AlfalfaGEDB | MS.gene25648

M. sativa cultivar XinJiangDaYe / MS.gene25648

KEGG: K02998
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25648.t1	XP_013450314.1	100	44	0	0	1	44	1	44	4.90E-16	92.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25648.t1	O22518	97.7	43	1	0	2	44	10	52	1.7e-17	89.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25648.t1	G8A187	100.0	44	0	0	1	44	1	44	3.5e-16	92.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049452	MS.gene25648	-0.809577	1.77E-50	-1.69E-46
MS.gene050105	MS.gene25648	-0.842475	2.53E-58	-1.69E-46
MS.gene050680	MS.gene25648	-0.819338	1.22E-52	-1.69E-46
MS.gene050750	MS.gene25648	-0.831703	1.44E-55	-1.69E-46
MS.gene050826	MS.gene25648	-0.808846	2.53E-50	-1.69E-46
MS.gene050953	MS.gene25648	-0.833961	3.95E-56	-1.69E-46
MS.gene051122	MS.gene25648	-0.835893	1.29E-56	-1.69E-46
MS.gene051368	MS.gene25648	-0.817443	3.29E-52	-1.69E-46
MS.gene051924	MS.gene25648	0.930334	1.90E-93	-1.69E-46
MS.gene051926	MS.gene25648	0.979302	1.14E-147	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25648.t1	MTR_7g107400	100.000	44	0	1	44	1	44	3.42e-26	96.3
MS.gene25648.t1	MTR_3g095810	97.500	40	1	5	44	9	48	2.53e-22	85.9
MS.gene25648.t1	MTR_3g095810	97.500	40	1	5	44	9	48	2.54e-22	85.9

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25648.t1	AT3G04770	80.952	42	8	3	44	9	50	2.05e-20	80.9
MS.gene25648.t1	AT3G04770	79.070	43	9	2	44	8	50	5.62e-20	80.1
MS.gene25648.t1	AT1G72370	76.744	43	10	2	44	7	49	4.52e-19	77.4
MS.gene25648.t1	AT1G72370	76.744	43	10	2	44	7	49	5.01e-19	77.4

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Find 13 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTGGATAATTTAGGGTT+TGG	0.349997	7.1:+5953110	None:intergenic
AAGTTGACGAGGGGCAGCCA+TGG	0.573667	7.1:+5953257	None:intergenic
AGTACCGAGATGGACATCGG+CGG	0.738432	7.1:+5953200	None:intergenic
ATAATTTAGGGTTTGGAAAA+TGG	0.189223	7.1:+5953117	None:intergenic
ATCTTCAAGCGCCGTAACGA+TGG	0.529765	7.1:-5953147	MS.gene25648:CDS
ATGGAATGTTACCATCGTTA+CGG	0.545997	7.1:+5953136	None:intergenic
CATCGGCGGCAAGCATCATC+TGG	0.408335	7.1:+5953214	None:intergenic
CTTGCCGCCGATGTCCATCT+CGG	0.588724	7.1:-5953204	MS.gene25648:CDS
CTTTCTGAGAAAGTTGACGA+GGG	0.588586	7.1:+5953247	None:intergenic
GGCAAGCATCATCTGGATAT+CGG	0.457963	7.1:+5953221	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGGAATGTTACCATCGTTA+CGG	+	chr7.1:5953244-5953263	None:intergenic	35.0%
	TAAAAACTGCGACTTTCAGA+TGG	-	chr7.1:5953199-5953218	MS.gene25648:CDS	35.0%
	GGCAAGCATCATCTGGATAT+CGG	+	chr7.1:5953159-5953178	None:intergenic	45.0%
	CATCGGCGGCAAGCATCATC+TGG	+	chr7.1:5953166-5953185	None:intergenic	60.0%
	CTTGCCGCCGATGTCCATCT+CGG	-	chr7.1:5953173-5953192	MS.gene25648:CDS	60.0%
!	CTTTCTGAGAAAGTTGACGA+GGG	+	chr7.1:5953133-5953152	None:intergenic	40.0%
!	TCTTTCTGAGAAAGTTGACG+AGG	+	chr7.1:5953134-5953153	None:intergenic	40.0%
!	TTTAGTACCGAGATGGACAT+CGG	+	chr7.1:5953183-5953202	None:intergenic	40.0%
!	TTTCTGAGAAAGTTGACGAG+GGG	+	chr7.1:5953132-5953151	None:intergenic	40.0%
!	ATCTTCAAGCGCCGTAACGA+TGG	-	chr7.1:5953230-5953249	MS.gene25648:CDS	50.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	5953122	5953277	5953122	ID=MS.gene25648
chr7.1	mRNA	5953122	5953277	5953122	ID=MS.gene25648.t1;Parent=MS.gene25648
chr7.1	exon	5953122	5953277	5953122	ID=MS.gene25648.t1.exon1;Parent=MS.gene25648.t1
chr7.1	CDS	5953122	5953277	5953122	ID=cds.MS.gene25648.t1;Parent=MS.gene25648.t1

Gene Sequence	>MS.gene25648 ATGGCTGCCCCTCGTCAACTTTCTCAGAAAGAAGCCGATATCCAGATGATGCTTGCCGCCGATGTCCATCTCGGTACTAAAAACTGCGACTTTCAGATGGAAAGATACATCTTCAAGCGCCGTAACGATGGTAACATTCCATTTTCCAAACCCTAA
Protein sequence	>MS.gene25648.t1 MAAPRQLSQKEADIQMMLAADVHLGTKNCDFQMERYIFKRRNDGNIPFSKP