AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42360.t1 MTR_3g111620 95.600 250 11 0 1 250 1 250 3.41e-169 467
MS.gene42360.t1 MTR_1g021945 78.788 231 47 2 21 250 20 249 4.26e-129 365
MS.gene42360.t1 MTR_8g036215 56.410 234 97 3 13 245 8 237 2.83e-91 269
MS.gene42360.t1 MTR_6g079580 53.333 240 103 4 13 246 4 240 1.03e-88 263
MS.gene42360.t1 MTR_8g107140 57.407 216 87 3 33 246 33 245 4.88e-88 261
MS.gene42360.t1 MTR_4g073720 54.357 241 102 5 7 245 5 239 2.88e-86 260
MS.gene42360.t1 MTR_5g022350 52.734 256 106 6 1 250 1 247 1.40e-85 257
MS.gene42360.t1 MTR_8g075510 55.466 247 98 7 6 249 10 247 5.62e-85 253
MS.gene42360.t1 MTR_8g037890 52.569 253 113 6 1 249 1 250 5.17e-84 253
MS.gene42360.t1 MTR_8g075510 55.556 243 96 7 6 245 10 243 4.10e-83 251
MS.gene42360.t1 MTR_8g075550 55.895 229 92 5 29 249 30 257 6.78e-80 243
MS.gene42360.t1 MTR_1g025420 52.632 247 108 8 10 249 7 251 7.26e-78 238
MS.gene42360.t1 MTR_3g114030 55.310 226 95 6 27 247 7 231 9.83e-78 234
MS.gene42360.t1 MTR_1g025420 52.697 241 107 7 10 245 7 245 1.51e-77 235
MS.gene42360.t1 MTR_8g056820 50.407 246 116 5 9 250 8 251 3.48e-77 234
MS.gene42360.t1 MTR_5g022310 51.837 245 108 6 13 250 14 255 1.64e-76 232
MS.gene42360.t1 MTR_1g062590 51.899 237 109 5 13 246 8 242 2.72e-76 231
MS.gene42360.t1 MTR_5g022310 51.406 249 111 6 9 250 10 255 3.20e-76 233
MS.gene42360.t1 MTR_5g059200 48.988 247 116 4 1 245 1 239 3.52e-76 232
MS.gene42360.t1 MTR_7g102380 52.321 237 106 6 13 246 9 241 6.23e-76 230
MS.gene42360.t1 MTR_1g062660 49.590 244 118 5 7 247 2 243 4.10e-75 228
MS.gene42360.t1 MTR_1g062350 50.628 239 113 5 13 248 8 244 1.78e-74 226
MS.gene42360.t1 MTR_1g062340 51.064 235 110 5 17 248 12 244 7.12e-74 225
MS.gene42360.t1 MTR_4g073730 51.667 240 110 5 12 246 10 248 6.15e-73 224
MS.gene42360.t1 MTR_3g068015 48.214 224 113 3 25 246 26 248 6.60e-73 223
MS.gene42360.t1 MTR_1g062370 49.372 239 116 5 13 248 8 244 7.00e-73 223
MS.gene42360.t1 MTR_7g062610 47.556 225 116 2 23 246 19 242 8.15e-73 223
MS.gene42360.t1 MTR_4g073730 51.667 240 110 5 12 246 10 248 1.38e-72 224
MS.gene42360.t1 MTR_1g062630 50.420 238 113 5 13 247 8 243 3.47e-72 221
MS.gene42360.t1 MTR_2g067980 48.387 248 120 6 9 250 6 251 2.59e-71 236
MS.gene42360.t1 MTR_2g069660 49.580 238 116 4 12 246 7 243 2.61e-70 216
MS.gene42360.t1 MTR_6g009480 42.857 252 138 4 1 246 1 252 1.54e-69 215
MS.gene42360.t1 MTR_2g068030 49.776 223 102 7 29 245 25 243 3.08e-69 214
MS.gene42360.t1 MTR_2g068655 45.815 227 117 6 24 246 28 252 2.57e-61 195
MS.gene42360.t1 MTR_1g062690 44.958 238 104 4 13 248 8 220 3.95e-61 192
MS.gene42360.t1 MTR_4g063630 39.831 236 136 5 15 245 17 251 4.35e-56 180
MS.gene42360.t1 MTR_5g010635 44.292 219 102 7 29 246 26 225 5.69e-52 169
MS.gene42360.t1 MTR_5g010640 39.370 254 128 9 1 246 1 236 5.61e-51 166
MS.gene42360.t1 MTR_8g096900 40.084 237 119 8 12 246 33 248 3.41e-48 159
MS.gene42360.t1 MTR_8g089020 46.584 161 64 3 81 241 21 159 3.09e-44 147
MS.gene42360.t1 MTR_2g067990 43.363 226 92 10 29 248 29 224 4.14e-44 159
MS.gene42360.t1 MTR_5g022350 50.000 162 70 5 1 156 66 222 1.22e-43 147
MS.gene42360.t1 MTR_2g067970 44.149 188 94 8 69 249 2 185 5.99e-40 147
MS.gene42360.t1 MTR_5g023850 36.437 247 112 9 12 250 11 220 4.37e-39 135
MS.gene42360.t1 MTR_2g063150 32.500 240 150 5 12 242 12 248 5.50e-39 136
MS.gene42360.t1 MTR_1g062390 31.621 253 152 6 6 250 3 242 2.24e-30 113
MS.gene42360.t1 MTR_8g096910 43.382 136 66 4 12 146 9 134 4.55e-28 105
MS.gene42360.t1 MTR_7g076360 33.484 221 81 5 24 242 5 161 1.79e-27 103
MS.gene42360.t1 MTR_8g096920 45.185 135 63 4 14 146 6 131 3.64e-26 100
MS.gene42360.t1 MTR_3g081550 44.792 96 50 3 109 202 15 109 9.01e-13 63.5
MS.gene42360.t1 MTR_1g062640 35.374 147 85 6 22 166 8 146 1.10e-12 64.3
MS.gene42360.t1 MTR_5g068670 46.753 77 37 3 64 139 12 85 9.79e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42360.t1 AT1G75030 65.690 239 79 3 13 249 9 246 1.86e-112 323
MS.gene42360.t1 AT1G75050 66.807 238 73 4 14 247 9 244 1.61e-111 321
MS.gene42360.t1 AT1G75040 60.581 241 91 4 6 246 3 239 1.31e-103 300
MS.gene42360.t1 AT1G19320 60.241 249 92 5 1 245 1 246 1.15e-96 283
MS.gene42360.t1 AT1G73620 56.444 225 93 3 24 246 39 260 6.80e-89 264
MS.gene42360.t1 AT1G18250 55.556 225 95 3 24 246 18 239 6.21e-88 261
MS.gene42360.t1 AT4G38660 55.738 244 98 6 9 250 14 249 1.99e-87 263
MS.gene42360.t1 AT1G18250 54.185 227 99 3 22 246 17 240 2.74e-87 259
MS.gene42360.t1 AT4G24180 55.870 247 96 7 9 247 11 252 8.82e-86 256
MS.gene42360.t1 AT4G24180 55.600 250 98 7 9 250 11 255 1.38e-85 257
MS.gene42360.t1 AT4G24180 54.980 251 97 7 9 247 11 257 2.09e-85 255
MS.gene42360.t1 AT4G24180 54.724 254 99 7 9 250 11 260 3.01e-85 256
MS.gene42360.t1 AT4G38660 57.269 227 89 5 26 250 7 227 9.32e-85 256
MS.gene42360.t1 AT1G77700 51.899 237 99 4 16 245 43 271 6.71e-83 251
MS.gene42360.t1 AT1G77700 51.899 237 99 4 16 245 43 271 2.79e-82 249
MS.gene42360.t1 AT5G24620 52.610 249 110 4 6 246 3 251 1.10e-81 251
MS.gene42360.t1 AT5G24620 52.610 249 110 4 6 246 58 306 2.09e-81 252
MS.gene42360.t1 AT5G24620 52.610 249 110 4 6 246 79 327 2.88e-81 252
MS.gene42360.t1 AT1G77700 53.394 221 95 3 25 245 87 299 4.69e-81 247
MS.gene42360.t1 AT1G75800 53.252 246 106 7 12 250 9 252 2.97e-78 239
MS.gene42360.t1 AT2G17860 53.469 245 105 6 13 249 9 252 1.02e-77 235
MS.gene42360.t1 AT4G36010 54.506 233 96 5 24 247 20 251 6.99e-77 235
MS.gene42360.t1 AT4G36010 54.506 233 96 5 24 247 20 251 6.99e-77 235
MS.gene42360.t1 AT4G24180 54.978 231 91 7 9 231 11 236 1.28e-76 235
MS.gene42360.t1 AT4G24180 54.043 235 92 7 9 231 11 241 1.61e-76 235
MS.gene42360.t1 AT1G20030 53.540 226 97 6 29 247 5 229 9.34e-74 227
MS.gene42360.t1 AT1G20030 53.540 226 97 6 29 247 22 246 1.15e-73 227
MS.gene42360.t1 AT5G40020 44.578 249 136 2 1 247 1 249 1.84e-73 224
MS.gene42360.t1 AT4G38670 50.000 244 110 8 14 246 5 247 3.10e-70 217
MS.gene42360.t1 AT4G38670 50.000 244 110 8 14 246 5 247 3.10e-70 217
MS.gene42360.t1 AT4G38670 51.786 224 101 7 29 246 9 231 2.12e-68 212
MS.gene42360.t1 AT5G02140 45.607 239 127 3 13 249 7 244 9.79e-68 211
MS.gene42360.t1 AT5G02140 45.763 236 125 3 13 246 7 241 1.20e-67 209
MS.gene42360.t1 AT5G38280 46.559 247 121 8 10 246 5 250 2.27e-63 209
MS.gene42360.t1 AT5G38280 46.559 247 121 8 10 246 5 250 3.72e-63 209
MS.gene42360.t1 AT2G28790 42.400 250 137 6 1 245 1 248 2.90e-59 188
MS.gene42360.t1 AT2G28790 42.400 250 137 6 1 245 26 273 3.42e-59 189
MS.gene42360.t1 AT4G11650 42.437 238 116 7 13 249 9 226 6.38e-56 179
MS.gene42360.t1 AT4G18253 39.837 246 135 6 7 247 2 239 1.90e-54 176
MS.gene42360.t1 AT4G36000 57.333 150 55 4 102 250 1 142 1.14e-51 167
MS.gene42360.t1 AT4G36000 57.333 150 55 4 102 250 1 142 1.14e-51 167
MS.gene42360.t1 AT1G70250 41.126 231 121 7 29 250 147 371 3.84e-46 164
MS.gene42360.t1 AT4G18250 41.152 243 131 8 11 248 39 274 4.93e-46 163
MS.gene42360.t1 AT5G24620 54.167 120 49 2 133 246 15 134 8.23e-35 127
MS.gene42360.t1 AT2G24810 60.256 78 30 1 168 245 116 192 1.93e-25 99.4

Find 85 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGCTGGTCTGGACGTTTC+TGG 0.041189 3.1:+89200416 MS.gene42360:CDS
ATCTGGCCGGGAACACTTTC+CGG 0.214212 3.1:+89200319 MS.gene42360:CDS
CGCCGCACACGCACTCTTAC+AGG 0.259177 3.1:-89200743 None:intergenic
TCAAGCACTTGCACATGTTC+TGG 0.269659 3.1:+89200892 MS.gene42360:CDS
CGGAAAGGTGTTTGAGTTAG+TGG 0.339140 3.1:-89200126 None:intergenic
CTTTGCAGGTAATGTAGCTT+CGG 0.347218 3.1:+89200258 MS.gene42360:intron
AGGCTGGTCTGGACGTTTCT+GGG 0.353237 3.1:+89200417 MS.gene42360:CDS
ACTGCTCCAGCAGGCTGGTC+TGG 0.372462 3.1:+89200406 MS.gene42360:CDS
TCATTGTCCAGCGGAGTTGC+AGG 0.378054 3.1:+89200693 MS.gene42360:CDS
TTTGCAGGTAATGTAGCTTC+GGG 0.380079 3.1:+89200259 MS.gene42360:intron
AGACTTCTATGACGTGAGTT+TGG 0.392352 3.1:+89200582 MS.gene42360:CDS
ACTTCGACGGTGCTGGTAAC+GGG 0.395193 3.1:+89200455 MS.gene42360:CDS
AAGTAGTTCAGCACCGTTGC+CGG 0.396672 3.1:-89200338 None:intergenic
CCGTTGCCGGAAAGTGTTCC+CGG 0.398738 3.1:-89200325 None:intergenic
TTTACGATCGGAAGCGGGAA+TGG 0.433505 3.1:+89200553 MS.gene42360:CDS
GTAAACTCCGCCAACGTCAC+TGG 0.437210 3.1:-89200535 None:intergenic
GATGGTTACAATGTGGGGAT+AGG 0.440861 3.1:+89200607 MS.gene42360:CDS
CTGACAATCACCTGTTCCTC+TGG 0.440961 3.1:-89200641 None:intergenic
ACCGTTGCATTTCAATCCTC+CGG 0.443760 3.1:-89200500 None:intergenic
ATACAAGCAACCAGAGGAAC+AGG 0.461854 3.1:+89200631 MS.gene42360:CDS
ACCGTTGTAACAACACAATC+TGG 0.462686 3.1:+89200302 MS.gene42360:CDS
TTTGAATTCACTGCTCCAGC+AGG 0.465707 3.1:+89200397 MS.gene42360:CDS
GTGCTGAACTACTTGGTGAC+GGG 0.474840 3.1:+89200347 MS.gene42360:CDS
GAGTTGCAGGTAACAGAGGA+AGG 0.484221 3.1:+89200706 MS.gene42360:CDS
GGCTGCAACTTCGACGGTGC+TGG 0.484415 3.1:+89200448 MS.gene42360:CDS
AAACGTCCAGACCAGCCTGC+TGG 0.486502 3.1:-89200412 None:intergenic
ATGGTTACAATGTGGGGATA+GGG 0.488169 3.1:+89200608 MS.gene42360:CDS
AACTTCGACGGTGCTGGTAA+CGG 0.498037 3.1:+89200454 MS.gene42360:CDS
ATCCTTCTGCTGCTTCTACT+AGG 0.498370 3.1:+89200155 MS.gene42360:CDS
GGACGTTTCTGGGGTAGAAC+CGG 0.500730 3.1:+89200427 MS.gene42360:CDS
TCTGTTACCTGCAACTCCGC+TGG 0.503530 3.1:-89200700 None:intergenic
TTGGTGGATGGTTACAATGT+GGG 0.504388 3.1:+89200601 MS.gene42360:CDS
TGCTCCTCCAGTGACGTTGG+CGG 0.507952 3.1:+89200528 MS.gene42360:CDS
AATTCACTGCTCCAGCAGGC+TGG 0.511094 3.1:+89200401 MS.gene42360:CDS
CCGGGAACACTTTCCGGCAA+CGG 0.521195 3.1:+89200325 MS.gene42360:CDS
TTGTAACAACACAATCTGGC+CGG 0.526132 3.1:+89200306 MS.gene42360:CDS
GCGGAGTTTACGATCGGAAG+CGG 0.539163 3.1:+89200547 MS.gene42360:CDS
GGCAACGGTGCTGAACTACT+TGG 0.539389 3.1:+89200340 MS.gene42360:CDS
GTGAATTCAAAAGATGATCC+TGG 0.540487 3.1:-89200385 None:intergenic
CGGAGTTTACGATCGGAAGC+GGG 0.541171 3.1:+89200548 MS.gene42360:CDS
GCCAGATTGTGTTGTTACAA+CGG 0.555322 3.1:-89200303 None:intergenic
ACAGGTGATTGTCAGTATGC+AGG 0.556822 3.1:+89200649 MS.gene42360:CDS
TGTAACAACACAATCTGGCC+GGG 0.563673 3.1:+89200307 MS.gene42360:CDS
AACATCTCTGAGTACTGTGT+TGG 0.565049 3.1:-89200823 None:intergenic
CTTCTATGACGTGAGTTTGG+TGG 0.567400 3.1:+89200585 MS.gene42360:CDS
GGATTGAAATGCAACGGTGG+AGG 0.575754 3.1:+89200505 MS.gene42360:CDS
TGGTGGATGGTTACAATGTG+GGG 0.575782 3.1:+89200602 MS.gene42360:CDS
CTGTGGACTCGAATGTTCAC+CGG 0.577729 3.1:-89200791 None:intergenic
GGTAACGGGAATTGCATCAC+AGG 0.579922 3.1:+89200469 MS.gene42360:CDS
GTTGCAGGTAACAGAGGAAG+GGG 0.591850 3.1:+89200708 MS.gene42360:CDS
AGCAGCAGAAGGATGATGAA+CGG 0.593386 3.1:-89200146 None:intergenic
TTGAATGTGGTATGGCTAGG+AGG 0.593673 3.1:-89200950 None:intergenic
GGTGCTGAACTACTTGGTGA+CGG 0.595292 3.1:+89200346 MS.gene42360:CDS
TATGGCTAGGAGGAAGCAGT+TGG 0.600723 3.1:-89200940 None:intergenic
GGTTGTGTTGCGGATTTGAA+CGG 0.603815 3.1:+89200670 MS.gene42360:CDS
AGCACCGTCGAAGTTGCAGC+CGG 0.605393 3.1:-89200446 None:intergenic
ACCGGTGCAACAAAACTCCT+CGG 0.606971 3.1:-89200773 None:intergenic
AGCGGAGTTGCAGGTAACAG+AGG 0.608898 3.1:+89200702 MS.gene42360:CDS
TGTGTTGGACTGCAACTCTG+TGG 0.610848 3.1:-89200808 None:intergenic
AGGTGCTCCTCCAGTGACGT+TGG 0.611186 3.1:+89200525 MS.gene42360:CDS
TGCTGAACTACTTGGTGACG+GGG 0.611875 3.1:+89200348 MS.gene42360:CDS
ACCGGAGGATTGAAATGCAA+CGG 0.612318 3.1:+89200499 MS.gene42360:CDS
CACCTAGTAGAAGCAGCAGA+AGG 0.613519 3.1:-89200157 None:intergenic
TATGACGTGAGTTTGGTGGA+TGG 0.616012 3.1:+89200589 MS.gene42360:CDS
GGCTGGTCTGGACGTTTCTG+GGG 0.623771 3.1:+89200418 MS.gene42360:CDS
AGAACCGGCTGCAACTTCGA+CGG 0.626510 3.1:+89200442 MS.gene42360:CDS
TTTGGTGGATGGTTACAATG+TGG 0.629478 3.1:+89200600 MS.gene42360:CDS
ACGTTGGCGGAGTTTACGAT+CGG 0.633255 3.1:+89200541 MS.gene42360:CDS
TGCATCACAGGAGACTGCAC+CGG 0.636449 3.1:+89200481 MS.gene42360:CDS
TGCCTGTAAGAGTGCGTGTG+CGG 0.637848 3.1:+89200741 MS.gene42360:CDS
ATCACAGGAGACTGCACCGG+AGG 0.642936 3.1:+89200484 MS.gene42360:CDS
TAAGCATAACTGTATGCAGA+GGG 0.644508 3.1:-89200859 None:intergenic
AGTTGCAGGTAACAGAGGAA+GGG 0.644950 3.1:+89200707 MS.gene42360:CDS
CAGAAGGATGATGAACGGAA+AGG 0.647623 3.1:-89200141 None:intergenic
AACTCCGCCAACGTCACTGG+AGG 0.649133 3.1:-89200532 None:intergenic
TTGCAGGTAATGTAGCTTCG+GGG 0.669955 3.1:+89200260 MS.gene42360:intron
TCAGTATGCAGGTTGTGTTG+CGG 0.671374 3.1:+89200660 MS.gene42360:CDS
GCTGAACTACTTGGTGACGG+GGG 0.674143 3.1:+89200349 MS.gene42360:CDS
TTTGAACGGTCATTGTCCAG+CGG 0.681723 3.1:+89200684 MS.gene42360:CDS
GTGTGCGGCGTTTAATACCG+AGG 0.685026 3.1:+89200756 MS.gene42360:CDS
TTGCAGGTAACAGAGGAAGG+GGG 0.708103 3.1:+89200709 MS.gene42360:CDS
GGAGGATTGAAATGCAACGG+TGG 0.716120 3.1:+89200502 MS.gene42360:CDS
ATAAGCATAACTGTATGCAG+AGG 0.719851 3.1:-89200860 None:intergenic
ATAGGGATACAAGCAACCAG+AGG 0.737024 3.1:+89200625 MS.gene42360:CDS
AATTCAAAAGATGATCCTGG+CGG 0.756655 3.1:-89200382 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTACTTTACAAAAGACA+TGG - chr3.1:89200212-89200231 None:intergenic 20.0%
!! ACAAAAAGTGATAAGATAAT+CGG - chr3.1:89200920-89200939 None:intergenic 20.0%
!!! TTGTTTTTAAACTGCTAGTA+TGG + chr3.1:89200231-89200250 MS.gene42360:intron 25.0%
AATTCAAAAGATGATCCTGG+CGG - chr3.1:89200385-89200404 None:intergenic 35.0%
ATAAGCATAACTGTATGCAG+AGG - chr3.1:89200863-89200882 None:intergenic 35.0%
GTGAATTCAAAAGATGATCC+TGG - chr3.1:89200388-89200407 None:intergenic 35.0%
TAAGCATAACTGTATGCAGA+GGG - chr3.1:89200862-89200881 None:intergenic 35.0%
! GTTGTTACAACGGTTTTCTA+TGG - chr3.1:89200296-89200315 None:intergenic 35.0%
ACCGTTGTAACAACACAATC+TGG + chr3.1:89200302-89200321 MS.gene42360:CDS 40.0%
AGACTTCTATGACGTGAGTT+TGG + chr3.1:89200582-89200601 MS.gene42360:CDS 40.0%
ATGGTTACAATGTGGGGATA+GGG + chr3.1:89200608-89200627 MS.gene42360:CDS 40.0%
CTTTGCAGGTAATGTAGCTT+CGG + chr3.1:89200258-89200277 MS.gene42360:intron 40.0%
TTGTAACAACACAATCTGGC+CGG + chr3.1:89200306-89200325 MS.gene42360:CDS 40.0%
TTTGCAGGTAATGTAGCTTC+GGG + chr3.1:89200259-89200278 MS.gene42360:intron 40.0%
! AACATCTCTGAGTACTGTGT+TGG - chr3.1:89200826-89200845 None:intergenic 40.0%
! GCCAGATTGTGTTGTTACAA+CGG - chr3.1:89200306-89200325 None:intergenic 40.0%
! GCTAGTATGGTTTTCTTTGC+AGG + chr3.1:89200244-89200263 MS.gene42360:intron 40.0%
! TTGGTGGATGGTTACAATGT+GGG + chr3.1:89200601-89200620 MS.gene42360:CDS 40.0%
! TTTGGTGGATGGTTACAATG+TGG + chr3.1:89200600-89200619 MS.gene42360:CDS 40.0%
ACAGGTGATTGTCAGTATGC+AGG + chr3.1:89200649-89200668 MS.gene42360:CDS 45.0%
ACCGGAGGATTGAAATGCAA+CGG + chr3.1:89200499-89200518 MS.gene42360:CDS 45.0%
ACCGTTGCATTTCAATCCTC+CGG - chr3.1:89200503-89200522 None:intergenic 45.0%
AGCAGCAGAAGGATGATGAA+CGG - chr3.1:89200149-89200168 None:intergenic 45.0%
AGTTGCAGGTAACAGAGGAA+GGG + chr3.1:89200707-89200726 MS.gene42360:CDS 45.0%
ATACAAGCAACCAGAGGAAC+AGG + chr3.1:89200631-89200650 MS.gene42360:CDS 45.0%
ATAGGGATACAAGCAACCAG+AGG + chr3.1:89200625-89200644 MS.gene42360:CDS 45.0%
CAGAAGGATGATGAACGGAA+AGG - chr3.1:89200144-89200163 None:intergenic 45.0%
CTTCTATGACGTGAGTTTGG+TGG + chr3.1:89200585-89200604 MS.gene42360:CDS 45.0%
GATGGTTACAATGTGGGGAT+AGG + chr3.1:89200607-89200626 MS.gene42360:CDS 45.0%
GGTTGTGTTGCGGATTTGAA+CGG + chr3.1:89200670-89200689 MS.gene42360:CDS 45.0%
TCAAGCACTTGCACATGTTC+TGG + chr3.1:89200892-89200911 MS.gene42360:CDS 45.0%
TCAGTATGCAGGTTGTGTTG+CGG + chr3.1:89200660-89200679 MS.gene42360:CDS 45.0%
TGTAACAACACAATCTGGCC+GGG + chr3.1:89200307-89200326 MS.gene42360:CDS 45.0%
TTGCAGGTAATGTAGCTTCG+GGG + chr3.1:89200260-89200279 MS.gene42360:intron 45.0%
TTTGAACGGTCATTGTCCAG+CGG + chr3.1:89200684-89200703 MS.gene42360:CDS 45.0%
TTTGAATTCACTGCTCCAGC+AGG + chr3.1:89200397-89200416 MS.gene42360:CDS 45.0%
! ATCCTTCTGCTGCTTCTACT+AGG + chr3.1:89200155-89200174 MS.gene42360:CDS 45.0%
! TATGACGTGAGTTTGGTGGA+TGG + chr3.1:89200589-89200608 MS.gene42360:CDS 45.0%
! TGGTGGATGGTTACAATGTG+GGG + chr3.1:89200602-89200621 MS.gene42360:CDS 45.0%
!! CGGAAAGGTGTTTGAGTTAG+TGG - chr3.1:89200129-89200148 None:intergenic 45.0%
AAGTAGTTCAGCACCGTTGC+CGG - chr3.1:89200341-89200360 None:intergenic 50.0%
ACCGGTGCAACAAAACTCCT+CGG - chr3.1:89200776-89200795 None:intergenic 50.0%
ACGTTGGCGGAGTTTACGAT+CGG + chr3.1:89200541-89200560 MS.gene42360:CDS 50.0%
CACCTAGTAGAAGCAGCAGA+AGG - chr3.1:89200160-89200179 None:intergenic 50.0%
CTGACAATCACCTGTTCCTC+TGG - chr3.1:89200644-89200663 None:intergenic 50.0%
CTGTGGACTCGAATGTTCAC+CGG - chr3.1:89200794-89200813 None:intergenic 50.0%
GAGTTGCAGGTAACAGAGGA+AGG + chr3.1:89200706-89200725 MS.gene42360:CDS 50.0%
GGAGGATTGAAATGCAACGG+TGG + chr3.1:89200502-89200521 MS.gene42360:CDS 50.0%
GGATTGAAATGCAACGGTGG+AGG + chr3.1:89200505-89200524 MS.gene42360:CDS 50.0%
GTTGCAGGTAACAGAGGAAG+GGG + chr3.1:89200708-89200727 MS.gene42360:CDS 50.0%
TGTGTTGGACTGCAACTCTG+TGG - chr3.1:89200811-89200830 None:intergenic 50.0%
TTGCAGGTAACAGAGGAAGG+GGG + chr3.1:89200709-89200728 MS.gene42360:CDS 50.0%
TTTACGATCGGAAGCGGGAA+TGG + chr3.1:89200553-89200572 MS.gene42360:CDS 50.0%
! GGTGCTGAACTACTTGGTGA+CGG + chr3.1:89200346-89200365 MS.gene42360:CDS 50.0%
! GTGCTGAACTACTTGGTGAC+GGG + chr3.1:89200347-89200366 MS.gene42360:CDS 50.0%
!! AACTTCGACGGTGCTGGTAA+CGG + chr3.1:89200454-89200473 MS.gene42360:CDS 50.0%
!! GGTAACGGGAATTGCATCAC+AGG + chr3.1:89200469-89200488 MS.gene42360:CDS 50.0%
!! TGCTGAACTACTTGGTGACG+GGG + chr3.1:89200348-89200367 MS.gene42360:CDS 50.0%
!!! ACCGAGGAGTTTTGTTGCAC+CGG + chr3.1:89200772-89200791 MS.gene42360:CDS 50.0%
AATTCACTGCTCCAGCAGGC+TGG + chr3.1:89200401-89200420 MS.gene42360:CDS 55.0%
AGAACCGGCTGCAACTTCGA+CGG + chr3.1:89200442-89200461 MS.gene42360:CDS 55.0%
AGCGGAGTTGCAGGTAACAG+AGG + chr3.1:89200702-89200721 MS.gene42360:CDS 55.0%
ATCTGGCCGGGAACACTTTC+CGG + chr3.1:89200319-89200338 MS.gene42360:CDS 55.0%
CGGAGTTTACGATCGGAAGC+GGG + chr3.1:89200548-89200567 MS.gene42360:CDS 55.0%
GCGGAGTTTACGATCGGAAG+CGG + chr3.1:89200547-89200566 MS.gene42360:CDS 55.0%
GGTAACAGAGGAAGGGGGTT+CGG + chr3.1:89200714-89200733 MS.gene42360:CDS 55.0%
GTAAACTCCGCCAACGTCAC+TGG - chr3.1:89200538-89200557 None:intergenic 55.0%
GTGTGCGGCGTTTAATACCG+AGG + chr3.1:89200756-89200775 MS.gene42360:CDS 55.0%
TCATTGTCCAGCGGAGTTGC+AGG + chr3.1:89200693-89200712 MS.gene42360:CDS 55.0%
TCTGTTACCTGCAACTCCGC+TGG - chr3.1:89200703-89200722 None:intergenic 55.0%
TGCATCACAGGAGACTGCAC+CGG + chr3.1:89200481-89200500 MS.gene42360:CDS 55.0%
! GGCAACGGTGCTGAACTACT+TGG + chr3.1:89200340-89200359 MS.gene42360:CDS 55.0%
! TGCCTGTAAGAGTGCGTGTG+CGG + chr3.1:89200741-89200760 MS.gene42360:CDS 55.0%
!! ACTTCGACGGTGCTGGTAAC+GGG + chr3.1:89200455-89200474 MS.gene42360:CDS 55.0%
!! AGGCTGGTCTGGACGTTTCT+GGG + chr3.1:89200417-89200436 MS.gene42360:CDS 55.0%
!! GCTGAACTACTTGGTGACGG+GGG + chr3.1:89200349-89200368 MS.gene42360:CDS 55.0%
!! GGACGTTTCTGGGGTAGAAC+CGG + chr3.1:89200427-89200446 MS.gene42360:CDS 55.0%
AAACGTCCAGACCAGCCTGC+TGG - chr3.1:89200415-89200434 None:intergenic 60.0%
AACAGAGGAAGGGGGTTCGG+TGG + chr3.1:89200717-89200736 MS.gene42360:CDS 60.0%
AACTCCGCCAACGTCACTGG+AGG - chr3.1:89200535-89200554 None:intergenic 60.0%
AGCACCGTCGAAGTTGCAGC+CGG - chr3.1:89200449-89200468 None:intergenic 60.0%
AGGTGCTCCTCCAGTGACGT+TGG + chr3.1:89200525-89200544 MS.gene42360:CDS 60.0%
ATCACAGGAGACTGCACCGG+AGG + chr3.1:89200484-89200503 MS.gene42360:CDS 60.0%
CCGGGAACACTTTCCGGCAA+CGG + chr3.1:89200325-89200344 MS.gene42360:CDS 60.0%
TGCTCCTCCAGTGACGTTGG+CGG + chr3.1:89200528-89200547 MS.gene42360:CDS 60.0%
!! CAGGCTGGTCTGGACGTTTC+TGG + chr3.1:89200416-89200435 MS.gene42360:CDS 60.0%
!! CCGTTGCCGGAAAGTGTTCC+CGG - chr3.1:89200328-89200347 None:intergenic 60.0%
!! GGCTGGTCTGGACGTTTCTG+GGG + chr3.1:89200418-89200437 MS.gene42360:CDS 60.0%
CGCCGCACACGCACTCTTAC+AGG - chr3.1:89200746-89200765 None:intergenic 65.0%
GGCTGCAACTTCGACGGTGC+TGG + chr3.1:89200448-89200467 MS.gene42360:CDS 65.0%
! ACTGCTCCAGCAGGCTGGTC+TGG + chr3.1:89200406-89200425 MS.gene42360:CDS 65.0%
! GGGGGATTTTCTCTGCCGCC+AGG + chr3.1:89200367-89200386 MS.gene42360:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 89200116 89200957 89200116 ID=MS.gene42360
chr3.1 mRNA 89200116 89200957 89200116 ID=MS.gene42360.t1;Parent=MS.gene42360
chr3.1 exon 89200116 89200176 89200116 ID=MS.gene42360.t1.exon1;Parent=MS.gene42360.t1
chr3.1 CDS 89200116 89200176 89200116 ID=cds.MS.gene42360.t1;Parent=MS.gene42360.t1
chr3.1 exon 89200266 89200957 89200266 ID=MS.gene42360.t1.exon2;Parent=MS.gene42360.t1
chr3.1 CDS 89200266 89200957 89200266 ID=cds.MS.gene42360.t1;Parent=MS.gene42360.t1
Gene Sequence

>MS.gene42360

ATGGCTCTTACCACTAACTCAAACACCTTTCCGTTCATCATCCTTCTGCTGCTTCTACTAGGTGAACAAGAAATTCAACACTGATTTTACGTTCCATGTCTTTTGTAAAGTAATTTTGTTTTTAAACTGCTAGTATGGTTTTCTTTGCAGGTAATGTAGCTTCGGGGACAATGTTTACCATAGAAAACCGTTGTAACAACACAATCTGGCCGGGAACACTTTCCGGCAACGGTGCTGAACTACTTGGTGACGGGGGATTTTCTCTGCCGCCAGGATCATCTTTTGAATTCACTGCTCCAGCAGGCTGGTCTGGACGTTTCTGGGGTAGAACCGGCTGCAACTTCGACGGTGCTGGTAACGGGAATTGCATCACAGGAGACTGCACCGGAGGATTGAAATGCAACGGTGGAGGTGCTCCTCCAGTGACGTTGGCGGAGTTTACGATCGGAAGCGGGAATGGAGATAAAGACTTCTATGACGTGAGTTTGGTGGATGGTTACAATGTGGGGATAGGGATACAAGCAACCAGAGGAACAGGTGATTGTCAGTATGCAGGTTGTGTTGCGGATTTGAACGGTCATTGTCCAGCGGAGTTGCAGGTAACAGAGGAAGGGGGTTCGGTGGTTGCCTGTAAGAGTGCGTGTGCGGCGTTTAATACCGAGGAGTTTTGTTGCACCGGTGAACATTCGAGTCCACAGAGTTGCAGTCCAACACAGTACTCAGAGATGTTCAAGACTGCGTGTCCCTCTGCATACAGTTATGCTTATGACGATGCTTCAAGCACTTGCACATGTTCTGGATCCGATTATCTTATCACTTTTTGTCCAACTGCTTCCTCCTAG
Protein sequence

>MS.gene42360.t1

MALTTNSNTFPFIILLLLLLGNVASGTMFTIENRCNNTIWPGTLSGNGAELLGDGGFSLPPGSSFEFTAPAGWSGRFWGRTGCNFDGAGNGNCITGDCTGGLKCNGGGAPPVTLAEFTIGSGNGDKDFYDVSLVDGYNVGIGIQATRGTGDCQYAGCVADLNGHCPAELQVTEEGGSVVACKSACAAFNTEEFCCTGEHSSPQSCSPTQYSEMFKTACPSAYSYAYDDASSTCTCSGSDYLITFCPTASS