Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24747.t1 | XP_024634011.1 | 95.4 | 109 | 4 | 1 | 1 | 108 | 1 | 109 | 3.60E-53 | 217.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24747.t1 | A0A396IWG2 | 95.4 | 109 | 4 | 1 | 1 | 108 | 1 | 109 | 2.6e-53 | 217.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049320 | MS.gene24747 | 0.80026 | 1.57E-48 | -1.69E-46 |
| MS.gene050577 | MS.gene24747 | 0.800979 | 1.12E-48 | -1.69E-46 |
| MS.gene050675 | MS.gene24747 | 0.814257 | 1.69E-51 | -1.69E-46 |
| MS.gene05080 | MS.gene24747 | 0.800221 | 1.60E-48 | -1.69E-46 |
| MS.gene050868 | MS.gene24747 | 0.801505 | 8.73E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene24747 | 0.804098 | 2.54E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene24747 | 0.814726 | 1.33E-51 | -1.69E-46 |
| MS.gene051509 | MS.gene24747 | 0.807017 | 6.21E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene24747 | 0.805725 | 1.16E-49 | -1.69E-46 |
| MS.gene051941 | MS.gene24747 | 0.818505 | 1.89E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene24747 | 0.84579 | 3.26E-59 | -1.69E-46 |
| MS.gene052503 | MS.gene24747 | 0.816835 | 4.51E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene24747 | 0.817248 | 3.64E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene24747 | 0.804666 | 1.94E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene24747 | 0.800224 | 1.59E-48 | -1.69E-46 |
| MS.gene053480 | MS.gene24747 | 0.828292 | 9.75E-55 | -1.69E-46 |
| MS.gene053632 | MS.gene24747 | 0.824473 | 7.92E-54 | -1.69E-46 |
| MS.gene053916 | MS.gene24747 | 0.812758 | 3.61E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene24747 | 0.818962 | 1.49E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene24747 | 0.801008 | 1.10E-48 | -1.69E-46 |
| MS.gene05589 | MS.gene24747 | 0.824902 | 6.27E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene24747 | 0.83658 | 8.61E-57 | -1.69E-46 |
| MS.gene056504 | MS.gene24747 | 0.829899 | 3.98E-55 | -1.69E-46 |
| MS.gene056657 | MS.gene24747 | 0.807792 | 4.25E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene24747 | 0.83064 | 2.62E-55 | -1.69E-46 |
| MS.gene057734 | MS.gene24747 | 0.817437 | 3.30E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene24747 | 0.80143 | 9.04E-49 | -1.69E-46 |
| MS.gene05913 | MS.gene24747 | 0.806396 | 8.40E-50 | -1.69E-46 |
| MS.gene059367 | MS.gene24747 | 0.829572 | 4.77E-55 | -1.69E-46 |
| MS.gene059455 | MS.gene24747 | 0.813716 | 2.22E-51 | -1.69E-46 |
| MS.gene059667 | MS.gene24747 | 0.804623 | 1.98E-49 | -1.69E-46 |
| MS.gene059674 | MS.gene24747 | 0.802581 | 5.24E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene24747 | 0.811348 | 7.32E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene24747 | 0.816703 | 4.82E-52 | -1.69E-46 |
| MS.gene060778 | MS.gene24747 | 0.827993 | 1.15E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene24747 | 0.818133 | 2.30E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene24747 | 0.809583 | 1.76E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24747.t1 | MTR_3g076960 | 95.370 | 108 | 4 | 1 | 1 | 107 | 1 | 108 | 8.91e-55 | 168 |
| MS.gene24747.t1 | MTR_3g076950 | 68.908 | 119 | 23 | 6 | 1 | 108 | 1 | 116 | 9.24e-42 | 133 |
| MS.gene24747.t1 | MTR_3g076950 | 68.908 | 119 | 23 | 6 | 1 | 108 | 1 | 116 | 9.44e-42 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACCCTCTCAATGATTTC+AGG | 0.169116 | 3.2:+68114656 | None:intergenic |
| TTGAAGGCCATCTCACCATT+TGG | 0.332611 | 3.2:+68114743 | None:intergenic |
| GGTTGTGGTGGTGGCGGCCA+TGG | 0.367830 | 3.2:+68114678 | None:intergenic |
| CCTTCGTGGGTAATGTTGAT+TGG | 0.388716 | 3.2:+68114553 | None:intergenic |
| CAACCCTCTCAATGATTTCA+GGG | 0.395577 | 3.2:+68114657 | None:intergenic |
| TGAAGGCCATCTCACCATTT+GGG | 0.418823 | 3.2:+68114744 | None:intergenic |
| TCTCAATGATTTCAGGGTTG+TGG | 0.432069 | 3.2:+68114663 | None:intergenic |
| CCATTTGACCTGTTCCTTCG+TGG | 0.432698 | 3.2:+68114539 | None:intergenic |
| ATAGACAACCCTCTTGTTGA+AGG | 0.438765 | 3.2:+68114727 | None:intergenic |
| TGATTTCAGGGTTGTGGTGG+TGG | 0.441325 | 3.2:+68114669 | None:intergenic |
| GTCACTCATGGAATACATTC+AGG | 0.444691 | 3.2:-68114517 | MS.gene24747:CDS |
| CATTTGACCTGTTCCTTCGT+GGG | 0.467646 | 3.2:+68114540 | None:intergenic |
| CAACAAGAGGGTTGTCTATG+AGG | 0.468986 | 3.2:-68114723 | MS.gene24747:CDS |
| CCCTTCGAACTTCTGAAATG+TGG | 0.470828 | 3.2:+68114789 | None:intergenic |
| AACATTACCCACGAAGGAAC+AGG | 0.491085 | 3.2:-68114547 | MS.gene24747:CDS |
| CATTGAGAGGGTTGAAGTGG+TGG | 0.491879 | 3.2:-68114648 | MS.gene24747:CDS |
| TATGAGGAGGATGCGATTGA+AGG | 0.492172 | 3.2:-68114707 | MS.gene24747:CDS |
| CCACGAAGGAACAGGTCAAA+TGG | 0.498880 | 3.2:-68114539 | MS.gene24747:CDS |
| GTACCTGAGGTTAGGCCCTA+TGG | 0.503566 | 3.2:-68114614 | MS.gene24747:CDS |
| CCACCATAGGGCCTAACCTC+AGG | 0.504816 | 3.2:+68114611 | None:intergenic |
| TTTCAGGGTTGTGGTGGTGG+CGG | 0.510562 | 3.2:+68114672 | None:intergenic |
| GAATACTCTCGCTACCCAAA+TGG | 0.533741 | 3.2:-68114758 | MS.gene24747:CDS |
| CCTTCGAACTTCTGAAATGT+GGG | 0.541962 | 3.2:+68114790 | None:intergenic |
| ATGAACGTGTACCTGAGGTT+AGG | 0.542173 | 3.2:-68114622 | MS.gene24747:CDS |
| CCCACATTTCAGAAGTTCGA+AGG | 0.543003 | 3.2:-68114790 | MS.gene24747:CDS |
| ACAGGTCAAATGGTCACTCA+TGG | 0.552434 | 3.2:-68114529 | MS.gene24747:CDS |
| CAATGATTTCAGGGTTGTGG+TGG | 0.569215 | 3.2:+68114666 | None:intergenic |
| GTAGATCACTTGACCACCAT+AGG | 0.569439 | 3.2:+68114598 | None:intergenic |
| AATGTGGGTGTGGTAGTCAT+TGG | 0.581040 | 3.2:+68114805 | None:intergenic |
| GGGTAGCGAGAGTATTCGCT+AGG | 0.581864 | 3.2:+68114764 | None:intergenic |
| AGTGATCTACGAAGAGGACG+TGG | 0.585316 | 3.2:-68114585 | MS.gene24747:CDS |
| TCGCTACCCAAATGGTGAGA+TGG | 0.585879 | 3.2:-68114750 | MS.gene24747:CDS |
| GTGAGATGGCCTTCAACAAG+AGG | 0.602330 | 3.2:-68114736 | MS.gene24747:CDS |
| TAGATCACTTGACCACCATA+GGG | 0.606041 | 3.2:+68114599 | None:intergenic |
| GAACTTCTGAAATGTGGGTG+TGG | 0.614497 | 3.2:+68114795 | None:intergenic |
| CCACATTTCAGAAGTTCGAA+GGG | 0.620439 | 3.2:-68114789 | MS.gene24747:CDS |
| CCTGAGGTTAGGCCCTATGG+TGG | 0.626513 | 3.2:-68114611 | MS.gene24747:CDS |
| ACAACCCTGAAATCATTGAG+AGG | 0.645359 | 3.2:-68114661 | MS.gene24747:CDS |
| CAAGAGGGTTGTCTATGAGG+AGG | 0.649483 | 3.2:-68114720 | MS.gene24747:CDS |
| CAACCCTGAAATCATTGAGA+GGG | 0.651875 | 3.2:-68114660 | MS.gene24747:CDS |
| GCGATTGAAGGATCACGCCA+TGG | 0.655981 | 3.2:-68114695 | MS.gene24747:CDS |
| TGAGATGGCCTTCAACAAGA+GGG | 0.673455 | 3.2:-68114735 | MS.gene24747:CDS |
| AATCATTGAGAGGGTTGAAG+TGG | 0.684831 | 3.2:-68114651 | MS.gene24747:CDS |
| TGGTCAAGTGATCTACGAAG+AGG | 0.694239 | 3.2:-68114591 | MS.gene24747:CDS |
| CCAATCAACATTACCCACGA+AGG | 0.702298 | 3.2:-68114553 | MS.gene24747:CDS |
| GGAATATGAACGTGTACCTG+AGG | 0.717370 | 3.2:-68114627 | MS.gene24747:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCATTGAGAGGGTTGAAG+TGG | - | chr3.2:68114675-68114694 | MS.gene24747:CDS | 40.0% | |
| ACAACCCTGAAATCATTGAG+AGG | - | chr3.2:68114665-68114684 | MS.gene24747:CDS | 40.0% | |
| ATAGACAACCCTCTTGTTGA+AGG | + | chr3.2:68114602-68114621 | None:intergenic | 40.0% | |
| CAACCCTCTCAATGATTTCA+GGG | + | chr3.2:68114672-68114691 | None:intergenic | 40.0% | |
| CAACCCTGAAATCATTGAGA+GGG | - | chr3.2:68114666-68114685 | MS.gene24747:CDS | 40.0% | |
| CCACATTTCAGAAGTTCGAA+GGG | - | chr3.2:68114537-68114556 | MS.gene24747:CDS | 40.0% | |
| GTCACTCATGGAATACATTC+AGG | - | chr3.2:68114809-68114828 | MS.gene24747:CDS | 40.0% | |
| TAGATCACTTGACCACCATA+GGG | + | chr3.2:68114730-68114749 | None:intergenic | 40.0% | |
| TCAACCCTCTCAATGATTTC+AGG | + | chr3.2:68114673-68114692 | None:intergenic | 40.0% | |
| TCTCAATGATTTCAGGGTTG+TGG | + | chr3.2:68114666-68114685 | None:intergenic | 40.0% | |
| !! | CCTTCGAACTTCTGAAATGT+GGG | + | chr3.2:68114539-68114558 | None:intergenic | 40.0% |
| AACATTACCCACGAAGGAAC+AGG | - | chr3.2:68114779-68114798 | MS.gene24747:CDS | 45.0% | |
| AATGTGGGTGTGGTAGTCAT+TGG | + | chr3.2:68114524-68114543 | None:intergenic | 45.0% | |
| ACAGGTCAAATGGTCACTCA+TGG | - | chr3.2:68114797-68114816 | MS.gene24747:CDS | 45.0% | |
| ATGAACGTGTACCTGAGGTT+AGG | - | chr3.2:68114704-68114723 | MS.gene24747:CDS | 45.0% | |
| CAACAAGAGGGTTGTCTATG+AGG | - | chr3.2:68114603-68114622 | MS.gene24747:CDS | 45.0% | |
| CAATGATTTCAGGGTTGTGG+TGG | + | chr3.2:68114663-68114682 | None:intergenic | 45.0% | |
| CATTTGACCTGTTCCTTCGT+GGG | + | chr3.2:68114789-68114808 | None:intergenic | 45.0% | |
| CCAATCAACATTACCCACGA+AGG | - | chr3.2:68114773-68114792 | MS.gene24747:CDS | 45.0% | |
| CCCACATTTCAGAAGTTCGA+AGG | - | chr3.2:68114536-68114555 | MS.gene24747:CDS | 45.0% | |
| CCTTCGTGGGTAATGTTGAT+TGG | + | chr3.2:68114776-68114795 | None:intergenic | 45.0% | |
| GAATACTCTCGCTACCCAAA+TGG | - | chr3.2:68114568-68114587 | MS.gene24747:CDS | 45.0% | |
| GGAATATGAACGTGTACCTG+AGG | - | chr3.2:68114699-68114718 | MS.gene24747:CDS | 45.0% | |
| GTAGATCACTTGACCACCAT+AGG | + | chr3.2:68114731-68114750 | None:intergenic | 45.0% | |
| TATGAGGAGGATGCGATTGA+AGG | - | chr3.2:68114619-68114638 | MS.gene24747:CDS | 45.0% | |
| TGAAGGCCATCTCACCATTT+GGG | + | chr3.2:68114585-68114604 | None:intergenic | 45.0% | |
| TGAGATGGCCTTCAACAAGA+GGG | - | chr3.2:68114591-68114610 | MS.gene24747:CDS | 45.0% | |
| TGGTCAAGTGATCTACGAAG+AGG | - | chr3.2:68114735-68114754 | MS.gene24747:CDS | 45.0% | |
| TTGAAGGCCATCTCACCATT+TGG | + | chr3.2:68114586-68114605 | None:intergenic | 45.0% | |
| !! | CCCTTCGAACTTCTGAAATG+TGG | + | chr3.2:68114540-68114559 | None:intergenic | 45.0% |
| !! | GAACTTCTGAAATGTGGGTG+TGG | + | chr3.2:68114534-68114553 | None:intergenic | 45.0% |
| AGTGATCTACGAAGAGGACG+TGG | - | chr3.2:68114741-68114760 | MS.gene24747:CDS | 50.0% | |
| CAAGAGGGTTGTCTATGAGG+AGG | - | chr3.2:68114606-68114625 | MS.gene24747:CDS | 50.0% | |
| CATTGAGAGGGTTGAAGTGG+TGG | - | chr3.2:68114678-68114697 | MS.gene24747:CDS | 50.0% | |
| CCACGAAGGAACAGGTCAAA+TGG | - | chr3.2:68114787-68114806 | MS.gene24747:CDS | 50.0% | |
| CCATTTGACCTGTTCCTTCG+TGG | + | chr3.2:68114790-68114809 | None:intergenic | 50.0% | |
| GTGAGATGGCCTTCAACAAG+AGG | - | chr3.2:68114590-68114609 | MS.gene24747:CDS | 50.0% | |
| TCGCTACCCAAATGGTGAGA+TGG | - | chr3.2:68114576-68114595 | MS.gene24747:CDS | 50.0% | |
| TGATTTCAGGGTTGTGGTGG+TGG | + | chr3.2:68114660-68114679 | None:intergenic | 50.0% | |
| GCGATTGAAGGATCACGCCA+TGG | - | chr3.2:68114631-68114650 | MS.gene24747:CDS | 55.0% | |
| GTACCTGAGGTTAGGCCCTA+TGG | - | chr3.2:68114712-68114731 | MS.gene24747:CDS | 55.0% | |
| !! | GGGTAGCGAGAGTATTCGCT+AGG | + | chr3.2:68114565-68114584 | None:intergenic | 55.0% |
| !! | TTTCAGGGTTGTGGTGGTGG+CGG | + | chr3.2:68114657-68114676 | None:intergenic | 55.0% |
| CCACCATAGGGCCTAACCTC+AGG | + | chr3.2:68114718-68114737 | None:intergenic | 60.0% | |
| CCTGAGGTTAGGCCCTATGG+TGG | - | chr3.2:68114715-68114734 | MS.gene24747:CDS | 60.0% | |
| !! | GGTTGTGGTGGTGGCGGCCA+TGG | + | chr3.2:68114651-68114670 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 68114511 | 68114837 | 68114511 | ID=MS.gene24747 |
| chr3.2 | mRNA | 68114511 | 68114837 | 68114511 | ID=MS.gene24747.t1;Parent=MS.gene24747 |
| chr3.2 | exon | 68114511 | 68114837 | 68114511 | ID=MS.gene24747.t1.exon1;Parent=MS.gene24747.t1 |
| chr3.2 | CDS | 68114511 | 68114837 | 68114511 | ID=cds.MS.gene24747.t1;Parent=MS.gene24747.t1 |
| Gene Sequence |
| Protein sequence |
