AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062716

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062716.t1 MTR_1g021945 95.582 249 11 0 1 249 1 249 1.01e-164 455
MS.gene062716.t1 MTR_3g111620 74.104 251 62 3 1 249 1 250 1.34e-129 367
MS.gene062716.t1 MTR_8g036215 52.033 246 107 4 1 244 1 237 2.23e-86 256
MS.gene062716.t1 MTR_5g022350 55.372 242 96 6 7 245 11 243 1.12e-83 253
MS.gene062716.t1 MTR_6g079580 52.479 242 103 5 11 245 4 240 1.56e-83 249
MS.gene062716.t1 MTR_4g073720 54.043 235 100 4 12 244 11 239 4.70e-83 252
MS.gene062716.t1 MTR_8g056820 51.383 253 115 5 3 249 1 251 2.30e-82 247
MS.gene062716.t1 MTR_8g107140 52.869 244 105 4 9 246 7 246 2.38e-81 244
MS.gene062716.t1 MTR_8g037890 52.459 244 106 5 9 248 13 250 1.00e-80 245
MS.gene062716.t1 MTR_8g075510 53.689 244 101 6 8 248 13 247 5.50e-80 241
MS.gene062716.t1 MTR_1g025420 55.230 239 95 7 16 244 9 245 1.26e-79 243
MS.gene062716.t1 MTR_1g025420 54.583 240 100 7 11 244 9 245 1.36e-79 240
MS.gene062716.t1 MTR_3g114030 55.702 228 93 6 25 246 6 231 2.68e-79 239
MS.gene062716.t1 MTR_1g062590 55.983 234 96 6 16 245 12 242 3.22e-79 238
MS.gene062716.t1 MTR_8g075550 54.812 239 96 5 19 248 22 257 1.04e-78 239
MS.gene062716.t1 MTR_8g075510 53.750 240 99 6 8 244 13 243 3.64e-78 238
MS.gene062716.t1 MTR_4g073730 52.459 244 106 6 10 245 7 248 2.34e-76 233
MS.gene062716.t1 MTR_4g073730 52.459 244 106 6 10 245 7 248 5.65e-76 233
MS.gene062716.t1 MTR_2g067980 51.220 246 111 6 7 245 4 247 3.63e-75 246
MS.gene062716.t1 MTR_5g022310 49.402 251 114 6 10 249 7 255 4.01e-75 229
MS.gene062716.t1 MTR_1g062660 51.429 245 111 7 6 246 3 243 4.92e-75 228
MS.gene062716.t1 MTR_5g022310 49.402 251 114 6 10 249 7 255 8.67e-75 229
MS.gene062716.t1 MTR_1g062340 51.852 243 109 7 8 246 5 243 1.60e-74 227
MS.gene062716.t1 MTR_1g062350 52.340 235 105 6 16 246 12 243 1.86e-74 226
MS.gene062716.t1 MTR_2g069660 53.333 240 105 6 10 245 7 243 2.69e-74 226
MS.gene062716.t1 MTR_5g059200 52.018 223 99 2 24 246 27 241 2.78e-74 228
MS.gene062716.t1 MTR_7g102380 52.915 223 98 6 28 246 23 242 5.01e-74 225
MS.gene062716.t1 MTR_1g062370 51.489 235 107 6 16 246 12 243 3.12e-73 223
MS.gene062716.t1 MTR_2g068030 50.226 221 104 5 28 244 25 243 4.83e-73 223
MS.gene062716.t1 MTR_1g062630 52.766 235 104 6 16 246 12 243 7.89e-73 223
MS.gene062716.t1 MTR_7g062610 48.000 225 115 2 22 245 19 242 5.13e-69 213
MS.gene062716.t1 MTR_3g068015 45.344 247 124 4 9 245 3 248 1.17e-68 212
MS.gene062716.t1 MTR_6g009480 45.122 246 126 7 9 247 11 254 1.08e-66 207
MS.gene062716.t1 MTR_2g068655 46.696 227 115 5 23 245 28 252 8.63e-66 207
MS.gene062716.t1 MTR_1g062690 45.957 235 98 5 16 247 12 220 2.06e-60 190
MS.gene062716.t1 MTR_4g063630 42.083 240 123 8 15 244 18 251 5.69e-56 179
MS.gene062716.t1 MTR_5g010635 42.795 229 107 8 23 248 21 228 2.61e-52 170
MS.gene062716.t1 MTR_5g010640 39.516 248 123 8 8 245 6 236 8.49e-48 158
MS.gene062716.t1 MTR_8g096900 39.241 237 121 7 11 245 33 248 7.60e-47 156
MS.gene062716.t1 MTR_2g067990 42.534 221 97 9 28 245 29 222 2.21e-44 159
MS.gene062716.t1 MTR_5g022350 53.595 153 62 5 7 156 76 222 4.69e-43 145
MS.gene062716.t1 MTR_8g089020 50.382 131 60 1 107 237 31 156 2.06e-42 142
MS.gene062716.t1 MTR_2g067970 43.386 189 96 7 68 249 2 186 1.26e-38 143
MS.gene062716.t1 MTR_5g023850 35.081 248 115 9 10 249 11 220 6.53e-38 132
MS.gene062716.t1 MTR_2g063150 31.381 239 151 7 9 238 11 245 5.10e-32 118
MS.gene062716.t1 MTR_7g076360 35.484 217 74 5 23 237 5 157 3.36e-30 111
MS.gene062716.t1 MTR_1g062390 32.377 244 142 7 11 245 9 238 5.42e-30 112
MS.gene062716.t1 MTR_8g096910 45.113 133 63 3 10 141 6 129 1.05e-29 110
MS.gene062716.t1 MTR_8g096920 32.143 224 85 6 23 245 16 173 1.27e-26 102
MS.gene062716.t1 MTR_3g081550 43.750 96 49 3 109 201 16 109 5.32e-14 67.0
MS.gene062716.t1 MTR_1g062640 34.839 155 76 7 21 166 8 146 9.22e-13 64.7
MS.gene062716.t1 MTR_5g068670 47.561 82 38 3 63 143 12 89 7.65e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062716.t1 AT1G75030 67.917 240 73 3 11 248 9 246 6.74e-115 329
MS.gene062716.t1 AT1G75050 67.647 238 71 4 11 245 9 243 4.09e-111 320
MS.gene062716.t1 AT1G75040 63.830 235 81 3 11 245 9 239 3.44e-105 304
MS.gene062716.t1 AT1G19320 60.669 239 88 4 11 244 9 246 1.49e-95 280
MS.gene062716.t1 AT4G38660 57.676 241 94 4 11 249 15 249 6.99e-90 269
MS.gene062716.t1 AT4G38660 58.723 235 87 5 17 249 1 227 1.08e-87 263
MS.gene062716.t1 AT1G73620 54.626 227 96 3 23 246 39 261 2.87e-83 249
MS.gene062716.t1 AT4G24180 55.510 245 97 7 9 246 18 257 7.33e-82 246
MS.gene062716.t1 AT4G24180 55.510 245 97 7 9 246 18 257 1.26e-81 247
MS.gene062716.t1 AT1G77700 51.867 241 105 4 6 244 40 271 4.04e-81 246
MS.gene062716.t1 AT1G77700 51.867 241 105 4 6 244 40 271 4.11e-81 246
MS.gene062716.t1 AT4G24180 53.968 252 105 7 2 246 5 252 8.53e-81 243
MS.gene062716.t1 AT4G24180 53.968 252 105 7 2 246 5 252 2.22e-80 244
MS.gene062716.t1 AT1G75800 53.755 253 104 8 1 245 1 248 4.14e-80 244
MS.gene062716.t1 AT1G18250 53.744 227 98 3 23 246 18 240 7.42e-80 240
MS.gene062716.t1 AT1G77700 53.846 221 94 2 24 244 87 299 1.16e-79 244
MS.gene062716.t1 AT1G18250 52.863 227 100 3 23 246 19 241 3.53e-79 239
MS.gene062716.t1 AT5G24620 53.814 236 100 4 18 245 17 251 1.15e-78 243
MS.gene062716.t1 AT5G24620 53.814 236 100 4 18 245 72 306 2.24e-78 244
MS.gene062716.t1 AT5G24620 53.814 236 100 4 18 245 93 327 3.91e-78 244
MS.gene062716.t1 AT1G20030 56.388 227 89 6 28 246 5 229 6.97e-78 237
MS.gene062716.t1 AT1G20030 56.388 227 89 6 28 246 22 246 8.39e-78 238
MS.gene062716.t1 AT2G17860 55.645 248 97 8 10 248 9 252 1.94e-77 234
MS.gene062716.t1 AT4G36010 54.506 233 94 6 24 246 21 251 7.14e-75 229
MS.gene062716.t1 AT4G36010 54.506 233 94 6 24 246 21 251 7.14e-75 229
MS.gene062716.t1 AT4G24180 54.585 229 92 7 9 230 18 241 8.65e-73 225
MS.gene062716.t1 AT4G24180 52.966 236 100 7 2 230 5 236 1.11e-71 222
MS.gene062716.t1 AT5G40020 45.082 244 125 5 12 247 8 250 3.17e-69 214
MS.gene062716.t1 AT4G38670 49.796 245 112 8 10 245 5 247 3.84e-69 214
MS.gene062716.t1 AT4G38670 49.796 245 112 8 10 245 5 247 3.84e-69 214
MS.gene062716.t1 AT4G38670 52.444 225 98 7 28 245 9 231 1.16e-67 210
MS.gene062716.t1 AT5G02140 45.833 240 127 3 8 245 3 241 1.52e-65 204
MS.gene062716.t1 AT5G02140 45.679 243 129 3 8 248 3 244 3.26e-65 205
MS.gene062716.t1 AT5G38280 45.565 248 123 8 10 249 11 254 3.27e-60 201
MS.gene062716.t1 AT5G38280 45.565 248 123 8 10 249 11 254 3.33e-60 201
MS.gene062716.t1 AT2G28790 43.277 238 125 6 16 244 12 248 1.43e-56 181
MS.gene062716.t1 AT2G28790 43.750 240 121 7 16 244 37 273 2.04e-56 181
MS.gene062716.t1 AT4G11650 43.913 230 108 6 17 245 14 223 2.25e-56 181
MS.gene062716.t1 AT4G18253 41.463 246 132 5 7 249 6 242 4.85e-55 177
MS.gene062716.t1 AT4G36000 56.000 150 57 4 101 249 1 142 9.57e-49 160
MS.gene062716.t1 AT4G36000 56.000 150 57 4 101 249 1 142 9.57e-49 160
MS.gene062716.t1 AT1G70250 40.948 232 120 7 28 249 147 371 2.78e-48 170
MS.gene062716.t1 AT4G18250 40.249 241 134 6 10 247 41 274 1.04e-42 154
MS.gene062716.t1 AT5G24620 54.167 120 48 2 133 245 15 134 8.70e-35 126
MS.gene062716.t1 AT2G24810 63.636 77 28 0 168 244 116 192 2.78e-26 101

Find 95 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGGTTGGTCAGGACGCTTC+TGG 0.127184 1.4:-2531125 MS.gene062716:CDS
ATTTGGCCGGGAACTCTTTC+AGG 0.233483 1.4:-2531222 MS.gene062716:CDS
TTCAACATGTTCCTCTTATT+AGG 0.273557 1.4:-2531387 MS.gene062716:intron
CGGGAACTCTTTCAGGCAAC+GGG 0.293795 1.4:-2531215 MS.gene062716:CDS
TCAAGCACTTGCACGTGTTC+TGG 0.293907 1.4:-2530649 MS.gene062716:CDS
CATTGGGGCAAAACGTGATA+TGG 0.343492 1.4:+2530617 None:intergenic
GGTCAGGACGCTTCTGGGCT+CGG 0.343568 1.4:-2531119 MS.gene062716:CDS
GAAGTCCTTGCCACCATTAC+CGG 0.358634 1.4:+2530972 None:intergenic
ACATTGGCAGAATTCACTAT+TGG 0.360199 1.4:-2531000 MS.gene062716:CDS
CCGTTGCCTGAAAGAGTTCC+CGG 0.373895 1.4:+2531216 None:intergenic
ACCAATGCAGTTACACCATT+TGG 0.377375 1.4:-2531239 MS.gene062716:CDS
GTGAATTCTGCCAATGTTAC+CGG 0.381859 1.4:+2531006 None:intergenic
GGGTTGGTCAGGACGCTTCT+GGG 0.383926 1.4:-2531124 MS.gene062716:CDS
GGCAACGGGGCTGCAATTCT+CGG 0.400439 1.4:-2531201 MS.gene062716:CDS
GTGGATGTCCGGCAGAGCTT+AGG 0.403966 1.4:-2530846 MS.gene062716:CDS
GGGTGTGTTGCGGACTTGAA+TGG 0.414444 1.4:-2530868 MS.gene062716:CDS
GAACATGTTGAAGAGAAGAA+AGG 0.426997 1.4:+2531398 None:intergenic
GGCGTGTAAGAGCGCGTGTT+TGG 0.435818 1.4:-2530800 MS.gene062716:CDS
GGACGCTTCTGGGCTCGGAC+TGG 0.446805 1.4:-2531114 MS.gene062716:CDS
ACTGCACCATCGGGTTGGTC+AGG 0.452166 1.4:-2531135 MS.gene062716:CDS
TATGATCATGATGATCCATT+GGG 0.460244 1.4:+2530601 None:intergenic
GCAAGCTTTCTTAAATATCT+CGG 0.462492 1.4:+2530705 None:intergenic
GCAAAACGTGATATGGTAAT+CGG 0.467360 1.4:+2530624 None:intergenic
TGGATGTCCGGCAGAGCTTA+GGG 0.471726 1.4:-2530845 MS.gene062716:CDS
CGCGTGTTTGGCGTTCAATA+CGG 0.474969 1.4:-2530788 MS.gene062716:CDS
ACAATGTAGGCATGGGTGTA+CGG 0.477021 1.4:-2530924 MS.gene062716:CDS
ACCGGTGATTGTCAATATGC+GGG 0.477080 1.4:-2530889 MS.gene062716:CDS
CCGGGAACTCTTTCAGGCAA+CGG 0.481046 1.4:-2531216 MS.gene062716:CDS
AATGTTGTTTACCTAATAAG+AGG 0.483808 1.4:+2531376 None:intergenic
TAGGGTGAACAGTGCAGGTT+CGG 0.484236 1.4:-2530827 MS.gene062716:CDS
CAATGTAGGCATGGGTGTAC+GGG 0.485572 1.4:-2530923 MS.gene062716:CDS
TGTGTCACAGGAGATTGTTC+CGG 0.487338 1.4:-2531060 MS.gene062716:CDS
CTATGATCATGATGATCCAT+TGG 0.489037 1.4:+2530600 None:intergenic
AGTTAGTTTGACTGCACCAT+CGG 0.493248 1.4:-2531145 MS.gene062716:CDS
ACTATTGGATCAGCCGGTAA+TGG 0.496770 1.4:-2530985 MS.gene062716:CDS
TTGTGGCGTGGCATGTTGGC+CGG 0.499950 1.4:+2530750 None:intergenic
TGAAAGAGTTCCCGGCCAAA+TGG 0.505042 1.4:+2531224 None:intergenic
GAGCTTAGGGTGAACAGTGC+AGG 0.505667 1.4:-2530832 MS.gene062716:CDS
CTGTTCACCCTAAGCTCTGC+CGG 0.506624 1.4:+2530838 None:intergenic
ATGCAGTTACACCATTTGGC+CGG 0.506869 1.4:-2531235 MS.gene062716:CDS
CGGACTGGTTGCGAATTTGA+CGG 0.511038 1.4:-2531099 MS.gene062716:CDS
GTTAGTTTGACTGCACCATC+GGG 0.514040 1.4:-2531144 MS.gene062716:CDS
TAAGCATAACTGTAAGCAGT+TGG 0.515449 1.4:+2530682 None:intergenic
TGCAGTTACACCATTTGGCC+GGG 0.515490 1.4:-2531234 MS.gene062716:CDS
GAACAGTGCAGGTTCGGTGG+TGG 0.517897 1.4:-2530821 MS.gene062716:CDS
GGCAATGGTAAATGTGTCAC+AGG 0.521889 1.4:-2531072 MS.gene062716:CDS
AGTCAAACTAACTGATGTAC+CGG 0.522716 1.4:+2531155 None:intergenic
AAGCATAACTGTAAGCAGTT+GGG 0.532279 1.4:+2530683 None:intergenic
CGACGGTTACAATGTAGGCA+TGG 0.532942 1.4:-2530932 MS.gene062716:CDS
GGACTTCTACGATGTGAGCT+TGG 0.532975 1.4:-2530956 MS.gene062716:CDS
GAATTCACTATTGGATCAGC+CGG 0.534190 1.4:-2530991 MS.gene062716:CDS
GGACTTGAATGGTGGATGTC+CGG 0.536818 1.4:-2530857 MS.gene062716:CDS
GGTTGCGAATTTGACGGTGC+AGG 0.549983 1.4:-2531093 MS.gene062716:CDS
TCCGATACAGTTTAGTCCAC+CGG 0.550929 1.4:+2531041 None:intergenic
GTAACTGCATTGGTTGTGTA+TGG 0.552667 1.4:+2531248 None:intergenic
GAATTTGACGGTGCAGGCAA+TGG 0.556474 1.4:-2531087 MS.gene062716:CDS
TTGGTCGACGGTTACAATGT+AGG 0.559348 1.4:-2530937 MS.gene062716:CDS
CCCCGCATATTGACAATCAC+CGG 0.568073 1.4:+2530888 None:intergenic
GACGGTTACAATGTAGGCAT+GGG 0.570839 1.4:-2530931 MS.gene062716:CDS
TACGATGTGAGCTTGGTCGA+CGG 0.571799 1.4:-2530949 MS.gene062716:CDS
ATGTTTGTGGCGTGGCATGT+TGG 0.573358 1.4:+2530746 None:intergenic
CACCGGTGATTGTCAATATG+CGG 0.583056 1.4:-2530890 MS.gene062716:CDS
TCCGGTGGACTAAACTGTAT+CGG 0.584625 1.4:-2531042 MS.gene062716:CDS
GTTTGACTGCACCATCGGGT+TGG 0.589766 1.4:-2531140 MS.gene062716:CDS
TCAATATGCGGGGTGTGTTG+CGG 0.592968 1.4:-2530878 MS.gene062716:CDS
ATGGGTGTACGGGCTAGAGG+AGG 0.595042 1.4:-2530913 MS.gene062716:CDS
GCCAAATGGTGTAACTGCAT+TGG 0.596575 1.4:+2531238 None:intergenic
GTCAAACTAACTGATGTACC+GGG 0.599721 1.4:+2531156 None:intergenic
GTACGGGCTAGAGGAGGCAC+CGG 0.600853 1.4:-2530907 MS.gene062716:CDS
GGGGCTGCAATTCTCGGTCA+AGG 0.600916 1.4:-2531195 MS.gene062716:CDS
GGCATGGGTGTACGGGCTAG+AGG 0.603450 1.4:-2530916 MS.gene062716:CDS
TGTGTTGCGGACTTGAATGG+TGG 0.603671 1.4:-2530865 MS.gene062716:CDS
AGGTACCCCACCGGTAACAT+TGG 0.604332 1.4:-2531016 MS.gene062716:CDS
GGTGAACAGTGCAGGTTCGG+TGG 0.604516 1.4:-2530824 MS.gene062716:CDS
ATTGGATCAGCCGGTAATGG+TGG 0.605464 1.4:-2530982 MS.gene062716:CDS
AGGCAATTTAGCATCTGCTA+CGG 0.606331 1.4:-2531277 MS.gene062716:CDS
AATTCTGCCAATGTTACCGG+TGG 0.609607 1.4:+2531009 None:intergenic
ATCAGCCGGTAATGGTGGCA+AGG 0.611295 1.4:-2530977 MS.gene062716:CDS
TTCTGCCAATGTTACCGGTG+GGG 0.629018 1.4:+2531011 None:intergenic
GTCACAGGAGATTGTTCCGG+TGG 0.632624 1.4:-2531057 MS.gene062716:CDS
ATTCTGCCAATGTTACCGGT+GGG 0.634739 1.4:+2531010 None:intergenic
TGTGTGGTCGAACATGTTTG+TGG 0.637601 1.4:+2530733 None:intergenic
GGACTAAACTGTATCGGAGG+AGG 0.643072 1.4:-2531036 MS.gene062716:CDS
AAGCGTCCTGACCAACCCGA+TGG 0.645342 1.4:+2531129 None:intergenic
AAATATCTCGGAATAATGTG+TGG 0.647804 1.4:+2530717 None:intergenic
GGGAACTCTTTCAGGCAACG+GGG 0.664283 1.4:-2531214 MS.gene062716:CDS
GTGTTTGGCGTTCAATACGG+AGG 0.667596 1.4:-2530785 MS.gene062716:CDS
GGTCGAACATGTTTGTGGCG+TGG 0.670223 1.4:+2530738 None:intergenic
CCGGTGATTGTCAATATGCG+GGG 0.674634 1.4:-2530888 MS.gene062716:CDS
GCTGCAATTCTCGGTCAAGG+TGG 0.678285 1.4:-2531192 MS.gene062716:CDS
TCAAACTAACTGATGTACCG+GGG 0.689044 1.4:+2531157 None:intergenic
TATCGGAGGAGGTACCCCAC+CGG 0.691570 1.4:-2531025 MS.gene062716:CDS
GGTGGACTAAACTGTATCGG+AGG 0.699134 1.4:-2531039 MS.gene062716:CDS
ATGATCATGATGATCCATTG+GGG 0.705650 1.4:+2530602 None:intergenic
CAAACTAACTGATGTACCGG+GGG 0.713886 1.4:+2531158 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGATTTTTAAAAAGACT+AGG + chr1.4:2530681-2530700 None:intergenic 15.0%
!! AATGTTGTTTACCTAATAAG+AGG + chr1.4:2530656-2530675 None:intergenic 25.0%
!!! TGCATGAATATATTTTTTGC+AGG - chr1.4:2530732-2530751 MS.gene062716:CDS 25.0%
AAATATCTCGGAATAATGTG+TGG + chr1.4:2531315-2531334 None:intergenic 30.0%
GCAAGCTTTCTTAAATATCT+CGG + chr1.4:2531327-2531346 None:intergenic 30.0%
TTCAACATGTTCCTCTTATT+AGG - chr1.4:2530642-2530661 MS.gene062716:CDS 30.0%
! TTGAAGAGAAGAAAGGTTTT+CGG + chr1.4:2530627-2530646 None:intergenic 30.0%
AAGCATAACTGTAAGCAGTT+GGG + chr1.4:2531349-2531368 None:intergenic 35.0%
ACATTGGCAGAATTCACTAT+TGG - chr1.4:2531029-2531048 MS.gene062716:CDS 35.0%
AGTCAAACTAACTGATGTAC+CGG + chr1.4:2530877-2530896 None:intergenic 35.0%
GAACATGTTGAAGAGAAGAA+AGG + chr1.4:2530634-2530653 None:intergenic 35.0%
GCAAAACGTGATATGGTAAT+CGG + chr1.4:2531408-2531427 None:intergenic 35.0%
TAAGCATAACTGTAAGCAGT+TGG + chr1.4:2531350-2531369 None:intergenic 35.0%
! AGAAGAAAGGTTTTCGGATT+TGG + chr1.4:2530621-2530640 None:intergenic 35.0%
ACCAATGCAGTTACACCATT+TGG - chr1.4:2530790-2530809 MS.gene062716:CDS 40.0%
AGGCAATTTAGCATCTGCTA+CGG - chr1.4:2530752-2530771 MS.gene062716:CDS 40.0%
GAATTCACTATTGGATCAGC+CGG - chr1.4:2531038-2531057 MS.gene062716:CDS 40.0%
GTCAAACTAACTGATGTACC+GGG + chr1.4:2530876-2530895 None:intergenic 40.0%
GTGAATTCTGCCAATGTTAC+CGG + chr1.4:2531026-2531045 None:intergenic 40.0%
TCAAACTAACTGATGTACCG+GGG + chr1.4:2530875-2530894 None:intergenic 40.0%
! AGTTAGTTTGACTGCACCAT+CGG - chr1.4:2530884-2530903 MS.gene062716:CDS 40.0%
! GGTTTTCGGATTTGGATTCA+AGG + chr1.4:2530613-2530632 None:intergenic 40.0%
!! GTAACTGCATTGGTTGTGTA+TGG + chr1.4:2530784-2530803 None:intergenic 40.0%
AATTCTGCCAATGTTACCGG+TGG + chr1.4:2531023-2531042 None:intergenic 45.0%
ACAATGTAGGCATGGGTGTA+CGG - chr1.4:2531105-2531124 MS.gene062716:CDS 45.0%
ACCGGTGATTGTCAATATGC+GGG - chr1.4:2531140-2531159 MS.gene062716:CDS 45.0%
ACTATTGGATCAGCCGGTAA+TGG - chr1.4:2531044-2531063 MS.gene062716:CDS 45.0%
ATGCAGTTACACCATTTGGC+CGG - chr1.4:2530794-2530813 MS.gene062716:CDS 45.0%
ATTCTGCCAATGTTACCGGT+GGG + chr1.4:2531022-2531041 None:intergenic 45.0%
CAAACTAACTGATGTACCGG+GGG + chr1.4:2530874-2530893 None:intergenic 45.0%
CACCGGTGATTGTCAATATG+CGG - chr1.4:2531139-2531158 MS.gene062716:CDS 45.0%
CATTGGGGCAAAACGTGATA+TGG + chr1.4:2531415-2531434 None:intergenic 45.0%
GACGGTTACAATGTAGGCAT+GGG - chr1.4:2531098-2531117 MS.gene062716:CDS 45.0%
GCCAAATGGTGTAACTGCAT+TGG + chr1.4:2530794-2530813 None:intergenic 45.0%
GGCAATGGTAAATGTGTCAC+AGG - chr1.4:2530957-2530976 MS.gene062716:CDS 45.0%
TCCGATACAGTTTAGTCCAC+CGG + chr1.4:2530991-2531010 None:intergenic 45.0%
TGTGTCACAGGAGATTGTTC+CGG - chr1.4:2530969-2530988 MS.gene062716:CDS 45.0%
TGTGTGGTCGAACATGTTTG+TGG + chr1.4:2531299-2531318 None:intergenic 45.0%
TTGGTCGACGGTTACAATGT+AGG - chr1.4:2531092-2531111 MS.gene062716:CDS 45.0%
! CATATCACGTTTTGCCCCAA+TGG - chr1.4:2531413-2531432 MS.gene062716:CDS 45.0%
! GTTAGTTTGACTGCACCATC+GGG - chr1.4:2530885-2530904 MS.gene062716:CDS 45.0%
!! TCCGGTGGACTAAACTGTAT+CGG - chr1.4:2530987-2531006 MS.gene062716:CDS 45.0%
ATGTTTGTGGCGTGGCATGT+TGG + chr1.4:2531286-2531305 None:intergenic 50.0%
ATTTGGCCGGGAACTCTTTC+AGG - chr1.4:2530807-2530826 MS.gene062716:CDS 50.0%
CAATGTAGGCATGGGTGTAC+GGG - chr1.4:2531106-2531125 MS.gene062716:CDS 50.0%
CCCCGCATATTGACAATCAC+CGG + chr1.4:2531144-2531163 None:intergenic 50.0%
CCGGTGATTGTCAATATGCG+GGG - chr1.4:2531141-2531160 MS.gene062716:CDS 50.0%
CGACGGTTACAATGTAGGCA+TGG - chr1.4:2531097-2531116 MS.gene062716:CDS 50.0%
CGCGTGTTTGGCGTTCAATA+CGG - chr1.4:2531241-2531260 MS.gene062716:CDS 50.0%
GAAGTCCTTGCCACCATTAC+CGG + chr1.4:2531060-2531079 None:intergenic 50.0%
GAATTTGACGGTGCAGGCAA+TGG - chr1.4:2530942-2530961 MS.gene062716:CDS 50.0%
GGACTAAACTGTATCGGAGG+AGG - chr1.4:2530993-2531012 MS.gene062716:CDS 50.0%
GGACTTCTACGATGTGAGCT+TGG - chr1.4:2531073-2531092 MS.gene062716:CDS 50.0%
GTGTTTGGCGTTCAATACGG+AGG - chr1.4:2531244-2531263 MS.gene062716:CDS 50.0%
TACGATGTGAGCTTGGTCGA+CGG - chr1.4:2531080-2531099 MS.gene062716:CDS 50.0%
TAGGGTGAACAGTGCAGGTT+CGG - chr1.4:2531202-2531221 MS.gene062716:CDS 50.0%
TCAAGCACTTGCACGTGTTC+TGG - chr1.4:2531380-2531399 MS.gene062716:intron 50.0%
TCAATATGCGGGGTGTGTTG+CGG - chr1.4:2531151-2531170 MS.gene062716:CDS 50.0%
TGAAAGAGTTCCCGGCCAAA+TGG + chr1.4:2530808-2530827 None:intergenic 50.0%
TGCAGTTACACCATTTGGCC+GGG - chr1.4:2530795-2530814 MS.gene062716:CDS 50.0%
TGTGTTGCGGACTTGAATGG+TGG - chr1.4:2531164-2531183 MS.gene062716:CDS 50.0%
TTCTGCCAATGTTACCGGTG+GGG + chr1.4:2531021-2531040 None:intergenic 50.0%
! ATTGGATCAGCCGGTAATGG+TGG - chr1.4:2531047-2531066 MS.gene062716:CDS 50.0%
!! CGGACTGGTTGCGAATTTGA+CGG - chr1.4:2530930-2530949 MS.gene062716:CDS 50.0%
!! GGACTTGAATGGTGGATGTC+CGG - chr1.4:2531172-2531191 MS.gene062716:CDS 50.0%
!! GGTGGACTAAACTGTATCGG+AGG - chr1.4:2530990-2531009 MS.gene062716:CDS 50.0%
!!! ACGGAGGAGTTTTGTTGCAC+CGG - chr1.4:2531260-2531279 MS.gene062716:CDS 50.0%
AGGTACCCCACCGGTAACAT+TGG - chr1.4:2531013-2531032 MS.gene062716:CDS 55.0%
CCGGGAACTCTTTCAGGCAA+CGG - chr1.4:2530813-2530832 MS.gene062716:CDS 55.0%
CCGTTGCCTGAAAGAGTTCC+CGG + chr1.4:2530816-2530835 None:intergenic 55.0%
CGGGAACTCTTTCAGGCAAC+GGG - chr1.4:2530814-2530833 MS.gene062716:CDS 55.0%
CTGTTCACCCTAAGCTCTGC+CGG + chr1.4:2531194-2531213 None:intergenic 55.0%
GAGCTTAGGGTGAACAGTGC+AGG - chr1.4:2531197-2531216 MS.gene062716:CDS 55.0%
GCTGCAATTCTCGGTCAAGG+TGG - chr1.4:2530837-2530856 MS.gene062716:CDS 55.0%
GGGAACTCTTTCAGGCAACG+GGG - chr1.4:2530815-2530834 MS.gene062716:CDS 55.0%
GGGTGTGTTGCGGACTTGAA+TGG - chr1.4:2531161-2531180 MS.gene062716:CDS 55.0%
GGTCGAACATGTTTGTGGCG+TGG + chr1.4:2531294-2531313 None:intergenic 55.0%
GGTTGCGAATTTGACGGTGC+AGG - chr1.4:2530936-2530955 MS.gene062716:CDS 55.0%
GTCACAGGAGATTGTTCCGG+TGG - chr1.4:2530972-2530991 MS.gene062716:CDS 55.0%
GTTTGACTGCACCATCGGGT+TGG - chr1.4:2530889-2530908 MS.gene062716:CDS 55.0%
TGGATGTCCGGCAGAGCTTA+GGG - chr1.4:2531184-2531203 MS.gene062716:CDS 55.0%
! ATCAGCCGGTAATGGTGGCA+AGG - chr1.4:2531052-2531071 MS.gene062716:CDS 55.0%
!!! GGTGGTTTTTTGCTAGCCCC+CGG - chr1.4:2530855-2530874 MS.gene062716:CDS 55.0%
AAGCGTCCTGACCAACCCGA+TGG + chr1.4:2530903-2530922 None:intergenic 60.0%
GGCAACGGGGCTGCAATTCT+CGG - chr1.4:2530828-2530847 MS.gene062716:CDS 60.0%
GGGGCTGCAATTCTCGGTCA+AGG - chr1.4:2530834-2530853 MS.gene062716:CDS 60.0%
GGTGAACAGTGCAGGTTCGG+TGG - chr1.4:2531205-2531224 MS.gene062716:CDS 60.0%
GTGGATGTCCGGCAGAGCTT+AGG - chr1.4:2531183-2531202 MS.gene062716:CDS 60.0%
TTGTGGCGTGGCATGTTGGC+CGG + chr1.4:2531282-2531301 None:intergenic 60.0%
! GGCGTGTAAGAGCGCGTGTT+TGG - chr1.4:2531229-2531248 MS.gene062716:CDS 60.0%
!! ACTGCACCATCGGGTTGGTC+AGG - chr1.4:2530894-2530913 MS.gene062716:CDS 60.0%
!! ATGGGTGTACGGGCTAGAGG+AGG - chr1.4:2531116-2531135 MS.gene062716:CDS 60.0%
!! GAACAGTGCAGGTTCGGTGG+TGG - chr1.4:2531208-2531227 MS.gene062716:CDS 60.0%
!! GGGTTGGTCAGGACGCTTCT+GGG - chr1.4:2530905-2530924 MS.gene062716:CDS 60.0%
!! TATCGGAGGAGGTACCCCAC+CGG - chr1.4:2531004-2531023 MS.gene062716:CDS 60.0%
GGTCAGGACGCTTCTGGGCT+CGG - chr1.4:2530910-2530929 MS.gene062716:CDS 65.0%
!! CGGGTTGGTCAGGACGCTTC+TGG - chr1.4:2530904-2530923 MS.gene062716:CDS 65.0%
!! GGCATGGGTGTACGGGCTAG+AGG - chr1.4:2531113-2531132 MS.gene062716:CDS 65.0%
!! GTACGGGCTAGAGGAGGCAC+CGG - chr1.4:2531122-2531141 MS.gene062716:CDS 65.0%
GGACGCTTCTGGGCTCGGAC+TGG - chr1.4:2530915-2530934 MS.gene062716:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 2530606 2531445 2530606 ID=MS.gene062716
chr1.4 mRNA 2530606 2531445 2530606 ID=MS.gene062716.t1;Parent=MS.gene062716
chr1.4 exon 2531388 2531445 2531388 ID=MS.gene062716.t1.exon1;Parent=MS.gene062716.t1
chr1.4 CDS 2531388 2531445 2531388 ID=cds.MS.gene062716.t1;Parent=MS.gene062716.t1
chr1.4 exon 2530606 2531297 2530606 ID=MS.gene062716.t1.exon2;Parent=MS.gene062716.t1
chr1.4 CDS 2530606 2531297 2530606 ID=cds.MS.gene062716.t1;Parent=MS.gene062716.t1
Gene Sequence

>MS.gene062716

ATGGCCTTGAATCCAAATCCGAAAACCTTTCTTCTCTTCAACATGTTCCTCTTATTAGGTAAACAACATTTACCTAGTCTTTTTAAAAATCATTTTAATTTTTTTATTTTTATAATTAATAGCTTATGCATGAATATATTTTTTGCAGGCAATTTAGCATCTGCTACGGTGTTTACCATACACAACCAATGCAGTTACACCATTTGGCCGGGAACTCTTTCAGGCAACGGGGCTGCAATTCTCGGTCAAGGTGGTTTTTTGCTAGCCCCCGGTACATCAGTTAGTTTGACTGCACCATCGGGTTGGTCAGGACGCTTCTGGGCTCGGACTGGTTGCGAATTTGACGGTGCAGGCAATGGTAAATGTGTCACAGGAGATTGTTCCGGTGGACTAAACTGTATCGGAGGAGGTACCCCACCGGTAACATTGGCAGAATTCACTATTGGATCAGCCGGTAATGGTGGCAAGGACTTCTACGATGTGAGCTTGGTCGACGGTTACAATGTAGGCATGGGTGTACGGGCTAGAGGAGGCACCGGTGATTGTCAATATGCGGGGTGTGTTGCGGACTTGAATGGTGGATGTCCGGCAGAGCTTAGGGTGAACAGTGCAGGTTCGGTGGTGGCGTGTAAGAGCGCGTGTTTGGCGTTCAATACGGAGGAGTTTTGTTGCACCGGCCAACATGCCACGCCACAAACATGTTCGACCACACATTATTCCGAGATATTTAAGAAAGCTTGCCCAACTGCTTACAGTTATGCTTATGATGATGCTTCAAGCACTTGCACGTGTTCTGGTTCCGATTACCATATCACGTTTTGCCCCAATGGATCATCATGA
Protein sequence

>MS.gene062716.t1

MALNPNPKTFLLFNMFLLLGNLASATVFTIHNQCSYTIWPGTLSGNGAAILGQGGFLLAPGTSVSLTAPSGWSGRFWARTGCEFDGAGNGKCVTGDCSGGLNCIGGGTPPVTLAEFTIGSAGNGGKDFYDVSLVDGYNVGMGVRARGGTGDCQYAGCVADLNGGCPAELRVNSAGSVVACKSACLAFNTEEFCCTGQHATPQTCSTTHYSEIFKKACPTAYSYAYDDASSTCTCSGSDYHITFCPNGSS