCAATGCTAGTTACATGATCACTTTCTGCCCTCGTCACCGCCGTTGA
Protein sequence

>MS.gene040126.t1

MAASIFTALFFAAFLIAYPPVVKVSAAASVVFYNKCPHPVWPAIQPGAGKPILARGGFNLPPNKAYSLQVPPQWSGRFWGRHGCTFDASGHGHCATGDCGGSLFCNGIGGTPPATLAEFTFGKDLDFYDVSLVDGYNLPISITPFKGSGKCSYAGCVSDLNLMCPVGLQVRSRDKKHVVGCRSACAAFNSPRYCCTGNFGTPQTCKPTAYSRIFKTACPKAYSYAYDDPTSIATCTNASYMITFCPRHRR