AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28623


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28623.t1 MTR_7g110880 98.276 232 3 1 1 231 58 289 2.27e-167 463
MS.gene28623.t1 MTR_1g116590 47.887 71 35 2 156 224 41 111 1.12e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28623.t1 AT4G01670 47.414 232 82 7 16 229 34 243 5.60e-41 140
MS.gene28623.t1 AT2G46940 43.860 228 91 7 3 228 60 252 1.79e-40 139
MS.gene28623.t1 AT3G62070 45.992 237 77 11 2 231 36 228 4.13e-40 137
MS.gene28623.t1 AT2G31560 41.489 94 28 2 156 222 102 195 8.76e-14 68.2
MS.gene28623.t1 AT2G31560 41.489 94 28 2 156 222 102 195 8.76e-14 68.2
MS.gene28623.t1 AT2G31560 41.489 94 28 2 156 222 102 195 8.76e-14 68.2
MS.gene28623.t1 AT2G43340 41.489 94 28 2 156 222 89 182 5.64e-13 65.5
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1
MS.gene28623.t1 AT1G05870 41.237 97 29 3 156 224 88 184 1.03e-12 65.1

Find 65 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATAGTTGGGAGAATTTA+TGG 0.098125 7.2:-4667383 MS.gene28623:CDS
CTCCGTCTTGGTGGCGTTGC+CGG 0.229266 7.2:+4667543 None:intergenic
TAGGAGTTTGTGTATGGATT+TGG 0.274372 7.2:-4667247 MS.gene28623:CDS
GTAATTCACCTATTGCTAAT+TGG 0.279198 7.2:-4667125 MS.gene28623:CDS
TTACAACCAGGGTTGTCTTC+TGG 0.287780 7.2:-4667600 MS.gene28623:CDS
GTTAAAGCTTGTAGAGATCT+TGG 0.319724 7.2:-4667219 MS.gene28623:CDS
AGGTAGTTGTTATTCGTTAC+CGG 0.352866 7.2:-4667562 MS.gene28623:CDS
AGTCGTGCCTTCACATCTCT+TGG 0.356137 7.2:+4666864 None:intergenic
AAATAGTTGGGAGAATTTAT+GGG 0.384282 7.2:-4667382 MS.gene28623:CDS
AGGAAAGTGGTTTGGGAGGT+AGG 0.387981 7.2:-4667449 MS.gene28623:CDS
ATCAGCTTCAAAACTTTCAA+TGG 0.388916 7.2:+4667677 None:intergenic
TGGGAATAGGAGTTTGTGTA+TGG 0.391612 7.2:-4667253 MS.gene28623:CDS
CTGGGGTGATGGTGATTACT+AGG 0.398778 7.2:-4667284 MS.gene28623:CDS
AGAAACAAAGGAAAGTGGTT+TGG 0.402405 7.2:-4667457 MS.gene28623:CDS
TTGGTTCCGGTGAGTGTTGT+TGG 0.406202 7.2:-4667516 MS.gene28623:CDS
GAAACAAAGGAAAGTGGTTT+GGG 0.410000 7.2:-4667456 MS.gene28623:CDS
TGGGTGTCATGAGCCGTATT+TGG 0.411466 7.2:+4666803 None:intergenic
GCCACCAAGACGGAGGAGTT+TGG 0.412050 7.2:-4667535 MS.gene28623:CDS
GTTTGGGAGGTAGGAGAAGA+AGG 0.412318 7.2:-4667440 MS.gene28623:CDS
AAACTCCTATTCCCACCTTT+AGG 0.424753 7.2:+4667261 None:intergenic
GGAGAGAGTGATCAGAGTAC+TGG 0.429926 7.2:-4667303 MS.gene28623:CDS
TCTGGCTCTGAAAATGAATC+AGG 0.430829 7.2:-4667582 MS.gene28623:CDS
ATAGCATCTGCCACCAAATA+CGG 0.446123 7.2:-4666816 MS.gene28623:CDS
CTAGGCCTAAAGGTGGGAAT+AGG 0.446339 7.2:-4667266 MS.gene28623:CDS
GAATGAGAAGTGTGTTTGAA+AGG 0.457694 7.2:-4667407 MS.gene28623:CDS
GTAGGAGAAGAAGGAGGAGA+AGG 0.459589 7.2:-4667431 MS.gene28623:CDS
TAGAGATCTTGGATTTGAGT+TGG 0.463756 7.2:-4667208 MS.gene28623:CDS
GAGATTCGCCAATTAGCAAT+AGG 0.470838 7.2:+4667117 None:intergenic
GTTTGAAAGGGGAAATAGTT+GGG 0.473057 7.2:-4667394 MS.gene28623:CDS
TTAGAGAATGGTGTGAATAG+TGG 0.473221 7.2:-4667324 MS.gene28623:CDS
GAGAAAGAGAAATTAGAGAA+TGG 0.488744 7.2:-4667336 MS.gene28623:CDS
AATGAGAAGTGTGTTTGAAA+GGG 0.496838 7.2:-4667406 MS.gene28623:CDS
ACCAAACTCCTCCGTCTTGG+TGG 0.498106 7.2:+4667534 None:intergenic
AAGACGGAGGAGTTTGGTTC+CGG 0.500715 7.2:-4667529 MS.gene28623:CDS
GAGAGAGTGATCAGAGTACT+GGG 0.504015 7.2:-4667302 MS.gene28623:CDS
TGTTTGAAAGGGGAAATAGT+TGG 0.505309 7.2:-4667395 MS.gene28623:CDS
ATTGCTAATTGGCGAATCTC+CGG 0.508950 7.2:-4667114 MS.gene28623:CDS
TTCACTCCAACAACACTCAC+CGG 0.546445 7.2:+4667510 None:intergenic
AATTGGCGAATCTCCGGTCC+CGG 0.547996 7.2:-4667108 MS.gene28623:intron
GGAACCAAACTCCTCCGTCT+TGG 0.552249 7.2:+4667531 None:intergenic
AGAAAAGAAACAAAGGAAAG+TGG 0.562594 7.2:-4667462 MS.gene28623:CDS
ATGGTGATTACTAGGCCTAA+AGG 0.564020 7.2:-4667276 MS.gene28623:CDS
AAATTGAGAATTACCGGGAC+CGG 0.564355 7.2:+4667095 None:intergenic
ATGAGAAGTGTGTTTGAAAG+GGG 0.566156 7.2:-4667405 MS.gene28623:CDS
TGAAGCAAGAAAAGAAACAA+AGG 0.581851 7.2:-4667469 MS.gene28623:CDS
ACGACTCAAAGTGTGGGCAC+AGG 0.585079 7.2:-4666847 MS.gene28623:CDS
ACAAAGGAAAGTGGTTTGGG+AGG 0.586780 7.2:-4667453 MS.gene28623:CDS
TGATGATCCAAGAGATGTGA+AGG 0.588464 7.2:-4666871 MS.gene28623:CDS
TGGGAGGTAGGAGAAGAAGG+AGG 0.588937 7.2:-4667437 MS.gene28623:CDS
TGAAGGCACGACTCAAAGTG+TGG 0.596523 7.2:-4666854 MS.gene28623:CDS
TGATCAGAGTACTGGGGTGA+TGG 0.602328 7.2:-4667295 MS.gene28623:CDS
GATGATCAGTTATTACAACC+AGG 0.603207 7.2:-4667612 MS.gene28623:CDS
GTGTCATGAGCCGTATTTGG+TGG 0.611631 7.2:+4666806 None:intergenic
TACCGGCAACGCCACCAAGA+CGG 0.614089 7.2:-4667545 MS.gene28623:CDS
GTGATTACTAGGCCTAAAGG+TGG 0.635360 7.2:-4667273 MS.gene28623:CDS
TCAGAGCCAGAAGACAACCC+TGG 0.636829 7.2:+4667594 None:intergenic
ATGATCAGTTATTACAACCA+GGG 0.642055 7.2:-4667611 MS.gene28623:CDS
ACACTTGATACTAGTAGTGG+TGG 0.643694 7.2:-4667147 MS.gene28623:CDS
TCAACACTTGATACTAGTAG+TGG 0.666554 7.2:-4667150 MS.gene28623:CDS
GAAGGCACGACTCAAAGTGT+GGG 0.668787 7.2:-4666853 MS.gene28623:CDS
TGATTACTAGGCCTAAAGGT+GGG 0.670590 7.2:-4667272 MS.gene28623:CDS
GGACAAGTTTGACATACACA+TGG 0.672211 7.2:+4667698 None:intergenic
CGGCAACGCCACCAAGACGG+AGG 0.684248 7.2:-4667542 MS.gene28623:CDS
GATGGTGGTGATGAAGCAGA+TGG 0.717318 7.2:-4667657 MS.gene28623:CDS
AGAGAGTGATCAGAGTACTG+GGG 0.764816 7.2:-4667301 MS.gene28623:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTGTATTATATTATGT+AGG - chr7.2:4667611-4667630 MS.gene28623:CDS 10.0%
!! ATAAACATTATAAGATTGTT+TGG - chr7.2:4667480-4667499 MS.gene28623:CDS 15.0%
!! AAAACAATTTATGACATGTT+CGG - chr7.2:4667514-4667533 MS.gene28623:CDS 20.0%
!!! TCAAATTTTGAATTAATCGA+AGG + chr7.2:4667590-4667609 None:intergenic 20.0%
! AAATAGTTGGGAGAATTTAT+GGG - chr7.2:4667123-4667142 MS.gene28623:CDS 25.0%
AACCAAAATTGAGAATTACC+GGG + chr7.2:4667418-4667437 None:intergenic 30.0%
AGAAAAGAAACAAAGGAAAG+TGG - chr7.2:4667043-4667062 MS.gene28623:intron 30.0%
ATCAGCTTCAAAACTTTCAA+TGG + chr7.2:4666831-4666850 None:intergenic 30.0%
ATGATCAGTTATTACAACCA+GGG - chr7.2:4666894-4666913 MS.gene28623:intron 30.0%
GAAATAGTTGGGAGAATTTA+TGG - chr7.2:4667122-4667141 MS.gene28623:CDS 30.0%
GAACCAAAATTGAGAATTAC+CGG + chr7.2:4667419-4667438 None:intergenic 30.0%
GAGAAAGAGAAATTAGAGAA+TGG - chr7.2:4667169-4667188 MS.gene28623:CDS 30.0%
GTAATTCACCTATTGCTAAT+TGG - chr7.2:4667380-4667399 MS.gene28623:CDS 30.0%
TAAGCGCGTATCATATATTA+TGG + chr7.2:4667558-4667577 None:intergenic 30.0%
TGAAGCAAGAAAAGAAACAA+AGG - chr7.2:4667036-4667055 MS.gene28623:intron 30.0%
! AATGAGAAGTGTGTTTGAAA+GGG - chr7.2:4667099-4667118 MS.gene28623:intron 30.0%
! CTCAAATAAGTTGACCTAAT+CGG + chr7.2:4667448-4667467 None:intergenic 30.0%
! TTTGGTTCATATTTCCGATT+AGG - chr7.2:4667431-4667450 MS.gene28623:CDS 30.0%
!!! ATTGAAAGTTTTGAAGCTGA+TGG - chr7.2:4666830-4666849 MS.gene28623:CDS 30.0%
AGAAACAAAGGAAAGTGGTT+TGG - chr7.2:4667048-4667067 MS.gene28623:intron 35.0%
GAAACAAAGGAAAGTGGTTT+GGG - chr7.2:4667049-4667068 MS.gene28623:intron 35.0%
GATGATCAGTTATTACAACC+AGG - chr7.2:4666893-4666912 MS.gene28623:intron 35.0%
GTTAAAGCTTGTAGAGATCT+TGG - chr7.2:4667286-4667305 MS.gene28623:CDS 35.0%
GTTTGAAAGGGGAAATAGTT+GGG - chr7.2:4667111-4667130 MS.gene28623:CDS 35.0%
TAGAGATCTTGGATTTGAGT+TGG - chr7.2:4667297-4667316 MS.gene28623:CDS 35.0%
TAGGAGTTTGTGTATGGATT+TGG - chr7.2:4667258-4667277 MS.gene28623:CDS 35.0%
TCAACACTTGATACTAGTAG+TGG - chr7.2:4667355-4667374 MS.gene28623:CDS 35.0%
TGTTTGAAAGGGGAAATAGT+TGG - chr7.2:4667110-4667129 MS.gene28623:CDS 35.0%
TTAGAGAATGGTGTGAATAG+TGG - chr7.2:4667181-4667200 MS.gene28623:CDS 35.0%
! AGGTAGTTGTTATTCGTTAC+CGG - chr7.2:4666943-4666962 MS.gene28623:intron 35.0%
! ATGAGAAGTGTGTTTGAAAG+GGG - chr7.2:4667100-4667119 MS.gene28623:intron 35.0%
! GAATGAGAAGTGTGTTTGAA+AGG - chr7.2:4667098-4667117 MS.gene28623:intron 35.0%
AAACTCCTATTCCCACCTTT+AGG + chr7.2:4667247-4667266 None:intergenic 40.0%
AAATTGAGAATTACCGGGAC+CGG + chr7.2:4667413-4667432 None:intergenic 40.0%
ACACTTGATACTAGTAGTGG+TGG - chr7.2:4667358-4667377 MS.gene28623:CDS 40.0%
ATAGCATCTGCCACCAAATA+CGG - chr7.2:4667689-4667708 MS.gene28623:CDS 40.0%
ATGGTGATTACTAGGCCTAA+AGG - chr7.2:4667229-4667248 MS.gene28623:CDS 40.0%
ATTGCTAATTGGCGAATCTC+CGG - chr7.2:4667391-4667410 MS.gene28623:CDS 40.0%
GAGATTCGCCAATTAGCAAT+AGG + chr7.2:4667391-4667410 None:intergenic 40.0%
TGATGATCCAAGAGATGTGA+AGG - chr7.2:4667634-4667653 MS.gene28623:CDS 40.0%
TGATTACTAGGCCTAAAGGT+GGG - chr7.2:4667233-4667252 MS.gene28623:CDS 40.0%
TGGGAATAGGAGTTTGTGTA+TGG - chr7.2:4667252-4667271 MS.gene28623:CDS 40.0%
! GATGGTGAATTTTCTGCTGA+TGG - chr7.2:4666866-4666885 MS.gene28623:CDS 40.0%
! GTCCCGGTAATTCTCAATTT+TGG - chr7.2:4667413-4667432 MS.gene28623:CDS 40.0%
!! TCTGGCTCTGAAAATGAATC+AGG - chr7.2:4666923-4666942 MS.gene28623:intron 40.0%
!!! GAAAGTTTTGAAGCTGATGG+TGG - chr7.2:4666833-4666852 MS.gene28623:CDS 40.0%
GTGATTACTAGGCCTAAAGG+TGG - chr7.2:4667232-4667251 MS.gene28623:CDS 45.0%
TTACAACCAGGGTTGTCTTC+TGG - chr7.2:4666905-4666924 MS.gene28623:intron 45.0%
TTCACTCCAACAACACTCAC+CGG + chr7.2:4666998-4667017 None:intergenic 45.0%
! ACAAAGGAAAGTGGTTTGGG+AGG - chr7.2:4667052-4667071 MS.gene28623:intron 45.0%
! GAGAGAGTGATCAGAGTACT+GGG - chr7.2:4667203-4667222 MS.gene28623:CDS 45.0%
!! AGAGAGTGATCAGAGTACTG+GGG - chr7.2:4667204-4667223 MS.gene28623:CDS 45.0%
AGTCGTGCCTTCACATCTCT+TGG + chr7.2:4667644-4667663 None:intergenic 50.0%
CTAGGCCTAAAGGTGGGAAT+AGG - chr7.2:4667239-4667258 MS.gene28623:CDS 50.0%
GGAGAGAGTGATCAGAGTAC+TGG - chr7.2:4667202-4667221 MS.gene28623:CDS 50.0%
GTAGGAGAAGAAGGAGGAGA+AGG - chr7.2:4667074-4667093 MS.gene28623:intron 50.0%
GTTTGGGAGGTAGGAGAAGA+AGG - chr7.2:4667065-4667084 MS.gene28623:intron 50.0%
! AAGACGGAGGAGTTTGGTTC+CGG - chr7.2:4666976-4666995 MS.gene28623:intron 50.0%
! AGGAAAGTGGTTTGGGAGGT+AGG - chr7.2:4667056-4667075 MS.gene28623:intron 50.0%
! CTGGGGTGATGGTGATTACT+AGG - chr7.2:4667221-4667240 MS.gene28623:CDS 50.0%
! GAAGGCACGACTCAAAGTGT+GGG - chr7.2:4667652-4667671 MS.gene28623:CDS 50.0%
! GATGGTGGTGATGAAGCAGA+TGG - chr7.2:4666848-4666867 MS.gene28623:CDS 50.0%
! TGAAGGCACGACTCAAAGTG+TGG - chr7.2:4667651-4667670 MS.gene28623:CDS 50.0%
! TTGGTTCCGGTGAGTGTTGT+TGG - chr7.2:4666989-4667008 MS.gene28623:intron 50.0%
!! TGATCAGAGTACTGGGGTGA+TGG - chr7.2:4667210-4667229 MS.gene28623:CDS 50.0%
AATTGGCGAATCTCCGGTCC+CGG - chr7.2:4667397-4667416 MS.gene28623:CDS 55.0%
ACCAAACTCCTCCGTCTTGG+TGG + chr7.2:4666974-4666993 None:intergenic 55.0%
ACGACTCAAAGTGTGGGCAC+AGG - chr7.2:4667658-4667677 MS.gene28623:CDS 55.0%
GGAACCAAACTCCTCCGTCT+TGG + chr7.2:4666977-4666996 None:intergenic 55.0%
TCAGAGCCAGAAGACAACCC+TGG + chr7.2:4666914-4666933 None:intergenic 55.0%
TGGGAGGTAGGAGAAGAAGG+AGG - chr7.2:4667068-4667087 MS.gene28623:intron 55.0%
GCCACCAAGACGGAGGAGTT+TGG - chr7.2:4666970-4666989 MS.gene28623:intron 60.0%
TACCGGCAACGCCACCAAGA+CGG - chr7.2:4666960-4666979 MS.gene28623:intron 60.0%
!! CTCCGTCTTGGTGGCGTTGC+CGG + chr7.2:4666965-4666984 None:intergenic 65.0%
CGGCAACGCCACCAAGACGG+AGG - chr7.2:4666963-4666982 MS.gene28623:intron 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 4666809 4667718 4666809 ID=MS.gene28623
chr7.2 mRNA 4666809 4667718 4666809 ID=MS.gene28623.t1;Parent=MS.gene28623
chr7.2 exon 4667109 4667718 4667109 ID=MS.gene28623.t1.exon1;Parent=MS.gene28623.t1
chr7.2 CDS 4667109 4667718 4667109 ID=cds.MS.gene28623.t1;Parent=MS.gene28623.t1
chr7.2 exon 4666809 4666894 4666809 ID=MS.gene28623.t1.exon2;Parent=MS.gene28623.t1
chr7.2 CDS 4666809 4666894 4666809 ID=cds.MS.gene28623.t1;Parent=MS.gene28623.t1
Gene Sequence

>MS.gene28623

ATGTGTATGTCAAACTTGTCCATTGAAAGTTTTGAAGCTGATGGTGGTGATGAAGCAGATGGTGAATTTTCTGCTGATGGAAAAGATGATCAGTTATTACAACCAGGGTTGTCTTCTGGCTCTGAAAATGAATCAGGTAGTTGTTATTCGTTACCGGCAACGCCACCAAGACGGAGGAGTTTGGTTCCGGTGAGTGTTGTTGGAGTGAAAGATTATGCAAGTGAGAATGAAGCAAGAAAAGAAACAAAGGAAAGTGGTTTGGGAGGTAGGAGAAGAAGGAGGAGAAGGAGAATGAGAAGTGTGTTTGAAAGGGGAAATAGTTGGGAGAATTTATGGGATCAGAAGAAGAAAATGATGAAAGAGAAAGAGAAATTAGAGAATGGTGTGAATAGTGGAGAGAGTGATCAGAGTACTGGGGTGATGGTGATTACTAGGCCTAAAGGTGGGAATAGGAGTTTGTGTATGGATTTGGAAGAAGTTAAAGCTTGTAGAGATCTTGGATTTGAGTTGGAACATGAAAGAATTTCTGCTGTTTCTTTCTCTAATTCAACACTTGATACTAGTAGTGGTGGTAATTCACCTATTGCTAATTGGCGAATCTCCGGTCCCGGTAATTCTCAATTTTGGTTCATATTTCCGATTAGGTCAACTTATTTGAGTTTATTTACTGCATAAACATTATAAGATTGTTTGGCAGAGTTTATGAAAACAATTTATGACATGTTCGGAAGACTCATAAGTTATTTCCATAATATATGATACGCGCTTAAGTTATAAGCCTTCGATTAATTCAAAATTTGACATTTTTGTATTATATTATGTAGGTGATGATCCAAGAGATGTGAAGGCACGACTCAAAGTGTGGGCACAGGCAGTAGCAATAGCATCTGCCACCAAATACGGCTCATGA

Protein sequence

>MS.gene28623.t1

MCMSNLSIESFEADGGDEADGEFSADGKDDQLLQPGLSSGSENESGSCYSLPATPPRRRSLVPVSVVGVKDYASENEARKETKESGLGGRRRRRRRRMRSVFERGNSWENLWDQKKKMMKEKEKLENGVNSGESDQSTGVMVITRPKGGNRSLCMDLEEVKACRDLGFELEHERISAVSFSNSTLDTSSGGNSPIANWRISGPGDDPRDVKARLKVWAQAVAIASATKYGS