Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24413.t1 | XP_003589185.1 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 5.00E-62 | 246.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24413.t1 | P97852 | 39.1 | 92 | 51 | 3 | 24 | 113 | 636 | 724 | 9.9e-11 | 67.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24413.t1 | G7I3K1 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 3.6e-62 | 246.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049786 | MS.gene24413 | 0.80712 | 5.90E-50 | -1.69E-46 |
| MS.gene050114 | MS.gene24413 | 0.818643 | 1.76E-52 | -1.69E-46 |
| MS.gene050288 | MS.gene24413 | 0.802862 | 4.59E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene24413 | 0.812713 | 3.69E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene24413 | 0.803331 | 3.67E-49 | -1.69E-46 |
| MS.gene051184 | MS.gene24413 | 0.800255 | 1.57E-48 | -1.69E-46 |
| MS.gene051245 | MS.gene24413 | 0.804434 | 2.16E-49 | -1.69E-46 |
| MS.gene051655 | MS.gene24413 | 0.815916 | 7.23E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene24413 | 0.814777 | 1.30E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene24413 | 0.809433 | 1.90E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene24413 | 0.805499 | 1.30E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene24413 | 0.8294 | 5.26E-55 | -1.69E-46 |
| MS.gene053381 | MS.gene24413 | 0.808283 | 3.34E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene24413 | 0.806241 | 9.05E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene24413 | 0.803357 | 3.62E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene24413 | 0.823416 | 1.40E-53 | -1.69E-46 |
| MS.gene055311 | MS.gene24413 | 0.804327 | 2.28E-49 | -1.69E-46 |
| MS.gene056181 | MS.gene24413 | 0.813611 | 2.35E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene24413 | 0.825064 | 5.75E-54 | -1.69E-46 |
| MS.gene056849 | MS.gene24413 | 0.813153 | 2.96E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene24413 | 0.82918 | 5.95E-55 | -1.69E-46 |
| MS.gene057999 | MS.gene24413 | 0.839018 | 2.04E-57 | -1.69E-46 |
| MS.gene058238 | MS.gene24413 | 0.804487 | 2.11E-49 | -1.69E-46 |
| MS.gene059050 | MS.gene24413 | 0.814044 | 1.88E-51 | -1.69E-46 |
| MS.gene059360 | MS.gene24413 | 0.812213 | 4.75E-51 | -1.69E-46 |
| MS.gene059830 | MS.gene24413 | 0.814526 | 1.47E-51 | -1.69E-46 |
| MS.gene059952 | MS.gene24413 | 0.803948 | 2.73E-49 | -1.69E-46 |
| MS.gene06010 | MS.gene24413 | 0.809782 | 1.60E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene24413 | 0.815714 | 8.03E-52 | -1.69E-46 |
| MS.gene060191 | MS.gene24413 | 0.810541 | 1.10E-50 | -1.69E-46 |
| MS.gene060297 | MS.gene24413 | 0.805963 | 1.04E-49 | -1.69E-46 |
| MS.gene060586 | MS.gene24413 | 0.803444 | 3.48E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24413.t1 | MTR_1g019430 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 8.01e-88 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24413.t1 | AT5G42890 | 79.508 | 122 | 25 | 0 | 4 | 125 | 2 | 123 | 1.17e-68 | 202 |
Find 21 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGGGTGCAATGAAGATTA+AGG | 0.247988 | 1.1:-3645442 | MS.gene24413:CDS |
| GCTCACATCTTGGAAGGCTT+TGG | 0.309953 | 1.1:+3645378 | None:intergenic |
| GACTTTGTTAAGGTTGCATT+AGG | 0.316740 | 1.1:-3645598 | MS.gene24413:CDS |
| CTCACATCTTGGAAGGCTTT+GGG | 0.353213 | 1.1:+3645379 | None:intergenic |
| TCAGTTTCAAATTGCTCCTA+AGG | 0.429160 | 1.1:-3646602 | MS.gene24413:intron |
| TACAGATGAAGACTTTGTTA+AGG | 0.431661 | 1.1:-3645608 | MS.gene24413:CDS |
| GAAGGCTTTGGGAAGATATC+CGG | 0.454252 | 1.1:+3645390 | None:intergenic |
| AGACATCTCTGCTCACATCT+TGG | 0.471053 | 1.1:+3645368 | None:intergenic |
| AGGGGTGCAATGAAGATTAA+GGG | 0.479063 | 1.1:-3645441 | MS.gene24413:CDS |
| ACTTTGTTAAGGTTGCATTA+GGG | 0.521418 | 1.1:-3645597 | MS.gene24413:CDS |
| TTCTCCTTTCTTGAGATCAA+CGG | 0.549718 | 1.1:+3646461 | None:intergenic |
| TATATAGGGGCATATGAAGG+TGG | 0.550169 | 1.1:-3645655 | MS.gene24413:intron |
| GTGTATATAGGGGCATATGA+AGG | 0.563217 | 1.1:-3645658 | MS.gene24413:intron |
| TATACCGTTGATCTCAAGAA+AGG | 0.565878 | 1.1:-3646465 | MS.gene24413:CDS |
| ATATAGGGGCATATGAAGGT+GGG | 0.567168 | 1.1:-3645654 | MS.gene24413:intron |
| ATCCACAGATTGCTTTCATG+AGG | 0.575757 | 1.1:-3645567 | MS.gene24413:intron |
| ATCTCTGCTCACATCTTGGA+AGG | 0.575766 | 1.1:+3645372 | None:intergenic |
| TGCTGCTCAGAAATTTACTC+CGG | 0.597894 | 1.1:-3645409 | MS.gene24413:CDS |
| AACCTCATGAAAGCAATCTG+TGG | 0.609703 | 1.1:+3645565 | None:intergenic |
| ATTCGATGATGAATCAGCCA+TGG | 0.641685 | 1.1:+3646714 | None:intergenic |
| AAGAAAGGAGAAGTTACCAA+AGG | 0.658788 | 1.1:-3646450 | MS.gene24413:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAATAATAAAGAAAAAAA+AGG | + | chr1.1:3645517-3645536 | None:intergenic | 10.0% |
| !!! | ATTTTAAATAAATCAAAATC+TGG | + | chr1.1:3646032-3646051 | None:intergenic | 10.0% |
| !!! | TATAGATTTTCATTATATTA+AGG | - | chr1.1:3645889-3645908 | MS.gene24413:intron | 10.0% |
| !!! | TATTTAAAATCTGAAAAAAT+AGG | - | chr1.1:3646042-3646061 | MS.gene24413:intron | 10.0% |
| !! | AAATATACTTCATATATGTT+TGG | - | chr1.1:3646208-3646227 | MS.gene24413:intron | 15.0% |
| !! | ATATATGAAGTATATTTGTA+GGG | + | chr1.1:3646205-3646224 | None:intergenic | 15.0% |
| !! | AAATAATCATACATTAAGGA+TGG | + | chr1.1:3646410-3646429 | None:intergenic | 20.0% |
| !! | AACCAAATAATCATACATTA+AGG | + | chr1.1:3646414-3646433 | None:intergenic | 20.0% |
| !! | ACAAGAAGAGATTATAATTT+AGG | + | chr1.1:3645569-3645588 | None:intergenic | 20.0% |
| !! | AGAAAAATGAAATTAACCTT+TGG | + | chr1.1:3645661-3645680 | None:intergenic | 20.0% |
| !! | ATCCTTAATGTATGATTATT+TGG | - | chr1.1:3646409-3646428 | MS.gene24413:intron | 20.0% |
| !! | ATTATTTGGTTGTGTATATA+GGG | - | chr1.1:3646423-3646442 | MS.gene24413:intron | 20.0% |
| !! | CATATATGAAGTATATTTGT+AGG | + | chr1.1:3646206-3646225 | None:intergenic | 20.0% |
| !! | GCATTTAATCACATAAAATA+TGG | + | chr1.1:3646078-3646097 | None:intergenic | 20.0% |
| !! | TGAATCAAATTGAATTGAAA+AGG | + | chr1.1:3645690-3645709 | None:intergenic | 20.0% |
| !! | TTTAATCACATAAAATATGG+AGG | + | chr1.1:3646075-3646094 | None:intergenic | 20.0% |
| !!! | ATTAAAATTAACCAAGTTGA+TGG | + | chr1.1:3646367-3646386 | None:intergenic | 20.0% |
| !!! | GTTGAAAGTATCTAATATTT+TGG | - | chr1.1:3645758-3645777 | MS.gene24413:intron | 20.0% |
| ! | AAAAAACTCACCGAAAAAAT+TGG | - | chr1.1:3645458-3645477 | MS.gene24413:intron | 25.0% |
| ! | ACAATTATGTTGGCAAAAAT+AGG | - | chr1.1:3645987-3646006 | MS.gene24413:intron | 25.0% |
| ! | AGCAAAATTATAACAGTTGT+TGG | + | chr1.1:3646150-3646169 | None:intergenic | 25.0% |
| ! | ATCCTAAAATCTTGACATTA+TGG | + | chr1.1:3645812-3645831 | None:intergenic | 25.0% |
| ! | CAATTATGTTGGCAAAAATA+GGG | - | chr1.1:3645988-3646007 | MS.gene24413:intron | 25.0% |
| ! | GATTATTTGGTTGTGTATAT+AGG | - | chr1.1:3646422-3646441 | MS.gene24413:intron | 25.0% |
| ! | TAAACAAAGAAAAGCCAAAA+CGG | + | chr1.1:3646589-3646608 | None:intergenic | 25.0% |
| ! | TAAAGAAAAAAAAGGACCTT+AGG | + | chr1.1:3645509-3645528 | None:intergenic | 25.0% |
| ! | TTATTTGGTTGTGTATATAG+GGG | - | chr1.1:3646424-3646443 | MS.gene24413:intron | 25.0% |
| !! | AACCATAATGTCAAGATTTT+AGG | - | chr1.1:3645807-3645826 | MS.gene24413:intron | 25.0% |
| !! | AAGGTAAAATGATTTGTGTT+TGG | - | chr1.1:3646289-3646308 | MS.gene24413:intron | 25.0% |
| !! | TTGTGAATTTTGCAGAAAAT+TGG | - | chr1.1:3645588-3645607 | MS.gene24413:CDS | 25.0% |
| !!! | ATAAACGAGACCAATTTTTT+CGG | + | chr1.1:3645471-3645490 | None:intergenic | 25.0% |
| !!! | ATTGTTCTGAAATTGCTTTA+AGG | - | chr1.1:3645736-3645755 | MS.gene24413:intron | 25.0% |
| AATAGATACAAGGAAGTGAA+TGG | + | chr1.1:3646613-3646632 | None:intergenic | 30.0% | |
| AATTGTAGTTACTGATGCAT+TGG | - | chr1.1:3645855-3645874 | MS.gene24413:intron | 30.0% | |
| ACTTTGTTAAGGTTGCATTA+GGG | - | chr1.1:3646495-3646514 | MS.gene24413:intron | 30.0% | |
| GTAAATGAAAAAGTTGCATC+TGG | + | chr1.1:3646467-3646486 | None:intergenic | 30.0% | |
| TACAGATGAAGACTTTGTTA+AGG | - | chr1.1:3646484-3646503 | MS.gene24413:CDS | 30.0% | |
| TGGATGAGATACAATTATGT+TGG | - | chr1.1:3645977-3645996 | MS.gene24413:intron | 30.0% | |
| ! | AATTTTGTCCCCTATAAAAG+TGG | - | chr1.1:3646330-3646349 | MS.gene24413:intron | 30.0% |
| ! | TAGATTATAGTTTGTGCTCT+AGG | - | chr1.1:3646110-3646129 | MS.gene24413:intron | 30.0% |
| ! | TATGACTAAAAGTGAGTTGA+AGG | - | chr1.1:3646270-3646289 | MS.gene24413:intron | 30.0% |
| !!! | ATGTTTTGGTCCACTTTTAT+AGG | + | chr1.1:3646343-3646362 | None:intergenic | 30.0% |
| !!! | TGTTCTCTTTTTTTCCGTTT+TGG | - | chr1.1:3646572-3646591 | MS.gene24413:intron | 30.0% |
| !!! | TGTTTTGGTCCACTTTTATA+GGG | + | chr1.1:3646342-3646361 | None:intergenic | 30.0% |
| AAGAAAGGAGAAGTTACCAA+AGG | - | chr1.1:3645642-3645661 | MS.gene24413:CDS | 35.0% | |
| GACTTTGTTAAGGTTGCATT+AGG | - | chr1.1:3646494-3646513 | MS.gene24413:intron | 35.0% | |
| GTTGGCAAAAATAGGGATTT+GGG | - | chr1.1:3645995-3646014 | MS.gene24413:intron | 35.0% | |
| TACATTAAGGATGGCATTGT+AGG | + | chr1.1:3646401-3646420 | None:intergenic | 35.0% | |
| TATACCGTTGATCTCAAGAA+AGG | - | chr1.1:3645627-3645646 | MS.gene24413:CDS | 35.0% | |
| TCACTTCCTTGTATCTATTG+TGG | - | chr1.1:3646614-3646633 | MS.gene24413:CDS | 35.0% | |
| TCAGTTTCAAATTGCTCCTA+AGG | - | chr1.1:3645490-3645509 | MS.gene24413:intron | 35.0% | |
| TGTTGGCAAAAATAGGGATT+TGG | - | chr1.1:3645994-3646013 | MS.gene24413:intron | 35.0% | |
| TTCTCCTTTCTTGAGATCAA+CGG | + | chr1.1:3645634-3645653 | None:intergenic | 35.0% | |
| !! | GTTTTGGTCCACTTTTATAG+GGG | + | chr1.1:3646341-3646360 | None:intergenic | 35.0% |
| AACCTCATGAAAGCAATCTG+TGG | + | chr1.1:3646530-3646549 | None:intergenic | 40.0% | |
| ACACCACCACAATAGATACA+AGG | + | chr1.1:3646623-3646642 | None:intergenic | 40.0% | |
| ACCAAAACATGCCATCAACT+TGG | - | chr1.1:3646353-3646372 | MS.gene24413:intron | 40.0% | |
| ATCCACAGATTGCTTTCATG+AGG | - | chr1.1:3646525-3646544 | MS.gene24413:intron | 40.0% | |
| CTTCCTTGTATCTATTGTGG+TGG | - | chr1.1:3646617-3646636 | MS.gene24413:CDS | 40.0% | |
| TGCTGCTCAGAAATTTACTC+CGG | - | chr1.1:3646683-3646702 | MS.gene24413:CDS | 40.0% | |
| ! | AGGGGTGCAATGAAGATTAA+GGG | - | chr1.1:3646651-3646670 | MS.gene24413:CDS | 40.0% |
| ! | ATATAGGGGCATATGAAGGT+GGG | - | chr1.1:3646438-3646457 | MS.gene24413:intron | 40.0% |
| ! | GTGTATATAGGGGCATATGA+AGG | - | chr1.1:3646434-3646453 | MS.gene24413:intron | 40.0% |
| ! | TATATAGGGGCATATGAAGG+TGG | - | chr1.1:3646437-3646456 | MS.gene24413:intron | 40.0% |
| ! | TGTGGTGGTGTATGAATACA+GGG | - | chr1.1:3646632-3646651 | MS.gene24413:CDS | 40.0% |
| ! | TTGTGGTGGTGTATGAATAC+AGG | - | chr1.1:3646631-3646650 | MS.gene24413:CDS | 40.0% |
| !!! | ACCAAGTTGATGGCATGTTT+TGG | + | chr1.1:3646357-3646376 | None:intergenic | 40.0% |
| ATCTGTGAGCGATTTGTGCA+TGG | - | chr1.1:3645957-3645976 | MS.gene24413:intron | 45.0% | |
| ! | CAGGGGTGCAATGAAGATTA+AGG | - | chr1.1:3646650-3646669 | MS.gene24413:CDS | 45.0% |
| ! | GTGGTGGTGTATGAATACAG+GGG | - | chr1.1:3646633-3646652 | MS.gene24413:CDS | 45.0% |
| ! | TAGTTTGTGCTCTAGGTAGC+TGG | - | chr1.1:3646117-3646136 | MS.gene24413:intron | 45.0% |
| !! | GAAGGCTTTGGGAAGATATC+CGG | + | chr1.1:3646705-3646724 | None:intergenic | 45.0% |
| !!! | GGTGAGTTTTTTGCCAGCGT+CGG | + | chr1.1:3645450-3645469 | None:intergenic | 50.0% |
| ! | CAACACTTTTCCACCGACGC+TGG | - | chr1.1:3645434-3645453 | MS.gene24413:CDS | 55.0% |
| !!! | GAGTTTTTTGCCAGCGTCGG+TGG | + | chr1.1:3645447-3645466 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 3645380 | 3646734 | 3645380 | ID=MS.gene24413 |
| chr1.1 | mRNA | 3645380 | 3646734 | 3645380 | ID=MS.gene24413.t1;Parent=MS.gene24413 |
| chr1.1 | exon | 3646603 | 3646734 | 3646603 | ID=MS.gene24413.t1.exon1;Parent=MS.gene24413.t1 |
| chr1.1 | CDS | 3646603 | 3646734 | 3646603 | ID=cds.MS.gene24413.t1;Parent=MS.gene24413.t1 |
| chr1.1 | exon | 3646451 | 3646511 | 3646451 | ID=MS.gene24413.t1.exon2;Parent=MS.gene24413.t1 |
| chr1.1 | CDS | 3646451 | 3646511 | 3646451 | ID=cds.MS.gene24413.t1;Parent=MS.gene24413.t1 |
| chr1.1 | exon | 3645568 | 3645670 | 3645568 | ID=MS.gene24413.t1.exon3;Parent=MS.gene24413.t1 |
| chr1.1 | CDS | 3645568 | 3645670 | 3645568 | ID=cds.MS.gene24413.t1;Parent=MS.gene24413.t1 |
| chr1.1 | exon | 3645380 | 3645461 | 3645380 | ID=MS.gene24413.t1.exon4;Parent=MS.gene24413.t1 |
| chr1.1 | CDS | 3645380 | 3645461 | 3645380 | ID=cds.MS.gene24413.t1;Parent=MS.gene24413.t1 |
| Gene Sequence |
| Protein sequence |
