AlfalfaGEDB | MS.gene27345

M. sativa cultivar XinJiangDaYe / MS.gene27345

KEGG: -
GO: -
PFAM: PF00314.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27345.t1	XP_013466052.1	95.6	249	11	0	1	249	1	249	7.90E-136	493

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27345.t1	Q5DWG1	66.2	234	72	3	15	246	11	239	1.7e-86	320.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27345.t1	A0A072VEQ3	95.6	249	11	0	1	249	1	249	5.7e-136	493.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene022225	MS.gene27345	PPI
MS.gene00249	MS.gene27345	PPI
MS.gene040126	MS.gene27345	PPI
MS.gene056184	MS.gene27345	PPI
MS.gene004055	MS.gene27345	PPI
MS.gene044112	MS.gene27345	PPI
MS.gene057702	MS.gene27345	PPI
MS.gene41190	MS.gene27345	PPI
MS.gene27345	MS.gene69123	PPI
MS.gene92397	MS.gene27345	PPI
MS.gene003526	MS.gene27345	PPI
MS.gene27345	MS.gene010631	PPI
MS.gene92395	MS.gene27345	PPI
MS.gene64778	MS.gene27345	PPI
MS.gene016652	MS.gene27345	PPI
MS.gene59476	MS.gene27345	PPI
MS.gene27345	MS.gene046990	PPI
MS.gene27345	MS.gene022252	PPI
MS.gene61094	MS.gene27345	PPI
MS.gene68072	MS.gene27345	PPI
MS.gene27345	MS.gene92393	PPI
MS.gene93762	MS.gene27345	PPI
MS.gene27345	MS.gene021099	PPI
MS.gene062716	MS.gene27345	PPI
MS.gene20542	MS.gene27345	PPI
MS.gene050380	MS.gene27345	PPI
MS.gene24315	MS.gene27345	PPI
MS.gene27345	MS.gene25909	PPI
MS.gene45764	MS.gene27345	PPI
MS.gene038936	MS.gene27345	PPI
MS.gene42360	MS.gene27345	PPI
MS.gene38404	MS.gene27345	PPI
MS.gene27345	MS.gene015555	PPI
MS.gene27345	MS.gene014545	PPI
MS.gene90983	MS.gene27345	PPI
MS.gene56731	MS.gene27345	PPI
MS.gene072805	MS.gene27345	PPI
MS.gene38414	MS.gene27345	PPI
MS.gene38392	MS.gene27345	PPI
MS.gene69123	MS.gene27345	PPI
MS.gene25879	MS.gene27345	PPI
MS.gene019285	MS.gene27345	PPI
MS.gene074397	MS.gene27345	PPI
MS.gene38399	MS.gene27345	PPI
MS.gene70582	MS.gene27345	PPI
MS.gene94553	MS.gene27345	PPI
MS.gene52073	MS.gene27345	PPI
MS.gene38408	MS.gene27345	PPI
MS.gene014545	MS.gene27345	PPI
MS.gene056186	MS.gene27345	PPI
MS.gene42714	MS.gene27345	PPI
MS.gene27345	MS.gene26931	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27345.t1	MTR_1g021945	95.582	249	11	0	1	249	1	249	1.01e-164	455
MS.gene27345.t1	MTR_3g111620	74.104	251	62	3	1	249	1	250	1.34e-129	367
MS.gene27345.t1	MTR_8g036215	52.033	246	107	4	1	244	1	237	2.23e-86	256
MS.gene27345.t1	MTR_5g022350	55.372	242	96	6	7	245	11	243	1.12e-83	253
MS.gene27345.t1	MTR_6g079580	52.479	242	103	5	11	245	4	240	1.56e-83	249
MS.gene27345.t1	MTR_4g073720	54.043	235	100	4	12	244	11	239	4.70e-83	252
MS.gene27345.t1	MTR_8g056820	51.383	253	115	5	3	249	1	251	2.30e-82	247
MS.gene27345.t1	MTR_8g107140	52.869	244	105	4	9	246	7	246	2.38e-81	244
MS.gene27345.t1	MTR_8g037890	52.459	244	106	5	9	248	13	250	1.00e-80	245
MS.gene27345.t1	MTR_8g075510	53.689	244	101	6	8	248	13	247	5.50e-80	241
MS.gene27345.t1	MTR_1g025420	55.230	239	95	7	16	244	9	245	1.26e-79	243
MS.gene27345.t1	MTR_1g025420	54.583	240	100	7	11	244	9	245	1.36e-79	240
MS.gene27345.t1	MTR_3g114030	55.702	228	93	6	25	246	6	231	2.68e-79	239
MS.gene27345.t1	MTR_1g062590	55.983	234	96	6	16	245	12	242	3.22e-79	238
MS.gene27345.t1	MTR_8g075550	54.812	239	96	5	19	248	22	257	1.04e-78	239
MS.gene27345.t1	MTR_8g075510	53.750	240	99	6	8	244	13	243	3.64e-78	238
MS.gene27345.t1	MTR_4g073730	52.459	244	106	6	10	245	7	248	2.34e-76	233
MS.gene27345.t1	MTR_4g073730	52.459	244	106	6	10	245	7	248	5.65e-76	233
MS.gene27345.t1	MTR_2g067980	51.220	246	111	6	7	245	4	247	3.63e-75	246
MS.gene27345.t1	MTR_5g022310	49.402	251	114	6	10	249	7	255	4.01e-75	229
MS.gene27345.t1	MTR_1g062660	51.429	245	111	7	6	246	3	243	4.92e-75	228
MS.gene27345.t1	MTR_5g022310	49.402	251	114	6	10	249	7	255	8.67e-75	229
MS.gene27345.t1	MTR_1g062340	51.852	243	109	7	8	246	5	243	1.60e-74	227
MS.gene27345.t1	MTR_1g062350	52.340	235	105	6	16	246	12	243	1.86e-74	226
MS.gene27345.t1	MTR_2g069660	53.333	240	105	6	10	245	7	243	2.69e-74	226
MS.gene27345.t1	MTR_5g059200	52.018	223	99	2	24	246	27	241	2.78e-74	228
MS.gene27345.t1	MTR_7g102380	52.915	223	98	6	28	246	23	242	5.01e-74	225
MS.gene27345.t1	MTR_1g062370	51.489	235	107	6	16	246	12	243	3.12e-73	223
MS.gene27345.t1	MTR_2g068030	50.226	221	104	5	28	244	25	243	4.83e-73	223
MS.gene27345.t1	MTR_1g062630	52.766	235	104	6	16	246	12	243	7.89e-73	223
MS.gene27345.t1	MTR_7g062610	48.000	225	115	2	22	245	19	242	5.13e-69	213
MS.gene27345.t1	MTR_3g068015	45.344	247	124	4	9	245	3	248	1.17e-68	212
MS.gene27345.t1	MTR_6g009480	45.122	246	126	7	9	247	11	254	1.08e-66	207
MS.gene27345.t1	MTR_2g068655	46.696	227	115	5	23	245	28	252	8.63e-66	207
MS.gene27345.t1	MTR_1g062690	45.957	235	98	5	16	247	12	220	2.06e-60	190
MS.gene27345.t1	MTR_4g063630	42.083	240	123	8	15	244	18	251	5.69e-56	179
MS.gene27345.t1	MTR_5g010635	42.795	229	107	8	23	248	21	228	2.61e-52	170
MS.gene27345.t1	MTR_5g010640	39.516	248	123	8	8	245	6	236	8.49e-48	158
MS.gene27345.t1	MTR_8g096900	39.241	237	121	7	11	245	33	248	7.60e-47	156
MS.gene27345.t1	MTR_2g067990	42.534	221	97	9	28	245	29	222	2.21e-44	159
MS.gene27345.t1	MTR_5g022350	53.595	153	62	5	7	156	76	222	4.69e-43	145
MS.gene27345.t1	MTR_8g089020	50.382	131	60	1	107	237	31	156	2.06e-42	142
MS.gene27345.t1	MTR_2g067970	43.386	189	96	7	68	249	2	186	1.26e-38	143
MS.gene27345.t1	MTR_5g023850	35.081	248	115	9	10	249	11	220	6.53e-38	132
MS.gene27345.t1	MTR_2g063150	31.381	239	151	7	9	238	11	245	5.10e-32	118
MS.gene27345.t1	MTR_7g076360	35.484	217	74	5	23	237	5	157	3.36e-30	111
MS.gene27345.t1	MTR_1g062390	32.377	244	142	7	11	245	9	238	5.42e-30	112
MS.gene27345.t1	MTR_8g096910	45.113	133	63	3	10	141	6	129	1.05e-29	110
MS.gene27345.t1	MTR_8g096920	32.143	224	85	6	23	245	16	173	1.27e-26	102
MS.gene27345.t1	MTR_3g081550	43.750	96	49	3	109	201	16	109	5.32e-14	67.0
MS.gene27345.t1	MTR_1g062640	34.839	155	76	7	21	166	8	146	9.22e-13	64.7
MS.gene27345.t1	MTR_5g068670	47.561	82	38	3	63	143	12	89	7.65e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27345.t1	AT1G75030	67.917	240	73	3	11	248	9	246	6.74e-115	329
MS.gene27345.t1	AT1G75050	67.647	238	71	4	11	245	9	243	4.09e-111	320
MS.gene27345.t1	AT1G75040	63.830	235	81	3	11	245	9	239	3.44e-105	304
MS.gene27345.t1	AT1G19320	60.669	239	88	4	11	244	9	246	1.49e-95	280
MS.gene27345.t1	AT4G38660	57.676	241	94	4	11	249	15	249	6.99e-90	269
MS.gene27345.t1	AT4G38660	58.723	235	87	5	17	249	1	227	1.08e-87	263
MS.gene27345.t1	AT1G73620	54.626	227	96	3	23	246	39	261	2.87e-83	249
MS.gene27345.t1	AT4G24180	55.510	245	97	7	9	246	18	257	7.33e-82	246
MS.gene27345.t1	AT4G24180	55.510	245	97	7	9	246	18	257	1.26e-81	247
MS.gene27345.t1	AT1G77700	51.867	241	105	4	6	244	40	271	4.04e-81	246
MS.gene27345.t1	AT1G77700	51.867	241	105	4	6	244	40	271	4.11e-81	246
MS.gene27345.t1	AT4G24180	53.968	252	105	7	2	246	5	252	8.53e-81	243
MS.gene27345.t1	AT4G24180	53.968	252	105	7	2	246	5	252	2.22e-80	244
MS.gene27345.t1	AT1G75800	53.755	253	104	8	1	245	1	248	4.14e-80	244
MS.gene27345.t1	AT1G18250	53.744	227	98	3	23	246	18	240	7.42e-80	240
MS.gene27345.t1	AT1G77700	53.846	221	94	2	24	244	87	299	1.16e-79	244
MS.gene27345.t1	AT1G18250	52.863	227	100	3	23	246	19	241	3.53e-79	239
MS.gene27345.t1	AT5G24620	53.814	236	100	4	18	245	17	251	1.15e-78	243
MS.gene27345.t1	AT5G24620	53.814	236	100	4	18	245	72	306	2.24e-78	244
MS.gene27345.t1	AT5G24620	53.814	236	100	4	18	245	93	327	3.91e-78	244
MS.gene27345.t1	AT1G20030	56.388	227	89	6	28	246	5	229	6.97e-78	237
MS.gene27345.t1	AT1G20030	56.388	227	89	6	28	246	22	246	8.39e-78	238
MS.gene27345.t1	AT2G17860	55.645	248	97	8	10	248	9	252	1.94e-77	234
MS.gene27345.t1	AT4G36010	54.506	233	94	6	24	246	21	251	7.14e-75	229
MS.gene27345.t1	AT4G36010	54.506	233	94	6	24	246	21	251	7.14e-75	229
MS.gene27345.t1	AT4G24180	54.585	229	92	7	9	230	18	241	8.65e-73	225
MS.gene27345.t1	AT4G24180	52.966	236	100	7	2	230	5	236	1.11e-71	222
MS.gene27345.t1	AT5G40020	45.082	244	125	5	12	247	8	250	3.17e-69	214
MS.gene27345.t1	AT4G38670	49.796	245	112	8	10	245	5	247	3.84e-69	214
MS.gene27345.t1	AT4G38670	49.796	245	112	8	10	245	5	247	3.84e-69	214
MS.gene27345.t1	AT4G38670	52.444	225	98	7	28	245	9	231	1.16e-67	210
MS.gene27345.t1	AT5G02140	45.833	240	127	3	8	245	3	241	1.52e-65	204
MS.gene27345.t1	AT5G02140	45.679	243	129	3	8	248	3	244	3.26e-65	205
MS.gene27345.t1	AT5G38280	45.565	248	123	8	10	249	11	254	3.27e-60	201
MS.gene27345.t1	AT5G38280	45.565	248	123	8	10	249	11	254	3.33e-60	201
MS.gene27345.t1	AT2G28790	43.277	238	125	6	16	244	12	248	1.43e-56	181
MS.gene27345.t1	AT2G28790	43.750	240	121	7	16	244	37	273	2.04e-56	181
MS.gene27345.t1	AT4G11650	43.913	230	108	6	17	245	14	223	2.25e-56	181
MS.gene27345.t1	AT4G18253	41.463	246	132	5	7	249	6	242	4.85e-55	177
MS.gene27345.t1	AT4G36000	56.000	150	57	4	101	249	1	142	9.57e-49	160
MS.gene27345.t1	AT4G36000	56.000	150	57	4	101	249	1	142	9.57e-49	160
MS.gene27345.t1	AT1G70250	40.948	232	120	7	28	249	147	371	2.78e-48	170
MS.gene27345.t1	AT4G18250	40.249	241	134	6	10	247	41	274	1.04e-42	154
MS.gene27345.t1	AT5G24620	54.167	120	48	2	133	245	15	134	8.70e-35	126
MS.gene27345.t1	AT2G24810	63.636	77	28	0	168	244	116	192	2.78e-26	101

Find 96 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGGTTGGTCAGGACGCTTC+TGG	0.127184	1.2:-2764126	MS.gene27345:CDS
ATTTGGCCGGGAACTCTTTC+CGG	0.265847	1.2:-2764223	MS.gene27345:CDS
CGGGAACTCTTTCCGGCAAC+GGG	0.270690	1.2:-2764216	MS.gene27345:CDS
TTCAACATGTTCCTCTTATT+AGG	0.273557	1.2:-2764388	MS.gene27345:intron
TCAAGCACTTGCACGTGTTC+TGG	0.293907	1.2:-2763650	MS.gene27345:CDS
CATTGGGGCAAAACGTGATA+TGG	0.343492	1.2:+2763618	None:intergenic
GGTCAGGACGCTTCTGGGCT+CGG	0.343568	1.2:-2764120	MS.gene27345:CDS
GAAGTCCTTGCCACCATTAC+CGG	0.358634	1.2:+2763973	None:intergenic
ACATTGGCAGAATTCACTAT+TGG	0.360199	1.2:-2764001	MS.gene27345:CDS
GGCAACGGGGCTGCAATTCT+CGG	0.375846	1.2:-2764202	MS.gene27345:CDS
ACCAATGCAGTTACACCATT+TGG	0.377375	1.2:-2764240	MS.gene27345:CDS
GTGAATTCTGCCAATGTTAC+CGG	0.381859	1.2:+2764007	None:intergenic
GAGAATTGCAGCCCCGTTGC+CGG	0.382376	1.2:+2764204	None:intergenic
GGGTTGGTCAGGACGCTTCT+GGG	0.383926	1.2:-2764125	MS.gene27345:CDS
CCGGGAACTCTTTCCGGCAA+CGG	0.394394	1.2:-2764217	MS.gene27345:CDS
GTGGATGTCCGGCAGAGCTT+AGG	0.403966	1.2:-2763847	MS.gene27345:CDS
CCGTTGCCGGAAAGAGTTCC+CGG	0.409855	1.2:+2764217	None:intergenic
GGGTGTGTTGCGGACTTGAA+TGG	0.414444	1.2:-2763869	MS.gene27345:CDS
GAACATGTTGAAGAGAAGAA+AGG	0.426997	1.2:+2764399	None:intergenic
GGCGTGTAAGAGCGCGTGTT+TGG	0.435818	1.2:-2763801	MS.gene27345:CDS
GGACGCTTCTGGGCTCGGAC+TGG	0.446805	1.2:-2764115	MS.gene27345:CDS
ACTGCACCATCGGGTTGGTC+AGG	0.452166	1.2:-2764136	MS.gene27345:CDS
TATGATCATGATGATCCATT+GGG	0.460244	1.2:+2763602	None:intergenic
GCAAGCTTTCTTAAATATCT+CGG	0.462492	1.2:+2763706	None:intergenic
GCAAAACGTGATATGGTAAT+CGG	0.467360	1.2:+2763625	None:intergenic
TGGATGTCCGGCAGAGCTTA+GGG	0.471726	1.2:-2763846	MS.gene27345:CDS
CGCGTGTTTGGCGTTCAATA+CGG	0.474969	1.2:-2763789	MS.gene27345:CDS
ACAATGTAGGCATGGGTGTA+CGG	0.477021	1.2:-2763925	MS.gene27345:CDS
ACCGGTGATTGTCAATATGC+GGG	0.477080	1.2:-2763890	MS.gene27345:CDS
AATGTTGTTTACCTAATAAG+AGG	0.483808	1.2:+2764377	None:intergenic
TAGGGTGAACAGTGCAGGTT+CGG	0.484236	1.2:-2763828	MS.gene27345:CDS
CAATGTAGGCATGGGTGTAC+GGG	0.485572	1.2:-2763924	MS.gene27345:CDS
TGTGTCACAGGAGATTGTTC+CGG	0.487338	1.2:-2764061	MS.gene27345:CDS
CTATGATCATGATGATCCAT+TGG	0.489037	1.2:+2763601	None:intergenic
AGTTAGTTTGACTGCACCAT+CGG	0.493248	1.2:-2764146	MS.gene27345:CDS
GGAAAGAGTTCCCGGCCAAA+TGG	0.496406	1.2:+2764225	None:intergenic
ACTATTGGATCAGCCGGTAA+TGG	0.496770	1.2:-2763986	MS.gene27345:CDS
TTGTGGCGTGGCATGTTGGC+CGG	0.499950	1.2:+2763751	None:intergenic
GAGCTTAGGGTGAACAGTGC+AGG	0.505667	1.2:-2763833	MS.gene27345:CDS
CTGTTCACCCTAAGCTCTGC+CGG	0.506624	1.2:+2763839	None:intergenic
ATGCAGTTACACCATTTGGC+CGG	0.506869	1.2:-2764236	MS.gene27345:CDS
CGGACTGGTTGCGAATTTGA+CGG	0.511038	1.2:-2764100	MS.gene27345:CDS
GTTAGTTTGACTGCACCATC+GGG	0.514040	1.2:-2764145	MS.gene27345:CDS
TAAGCATAACTGTAAGCAGT+TGG	0.515449	1.2:+2763683	None:intergenic
TGCAGTTACACCATTTGGCC+GGG	0.515490	1.2:-2764235	MS.gene27345:CDS
GAACAGTGCAGGTTCGGTGG+TGG	0.517897	1.2:-2763822	MS.gene27345:CDS
GGCAATGGTAAATGTGTCAC+AGG	0.521889	1.2:-2764073	MS.gene27345:CDS
AGTCAAACTAACTGATGTAC+CGG	0.522716	1.2:+2764156	None:intergenic
AAGCATAACTGTAAGCAGTT+GGG	0.532279	1.2:+2763684	None:intergenic
CGACGGTTACAATGTAGGCA+TGG	0.532942	1.2:-2763933	MS.gene27345:CDS
GGACTTCTACGATGTGAGCT+TGG	0.532975	1.2:-2763957	MS.gene27345:CDS
GAATTCACTATTGGATCAGC+CGG	0.534190	1.2:-2763992	MS.gene27345:CDS
GGACTTGAATGGTGGATGTC+CGG	0.536818	1.2:-2763858	MS.gene27345:CDS
GGTTGCGAATTTGACGGTGC+AGG	0.549983	1.2:-2764094	MS.gene27345:CDS
TCCGATACAGTTTAGTCCAC+CGG	0.550929	1.2:+2764042	None:intergenic
GTAACTGCATTGGTTGTGTA+TGG	0.552667	1.2:+2764249	None:intergenic
GAATTTGACGGTGCAGGCAA+TGG	0.556474	1.2:-2764088	MS.gene27345:CDS
TTGGTCGACGGTTACAATGT+AGG	0.559348	1.2:-2763938	MS.gene27345:CDS
CCCCGCATATTGACAATCAC+CGG	0.568073	1.2:+2763889	None:intergenic
GACGGTTACAATGTAGGCAT+GGG	0.570839	1.2:-2763932	MS.gene27345:CDS
TACGATGTGAGCTTGGTCGA+CGG	0.571799	1.2:-2763950	MS.gene27345:CDS
ATGTTTGTGGCGTGGCATGT+TGG	0.573358	1.2:+2763747	None:intergenic
CACCGGTGATTGTCAATATG+CGG	0.583056	1.2:-2763891	MS.gene27345:CDS
TCCGGTGGACTAAACTGTAT+CGG	0.584625	1.2:-2764043	MS.gene27345:CDS
GTTTGACTGCACCATCGGGT+TGG	0.589766	1.2:-2764141	MS.gene27345:CDS
TCAATATGCGGGGTGTGTTG+CGG	0.592968	1.2:-2763879	MS.gene27345:CDS
ATGGGTGTACGGGCTAGAGG+AGG	0.595042	1.2:-2763914	MS.gene27345:CDS
GCCAAATGGTGTAACTGCAT+TGG	0.596575	1.2:+2764239	None:intergenic
GTCAAACTAACTGATGTACC+GGG	0.599721	1.2:+2764157	None:intergenic
GTACGGGCTAGAGGAGGCAC+CGG	0.600853	1.2:-2763908	MS.gene27345:CDS
GGGGCTGCAATTCTCGGTCA+AGG	0.600916	1.2:-2764196	MS.gene27345:CDS
GGCATGGGTGTACGGGCTAG+AGG	0.603450	1.2:-2763917	MS.gene27345:CDS
TGTGTTGCGGACTTGAATGG+TGG	0.603671	1.2:-2763866	MS.gene27345:CDS
AGGTACCCCACCGGTAACAT+TGG	0.604332	1.2:-2764017	MS.gene27345:CDS
GGTGAACAGTGCAGGTTCGG+TGG	0.604516	1.2:-2763825	MS.gene27345:CDS
ATTGGATCAGCCGGTAATGG+TGG	0.605464	1.2:-2763983	MS.gene27345:CDS
AGGCAATTTAGCATCTGCTA+CGG	0.606331	1.2:-2764278	MS.gene27345:CDS
AATTCTGCCAATGTTACCGG+TGG	0.609607	1.2:+2764010	None:intergenic
ATCAGCCGGTAATGGTGGCA+AGG	0.611295	1.2:-2763978	MS.gene27345:CDS
TTCTGCCAATGTTACCGGTG+GGG	0.629018	1.2:+2764012	None:intergenic
GTCACAGGAGATTGTTCCGG+TGG	0.632624	1.2:-2764058	MS.gene27345:CDS
ATTCTGCCAATGTTACCGGT+GGG	0.634739	1.2:+2764011	None:intergenic
TGTGTGGTCGAACATGTTTG+TGG	0.637601	1.2:+2763734	None:intergenic
GGACTAAACTGTATCGGAGG+AGG	0.643072	1.2:-2764037	MS.gene27345:CDS
AAGCGTCCTGACCAACCCGA+TGG	0.645342	1.2:+2764130	None:intergenic
AAATATCTCGGAATAATGTG+TGG	0.647804	1.2:+2763718	None:intergenic
GTGTTTGGCGTTCAATACGG+AGG	0.667596	1.2:-2763786	MS.gene27345:CDS
GGTCGAACATGTTTGTGGCG+TGG	0.670223	1.2:+2763739	None:intergenic
GGGAACTCTTTCCGGCAACG+GGG	0.673252	1.2:-2764215	MS.gene27345:CDS
CCGGTGATTGTCAATATGCG+GGG	0.674634	1.2:-2763889	MS.gene27345:CDS
GCTGCAATTCTCGGTCAAGG+TGG	0.678285	1.2:-2764193	MS.gene27345:CDS
TCAAACTAACTGATGTACCG+GGG	0.689044	1.2:+2764158	None:intergenic
TATCGGAGGAGGTACCCCAC+CGG	0.691570	1.2:-2764026	MS.gene27345:CDS
GGTGGACTAAACTGTATCGG+AGG	0.699134	1.2:-2764040	MS.gene27345:CDS
ATGATCATGATGATCCATTG+GGG	0.705650	1.2:+2763603	None:intergenic
CAAACTAACTGATGTACCGG+GGG	0.713886	1.2:+2764159	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATGATTTTTAAAAAGACT+AGG	+	chr1.2:2763682-2763701	None:intergenic	15.0%
!!	AATGTTGTTTACCTAATAAG+AGG	+	chr1.2:2763657-2763676	None:intergenic	25.0%
!!!	TGCATGAATATATTTTTTGC+AGG	-	chr1.2:2763733-2763752	MS.gene27345:CDS	25.0%
	AAATATCTCGGAATAATGTG+TGG	+	chr1.2:2764316-2764335	None:intergenic	30.0%
	GCAAGCTTTCTTAAATATCT+CGG	+	chr1.2:2764328-2764347	None:intergenic	30.0%
	TTCAACATGTTCCTCTTATT+AGG	-	chr1.2:2763643-2763662	MS.gene27345:CDS	30.0%
!	TTGAAGAGAAGAAAGGTTTT+CGG	+	chr1.2:2763628-2763647	None:intergenic	30.0%
	AAGCATAACTGTAAGCAGTT+GGG	+	chr1.2:2764350-2764369	None:intergenic	35.0%
	ACATTGGCAGAATTCACTAT+TGG	-	chr1.2:2764030-2764049	MS.gene27345:CDS	35.0%
	AGTCAAACTAACTGATGTAC+CGG	+	chr1.2:2763878-2763897	None:intergenic	35.0%
	GAACATGTTGAAGAGAAGAA+AGG	+	chr1.2:2763635-2763654	None:intergenic	35.0%
	GCAAAACGTGATATGGTAAT+CGG	+	chr1.2:2764409-2764428	None:intergenic	35.0%
	TAAGCATAACTGTAAGCAGT+TGG	+	chr1.2:2764351-2764370	None:intergenic	35.0%
!	AGAAGAAAGGTTTTCGGATT+TGG	+	chr1.2:2763622-2763641	None:intergenic	35.0%
	ACCAATGCAGTTACACCATT+TGG	-	chr1.2:2763791-2763810	MS.gene27345:CDS	40.0%
	AGGCAATTTAGCATCTGCTA+CGG	-	chr1.2:2763753-2763772	MS.gene27345:CDS	40.0%
	GAATTCACTATTGGATCAGC+CGG	-	chr1.2:2764039-2764058	MS.gene27345:CDS	40.0%
	GTCAAACTAACTGATGTACC+GGG	+	chr1.2:2763877-2763896	None:intergenic	40.0%
	GTGAATTCTGCCAATGTTAC+CGG	+	chr1.2:2764027-2764046	None:intergenic	40.0%
	TCAAACTAACTGATGTACCG+GGG	+	chr1.2:2763876-2763895	None:intergenic	40.0%
!	AGTTAGTTTGACTGCACCAT+CGG	-	chr1.2:2763885-2763904	MS.gene27345:CDS	40.0%
!	GGTTTTCGGATTTGGATTCA+AGG	+	chr1.2:2763614-2763633	None:intergenic	40.0%
!!	GTAACTGCATTGGTTGTGTA+TGG	+	chr1.2:2763785-2763804	None:intergenic	40.0%
	AATTCTGCCAATGTTACCGG+TGG	+	chr1.2:2764024-2764043	None:intergenic	45.0%
	ACAATGTAGGCATGGGTGTA+CGG	-	chr1.2:2764106-2764125	MS.gene27345:CDS	45.0%
	ACCGGTGATTGTCAATATGC+GGG	-	chr1.2:2764141-2764160	MS.gene27345:CDS	45.0%
	ACTATTGGATCAGCCGGTAA+TGG	-	chr1.2:2764045-2764064	MS.gene27345:CDS	45.0%
	ATGCAGTTACACCATTTGGC+CGG	-	chr1.2:2763795-2763814	MS.gene27345:CDS	45.0%
	ATTCTGCCAATGTTACCGGT+GGG	+	chr1.2:2764023-2764042	None:intergenic	45.0%
	CAAACTAACTGATGTACCGG+GGG	+	chr1.2:2763875-2763894	None:intergenic	45.0%
	CACCGGTGATTGTCAATATG+CGG	-	chr1.2:2764140-2764159	MS.gene27345:CDS	45.0%
	CATTGGGGCAAAACGTGATA+TGG	+	chr1.2:2764416-2764435	None:intergenic	45.0%
	GACGGTTACAATGTAGGCAT+GGG	-	chr1.2:2764099-2764118	MS.gene27345:CDS	45.0%
	GCCAAATGGTGTAACTGCAT+TGG	+	chr1.2:2763795-2763814	None:intergenic	45.0%
	GGCAATGGTAAATGTGTCAC+AGG	-	chr1.2:2763958-2763977	MS.gene27345:CDS	45.0%
	TCCGATACAGTTTAGTCCAC+CGG	+	chr1.2:2763992-2764011	None:intergenic	45.0%
	TGTGTCACAGGAGATTGTTC+CGG	-	chr1.2:2763970-2763989	MS.gene27345:CDS	45.0%
	TGTGTGGTCGAACATGTTTG+TGG	+	chr1.2:2764300-2764319	None:intergenic	45.0%
	TTGGTCGACGGTTACAATGT+AGG	-	chr1.2:2764093-2764112	MS.gene27345:CDS	45.0%
!	CATATCACGTTTTGCCCCAA+TGG	-	chr1.2:2764414-2764433	MS.gene27345:CDS	45.0%
!	GTTAGTTTGACTGCACCATC+GGG	-	chr1.2:2763886-2763905	MS.gene27345:CDS	45.0%
!!	TCCGGTGGACTAAACTGTAT+CGG	-	chr1.2:2763988-2764007	MS.gene27345:CDS	45.0%
	ATGTTTGTGGCGTGGCATGT+TGG	+	chr1.2:2764287-2764306	None:intergenic	50.0%
	ATTTGGCCGGGAACTCTTTC+CGG	-	chr1.2:2763808-2763827	MS.gene27345:CDS	50.0%
	CAATGTAGGCATGGGTGTAC+GGG	-	chr1.2:2764107-2764126	MS.gene27345:CDS	50.0%
	CCCCGCATATTGACAATCAC+CGG	+	chr1.2:2764145-2764164	None:intergenic	50.0%
	CCGGTGATTGTCAATATGCG+GGG	-	chr1.2:2764142-2764161	MS.gene27345:CDS	50.0%
	CGACGGTTACAATGTAGGCA+TGG	-	chr1.2:2764098-2764117	MS.gene27345:CDS	50.0%
	CGCGTGTTTGGCGTTCAATA+CGG	-	chr1.2:2764242-2764261	MS.gene27345:CDS	50.0%
	GAAGTCCTTGCCACCATTAC+CGG	+	chr1.2:2764061-2764080	None:intergenic	50.0%
	GAATTTGACGGTGCAGGCAA+TGG	-	chr1.2:2763943-2763962	MS.gene27345:CDS	50.0%
	GGACTAAACTGTATCGGAGG+AGG	-	chr1.2:2763994-2764013	MS.gene27345:CDS	50.0%
	GGACTTCTACGATGTGAGCT+TGG	-	chr1.2:2764074-2764093	MS.gene27345:CDS	50.0%
	GTGTTTGGCGTTCAATACGG+AGG	-	chr1.2:2764245-2764264	MS.gene27345:CDS	50.0%
	TACGATGTGAGCTTGGTCGA+CGG	-	chr1.2:2764081-2764100	MS.gene27345:CDS	50.0%
	TAGGGTGAACAGTGCAGGTT+CGG	-	chr1.2:2764203-2764222	MS.gene27345:CDS	50.0%
	TCAAGCACTTGCACGTGTTC+TGG	-	chr1.2:2764381-2764400	MS.gene27345:intron	50.0%
	TCAATATGCGGGGTGTGTTG+CGG	-	chr1.2:2764152-2764171	MS.gene27345:CDS	50.0%
	TGCAGTTACACCATTTGGCC+GGG	-	chr1.2:2763796-2763815	MS.gene27345:CDS	50.0%
	TGTGTTGCGGACTTGAATGG+TGG	-	chr1.2:2764165-2764184	MS.gene27345:CDS	50.0%
	TTCTGCCAATGTTACCGGTG+GGG	+	chr1.2:2764022-2764041	None:intergenic	50.0%
!	ATTGGATCAGCCGGTAATGG+TGG	-	chr1.2:2764048-2764067	MS.gene27345:CDS	50.0%
!!	CGGACTGGTTGCGAATTTGA+CGG	-	chr1.2:2763931-2763950	MS.gene27345:CDS	50.0%
!!	GGACTTGAATGGTGGATGTC+CGG	-	chr1.2:2764173-2764192	MS.gene27345:CDS	50.0%
!!	GGTGGACTAAACTGTATCGG+AGG	-	chr1.2:2763991-2764010	MS.gene27345:CDS	50.0%
!!!	ACGGAGGAGTTTTGTTGCAC+CGG	-	chr1.2:2764261-2764280	MS.gene27345:CDS	50.0%
	AGGTACCCCACCGGTAACAT+TGG	-	chr1.2:2764014-2764033	MS.gene27345:CDS	55.0%
	CTGTTCACCCTAAGCTCTGC+CGG	+	chr1.2:2764195-2764214	None:intergenic	55.0%
	GAGCTTAGGGTGAACAGTGC+AGG	-	chr1.2:2764198-2764217	MS.gene27345:CDS	55.0%
	GCTGCAATTCTCGGTCAAGG+TGG	-	chr1.2:2763838-2763857	MS.gene27345:CDS	55.0%
	GGAAAGAGTTCCCGGCCAAA+TGG	+	chr1.2:2763809-2763828	None:intergenic	55.0%
	GGGTGTGTTGCGGACTTGAA+TGG	-	chr1.2:2764162-2764181	MS.gene27345:CDS	55.0%
	GGTCGAACATGTTTGTGGCG+TGG	+	chr1.2:2764295-2764314	None:intergenic	55.0%
	GGTTGCGAATTTGACGGTGC+AGG	-	chr1.2:2763937-2763956	MS.gene27345:CDS	55.0%
	GTCACAGGAGATTGTTCCGG+TGG	-	chr1.2:2763973-2763992	MS.gene27345:CDS	55.0%
	GTTTGACTGCACCATCGGGT+TGG	-	chr1.2:2763890-2763909	MS.gene27345:CDS	55.0%
	TGGATGTCCGGCAGAGCTTA+GGG	-	chr1.2:2764185-2764204	MS.gene27345:CDS	55.0%
!	ATCAGCCGGTAATGGTGGCA+AGG	-	chr1.2:2764053-2764072	MS.gene27345:CDS	55.0%
!!!	GGTGGTTTTTTGCTAGCCCC+CGG	-	chr1.2:2763856-2763875	MS.gene27345:CDS	55.0%
	AAGCGTCCTGACCAACCCGA+TGG	+	chr1.2:2763904-2763923	None:intergenic	60.0%
	CCGGGAACTCTTTCCGGCAA+CGG	-	chr1.2:2763814-2763833	MS.gene27345:CDS	60.0%
	CCGTTGCCGGAAAGAGTTCC+CGG	+	chr1.2:2763817-2763836	None:intergenic	60.0%
	CGGGAACTCTTTCCGGCAAC+GGG	-	chr1.2:2763815-2763834	MS.gene27345:CDS	60.0%
	GAGAATTGCAGCCCCGTTGC+CGG	+	chr1.2:2763830-2763849	None:intergenic	60.0%
	GGCAACGGGGCTGCAATTCT+CGG	-	chr1.2:2763829-2763848	MS.gene27345:CDS	60.0%
	GGGAACTCTTTCCGGCAACG+GGG	-	chr1.2:2763816-2763835	MS.gene27345:CDS	60.0%
	GGGGCTGCAATTCTCGGTCA+AGG	-	chr1.2:2763835-2763854	MS.gene27345:CDS	60.0%
	GGTGAACAGTGCAGGTTCGG+TGG	-	chr1.2:2764206-2764225	MS.gene27345:CDS	60.0%
	GTGGATGTCCGGCAGAGCTT+AGG	-	chr1.2:2764184-2764203	MS.gene27345:CDS	60.0%
	TTGTGGCGTGGCATGTTGGC+CGG	+	chr1.2:2764283-2764302	None:intergenic	60.0%
!	GGCGTGTAAGAGCGCGTGTT+TGG	-	chr1.2:2764230-2764249	MS.gene27345:CDS	60.0%
!!	ACTGCACCATCGGGTTGGTC+AGG	-	chr1.2:2763895-2763914	MS.gene27345:CDS	60.0%
!!	ATGGGTGTACGGGCTAGAGG+AGG	-	chr1.2:2764117-2764136	MS.gene27345:CDS	60.0%
!!	GAACAGTGCAGGTTCGGTGG+TGG	-	chr1.2:2764209-2764228	MS.gene27345:CDS	60.0%
!!	GGGTTGGTCAGGACGCTTCT+GGG	-	chr1.2:2763906-2763925	MS.gene27345:CDS	60.0%
!!	TATCGGAGGAGGTACCCCAC+CGG	-	chr1.2:2764005-2764024	MS.gene27345:CDS	60.0%
	GGTCAGGACGCTTCTGGGCT+CGG	-	chr1.2:2763911-2763930	MS.gene27345:CDS	65.0%
!!	CGGGTTGGTCAGGACGCTTC+TGG	-	chr1.2:2763905-2763924	MS.gene27345:CDS	65.0%
!!	GGCATGGGTGTACGGGCTAG+AGG	-	chr1.2:2764114-2764133	MS.gene27345:CDS	65.0%
!!	GTACGGGCTAGAGGAGGCAC+CGG	-	chr1.2:2764123-2764142	MS.gene27345:CDS	65.0%
	GGACGCTTCTGGGCTCGGAC+TGG	-	chr1.2:2763916-2763935	MS.gene27345:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	2763607	2764446	2763607	ID=MS.gene27345
chr1.2	mRNA	2763607	2764446	2763607	ID=MS.gene27345.t1;Parent=MS.gene27345
chr1.2	exon	2764389	2764446	2764389	ID=MS.gene27345.t1.exon1;Parent=MS.gene27345.t1
chr1.2	CDS	2764389	2764446	2764389	ID=cds.MS.gene27345.t1;Parent=MS.gene27345.t1
chr1.2	exon	2763607	2764298	2763607	ID=MS.gene27345.t1.exon2;Parent=MS.gene27345.t1
chr1.2	CDS	2763607	2764298	2763607	ID=cds.MS.gene27345.t1;Parent=MS.gene27345.t1

Gene Sequence

>MS.gene27345

ATGGCCTTGAATCCAAATCCGAAAACCTTTCTTCTCTTCAACATGTTCCTCTTATTAGGTAAACAACATTTACCTAGTCTTTTTAAAAATCATTTTAATTTTTTTATTTTTATAATTAATAGCTTATGCATGAATATATTTTTTGCAGGCAATTTAGCATCTGCTACGGTGTTTACCATACACAACCAATGCAGTTACACCATTTGGCCGGGAACTCTTTCCGGCAACGGGGCTGCAATTCTCGGTCAAGGTGGTTTTTTGCTAGCCCCCGGTACATCAGTTAGTTTGACTGCACCATCGGGTTGGTCAGGACGCTTCTGGGCTCGGACTGGTTGCGAATTTGACGGTGCAGGCAATGGTAAATGTGTCACAGGAGATTGTTCCGGTGGACTAAACTGTATCGGAGGAGGTACCCCACCGGTAACATTGGCAGAATTCACTATTGGATCAGCCGGTAATGGTGGCAAGGACTTCTACGATGTGAGCTTGGTCGACGGTTACAATGTAGGCATGGGTGTACGGGCTAGAGGAGGCACCGGTGATTGTCAATATGCGGGGTGTGTTGCGGACTTGAATGGTGGATGTCCGGCAGAGCTTAGGGTGAACAGTGCAGGTTCGGTGGTGGCGTGTAAGAGCGCGTGTTTGGCGTTCAATACGGAGGAGTTTTGTTGCACCGGCCAACATGCCACGCCACAAACATGTTCGACCACACATTATTCCGAGATATTTAAGAAAGCTTGCCCAACTGCTTACAGTTATGCTTATGATGATGCTTCAAGCACTTGCACGTGTTCTGGTTCCGATTACCATATCACGTTTTGCCCCAATGGATCATCATGA

Protein sequence

>MS.gene27345.t1

MALNPNPKTFLLFNMFLLLGNLASATVFTIHNQCSYTIWPGTLSGNGAAILGQGGFLLAPGTSVSLTAPSGWSGRFWARTGCEFDGAGNGKCVTGDCSGGLNCIGGGTPPVTLAEFTIGSAGNGGKDFYDVSLVDGYNVGMGVRARGGTGDCQYAGCVADLNGGCPAELRVNSAGSVVACKSACLAFNTEEFCCTGQHATPQTCSTTHYSEIFKKACPTAYSYAYDDASSTCTCSGSDYHITFCPNGSS