AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072805

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072805.t1 MTR_5g010635 97.925 241 5 0 1 241 1 241 7.92e-178 488
MS.gene072805.t1 MTR_8g096900 63.348 221 78 2 6 225 30 248 1.68e-102 298
MS.gene072805.t1 MTR_5g010640 54.825 228 94 1 7 225 9 236 2.41e-90 266
MS.gene072805.t1 MTR_8g096910 46.018 226 71 4 1 225 1 176 6.37e-57 179
MS.gene072805.t1 MTR_8g096920 44.690 226 71 4 1 225 1 173 1.67e-55 176
MS.gene072805.t1 MTR_5g059200 47.511 221 96 8 19 224 24 239 1.09e-54 177
MS.gene072805.t1 MTR_1g021945 43.860 228 106 7 21 228 23 248 1.06e-52 171
MS.gene072805.t1 MTR_6g079580 42.083 240 115 8 6 225 5 240 1.20e-51 168
MS.gene072805.t1 MTR_4g073720 44.856 243 111 12 1 224 1 239 1.86e-50 168
MS.gene072805.t1 MTR_3g111620 43.636 220 102 8 26 225 29 246 5.72e-50 164
MS.gene072805.t1 MTR_8g075510 41.463 246 113 10 3 230 17 249 6.90e-50 164
MS.gene072805.t1 MTR_1g025420 39.271 247 125 8 1 224 1 245 1.82e-49 163
MS.gene072805.t1 MTR_1g025420 39.271 247 125 8 1 224 1 245 4.04e-49 164
MS.gene072805.t1 MTR_8g075510 41.667 240 109 10 3 224 17 243 4.84e-48 160
MS.gene072805.t1 MTR_8g107140 38.866 247 123 9 2 225 4 245 1.21e-47 158
MS.gene072805.t1 MTR_5g022350 40.078 257 120 14 3 239 14 256 2.68e-47 159
MS.gene072805.t1 MTR_5g022310 40.081 247 118 10 7 225 7 251 3.38e-47 157
MS.gene072805.t1 MTR_5g022310 40.081 247 118 10 7 225 7 251 4.41e-47 158
MS.gene072805.t1 MTR_8g037890 40.496 242 118 9 8 225 8 247 6.41e-47 158
MS.gene072805.t1 MTR_7g102380 41.423 239 116 10 9 225 5 241 1.05e-46 155
MS.gene072805.t1 MTR_2g067980 41.532 248 117 11 1 225 5 247 1.17e-46 166
MS.gene072805.t1 MTR_8g056820 39.506 243 121 9 7 225 7 247 1.24e-46 155
MS.gene072805.t1 MTR_3g114030 39.823 226 112 7 23 225 6 230 1.30e-46 155
MS.gene072805.t1 MTR_4g073730 39.200 250 120 9 1 226 8 249 1.60e-46 156
MS.gene072805.t1 MTR_4g073730 39.200 250 120 9 1 226 8 249 2.43e-46 157
MS.gene072805.t1 MTR_8g036215 38.174 241 125 9 4 224 1 237 4.69e-46 153
MS.gene072805.t1 MTR_2g069660 38.912 239 126 6 7 225 5 243 1.19e-45 152
MS.gene072805.t1 MTR_1g062590 38.525 244 126 9 4 225 1 242 5.65e-45 151
MS.gene072805.t1 MTR_1g062630 37.945 253 131 11 4 234 1 249 5.87e-45 151
MS.gene072805.t1 MTR_8g075550 40.175 229 108 8 23 224 27 253 1.14e-43 149
MS.gene072805.t1 MTR_2g068030 40.611 229 112 9 18 224 17 243 1.19e-43 148
MS.gene072805.t1 MTR_3g068015 41.553 219 105 9 28 225 32 248 2.68e-43 147
MS.gene072805.t1 MTR_1g062340 40.708 226 105 9 24 225 22 242 3.53e-43 146
MS.gene072805.t1 MTR_7g062610 37.652 247 127 9 1 225 1 242 1.98e-42 144
MS.gene072805.t1 MTR_1g062350 40.359 223 110 8 24 225 22 242 4.84e-41 140
MS.gene072805.t1 MTR_4g063630 37.447 235 121 9 14 224 19 251 7.10e-41 140
MS.gene072805.t1 MTR_2g068655 37.991 229 118 9 21 227 28 254 8.08e-41 142
MS.gene072805.t1 MTR_1g062370 39.910 223 111 8 24 225 22 242 9.42e-41 140
MS.gene072805.t1 MTR_6g009480 36.400 250 131 10 4 227 7 254 3.46e-40 139
MS.gene072805.t1 MTR_1g062660 38.462 234 121 8 13 225 11 242 6.43e-40 138
MS.gene072805.t1 MTR_2g067990 41.905 210 102 9 23 225 26 222 1.77e-35 134
MS.gene072805.t1 MTR_1g062690 35.294 221 100 8 24 225 22 218 7.46e-29 108
MS.gene072805.t1 MTR_2g067970 34.197 193 107 9 58 233 1 190 3.87e-23 98.2
MS.gene072805.t1 MTR_5g023850 33.333 168 97 5 9 161 11 178 7.54e-23 93.2
MS.gene072805.t1 MTR_8g089020 32.370 173 81 6 57 213 1 153 1.64e-21 87.8
MS.gene072805.t1 MTR_1g062390 27.848 237 150 8 6 225 6 238 4.76e-21 89.0
MS.gene072805.t1 MTR_5g022350 41.007 139 61 7 3 130 79 207 1.82e-20 86.7
MS.gene072805.t1 MTR_2g063150 28.326 233 144 10 1 213 10 239 5.52e-18 80.9
MS.gene072805.t1 MTR_7g076360 30.964 197 85 7 21 213 5 154 7.14e-18 78.6
MS.gene072805.t1 MTR_5g068670 50.000 64 30 2 68 130 25 87 1.74e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072805.t1 AT4G11650 73.778 225 57 2 1 225 1 223 1.59e-120 343
MS.gene072805.t1 AT1G75050 44.215 242 114 6 3 225 4 243 1.37e-58 186
MS.gene072805.t1 AT1G75040 42.623 244 116 7 1 225 1 239 1.05e-56 181
MS.gene072805.t1 AT1G75030 42.041 245 120 7 1 225 1 243 4.11e-54 174
MS.gene072805.t1 AT1G77700 43.590 234 112 7 6 224 43 271 1.32e-53 176
MS.gene072805.t1 AT1G77700 43.590 234 112 7 6 224 43 271 1.68e-53 175
MS.gene072805.t1 AT4G38660 45.798 238 109 10 5 225 11 245 9.39e-53 174
MS.gene072805.t1 AT1G77700 44.495 218 101 7 22 224 87 299 1.83e-52 174
MS.gene072805.t1 AT4G38670 39.754 244 122 9 6 225 5 247 3.62e-51 168
MS.gene072805.t1 AT4G38670 39.754 244 122 9 6 225 5 247 3.62e-51 168
MS.gene072805.t1 AT5G24620 41.434 251 114 10 3 225 6 251 5.08e-51 171
MS.gene072805.t1 AT5G24620 41.434 251 114 10 3 225 61 306 8.71e-51 172
MS.gene072805.t1 AT5G24620 41.434 251 114 10 3 225 82 327 1.12e-50 172
MS.gene072805.t1 AT1G19320 40.244 246 117 10 1 224 9 246 2.72e-49 162
MS.gene072805.t1 AT4G38660 45.778 225 102 10 18 225 2 223 3.56e-49 164
MS.gene072805.t1 AT1G73620 42.222 225 107 7 21 225 39 260 1.11e-48 161
MS.gene072805.t1 AT4G38670 40.789 228 110 9 22 225 5 231 4.71e-48 159
MS.gene072805.t1 AT1G75800 40.175 229 111 7 22 225 21 248 1.42e-47 160
MS.gene072805.t1 AT1G20030 40.708 226 108 7 25 225 4 228 2.12e-47 159
MS.gene072805.t1 AT1G18250 41.778 225 108 8 21 225 19 240 2.71e-47 157
MS.gene072805.t1 AT1G20030 40.708 226 108 7 25 225 21 245 3.52e-47 159
MS.gene072805.t1 AT1G18250 41.333 225 109 8 21 225 18 239 5.54e-47 156
MS.gene072805.t1 AT2G17860 40.083 242 116 9 11 225 10 249 1.55e-46 155
MS.gene072805.t1 AT4G36010 40.167 239 113 9 22 232 21 257 2.47e-45 154
MS.gene072805.t1 AT4G36010 40.167 239 113 9 22 232 21 257 2.47e-45 154
MS.gene072805.t1 AT4G24180 37.984 258 116 11 1 225 10 256 2.67e-43 147
MS.gene072805.t1 AT4G24180 37.984 258 116 11 1 225 10 256 1.28e-42 147
MS.gene072805.t1 AT4G24180 38.400 250 109 12 9 225 14 251 3.82e-42 144
MS.gene072805.t1 AT4G24180 38.400 250 109 12 9 225 14 251 1.14e-41 144
MS.gene072805.t1 AT5G38280 34.630 257 141 8 7 238 9 263 4.71e-40 146
MS.gene072805.t1 AT5G38280 34.630 257 141 8 7 238 9 263 5.98e-40 145
MS.gene072805.t1 AT5G02140 37.131 237 127 9 10 225 6 241 1.37e-37 133
MS.gene072805.t1 AT5G02140 37.131 237 127 9 10 225 6 241 1.96e-37 131
MS.gene072805.t1 AT1G70250 34.956 226 118 8 25 225 146 367 7.54e-37 137
MS.gene072805.t1 AT4G24180 36.777 242 110 10 1 209 10 241 7.66e-37 132
MS.gene072805.t1 AT5G40020 30.830 253 135 9 2 225 7 248 1.57e-36 129
MS.gene072805.t1 AT2G28790 35.146 239 127 10 13 224 11 248 5.75e-36 128
MS.gene072805.t1 AT2G28790 35.146 239 127 10 13 224 36 273 6.45e-36 128
MS.gene072805.t1 AT4G24180 37.179 234 103 11 9 209 14 236 8.98e-36 129
MS.gene072805.t1 AT4G36000 45.714 140 68 5 92 225 1 138 2.26e-33 120
MS.gene072805.t1 AT4G36000 45.714 140 68 5 92 225 1 138 2.26e-33 120
MS.gene072805.t1 AT4G18253 30.833 240 137 9 7 225 7 238 1.61e-24 98.2
MS.gene072805.t1 AT4G18250 31.301 246 151 8 1 230 34 277 1.09e-22 96.7
MS.gene072805.t1 AT5G24620 42.623 122 50 5 122 225 15 134 9.68e-20 86.3

Find 86 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCGCCATGGCTCGTATTT+GGG 0.216063 5.3:-4179815 MS.gene072805:CDS
ATGGATTTCTTCCCTCTTAA+TGG 0.218122 5.3:-4179610 MS.gene072805:CDS
GTACCGCCATGGCTCGTATT+TGG 0.222625 5.3:-4179816 MS.gene072805:CDS
AAGGAGTGAGGGTGAGTAGT+AGG 0.288125 5.3:+4179959 None:intergenic
AGAAATCTTGATTTCCGAAT+TGG 0.317167 5.3:+4179668 None:intergenic
ACAATTGCCCTTACACCGTT+TGG 0.330621 5.3:-4179909 MS.gene072805:CDS
AATGAATATTGTTGCACTAA+TGG 0.346009 5.3:-4179484 MS.gene072805:CDS
CCTTGAGTCCTCAATTCCTT+AGG 0.351684 5.3:+4179541 None:intergenic
TGGACTCCAATGTACTGGTT+GGG 0.357055 5.3:-4179728 MS.gene072805:CDS
GCAGCTGCCCAAACGGTGTA+AGG 0.366048 5.3:+4179901 None:intergenic
GAATTGGTTTAACGCATATT+CGG 0.379434 5.3:+4179684 None:intergenic
GGTCGTGGCCGTTGCCAAAC+AGG 0.379579 5.3:-4179763 MS.gene072805:CDS
AACTTTGATGGCAGCGGTCG+TGG 0.386076 5.3:-4179778 MS.gene072805:CDS
TCGAGACGGCGACCACCGCC+TGG 0.399028 5.3:+4179874 None:intergenic
CGCACCGGTTGCAACTTTGA+TGG 0.400828 5.3:-4179790 MS.gene072805:CDS
GCATATTCGGCTAATGTGTT+TGG 0.402845 5.3:+4179697 None:intergenic
ATTAGTGCAACAATATTCAT+TGG 0.423057 5.3:+4179486 None:intergenic
AGTTGCACTGCCGATATTAA+CGG 0.432233 5.3:-4179571 MS.gene072805:CDS
TGGGCTAGCAGCTGCCCAAA+CGG 0.440365 5.3:+4179894 None:intergenic
CAATTGCCCTTACACCGTTT+GGG 0.441211 5.3:-4179908 MS.gene072805:CDS
GCGGTCAAACATGGAACCTC+TGG 0.442503 5.3:-4179849 MS.gene072805:CDS
TTATGACATCCACCATTAAG+AGG 0.444579 5.3:+4179598 None:intergenic
AGTACTTTCACTTGTCCTGC+TGG 0.444797 5.3:-4179373 MS.gene072805:CDS
CACCTGTGCAATCACCTGTT+TGG 0.457468 5.3:+4179749 None:intergenic
AGGAGTGAGGGTGAGTAGTA+GGG 0.465064 5.3:+4179960 None:intergenic
AGGGCACTGTCCGTTAATAT+CGG 0.472010 5.3:+4179561 None:intergenic
CTTGAGTCCTCAATTCCTTA+GGG 0.473616 5.3:+4179542 None:intergenic
GAATATGCGTTAAACCAATT+CGG 0.474473 5.3:-4179682 MS.gene072805:CDS
TATTCGGCTAATGTGTTTGG+TGG 0.475364 5.3:+4179700 None:intergenic
CATTTCAATACACACTGGTC+TGG 0.480509 5.3:+4179290 None:intergenic
AAGTGGGTCCACAGCTTCCT+TGG 0.484930 5.3:+4179461 None:intergenic
TACTTGTTGGATCATCTTGA+GGG 0.496803 5.3:+4179392 None:intergenic
ATTGTTGACGATCTCAAAGT+TGG 0.500722 5.3:+4179927 None:intergenic
GCTGCCATCAAAGTTGCAAC+CGG 0.505235 5.3:+4179786 None:intergenic
GGACTCCAATGTACTGGTTG+GGG 0.507096 5.3:-4179727 MS.gene072805:CDS
ATACGAGCCATGGCGGTACC+AGG 0.507450 5.3:+4179820 None:intergenic
GCAGCTGCTAGCCCAGGCGG+TGG 0.514788 5.3:-4179886 MS.gene072805:CDS
GTTTGGGCAGCTGCTAGCCC+AGG 0.516906 5.3:-4179892 MS.gene072805:CDS
GTCTCGACCGCGGTCAAACA+TGG 0.523542 5.3:-4179858 MS.gene072805:CDS
CGATGGTTTCAACATCCCAA+TGG 0.524206 5.3:-4179629 MS.gene072805:CDS
TTCAGTGGCATCTCAATATG+AGG 0.527367 5.3:+4179313 None:intergenic
TACGACATCTCACTTGTCGA+TGG 0.528359 5.3:-4179646 MS.gene072805:CDS
GGACAAGTGAAAGTACTTGT+TGG 0.531593 5.3:+4179379 None:intergenic
TGCAATCACCTGTTTGGCAA+CGG 0.534123 5.3:+4179755 None:intergenic
GATTTCTTCCCTCTTAATGG+TGG 0.535456 5.3:-4179607 MS.gene072805:CDS
GTACTTGTTGGATCATCTTG+AGG 0.536813 5.3:+4179391 None:intergenic
TACACACTGGTCTGGTTCAG+TGG 0.538018 5.3:+4179298 None:intergenic
GCTCGTATTTGGGGTCGCAC+CGG 0.540712 5.3:-4179805 MS.gene072805:CDS
ATGTTTGACCGCGGTCGAGA+CGG 0.542129 5.3:+4179860 None:intergenic
TGGAACCTCTGGGTTAATCC+TGG 0.543119 5.3:-4179838 MS.gene072805:CDS
GTGGACCCACTTATTTCTCA+AGG 0.546200 5.3:-4179450 MS.gene072805:CDS
TCAAAGTTGGCTGCTTGTGA+AGG 0.547034 5.3:+4179940 None:intergenic
TTAAGAGGGAAGAAATCCAT+TGG 0.547257 5.3:+4179613 None:intergenic
TGGTTGTAACAACCCTTGTA+CGG 0.554084 5.3:-4179518 MS.gene072805:CDS
GGTTGCAACTTTGATGGCAG+CGG 0.554541 5.3:-4179784 MS.gene072805:CDS
TAAGAGGGAAGAAATCCATT+GGG 0.555277 5.3:+4179614 None:intergenic
ACTTTATAGTTAGATCCAGC+AGG 0.563128 5.3:+4179358 None:intergenic
GACAGTGCCCTAAGGAATTG+AGG 0.563268 5.3:-4179549 MS.gene072805:CDS
GTGGACTCCAATGTACTGGT+TGG 0.570296 5.3:-4179729 MS.gene072805:CDS
GGGAGCATAGTGTGAATGTG+AGG 0.572658 5.3:+4179980 None:intergenic
GGTTAATCCTGGTACCGCCA+TGG 0.575016 5.3:-4179827 MS.gene072805:CDS
TATTGTTGCACTAATGGCCA+AGG 0.575870 5.3:-4179478 MS.gene072805:CDS
CAGCTGCCCAAACGGTGTAA+GGG 0.577110 5.3:+4179902 None:intergenic
GGACACCCCAACCAGTACAT+TGG 0.577383 5.3:+4179722 None:intergenic
ACCGCCATGGCTCGTATTTG+GGG 0.579247 5.3:-4179814 MS.gene072805:CDS
CCTAAGGAATTGAGGACTCA+AGG 0.579944 5.3:-4179541 MS.gene072805:CDS
ATTCGGCTAATGTGTTTGGT+GGG 0.589352 5.3:+4179701 None:intergenic
TGCCAAACAGGTGATTGCAC+AGG 0.593503 5.3:-4179751 MS.gene072805:CDS
TGGGCAGCTGCTAGCCCAGG+CGG 0.593879 5.3:-4179889 MS.gene072805:CDS
GCGACCCCAAATACGAGCCA+TGG 0.594263 5.3:+4179810 None:intergenic
ACAGGTGGACTCCAATGTAC+TGG 0.600447 5.3:-4179733 MS.gene072805:CDS
CGGTCAAACATGGAACCTCT+GGG 0.601212 5.3:-4179848 MS.gene072805:CDS
TATGACATCCACCATTAAGA+GGG 0.601387 5.3:+4179599 None:intergenic
CGAGACGGCGACCACCGCCT+GGG 0.601550 5.3:+4179875 None:intergenic
TGGCTGCTTGTGAAGGAGTG+AGG 0.609598 5.3:+4179947 None:intergenic
GGCTGCTTGTGAAGGAGTGA+GGG 0.609799 5.3:+4179948 None:intergenic
CAAACAGGTGATTGCACAGG+TGG 0.617640 5.3:-4179748 MS.gene072805:CDS
TATTAACGGACAGTGCCCTA+AGG 0.626097 5.3:-4179557 MS.gene072805:CDS
GGTGGTCGCCGTCTCGACCG+CGG 0.647393 5.3:-4179868 MS.gene072805:CDS
ACTAATGGCCAAGGAAGCTG+TGG 0.677244 5.3:-4179469 MS.gene072805:CDS
ATACACATTTCAATACACAC+TGG 0.687621 5.3:+4179285 None:intergenic
AGAGGTTCCATGTTTGACCG+CGG 0.689871 5.3:+4179851 None:intergenic
ACCCCAAATACGAGCCATGG+CGG 0.699584 5.3:+4179813 None:intergenic
TCAATATGAGGAGCTCCAAG+AGG 0.715579 5.3:+4179325 None:intergenic
CGGTACCAGGATTAACCCAG+AGG 0.716660 5.3:+4179833 None:intergenic
AAGGAATTGAGGACTCAAGG+TGG 0.730754 5.3:-4179538 MS.gene072805:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTCTCAAGGTTTTTTA+AGG - chr5.3:4179851-4179870 MS.gene072805:CDS 20.0%
! AAAAAACCTTGAGAAATAAG+TGG + chr5.3:4179850-4179869 None:intergenic 25.0%
! AAAAACCTTGAGAAATAAGT+GGG + chr5.3:4179849-4179868 None:intergenic 25.0%
! AATGAATATTGTTGCACTAA+TGG - chr5.3:4179807-4179826 MS.gene072805:CDS 25.0%
! ATTAGTGCAACAATATTCAT+TGG + chr5.3:4179808-4179827 None:intergenic 25.0%
AGAAATCTTGATTTCCGAAT+TGG + chr5.3:4179626-4179645 None:intergenic 30.0%
GAATATGCGTTAAACCAATT+CGG - chr5.3:4179609-4179628 MS.gene072805:CDS 30.0%
! GAATTGGTTTAACGCATATT+CGG + chr5.3:4179610-4179629 None:intergenic 30.0%
!!! TCTCAAGGTTTTTTAAGGAT+AGG - chr5.3:4179856-4179875 MS.gene072805:CDS 30.0%
ACTTTATAGTTAGATCCAGC+AGG + chr5.3:4179936-4179955 None:intergenic 35.0%
ATGGATTTCTTCCCTCTTAA+TGG - chr5.3:4179681-4179700 MS.gene072805:CDS 35.0%
ATTGTTGACGATCTCAAAGT+TGG + chr5.3:4179367-4179386 None:intergenic 35.0%
TAAGAGGGAAGAAATCCATT+GGG + chr5.3:4179680-4179699 None:intergenic 35.0%
TATGACATCCACCATTAAGA+GGG + chr5.3:4179695-4179714 None:intergenic 35.0%
TTAAGAGGGAAGAAATCCAT+TGG + chr5.3:4179681-4179700 None:intergenic 35.0%
TTATGACATCCACCATTAAG+AGG + chr5.3:4179696-4179715 None:intergenic 35.0%
! TACTTGTTGGATCATCTTGA+GGG + chr5.3:4179902-4179921 None:intergenic 35.0%
! TGGTCTTAAAAACCGTACAA+GGG + chr5.3:4179788-4179807 None:intergenic 35.0%
!! AAAGTTGTTTTCTGTCCTCT+TGG - chr5.3:4179951-4179970 MS.gene072805:CDS 35.0%
!! TTGGTCTTAAAAACCGTACA+AGG + chr5.3:4179789-4179808 None:intergenic 35.0%
AGTTGCACTGCCGATATTAA+CGG - chr5.3:4179720-4179739 MS.gene072805:CDS 40.0%
ATTCGGCTAATGTGTTTGGT+GGG + chr5.3:4179593-4179612 None:intergenic 40.0%
CTTGAGTCCTCAATTCCTTA+GGG + chr5.3:4179752-4179771 None:intergenic 40.0%
GATTTCTTCCCTCTTAATGG+TGG - chr5.3:4179684-4179703 MS.gene072805:CDS 40.0%
GCATATTCGGCTAATGTGTT+TGG + chr5.3:4179597-4179616 None:intergenic 40.0%
TATTCGGCTAATGTGTTTGG+TGG + chr5.3:4179594-4179613 None:intergenic 40.0%
TATTGTTGCACTAATGGCCA+AGG - chr5.3:4179813-4179832 MS.gene072805:CDS 40.0%
TGGTTGTAACAACCCTTGTA+CGG - chr5.3:4179773-4179792 MS.gene072805:CDS 40.0%
TTCAGTGGCATCTCAATATG+AGG + chr5.3:4179981-4180000 None:intergenic 40.0%
! GTACTTGTTGGATCATCTTG+AGG + chr5.3:4179903-4179922 None:intergenic 40.0%
!! GGACAAGTGAAAGTACTTGT+TGG + chr5.3:4179915-4179934 None:intergenic 40.0%
AAGGAATTGAGGACTCAAGG+TGG - chr5.3:4179753-4179772 MS.gene072805:CDS 45.0%
ACAATTGCCCTTACACCGTT+TGG - chr5.3:4179382-4179401 MS.gene072805:CDS 45.0%
AGTACTTTCACTTGTCCTGC+TGG - chr5.3:4179918-4179937 MS.gene072805:CDS 45.0%
CAATTGCCCTTACACCGTTT+GGG - chr5.3:4179383-4179402 MS.gene072805:CDS 45.0%
CCTAAGGAATTGAGGACTCA+AGG - chr5.3:4179750-4179769 MS.gene072805:CDS 45.0%
CCTTGAGTCCTCAATTCCTT+AGG + chr5.3:4179753-4179772 None:intergenic 45.0%
CGATGGTTTCAACATCCCAA+TGG - chr5.3:4179662-4179681 MS.gene072805:CDS 45.0%
TACGACATCTCACTTGTCGA+TGG - chr5.3:4179645-4179664 MS.gene072805:CDS 45.0%
TCAAAGTTGGCTGCTTGTGA+AGG + chr5.3:4179354-4179373 None:intergenic 45.0%
TCAATATGAGGAGCTCCAAG+AGG + chr5.3:4179969-4179988 None:intergenic 45.0%
TGCAATCACCTGTTTGGCAA+CGG + chr5.3:4179539-4179558 None:intergenic 45.0%
! GTGGACCCACTTATTTCTCA+AGG - chr5.3:4179841-4179860 MS.gene072805:CDS 45.0%
! TATTAACGGACAGTGCCCTA+AGG - chr5.3:4179734-4179753 MS.gene072805:CDS 45.0%
!! AGGGCACTGTCCGTTAATAT+CGG + chr5.3:4179733-4179752 None:intergenic 45.0%
!! TGGACTCCAATGTACTGGTT+GGG - chr5.3:4179563-4179582 MS.gene072805:CDS 45.0%
AAGGAGTGAGGGTGAGTAGT+AGG + chr5.3:4179335-4179354 None:intergenic 50.0%
ACTAATGGCCAAGGAAGCTG+TGG - chr5.3:4179822-4179841 MS.gene072805:CDS 50.0%
AGAGGTTCCATGTTTGACCG+CGG + chr5.3:4179443-4179462 None:intergenic 50.0%
AGGAGTGAGGGTGAGTAGTA+GGG + chr5.3:4179334-4179353 None:intergenic 50.0%
CAAACAGGTGATTGCACAGG+TGG - chr5.3:4179543-4179562 MS.gene072805:CDS 50.0%
CACCTGTGCAATCACCTGTT+TGG + chr5.3:4179545-4179564 None:intergenic 50.0%
CGGTCAAACATGGAACCTCT+GGG - chr5.3:4179443-4179462 MS.gene072805:CDS 50.0%
GACAGTGCCCTAAGGAATTG+AGG - chr5.3:4179742-4179761 MS.gene072805:CDS 50.0%
GCTGCCATCAAAGTTGCAAC+CGG + chr5.3:4179508-4179527 None:intergenic 50.0%
TACCGCCATGGCTCGTATTT+GGG - chr5.3:4179476-4179495 MS.gene072805:CDS 50.0%
TGCCAAACAGGTGATTGCAC+AGG - chr5.3:4179540-4179559 MS.gene072805:CDS 50.0%
TGGAACCTCTGGGTTAATCC+TGG - chr5.3:4179453-4179472 MS.gene072805:CDS 50.0%
! ACAGGTGGACTCCAATGTAC+TGG - chr5.3:4179558-4179577 MS.gene072805:CDS 50.0%
! GGGAGCATAGTGTGAATGTG+AGG + chr5.3:4179314-4179333 None:intergenic 50.0%
! GGTTGCAACTTTGATGGCAG+CGG - chr5.3:4179507-4179526 MS.gene072805:CDS 50.0%
! GTGGACTCCAATGTACTGGT+TGG - chr5.3:4179562-4179581 MS.gene072805:CDS 50.0%
! TACACACTGGTCTGGTTCAG+TGG + chr5.3:4179996-4180015 None:intergenic 50.0%
!! GGACTCCAATGTACTGGTTG+GGG - chr5.3:4179564-4179583 MS.gene072805:CDS 50.0%
AAGTGGGTCCACAGCTTCCT+TGG + chr5.3:4179833-4179852 None:intergenic 55.0%
ACCCCAAATACGAGCCATGG+CGG + chr5.3:4179481-4179500 None:intergenic 55.0%
ACCGCCATGGCTCGTATTTG+GGG - chr5.3:4179477-4179496 MS.gene072805:CDS 55.0%
ATGTTTGACCGCGGTCGAGA+CGG + chr5.3:4179434-4179453 None:intergenic 55.0%
CAGCTGCCCAAACGGTGTAA+GGG + chr5.3:4179392-4179411 None:intergenic 55.0%
CGGTACCAGGATTAACCCAG+AGG + chr5.3:4179461-4179480 None:intergenic 55.0%
GCGGTCAAACATGGAACCTC+TGG - chr5.3:4179442-4179461 MS.gene072805:CDS 55.0%
GGACACCCCAACCAGTACAT+TGG + chr5.3:4179572-4179591 None:intergenic 55.0%
GGCTGCTTGTGAAGGAGTGA+GGG + chr5.3:4179346-4179365 None:intergenic 55.0%
GGTTAATCCTGGTACCGCCA+TGG - chr5.3:4179464-4179483 MS.gene072805:CDS 55.0%
GTACCGCCATGGCTCGTATT+TGG - chr5.3:4179475-4179494 MS.gene072805:CDS 55.0%
TGGCTGCTTGTGAAGGAGTG+AGG + chr5.3:4179347-4179366 None:intergenic 55.0%
! AACTTTGATGGCAGCGGTCG+TGG - chr5.3:4179513-4179532 MS.gene072805:CDS 55.0%
!! CGCACCGGTTGCAACTTTGA+TGG - chr5.3:4179501-4179520 MS.gene072805:CDS 55.0%
ATACGAGCCATGGCGGTACC+AGG + chr5.3:4179474-4179493 None:intergenic 60.0%
GCAGCTGCCCAAACGGTGTA+AGG + chr5.3:4179393-4179412 None:intergenic 60.0%
GCGACCCCAAATACGAGCCA+TGG + chr5.3:4179484-4179503 None:intergenic 60.0%
GTCTCGACCGCGGTCAAACA+TGG - chr5.3:4179433-4179452 MS.gene072805:CDS 60.0%
! GCTCGTATTTGGGGTCGCAC+CGG - chr5.3:4179486-4179505 MS.gene072805:CDS 60.0%
!! TGGGCTAGCAGCTGCCCAAA+CGG + chr5.3:4179400-4179419 None:intergenic 60.0%
GGTCGTGGCCGTTGCCAAAC+AGG - chr5.3:4179528-4179547 MS.gene072805:CDS 65.0%
GTTTGGGCAGCTGCTAGCCC+AGG - chr5.3:4179399-4179418 MS.gene072805:CDS 65.0%
TGGGCAGCTGCTAGCCCAGG+CGG - chr5.3:4179402-4179421 MS.gene072805:CDS 70.0%
CGAGACGGCGACCACCGCCT+GGG + chr5.3:4179419-4179438 None:intergenic 75.0%
GCAGCTGCTAGCCCAGGCGG+TGG - chr5.3:4179405-4179424 MS.gene072805:CDS 75.0%
GGTGGTCGCCGTCTCGACCG+CGG - chr5.3:4179423-4179442 MS.gene072805:CDS 75.0%
TCGAGACGGCGACCACCGCC+TGG + chr5.3:4179420-4179439 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 4179294 4180019 4179294 ID=MS.gene072805
chr5.3 mRNA 4179294 4180019 4179294 ID=MS.gene072805.t1;Parent=MS.gene072805
chr5.3 exon 4179294 4180019 4179294 ID=MS.gene072805.t1.exon1;Parent=MS.gene072805.t1
chr5.3 CDS 4179294 4180019 4179294 ID=cds.MS.gene072805.t1;Parent=MS.gene072805.t1
Gene Sequence

>MS.gene072805

ATGACTTTCATATCAATCCTCACATTCACACTATGCTCCCTACTACTCACCCTCACTCCTTCACAAGCAGCCAACTTTGAGATCGTCAACAATTGCCCTTACACCGTTTGGGCAGCTGCTAGCCCAGGCGGTGGTCGCCGTCTCGACCGCGGTCAAACATGGAACCTCTGGGTTAATCCTGGTACCGCCATGGCTCGTATTTGGGGTCGCACCGGTTGCAACTTTGATGGCAGCGGTCGTGGCCGTTGCCAAACAGGTGATTGCACAGGTGGACTCCAATGTACTGGTTGGGGTGTCCCACCAAACACATTAGCCGAATATGCGTTAAACCAATTCGGAAATCAAGATTTCTACGACATCTCACTTGTCGATGGTTTCAACATCCCAATGGATTTCTTCCCTCTTAATGGTGGATGTCATAAAATCAGTTGCACTGCCGATATTAACGGACAGTGCCCTAAGGAATTGAGGACTCAAGGTGGTTGTAACAACCCTTGTACGGTTTTTAAGACCAATGAATATTGTTGCACTAATGGCCAAGGAAGCTGTGGACCCACTTATTTCTCAAGGTTTTTTAAGGATAGGTGTCATGATTCATACAGTTACCCTCAAGATGATCCAACAAGTACTTTCACTTGTCCTGCTGGATCTAACTATAAAGTTGTTTTCTGTCCTCTTGGAGCTCCTCATATTGAGATGCCACTGAACCAGACCAGTGTGTATTGA
Protein sequence

>MS.gene072805.t1

MTFISILTFTLCSLLLTLTPSQAANFEIVNNCPYTVWAAASPGGGRRLDRGQTWNLWVNPGTAMARIWGRTGCNFDGSGRGRCQTGDCTGGLQCTGWGVPPNTLAEYALNQFGNQDFYDISLVDGFNIPMDFFPLNGGCHKISCTADINGQCPKELRTQGGCNNPCTVFKTNEYCCTNGQGSCGPTYFSRFFKDRCHDSYSYPQDDPTSTFTCPAGSNYKVVFCPLGAPHIEMPLNQTSVY