Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23980.t1 | XP_013468444.1 | 87.7 | 454 | 41 | 4 | 1 | 444 | 1 | 449 | 2.70E-179 | 638.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23980.t1 | F4IDQ5 | 29.1 | 237 | 107 | 8 | 203 | 435 | 267 | 446 | 1.3e-12 | 75.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23980.t1 | A0A072VLJ2 | 87.7 | 454 | 41 | 4 | 1 | 444 | 1 | 449 | 2.0e-179 | 638.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049397 | MS.gene23980 | 0.801097 | 1.06E-48 | -1.69E-46 |
| MS.gene049418 | MS.gene23980 | 0.84501 | 5.30E-59 | -1.69E-46 |
| MS.gene049596 | MS.gene23980 | 0.806558 | 7.76E-50 | -1.69E-46 |
| MS.gene049622 | MS.gene23980 | 0.813721 | 2.22E-51 | -1.69E-46 |
| MS.gene04985 | MS.gene23980 | 0.826092 | 3.28E-54 | -1.69E-46 |
| MS.gene049989 | MS.gene23980 | 0.903059 | 5.13E-79 | -1.69E-46 |
| MS.gene050297 | MS.gene23980 | 0.895405 | 9.89E-76 | -1.69E-46 |
| MS.gene050310 | MS.gene23980 | 0.828802 | 7.34E-55 | -1.69E-46 |
| MS.gene050648 | MS.gene23980 | 0.842638 | 2.29E-58 | -1.69E-46 |
| MS.gene051067 | MS.gene23980 | 0.829764 | 4.29E-55 | -1.69E-46 |
| MS.gene051094 | MS.gene23980 | 0.81375 | 2.19E-51 | -1.69E-46 |
| MS.gene051263 | MS.gene23980 | 0.865481 | 5.72E-65 | -1.69E-46 |
| MS.gene051478 | MS.gene23980 | 0.848648 | 5.36E-60 | -1.69E-46 |
| MS.gene051629 | MS.gene23980 | 0.857126 | 2.02E-62 | -1.69E-46 |
| MS.gene051648 | MS.gene23980 | 0.806598 | 7.61E-50 | -1.69E-46 |
| MS.gene051698 | MS.gene23980 | 0.800473 | 1.42E-48 | -1.69E-46 |
| MS.gene051742 | MS.gene23980 | 0.809179 | 2.15E-50 | -1.69E-46 |
| MS.gene051955 | MS.gene23980 | 0.805379 | 1.37E-49 | -1.69E-46 |
| MS.gene052143 | MS.gene23980 | 0.838099 | 3.52E-57 | -1.69E-46 |
| MS.gene052367 | MS.gene23980 | 0.858556 | 7.60E-63 | -1.69E-46 |
| MS.gene053416 | MS.gene23980 | 0.85816 | 9.97E-63 | -1.69E-46 |
| MS.gene053417 | MS.gene23980 | 0.859215 | 4.83E-63 | -1.69E-46 |
| MS.gene053536 | MS.gene23980 | 0.815565 | 8.66E-52 | -1.69E-46 |
| MS.gene053538 | MS.gene23980 | 0.81661 | 5.06E-52 | -1.69E-46 |
| MS.gene05369 | MS.gene23980 | 0.812469 | 4.17E-51 | -1.69E-46 |
| MS.gene053929 | MS.gene23980 | 0.808992 | 2.36E-50 | -1.69E-46 |
| MS.gene054551 | MS.gene23980 | 0.8045 | 2.10E-49 | -1.69E-46 |
| MS.gene054643 | MS.gene23980 | 0.853849 | 1.82E-61 | -1.69E-46 |
| MS.gene055411 | MS.gene23980 | 0.806274 | 8.91E-50 | -1.69E-46 |
| MS.gene055764 | MS.gene23980 | 0.822307 | 2.54E-53 | -1.69E-46 |
| MS.gene055957 | MS.gene23980 | 0.851212 | 1.03E-60 | -1.69E-46 |
| MS.gene056041 | MS.gene23980 | 0.851173 | 1.05E-60 | -1.69E-46 |
| MS.gene056042 | MS.gene23980 | 0.854249 | 1.40E-61 | -1.69E-46 |
| MS.gene056228 | MS.gene23980 | 0.81797 | 2.50E-52 | -1.69E-46 |
| MS.gene056335 | MS.gene23980 | 0.806556 | 7.77E-50 | -1.69E-46 |
| MS.gene056526 | MS.gene23980 | 0.815248 | 1.02E-51 | -1.69E-46 |
| MS.gene057004 | MS.gene23980 | 0.812659 | 3.79E-51 | -1.69E-46 |
| MS.gene057483 | MS.gene23980 | 0.817153 | 3.82E-52 | -1.69E-46 |
| MS.gene058196 | MS.gene23980 | 0.851299 | 9.71E-61 | -1.69E-46 |
| MS.gene058197 | MS.gene23980 | 0.87977 | 9.50E-70 | -1.69E-46 |
| MS.gene058198 | MS.gene23980 | 0.880132 | 7.06E-70 | -1.69E-46 |
| MS.gene058818 | MS.gene23980 | 0.829983 | 3.79E-55 | -1.69E-46 |
| MS.gene059300 | MS.gene23980 | 0.898809 | 3.69E-77 | -1.69E-46 |
| MS.gene059424 | MS.gene23980 | 0.810458 | 1.14E-50 | -1.69E-46 |
| MS.gene060473 | MS.gene23980 | 0.850512 | 1.62E-60 | -1.69E-46 |
| MS.gene060565 | MS.gene23980 | 0.855211 | 7.34E-62 | -1.69E-46 |
| MS.gene06057 | MS.gene23980 | 0.894589 | 2.14E-75 | -1.69E-46 |
| MS.gene061099 | MS.gene23980 | 0.820008 | 8.60E-53 | -1.69E-46 |
| MS.gene061260 | MS.gene23980 | 0.845813 | 3.21E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23980.t1 | MTR_1g069610 | 89.207 | 454 | 34 | 4 | 1 | 444 | 1 | 449 | 0.0 | 725 |
| MS.gene23980.t1 | MTR_3g450760 | 56.061 | 66 | 25 | 1 | 370 | 431 | 333 | 398 | 1.17e-15 | 79.0 |
| MS.gene23980.t1 | MTR_1g073670 | 28.076 | 317 | 185 | 8 | 1 | 305 | 1 | 286 | 2.65e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23980.t1 | AT1G04030 | 41.737 | 472 | 190 | 21 | 1 | 429 | 1 | 430 | 6.90e-73 | 236 |
| MS.gene23980.t1 | AT1G04030 | 41.737 | 472 | 190 | 21 | 1 | 429 | 1 | 430 | 6.90e-73 | 236 |
| MS.gene23980.t1 | AT5G44040 | 38.621 | 435 | 190 | 17 | 1 | 388 | 1 | 405 | 1.08e-60 | 203 |
| MS.gene23980.t1 | AT2G33400 | 29.167 | 312 | 150 | 11 | 1 | 305 | 1 | 248 | 6.14e-15 | 75.1 |
| MS.gene23980.t1 | AT1G06660 | 29.388 | 245 | 109 | 9 | 202 | 442 | 266 | 450 | 1.83e-14 | 75.9 |
| MS.gene23980.t1 | AT2G30820 | 53.425 | 73 | 28 | 3 | 372 | 441 | 326 | 395 | 1.90e-14 | 75.5 |
| MS.gene23980.t1 | AT2G30820 | 53.425 | 73 | 28 | 3 | 372 | 441 | 326 | 395 | 1.90e-14 | 75.5 |
Find 122 sgRNAs with CRISPR-Local
Find 157 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCACCTTCATCCTTTAA+AGG | 0.187029 | 1.3:-49063305 | MS.gene23980:CDS |
| ATTTCCTGGTTCAACTTCTT+TGG | 0.209021 | 1.3:+49063623 | None:intergenic |
| CCAGATGAGATGCCGATTAT+AGG | 0.229389 | 1.3:-49063365 | MS.gene23980:CDS |
| CTGTACTTGCTAGTTGTGTT+TGG | 0.276793 | 1.3:+49063278 | None:intergenic |
| TGAAGGAGTATGTAGGGTTT+TGG | 0.283066 | 1.3:+49064467 | None:intergenic |
| GAAAGGGATGTAAACTCTAT+TGG | 0.307848 | 1.3:-49063839 | MS.gene23980:CDS |
| TAGAGAAGAAGATGATGAAT+TGG | 0.315196 | 1.3:-49063972 | MS.gene23980:CDS |
| AAAGGGATGTAAACTCTATT+GGG | 0.318157 | 1.3:-49063838 | MS.gene23980:CDS |
| GCTGAATCCGGTTGAAAATA+TGG | 0.327930 | 1.3:-49063771 | MS.gene23980:CDS |
| ATTTCGTGTCAAGGAAATTT+CGG | 0.330928 | 1.3:-49063657 | MS.gene23980:CDS |
| TGGAATTCTAATGAGGGTTC+TGG | 0.341982 | 1.3:-49063326 | MS.gene23980:CDS |
| TGTGTTTGGAATTCCTTTAA+AGG | 0.345947 | 1.3:+49063292 | None:intergenic |
| CCTATAATCGGCATCTCATC+TGG | 0.351409 | 1.3:+49063365 | None:intergenic |
| TAGGAACAGTTGGGTCCTAT+TGG | 0.353133 | 1.3:-49063346 | MS.gene23980:CDS |
| TAGCATCAACTGGGATTTCC+TGG | 0.358117 | 1.3:+49063609 | None:intergenic |
| TTCGATAGGCTAGCATCAAC+TGG | 0.358795 | 1.3:+49063599 | None:intergenic |
| CACAAGAAGACGACTTTGAA+TGG | 0.373294 | 1.3:+49064149 | None:intergenic |
| ATACAATGTCCCTTTGGTTC+AGG | 0.374830 | 1.3:-49064496 | MS.gene23980:CDS |
| AAGAAGAAGTACTTGATGAT+TGG | 0.376669 | 1.3:-49064039 | MS.gene23980:CDS |
| TCGAATTGGTTGGGTTCATC+CGG | 0.379742 | 1.3:-49063581 | MS.gene23980:CDS |
| TGTTGTGAGATGCTCCTATC+AGG | 0.381760 | 1.3:+49063503 | None:intergenic |
| CTAAAATAGGCCTATCTTCA+AGG | 0.385904 | 1.3:+49063459 | None:intergenic |
| TTCTCTAGCCTTGTCTCAAA+TGG | 0.386868 | 1.3:+49063136 | None:intergenic |
| TCTGTGACTTCGAATTCAAC+TGG | 0.389893 | 1.3:-49064115 | MS.gene23980:CDS |
| TACAAACAAGCCTTGTTAAC+AGG | 0.398342 | 1.3:+49063548 | None:intergenic |
| ATCATAGGTATCAGAATTGT+AGG | 0.406684 | 1.3:-49064078 | MS.gene23980:CDS |
| CTCTTACAAGGAGACTTCCT+TGG | 0.412158 | 1.3:+49063389 | None:intergenic |
| TTCAGGCAAAACAGACTCAT+AGG | 0.416926 | 1.3:+49064207 | None:intergenic |
| CAATTCTGATACCTATGATT+AGG | 0.419539 | 1.3:+49064082 | None:intergenic |
| CAGTGATAAGCCTTGAAGAT+AGG | 0.427800 | 1.3:-49063469 | MS.gene23980:CDS |
| GACCCAACTGTTCCTATAAT+CGG | 0.428480 | 1.3:+49063353 | None:intergenic |
| GAAGACGACTTTGAATGGTT+TGG | 0.430474 | 1.3:+49064154 | None:intergenic |
| AGAAGAAGTACTTGATGATT+GGG | 0.432512 | 1.3:-49064038 | MS.gene23980:CDS |
| GGTCTTACCCTCCTAATCAT+AGG | 0.436853 | 1.3:-49064093 | MS.gene23980:CDS |
| TCTTACAAGGAGACTTCCTT+GGG | 0.437790 | 1.3:+49063390 | None:intergenic |
| GGAATTCCTTTAAAGGATGA+AGG | 0.441727 | 1.3:+49063299 | None:intergenic |
| ACCGTTAAAGCACCTAAAAT+AGG | 0.447229 | 1.3:+49063446 | None:intergenic |
| ATTCTAGTGAAGGAGTATGT+AGG | 0.448451 | 1.3:+49064460 | None:intergenic |
| TAGTGATGATGATGAGGAGG+AGG | 0.450003 | 1.3:-49064005 | MS.gene23980:CDS |
| TGCAGGATAAGAAGGTGAAT+TGG | 0.452750 | 1.3:-49063168 | MS.gene23980:intron |
| GGCTCAGTGGAAAGTTGTTA+AGG | 0.459239 | 1.3:-49063750 | MS.gene23980:CDS |
| GTGTAGGGATAAAACTGAAC+AGG | 0.461110 | 1.3:-49064314 | MS.gene23980:intron |
| AACAAGGCTTGTTTGTATGC+TGG | 0.461233 | 1.3:-49063542 | MS.gene23980:CDS |
| AGAGAAGAAGATGATGAATT+GGG | 0.461880 | 1.3:-49063971 | MS.gene23980:CDS |
| AACACTTCTGTCTCTAGCAT+TGG | 0.471678 | 1.3:+49063808 | None:intergenic |
| TGCTAGCCTATCGAATTGGT+TGG | 0.473010 | 1.3:-49063591 | MS.gene23980:CDS |
| TTGATGCTAGCCTATCGAAT+TGG | 0.473087 | 1.3:-49063595 | MS.gene23980:CDS |
| TGTTCATTCTGTGCTGAATC+CGG | 0.473897 | 1.3:-49063783 | MS.gene23980:CDS |
| GGAAAAGAGGAGGCTTTGGC+TGG | 0.475470 | 1.3:-49064178 | MS.gene23980:CDS |
| TTTACATCCCTTTCACATGT+AGG | 0.478384 | 1.3:+49063848 | None:intergenic |
| AATAGAGATGCTGCAGAAGC+TGG | 0.478976 | 1.3:-49063106 | MS.gene23980:CDS |
| ACAGCAATACAATGTCCCTT+TGG | 0.481214 | 1.3:-49064502 | MS.gene23980:CDS |
| GTCCTATTGGAATTCTAATG+AGG | 0.486281 | 1.3:-49063333 | MS.gene23980:CDS |
| AGGTGGAAAAGAGGAGGCTT+TGG | 0.488583 | 1.3:-49064182 | MS.gene23980:CDS |
| GCTAGCCTATCGAATTGGTT+GGG | 0.489992 | 1.3:-49063590 | MS.gene23980:CDS |
| GTGGCATCGGTACTCCTGAT+AGG | 0.491698 | 1.3:-49063517 | MS.gene23980:CDS |
| ATGCCGATTATAGGAACAGT+TGG | 0.502557 | 1.3:-49063356 | MS.gene23980:CDS |
| AGAGGTTTCATTTCGTGTCA+AGG | 0.504181 | 1.3:-49063666 | MS.gene23980:CDS |
| GTCAAGGAAATTTCGGAGGC+CGG | 0.506460 | 1.3:-49063650 | MS.gene23980:CDS |
| TCCTATGCCTACATGTGAAA+GGG | 0.507929 | 1.3:-49063855 | MS.gene23980:CDS |
| GTTCAACTTCTTTGGTGTGC+CGG | 0.508061 | 1.3:+49063631 | None:intergenic |
| TTCTGATACCTATGATTAGG+AGG | 0.509018 | 1.3:+49064085 | None:intergenic |
| GCCTGAAGAAGGTGGAAAAG+AGG | 0.511476 | 1.3:-49064191 | MS.gene23980:CDS |
| CTAAAAGAACACCACGATCA+AGG | 0.516379 | 1.3:-49063727 | MS.gene23980:CDS |
| ACTGGCTGTGCAGGATAAGA+AGG | 0.516689 | 1.3:-49063176 | MS.gene23980:intron |
| GATAAAACTGAACAGGAACT+TGG | 0.520043 | 1.3:-49064307 | MS.gene23980:CDS |
| TGATGATGATGAGGAGGAGG+AGG | 0.523004 | 1.3:-49064002 | MS.gene23980:CDS |
| TTGTATGCTGGTGGTGGCAT+CGG | 0.525729 | 1.3:-49063530 | MS.gene23980:CDS |
| GAAATGAAACCTCTGTATCA+TGG | 0.527422 | 1.3:+49063675 | None:intergenic |
| TTCTAGTGAAGGAGTATGTA+GGG | 0.527537 | 1.3:+49064461 | None:intergenic |
| CGAATTGGTTGGGTTCATCC+GGG | 0.529197 | 1.3:-49063580 | MS.gene23980:CDS |
| GAGTTTGAAGAAGACGGAAT+GGG | 0.529728 | 1.3:-49063944 | MS.gene23980:CDS |
| TCATGTGTTTACTGAGGAAG+TGG | 0.530102 | 1.3:-49063882 | MS.gene23980:CDS |
| TTGCTGTAGGGATAAAATTG+AGG | 0.530685 | 1.3:-49064284 | MS.gene23980:CDS |
| ATCTCATCTGGACTCTTACA+AGG | 0.536876 | 1.3:+49063377 | None:intergenic |
| GTTAACAGGTGTAGTTGTCC+CGG | 0.536876 | 1.3:+49063562 | None:intergenic |
| GATGATGAGGAGGAGGAGGA+TGG | 0.539564 | 1.3:-49063998 | MS.gene23980:CDS |
| AGAAGATGATGAATTGGGTG+TGG | 0.539828 | 1.3:-49063966 | MS.gene23980:CDS |
| GGAGTTTGAAGAAGACGGAA+TGG | 0.539838 | 1.3:-49063945 | MS.gene23980:CDS |
| ACCCTCATTAGAATTCCAAT+AGG | 0.541146 | 1.3:+49063331 | None:intergenic |
| CTAACAAAATTCTCTTTCTG+AGG | 0.541406 | 1.3:+49063704 | None:intergenic |
| GTCCTATGCCTACATGTGAA+AGG | 0.549863 | 1.3:-49063856 | MS.gene23980:CDS |
| ATAATTGCTTACTGTGATTG+TGG | 0.550999 | 1.3:+49064435 | None:intergenic |
| GACAACTACACCTGTTAACA+AGG | 0.558489 | 1.3:-49063558 | MS.gene23980:CDS |
| GCTTGTTTGTATGCTGGTGG+TGG | 0.559146 | 1.3:-49063536 | MS.gene23980:CDS |
| TTGGAGTGATCCTGAACCAA+AGG | 0.559802 | 1.3:+49064486 | None:intergenic |
| CTGTGACTTCGAATTCAACT+GGG | 0.563033 | 1.3:-49064114 | MS.gene23980:CDS |
| GGTGTGGAGTTTGAAGAAGA+CGG | 0.565341 | 1.3:-49063950 | MS.gene23980:CDS |
| TTACTTGAAGCACGTTGTTG+CGG | 0.569342 | 1.3:+49064732 | None:intergenic |
| CACACCAAAGAAGTTGAACC+AGG | 0.569677 | 1.3:-49063627 | MS.gene23980:CDS |
| TCCTATTGGAATTCTAATGA+GGG | 0.572382 | 1.3:-49063332 | MS.gene23980:CDS |
| TGGAGTGATCCTGAACCAAA+GGG | 0.574081 | 1.3:+49064487 | None:intergenic |
| TGCCGATTATAGGAACAGTT+GGG | 0.574954 | 1.3:-49063355 | MS.gene23980:CDS |
| ACAGGAACTTGGTTGCTGTA+GGG | 0.576162 | 1.3:-49064296 | MS.gene23980:CDS |
| TCTTTCTGAGGCCTTGATCG+TGG | 0.579965 | 1.3:+49063716 | None:intergenic |
| TCATAGGTATCAGAATTGTA+GGG | 0.580930 | 1.3:-49064077 | MS.gene23980:CDS |
| TGAAACCTCTGTATCATGGT+TGG | 0.594134 | 1.3:+49063679 | None:intergenic |
| CTTCCTTGGGGACGATGTAG+CGG | 0.603133 | 1.3:+49063403 | None:intergenic |
| ATGAACCCAACCAATTCGAT+AGG | 0.604616 | 1.3:+49063585 | None:intergenic |
| TTTCCGCTACATCGTCCCCA+AGG | 0.613077 | 1.3:-49063406 | MS.gene23980:CDS |
| GGGATACTCGAAGTCGAGGA+AGG | 0.614773 | 1.3:-49063924 | MS.gene23980:CDS |
| AACAGGAACTTGGTTGCTGT+AGG | 0.616293 | 1.3:-49064297 | MS.gene23980:CDS |
| AAAGGGACATTGTATTGCTG+TGG | 0.618091 | 1.3:+49064504 | None:intergenic |
| TCGTGTCAAGGAAATTTCGG+AGG | 0.626776 | 1.3:-49063654 | MS.gene23980:CDS |
| GAATGGGATACTCGAAGTCG+AGG | 0.634105 | 1.3:-49063928 | MS.gene23980:CDS |
| AAGGCTTGTTTGTATGCTGG+TGG | 0.635824 | 1.3:-49063539 | MS.gene23980:CDS |
| CTGTAGGGATAAAATTGAGG+AGG | 0.639832 | 1.3:-49064281 | MS.gene23980:CDS |
| TCTGATACCTATGATTAGGA+GGG | 0.641103 | 1.3:+49064086 | None:intergenic |
| TCTTAGTGATGATGATGAGG+AGG | 0.641753 | 1.3:-49064008 | MS.gene23980:CDS |
| TCCCTTTCACATGTAGGCAT+AGG | 0.644867 | 1.3:+49063854 | None:intergenic |
| GATTGTGGTGATTCTAGTGA+AGG | 0.644878 | 1.3:+49064450 | None:intergenic |
| AAGGGATGTAAACTCTATTG+GGG | 0.647688 | 1.3:-49063837 | MS.gene23980:CDS |
| ATGCTACGCCATTTGAGACA+AGG | 0.659616 | 1.3:-49063144 | MS.gene23980:CDS |
| TGATCTTAGTGATGATGATG+AGG | 0.661250 | 1.3:-49064011 | MS.gene23980:CDS |
| TCGATAGGCTAGCATCAACT+GGG | 0.664173 | 1.3:+49063600 | None:intergenic |
| TGAAGAAGGTGGAAAAGAGG+AGG | 0.666349 | 1.3:-49064188 | MS.gene23980:CDS |
| GATAGGAGCATCTCACAACA+AGG | 0.666708 | 1.3:-49063500 | MS.gene23980:CDS |
| TAGTACCAACCATGATACAG+AGG | 0.668642 | 1.3:-49063684 | MS.gene23980:CDS |
| CGGTTGAAAATATGGCTCAG+TGG | 0.681764 | 1.3:-49063763 | MS.gene23980:CDS |
| CACAACTAGCAAGTACAGAG+AGG | 0.701957 | 1.3:-49063273 | MS.gene23980:intron |
| TATTGATCATGTGTTTACTG+AGG | 0.741740 | 1.3:-49063888 | MS.gene23980:CDS |
| CTTACAAGGAGACTTCCTTG+GGG | 0.775099 | 1.3:+49063391 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAATAGAAAGTAAAAA+GGG | + | chr1.3:49063169-49063188 | None:intergenic | 15.0% |
| !! | GTAAAAGAAATTAAGATAAA+GGG | + | chr1.3:49063313-49063332 | None:intergenic | 15.0% |
| !! | TGTAAAAGAAATTAAGATAA+AGG | + | chr1.3:49063314-49063333 | None:intergenic | 15.0% |
| !!! | AAAATAGAGCTAAATTTTTT+AGG | - | chr1.3:49063274-49063293 | MS.gene23980:CDS | 15.0% |
| !! | AGAAAATAGAAAGTAAAAAG+GGG | + | chr1.3:49063168-49063187 | None:intergenic | 20.0% |
| !! | CAAGAAAATAGAAAGTAAAA+AGG | + | chr1.3:49063170-49063189 | None:intergenic | 20.0% |
| !!! | AGCTAAATTTTTTAGGTTTT+AGG | - | chr1.3:49063281-49063300 | MS.gene23980:CDS | 20.0% |
| !!! | TTCTTATTTGGATATTTAAC+TGG | - | chr1.3:49064666-49064685 | MS.gene23980:intron | 20.0% |
| ! | AAAATAGAAAGTAAAAAGGG+GGG | + | chr1.3:49063166-49063185 | None:intergenic | 25.0% |
| ! | ATTGGAAGTAAACAAAGAAA+AGG | + | chr1.3:49063240-49063259 | None:intergenic | 25.0% |
| ! | GAAAATAGAAAGTAAAAAGG+GGG | + | chr1.3:49063167-49063186 | None:intergenic | 25.0% |
| ! | TTGGAAGTAAACAAAGAAAA+GGG | + | chr1.3:49063239-49063258 | None:intergenic | 25.0% |
| !!! | ATTTTCACTCATTAGTTTGA+TGG | - | chr1.3:49064639-49064658 | MS.gene23980:intron | 25.0% |
| !!! | TGTTTTTTTGTTGTTGTGTA+GGG | - | chr1.3:49063531-49063550 | MS.gene23980:CDS | 25.0% |
| !!! | TTGTTTTTTTGTTGTTGTGT+AGG | - | chr1.3:49063530-49063549 | MS.gene23980:CDS | 25.0% |
| !!! | TTTGATGGTGAATTCTTATT+TGG | - | chr1.3:49064654-49064673 | MS.gene23980:intron | 25.0% |
| AAAGGGATGTAAACTCTATT+GGG | - | chr1.3:49064022-49064041 | MS.gene23980:CDS | 30.0% | |
| AAATAGAAAGTAAAAAGGGG+GGG | + | chr1.3:49063165-49063184 | None:intergenic | 30.0% | |
| AAGCATCCAAAAATAGAAAG+TGG | + | chr1.3:49063468-49063487 | None:intergenic | 30.0% | |
| AGAGAAGAAGATGATGAATT+GGG | - | chr1.3:49063889-49063908 | MS.gene23980:CDS | 30.0% | |
| ATAATTGCTTACTGTGATTG+TGG | + | chr1.3:49063428-49063447 | None:intergenic | 30.0% | |
| ATCATAGGTATCAGAATTGT+AGG | - | chr1.3:49063782-49063801 | MS.gene23980:CDS | 30.0% | |
| ATTTCGTGTCAAGGAAATTT+CGG | - | chr1.3:49064203-49064222 | MS.gene23980:CDS | 30.0% | |
| CAATTCTGATACCTATGATT+AGG | + | chr1.3:49063781-49063800 | None:intergenic | 30.0% | |
| CTAACAAAATTCTCTTTCTG+AGG | + | chr1.3:49064159-49064178 | None:intergenic | 30.0% | |
| TAGAGAAGAAGATGATGAAT+TGG | - | chr1.3:49063888-49063907 | MS.gene23980:CDS | 30.0% | |
| TATTGATCATGTGTTTACTG+AGG | - | chr1.3:49063972-49063991 | MS.gene23980:CDS | 30.0% | |
| TCATAGGTATCAGAATTGTA+GGG | - | chr1.3:49063783-49063802 | MS.gene23980:CDS | 30.0% | |
| TCCTATTGGAATTCTAATGA+GGG | - | chr1.3:49064528-49064547 | MS.gene23980:CDS | 30.0% | |
| ! | AAGACGAAGTGAATTTTTGT+AGG | - | chr1.3:49064612-49064631 | MS.gene23980:intron | 30.0% |
| ! | TGTGTTTGGAATTCCTTTAA+AGG | + | chr1.3:49064571-49064590 | None:intergenic | 30.0% |
| !! | AAGAAGAAGTACTTGATGAT+TGG | - | chr1.3:49063821-49063840 | MS.gene23980:CDS | 30.0% |
| !! | AGAAGAAGTACTTGATGATT+GGG | - | chr1.3:49063822-49063841 | MS.gene23980:CDS | 30.0% |
| AAGGGATGTAAACTCTATTG+GGG | - | chr1.3:49064023-49064042 | MS.gene23980:CDS | 35.0% | |
| ACCCTCATTAGAATTCCAAT+AGG | + | chr1.3:49064532-49064551 | None:intergenic | 35.0% | |
| ATTTCCTGGTTCAACTTCTT+TGG | + | chr1.3:49064240-49064259 | None:intergenic | 35.0% | |
| CTAAAATAGGCCTATCTTCA+AGG | + | chr1.3:49064404-49064423 | None:intergenic | 35.0% | |
| GAAAGGGATGTAAACTCTAT+TGG | - | chr1.3:49064021-49064040 | MS.gene23980:CDS | 35.0% | |
| GAAATGAAACCTCTGTATCA+TGG | + | chr1.3:49064188-49064207 | None:intergenic | 35.0% | |
| GATAAAACTGAACAGGAACT+TGG | - | chr1.3:49063553-49063572 | MS.gene23980:CDS | 35.0% | |
| GTCCTATTGGAATTCTAATG+AGG | - | chr1.3:49064527-49064546 | MS.gene23980:CDS | 35.0% | |
| TCTGATACCTATGATTAGGA+GGG | + | chr1.3:49063777-49063796 | None:intergenic | 35.0% | |
| TTCTGATACCTATGATTAGG+AGG | + | chr1.3:49063778-49063797 | None:intergenic | 35.0% | |
| TTGCTGTAGGGATAAAATTG+AGG | - | chr1.3:49063576-49063595 | MS.gene23980:CDS | 35.0% | |
| TTTACATCCCTTTCACATGT+AGG | + | chr1.3:49064015-49064034 | None:intergenic | 35.0% | |
| ! | ACCGTTAAAGCACCTAAAAT+AGG | + | chr1.3:49064417-49064436 | None:intergenic | 35.0% |
| ! | AGACTCATAGGTTTTCACAT+TGG | + | chr1.3:49063644-49063663 | None:intergenic | 35.0% |
| ! | ATTCTAGTGAAGGAGTATGT+AGG | + | chr1.3:49063403-49063422 | None:intergenic | 35.0% |
| ! | CACTCACCACTTTCTATTTT+TGG | - | chr1.3:49063459-49063478 | MS.gene23980:CDS | 35.0% |
| ! | CTTGAAGATAGGCCTATTTT+AGG | - | chr1.3:49064402-49064421 | MS.gene23980:intron | 35.0% |
| ! | GGAATTCCTTTAAAGGATGA+AGG | + | chr1.3:49064564-49064583 | None:intergenic | 35.0% |
| ! | TGATCTTAGTGATGATGATG+AGG | - | chr1.3:49063849-49063868 | MS.gene23980:CDS | 35.0% |
| ! | TTCTAGTGAAGGAGTATGTA+GGG | + | chr1.3:49063402-49063421 | None:intergenic | 35.0% |
| !! | GCCTATTTTAGGTGCTTTAA+CGG | - | chr1.3:49064413-49064432 | MS.gene23980:intron | 35.0% |
| !! | TACAAACAAGCCTTGTTAAC+AGG | + | chr1.3:49064315-49064334 | None:intergenic | 35.0% |
| !! | TATTTTAGGTGCTTTAACGG+TGG | - | chr1.3:49064416-49064435 | MS.gene23980:intron | 35.0% |
| !!! | TTGTTGCGGTTGTGTTTTTT+GGG | + | chr1.3:49063117-49063136 | None:intergenic | 35.0% |
| AAAGGGACATTGTATTGCTG+TGG | + | chr1.3:49063359-49063378 | None:intergenic | 40.0% | |
| AACACTTCTGTCTCTAGCAT+TGG | + | chr1.3:49064055-49064074 | None:intergenic | 40.0% | |
| ACAGCAATACAATGTCCCTT+TGG | - | chr1.3:49063358-49063377 | MS.gene23980:CDS | 40.0% | |
| AGAAGATGATGAATTGGGTG+TGG | - | chr1.3:49063894-49063913 | MS.gene23980:CDS | 40.0% | |
| AGAGGTTTCATTTCGTGTCA+AGG | - | chr1.3:49064194-49064213 | MS.gene23980:CDS | 40.0% | |
| ATCTCATCTGGACTCTTACA+AGG | + | chr1.3:49064486-49064505 | None:intergenic | 40.0% | |
| ATGAACCCAACCAATTCGAT+AGG | + | chr1.3:49064278-49064297 | None:intergenic | 40.0% | |
| CACAAGAAGACGACTTTGAA+TGG | + | chr1.3:49063714-49063733 | None:intergenic | 40.0% | |
| CAGTGATAAGCCTTGAAGAT+AGG | - | chr1.3:49064391-49064410 | MS.gene23980:intron | 40.0% | |
| CTAAAAGAACACCACGATCA+AGG | - | chr1.3:49064133-49064152 | MS.gene23980:CDS | 40.0% | |
| CTGTAGGGATAAAATTGAGG+AGG | - | chr1.3:49063579-49063598 | MS.gene23980:CDS | 40.0% | |
| CTGTGACTTCGAATTCAACT+GGG | - | chr1.3:49063746-49063765 | MS.gene23980:CDS | 40.0% | |
| GAAGACGACTTTGAATGGTT+TGG | + | chr1.3:49063709-49063728 | None:intergenic | 40.0% | |
| GACAACTACACCTGTTAACA+AGG | - | chr1.3:49064302-49064321 | MS.gene23980:CDS | 40.0% | |
| GACCCAACTGTTCCTATAAT+CGG | + | chr1.3:49064510-49064529 | None:intergenic | 40.0% | |
| GAGTTTGAAGAAGACGGAAT+GGG | - | chr1.3:49063916-49063935 | MS.gene23980:CDS | 40.0% | |
| GATTGTGGTGATTCTAGTGA+AGG | + | chr1.3:49063413-49063432 | None:intergenic | 40.0% | |
| GCTGAATCCGGTTGAAAATA+TGG | - | chr1.3:49064089-49064108 | MS.gene23980:CDS | 40.0% | |
| GGTTCACCTTCATCCTTTAA+AGG | - | chr1.3:49064555-49064574 | MS.gene23980:intron | 40.0% | |
| GTGTAGGGATAAAACTGAAC+AGG | - | chr1.3:49063546-49063565 | MS.gene23980:CDS | 40.0% | |
| TAGTACCAACCATGATACAG+AGG | - | chr1.3:49064176-49064195 | MS.gene23980:CDS | 40.0% | |
| TCATGTGTTTACTGAGGAAG+TGG | - | chr1.3:49063978-49063997 | MS.gene23980:CDS | 40.0% | |
| TCCTATGCCTACATGTGAAA+GGG | - | chr1.3:49064005-49064024 | MS.gene23980:CDS | 40.0% | |
| TCTGTGACTTCGAATTCAAC+TGG | - | chr1.3:49063745-49063764 | MS.gene23980:CDS | 40.0% | |
| TCTTACAAGGAGACTTCCTT+GGG | + | chr1.3:49064473-49064492 | None:intergenic | 40.0% | |
| TGAAACCTCTGTATCATGGT+TGG | + | chr1.3:49064184-49064203 | None:intergenic | 40.0% | |
| TGCAGGATAAGAAGGTGAAT+TGG | - | chr1.3:49064692-49064711 | MS.gene23980:intron | 40.0% | |
| TGGAATTCTAATGAGGGTTC+TGG | - | chr1.3:49064534-49064553 | MS.gene23980:intron | 40.0% | |
| TGTTCATTCTGTGCTGAATC+CGG | - | chr1.3:49064077-49064096 | MS.gene23980:CDS | 40.0% | |
| TTACTTGAAGCACGTTGTTG+CGG | + | chr1.3:49063131-49063150 | None:intergenic | 40.0% | |
| TTCAGGCAAAACAGACTCAT+AGG | + | chr1.3:49063656-49063675 | None:intergenic | 40.0% | |
| ! | AACAAGGCTTGTTTGTATGC+TGG | - | chr1.3:49064318-49064337 | MS.gene23980:intron | 40.0% |
| ! | ATACAATGTCCCTTTGGTTC+AGG | - | chr1.3:49063364-49063383 | MS.gene23980:CDS | 40.0% |
| ! | ATGCCGATTATAGGAACAGT+TGG | - | chr1.3:49064504-49064523 | MS.gene23980:CDS | 40.0% |
| ! | CACTGAGCCATATTTTCAAC+CGG | + | chr1.3:49064099-49064118 | None:intergenic | 40.0% |
| ! | CTGTACTTGCTAGTTGTGTT+TGG | + | chr1.3:49064585-49064604 | None:intergenic | 40.0% |
| ! | TCTTAGTGATGATGATGAGG+AGG | - | chr1.3:49063852-49063871 | MS.gene23980:CDS | 40.0% |
| ! | TGCCGATTATAGGAACAGTT+GGG | - | chr1.3:49064505-49064524 | MS.gene23980:CDS | 40.0% |
| ! | TTGATGCTAGCCTATCGAAT+TGG | - | chr1.3:49064265-49064284 | MS.gene23980:CDS | 40.0% |
| !! | GGTTGTTTTCTTGGTTGCTT+TGG | - | chr1.3:49063081-49063100 | MS.gene23980:CDS | 40.0% |
| !! | TGAAGGAGTATGTAGGGTTT+TGG | + | chr1.3:49063396-49063415 | None:intergenic | 40.0% |
| !! | TTCTCTAGCCTTGTCTCAAA+TGG | + | chr1.3:49064727-49064746 | None:intergenic | 40.0% |
| !! | TTTTGCTGCTGAGAAAGCAT+TGG | + | chr1.3:49063258-49063277 | None:intergenic | 40.0% |
| !!! | GTTGTGTTTTTTGGGTTTGG+AGG | + | chr1.3:49063109-49063128 | None:intergenic | 40.0% |
| !!! | GTTGTTGCGGTTGTGTTTTT+TGG | + | chr1.3:49063118-49063137 | None:intergenic | 40.0% |
| AACAGGAACTTGGTTGCTGT+AGG | - | chr1.3:49063563-49063582 | MS.gene23980:CDS | 45.0% | |
| AATAGAGATGCTGCAGAAGC+TGG | - | chr1.3:49064754-49064773 | MS.gene23980:CDS | 45.0% | |
| ACAGGAACTTGGTTGCTGTA+GGG | - | chr1.3:49063564-49063583 | MS.gene23980:CDS | 45.0% | |
| CACAACTAGCAAGTACAGAG+AGG | - | chr1.3:49064587-49064606 | MS.gene23980:intron | 45.0% | |
| CACACCAAAGAAGTTGAACC+AGG | - | chr1.3:49064233-49064252 | MS.gene23980:CDS | 45.0% | |
| CCTATAATCGGCATCTCATC+TGG | + | chr1.3:49064498-49064517 | None:intergenic | 45.0% | |
| CGGTTGAAAATATGGCTCAG+TGG | - | chr1.3:49064097-49064116 | MS.gene23980:CDS | 45.0% | |
| CTCTTACAAGGAGACTTCCT+TGG | + | chr1.3:49064474-49064493 | None:intergenic | 45.0% | |
| CTTACAAGGAGACTTCCTTG+GGG | + | chr1.3:49064472-49064491 | None:intergenic | 45.0% | |
| GGAGTTTGAAGAAGACGGAA+TGG | - | chr1.3:49063915-49063934 | MS.gene23980:CDS | 45.0% | |
| GGATATTTAACTGGCTGTGC+AGG | - | chr1.3:49064675-49064694 | MS.gene23980:intron | 45.0% | |
| GGCTCAGTGGAAAGTTGTTA+AGG | - | chr1.3:49064110-49064129 | MS.gene23980:CDS | 45.0% | |
| GGTCTTACCCTCCTAATCAT+AGG | - | chr1.3:49063767-49063786 | MS.gene23980:CDS | 45.0% | |
| GGTGTGGAGTTTGAAGAAGA+CGG | - | chr1.3:49063910-49063929 | MS.gene23980:CDS | 45.0% | |
| GTCCTATGCCTACATGTGAA+AGG | - | chr1.3:49064004-49064023 | MS.gene23980:CDS | 45.0% | |
| GTTAACAGGTGTAGTTGTCC+CGG | + | chr1.3:49064301-49064320 | None:intergenic | 45.0% | |
| TAGCATCAACTGGGATTTCC+TGG | + | chr1.3:49064254-49064273 | None:intergenic | 45.0% | |
| TAGGAACAGTTGGGTCCTAT+TGG | - | chr1.3:49064514-49064533 | MS.gene23980:CDS | 45.0% | |
| TCCCTTTCACATGTAGGCAT+AGG | + | chr1.3:49064009-49064028 | None:intergenic | 45.0% | |
| TCGAATTGGTTGGGTTCATC+CGG | - | chr1.3:49064279-49064298 | MS.gene23980:CDS | 45.0% | |
| TCGATAGGCTAGCATCAACT+GGG | + | chr1.3:49064263-49064282 | None:intergenic | 45.0% | |
| TCGTGTCAAGGAAATTTCGG+AGG | - | chr1.3:49064206-49064225 | MS.gene23980:CDS | 45.0% | |
| TGAAGAAGGTGGAAAAGAGG+AGG | - | chr1.3:49063672-49063691 | MS.gene23980:CDS | 45.0% | |
| TGGAGTGATCCTGAACCAAA+GGG | + | chr1.3:49063376-49063395 | None:intergenic | 45.0% | |
| TGTTGTGAGATGCTCCTATC+AGG | + | chr1.3:49064360-49064379 | None:intergenic | 45.0% | |
| TTCGATAGGCTAGCATCAAC+TGG | + | chr1.3:49064264-49064283 | None:intergenic | 45.0% | |
| TTGGAGTGATCCTGAACCAA+AGG | + | chr1.3:49063377-49063396 | None:intergenic | 45.0% | |
| ! | AAGGCTTGTTTGTATGCTGG+TGG | - | chr1.3:49064321-49064340 | MS.gene23980:intron | 45.0% |
| ! | ATGCTACGCCATTTGAGACA+AGG | - | chr1.3:49064716-49064735 | MS.gene23980:intron | 45.0% |
| ! | CCAGATGAGATGCCGATTAT+AGG | - | chr1.3:49064495-49064514 | MS.gene23980:CDS | 45.0% |
| ! | GAGTCTGTTTTGCCTGAAGA+AGG | - | chr1.3:49063658-49063677 | MS.gene23980:CDS | 45.0% |
| ! | GCTAGCCTATCGAATTGGTT+GGG | - | chr1.3:49064270-49064289 | MS.gene23980:CDS | 45.0% |
| ! | GTTCAACTTCTTTGGTGTGC+CGG | + | chr1.3:49064232-49064251 | None:intergenic | 45.0% |
| ! | TAGTGATGATGATGAGGAGG+AGG | - | chr1.3:49063855-49063874 | MS.gene23980:CDS | 45.0% |
| ! | TCCTCTTTTCCACCTTCTTC+AGG | + | chr1.3:49063673-49063692 | None:intergenic | 45.0% |
| ! | TCTGTTTTGCCTGAAGAAGG+TGG | - | chr1.3:49063661-49063680 | MS.gene23980:CDS | 45.0% |
| ! | TGCTAGCCTATCGAATTGGT+TGG | - | chr1.3:49064269-49064288 | MS.gene23980:CDS | 45.0% |
| !! | GATAGGAGCATCTCACAACA+AGG | - | chr1.3:49064360-49064379 | MS.gene23980:intron | 45.0% |
| !!! | GCGGTTGTGTTTTTTGGGTT+TGG | + | chr1.3:49063112-49063131 | None:intergenic | 45.0% |
| ACTGGCTGTGCAGGATAAGA+AGG | - | chr1.3:49064684-49064703 | MS.gene23980:intron | 50.0% | |
| AGGTGGAAAAGAGGAGGCTT+TGG | - | chr1.3:49063678-49063697 | MS.gene23980:CDS | 50.0% | |
| CGAATTGGTTGGGTTCATCC+GGG | - | chr1.3:49064280-49064299 | MS.gene23980:CDS | 50.0% | |
| GAATGGGATACTCGAAGTCG+AGG | - | chr1.3:49063932-49063951 | MS.gene23980:CDS | 50.0% | |
| GCCTGAAGAAGGTGGAAAAG+AGG | - | chr1.3:49063669-49063688 | MS.gene23980:CDS | 50.0% | |
| GCTTGTTTGTATGCTGGTGG+TGG | - | chr1.3:49064324-49064343 | MS.gene23980:intron | 50.0% | |
| GTCAAGGAAATTTCGGAGGC+CGG | - | chr1.3:49064210-49064229 | MS.gene23980:CDS | 50.0% | |
| ! | TGATGATGATGAGGAGGAGG+AGG | - | chr1.3:49063858-49063877 | MS.gene23980:CDS | 50.0% |
| !! | TCTTTCTGAGGCCTTGATCG+TGG | + | chr1.3:49064147-49064166 | None:intergenic | 50.0% |
| !! | TTGTATGCTGGTGGTGGCAT+CGG | - | chr1.3:49064330-49064349 | MS.gene23980:intron | 50.0% |
| CTTCCTTGGGGACGATGTAG+CGG | + | chr1.3:49064460-49064479 | None:intergenic | 55.0% | |
| GATGATGAGGAGGAGGAGGA+TGG | - | chr1.3:49063862-49063881 | MS.gene23980:CDS | 55.0% | |
| GGGATACTCGAAGTCGAGGA+AGG | - | chr1.3:49063936-49063955 | MS.gene23980:CDS | 55.0% | |
| TTTCCGCTACATCGTCCCCA+AGG | - | chr1.3:49064454-49064473 | MS.gene23980:CDS | 55.0% | |
| ! | GGAAAAGAGGAGGCTTTGGC+TGG | - | chr1.3:49063682-49063701 | MS.gene23980:CDS | 55.0% |
| !! | GTGGCATCGGTACTCCTGAT+AGG | - | chr1.3:49064343-49064362 | MS.gene23980:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 49063078 | 49064804 | 49063078 | ID=MS.gene23980 |
| chr1.3 | mRNA | 49063078 | 49064804 | 49063078 | ID=MS.gene23980.t1;Parent=MS.gene23980 |
| chr1.3 | exon | 49064736 | 49064804 | 49064736 | ID=MS.gene23980.t1.exon1;Parent=MS.gene23980.t1 |
| chr1.3 | CDS | 49064736 | 49064804 | 49064736 | ID=cds.MS.gene23980.t1;Parent=MS.gene23980.t1 |
| chr1.3 | exon | 49064447 | 49064547 | 49064447 | ID=MS.gene23980.t1.exon2;Parent=MS.gene23980.t1 |
| chr1.3 | CDS | 49064447 | 49064547 | 49064447 | ID=cds.MS.gene23980.t1;Parent=MS.gene23980.t1 |
| chr1.3 | exon | 49063274 | 49064330 | 49063274 | ID=MS.gene23980.t1.exon3;Parent=MS.gene23980.t1 |
| chr1.3 | CDS | 49063274 | 49064330 | 49063274 | ID=cds.MS.gene23980.t1;Parent=MS.gene23980.t1 |
| chr1.3 | exon | 49063078 | 49063185 | 49063078 | ID=MS.gene23980.t1.exon4;Parent=MS.gene23980.t1 |
| chr1.3 | CDS | 49063078 | 49063185 | 49063078 | ID=cds.MS.gene23980.t1;Parent=MS.gene23980.t1 |
| Gene Sequence |
| Protein sequence |
