AlfalfaGEDB | MS.gene35044

M. sativa cultivar XinJiangDaYe / MS.gene35044

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35044.t1	QDC33491.1	100	107	0	0	1	107	86	192	7.30E-46	193

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35044.t1	Q8GWK6	64.6	113	27	3	3	106	79	187	3.9e-24	112.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35044.t1	A0A072UPE9	91.6	107	9	0	1	107	85	191	5.2e-46	193.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene35044.t1	TF	NAC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene35044	0.819495	1.13E-52	-1.69E-46
MS.gene049254	MS.gene35044	0.849919	2.37E-60	-1.69E-46
MS.gene051247	MS.gene35044	0.80395	2.73E-49	-1.69E-46
MS.gene051332	MS.gene35044	0.988819	1.54E-175	-1.69E-46
MS.gene051574	MS.gene35044	0.817093	3.94E-52	-1.69E-46
MS.gene051744	MS.gene35044	0.815552	8.72E-52	-1.69E-46
MS.gene05191	MS.gene35044	0.806953	6.40E-50	-1.69E-46
MS.gene052333	MS.gene35044	0.804466	2.13E-49	-1.69E-46
MS.gene052463	MS.gene35044	0.803042	4.21E-49	-1.69E-46
MS.gene052519	MS.gene35044	0.800834	1.20E-48	-1.69E-46
MS.gene052534	MS.gene35044	0.822259	2.61E-53	-1.69E-46
MS.gene052582	MS.gene35044	0.850321	1.83E-60	-1.69E-46
MS.gene052584	MS.gene35044	0.840113	1.06E-57	-1.69E-46
MS.gene052773	MS.gene35044	0.814861	1.24E-51	-1.69E-46
MS.gene053226	MS.gene35044	0.818181	2.24E-52	-1.69E-46
MS.gene05415	MS.gene35044	0.812043	5.17E-51	-1.69E-46
MS.gene05423	MS.gene35044	0.81769	2.89E-52	-1.69E-46
MS.gene054479	MS.gene35044	0.817484	3.22E-52	-1.69E-46
MS.gene054576	MS.gene35044	0.81083	9.48E-51	-1.69E-46
MS.gene05458	MS.gene35044	0.806955	6.40E-50	-1.69E-46
MS.gene054651	MS.gene35044	0.824456	7.99E-54	-1.69E-46
MS.gene054991	MS.gene35044	0.808532	2.96E-50	-1.69E-46
MS.gene055228	MS.gene35044	0.804336	2.27E-49	-1.69E-46
MS.gene055586	MS.gene35044	0.820182	7.85E-53	-1.69E-46
MS.gene055724	MS.gene35044	0.804293	2.32E-49	-1.69E-46
MS.gene055818	MS.gene35044	0.800845	1.19E-48	-1.69E-46
MS.gene056050	MS.gene35044	0.825269	5.14E-54	-1.69E-46
MS.gene056162	MS.gene35044	0.82308	1.68E-53	-1.69E-46
MS.gene05659	MS.gene35044	0.804566	2.03E-49	-1.69E-46
MS.gene05686	MS.gene35044	0.820217	7.71E-53	-1.69E-46
MS.gene05690	MS.gene35044	0.801569	8.46E-49	-1.69E-46
MS.gene057026	MS.gene35044	0.806993	6.28E-50	-1.69E-46
MS.gene057208	MS.gene35044	0.84157	4.39E-58	-1.69E-46
MS.gene057611	MS.gene35044	0.825642	4.19E-54	-1.69E-46
MS.gene057855	MS.gene35044	0.807492	4.92E-50	-1.69E-46
MS.gene058979	MS.gene35044	0.822469	2.33E-53	-1.69E-46
MS.gene05897	MS.gene35044	0.814625	1.40E-51	-1.69E-46
MS.gene059438	MS.gene35044	0.809616	1.73E-50	-1.69E-46
MS.gene059439	MS.gene35044	0.826385	2.79E-54	-1.69E-46
MS.gene059440	MS.gene35044	0.807181	5.73E-50	-1.69E-46
MS.gene059625	MS.gene35044	0.802611	5.17E-49	-1.69E-46
MS.gene060365	MS.gene35044	0.804204	2.42E-49	-1.69E-46
MS.gene060716	MS.gene35044	0.800629	1.32E-48	-1.69E-46
MS.gene060777	MS.gene35044	0.820534	6.52E-53	-1.69E-46
MS.gene060935	MS.gene35044	0.806655	7.40E-50	-1.69E-46
MS.gene060937	MS.gene35044	0.815117	1.09E-51	-1.69E-46
MS.gene061188	MS.gene35044	0.831235	1.87E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35044.t1	MTR_4g094302	99.065	107	1	0	1	107	85	191	1.17e-74	219
MS.gene35044.t1	MTR_4g094302	90.667	75	4	1	1	72	85	159	4.82e-42	135
MS.gene35044.t1	MTR_3g096400	37.931	116	53	3	10	106	92	207	4.96e-20	80.9
MS.gene35044.t1	MTR_8g093790	39.496	119	44	5	4	107	80	185	1.67e-18	76.3
MS.gene35044.t1	MTR_8g059170	42.254	71	37	1	10	80	103	169	1.88e-15	70.5
MS.gene35044.t1	MTR_2g079990	42.254	71	35	2	10	80	105	169	8.22e-14	65.9
MS.gene35044.t1	MTR_8g024480	39.130	69	39	1	10	75	107	175	5.46e-13	63.5
MS.gene35044.t1	MTR_2g080010	37.662	77	43	1	4	75	100	176	5.58e-13	63.5
MS.gene35044.t1	MTR_2157s0010	40.789	76	41	2	4	75	93	168	6.03e-13	63.2
MS.gene35044.t1	MTR_3g088110	48.333	60	28	1	16	75	101	157	6.87e-13	63.2
MS.gene35044.t1	MTR_7g005280	37.143	70	36	1	4	73	97	158	1.08e-12	62.4
MS.gene35044.t1	MTR_7g100990	43.836	73	37	2	4	75	96	165	1.41e-12	62.4
MS.gene35044.t1	MTR_2g062730	35.135	74	40	1	7	80	100	165	1.93e-12	62.0
MS.gene35044.t1	MTR_6g012670	51.724	58	24	2	18	75	117	170	2.29e-12	61.6
MS.gene35044.t1	MTR_4g089135	33.708	89	42	2	4	75	100	188	2.42e-12	61.6
MS.gene35044.t1	MTR_3g070040	40.278	72	41	1	15	86	99	168	3.54e-12	61.2
MS.gene35044.t1	MTR_8g094580	43.333	60	32	1	16	75	47	104	4.23e-12	60.5
MS.gene35044.t1	MTR_8g094580	43.333	60	32	1	16	75	101	158	4.71e-12	60.8
MS.gene35044.t1	MTR_3g070030	45.902	61	30	1	15	75	113	170	5.04e-12	60.8
MS.gene35044.t1	MTR_3g109340	33.721	86	45	2	4	89	107	180	8.31e-12	60.5
MS.gene35044.t1	MTR_1g096430	39.394	66	32	1	10	75	99	156	9.97e-12	60.1
MS.gene35044.t1	MTR_3g109340	33.721	86	44	2	4	89	107	179	1.00e-11	60.1
MS.gene35044.t1	MTR_3g096920	43.333	60	31	1	16	75	101	157	1.00e-11	59.7
MS.gene35044.t1	MTR_0036s0150	39.394	66	33	2	10	75	97	155	1.57e-11	59.7
MS.gene35044.t1	MTR_2g064090	42.424	66	33	1	10	75	97	157	1.77e-11	59.3
MS.gene35044.t1	MTR_1g090723	40.000	60	30	1	16	75	106	159	3.21e-11	58.9
MS.gene35044.t1	MTR_2g093810	37.681	69	41	1	4	72	99	165	4.89e-11	58.2
MS.gene35044.t1	MTR_1g008740	37.500	72	38	1	4	75	113	177	5.42e-11	58.2
MS.gene35044.t1	MTR_1g008740	37.500	72	38	1	4	75	99	163	5.82e-11	57.8
MS.gene35044.t1	MTR_4g035590	40.000	60	27	1	16	75	106	156	6.93e-11	57.8
MS.gene35044.t1	MTR_8g467490	36.000	75	45	1	4	75	99	173	8.81e-11	57.4
MS.gene35044.t1	MTR_6g011860	42.029	69	37	1	10	75	105	173	9.05e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35044.t1	AT5G64530	60.177	113	32	3	3	106	79	187	1.25e-32	112
MS.gene35044.t1	AT3G15500	45.070	71	33	2	10	80	103	167	5.49e-14	66.2
MS.gene35044.t1	AT1G52890	43.662	71	34	2	10	80	103	167	1.52e-13	65.1
MS.gene35044.t1	AT4G27410	43.662	71	34	2	10	80	103	167	2.27e-13	64.3
MS.gene35044.t1	AT4G27410	40.845	71	36	1	10	80	120	184	2.40e-13	64.3
MS.gene35044.t1	AT2G24430	40.541	74	37	1	4	77	101	167	2.93e-13	64.3
MS.gene35044.t1	AT2G24430	40.541	74	37	1	4	77	101	167	2.93e-13	64.3
MS.gene35044.t1	AT1G77450	44.595	74	31	2	16	82	104	174	5.55e-13	63.2
MS.gene35044.t1	AT1G01720	37.234	94	52	3	16	106	101	190	6.42e-13	63.2
MS.gene35044.t1	AT5G13180	44.286	70	32	1	4	73	96	158	1.05e-12	62.4
MS.gene35044.t1	AT3G04060	39.362	94	44	4	18	106	118	203	1.42e-12	62.4
MS.gene35044.t1	AT1G69490	43.836	73	35	3	4	75	94	161	2.26e-12	61.6
MS.gene35044.t1	AT3G04070	45.161	62	33	1	15	75	109	170	2.40e-12	62.0
MS.gene35044.t1	AT1G56010	35.165	91	49	2	16	106	48	128	4.06e-12	60.8
MS.gene35044.t1	AT1G56010	38.462	78	45	1	16	93	115	189	4.99e-12	60.8
MS.gene35044.t1	AT1G76420	37.179	78	42	1	4	81	107	177	5.23e-12	60.8
MS.gene35044.t1	AT5G61430	46.552	58	27	1	18	75	50	103	7.69e-12	60.1
MS.gene35044.t1	AT5G64530	56.522	46	16	1	3	48	79	120	7.94e-12	58.5
MS.gene35044.t1	AT1G61110	37.975	79	42	3	4	76	101	178	8.57e-12	60.1
MS.gene35044.t1	AT5G61430	46.552	58	27	1	18	75	113	166	8.86e-12	60.1
MS.gene35044.t1	AT5G63790	45.000	60	32	1	16	75	144	202	9.23e-12	60.1
MS.gene35044.t1	AT5G63790	45.000	60	32	1	16	75	154	212	9.53e-12	60.1
MS.gene35044.t1	AT1G79580	41.270	63	28	1	13	75	113	166	1.02e-11	60.1
MS.gene35044.t1	AT1G79580	41.270	63	28	1	13	75	113	166	1.02e-11	60.1
MS.gene35044.t1	AT1G79580	41.270	63	28	1	13	75	113	166	1.02e-11	60.1
MS.gene35044.t1	AT1G79580	41.270	63	28	1	13	75	113	166	1.02e-11	60.1
MS.gene35044.t1	AT1G79580	41.270	63	28	1	13	75	113	166	1.02e-11	60.1
MS.gene35044.t1	AT2G46770	35.616	73	40	1	10	75	108	180	1.41e-11	59.7
MS.gene35044.t1	AT5G07680	46.552	58	27	1	18	75	114	167	1.47e-11	59.7
MS.gene35044.t1	AT5G07680	46.552	58	27	1	18	75	100	153	1.59e-11	59.3
MS.gene35044.t1	AT2G33480	42.188	64	31	1	18	81	52	109	1.77e-11	58.5
MS.gene35044.t1	AT5G18270	45.763	59	28	1	18	76	119	173	1.85e-11	59.3
MS.gene35044.t1	AT5G18270	45.763	59	28	1	18	76	119	173	1.93e-11	59.3
MS.gene35044.t1	AT5G08790	45.000	60	32	1	16	75	101	159	2.58e-11	58.5
MS.gene35044.t1	AT5G39610	35.556	90	48	3	18	107	117	196	3.02e-11	58.5
MS.gene35044.t1	AT2G33480	37.179	78	41	2	4	81	97	166	3.18e-11	58.5
MS.gene35044.t1	AT4G10350	42.623	61	29	1	13	73	104	158	3.69e-11	58.5
MS.gene35044.t1	AT4G10350	42.623	61	29	1	13	73	57	111	4.20e-11	58.2
MS.gene35044.t1	AT3G15510	30.392	102	55	3	4	94	102	198	4.20e-11	58.5
MS.gene35044.t1	AT5G04410	44.068	59	25	2	18	75	108	159	6.58e-11	57.8
MS.gene35044.t1	AT2G33480	35.897	78	40	2	4	81	97	164	9.80e-11	57.0

Find 19 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCATTTGCATCATCTAATT+TGG	0.155320	4.4:+20584799	None:intergenic
AAGAATATACTAACTGATTT+TGG	0.171613	4.4:+20585148	None:intergenic
CATTAGGTGCTTCTCCAATA+TGG	0.269862	4.4:+20585260	None:intergenic
AAATATTATGTGTTCCATAT+TGG	0.324951	4.4:-20585274	MS.gene35044:CDS
CAATTGGTTTGGTTACCATT+AGG	0.336748	4.4:+20585244	None:intergenic
TGGGACAGAGCTATCTTGTT+TGG	0.375974	4.4:-20584866	MS.gene35044:CDS
ATATTCTTGCATTATCCAAT+TGG	0.402397	4.4:+20585228	None:intergenic
CTTGCATTATCCAATTGGTT+TGG	0.405683	4.4:+20585233	None:intergenic
CTAATGGTAACCAAACCAAT+TGG	0.465135	4.4:-20585243	MS.gene35044:CDS
CAGTGGTTATTGGATGCCAA+TGG	0.509528	4.4:-20585350	None:intergenic
AGCTCAAGCAATAAGAGAGT+TGG	0.517821	4.4:-20585304	MS.gene35044:CDS
CATATTGGAGAAGCACCTAA+TGG	0.525131	4.4:-20585259	MS.gene35044:CDS
AGTGGTTATTGGATGCCAAT+GGG	0.539139	4.4:-20585349	None:intergenic
ACTTGTTCTTCCATTCCCAT+TGG	0.540129	4.4:+20585334	None:intergenic
GAAGATGATCAAGATGGAGA+TGG	0.565312	4.4:-20584886	MS.gene35044:CDS
AAGATGATCAAGATGGAGAT+GGG	0.567207	4.4:-20584885	MS.gene35044:CDS
TTATTGGATGCCAATGGGAA+TGG	0.569720	4.4:-20585344	None:intergenic
TTTGGATGATGATCTGCTAG+AGG	0.602702	4.4:+20585183	None:intergenic
CGTGATGAAGATGATCAAGA+TGG	0.626157	4.4:-20584892	MS.gene35044:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATATTATGTGTTCCATAT+TGG	-	chr4.4:20584876-20584895	MS.gene35044:CDS	20.0%
!!!	AAGAATATACTAACTGATTT+TGG	+	chr4.4:20585005-20585024	None:intergenic	20.0%
!!!	TACAGAGATATTTAAAATGT+CGG	+	chr4.4:20585133-20585152	None:intergenic	20.0%
!!!	TATTTTTCCTTTGATTTAGT+GGG	-	chr4.4:20585215-20585234	MS.gene35044:CDS	20.0%
!!!	TTATTTTTCCTTTGATTTAG+TGG	-	chr4.4:20585214-20585233	MS.gene35044:CDS	20.0%
!	ATATTCTTGCATTATCCAAT+TGG	+	chr4.4:20584925-20584944	None:intergenic	25.0%
!!	ACAGAGATATTTAAAATGTC+GGG	+	chr4.4:20585132-20585151	None:intergenic	25.0%
!!!	TTTTGGTTGTGATTTTCTTT+TGG	+	chr4.4:20584988-20585007	None:intergenic	25.0%
	AAGATGATCAAGATGGAGAT+GGG	-	chr4.4:20585265-20585284	MS.gene35044:CDS	35.0%
	CATATCACCCACTAAATCAA+AGG	+	chr4.4:20585225-20585244	None:intergenic	35.0%
	CTAATGGTAACCAAACCAAT+TGG	-	chr4.4:20584907-20584926	MS.gene35044:CDS	35.0%
	CTTGCATTATCCAATTGGTT+TGG	+	chr4.4:20584920-20584939	None:intergenic	35.0%
!!	AATGTGCTGAGTTTTGTCAT+TGG	+	chr4.4:20585069-20585088	None:intergenic	35.0%
!!	CAATTGGTTTGGTTACCATT+AGG	+	chr4.4:20584909-20584928	None:intergenic	35.0%
	ACTTGTTCTTCCATTCCCAT+TGG	+	chr4.4:20584819-20584838	None:intergenic	40.0%
	AGCTCAAGCAATAAGAGAGT+TGG	-	chr4.4:20584846-20584865	MS.gene35044:CDS	40.0%
	CATTAGGTGCTTCTCCAATA+TGG	+	chr4.4:20584893-20584912	None:intergenic	40.0%
	CGTGATGAAGATGATCAAGA+TGG	-	chr4.4:20585258-20585277	MS.gene35044:CDS	40.0%
	GAAGATGATCAAGATGGAGA+TGG	-	chr4.4:20585264-20585283	MS.gene35044:CDS	40.0%
	TTTGGATGATGATCTGCTAG+AGG	+	chr4.4:20584970-20584989	None:intergenic	40.0%
!!	CATATTGGAGAAGCACCTAA+TGG	-	chr4.4:20584891-20584910	MS.gene35044:CDS	40.0%
	ACTTGCAATACCCCTCTTCT+CGG	+	chr4.4:20585097-20585116	None:intergenic	45.0%
	TGGGACAGAGCTATCTTGTT+TGG	-	chr4.4:20585284-20585303	MS.gene35044:CDS	45.0%
!!	ACATTAAGTGCCGAGAAGAG+GGG	-	chr4.4:20585084-20585103	MS.gene35044:intron	45.0%
!!	CACATTAAGTGCCGAGAAGA+GGG	-	chr4.4:20585083-20585102	MS.gene35044:intron	45.0%
!!	GCACATTAAGTGCCGAGAAG+AGG	-	chr4.4:20585082-20585101	MS.gene35044:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	20584813	20585359	20584813	ID=MS.gene35044
chr4.4	mRNA	20584813	20585359	20584813	ID=MS.gene35044.t1;Parent=MS.gene35044
chr4.4	exon	20585162	20585359	20585162	ID=MS.gene35044.t1.exon1;Parent=MS.gene35044.t1
chr4.4	CDS	20585162	20585359	20585162	ID=cds.MS.gene35044.t1;Parent=MS.gene35044.t1
chr4.4	exon	20584813	20584938	20584813	ID=MS.gene35044.t1.exon2;Parent=MS.gene35044.t1
chr4.4	CDS	20584813	20584938	20584813	ID=cds.MS.gene35044.t1;Parent=MS.gene35044.t1

Gene Sequence

>MS.gene35044

ATGCCAATGGGAATGGAAGAACAAGTTGTAACAAGCTCAAGCAATAAGAGAGTTGGTATGAAGAAATATTATGTGTTCCATATTGGAGAAGCACCTAATGGTAACCAAACCAATTGGATAATGCAAGAATATCGTCTATCAGATTCTGCTTCTTCCTCTAGCAGATCATCATCCAAAAGAAAATCACAACCAAAATCAGTTAGTATATTCTTAATATATTATTTAGTACATGAAATTTTTTATGTCAAATAGTCCAATGACAAAACTCAGCACATTAAGTGCCGAGAAGAGGGGTATTGCAAGTTTGAACTTACGTCCCGACATTTTAAATATCTCTGTAAATGTTTATCATCTGAGTTGCGTATACATGATTAATTTTTTTTTATTTAATATTGATTTTATTATTTTTCCTTTGATTTAGTGGGTGATATGTCGAGTTTATGAGCGTGATGAAGATGATCAAGATGGAGATGGGACAGAGCTATCTTGTTTGGATGAAGTTTTCTTGTCATTAGATGATCTTGATGAAGTAAGCTTGCCAAATTAG

Protein sequence

>MS.gene35044.t1

MPMGMEEQVVTSSSNKRVGMKKYYVFHIGEAPNGNQTNWIMQEYRLSDSASSSSRSSSKRKSQPKSWVICRVYERDEDDQDGDGTELSCLDEVFLSLDDLDEVSLPN