AlfalfaGEDB | MS.gene062716

M. sativa cultivar XinJiangDaYe / MS.gene062716

KEGG: -
GO: -
PFAM: PF00314.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062716.t1	XP_013466052.1	95.6	249	11	0	1	249	1	249	7.90E-136	493

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062716.t1	Q5DWG1	66.2	234	72	3	15	246	11	239	1.7e-86	320.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062716.t1	A0A072VEQ3	95.6	249	11	0	1	249	1	249	5.7e-136	493.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene00249	MS.gene062716	PPI
MS.gene003526	MS.gene062716	PPI
MS.gene004055	MS.gene062716	PPI
MS.gene016652	MS.gene062716	PPI
MS.gene019285	MS.gene062716	PPI
MS.gene040126	MS.gene062716	PPI
MS.gene044112	MS.gene062716	PPI
MS.gene050380	MS.gene062716	PPI
MS.gene056184	MS.gene062716	PPI
MS.gene056186	MS.gene062716	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062716.t1	MTR_1g021945	95.582	249	11	0	1	249	1	249	1.01e-164	455
MS.gene062716.t1	MTR_3g111620	74.104	251	62	3	1	249	1	250	1.34e-129	367
MS.gene062716.t1	MTR_8g036215	52.033	246	107	4	1	244	1	237	2.23e-86	256
MS.gene062716.t1	MTR_5g022350	55.372	242	96	6	7	245	11	243	1.12e-83	253
MS.gene062716.t1	MTR_6g079580	52.479	242	103	5	11	245	4	240	1.56e-83	249
MS.gene062716.t1	MTR_4g073720	54.043	235	100	4	12	244	11	239	4.70e-83	252
MS.gene062716.t1	MTR_8g056820	51.383	253	115	5	3	249	1	251	2.30e-82	247
MS.gene062716.t1	MTR_8g107140	52.869	244	105	4	9	246	7	246	2.38e-81	244
MS.gene062716.t1	MTR_8g037890	52.459	244	106	5	9	248	13	250	1.00e-80	245
MS.gene062716.t1	MTR_8g075510	53.689	244	101	6	8	248	13	247	5.50e-80	241

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062716.t1	AT1G75030	67.917	240	73	3	11	248	9	246	6.74e-115	329
MS.gene062716.t1	AT1G75050	67.647	238	71	4	11	245	9	243	4.09e-111	320
MS.gene062716.t1	AT1G75040	63.830	235	81	3	11	245	9	239	3.44e-105	304
MS.gene062716.t1	AT1G19320	60.669	239	88	4	11	244	9	246	1.49e-95	280
MS.gene062716.t1	AT4G38660	57.676	241	94	4	11	249	15	249	6.99e-90	269
MS.gene062716.t1	AT4G38660	58.723	235	87	5	17	249	1	227	1.08e-87	263
MS.gene062716.t1	AT1G73620	54.626	227	96	3	23	246	39	261	2.87e-83	249
MS.gene062716.t1	AT4G24180	55.510	245	97	7	9	246	18	257	7.33e-82	246
MS.gene062716.t1	AT4G24180	55.510	245	97	7	9	246	18	257	1.26e-81	247
MS.gene062716.t1	AT1G77700	51.867	241	105	4	6	244	40	271	4.04e-81	246

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Find 95 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATATCTCGGAATAATGTG+TGG	0.647804	1.4:+2530717	None:intergenic
AAGCATAACTGTAAGCAGTT+GGG	0.532279	1.4:+2530683	None:intergenic
AAGCGTCCTGACCAACCCGA+TGG	0.645342	1.4:+2531129	None:intergenic
AATGTTGTTTACCTAATAAG+AGG	0.483808	1.4:+2531376	None:intergenic
AATTCTGCCAATGTTACCGG+TGG	0.609607	1.4:+2531009	None:intergenic
ACAATGTAGGCATGGGTGTA+CGG	0.477021	1.4:-2530924	MS.gene062716:CDS
ACATTGGCAGAATTCACTAT+TGG	0.360199	1.4:-2531000	MS.gene062716:CDS
ACCAATGCAGTTACACCATT+TGG	0.377375	1.4:-2531239	MS.gene062716:CDS
ACCGGTGATTGTCAATATGC+GGG	0.477080	1.4:-2530889	MS.gene062716:CDS
ACTATTGGATCAGCCGGTAA+TGG	0.496770	1.4:-2530985	MS.gene062716:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATATCTCGGAATAATGTG+TGG	+	chr1.4:2531315-2531334	None:intergenic	30.0%
GCAAGCTTTCTTAAATATCT+CGG	+	chr1.4:2531327-2531346	None:intergenic	30.0%
TTCAACATGTTCCTCTTATT+AGG	-	chr1.4:2530642-2530661	MS.gene062716:CDS	30.0%
AAGCATAACTGTAAGCAGTT+GGG	+	chr1.4:2531349-2531368	None:intergenic	35.0%
ACATTGGCAGAATTCACTAT+TGG	-	chr1.4:2531029-2531048	MS.gene062716:CDS	35.0%
AGTCAAACTAACTGATGTAC+CGG	+	chr1.4:2530877-2530896	None:intergenic	35.0%
GAACATGTTGAAGAGAAGAA+AGG	+	chr1.4:2530634-2530653	None:intergenic	35.0%
GCAAAACGTGATATGGTAAT+CGG	+	chr1.4:2531408-2531427	None:intergenic	35.0%
TAAGCATAACTGTAAGCAGT+TGG	+	chr1.4:2531350-2531369	None:intergenic	35.0%
ACCAATGCAGTTACACCATT+TGG	-	chr1.4:2530790-2530809	MS.gene062716:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	2530606	2531445	2530606	ID=MS.gene062716
chr1.4	mRNA	2530606	2531445	2530606	ID=MS.gene062716.t1;Parent=MS.gene062716
chr1.4	exon	2531388	2531445	2531388	ID=MS.gene062716.t1.exon1;Parent=MS.gene062716.t1
chr1.4	CDS	2531388	2531445	2531388	ID=cds.MS.gene062716.t1;Parent=MS.gene062716.t1
chr1.4	exon	2530606	2531297	2530606	ID=MS.gene062716.t1.exon2;Parent=MS.gene062716.t1
chr1.4	CDS	2530606	2531297	2530606	ID=cds.MS.gene062716.t1;Parent=MS.gene062716.t1

Gene Sequence

>MS.gene062716

ATGGCCTTGAATCCAAATCCGAAAACCTTTCTTCTCTTCAACATGTTCCTCTTATTAGGTAAACAACATTTACCTAGTCTTTTTAAAAATCATTTTAATTTTTTTATTTTTATAATTAATAGCTTATGCATGAATATATTTTTTGCAGGCAATTTAGCATCTGCTACGGTGTTTACCATACACAACCAATGCAGTTACACCATTTGGCCGGGAACTCTTTCAGGCAACGGGGCTGCAATTCTCGGTCAAGGTGGTTTTTTGCTAGCCCCCGGTACATCAGTTAGTTTGACTGCACCATCGGGTTGGTCAGGACGCTTCTGGGCTCGGACTGGTTGCGAATTTGACGGTGCAGGCAATGGTAAATGTGTCACAGGAGATTGTTCCGGTGGACTAAACTGTATCGGAGGAGGTACCCCACCGGTAACATTGGCAGAATTCACTATTGGATCAGCCGGTAATGGTGGCAAGGACTTCTACGATGTGAGCTTGGTCGACGGTTACAATGTAGGCATGGGTGTACGGGCTAGAGGAGGCACCGGTGATTGTCAATATGCGGGGTGTGTTGCGGACTTGAATGGTGGATGTCCGGCAGAGCTTAGGGTGAACAGTGCAGGTTCGGTGGTGGCGTGTAAGAGCGCGTGTTTGGCGTTCAATACGGAGGAGTTTTGTTGCACCGGCCAACATGCCACGCCACAAACATGTTCGACCACACATTATTCCGAGATATTTAAGAAAGCTTGCCCAACTGCTTACAGTTATGCTTATGATGATGCTTCAAGCACTTGCACGTGTTCTGGTTCCGATTACCATATCACGTTTTGCCCCAATGGATCATCATGA

Protein sequence

>MS.gene062716.t1

MALNPNPKTFLLFNMFLLLGNLASATVFTIHNQCSYTIWPGTLSGNGAAILGQGGFLLAPGTSVSLTAPSGWSGRFWARTGCEFDGAGNGKCVTGDCSGGLNCIGGGTPPVTLAEFTIGSAGNGGKDFYDVSLVDGYNVGMGVRARGGTGDCQYAGCVADLNGGCPAELRVNSAGSVVACKSACLAFNTEEFCCTGQHATPQTCSTTHYSEIFKKACPTAYSYAYDDASSTCTCSGSDYHITFCPNGSS