AlfalfaGEDB | MS.gene34287

M. sativa cultivar XinJiangDaYe / MS.gene34287

KEGG: K00058
GO: GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0016614 GO:0016616 GO:0036094 GO:0048037 GO:0050662 GO:0051287 GO:0055114 GO:0097159 GO:1901265 GO:1901363
PFAM: PF00389.33

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34287.t1	XP_003604140.1	88.4	216	15	3	1	210	1	212	8.80E-88	333.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34287.t1	O04130	59.7	206	70	4	16	210	3	206	6.7e-52	205.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34287.t1	G7JVK0	88.4	216	15	3	1	210	1	212	6.4e-88	333.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene34287	0.821261	4.44E-53	-1.69E-46
MS.gene049711	MS.gene34287	0.83543	1.69E-56	-1.69E-46
MS.gene049744	MS.gene34287	0.812614	3.88E-51	-1.69E-46
MS.gene049824	MS.gene34287	0.806989	6.29E-50	-1.69E-46
MS.gene050221	MS.gene34287	0.805588	1.24E-49	-1.69E-46
MS.gene050559	MS.gene34287	0.840623	7.78E-58	-1.69E-46
MS.gene050561	MS.gene34287	0.843866	1.08E-58	-1.69E-46
MS.gene050562	MS.gene34287	0.848818	4.81E-60	-1.69E-46
MS.gene050657	MS.gene34287	0.812544	4.02E-51	-1.69E-46
MS.gene051021	MS.gene34287	0.812575	3.96E-51	-1.69E-46
MS.gene051247	MS.gene34287	0.80725	5.54E-50	-1.69E-46
MS.gene051279	MS.gene34287	0.86821	7.73E-66	-1.69E-46
MS.gene051332	MS.gene34287	0.803078	4.14E-49	-1.69E-46
MS.gene051720	MS.gene34287	0.805223	1.48E-49	-1.69E-46
MS.gene051744	MS.gene34287	0.871545	6.30E-67	-1.69E-46
MS.gene051859	MS.gene34287	0.800528	1.38E-48	-1.69E-46
MS.gene052037	MS.gene34287	0.842025	3.33E-58	-1.69E-46
MS.gene052239	MS.gene34287	-0.823029	1.73E-53	-1.69E-46
MS.gene052311	MS.gene34287	0.822178	2.72E-53	-1.69E-46
MS.gene052488	MS.gene34287	0.804339	2.26E-49	-1.69E-46
MS.gene052582	MS.gene34287	0.822239	2.64E-53	-1.69E-46
MS.gene052584	MS.gene34287	0.821224	4.52E-53	-1.69E-46
MS.gene052679	MS.gene34287	0.821885	3.18E-53	-1.69E-46
MS.gene052680	MS.gene34287	0.813602	2.36E-51	-1.69E-46
MS.gene052681	MS.gene34287	0.809302	2.02E-50	-1.69E-46
MS.gene052682	MS.gene34287	0.80311	4.08E-49	-1.69E-46
MS.gene053144	MS.gene34287	0.805906	1.06E-49	-1.69E-46
MS.gene053151	MS.gene34287	0.812853	3.44E-51	-1.69E-46
MS.gene053226	MS.gene34287	0.824303	8.68E-54	-1.69E-46
MS.gene053621	MS.gene34287	0.816015	6.87E-52	-1.69E-46
MS.gene054576	MS.gene34287	0.87589	2.16E-68	-1.69E-46
MS.gene054584	MS.gene34287	0.820322	7.29E-53	-1.69E-46
MS.gene054651	MS.gene34287	0.809086	2.25E-50	-1.69E-46
MS.gene056050	MS.gene34287	0.846204	2.52E-59	-1.69E-46
MS.gene056507	MS.gene34287	0.835072	2.08E-56	-1.69E-46
MS.gene05686	MS.gene34287	0.82162	3.67E-53	-1.69E-46
MS.gene057012	MS.gene34287	0.80246	5.55E-49	-1.69E-46
MS.gene057869	MS.gene34287	0.80198	6.97E-49	-1.69E-46
MS.gene058010	MS.gene34287	0.82216	2.75E-53	-1.69E-46
MS.gene058289	MS.gene34287	0.841715	4.02E-58	-1.69E-46
MS.gene058625	MS.gene34287	0.801737	7.82E-49	-1.69E-46
MS.gene058649	MS.gene34287	0.80337	3.60E-49	-1.69E-46
MS.gene058668	MS.gene34287	0.824596	7.41E-54	-1.69E-46
MS.gene059272	MS.gene34287	0.803973	2.70E-49	-1.69E-46
MS.gene059439	MS.gene34287	0.820433	6.88E-53	-1.69E-46
MS.gene059570	MS.gene34287	0.804932	1.70E-49	-1.69E-46
MS.gene059625	MS.gene34287	0.812097	5.03E-51	-1.69E-46
MS.gene059774	MS.gene34287	0.803271	3.77E-49	-1.69E-46
MS.gene060167	MS.gene34287	0.817363	3.43E-52	-1.69E-46
MS.gene060373	MS.gene34287	0.804358	2.25E-49	-1.69E-46
MS.gene060697	MS.gene34287	0.801477	8.84E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34287.t1	MTR_4g005880	89.815	216	12	3	1	210	1	212	1.52e-127	373
MS.gene34287.t1	MTR_1g107480	74.074	135	35	0	76	210	45	179	2.91e-64	209

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34287.t1	AT1G17745	59.223	206	71	4	16	210	3	206	1.72e-70	226
MS.gene34287.t1	AT1G17745	59.223	206	71	4	16	210	3	206	3.02e-70	226
MS.gene34287.t1	AT3G19480	76.800	125	29	0	86	210	46	170	2.80e-63	206
MS.gene34287.t1	AT4G34200	72.593	135	37	0	76	210	51	185	2.06e-62	204

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACAAGCTGCTACTGAATT+TGG	0.182846	4.2:-93420567	MS.gene34287:CDS
TTCCTTAACTCCAACACTTT+TGG	0.245786	4.2:-93420972	MS.gene34287:CDS
CATGTACCATACCGGCTTTA+AGG	0.285448	4.2:+93420439	None:intergenic
ATCTTCTTGCATAGGTCTTC+TGG	0.285848	4.2:+93420711	None:intergenic
ATGAGAGAGTTTAATGTTGT+TGG	0.329026	4.2:+93420941	None:intergenic
TTCCAAAAGTGTTGGAGTTA+AGG	0.352126	4.2:+93420970	None:intergenic
GTTAAGGAATGAGAGATTAG+AGG	0.361871	4.2:+93420986	None:intergenic
GCCTGCTTCTCCGAGTTTCT+CGG	0.380012	4.2:+93420785	None:intergenic
ATGTACCATACCGGCTTTAA+GGG	0.400835	4.2:+93420440	None:intergenic
AAAGGAAAGTTGAAAGTTGT+TGG	0.405100	4.2:-93420621	MS.gene34287:CDS
TTGGTTTCAGAAGAGAAGAT+AGG	0.433144	4.2:+93421020	None:intergenic
GATTGCTCTTCTTGCTGCTA+TGG	0.439120	4.2:-93420490	MS.gene34287:CDS
AACAACAGAAACAACGTTGT+TGG	0.441735	4.2:+93420842	None:intergenic
TCCGAGAAACTCGGAGAAGC+AGG	0.463753	4.2:-93420786	MS.gene34287:CDS
TATGGCTCGTAATGTTTCTC+AGG	0.473207	4.2:-93420472	MS.gene34287:CDS
TTGAAAGTTGTTGGTAGAGC+TGG	0.503758	4.2:-93420612	MS.gene34287:CDS
ACAAGACAAGTGTTTGAAGC+AGG	0.504544	4.2:-93420645	MS.gene34287:CDS
AATTGAGTTGGTACAAGAAG+AGG	0.507359	4.2:+93421052	None:intergenic
ACTATTGCTGCTGCTGAACA+TGG	0.507722	4.2:-93420513	MS.gene34287:CDS
TTTAAGACAATTAGGACATG+TGG	0.518255	4.2:-93420754	MS.gene34287:CDS
TTGTTGGTAGAGCTGGTGTT+GGG	0.537332	4.2:-93420605	MS.gene34287:CDS
TCAGAAGAGAAGATAGGTGA+AGG	0.543290	4.2:+93421026	None:intergenic
TTCTCGGAGACCAAGATCGT+TGG	0.552120	4.2:+93420801	None:intergenic
GTTGTTGGTAGAGCTGGTGT+TGG	0.558071	4.2:-93420606	MS.gene34287:CDS
ATCTTGGTCTCCGAGAAACT+CGG	0.570682	4.2:-93420795	MS.gene34287:CDS
GGTAATGATTCCAAAAGTGT+TGG	0.574616	4.2:+93420962	None:intergenic
AGCTTGAGATCTTCTTGCAT+AGG	0.577862	4.2:+93420703	None:intergenic
CAAGTGTTTGAAGCAGGGAA+AGG	0.581477	4.2:-93420639	MS.gene34287:CDS
AGGTGAAGGAAGAATTGAGT+TGG	0.583715	4.2:+93421040	None:intergenic
GCTGATGCTTCCCTTAAAGC+CGG	0.593057	4.2:-93420450	None:intergenic
GCAGCAATAGTATTAGCAGT+AGG	0.600020	4.2:+93420525	None:intergenic
CTATTGCTGCTGCTGAACAT+GGG	0.618691	4.2:-93420512	MS.gene34287:CDS
CAAGACAAGTGTTTGAAGCA+GGG	0.623927	4.2:-93420644	MS.gene34287:CDS
TGGTGTTGGGATTGATAATG+TGG	0.634025	4.2:-93420592	MS.gene34287:CDS
GATGCTTTGATTGTGAGAAG+CGG	0.715751	4.2:-93420678	MS.gene34287:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTACAAGTTTTAAGACAATT+AGG	-	chr4.2:93420748-93420767	MS.gene34287:CDS	20.0%
!!!	ATTAGAGGATTTTGAAGATT+TGG	+	chr4.2:93420512-93420531	None:intergenic	25.0%
!!!	TTTGAGTTGTTTGTTTTGTT+TGG	+	chr4.2:93420648-93420667	None:intergenic	25.0%
	AAAGGAAAGTTGAAAGTTGT+TGG	-	chr4.2:93420889-93420908	MS.gene34287:CDS	30.0%
	AAATGAAAAACCAACGATCT+TGG	-	chr4.2:93420699-93420718	MS.gene34287:CDS	30.0%
	ATGAGAGAGTTTAATGTTGT+TGG	+	chr4.2:93420572-93420591	None:intergenic	30.0%
!	TTTAAGACAATTAGGACATG+TGG	-	chr4.2:93420756-93420775	MS.gene34287:CDS	30.0%
!!!	TGTTTGTTTTGTTTGGTCAA+CGG	+	chr4.2:93420641-93420660	None:intergenic	30.0%
	AACAACAGAAACAACGTTGT+TGG	+	chr4.2:93420671-93420690	None:intergenic	35.0%
	GTTAAGGAATGAGAGATTAG+AGG	+	chr4.2:93420527-93420546	None:intergenic	35.0%
	TTGGTTTCAGAAGAGAAGAT+AGG	+	chr4.2:93420493-93420512	None:intergenic	35.0%
!	GGTAATGATTCCAAAAGTGT+TGG	+	chr4.2:93420551-93420570	None:intergenic	35.0%
!	TTCCTTAACTCCAACACTTT+TGG	-	chr4.2:93420538-93420557	MS.gene34287:CDS	35.0%
!!	AATTGAGTTGGTACAAGAAG+AGG	+	chr4.2:93420461-93420480	None:intergenic	35.0%
!!	TTCCAAAAGTGTTGGAGTTA+AGG	+	chr4.2:93420543-93420562	None:intergenic	35.0%
	AGCTTGAGATCTTCTTGCAT+AGG	+	chr4.2:93420810-93420829	None:intergenic	40.0%
	AGGTGAAGGAAGAATTGAGT+TGG	+	chr4.2:93420473-93420492	None:intergenic	40.0%
	ATCTTCTTGCATAGGTCTTC+TGG	+	chr4.2:93420802-93420821	None:intergenic	40.0%
	CTACAAGCTGCTACTGAATT+TGG	-	chr4.2:93420943-93420962	MS.gene34287:CDS	40.0%
	GATGCTTTGATTGTGAGAAG+CGG	-	chr4.2:93420832-93420851	MS.gene34287:CDS	40.0%
	GCAGCAATAGTATTAGCAGT+AGG	+	chr4.2:93420988-93421007	None:intergenic	40.0%
	GTACAAGAAGAGGAAGACAT+CGG	+	chr4.2:93420451-93420470	None:intergenic	40.0%
	TATGGCTCGTAATGTTTCTC+AGG	-	chr4.2:93421038-93421057	MS.gene34287:CDS	40.0%
	TCAGAAGAGAAGATAGGTGA+AGG	+	chr4.2:93420487-93420506	None:intergenic	40.0%
!	ACAAGACAAGTGTTTGAAGC+AGG	-	chr4.2:93420865-93420884	MS.gene34287:CDS	40.0%
!	CAAGACAAGTGTTTGAAGCA+GGG	-	chr4.2:93420866-93420885	MS.gene34287:CDS	40.0%
!	TTGAAAGTTGTTGGTAGAGC+TGG	-	chr4.2:93420898-93420917	MS.gene34287:CDS	40.0%
!!	CTCTCAACAACGTTGTTTTG+TGG	-	chr4.2:93420597-93420616	MS.gene34287:CDS	40.0%
!!	TGGTGTTGGGATTGATAATG+TGG	-	chr4.2:93420918-93420937	MS.gene34287:CDS	40.0%
	ACTATTGCTGCTGCTGAACA+TGG	-	chr4.2:93420997-93421016	MS.gene34287:CDS	45.0%
	ATCTTGGTCTCCGAGAAACT+CGG	-	chr4.2:93420715-93420734	MS.gene34287:CDS	45.0%
	CTATTGCTGCTGCTGAACAT+GGG	-	chr4.2:93420998-93421017	MS.gene34287:CDS	45.0%
	GATTGCTCTTCTTGCTGCTA+TGG	-	chr4.2:93421020-93421039	MS.gene34287:CDS	45.0%
!	CAAGTGTTTGAAGCAGGGAA+AGG	-	chr4.2:93420871-93420890	MS.gene34287:CDS	45.0%
!	TTGTTGGTAGAGCTGGTGTT+GGG	-	chr4.2:93420905-93420924	MS.gene34287:CDS	45.0%
	TTCTCGGAGACCAAGATCGT+TGG	+	chr4.2:93420712-93420731	None:intergenic	50.0%
!	GTTGTTGGTAGAGCTGGTGT+TGG	-	chr4.2:93420904-93420923	MS.gene34287:CDS	50.0%
	GCCTGCTTCTCCGAGTTTCT+CGG	+	chr4.2:93420728-93420747	None:intergenic	55.0%
	TCCGAGAAACTCGGAGAAGC+AGG	-	chr4.2:93420724-93420743	MS.gene34287:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	93420451	93421081	93420451	ID=MS.gene34287
chr4.2	mRNA	93420451	93421081	93420451	ID=MS.gene34287.t1;Parent=MS.gene34287
chr4.2	exon	93420451	93421081	93420451	ID=MS.gene34287.t1.exon1;Parent=MS.gene34287.t1
chr4.2	CDS	93420451	93421081	93420451	ID=cds.MS.gene34287.t1;Parent=MS.gene34287.t1

Gene Sequence

>MS.gene34287

ATGTCTTCCTCTTCTTGTACCAACTCAATTCTTCCTTCACCTATCTTCTCTTCTGAAACCAAATCTTCAAAATCCTCTAATCTCTCATTCCTTAACTCCAACACTTTTGGAATCATTACCAACAACATTAAACTCTCTCATGCTAACTCTCAACAACGTTGTTTTGTGGTCAACAGTGTTTTAAAAACCGTTGACCAAACAAAACAAACAACTCAAACCAACAACGTTGTTTCTGTTGTTAACGATGAAAATGAAAAACCAACGATCTTGGTCTCCGAGAAACTCGGAGAAGCAGGCTTACAAGTTTTAAGACAATTAGGACATGTGGAATGTGCTTATGATCTTTCACCAGAAGACCTATGCAAGAAGATCTCAAGCTGTGATGCTTTGATTGTGAGAAGCGGTACGAAAGTCACAAGACAAGTGTTTGAAGCAGGGAAAGGAAAGTTGAAAGTTGTTGGTAGAGCTGGTGTTGGGATTGATAATGTGGATCTACAAGCTGCTACTGAATTTGGTTGTCTTGTTGTGAATGCTCCTACTGCTAATACTATTGCTGCTGCTGAACATGGGATTGCTCTTCTTGCTGCTATGGCTCGTAATGTTTCTCAGGCTGATGCTTCCCTTAAAGCCG

Protein sequence

>MS.gene34287.t1

MSSSSCTNSILPSPIFSSETKSSKSSNLSFLNSNTFGIITNNIKLSHANSQQRCFVVNSVLKTVDQTKQTTQTNNVVSVVNDENEKPTILVSEKLGEAGLQVLRQLGHVECAYDLSPEDLCKKISSCDALIVRSGTKVTRQVFEAGKGKLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLAAMARNVSQADASLKA