AlfalfaGEDB | MS.gene30904

M. sativa cultivar XinJiangDaYe / MS.gene30904

KEGG: K02973
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015935 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00164.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30904.t1	RYR71178.1	100	136	0	0	1	136	8	143	2.00E-69	271.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30904.t1	P46297	99.3	136	1	0	1	136	7	142	1.1e-71	270.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30904.t1	A0A1S3TNG0	100.0	136	0	0	1	136	7	142	1.5e-69	271.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene30904	0.831981	1.23E-55	-1.69E-46
MS.gene050320	MS.gene30904	0.836545	8.79E-57	-1.69E-46
MS.gene050838	MS.gene30904	0.829648	4.58E-55	-1.69E-46
MS.gene050916	MS.gene30904	0.801745	7.79E-49	-1.69E-46
MS.gene051063	MS.gene30904	0.821369	4.19E-53	-1.69E-46
MS.gene051330	MS.gene30904	0.864443	1.21E-64	-1.69E-46
MS.gene051687	MS.gene30904	0.82691	2.09E-54	-1.69E-46
MS.gene051746	MS.gene30904	0.852	6.15E-61	-1.69E-46
MS.gene051748	MS.gene30904	0.862097	6.45E-64	-1.69E-46
MS.gene051756	MS.gene30904	0.804924	1.71E-49	-1.69E-46
MS.gene051850	MS.gene30904	0.831606	1.52E-55	-1.69E-46
MS.gene051903	MS.gene30904	0.91069	1.41E-82	-1.69E-46
MS.gene052202	MS.gene30904	0.802266	6.09E-49	-1.69E-46
MS.gene052818	MS.gene30904	-0.801935	7.12E-49	-1.69E-46
MS.gene052947	MS.gene30904	0.859772	3.28E-63	-1.69E-46
MS.gene053109	MS.gene30904	0.818209	2.21E-52	-1.69E-46
MS.gene053952	MS.gene30904	0.813196	2.89E-51	-1.69E-46
MS.gene054937	MS.gene30904	0.809819	1.57E-50	-1.69E-46
MS.gene055424	MS.gene30904	0.828862	7.10E-55	-1.69E-46
MS.gene056075	MS.gene30904	0.871583	6.12E-67	-1.69E-46
MS.gene056091	MS.gene30904	0.81635	5.79E-52	-1.69E-46
MS.gene056631	MS.gene30904	0.82765	1.39E-54	-1.69E-46
MS.gene057094	MS.gene30904	0.873878	1.04E-67	-1.69E-46
MS.gene057234	MS.gene30904	0.814968	1.18E-51	-1.69E-46
MS.gene057705	MS.gene30904	0.801664	8.09E-49	-1.69E-46
MS.gene058074	MS.gene30904	0.819607	1.06E-52	-1.69E-46
MS.gene058238	MS.gene30904	0.837438	5.20E-57	-1.69E-46
MS.gene058354	MS.gene30904	0.804246	2.37E-49	-1.69E-46
MS.gene059227	MS.gene30904	0.822093	2.85E-53	-1.69E-46
MS.gene059726	MS.gene30904	0.804259	2.35E-49	-1.69E-46
MS.gene06001	MS.gene30904	0.898957	3.19E-77	-1.69E-46
MS.gene061145	MS.gene30904	0.809886	1.52E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30904.t1	MTR_1g066860	100.000	136	0	0	1	136	1	136	1.11e-96	274
MS.gene30904.t1	MTR_1g066860	100.000	136	0	0	1	136	7	142	1.16e-96	274
MS.gene30904.t1	MTR_7g100720	94.118	136	8	0	1	136	7	142	1.59e-90	259
MS.gene30904.t1	MTR_7g061155	96.610	118	4	0	19	136	138	255	3.89e-80	236
MS.gene30904.t1	MTR_7g063460	60.000	55	20	1	48	102	28	80	1.78e-15	67.0
MS.gene30904.t1	MTR_8g447270	64.151	53	17	1	48	100	17	67	1.68e-14	65.5
MS.gene30904.t1	MTR_4g038400	67.347	49	13	1	50	98	103	148	1.81e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30904.t1	AT5G02960	95.588	136	6	0	1	136	7	142	6.49e-91	259
MS.gene30904.t1	AT3G09680	93.382	136	9	0	1	136	7	142	1.07e-87	251

Find 37 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACACTTACGAATAGCTGAAT+TGG	0.338181	1.2:-50292567	None:intergenic
TTCAAGGTTGTGAAGGTCTC+TGG	0.340450	1.2:+50292893	MS.gene30904:CDS
TCTCCTTCTTCTCCTTGAAA+AGG	0.352760	1.2:-50292931	None:intergenic
ATTCCACAAATCTAAGATCT+TGG	0.379899	1.2:-50292956	None:intergenic
CATGCTGTGGGAGATATTCC+TGG	0.414411	1.2:+50292863	MS.gene30904:CDS
CAGCTATTCGTAAGTGTGCC+AGG	0.416301	1.2:+50292573	MS.gene30904:CDS
CTCCTTCTTCTCCTTGAAAA+GGG	0.418297	1.2:-50292930	None:intergenic
TCATACAAGAAGTCTCATCT+TGG	0.473075	1.2:+50292370	MS.gene30904:CDS
CACTTACGAATAGCTGAATT+GGG	0.473089	1.2:-50292566	None:intergenic
TCAAGGTTGTGAAGGTCTCT+GGG	0.478339	1.2:+50292894	MS.gene30904:CDS
TGCTGGTTCTTCCCACGCAA+AGG	0.487634	1.2:+50292414	MS.gene30904:CDS
CTCACCGCAGGAGGCAAAGG+TGG	0.491520	1.2:+50292338	MS.gene30904:CDS
GCTGGTTCTTCCCACGCAAA+GGG	0.497054	1.2:+50292415	MS.gene30904:CDS
ATTGCTGGATTTGGAAGAAA+GGG	0.499669	1.2:+50292839	MS.gene30904:CDS
GCGTGGGAAGAACCAGCAAA+AGG	0.504542	1.2:-50292409	None:intergenic
CATACAAGAAGTCTCATCTT+GGG	0.508352	1.2:+50292371	MS.gene30904:CDS
GATTGCTGGATTTGGAAGAA+AGG	0.508603	1.2:+50292838	MS.gene30904:CDS
TAGTTTAAACAACCATCGTT+AGG	0.508606	1.2:-50292638	None:intergenic
TATTCCTGGAGTTAGATTCA+AGG	0.508883	1.2:+50292877	MS.gene30904:CDS
AGGGTACAACTAATCAAGAA+TGG	0.513747	1.2:+50292593	MS.gene30904:CDS
CAGCCCACCTTTGCCTCCTG+CGG	0.522174	1.2:-50292342	None:intergenic
CGAGGACAATTCCCTTTGCG+TGG	0.534957	1.2:-50292426	None:intergenic
GCAAGCTGAAGTCTCACCGC+AGG	0.557712	1.2:+50292326	MS.gene30904:CDS
AGTCTCACCGCAGGAGGCAA+AGG	0.562641	1.2:+50292335	MS.gene30904:CDS
AGCTGAAGTCTCACCGCAGG+AGG	0.569090	1.2:+50292329	MS.gene30904:CDS
AGTCTCATCTTGGGAATGAG+TGG	0.571456	1.2:+50292380	MS.gene30904:CDS
ATTCTTGATTAGTTGTACCC+TGG	0.574592	1.2:-50292591	None:intergenic
TGGAAGAAAGGGTCATGCTG+TGG	0.584767	1.2:+50292850	MS.gene30904:CDS
GGAAGAAAGGGTCATGCTGT+GGG	0.588458	1.2:+50292851	MS.gene30904:CDS
GCTGCCTTTGTTCCTAACGA+TGG	0.589634	1.2:+50292626	MS.gene30904:CDS
AGTTAGATTCAAGGTTGTGA+AGG	0.593952	1.2:+50292886	MS.gene30904:CDS
ACAACCATCGTTAGGAACAA+AGG	0.594668	1.2:-50292630	None:intergenic
TCACCGCAGGAGGCAAAGGT+GGG	0.600190	1.2:+50292339	MS.gene30904:CDS
ACAACCTTGAATCTAACTCC+AGG	0.602701	1.2:-50292881	None:intergenic
GAGGACAATTCCCTTTGCGT+GGG	0.632164	1.2:-50292425	None:intergenic
AGCTATTCGTAAGTGTGCCA+GGG	0.637371	1.2:+50292574	MS.gene30904:CDS
CAAGGTTGTGAAGGTCTCTG+GGG	0.680000	1.2:+50292895	MS.gene30904:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCATTGCTTGTATTTTCTAT+TGG	+	chr1.2:50292679-50292698	MS.gene30904:intron	25.0%
!!!	GATTGTTGTTTTATTTACAG+TGG	+	chr1.2:50292527-50292546	MS.gene30904:intron	25.0%
!!!	TTTTATTTACAGTGGTATTG+AGG	+	chr1.2:50292535-50292554	MS.gene30904:intron	25.0%
	TAGTTTAAACAACCATCGTT+AGG	-	chr1.2:50292641-50292660	None:intergenic	30.0%
	ACACTTACGAATAGCTGAAT+TGG	-	chr1.2:50292570-50292589	None:intergenic	35.0%
	AGGGTACAACTAATCAAGAA+TGG	+	chr1.2:50292593-50292612	MS.gene30904:CDS	35.0%
	AGTTAGATTCAAGGTTGTGA+AGG	+	chr1.2:50292886-50292905	MS.gene30904:CDS	35.0%
	ATTGCTGGATTTGGAAGAAA+GGG	+	chr1.2:50292839-50292858	MS.gene30904:CDS	35.0%
	CACTTACGAATAGCTGAATT+GGG	-	chr1.2:50292569-50292588	None:intergenic	35.0%
	CATACAAGAAGTCTCATCTT+GGG	+	chr1.2:50292371-50292390	MS.gene30904:CDS	35.0%
	TATTCCTGGAGTTAGATTCA+AGG	+	chr1.2:50292877-50292896	MS.gene30904:CDS	35.0%
	TCATACAAGAAGTCTCATCT+TGG	+	chr1.2:50292370-50292389	MS.gene30904:CDS	35.0%
	TGACTTTAAACTGTTGTGTC+TGG	+	chr1.2:50292711-50292730	MS.gene30904:intron	35.0%
!	ATTCTTGATTAGTTGTACCC+TGG	-	chr1.2:50292594-50292613	None:intergenic	35.0%
!!!	GAAGTTTTGATTGCTGGATT+TGG	+	chr1.2:50292830-50292849	MS.gene30904:CDS	35.0%
	ACAACCATCGTTAGGAACAA+AGG	-	chr1.2:50292633-50292652	None:intergenic	40.0%
	ACAACCTTGAATCTAACTCC+AGG	-	chr1.2:50292884-50292903	None:intergenic	40.0%
	CTCCTTCTTCTCCTTGAAAA+GGG	-	chr1.2:50292933-50292952	None:intergenic	40.0%
	GATTGCTGGATTTGGAAGAA+AGG	+	chr1.2:50292838-50292857	MS.gene30904:CDS	40.0%
	TCCTCGAAAAGATGTAAGAG+TGG	+	chr1.2:50292443-50292462	MS.gene30904:intron	40.0%
	TCTCCTTCTTCTCCTTGAAA+AGG	-	chr1.2:50292934-50292953	None:intergenic	40.0%
!	ACCACTCTTACATCTTTTCG+AGG	-	chr1.2:50292447-50292466	None:intergenic	40.0%
!!!	CAGGATGAAGTTTTGATTGC+TGG	+	chr1.2:50292824-50292843	MS.gene30904:intron	40.0%
	ACACACAGTGTCACACAAGA+TGG	-	chr1.2:50292792-50292811	None:intergenic	45.0%
	AGCTATTCGTAAGTGTGCCA+GGG	+	chr1.2:50292574-50292593	MS.gene30904:CDS	45.0%
	AGTCTCATCTTGGGAATGAG+TGG	+	chr1.2:50292380-50292399	MS.gene30904:CDS	45.0%
	TCAAGGTTGTGAAGGTCTCT+GGG	+	chr1.2:50292894-50292913	MS.gene30904:CDS	45.0%
	TTCAAGGTTGTGAAGGTCTC+TGG	+	chr1.2:50292893-50292912	MS.gene30904:CDS	45.0%
!	GAGTGGAAGAAACCTTTTGC+TGG	+	chr1.2:50292397-50292416	MS.gene30904:CDS	45.0%
!	GTCTCTTCTTGCCCTTTTCA+AGG	+	chr1.2:50292919-50292938	MS.gene30904:CDS	45.0%
!!	TGCCCTTTTCAAGGAGAAGA+AGG	+	chr1.2:50292928-50292947	MS.gene30904:CDS	45.0%
	CAAGGTTGTGAAGGTCTCTG+GGG	+	chr1.2:50292895-50292914	MS.gene30904:CDS	50.0%
	CAGCTATTCGTAAGTGTGCC+AGG	+	chr1.2:50292573-50292592	MS.gene30904:CDS	50.0%
	CATGCTGTGGGAGATATTCC+TGG	+	chr1.2:50292863-50292882	MS.gene30904:CDS	50.0%
	GAGGACAATTCCCTTTGCGT+GGG	-	chr1.2:50292428-50292447	None:intergenic	50.0%
	GCTGCCTTTGTTCCTAACGA+TGG	+	chr1.2:50292626-50292645	MS.gene30904:CDS	50.0%
	GGAAGAAAGGGTCATGCTGT+GGG	+	chr1.2:50292851-50292870	MS.gene30904:CDS	50.0%
	GTGACACTGTGTGTGACTGA+TGG	+	chr1.2:50292798-50292817	MS.gene30904:intron	50.0%
	TGGAAGAAAGGGTCATGCTG+TGG	+	chr1.2:50292850-50292869	MS.gene30904:CDS	50.0%
	TGTGTGTGACTGATGGTTGC+AGG	+	chr1.2:50292805-50292824	MS.gene30904:intron	50.0%
	CGAGGACAATTCCCTTTGCG+TGG	-	chr1.2:50292429-50292448	None:intergenic	55.0%
	GCGTGGGAAGAACCAGCAAA+AGG	-	chr1.2:50292412-50292431	None:intergenic	55.0%
!	GCTGGTTCTTCCCACGCAAA+GGG	+	chr1.2:50292415-50292434	MS.gene30904:CDS	55.0%
!!	TGCTGGTTCTTCCCACGCAA+AGG	+	chr1.2:50292414-50292433	MS.gene30904:CDS	55.0%
	AGCTGAAGTCTCACCGCAGG+AGG	+	chr1.2:50292329-50292348	MS.gene30904:CDS	60.0%
	AGTCTCACCGCAGGAGGCAA+AGG	+	chr1.2:50292335-50292354	MS.gene30904:CDS	60.0%
	GCAAGCTGAAGTCTCACCGC+AGG	+	chr1.2:50292326-50292345	MS.gene30904:CDS	60.0%
	TCACCGCAGGAGGCAAAGGT+GGG	+	chr1.2:50292339-50292358	MS.gene30904:CDS	60.0%
	CAGCCCACCTTTGCCTCCTG+CGG	-	chr1.2:50292345-50292364	None:intergenic	65.0%
	CTCACCGCAGGAGGCAAAGG+TGG	+	chr1.2:50292338-50292357	MS.gene30904:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	50292313	50292967	50292313	ID=MS.gene30904
chr1.2	mRNA	50292313	50292967	50292313	ID=MS.gene30904.t1;Parent=MS.gene30904
chr1.2	exon	50292313	50292455	50292313	ID=MS.gene30904.t1.exon1;Parent=MS.gene30904.t1
chr1.2	CDS	50292313	50292455	50292313	ID=cds.MS.gene30904.t1;Parent=MS.gene30904.t1
chr1.2	exon	50292547	50292673	50292547	ID=MS.gene30904.t1.exon2;Parent=MS.gene30904.t1
chr1.2	CDS	50292547	50292673	50292547	ID=cds.MS.gene30904.t1;Parent=MS.gene30904.t1
chr1.2	exon	50292827	50292967	50292827	ID=MS.gene30904.t1.exon3;Parent=MS.gene30904.t1
chr1.2	CDS	50292827	50292967	50292827	ID=cds.MS.gene30904.t1;Parent=MS.gene30904.t1

Gene Sequence

>MS.gene30904

ATGGGAGCTGCTCGCAAGCTGAAGTCTCACCGCAGGAGGCAAAGGTGGGCTGACAAGTCATACAAGAAGTCTCATCTTGGGAATGAGTGGAAGAAACCTTTTGCTGGTTCTTCCCACGCAAAGGGAATTGTCCTCGAAAAGATGTAAGAGTGGTTAAGCTTGCTATTTTTTTTACTAGAATTTTATTTGATATGAACATGTGATGCTAAGAAATGATTGTTGTTTTATTTACAGTGGTATTGAGGCTAAACAGCCCAATTCAGCTATTCGTAAGTGTGCCAGGGTACAACTAATCAAGAATGGAAAGAAAATTGCTGCCTTTGTTCCTAACGATGGTTGTTTAAACTACATCGAAGAAAATGTAAGTCATTGCTTGTATTTTCTATTGGTCTGATCAGTGACTTTAAACTGTTGTGTCTGGAACTGTCTTCTCTTAAATTGTTTTTCATATCTTATTAGTAATTTATTGTTTGTATCCATCTTGTGTGACACTGTGTGTGACTGATGGTTGCAGGATGAAGTTTTGATTGCTGGATTTGGAAGAAAGGGTCATGCTGTGGGAGATATTCCTGGAGTTAGATTCAAGGTTGTGAAGGTCTCTGGGGTGTCTCTTCTTGCCCTTTTCAAGGAGAAGAAGGAGAAGCCAAGATCTTAG

Protein sequence

>MS.gene30904.t1

MGAARKLKSHRRRQRWADKSYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQPNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS