AlfalfaGEDB | MS.gene34296

M. sativa cultivar XinJiangDaYe / MS.gene34296

KEGG: -
GO: GO:0005575 GO:0016020 GO:0110165
PFAM: PF02325.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34296.t1	XP_013454503.1	94.8	97	5	0	1	97	118	214	5.20E-44	186.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34296.t1	Q9SUE0	86.2	87	12	0	7	93	127	213	1.9e-39	162.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34296.t1	A0A072UFK1	94.8	97	5	0	1	97	118	214	3.7e-44	186.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene34296	0.808037	3.77E-50	-1.69E-46
MS.gene049173	MS.gene34296	0.814594	1.42E-51	-1.69E-46
MS.gene049427	MS.gene34296	0.813962	1.96E-51	-1.69E-46
MS.gene049805	MS.gene34296	0.813782	2.15E-51	-1.69E-46
MS.gene050163	MS.gene34296	0.832068	1.17E-55	-1.69E-46
MS.gene050165	MS.gene34296	0.817102	3.93E-52	-1.69E-46
MS.gene050333	MS.gene34296	0.824609	7.36E-54	-1.69E-46
MS.gene050444	MS.gene34296	0.805586	1.24E-49	-1.69E-46
MS.gene050767	MS.gene34296	0.826806	2.22E-54	-1.69E-46
MS.gene050770	MS.gene34296	0.828699	7.78E-55	-1.69E-46
MS.gene050812	MS.gene34296	0.81554	8.77E-52	-1.69E-46
MS.gene051228	MS.gene34296	0.813499	2.48E-51	-1.69E-46
MS.gene051666	MS.gene34296	0.818899	1.54E-52	-1.69E-46
MS.gene052290	MS.gene34296	0.810898	9.17E-51	-1.69E-46
MS.gene052379	MS.gene34296	0.826034	3.38E-54	-1.69E-46
MS.gene052870	MS.gene34296	0.804315	2.29E-49	-1.69E-46
MS.gene052872	MS.gene34296	0.806444	8.20E-50	-1.69E-46
MS.gene053009	MS.gene34296	0.816822	4.54E-52	-1.69E-46
MS.gene053284	MS.gene34296	0.917378	5.71E-86	-1.69E-46
MS.gene053285	MS.gene34296	0.934081	7.00E-96	-1.69E-46
MS.gene053322	MS.gene34296	0.812847	3.45E-51	-1.69E-46
MS.gene053632	MS.gene34296	0.817815	2.71E-52	-1.69E-46
MS.gene054376	MS.gene34296	0.813219	2.86E-51	-1.69E-46
MS.gene054642	MS.gene34296	0.801811	7.55E-49	-1.69E-46
MS.gene055397	MS.gene34296	0.820113	8.14E-53	-1.69E-46
MS.gene055412	MS.gene34296	0.823768	1.16E-53	-1.69E-46
MS.gene055499	MS.gene34296	0.811723	6.07E-51	-1.69E-46
MS.gene055941	MS.gene34296	0.811536	6.67E-51	-1.69E-46
MS.gene056796	MS.gene34296	0.802915	4.47E-49	-1.69E-46
MS.gene056897	MS.gene34296	0.844186	8.83E-59	-1.69E-46
MS.gene056903	MS.gene34296	0.820775	5.74E-53	-1.69E-46
MS.gene057129	MS.gene34296	0.850944	1.22E-60	-1.69E-46
MS.gene057295	MS.gene34296	0.817563	3.09E-52	-1.69E-46
MS.gene058082	MS.gene34296	0.813827	2.10E-51	-1.69E-46
MS.gene058293	MS.gene34296	0.815656	8.27E-52	-1.69E-46
MS.gene058748	MS.gene34296	0.822092	2.85E-53	-1.69E-46
MS.gene058773	MS.gene34296	0.800767	1.24E-48	-1.69E-46
MS.gene058838	MS.gene34296	0.800355	1.50E-48	-1.69E-46
MS.gene05959	MS.gene34296	0.806132	9.54E-50	-1.69E-46
MS.gene059676	MS.gene34296	0.808589	2.88E-50	-1.69E-46
MS.gene059960	MS.gene34296	0.839549	1.48E-57	-1.69E-46
MS.gene06111	MS.gene34296	0.817301	3.54E-52	-1.69E-46
MS.gene061150	MS.gene34296	0.851713	7.42E-61	-1.69E-46
MS.gene061200	MS.gene34296	0.832606	8.59E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34296.t1	MTR_4g005520	94.845	97	5	0	1	97	118	214	2.00e-62	189
MS.gene34296.t1	MTR_3g105100	47.368	76	35	3	15	89	120	191	2.82e-14	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34296.t1	AT4G27990	85.227	88	13	0	7	94	127	214	9.48e-52	162
MS.gene34296.t1	AT3G07430	83.908	87	14	0	7	93	141	227	7.07e-51	160
MS.gene34296.t1	AT5G21920	50.000	76	33	3	15	89	133	204	8.24e-15	67.8

Find 33 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACAACCATTGTCTGCAATT+AGG	0.283838	4.3:+88865705	MS.gene34296:CDS
ACAACCATTGTCTGCAATTA+GGG	0.307081	4.3:+88865706	MS.gene34296:CDS
ACAGTGGTGTGTTGTTGGTT+AGG	0.327645	4.3:+88865645	MS.gene34296:CDS
ATCCTTATCTAAGTTTGTTT+AGG	0.329465	4.3:+88865738	MS.gene34296:CDS
CAGTGGTGTGTTGTTGGTTA+GGG	0.363509	4.3:+88865646	MS.gene34296:CDS
TAGCGGCGGGGTTGAAGAAA+TGG	0.403666	4.3:+88865612	MS.gene34296:CDS
TATTTACAGTGGTGTGTTGT+TGG	0.416695	4.3:+88865640	MS.gene34296:CDS
GACGGTGAACACGCCGTTGA+CGG	0.439588	4.3:+88865586	MS.gene34296:CDS
CCGTTGACGGTTGTAGCGGC+GGG	0.441420	4.3:+88865599	MS.gene34296:CDS
TGTCTTTCCCATGGAATGTT+GGG	0.454889	4.3:-88865686	None:intergenic
CCATCCCATCCCATCCCTAC+AGG	0.465722	4.3:+88865862	MS.gene34296:CDS
CCTGTAGGGATGGGATGGGA+TGG	0.503951	4.3:-88865862	None:intergenic
GGGATGGGATGGGATCTAAC+AGG	0.504469	4.3:-88865851	None:intergenic
AAATGAAATACCTGTAGGGA+TGG	0.510722	4.3:-88865872	None:intergenic
GTTGGTTTCCCAACATTCCA+TGG	0.512936	4.3:+88865678	MS.gene34296:CDS
GACAATGGTTGTCTTTCCCA+TGG	0.515980	4.3:-88865695	None:intergenic
TTCCTAAACAAACTTAGATA+AGG	0.528049	4.3:-88865740	None:intergenic
AAATACCTGTAGGGATGGGA+TGG	0.531495	4.3:-88865867	None:intergenic
GCCGTTGACGGTTGTAGCGG+CGG	0.535668	4.3:+88865598	MS.gene34296:CDS
CTGTAGGGATGGGATGGGAT+GGG	0.538203	4.3:-88865861	None:intergenic
TTGGTTTCCCAACATTCCAT+GGG	0.548737	4.3:+88865679	MS.gene34296:CDS
CCCGCCGCTACAACCGTCAA+CGG	0.573983	4.3:-88865599	None:intergenic
TGAGAAATGAAATACCTGTA+GGG	0.574351	4.3:-88865876	None:intergenic
TTGTCTTTCCCATGGAATGT+TGG	0.574451	4.3:-88865687	None:intergenic
AAAACAGCAAAAGCAAGAAG+AGG	0.574512	4.3:-88865800	None:intergenic
CACGCCGTTGACGGTTGTAG+CGG	0.575214	4.3:+88865595	MS.gene34296:CDS
AATGAAATACCTGTAGGGAT+GGG	0.588711	4.3:-88865871	None:intergenic
CGTTGACGGTTGTAGCGGCG+GGG	0.610796	4.3:+88865600	MS.gene34296:CDS
GGCGGGGTTGAAGAAATGGT+TGG	0.623671	4.3:+88865616	MS.gene34296:CDS
AAATGGTTGGATATTTACAG+TGG	0.642279	4.3:+88865629	MS.gene34296:CDS
AATACCTGTAGGGATGGGAT+GGG	0.649146	4.3:-88865866	None:intergenic
AGATCCCTAATTGCAGACAA+TGG	0.662621	4.3:-88865710	None:intergenic
GGGATCTAACAGGAACAGTG+AGG	0.699102	4.3:-88865841	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTCCTAAACAAACTTAGATA+AGG	-	chr4.3:88865743-88865762	None:intergenic	25.0%
!!	ATCCTTATCTAAGTTTGTTT+AGG	+	chr4.3:88865738-88865757	MS.gene34296:CDS	25.0%
!!!	ATATCAAGAGTATCAAAAAC+AGG	-	chr4.3:88865776-88865795	None:intergenic	25.0%
	AAATGGTTGGATATTTACAG+TGG	+	chr4.3:88865629-88865648	MS.gene34296:CDS	30.0%
	AAAACAGCAAAAGCAAGAAG+AGG	-	chr4.3:88865803-88865822	None:intergenic	35.0%
	ACAACCATTGTCTGCAATTA+GGG	+	chr4.3:88865706-88865725	MS.gene34296:CDS	35.0%
	TATTTACAGTGGTGTGTTGT+TGG	+	chr4.3:88865640-88865659	MS.gene34296:CDS	35.0%
!!	TCAAGAGTATCAAAAACAGG+TGG	-	chr4.3:88865773-88865792	None:intergenic	35.0%
!!!	CTTCTTGCTTTTGCTGTTTT+GGG	+	chr4.3:88865803-88865822	MS.gene34296:CDS	35.0%
!!!	TCTTCTTGCTTTTGCTGTTT+TGG	+	chr4.3:88865802-88865821	MS.gene34296:CDS	35.0%
	AGATCCCTAATTGCAGACAA+TGG	-	chr4.3:88865713-88865732	None:intergenic	40.0%
	GACAACCATTGTCTGCAATT+AGG	+	chr4.3:88865705-88865724	MS.gene34296:CDS	40.0%
	TGTCTTTCCCATGGAATGTT+GGG	-	chr4.3:88865689-88865708	None:intergenic	40.0%
	TTGGTTTCCCAACATTCCAT+GGG	+	chr4.3:88865679-88865698	MS.gene34296:CDS	40.0%
	TTGTCTTTCCCATGGAATGT+TGG	-	chr4.3:88865690-88865709	None:intergenic	40.0%
!!!	TGGTTAGGGTTTTGTTGAGT+TGG	+	chr4.3:88865660-88865679	MS.gene34296:CDS	40.0%
	GACAATGGTTGTCTTTCCCA+TGG	-	chr4.3:88865698-88865717	None:intergenic	45.0%
!	ACAGTGGTGTGTTGTTGGTT+AGG	+	chr4.3:88865645-88865664	MS.gene34296:CDS	45.0%
!	CAGTGGTGTGTTGTTGGTTA+GGG	+	chr4.3:88865646-88865665	MS.gene34296:CDS	45.0%
!	GTTGGTTTCCCAACATTCCA+TGG	+	chr4.3:88865678-88865697	MS.gene34296:CDS	45.0%
	GAAAAAGACTAGGACGGGGA+CGG	+	chr4.3:88865568-88865587	MS.gene34296:CDS	50.0%
	GGGATCTAACAGGAACAGTG+AGG	-	chr4.3:88865844-88865863	None:intergenic	50.0%
!!	GCTGTTTTGGGTACTCTTGC+TGG	+	chr4.3:88865815-88865834	MS.gene34296:CDS	50.0%
	CTGTAGGGATGGGATGGGAT+GGG	-	chr4.3:88865864-88865883	None:intergenic	55.0%
	GGCGGGGTTGAAGAAATGGT+TGG	+	chr4.3:88865616-88865635	MS.gene34296:CDS	55.0%
	GGGATGGGATGGGATCTAAC+AGG	-	chr4.3:88865854-88865873	None:intergenic	55.0%
	TAGCGGCGGGGTTGAAGAAA+TGG	+	chr4.3:88865612-88865631	MS.gene34296:CDS	55.0%
	CACGCCGTTGACGGTTGTAG+CGG	+	chr4.3:88865595-88865614	MS.gene34296:CDS	60.0%
	GACGGTGAACACGCCGTTGA+CGG	+	chr4.3:88865586-88865605	MS.gene34296:CDS	60.0%
	CCCGCCGCTACAACCGTCAA+CGG	-	chr4.3:88865602-88865621	None:intergenic	65.0%
	CCGTTGACGGTTGTAGCGGC+GGG	+	chr4.3:88865599-88865618	MS.gene34296:CDS	65.0%
	CGTTGACGGTTGTAGCGGCG+GGG	+	chr4.3:88865600-88865619	MS.gene34296:CDS	65.0%
	GCCGTTGACGGTTGTAGCGG+CGG	+	chr4.3:88865598-88865617	MS.gene34296:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	88865566	88865883	88865566	ID=MS.gene34296
chr4.3	mRNA	88865566	88865883	88865566	ID=MS.gene34296.t1;Parent=MS.gene34296
chr4.3	exon	88865566	88865883	88865566	ID=MS.gene34296.t1.exon1;Parent=MS.gene34296.t1
chr4.3	CDS	88865566	88865883	88865566	ID=cds.MS.gene34296.t1;Parent=MS.gene34296.t1

Gene Sequence	>MS.gene34296 ATGAAAAAGACTAGGACGGGGACGGTGAACACGCCGTTGACGGTTGTAGCGGCGGGGTTGAAGAAATGGTTGGATATTTACAGTGGTGTGTTGTTGGTTAGGGTTTTGTTGAGTTGGTTTCCCAACATTCCATGGGAAAGACAACCATTGTCTGCAATTAGGGATCTTTGTGATCCTTATCTAAGTTTGTTTAGGAATGTTGTTCCACCTGTTTTTGATACTCTTGATATTAGTCCTCTTCTTGCTTTTGCTGTTTTGGGTACTCTTGCTGGTCTCCTCACTGTTCCTGTTAGATCCCATCCCATCCCATCCCTACAG
Protein sequence	>MS.gene34296.t1 MKKTRTGTVNTPLTVVAAGLKKWLDIYSGVLLVRVLLSWFPNIPWERQPLSAIRDLCDPYLSLFRNVVPPVFDTLDISPLLAFAVLGTLAGLLTVPVRSHPIPSLQ