AlfalfaGEDB | MS.gene93762

M. sativa cultivar XinJiangDaYe / MS.gene93762

KEGG: -
GO: -
PFAM: PF00314.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93762.t1	XP_003631101.1	96.8	250	7	1	1	250	1	249	5.70E-142	513.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93762.t1	P50699	75.8	236	57	0	14	249	7	242	4.9e-110	398.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93762.t1	G7LDC8	96.8	250	7	1	1	250	1	249	4.1e-142	513.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049298	MS.gene93762	0.848189	7.18E-60	-1.69E-46
MS.gene050710	MS.gene93762	0.802549	5.32E-49	-1.69E-46
MS.gene050874	MS.gene93762	0.808773	2.63E-50	-1.69E-46
MS.gene050875	MS.gene93762	0.825452	4.65E-54	-1.69E-46
MS.gene051896	MS.gene93762	0.809793	1.59E-50	-1.69E-46
MS.gene051914	MS.gene93762	0.852137	5.62E-61	-1.69E-46
MS.gene052209	MS.gene93762	0.81348	2.51E-51	-1.69E-46
MS.gene052308	MS.gene93762	0.868142	8.13E-66	-1.69E-46
MS.gene053108	MS.gene93762	0.800123	1.67E-48	-1.69E-46
MS.gene053173	MS.gene93762	0.836923	7.04E-57	-1.69E-46
MS.gene053348	MS.gene93762	0.818943	1.51E-52	-1.69E-46
MS.gene054551	MS.gene93762	0.833837	4.24E-56	-1.69E-46
MS.gene054593	MS.gene93762	0.834632	2.68E-56	-1.69E-46
MS.gene054633	MS.gene93762	0.850981	1.19E-60	-1.69E-46
MS.gene054662	MS.gene93762	0.838912	2.17E-57	-1.69E-46
MS.gene054850	MS.gene93762	0.809253	2.07E-50	-1.69E-46
MS.gene054941	MS.gene93762	0.832105	1.14E-55	-1.69E-46
MS.gene055308	MS.gene93762	0.859889	3.03E-63	-1.69E-46
MS.gene055578	MS.gene93762	0.860106	2.60E-63	-1.69E-46
MS.gene056041	MS.gene93762	0.82557	4.36E-54	-1.69E-46
MS.gene05640	MS.gene93762	0.859131	5.11E-63	-1.69E-46
MS.gene056528	MS.gene93762	0.804497	2.10E-49	-1.69E-46
MS.gene05652	MS.gene93762	0.904854	7.94E-80	-1.69E-46
MS.gene056618	MS.gene93762	0.809712	1.65E-50	-1.69E-46
MS.gene057101	MS.gene93762	0.818952	1.50E-52	-1.69E-46
MS.gene057367	MS.gene93762	0.83271	8.09E-56	-1.69E-46
MS.gene057458	MS.gene93762	0.81375	2.19E-51	-1.69E-46
MS.gene057669	MS.gene93762	0.86192	7.31E-64	-1.69E-46
MS.gene058013	MS.gene93762	0.816158	6.39E-52	-1.69E-46
MS.gene058128	MS.gene93762	0.91022	2.39E-82	-1.69E-46
MS.gene058789	MS.gene93762	0.81513	1.08E-51	-1.69E-46
MS.gene058853	MS.gene93762	0.885503	7.69E-72	-1.69E-46
MS.gene05926	MS.gene93762	0.818746	1.67E-52	-1.69E-46
MS.gene059616	MS.gene93762	0.891997	2.38E-74	-1.69E-46
MS.gene059905	MS.gene93762	0.806364	8.53E-50	-1.69E-46
MS.gene06093	MS.gene93762	0.849504	3.10E-60	-1.69E-46
MS.gene060985	MS.gene93762	0.801521	8.66E-49	-1.69E-46
MS.gene061089	MS.gene93762	0.801556	8.51E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene93762	MS.gene42714	PPI
MS.gene93762	MS.gene021099	PPI
MS.gene93762	MS.gene016652	PPI
MS.gene93762	MS.gene062716	PPI
MS.gene93762	MS.gene68072	PPI
MS.gene93762	MS.gene014545	PPI
MS.gene93762	MS.gene24315	PPI
MS.gene93762	MS.gene26931	PPI
MS.gene93762	MS.gene59476	PPI
MS.gene93762	MS.gene072805	PPI
MS.gene93762	MS.gene56731	PPI
MS.gene93762	MS.gene69123	PPI
MS.gene93762	MS.gene27345	PPI
MS.gene94553	MS.gene93762	PPI
MS.gene93762	MS.gene056184	PPI
MS.gene93762	MS.gene41190	PPI
MS.gene93762	MS.gene64778	PPI
MS.gene93762	MS.gene20542	PPI
MS.gene040126	MS.gene93762	PPI
MS.gene93762	MS.gene019285	PPI
MS.gene93762	MS.gene046990	PPI
MS.gene93762	MS.gene92395	PPI
MS.gene93762	MS.gene45764	PPI
MS.gene93762	MS.gene92397	PPI
MS.gene93762	MS.gene010631	PPI
MS.gene056186	MS.gene93762	PPI
MS.gene93762	MS.gene42360	PPI
MS.gene93762	MS.gene61094	PPI
MS.gene93762	MS.gene050380	PPI
MS.gene93762	MS.gene004055	PPI
MS.gene93762	MS.gene015555	PPI
MS.gene93762	MS.gene92393	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93762.t1	MTR_8g107140	96.800	250	7	1	1	250	1	249	1.70e-179	493
MS.gene93762.t1	MTR_6g079580	69.748	238	71	1	9	246	4	240	1.04e-129	367
MS.gene93762.t1	MTR_8g036215	58.636	220	89	2	27	245	19	237	1.22e-90	267
MS.gene93762.t1	MTR_3g111620	54.626	227	98	3	23	246	22	246	3.71e-88	261
MS.gene93762.t1	MTR_8g075550	54.310	232	98	3	27	250	27	258	5.34e-85	256
MS.gene93762.t1	MTR_4g073720	52.049	244	101	5	4	245	10	239	2.96e-83	252
MS.gene93762.t1	MTR_1g025420	52.893	242	109	4	9	245	4	245	1.80e-82	249
MS.gene93762.t1	MTR_1g025420	52.893	242	109	4	9	245	4	245	2.33e-82	248
MS.gene93762.t1	MTR_8g075510	54.167	240	98	6	10	246	14	244	1.03e-81	245
MS.gene93762.t1	MTR_1g021945	54.148	229	98	3	23	247	21	246	1.76e-81	244
MS.gene93762.t1	MTR_7g062610	50.000	236	112	3	14	246	10	242	5.42e-81	243
MS.gene93762.t1	MTR_5g022350	55.157	223	90	4	26	246	29	243	6.65e-81	245
MS.gene93762.t1	MTR_8g075510	53.975	239	98	6	10	245	14	243	8.02e-81	245
MS.gene93762.t1	MTR_3g068015	49.597	248	115	4	7	246	3	248	1.28e-80	242
MS.gene93762.t1	MTR_8g037890	49.801	251	117	5	1	246	1	247	5.54e-79	241
MS.gene93762.t1	MTR_5g059200	53.097	226	99	4	25	250	26	244	4.08e-77	234
MS.gene93762.t1	MTR_5g022310	50.198	253	117	6	1	246	1	251	5.21e-77	233
MS.gene93762.t1	MTR_5g022310	50.198	253	117	6	1	246	1	251	1.01e-76	234
MS.gene93762.t1	MTR_7g102380	52.679	224	100	5	28	247	21	242	1.63e-76	232
MS.gene93762.t1	MTR_3g114030	50.218	229	109	4	27	250	6	234	9.49e-74	224
MS.gene93762.t1	MTR_1g062590	47.325	243	122	5	8	246	2	242	1.82e-73	224
MS.gene93762.t1	MTR_8g056820	48.374	246	117	5	6	246	7	247	1.98e-73	224
MS.gene93762.t1	MTR_6g009480	44.664	253	132	6	1	247	3	253	3.30e-73	224
MS.gene93762.t1	MTR_2g069660	50.885	226	106	4	25	246	19	243	1.38e-71	219
MS.gene93762.t1	MTR_1g062370	48.117	239	118	5	12	246	6	242	3.23e-71	218
MS.gene93762.t1	MTR_1g062350	47.699	239	119	5	12	246	6	242	2.06e-70	216
MS.gene93762.t1	MTR_1g062340	47.899	238	118	5	13	246	7	242	3.70e-70	216
MS.gene93762.t1	MTR_4g073730	49.794	243	115	6	9	246	8	248	7.08e-70	216
MS.gene93762.t1	MTR_4g073730	49.794	243	115	6	9	246	8	248	1.95e-69	216
MS.gene93762.t1	MTR_1g062660	48.458	227	111	5	26	248	20	244	3.55e-69	213
MS.gene93762.t1	MTR_1g062630	45.528	246	128	5	8	249	2	245	9.62e-69	213
MS.gene93762.t1	MTR_2g068030	46.725	229	115	5	22	245	17	243	6.26e-67	207
MS.gene93762.t1	MTR_2g067980	46.939	245	118	6	9	246	8	247	1.87e-64	216
MS.gene93762.t1	MTR_4g063630	44.958	238	120	6	14	245	19	251	3.98e-63	198
MS.gene93762.t1	MTR_2g068655	44.541	229	120	5	27	250	30	256	1.90e-60	193
MS.gene93762.t1	MTR_1g062690	43.038	237	109	5	12	246	6	218	2.98e-58	184
MS.gene93762.t1	MTR_5g010640	39.130	253	126	8	4	246	2	236	5.54e-50	164
MS.gene93762.t1	MTR_5g010635	41.593	226	107	8	25	246	21	225	4.14e-49	162
MS.gene93762.t1	MTR_2g067970	45.902	183	89	7	71	246	3	182	5.07e-47	167
MS.gene93762.t1	MTR_8g096900	37.200	250	123	12	3	246	27	248	2.86e-45	152
MS.gene93762.t1	MTR_8g089020	48.936	141	69	2	101	241	22	159	1.29e-42	142
MS.gene93762.t1	MTR_5g022350	52.985	134	58	3	24	155	92	222	8.46e-42	142
MS.gene93762.t1	MTR_2g067990	37.946	224	108	9	27	246	26	222	2.29e-40	148
MS.gene93762.t1	MTR_2g063150	34.146	205	127	5	28	228	34	234	4.20e-36	129
MS.gene93762.t1	MTR_5g023850	34.400	250	119	10	7	247	4	217	1.47e-35	126
MS.gene93762.t1	MTR_7g076360	34.123	211	76	4	27	236	7	155	4.32e-30	110
MS.gene93762.t1	MTR_1g062390	30.980	255	150	8	1	246	1	238	1.07e-29	112
MS.gene93762.t1	MTR_8g096910	39.041	146	74	5	4	146	2	135	7.99e-28	105
MS.gene93762.t1	MTR_8g096920	41.600	125	62	4	25	146	16	132	1.56e-24	96.3
MS.gene93762.t1	MTR_3g081550	42.553	94	51	2	112	202	16	109	4.92e-16	72.4
MS.gene93762.t1	MTR_5g068670	48.750	80	37	2	65	142	12	89	4.73e-14	67.4
MS.gene93762.t1	MTR_1g062640	31.507	146	90	4	25	167	10	148	9.55e-14	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93762.t1	AT1G18250	78.414	227	49	0	24	250	18	244	1.49e-135	382
MS.gene93762.t1	AT1G18250	77.876	226	50	0	25	250	18	243	9.58e-135	379
MS.gene93762.t1	AT1G73620	74.779	226	57	0	25	250	39	264	1.81e-130	369
MS.gene93762.t1	AT1G75050	55.882	238	101	3	14	249	11	246	3.05e-89	264
MS.gene93762.t1	AT1G75030	55.947	227	95	3	26	249	22	246	8.08e-86	256
MS.gene93762.t1	AT1G75040	55.645	248	99	6	1	246	1	239	7.90e-85	253
MS.gene93762.t1	AT1G19320	53.386	251	106	5	1	245	1	246	8.61e-83	248
MS.gene93762.t1	AT4G38660	51.304	230	105	3	21	248	1	225	2.53e-81	247
MS.gene93762.t1	AT4G38660	50.000	246	112	4	3	246	9	245	5.75e-81	247
MS.gene93762.t1	AT1G77700	53.712	229	98	5	23	250	55	276	1.86e-79	242
MS.gene93762.t1	AT1G77700	53.680	231	99	5	16	245	76	299	3.16e-79	243
MS.gene93762.t1	AT1G77700	54.911	224	93	5	23	245	55	271	3.40e-79	241
MS.gene93762.t1	AT1G75800	53.333	240	105	5	14	246	9	248	1.18e-78	240
MS.gene93762.t1	AT1G20030	54.667	225	95	5	29	246	4	228	5.12e-77	235
MS.gene93762.t1	AT1G20030	54.667	225	95	5	29	246	21	245	7.16e-77	235
MS.gene93762.t1	AT4G24180	48.438	256	111	6	5	250	16	260	9.33e-76	231
MS.gene93762.t1	AT4G24180	48.988	247	111	5	14	250	14	255	2.97e-75	229
MS.gene93762.t1	AT4G24180	48.810	252	108	6	5	246	16	256	3.71e-75	231
MS.gene93762.t1	AT4G24180	49.383	243	108	5	14	246	14	251	1.54e-74	229
MS.gene93762.t1	AT5G02140	47.280	239	120	4	12	246	5	241	1.41e-73	226
MS.gene93762.t1	AT5G40020	45.902	244	126	3	10	248	8	250	1.50e-73	225
MS.gene93762.t1	AT5G02140	47.280	239	120	4	12	246	5	241	1.86e-73	224
MS.gene93762.t1	AT5G24620	46.718	259	118	5	1	250	9	256	4.58e-73	229
MS.gene93762.t1	AT5G24620	46.718	259	118	5	1	250	64	311	6.44e-73	230
MS.gene93762.t1	AT2G17860	47.755	245	120	5	14	250	9	253	8.46e-73	223
MS.gene93762.t1	AT5G24620	46.718	259	118	5	1	250	85	332	1.31e-72	230
MS.gene93762.t1	AT4G36010	51.082	231	102	5	26	246	21	250	3.69e-72	223
MS.gene93762.t1	AT4G36010	51.082	231	102	5	26	246	21	250	3.69e-72	223
MS.gene93762.t1	AT4G38670	48.387	248	115	6	5	246	7	247	9.92e-70	216
MS.gene93762.t1	AT4G38670	48.387	248	115	6	5	246	7	247	9.92e-70	216
MS.gene93762.t1	AT4G38670	51.339	224	103	5	29	246	8	231	1.92e-68	212
MS.gene93762.t1	AT4G24180	47.257	237	104	6	5	231	16	241	1.95e-66	209
MS.gene93762.t1	AT4G24180	49.309	217	95	5	25	231	25	236	9.73e-66	207
MS.gene93762.t1	AT2G28790	44.538	238	123	6	14	245	39	273	5.69e-65	203
MS.gene93762.t1	AT2G28790	45.378	238	121	7	14	245	14	248	6.34e-65	202
MS.gene93762.t1	AT5G38280	43.568	241	129	6	14	247	11	251	1.80e-59	199
MS.gene93762.t1	AT5G38280	43.568	241	129	6	14	247	11	251	2.52e-59	199
MS.gene93762.t1	AT4G11650	40.239	251	123	7	1	249	1	226	1.25e-57	184
MS.gene93762.t1	AT4G36000	58.503	147	51	3	103	247	1	139	1.51e-54	174
MS.gene93762.t1	AT4G36000	58.503	147	51	3	103	247	1	139	1.51e-54	174
MS.gene93762.t1	AT4G18253	39.496	238	136	4	14	249	10	241	3.60e-52	170
MS.gene93762.t1	AT1G70250	38.326	227	126	8	29	246	146	367	5.62e-42	152
MS.gene93762.t1	AT4G18250	37.288	236	139	6	14	246	43	272	3.86e-39	144
MS.gene93762.t1	AT5G24620	52.800	125	53	3	132	250	15	139	1.07e-35	129
MS.gene93762.t1	AT2G24810	58.442	77	32	0	169	245	116	192	1.43e-24	97.1

Find 96 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAATGGTCAGGCCGTTTC+TGG	0.068116	4.2:+317032	MS.gene93762:CDS
CCGGCACCGGGTTGGATTGC+TGG	0.183097	4.2:-316941	None:intergenic
TGAATAAGCAGTGGGCTTGC+AGG	0.307584	4.2:-317428	None:intergenic
GACCTCACCTGCAACCCTAC+TGG	0.350758	4.2:-317310	None:intergenic
CGGTGCCGGAAAACCCATTC+TGG	0.376903	4.2:+316955	MS.gene93762:CDS
CTGACCATTGTGGTGGGACT+TGG	0.379600	4.2:-317021	None:intergenic
GAAGCCGCCGCGGGCCAGAA+TGG	0.391505	4.2:-316969	None:intergenic
ACAATGGTCAGGCCGTTTCT+GGG	0.391880	4.2:+317033	MS.gene93762:CDS
ATTGCTGGCCACACTGGGTG+GGG	0.395326	4.2:-316926	None:intergenic
TGGAGGGAATAAGCTTTGTT+TGG	0.398076	4.2:-317001	None:intergenic
TAAGCATAAGAGTAAGCCTT+TGG	0.402209	4.2:-317472	None:intergenic
GGTTGGATTGCTGGCCACAC+TGG	0.402359	4.2:-316932	None:intergenic
ACACTCGCTGAGTTCACATT+TGG	0.414011	4.2:+317160	MS.gene93762:CDS
AAGCCGCCGCGGGCCAGAAT+GGG	0.420312	4.2:-316968	None:intergenic
GATGTGCCCAGTAGGGTTGC+AGG	0.421685	4.2:+317303	MS.gene93762:CDS
CGCAGTCTCCGGTGGCGCAA+TGG	0.424632	4.2:-317093	None:intergenic
ATTACACCTTCCAAGGGATC+TGG	0.427743	4.2:+317241	MS.gene93762:CDS
CAGTGGGCTTGCAGGTCTGT+GGG	0.433412	4.2:-317420	None:intergenic
ATTGGTGCAAGTAGCAATAC+TGG	0.439699	4.2:-317506	None:intergenic
ACCTCACCTGCAACCCTACT+GGG	0.443228	4.2:-317309	None:intergenic
GGCCACGGCCATTGCGCCAC+CGG	0.444546	4.2:+317085	MS.gene93762:CDS
GGATTGCTGGCCACACTGGG+TGG	0.446029	4.2:-316928	None:intergenic
GGGAATAAGCTTTGTTTGGT+GGG	0.446624	4.2:-316997	None:intergenic
CTCCGGTGGCGCAATGGCCG+TGG	0.450871	4.2:-317087	None:intergenic
TTGGAAGGTGTAATGGAAAT+CGG	0.452808	4.2:-317232	None:intergenic
GTTGGATTGCTGGCCACACT+GGG	0.457650	4.2:-316931	None:intergenic
ACCATTGTGGTGGGACTTGG+AGG	0.458699	4.2:-317018	None:intergenic
GATTGCTGGCCACACTGGGT+GGG	0.464130	4.2:-316927	None:intergenic
GCAGTGGGCTTGCAGGTCTG+TGG	0.469723	4.2:-317421	None:intergenic
GATTTCCATTACACCTTCCA+AGG	0.470223	4.2:+317234	MS.gene93762:CDS
AGATCCCTTGGAAGGTGTAA+TGG	0.470965	4.2:-317239	None:intergenic
TACCTGGAGGGTATGCGATT+AGG	0.471659	4.2:-316724	None:intergenic
GGCTGCACCTTCGACGCATC+AGG	0.474004	4.2:+317064	MS.gene93762:CDS
GAAGAAGAGAGCGGTGAAGA+TGG	0.478141	4.2:-316693	None:intergenic
GAGAGATCCGCCGCAGTCTC+CGG	0.481610	4.2:-317104	None:intergenic
AAGCATAAGAGTAAGCCTTT+GGG	0.484229	4.2:-317471	None:intergenic
CAAGTCCCACCACAATGGTC+AGG	0.493666	4.2:+317022	MS.gene93762:CDS
AGGGAATAAGCTTTGTTTGG+TGG	0.495351	4.2:-316998	None:intergenic
GGCCGTTTCTGGGGCCGTCA+CGG	0.495582	4.2:+317043	MS.gene93762:CDS
AGCCGTGACGGCCCCAGAAA+CGG	0.498382	4.2:-317045	None:intergenic
TATTGCTGCACTGGTAACTT+TGG	0.498988	4.2:+317394	MS.gene93762:CDS
CCAGCAATCCAACCCGGTGC+CGG	0.501469	4.2:+316941	MS.gene93762:CDS
TCTCTCTTCTGCAACGGCAT+AGG	0.508531	4.2:+317121	MS.gene93762:CDS
TTCCTAATCGCATACCCTCC+AGG	0.509059	4.2:+316722	MS.gene93762:CDS
TCTCCTAGGTATTGCTGCAC+TGG	0.513261	4.2:+317385	MS.gene93762:CDS
GCCCAGTAGGGTTGCAGGTG+AGG	0.513868	4.2:+317308	MS.gene93762:CDS
TCTGGAAAATGTAGCTATGC+TGG	0.515580	4.2:+317259	MS.gene93762:CDS
TGGGAGCTTGAAGCCGCCGC+GGG	0.524917	4.2:-316978	None:intergenic
GTGAACTCAGCGAGTGTGGC+TGG	0.529115	4.2:-317154	None:intergenic
CTCTTCTGCAACGGCATAGG+CGG	0.529119	4.2:+317124	MS.gene93762:CDS
ACTCAGCGAGTGTGGCTGGT+GGG	0.531396	4.2:-317150	None:intergenic
AGTGATCATGTAACTAGCAT+TGG	0.535613	4.2:-317524	None:intergenic
CTCAGCGAGTGTGGCTGGTG+GGG	0.539267	4.2:-317149	None:intergenic
CTTAACTTGATGTGCCCAGT+AGG	0.539656	4.2:+317295	MS.gene93762:CDS
GTGCAAGTAGCAATACTGGT+AGG	0.548800	4.2:-317502	None:intergenic
AAGATTCTTGAATAAGCAGT+GGG	0.550542	4.2:-317436	None:intergenic
CGTCGAAGGTGCAGCCGTGA+CGG	0.550989	4.2:-317057	None:intergenic
GTAAGTTGTAACCGTCGACC+AGG	0.554402	4.2:-317210	None:intergenic
GTGCTGCTTTCAACTCTCCT+AGG	0.556468	4.2:+317371	MS.gene93762:CDS
AACTCAGCGAGTGTGGCTGG+TGG	0.563622	4.2:-317151	None:intergenic
AGATTTCTATGACGTCAGCC+TGG	0.567905	4.2:+317192	MS.gene93762:CDS
CAATGGTCAGGCCGTTTCTG+GGG	0.570580	4.2:+317034	MS.gene93762:CDS
CTTCAAAACTGCTTGCCCAA+AGG	0.572482	4.2:+317456	MS.gene93762:CDS
GGTGAAGATGGAAGCAGCCA+TGG	0.573489	4.2:-316681	None:intergenic
GAAGATTCTTGAATAAGCAG+TGG	0.575322	4.2:-317437	None:intergenic
CATTGCGCCACCGGAGACTG+CGG	0.577164	4.2:+317094	MS.gene93762:CDS
GTGTGGCCAGCAATCCAACC+CGG	0.580526	4.2:+316935	MS.gene93762:CDS
ATGATCAACGGCGGTGACGA+GGG	0.581144	4.2:-317552	None:intergenic
GCCGTGGCCTGATGCGTCGA+AGG	0.582247	4.2:-317071	None:intergenic
TTAACTTGATGTGCCCAGTA+GGG	0.582603	4.2:+317296	MS.gene93762:CDS
GTGGGAGCTTGAAGCCGCCG+CGG	0.590614	4.2:-316979	None:intergenic
ACCTTCGACGCATCAGGCCA+CGG	0.592438	4.2:+317070	MS.gene93762:CDS
TATGACGTCAGCCTGGTCGA+CGG	0.603237	4.2:+317199	MS.gene93762:CDS
GATGATCAACGGCGGTGACG+AGG	0.606071	4.2:-317553	None:intergenic
GGCGGATCTCTCTTCTGCAA+CGG	0.609526	4.2:+317115	MS.gene93762:CDS
TGCAAGTAGCAATACTGGTA+GGG	0.611972	4.2:-317501	None:intergenic
ATTTCCATTACACCTTCCAA+GGG	0.612133	4.2:+317235	MS.gene93762:CDS
AAACGGCCTGACCATTGTGG+TGG	0.613091	4.2:-317028	None:intergenic
TGCGCCACCGGAGACTGCGG+CGG	0.616529	4.2:+317097	MS.gene93762:CDS
TAATAAAATAAATACCTGGA+GGG	0.617393	4.2:-316736	None:intergenic
AGTGGGCTTGCAGGTCTGTG+GGG	0.617801	4.2:-317419	None:intergenic
TTACCAGTGCAGCAATACCT+AGG	0.621497	4.2:-317388	None:intergenic
AGATCCGCCGCAGTCTCCGG+TGG	0.621709	4.2:-317101	None:intergenic
CGTGACAAGAAGCAAGTGGT+GGG	0.622854	4.2:+317334	MS.gene93762:CDS
CAGAAACGGCCTGACCATTG+TGG	0.626293	4.2:-317031	None:intergenic
AACGGCCTGACCATTGTGGT+GGG	0.629227	4.2:-317027	None:intergenic
CCATTGTGGTGGGACTTGGA+GGG	0.634133	4.2:-317017	None:intergenic
ACGTGACAAGAAGCAAGTGG+TGG	0.639156	4.2:+317333	MS.gene93762:CDS
GGAAAACCCATTCTGGCCCG+CGG	0.640848	4.2:+316962	MS.gene93762:CDS
AAATGTGAACTCAGCGAGTG+TGG	0.640994	4.2:-317158	None:intergenic
CCCTCCAAGTCCCACCACAA+TGG	0.642784	4.2:+317017	MS.gene93762:CDS
AAACCCATTCTGGCCCGCGG+CGG	0.643152	4.2:+316965	MS.gene93762:CDS
GAAAGCAGCGAAGAAGAGAG+CGG	0.656778	4.2:-316702	None:intergenic
ACAAGTGTCCCCACCCAGTG+TGG	0.666782	4.2:+316918	MS.gene93762:CDS
GTCACGTGACAAGAAGCAAG+TGG	0.679782	4.2:+317330	MS.gene93762:CDS
TGAAGATAGATGATCAACGG+CGG	0.740375	4.2:-317561	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATAATAAAATAAATACC+TGG	-	chr4.2:316743-316762	None:intergenic	10.0%
!!	ATAATAAAATAAATACCTGG+AGG	-	chr4.2:316740-316759	None:intergenic	20.0%
!!	TAATAAAATAAATACCTGGA+GGG	-	chr4.2:316739-316758	None:intergenic	20.0%
!	AGAAACGACAAATTAAGTAA+CGG	-	chr4.2:316839-316858	None:intergenic	25.0%
!!!	ATTTTAACAAACAGTAGTGA+AGG	+	chr4.2:316865-316884	MS.gene93762:intron	25.0%
	AAGATTCTTGAATAAGCAGT+GGG	-	chr4.2:317439-317458	None:intergenic	30.0%
	AAGCATAAGAGTAAGCCTTT+GGG	-	chr4.2:317474-317493	None:intergenic	35.0%
	AGTGATCATGTAACTAGCAT+TGG	-	chr4.2:317527-317546	None:intergenic	35.0%
	ATTTCCATTACACCTTCCAA+GGG	+	chr4.2:317235-317254	MS.gene93762:CDS	35.0%
	GAAGATTCTTGAATAAGCAG+TGG	-	chr4.2:317440-317459	None:intergenic	35.0%
	TAAGCATAAGAGTAAGCCTT+TGG	-	chr4.2:317475-317494	None:intergenic	35.0%
!	TTGGAAGGTGTAATGGAAAT+CGG	-	chr4.2:317235-317254	None:intergenic	35.0%
	ATTGGTGCAAGTAGCAATAC+TGG	-	chr4.2:317509-317528	None:intergenic	40.0%
	GATTTCCATTACACCTTCCA+AGG	+	chr4.2:317234-317253	MS.gene93762:CDS	40.0%
	TCTGGAAAATGTAGCTATGC+TGG	+	chr4.2:317259-317278	MS.gene93762:CDS	40.0%
	TGCAAGTAGCAATACTGGTA+GGG	-	chr4.2:317504-317523	None:intergenic	40.0%
!	TATTGCTGCACTGGTAACTT+TGG	+	chr4.2:317394-317413	MS.gene93762:CDS	40.0%
!	TTAACTTGATGTGCCCAGTA+GGG	+	chr4.2:317296-317315	MS.gene93762:CDS	40.0%
!!	AGGGAATAAGCTTTGTTTGG+TGG	-	chr4.2:317001-317020	None:intergenic	40.0%
!!	GGGAATAAGCTTTGTTTGGT+GGG	-	chr4.2:317000-317019	None:intergenic	40.0%
!!	TGGAGGGAATAAGCTTTGTT+TGG	-	chr4.2:317004-317023	None:intergenic	40.0%
	AAATGTGAACTCAGCGAGTG+TGG	-	chr4.2:317161-317180	None:intergenic	45.0%
	ACACTCGCTGAGTTCACATT+TGG	+	chr4.2:317160-317179	MS.gene93762:CDS	45.0%
	AGATTTCTATGACGTCAGCC+TGG	+	chr4.2:317192-317211	MS.gene93762:CDS	45.0%
	ATTACACCTTCCAAGGGATC+TGG	+	chr4.2:317241-317260	MS.gene93762:CDS	45.0%
	CTTCAAAACTGCTTGCCCAA+AGG	+	chr4.2:317456-317475	MS.gene93762:CDS	45.0%
	GTGCAAGTAGCAATACTGGT+AGG	-	chr4.2:317505-317524	None:intergenic	45.0%
	TCACACACACACACAGAAAG+CGG	+	chr4.2:316782-316801	MS.gene93762:intron	45.0%
	TGTGTGTGAGAGAGAAGAGA+AGG	-	chr4.2:316771-316790	None:intergenic	45.0%
	TTACCAGTGCAGCAATACCT+AGG	-	chr4.2:317391-317410	None:intergenic	45.0%
!	AGATCCCTTGGAAGGTGTAA+TGG	-	chr4.2:317242-317261	None:intergenic	45.0%
!	CATTTTCCAGATCCCTTGGA+AGG	-	chr4.2:317250-317269	None:intergenic	45.0%
!	CTTAACTTGATGTGCCCAGT+AGG	+	chr4.2:317295-317314	MS.gene93762:CDS	45.0%
!	GCTACATTTTCCAGATCCCT+TGG	-	chr4.2:317254-317273	None:intergenic	45.0%
	ACAATGGTCAGGCCGTTTCT+GGG	+	chr4.2:317033-317052	MS.gene93762:CDS	50.0%
	ACGTGACAAGAAGCAAGTGG+TGG	+	chr4.2:317333-317352	MS.gene93762:CDS	50.0%
	CGTGACAAGAAGCAAGTGGT+GGG	+	chr4.2:317334-317353	MS.gene93762:CDS	50.0%
	GAAAGCAGCGAAGAAGAGAG+CGG	-	chr4.2:316705-316724	None:intergenic	50.0%
	GAAGAAGAGAGCGGTGAAGA+TGG	-	chr4.2:316696-316715	None:intergenic	50.0%
	GTAAGTTGTAACCGTCGACC+AGG	-	chr4.2:317213-317232	None:intergenic	50.0%
	GTCACGTGACAAGAAGCAAG+TGG	+	chr4.2:317330-317349	MS.gene93762:CDS	50.0%
	GTGTGAGAGAGAAGAGAAGG+AGG	-	chr4.2:316768-316787	None:intergenic	50.0%
	TCTCTCTTCTGCAACGGCAT+AGG	+	chr4.2:317121-317140	MS.gene93762:CDS	50.0%
	TTCCTAATCGCATACCCTCC+AGG	+	chr4.2:316722-316741	MS.gene93762:CDS	50.0%
!	TACCTGGAGGGTATGCGATT+AGG	-	chr4.2:316727-316746	None:intergenic	50.0%
!	TCTCCTAGGTATTGCTGCAC+TGG	+	chr4.2:317385-317404	MS.gene93762:CDS	50.0%
!	TGAATAAGCAGTGGGCTTGC+AGG	-	chr4.2:317431-317450	None:intergenic	50.0%
!!	GTGCTGCTTTCAACTCTCCT+AGG	+	chr4.2:317371-317390	MS.gene93762:CDS	50.0%
	AAACGGCCTGACCATTGTGG+TGG	-	chr4.2:317031-317050	None:intergenic	55.0%
	AACGGCCTGACCATTGTGGT+GGG	-	chr4.2:317030-317049	None:intergenic	55.0%
	ACCTCACCTGCAACCCTACT+GGG	-	chr4.2:317312-317331	None:intergenic	55.0%
	CAAGTCCCACCACAATGGTC+AGG	+	chr4.2:317022-317041	MS.gene93762:CDS	55.0%
	CAATGGTCAGGCCGTTTCTG+GGG	+	chr4.2:317034-317053	MS.gene93762:CDS	55.0%
	CACAATGGTCAGGCCGTTTC+TGG	+	chr4.2:317032-317051	MS.gene93762:CDS	55.0%
	CAGAAACGGCCTGACCATTG+TGG	-	chr4.2:317034-317053	None:intergenic	55.0%
	CTCTTCTGCAACGGCATAGG+CGG	+	chr4.2:317124-317143	MS.gene93762:CDS	55.0%
	GGCGGATCTCTCTTCTGCAA+CGG	+	chr4.2:317115-317134	MS.gene93762:CDS	55.0%
	GTTGGATTGCTGGCCACACT+GGG	-	chr4.2:316934-316953	None:intergenic	55.0%
	TATGACGTCAGCCTGGTCGA+CGG	+	chr4.2:317199-317218	MS.gene93762:CDS	55.0%
!	ACCATTGTGGTGGGACTTGG+AGG	-	chr4.2:317021-317040	None:intergenic	55.0%
!	CCATTGTGGTGGGACTTGGA+GGG	-	chr4.2:317020-317039	None:intergenic	55.0%
!	CTGACCATTGTGGTGGGACT+TGG	-	chr4.2:317024-317043	None:intergenic	55.0%
!!	AGAATGGGTTTTCCGGCACC+GGG	-	chr4.2:316956-316975	None:intergenic	55.0%
!!	CAGAATGGGTTTTCCGGCAC+CGG	-	chr4.2:316957-316976	None:intergenic	55.0%
	AACTCAGCGAGTGTGGCTGG+TGG	-	chr4.2:317154-317173	None:intergenic	60.0%
	ACAAGTGTCCCCACCCAGTG+TGG	+	chr4.2:316918-316937	MS.gene93762:CDS	60.0%
	ACCTTCGACGCATCAGGCCA+CGG	+	chr4.2:317070-317089	MS.gene93762:CDS	60.0%
	ACTCAGCGAGTGTGGCTGGT+GGG	-	chr4.2:317153-317172	None:intergenic	60.0%
	ATTGCTGGCCACACTGGGTG+GGG	-	chr4.2:316929-316948	None:intergenic	60.0%
	CCCTCCAAGTCCCACCACAA+TGG	+	chr4.2:317017-317036	MS.gene93762:CDS	60.0%
	GACCTCACCTGCAACCCTAC+TGG	-	chr4.2:317313-317332	None:intergenic	60.0%
	GATGTGCCCAGTAGGGTTGC+AGG	+	chr4.2:317303-317322	MS.gene93762:CDS	60.0%
	GATTGCTGGCCACACTGGGT+GGG	-	chr4.2:316930-316949	None:intergenic	60.0%
	GGAAAACCCATTCTGGCCCG+CGG	+	chr4.2:316962-316981	MS.gene93762:CDS	60.0%
	GGTTGGATTGCTGGCCACAC+TGG	-	chr4.2:316935-316954	None:intergenic	60.0%
	GTGAACTCAGCGAGTGTGGC+TGG	-	chr4.2:317157-317176	None:intergenic	60.0%
	GTGTGGCCAGCAATCCAACC+CGG	+	chr4.2:316935-316954	MS.gene93762:CDS	60.0%
!	AGTGGGCTTGCAGGTCTGTG+GGG	-	chr4.2:317422-317441	None:intergenic	60.0%
!	CAGTGGGCTTGCAGGTCTGT+GGG	-	chr4.2:317423-317442	None:intergenic	60.0%
!	CGGTGCCGGAAAACCCATTC+TGG	+	chr4.2:316955-316974	MS.gene93762:CDS	60.0%
!!	GCGGGCCAGAATGGGTTTTC+CGG	-	chr4.2:316963-316982	None:intergenic	60.0%
	AAACCCATTCTGGCCCGCGG+CGG	+	chr4.2:316965-316984	MS.gene93762:CDS	65.0%
	AGCCGTGACGGCCCCAGAAA+CGG	-	chr4.2:317048-317067	None:intergenic	65.0%
	CATTGCGCCACCGGAGACTG+CGG	+	chr4.2:317094-317113	MS.gene93762:CDS	65.0%
	CCAGCAATCCAACCCGGTGC+CGG	+	chr4.2:316941-316960	MS.gene93762:CDS	65.0%
	CTCAGCGAGTGTGGCTGGTG+GGG	-	chr4.2:317152-317171	None:intergenic	65.0%
	GAGAGATCCGCCGCAGTCTC+CGG	-	chr4.2:317107-317126	None:intergenic	65.0%
	GCCCAGTAGGGTTGCAGGTG+AGG	+	chr4.2:317308-317327	MS.gene93762:CDS	65.0%
	GGATTGCTGGCCACACTGGG+TGG	-	chr4.2:316931-316950	None:intergenic	65.0%
	GGCTGCACCTTCGACGCATC+AGG	+	chr4.2:317064-317083	MS.gene93762:CDS	65.0%
!	CGTCGAAGGTGCAGCCGTGA+CGG	-	chr4.2:317060-317079	None:intergenic	65.0%
!	GCAGTGGGCTTGCAGGTCTG+TGG	-	chr4.2:317424-317443	None:intergenic	65.0%
!!	TGGGTTTTCCGGCACCGGGT+TGG	-	chr4.2:316952-316971	None:intergenic	65.0%
	AAGCCGCCGCGGGCCAGAAT+GGG	-	chr4.2:316971-316990	None:intergenic	70.0%
	AGATCCGCCGCAGTCTCCGG+TGG	-	chr4.2:317104-317123	None:intergenic	70.0%
	CGCAGTCTCCGGTGGCGCAA+TGG	-	chr4.2:317096-317115	None:intergenic	70.0%
	GCCGTGGCCTGATGCGTCGA+AGG	-	chr4.2:317074-317093	None:intergenic	70.0%
	GTGGGAGCTTGAAGCCGCCG+CGG	-	chr4.2:316982-317001	None:intergenic	70.0%
	TGGGAGCTTGAAGCCGCCGC+GGG	-	chr4.2:316981-317000	None:intergenic	70.0%
!	CCGGCACCGGGTTGGATTGC+TGG	-	chr4.2:316944-316963	None:intergenic	70.0%
!!	GGCCGTTTCTGGGGCCGTCA+CGG	+	chr4.2:317043-317062	MS.gene93762:CDS	70.0%
	CTCCGGTGGCGCAATGGCCG+TGG	-	chr4.2:317090-317109	None:intergenic	75.0%
	GAAGCCGCCGCGGGCCAGAA+TGG	-	chr4.2:316972-316991	None:intergenic	75.0%
	GGCCACGGCCATTGCGCCAC+CGG	+	chr4.2:317085-317104	MS.gene93762:CDS	75.0%
	TGCGCCACCGGAGACTGCGG+CGG	+	chr4.2:317097-317116	MS.gene93762:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	316683	317570	316683	ID=MS.gene93762
chr4.2	mRNA	316683	317570	316683	ID=MS.gene93762.t1;Parent=MS.gene93762
chr4.2	exon	316683	316743	316683	ID=MS.gene93762.t1.exon1;Parent=MS.gene93762.t1
chr4.2	CDS	316683	316743	316683	ID=cds.MS.gene93762.t1;Parent=MS.gene93762.t1
chr4.2	exon	316879	317570	316879	ID=MS.gene93762.t1.exon2;Parent=MS.gene93762.t1
chr4.2	CDS	316879	317570	316879	ID=cds.MS.gene93762.t1;Parent=MS.gene93762.t1

Gene Sequence

>MS.gene93762

ATGGCTGCTTCCATCTTCACCGCTCTCTTCTTCGCTGCTTTCCTAATCGCATACCCTCCAGGTATTTATTTTATTATTATTTCCTCCTTCTCTTCTCTCTCACACACACACACAGAAAGCGGTTTGTGAGAGATATATAGTACATTTATTACACCGTTACTTAATTTGTCGTTTCTTGTTTGATTTTAACAAACAGTAGTGAAGGTATCAGCAGCAGCATCAGTAGTATTTTACAACAAGTGTCCCCACCCAGTGTGGCCAGCAATCCAACCCGGTGCCGGAAAACCCATTCTGGCCCGCGGCGGCTTCAAGCTCCCACCAAACAAAGCTTATTCCCTCCAAGTCCCACCACAATGGTCAGGCCGTTTCTGGGGCCGTCACGGCTGCACCTTCGACGCATCAGGCCACGGCCATTGCGCCACCGGAGACTGCGGCGGATCTCTCTTCTGCAACGGCATAGGCGGAACCCCACCAGCCACACTCGCTGAGTTCACATTTGGAAAAGATCTAGATTTCTATGACGTCAGCCTGGTCGACGGTTACAACTTACCGATTTCCATTACACCTTCCAAGGGATCTGGAAAATGTAGCTATGCTGGCTGTGTGAGTGATCTTAACTTGATGTGCCCAGTAGGGTTGCAGGTGAGGTCACGTGACAAGAAGCAAGTGGTGGGTTGTAGAAGTGCGTGTGCTGCTTTCAACTCTCCTAGGTATTGCTGCACTGGTAACTTTGGTACCCCACAGACCTGCAAGCCCACTGCTTATTCAAGAATCTTCAAAACTGCTTGCCCAAAGGCTTACTCTTATGCTTATGATGACCCTACCAGTATTGCTACTTGCACCAATGCTAGTTACATGATCACTTTCTGCCCTCGTCACCGCCGTTGA

Protein sequence

>MS.gene93762.t1

MAASIFTALFFAAFLIAYPPVVKVSAAASVVFYNKCPHPVWPAIQPGAGKPILARGGFKLPPNKAYSLQVPPQWSGRFWGRHGCTFDASGHGHCATGDCGGSLFCNGIGGTPPATLAEFTFGKDLDFYDVSLVDGYNLPISITPSKGSGKCSYAGCVSDLNLMCPVGLQVRSRDKKQVVGCRSACAAFNSPRYCCTGNFGTPQTCKPTAYSRIFKTACPKAYSYAYDDPTSIATCTNASYMITFCPRHRR