AlfalfaGEDB | MS.gene41190

M. sativa cultivar XinJiangDaYe / MS.gene41190

KEGG: -
GO: -
PFAM: PF00314.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41190.t1	XP_013456089.1	96.4	252	9	0	1	252	1	252	1.10E-145	525.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41190.t1	Q41350	66.0	256	79	2	1	252	1	252	7.9e-100	364.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41190.t1	I3SX98	96.4	252	9	0	1	252	1	252	8.0e-146	525.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049239	MS.gene41190	0.800751	1.24E-48	-1.69E-46
MS.gene049298	MS.gene41190	0.853005	3.18E-61	-1.69E-46
MS.gene050707	MS.gene41190	0.845681	3.49E-59	-1.69E-46
MS.gene050710	MS.gene41190	0.808046	3.75E-50	-1.69E-46
MS.gene050875	MS.gene41190	0.803902	2.79E-49	-1.69E-46
MS.gene051914	MS.gene41190	0.864224	1.42E-64	-1.69E-46
MS.gene052209	MS.gene41190	0.80761	4.65E-50	-1.69E-46
MS.gene052308	MS.gene41190	0.873375	1.54E-67	-1.69E-46
MS.gene053108	MS.gene41190	0.825496	4.54E-54	-1.69E-46
MS.gene053173	MS.gene41190	0.824529	7.68E-54	-1.69E-46
MS.gene053348	MS.gene41190	0.837251	5.80E-57	-1.69E-46
MS.gene054551	MS.gene41190	0.845041	5.20E-59	-1.69E-46
MS.gene054593	MS.gene41190	0.844916	5.62E-59	-1.69E-46
MS.gene054633	MS.gene41190	0.844728	6.32E-59	-1.69E-46
MS.gene054850	MS.gene41190	0.868572	5.91E-66	-1.69E-46
MS.gene054941	MS.gene41190	0.850782	1.36E-60	-1.69E-46
MS.gene055308	MS.gene41190	0.85451	1.17E-61	-1.69E-46
MS.gene055578	MS.gene41190	0.8402	1.00E-57	-1.69E-46
MS.gene056041	MS.gene41190	0.829934	3.90E-55	-1.69E-46
MS.gene05640	MS.gene41190	0.87382	1.09E-67	-1.69E-46
MS.gene056528	MS.gene41190	0.82442	8.15E-54	-1.69E-46
MS.gene05652	MS.gene41190	0.890216	1.20E-73	-1.69E-46
MS.gene056618	MS.gene41190	0.822483	2.31E-53	-1.69E-46
MS.gene057101	MS.gene41190	0.838841	2.26E-57	-1.69E-46
MS.gene057356	MS.gene41190	0.801758	7.74E-49	-1.69E-46
MS.gene057669	MS.gene41190	0.853685	2.03E-61	-1.69E-46
MS.gene058128	MS.gene41190	0.935213	1.21E-96	-1.69E-46
MS.gene058789	MS.gene41190	0.811394	7.16E-51	-1.69E-46
MS.gene058853	MS.gene41190	0.891522	3.68E-74	-1.69E-46
MS.gene05926	MS.gene41190	0.827882	1.22E-54	-1.69E-46
MS.gene059616	MS.gene41190	0.893154	8.19E-75	-1.69E-46
MS.gene059905	MS.gene41190	0.878588	2.49E-69	-1.69E-46
MS.gene06093	MS.gene41190	0.830355	3.08E-55	-1.69E-46
MS.gene061089	MS.gene41190	0.832872	7.38E-56	-1.69E-46
MS.gene061099	MS.gene41190	0.805348	1.39E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene41190	MS.gene26931	PPI
MS.gene056186	MS.gene41190	PPI
MS.gene41190	MS.gene59476	PPI
MS.gene016652	MS.gene41190	PPI
MS.gene040126	MS.gene41190	PPI
MS.gene41190	MS.gene69123	PPI
MS.gene41190	MS.gene27345	PPI
MS.gene64778	MS.gene41190	PPI
MS.gene24315	MS.gene41190	PPI
MS.gene45764	MS.gene41190	PPI
MS.gene41190	MS.gene92395	PPI
MS.gene42360	MS.gene41190	PPI
MS.gene41190	MS.gene92397	PPI
MS.gene41190	MS.gene010631	PPI
MS.gene93762	MS.gene41190	PPI
MS.gene20542	MS.gene41190	PPI
MS.gene41190	MS.gene046990	PPI
MS.gene94553	MS.gene41190	PPI
MS.gene41190	MS.gene92393	PPI
MS.gene56731	MS.gene41190	PPI
MS.gene42714	MS.gene41190	PPI
MS.gene41190	MS.gene062716	PPI
MS.gene41190	MS.gene056184	PPI
MS.gene41190	MS.gene68072	PPI
MS.gene41190	MS.gene014545	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41190.t1	MTR_4g063630	96.429	252	9	0	1	252	1	252	3.48e-172	474
MS.gene41190.t1	MTR_1g062590	44.939	247	127	6	7	251	2	241	5.28e-66	205
MS.gene41190.t1	MTR_1g062630	46.154	247	124	6	7	251	2	241	6.33e-66	206
MS.gene41190.t1	MTR_4g073720	45.161	248	121	9	8	251	3	239	3.13e-63	201
MS.gene41190.t1	MTR_1g062340	43.882	237	126	5	17	251	10	241	3.23e-63	198
MS.gene41190.t1	MTR_8g107140	43.775	249	132	6	5	251	2	244	1.01e-62	197
MS.gene41190.t1	MTR_1g062350	42.683	246	134	5	8	251	1	241	2.99e-62	196
MS.gene41190.t1	MTR_1g025420	43.902	246	128	8	12	251	4	245	3.84e-62	196
MS.gene41190.t1	MTR_6g079580	44.215	242	127	7	12	251	4	239	4.56e-62	195
MS.gene41190.t1	MTR_1g025420	43.902	246	128	8	12	251	4	245	4.81e-62	197
MS.gene41190.t1	MTR_1g062660	42.276	246	135	5	8	251	1	241	5.68e-62	195
MS.gene41190.t1	MTR_1g062370	42.276	246	135	5	8	251	1	241	7.13e-62	194
MS.gene41190.t1	MTR_8g036215	41.423	239	129	6	16	251	7	237	3.20e-61	192
MS.gene41190.t1	MTR_7g102380	44.395	223	117	5	31	251	23	240	4.47e-61	192
MS.gene41190.t1	MTR_6g009480	43.852	244	128	7	12	251	13	251	2.60e-59	189
MS.gene41190.t1	MTR_8g075550	45.175	228	114	7	31	251	30	253	3.52e-59	190
MS.gene41190.t1	MTR_8g037890	42.248	258	130	10	1	251	1	246	4.69e-59	190
MS.gene41190.t1	MTR_3g114030	44.889	225	116	7	31	251	9	229	5.03e-59	187
MS.gene41190.t1	MTR_5g059200	42.688	253	122	10	7	251	2	239	4.28e-58	186
MS.gene41190.t1	MTR_4g073730	40.741	243	138	6	12	251	8	247	6.81e-58	186
MS.gene41190.t1	MTR_4g073730	40.741	243	138	6	12	251	8	247	1.44e-57	186
MS.gene41190.t1	MTR_3g068015	38.889	252	141	5	8	251	1	247	6.61e-57	182
MS.gene41190.t1	MTR_5g022350	45.740	223	106	8	31	251	33	242	6.88e-56	181
MS.gene41190.t1	MTR_8g056820	40.625	256	134	10	3	251	2	246	1.20e-55	179
MS.gene41190.t1	MTR_5g022310	41.313	259	135	11	1	251	1	250	3.70e-55	178
MS.gene41190.t1	MTR_1g021945	44.828	232	112	9	26	251	23	244	5.30e-55	177
MS.gene41190.t1	MTR_5g022310	41.313	259	135	11	1	251	1	250	8.71e-55	178
MS.gene41190.t1	MTR_2g069660	40.741	243	136	6	12	251	5	242	1.58e-54	176
MS.gene41190.t1	MTR_7g062610	37.805	246	146	5	7	251	2	241	2.41e-54	175
MS.gene41190.t1	MTR_8g075510	41.736	242	123	8	12	251	18	243	2.74e-54	175
MS.gene41190.t1	MTR_8g075510	41.736	242	123	8	12	251	18	243	7.86e-54	176
MS.gene41190.t1	MTR_3g111620	41.048	229	125	7	26	251	24	245	1.56e-52	171
MS.gene41190.t1	MTR_1g062690	37.143	245	125	4	8	251	1	217	1.31e-49	162
MS.gene41190.t1	MTR_2g068030	41.410	227	121	8	31	252	25	244	1.00e-48	161
MS.gene41190.t1	MTR_2g067980	40.726	248	128	9	16	251	6	246	3.70e-47	167
MS.gene41190.t1	MTR_2g068655	39.827	231	127	8	26	251	28	251	4.98e-47	159
MS.gene41190.t1	MTR_5g010635	37.991	229	116	10	25	251	20	224	1.13e-38	135
MS.gene41190.t1	MTR_8g096900	34.016	244	131	9	13	251	29	247	2.56e-37	132
MS.gene41190.t1	MTR_5g010640	35.223	247	131	10	14	251	9	235	6.26e-36	127
MS.gene41190.t1	MTR_2g063150	34.310	239	140	8	6	233	2	234	6.96e-36	128
MS.gene41190.t1	MTR_8g089020	45.038	131	65	3	111	241	31	154	2.36e-33	119
MS.gene41190.t1	MTR_5g023850	37.755	196	106	9	1	189	1	187	1.32e-31	116
MS.gene41190.t1	MTR_1g062390	32.819	259	137	11	8	251	1	237	2.07e-31	116
MS.gene41190.t1	MTR_2g067990	38.326	227	100	13	31	251	29	221	2.58e-30	119
MS.gene41190.t1	MTR_5g022350	48.855	131	59	5	31	159	98	222	1.71e-29	110
MS.gene41190.t1	MTR_2g067970	37.297	185	102	8	74	251	4	181	1.20e-28	114
MS.gene41190.t1	MTR_7g076360	29.956	227	90	6	22	246	1	160	6.77e-25	97.4
MS.gene41190.t1	MTR_8g096910	40.288	139	71	5	7	144	2	129	1.03e-22	91.7
MS.gene41190.t1	MTR_8g096920	35.758	165	91	7	11	174	2	152	5.04e-20	84.7
MS.gene41190.t1	MTR_1g062640	42.742	124	61	4	49	170	32	147	2.74e-18	79.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41190.t1	AT2G28790	66.393	244	76	3	13	252	33	274	4.32e-114	328
MS.gene41190.t1	AT2G28790	66.393	244	76	3	13	252	8	249	4.78e-114	327
MS.gene41190.t1	AT4G38660	43.083	253	132	6	1	251	2	244	1.51e-62	199
MS.gene41190.t1	AT5G40020	41.732	254	138	6	1	251	1	247	1.10e-61	194
MS.gene41190.t1	AT1G73620	45.815	227	116	5	26	251	39	259	3.32e-61	194
MS.gene41190.t1	AT4G38660	44.397	232	117	6	22	251	1	222	9.09e-61	194
MS.gene41190.t1	AT1G77700	43.750	240	117	9	17	251	45	271	1.68e-59	191
MS.gene41190.t1	AT1G18250	44.934	227	118	5	26	251	19	239	2.15e-59	188
MS.gene41190.t1	AT1G20030	45.575	226	115	7	31	251	5	227	2.31e-59	190
MS.gene41190.t1	AT1G77700	43.750	240	117	9	17	251	45	271	2.65e-59	191
MS.gene41190.t1	AT1G20030	45.575	226	115	7	31	251	22	244	3.42e-59	190
MS.gene41190.t1	AT1G18250	44.541	229	120	5	24	251	16	238	4.27e-59	187
MS.gene41190.t1	AT1G75800	44.490	245	125	8	12	251	9	247	6.29e-59	190
MS.gene41190.t1	AT1G75040	43.902	246	122	10	10	251	5	238	1.26e-58	186
MS.gene41190.t1	AT5G24620	42.979	235	127	5	22	251	18	250	1.51e-58	191
MS.gene41190.t1	AT2G17860	41.057	246	134	7	14	251	6	248	3.15e-58	186
MS.gene41190.t1	AT5G24620	42.979	235	127	5	22	251	94	326	5.87e-58	192
MS.gene41190.t1	AT5G24620	42.979	235	127	5	22	251	73	305	6.04e-58	191
MS.gene41190.t1	AT1G19320	41.365	249	132	7	9	251	6	246	6.49e-58	184
MS.gene41190.t1	AT1G77700	44.000	225	112	8	28	251	88	299	7.54e-57	185
MS.gene41190.t1	AT1G75030	42.387	243	134	6	10	251	5	242	8.86e-57	182
MS.gene41190.t1	AT4G38670	38.554	249	144	7	8	251	2	246	3.78e-56	181
MS.gene41190.t1	AT4G38670	38.554	249	144	7	8	251	2	246	3.78e-56	181
MS.gene41190.t1	AT1G75050	43.673	245	128	9	10	251	5	242	1.56e-55	178
MS.gene41190.t1	AT4G36010	42.424	231	117	8	31	251	25	249	5.59e-55	179
MS.gene41190.t1	AT4G36010	42.424	231	117	8	31	251	25	249	5.59e-55	179
MS.gene41190.t1	AT4G24180	41.600	250	123	9	12	251	14	250	6.13e-55	177
MS.gene41190.t1	AT4G24180	41.600	250	123	9	12	251	14	250	1.37e-54	178
MS.gene41190.t1	AT4G24180	42.105	247	121	8	12	248	14	248	4.35e-54	177
MS.gene41190.t1	AT4G38670	40.000	230	129	7	27	251	5	230	8.00e-54	175
MS.gene41190.t1	AT4G24180	40.873	252	127	9	12	251	14	255	8.07e-54	174
MS.gene41190.t1	AT4G24180	40.873	252	127	9	12	251	14	255	1.69e-53	175
MS.gene41190.t1	AT4G24180	41.365	249	125	8	12	248	14	253	6.00e-53	174
MS.gene41190.t1	AT5G02140	39.662	237	136	5	17	251	9	240	9.33e-52	169
MS.gene41190.t1	AT5G02140	39.662	237	136	5	17	251	9	240	1.54e-51	170
MS.gene41190.t1	AT5G38280	37.143	245	142	8	12	251	12	249	8.42e-43	154
MS.gene41190.t1	AT5G38280	37.143	245	142	8	12	251	12	249	9.50e-43	154
MS.gene41190.t1	AT4G11650	34.694	245	131	12	11	251	3	222	4.80e-37	131
MS.gene41190.t1	AT4G18253	35.124	242	141	6	12	251	10	237	5.05e-36	128
MS.gene41190.t1	AT4G36000	41.611	149	74	5	104	251	1	137	2.34e-34	123
MS.gene41190.t1	AT4G36000	41.611	149	74	5	104	251	1	137	2.34e-34	123
MS.gene41190.t1	AT4G18250	37.037	243	137	9	12	251	42	271	6.33e-34	129
MS.gene41190.t1	AT1G70250	33.333	228	137	9	31	251	147	366	1.27e-28	114
MS.gene41190.t1	AT5G24620	44.167	120	62	3	136	251	15	133	3.23e-24	99.0
MS.gene41190.t1	AT2G24810	46.575	73	38	1	179	251	121	192	5.41e-15	71.6

Find 99 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTTACAAAGGTGAATATT+AGG	0.192517	4.1:-36929234	None:intergenic
AAGTTTGTGGGTTGTTAAAA+TGG	0.224217	4.1:-36929756	None:intergenic
CGGCCGGCCCAGTGGGTGTC+TGG	0.228338	4.1:-36929364	None:intergenic
TGTGTACTCCAAGTTCTTTA+AGG	0.270049	4.1:+36929789	MS.gene41190:CDS
GGCTTGAGAGGGTGAGAATA+TGG	0.325859	4.1:-36929212	None:intergenic
GGTGGTGGAGCAGCCGGTTC+TGG	0.335053	4.1:-36929395	None:intergenic
TTCTTCACCGGGTGAACTTA+AGG	0.359396	4.1:+36929870	MS.gene41190:CDS
GTTTACAAAGGTGAATATTA+GGG	0.376062	4.1:-36929233	None:intergenic
TTCCCGTCCCAGACACCCAC+TGG	0.378709	4.1:+36929356	MS.gene41190:CDS
CCCACTGGGCCGGCCGTGCA+TGG	0.379602	4.1:+36929371	MS.gene41190:CDS
AATGAGAAGTGAGTTGGTTA+AGG	0.388408	4.1:-36929170	None:intergenic
GGCCGTGCATGGGCCAGAAC+CGG	0.396060	4.1:+36929382	MS.gene41190:CDS
GGAACACGGTGCTGCAAAAC+AGG	0.402377	4.1:-36929658	None:intergenic
GATGTACGTCGAACTGTACT+AGG	0.403471	4.1:-36929498	None:intergenic
GCCCAGTGGGTGTCTGGGAC+GGG	0.404695	4.1:-36929358	None:intergenic
TCGAACTGTACTAGGGTTGC+AGG	0.406854	4.1:-36929490	None:intergenic
GGAGAATTTGTTGTTGGTGG+TGG	0.409407	4.1:-36929410	None:intergenic
GGCCCAGTGGGTGTCTGGGA+CGG	0.412521	4.1:-36929359	None:intergenic
ACAGTGATGAACATTGTTGT+AGG	0.414434	4.1:+36929731	MS.gene41190:CDS
GGCCGGCCCAGTGGGTGTCT+GGG	0.418213	4.1:-36929363	None:intergenic
TTCATCTTACAGTGTTAGTC+TGG	0.423960	4.1:+36929537	MS.gene41190:CDS
CCGCCGTTGGGGATGATAGC+TGG	0.427338	4.1:-36929277	None:intergenic
GCCACAGTCGCCGGTGAGGC+AGG	0.428001	4.1:-36929431	None:intergenic
GATTCATCAGTGTTCTTCAC+CGG	0.433172	4.1:+36929858	MS.gene41190:CDS
CCATGCACGGCCGGCCCAGT+GGG	0.440420	4.1:-36929371	None:intergenic
TGTGCAAACGTAAACGTTGC+AGG	0.441491	4.1:-36929820	None:intergenic
CAGTAGGTTTACAAGTTTGT+GGG	0.445854	4.1:-36929768	None:intergenic
GTGGGTGTCTGGGACGGGAA+TGG	0.448638	4.1:-36929353	None:intergenic
GTGAGAATATGGTGAGAAAG+AGG	0.460599	4.1:-36929201	None:intergenic
AGGACAGGGAAGCCGCCGTT+GGG	0.463976	4.1:-36929289	None:intergenic
ACAACTGCCCCTACACCGTC+TGG	0.465738	4.1:+36929254	MS.gene41190:CDS
TCCTGCCTCACCGGCGACTG+TGG	0.467463	4.1:+36929430	MS.gene41190:CDS
GCAGGAGAATTTGTTGTTGG+TGG	0.473524	4.1:-36929413	None:intergenic
GGTTCTGGCCCATGCACGGC+CGG	0.475650	4.1:-36929380	None:intergenic
TTTGCAGCACCGTGTTCCAA+TGG	0.480332	4.1:+36929663	MS.gene41190:CDS
TTGCAGCACCGTGTTCCAAT+GGG	0.481047	4.1:+36929664	MS.gene41190:CDS
ACAAGCATCCTTAAAGAACT+TGG	0.481493	4.1:-36929797	None:intergenic
AACAAATTCTCCTGCCTCAC+CGG	0.484751	4.1:+36929421	MS.gene41190:CDS
ATAGCTGGCCAGACGGTGTA+GGG	0.485266	4.1:-36929262	None:intergenic
CCCATGCACGGCCGGCCCAG+TGG	0.493544	4.1:-36929372	None:intergenic
GTTGTTGGTGGTGGAGCAGC+CGG	0.496475	4.1:-36929401	None:intergenic
CCACTGGGCCGGCCGTGCAT+GGG	0.498018	4.1:+36929372	MS.gene41190:CDS
CTGGGACGGGAATGGAGAGG+TGG	0.501641	4.1:-36929345	None:intergenic
AAGCCGCCGGTAGAGAGGAC+AGG	0.504282	4.1:-36929304	None:intergenic
AGCCGGTTCTGGCCCATGCA+CGG	0.506037	4.1:-36929384	None:intergenic
TCTACCGGCGGCTTCGAACT+CGG	0.506461	4.1:+36929313	MS.gene41190:CDS
CCACTCTTACAAGCCACAAC+AGG	0.507576	4.1:-36929694	None:intergenic
CGTCTCAATTGCAACGGAGC+AGG	0.509039	4.1:+36929457	MS.gene41190:CDS
TGTCTGGGACGGGAATGGAG+AGG	0.512733	4.1:-36929348	None:intergenic
ACAGTAGGTTTACAAGTTTG+TGG	0.515723	4.1:-36929769	None:intergenic
AAAGGTGAGTGTCCCGTTGT+GGG	0.517592	4.1:+36929604	MS.gene41190:CDS
GTCCCAGACACCCACTGGGC+CGG	0.518235	4.1:+36929361	MS.gene41190:CDS
GAGGCAGGAGAATTTGTTGT+TGG	0.520383	4.1:-36929416	None:intergenic
GATAGCTGGCCAGACGGTGT+AGG	0.521167	4.1:-36929263	None:intergenic
TCAGCTTTACAACCCACAAC+GGG	0.523738	4.1:-36929616	None:intergenic
CCTGTTGTGGCTTGTAAGAG+TGG	0.525720	4.1:+36929694	MS.gene41190:CDS
TAAAGGTGAGTGTCCCGTTG+TGG	0.526721	4.1:+36929603	MS.gene41190:CDS
GAATATGGTGAGAAAGAGGA+AGG	0.526737	4.1:-36929197	None:intergenic
GGTAGAAATGAGAAGTGAGT+TGG	0.527432	4.1:-36929176	None:intergenic
GAGGACAGGGAAGCCGCCGT+TGG	0.529494	4.1:-36929290	None:intergenic
GAACACTGATGAATCAAAGA+AGG	0.532789	4.1:-36929850	None:intergenic
GGCTTCCCTGTCCTCTCTAC+CGG	0.537902	4.1:+36929298	MS.gene41190:CDS
TACAGTGTTAGTCTGGTCGA+TGG	0.538986	4.1:+36929544	MS.gene41190:CDS
GAGACGGTTGCCACAGTCGC+CGG	0.545434	4.1:-36929440	None:intergenic
AGCCGCCGGTAGAGAGGACA+GGG	0.552904	4.1:-36929303	None:intergenic
CACCGTGTTCCAATGGGTCA+TGG	0.555886	4.1:+36929670	MS.gene41190:CDS
GGTGAATATTAGGGCTTGAG+AGG	0.569518	4.1:-36929224	None:intergenic
ATTCATCAGTGTTCTTCACC+GGG	0.570673	4.1:+36929859	MS.gene41190:CDS
GTTACACCGCATGAGGGTAA+AGG	0.572327	4.1:+36929586	MS.gene41190:CDS
TTTGACTGTTACACCGCATG+AGG	0.572400	4.1:+36929579	MS.gene41190:CDS
ACAACAGGACCATGACCCAT+TGG	0.572570	4.1:-36929679	None:intergenic
AATGGGTCATGGTCCTGTTG+TGG	0.574417	4.1:+36929681	MS.gene41190:CDS
ATGTACGTCGAACTGTACTA+GGG	0.577377	4.1:-36929497	None:intergenic
CTGCTCCGTTGCAATTGAGA+CGG	0.579237	4.1:-36929456	None:intergenic
TGGGACGGGAATGGAGAGGT+GGG	0.579840	4.1:-36929344	None:intergenic
AAGAACTTGGAGTACACAGT+AGG	0.581786	4.1:-36929784	None:intergenic
TCCCGTCCCAGACACCCACT+GGG	0.584127	4.1:+36929357	MS.gene41190:CDS
CTCAATTGCAACGGAGCAGG+TGG	0.588039	4.1:+36929460	MS.gene41190:CDS
GTTCGAAGCCGCCGGTAGAG+AGG	0.593228	4.1:-36929309	None:intergenic
TCATGCGGTGTAACAGTCAA+AGG	0.597597	4.1:-36929577	None:intergenic
GGCAACCGTCTCAATTGCAA+CGG	0.619867	4.1:+36929451	MS.gene41190:CDS
CACTCACCTTTACCCTCATG+CGG	0.622292	4.1:-36929592	None:intergenic
GTGAATATTAGGGCTTGAGA+GGG	0.628563	4.1:-36929223	None:intergenic
GGGGATGATAGCTGGCCAGA+CGG	0.631024	4.1:-36929269	None:intergenic
GTGACCGAGTTCGAAGCCGC+CGG	0.631127	4.1:-36929317	None:intergenic
GACCATGACCCATTGGAACA+CGG	0.632888	4.1:-36929672	None:intergenic
ATCAGCTTTACAACCCACAA+CGG	0.633015	4.1:-36929617	None:intergenic
GTAGGGGCAGTTGTTTACAA+AGG	0.637176	4.1:-36929245	None:intergenic
GGACAGGGAAGCCGCCGTTG+GGG	0.640158	4.1:-36929288	None:intergenic
AAGATAACCTTAAGTTCACC+CGG	0.642887	4.1:-36929877	None:intergenic
TGGCCAGCTATCATCCCCAA+CGG	0.650509	4.1:+36929274	MS.gene41190:CDS
TTGACTGTTACACCGCATGA+GGG	0.659652	4.1:+36929580	MS.gene41190:CDS
TTCCCTGTCCTCTCTACCGG+CGG	0.660864	4.1:+36929301	MS.gene41190:CDS
GGTTGCCACAGTCGCCGGTG+AGG	0.664371	4.1:-36929435	None:intergenic
TAGCTGGCCAGACGGTGTAG+GGG	0.666219	4.1:-36929261	None:intergenic
CCAGCTATCATCCCCAACGG+CGG	0.674088	4.1:+36929277	MS.gene41190:CDS
AACTGTACTAGGGTTGCAGG+TGG	0.674287	4.1:-36929487	None:intergenic
GTACAGTTCGACGTACATCA+CGG	0.678213	4.1:+36929502	MS.gene41190:CDS
ACACGGTGCTGCAAAACAGG+AGG	0.685097	4.1:-36929655	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTTACAAAGGTGAATATTA+GGG	-	chr4.1:36929236-36929255	None:intergenic	25.0%
!	TGTTTACAAAGGTGAATATT+AGG	-	chr4.1:36929237-36929256	None:intergenic	25.0%
!	AAGTTTGTGGGTTGTTAAAA+TGG	-	chr4.1:36929759-36929778	None:intergenic	30.0%
	AAGATAACCTTAAGTTCACC+CGG	-	chr4.1:36929880-36929899	None:intergenic	35.0%
	ACAAGCATCCTTAAAGAACT+TGG	-	chr4.1:36929800-36929819	None:intergenic	35.0%
	ACAGTAGGTTTACAAGTTTG+TGG	-	chr4.1:36929772-36929791	None:intergenic	35.0%
	ACAGTGATGAACATTGTTGT+AGG	+	chr4.1:36929731-36929750	MS.gene41190:CDS	35.0%
	CAGTAGGTTTACAAGTTTGT+GGG	-	chr4.1:36929771-36929790	None:intergenic	35.0%
	GAACACTGATGAATCAAAGA+AGG	-	chr4.1:36929853-36929872	None:intergenic	35.0%
	TGTGTACTCCAAGTTCTTTA+AGG	+	chr4.1:36929789-36929808	MS.gene41190:CDS	35.0%
	TTCATCTTACAGTGTTAGTC+TGG	+	chr4.1:36929537-36929556	MS.gene41190:CDS	35.0%
!!	AATGAGAAGTGAGTTGGTTA+AGG	-	chr4.1:36929173-36929192	None:intergenic	35.0%
	AAGAACTTGGAGTACACAGT+AGG	-	chr4.1:36929787-36929806	None:intergenic	40.0%
	ATCAGCTTTACAACCCACAA+CGG	-	chr4.1:36929620-36929639	None:intergenic	40.0%
	ATGTACGTCGAACTGTACTA+GGG	-	chr4.1:36929500-36929519	None:intergenic	40.0%
	ATTCATCAGTGTTCTTCACC+GGG	+	chr4.1:36929859-36929878	MS.gene41190:CDS	40.0%
	GAATATGGTGAGAAAGAGGA+AGG	-	chr4.1:36929200-36929219	None:intergenic	40.0%
	GATTCATCAGTGTTCTTCAC+CGG	+	chr4.1:36929858-36929877	MS.gene41190:CDS	40.0%
	GGTAGAAATGAGAAGTGAGT+TGG	-	chr4.1:36929179-36929198	None:intergenic	40.0%
	GTGAATATTAGGGCTTGAGA+GGG	-	chr4.1:36929226-36929245	None:intergenic	40.0%
	GTGAGAATATGGTGAGAAAG+AGG	-	chr4.1:36929204-36929223	None:intergenic	40.0%
	AACAAATTCTCCTGCCTCAC+CGG	+	chr4.1:36929421-36929440	MS.gene41190:CDS	45.0%
	GATGTACGTCGAACTGTACT+AGG	-	chr4.1:36929501-36929520	None:intergenic	45.0%
	GGTGAATATTAGGGCTTGAG+AGG	-	chr4.1:36929227-36929246	None:intergenic	45.0%
	GTACAGTTCGACGTACATCA+CGG	+	chr4.1:36929502-36929521	MS.gene41190:CDS	45.0%
	GTAGGGGCAGTTGTTTACAA+AGG	-	chr4.1:36929248-36929267	None:intergenic	45.0%
	TCAGCTTTACAACCCACAAC+GGG	-	chr4.1:36929619-36929638	None:intergenic	45.0%
	TCATGCGGTGTAACAGTCAA+AGG	-	chr4.1:36929580-36929599	None:intergenic	45.0%
	TGTGCAAACGTAAACGTTGC+AGG	-	chr4.1:36929823-36929842	None:intergenic	45.0%
	TTCTTCACCGGGTGAACTTA+AGG	+	chr4.1:36929870-36929889	MS.gene41190:CDS	45.0%
	TTGACTGTTACACCGCATGA+GGG	+	chr4.1:36929580-36929599	MS.gene41190:CDS	45.0%
	TTTGACTGTTACACCGCATG+AGG	+	chr4.1:36929579-36929598	MS.gene41190:CDS	45.0%
!	TACAGTGTTAGTCTGGTCGA+TGG	+	chr4.1:36929544-36929563	MS.gene41190:CDS	45.0%
!!	GAGGCAGGAGAATTTGTTGT+TGG	-	chr4.1:36929419-36929438	None:intergenic	45.0%
!!	GCAGGAGAATTTGTTGTTGG+TGG	-	chr4.1:36929416-36929435	None:intergenic	45.0%
!!	GGAGAATTTGTTGTTGGTGG+TGG	-	chr4.1:36929413-36929432	None:intergenic	45.0%
	AAAGGTGAGTGTCCCGTTGT+GGG	+	chr4.1:36929604-36929623	MS.gene41190:CDS	50.0%
	AACTGTACTAGGGTTGCAGG+TGG	-	chr4.1:36929490-36929509	None:intergenic	50.0%
	AATGGGTCATGGTCCTGTTG+TGG	+	chr4.1:36929681-36929700	MS.gene41190:CDS	50.0%
	ACAACAGGACCATGACCCAT+TGG	-	chr4.1:36929682-36929701	None:intergenic	50.0%
	CACTCACCTTTACCCTCATG+CGG	-	chr4.1:36929595-36929614	None:intergenic	50.0%
	CCACTCTTACAAGCCACAAC+AGG	-	chr4.1:36929697-36929716	None:intergenic	50.0%
	CTGCTCCGTTGCAATTGAGA+CGG	-	chr4.1:36929459-36929478	None:intergenic	50.0%
	GACCATGACCCATTGGAACA+CGG	-	chr4.1:36929675-36929694	None:intergenic	50.0%
	GGCAACCGTCTCAATTGCAA+CGG	+	chr4.1:36929451-36929470	MS.gene41190:CDS	50.0%
	GGCTTGAGAGGGTGAGAATA+TGG	-	chr4.1:36929215-36929234	None:intergenic	50.0%
	GTTACACCGCATGAGGGTAA+AGG	+	chr4.1:36929586-36929605	MS.gene41190:CDS	50.0%
	TAAAGGTGAGTGTCCCGTTG+TGG	+	chr4.1:36929603-36929622	MS.gene41190:CDS	50.0%
	TCGAACTGTACTAGGGTTGC+AGG	-	chr4.1:36929493-36929512	None:intergenic	50.0%
	TTGCAGCACCGTGTTCCAAT+GGG	+	chr4.1:36929664-36929683	MS.gene41190:CDS	50.0%
	TTTGCAGCACCGTGTTCCAA+TGG	+	chr4.1:36929663-36929682	MS.gene41190:CDS	50.0%
!	CCTGTTGTGGCTTGTAAGAG+TGG	+	chr4.1:36929694-36929713	MS.gene41190:CDS	50.0%
	ATAGCTGGCCAGACGGTGTA+GGG	-	chr4.1:36929265-36929284	None:intergenic	55.0%
	CACCGTGTTCCAATGGGTCA+TGG	+	chr4.1:36929670-36929689	MS.gene41190:CDS	55.0%
	CGTCTCAATTGCAACGGAGC+AGG	+	chr4.1:36929457-36929476	MS.gene41190:CDS	55.0%
	CTCAATTGCAACGGAGCAGG+TGG	+	chr4.1:36929460-36929479	MS.gene41190:CDS	55.0%
	TGGCCAGCTATCATCCCCAA+CGG	+	chr4.1:36929274-36929293	MS.gene41190:CDS	55.0%
!!	ACACGGTGCTGCAAAACAGG+AGG	-	chr4.1:36929658-36929677	None:intergenic	55.0%
!!	GGAACACGGTGCTGCAAAAC+AGG	-	chr4.1:36929661-36929680	None:intergenic	55.0%
	ACAACTGCCCCTACACCGTC+TGG	+	chr4.1:36929254-36929273	MS.gene41190:CDS	60.0%
	CCAGCTATCATCCCCAACGG+CGG	+	chr4.1:36929277-36929296	MS.gene41190:CDS	60.0%
	GATAGCTGGCCAGACGGTGT+AGG	-	chr4.1:36929266-36929285	None:intergenic	60.0%
	GGCTTCCCTGTCCTCTCTAC+CGG	+	chr4.1:36929298-36929317	MS.gene41190:CDS	60.0%
	GGGGATGATAGCTGGCCAGA+CGG	-	chr4.1:36929272-36929291	None:intergenic	60.0%
	TAGCTGGCCAGACGGTGTAG+GGG	-	chr4.1:36929264-36929283	None:intergenic	60.0%
	TCTACCGGCGGCTTCGAACT+CGG	+	chr4.1:36929313-36929332	MS.gene41190:CDS	60.0%
	TGGGACGGGAATGGAGAGGT+GGG	-	chr4.1:36929347-36929366	None:intergenic	60.0%
	TTCCCTGTCCTCTCTACCGG+CGG	+	chr4.1:36929301-36929320	MS.gene41190:CDS	60.0%
!	TGTCTGGGACGGGAATGGAG+AGG	-	chr4.1:36929351-36929370	None:intergenic	60.0%
!!	GTTGTTGGTGGTGGAGCAGC+CGG	-	chr4.1:36929404-36929423	None:intergenic	60.0%
	AAGCCGCCGGTAGAGAGGAC+AGG	-	chr4.1:36929307-36929326	None:intergenic	65.0%
	AGCCGCCGGTAGAGAGGACA+GGG	-	chr4.1:36929306-36929325	None:intergenic	65.0%
	AGGACAGGGAAGCCGCCGTT+GGG	-	chr4.1:36929292-36929311	None:intergenic	65.0%
	CCGCCGTTGGGGATGATAGC+TGG	-	chr4.1:36929280-36929299	None:intergenic	65.0%
	CTGGGACGGGAATGGAGAGG+TGG	-	chr4.1:36929348-36929367	None:intergenic	65.0%
	GAGACGGTTGCCACAGTCGC+CGG	-	chr4.1:36929443-36929462	None:intergenic	65.0%
	GTGACCGAGTTCGAAGCCGC+CGG	-	chr4.1:36929320-36929339	None:intergenic	65.0%
	GTTCGAAGCCGCCGGTAGAG+AGG	-	chr4.1:36929312-36929331	None:intergenic	65.0%
	TCCCGTCCCAGACACCCACT+GGG	+	chr4.1:36929357-36929376	MS.gene41190:CDS	65.0%
	TTCCCGTCCCAGACACCCAC+TGG	+	chr4.1:36929356-36929375	MS.gene41190:CDS	65.0%
!	AGCCGGTTCTGGCCCATGCA+CGG	-	chr4.1:36929387-36929406	None:intergenic	65.0%
!	GTGGGTGTCTGGGACGGGAA+TGG	-	chr4.1:36929356-36929375	None:intergenic	65.0%
	GAGGACAGGGAAGCCGCCGT+TGG	-	chr4.1:36929293-36929312	None:intergenic	70.0%
	GGACAGGGAAGCCGCCGTTG+GGG	-	chr4.1:36929291-36929310	None:intergenic	70.0%
	GGCCCAGTGGGTGTCTGGGA+CGG	-	chr4.1:36929362-36929381	None:intergenic	70.0%
	GGCCGTGCATGGGCCAGAAC+CGG	+	chr4.1:36929382-36929401	MS.gene41190:CDS	70.0%
	GGTTGCCACAGTCGCCGGTG+AGG	-	chr4.1:36929438-36929457	None:intergenic	70.0%
	GTCCCAGACACCCACTGGGC+CGG	+	chr4.1:36929361-36929380	MS.gene41190:CDS	70.0%
	TCCTGCCTCACCGGCGACTG+TGG	+	chr4.1:36929430-36929449	MS.gene41190:CDS	70.0%
!	GCCCAGTGGGTGTCTGGGAC+GGG	-	chr4.1:36929361-36929380	None:intergenic	70.0%
!	GGTTCTGGCCCATGCACGGC+CGG	-	chr4.1:36929383-36929402	None:intergenic	70.0%
!!	GGTGGTGGAGCAGCCGGTTC+TGG	-	chr4.1:36929398-36929417	None:intergenic	70.0%
	CCACTGGGCCGGCCGTGCAT+GGG	+	chr4.1:36929372-36929391	MS.gene41190:CDS	75.0%
	CCATGCACGGCCGGCCCAGT+GGG	-	chr4.1:36929374-36929393	None:intergenic	75.0%
	GCCACAGTCGCCGGTGAGGC+AGG	-	chr4.1:36929434-36929453	None:intergenic	75.0%
	GGCCGGCCCAGTGGGTGTCT+GGG	-	chr4.1:36929366-36929385	None:intergenic	75.0%
!	CCCACTGGGCCGGCCGTGCA+TGG	+	chr4.1:36929371-36929390	MS.gene41190:CDS	80.0%
!	CCCATGCACGGCCGGCCCAG+TGG	-	chr4.1:36929375-36929394	None:intergenic	80.0%
!	CGGCCGGCCCAGTGGGTGTC+TGG	-	chr4.1:36929367-36929386	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	36929151	36929909	36929151	ID=MS.gene41190
chr4.1	mRNA	36929151	36929909	36929151	ID=MS.gene41190.t1;Parent=MS.gene41190
chr4.1	exon	36929151	36929909	36929151	ID=MS.gene41190.t1.exon1;Parent=MS.gene41190.t1
chr4.1	CDS	36929151	36929909	36929151	ID=cds.MS.gene41190.t1;Parent=MS.gene41190.t1

Gene Sequence

>MS.gene41190

ATGGCTTCTTCTTCTTCTTCCTTAACCAACTCACTTCTCATTTCTACCTTCCTCTTTCTCACCATATTCTCACCCTCTCAAGCCCTAATATTCACCTTTGTAAACAACTGCCCCTACACCGTCTGGCCAGCTATCATCCCCAACGGCGGCTTCCCTGTCCTCTCTACCGGCGGCTTCGAACTCGGTCACTTCACCCACCTCTCCATTCCCGTCCCAGACACCCACTGGGCCGGCCGTGCATGGGCCAGAACCGGCTGCTCCACCACCAACAACAAATTCTCCTGCCTCACCGGCGACTGTGGCAACCGTCTCAATTGCAACGGAGCAGGTGGTTCACCACCTGCAACCCTAGTACAGTTCGACGTACATCACGGTAACAAAGACTTTTCATCTTACAGTGTTAGTCTGGTCGATGGTTTCAACACTCCTTTGACTGTTACACCGCATGAGGGTAAAGGTGAGTGTCCCGTTGTGGGTTGTAAAGCTGATCTTGTTGCTTCATGTCCTCCTGTTTTGCAGCACCGTGTTCCAATGGGTCATGGTCCTGTTGTGGCTTGTAAGAGTGGTTGTGAAGCTTTTCACAGTGATGAACATTGTTGTAGGAACCATTTTAACAACCCACAAACTTGTAAACCTACTGTGTACTCCAAGTTCTTTAAGGATGCTTGTCCTGCAACGTTTACGTTTGCACATGATTCTCCTTCTTTGATTCATCAGTGTTCTTCACCGGGTGAACTTAAGGTTATCTTTTGCCATTGA

Protein sequence

>MS.gene41190.t1

MASSSSSLTNSLLISTFLFLTIFSPSQALIFTFVNNCPYTVWPAIIPNGGFPVLSTGGFELGHFTHLSIPVPDTHWAGRAWARTGCSTTNNKFSCLTGDCGNRLNCNGAGGSPPATLVQFDVHHGNKDFSSYSVSLVDGFNTPLTVTPHEGKGECPVVGCKADLVASCPPVLQHRVPMGHGPVVACKSGCEAFHSDEHCCRNHFNNPQTCKPTVYSKFFKDACPATFTFAHDSPSLIHQCSSPGELKVIFCH