Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25648.t1 | XP_013450314.1 | 100 | 44 | 0 | 0 | 1 | 44 | 1 | 44 | 4.90E-16 | 92.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25648.t1 | O22518 | 97.7 | 43 | 1 | 0 | 2 | 44 | 10 | 52 | 1.7e-17 | 89.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25648.t1 | G8A187 | 100.0 | 44 | 0 | 0 | 1 | 44 | 1 | 44 | 3.5e-16 | 92.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049452 | MS.gene25648 | -0.809577 | 1.77E-50 | -1.69E-46 |
| MS.gene050105 | MS.gene25648 | -0.842475 | 2.53E-58 | -1.69E-46 |
| MS.gene050680 | MS.gene25648 | -0.819338 | 1.22E-52 | -1.69E-46 |
| MS.gene050750 | MS.gene25648 | -0.831703 | 1.44E-55 | -1.69E-46 |
| MS.gene050826 | MS.gene25648 | -0.808846 | 2.53E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene25648 | -0.833961 | 3.95E-56 | -1.69E-46 |
| MS.gene051122 | MS.gene25648 | -0.835893 | 1.29E-56 | -1.69E-46 |
| MS.gene051368 | MS.gene25648 | -0.817443 | 3.29E-52 | -1.69E-46 |
| MS.gene051924 | MS.gene25648 | 0.930334 | 1.90E-93 | -1.69E-46 |
| MS.gene051926 | MS.gene25648 | 0.979302 | 1.14E-147 | -1.69E-46 |
| MS.gene052190 | MS.gene25648 | -0.826561 | 2.54E-54 | -1.69E-46 |
| MS.gene052333 | MS.gene25648 | -0.843258 | 1.57E-58 | -1.69E-46 |
| MS.gene052519 | MS.gene25648 | -0.80084 | 1.19E-48 | -1.69E-46 |
| MS.gene052674 | MS.gene25648 | -0.847001 | 1.52E-59 | -1.69E-46 |
| MS.gene052675 | MS.gene25648 | -0.847861 | 8.84E-60 | -1.69E-46 |
| MS.gene052677 | MS.gene25648 | -0.853809 | 1.87E-61 | -1.69E-46 |
| MS.gene052965 | MS.gene25648 | 0.845211 | 4.68E-59 | -1.69E-46 |
| MS.gene053166 | MS.gene25648 | -0.836697 | 8.04E-57 | -1.69E-46 |
| MS.gene05415 | MS.gene25648 | -0.82275 | 2.00E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene25648 | 0.835832 | 1.33E-56 | -1.69E-46 |
| MS.gene054405 | MS.gene25648 | -0.835446 | 1.67E-56 | -1.69E-46 |
| MS.gene054468 | MS.gene25648 | -0.809267 | 2.06E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene25648 | -0.820053 | 8.40E-53 | -1.69E-46 |
| MS.gene054950 | MS.gene25648 | 0.802649 | 5.07E-49 | -1.69E-46 |
| MS.gene055230 | MS.gene25648 | -0.803974 | 2.70E-49 | -1.69E-46 |
| MS.gene055453 | MS.gene25648 | 0.834224 | 3.39E-56 | -1.69E-46 |
| MS.gene055455 | MS.gene25648 | 0.819903 | 9.10E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene25648 | -0.855695 | 5.31E-62 | -1.69E-46 |
| MS.gene055818 | MS.gene25648 | -0.807405 | 5.14E-50 | -1.69E-46 |
| MS.gene056480 | MS.gene25648 | -0.815005 | 1.15E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene25648 | -0.813604 | 2.35E-51 | -1.69E-46 |
| MS.gene056779 | MS.gene25648 | -0.845456 | 4.02E-59 | -1.69E-46 |
| MS.gene057026 | MS.gene25648 | -0.807336 | 5.31E-50 | -1.69E-46 |
| MS.gene057156 | MS.gene25648 | 0.866718 | 2.32E-65 | -1.69E-46 |
| MS.gene058223 | MS.gene25648 | 0.832805 | 7.67E-56 | -1.69E-46 |
| MS.gene059161 | MS.gene25648 | -0.825753 | 3.95E-54 | -1.69E-46 |
| MS.gene059952 | MS.gene25648 | -0.803659 | 3.14E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene25648 | -0.806252 | 9.00E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene25648 | -0.832864 | 7.41E-56 | -1.69E-46 |
| MS.gene060851 | MS.gene25648 | -0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene060852 | MS.gene25648 | -0.806 | 1.02E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene25648 | -0.802071 | 6.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25648.t1 | MTR_7g107400 | 100.000 | 44 | 0 | 0 | 1 | 44 | 1 | 44 | 3.42e-26 | 96.3 |
| MS.gene25648.t1 | MTR_3g095810 | 97.500 | 40 | 1 | 0 | 5 | 44 | 9 | 48 | 2.53e-22 | 85.9 |
| MS.gene25648.t1 | MTR_3g095810 | 97.500 | 40 | 1 | 0 | 5 | 44 | 9 | 48 | 2.54e-22 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25648.t1 | AT3G04770 | 80.952 | 42 | 8 | 0 | 3 | 44 | 9 | 50 | 2.05e-20 | 80.9 |
| MS.gene25648.t1 | AT3G04770 | 79.070 | 43 | 9 | 0 | 2 | 44 | 8 | 50 | 5.62e-20 | 80.1 |
| MS.gene25648.t1 | AT1G72370 | 76.744 | 43 | 10 | 0 | 2 | 44 | 7 | 49 | 4.52e-19 | 77.4 |
| MS.gene25648.t1 | AT1G72370 | 76.744 | 43 | 10 | 0 | 2 | 44 | 7 | 49 | 5.01e-19 | 77.4 |
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATTTAGGGTTTGGAAAA+TGG | 0.189223 | 7.1:+5953117 | None:intergenic |
| AAATTGGATAATTTAGGGTT+TGG | 0.349997 | 7.1:+5953110 | None:intergenic |
| CATCGGCGGCAAGCATCATC+TGG | 0.408335 | 7.1:+5953214 | None:intergenic |
| GGCAAGCATCATCTGGATAT+CGG | 0.457963 | 7.1:+5953221 | None:intergenic |
| ATCTTCAAGCGCCGTAACGA+TGG | 0.529765 | 7.1:-5953147 | MS.gene25648:CDS |
| ATGGAATGTTACCATCGTTA+CGG | 0.545997 | 7.1:+5953136 | None:intergenic |
| TTTAGTACCGAGATGGACAT+CGG | 0.561895 | 7.1:+5953197 | None:intergenic |
| AAGTTGACGAGGGGCAGCCA+TGG | 0.573667 | 7.1:+5953257 | None:intergenic |
| CTTTCTGAGAAAGTTGACGA+GGG | 0.588586 | 7.1:+5953247 | None:intergenic |
| CTTGCCGCCGATGTCCATCT+CGG | 0.588724 | 7.1:-5953204 | MS.gene25648:CDS |
| TCTTTCTGAGAAAGTTGACG+AGG | 0.624055 | 7.1:+5953246 | None:intergenic |
| TTTCTGAGAAAGTTGACGAG+GGG | 0.682900 | 7.1:+5953248 | None:intergenic |
| AGTACCGAGATGGACATCGG+CGG | 0.738432 | 7.1:+5953200 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGGAATGTTACCATCGTTA+CGG | + | chr7.1:5953244-5953263 | None:intergenic | 35.0% | |
| TAAAAACTGCGACTTTCAGA+TGG | - | chr7.1:5953199-5953218 | MS.gene25648:CDS | 35.0% | |
| ! | CTTTCTGAGAAAGTTGACGA+GGG | + | chr7.1:5953133-5953152 | None:intergenic | 40.0% |
| ! | TCTTTCTGAGAAAGTTGACG+AGG | + | chr7.1:5953134-5953153 | None:intergenic | 40.0% |
| ! | TTTAGTACCGAGATGGACAT+CGG | + | chr7.1:5953183-5953202 | None:intergenic | 40.0% |
| ! | TTTCTGAGAAAGTTGACGAG+GGG | + | chr7.1:5953132-5953151 | None:intergenic | 40.0% |
| GGCAAGCATCATCTGGATAT+CGG | + | chr7.1:5953159-5953178 | None:intergenic | 45.0% | |
| !!! | CGCAGTTTTTAGTACCGAGA+TGG | + | chr7.1:5953190-5953209 | None:intergenic | 45.0% |
| ! | ATCTTCAAGCGCCGTAACGA+TGG | - | chr7.1:5953230-5953249 | MS.gene25648:CDS | 50.0% |
| ! | AGTACCGAGATGGACATCGG+CGG | + | chr7.1:5953180-5953199 | None:intergenic | 55.0% |
| CATCGGCGGCAAGCATCATC+TGG | + | chr7.1:5953166-5953185 | None:intergenic | 60.0% | |
| CTTGCCGCCGATGTCCATCT+CGG | - | chr7.1:5953173-5953192 | MS.gene25648:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 5953122 | 5953277 | 5953122 | ID=MS.gene25648 |
| chr7.1 | mRNA | 5953122 | 5953277 | 5953122 | ID=MS.gene25648.t1;Parent=MS.gene25648 |
| chr7.1 | exon | 5953122 | 5953277 | 5953122 | ID=MS.gene25648.t1.exon1;Parent=MS.gene25648.t1 |
| chr7.1 | CDS | 5953122 | 5953277 | 5953122 | ID=cds.MS.gene25648.t1;Parent=MS.gene25648.t1 |
| Gene Sequence |
| Protein sequence |
