AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060716


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060716.t1 MTR_1g049170 87.259 259 31 2 1 258 133 390 2.67e-169 473
MS.gene060716.t1 MTR_3g092000 69.683 221 64 2 38 257 186 404 9.14e-106 313
MS.gene060716.t1 MTR_3g091990 62.162 222 77 4 35 253 170 387 4.83e-92 278
MS.gene060716.t1 MTR_2g047180 51.082 231 94 9 31 253 148 367 1.17e-65 209
MS.gene060716.t1 MTR_3g030080 50.673 223 95 8 37 253 160 373 2.65e-63 202
MS.gene060716.t1 MTR_3g029650 53.394 221 95 7 43 258 150 367 2.67e-61 206
MS.gene060716.t1 MTR_3g029650 42.982 228 114 8 37 254 531 752 1.53e-41 151
MS.gene060716.t1 MTR_6g018315 49.782 229 101 8 33 251 141 365 1.81e-56 186
MS.gene060716.t1 MTR_1g016540 56.284 183 65 7 83 253 1 180 1.94e-56 179
MS.gene060716.t1 MTR_3g030480 50.459 218 89 8 39 253 154 355 4.50e-56 183
MS.gene060716.t1 MTR_3g030440 50.459 218 89 8 39 253 155 356 4.92e-56 183
MS.gene060716.t1 MTR_6g018360 49.782 229 101 8 33 251 156 380 7.67e-56 184
MS.gene060716.t1 MTR_3g030380 48.624 218 94 7 39 253 156 358 5.49e-54 178
MS.gene060716.t1 MTR_3g030050 42.629 251 105 5 39 251 171 420 9.63e-53 177
MS.gene060716.t1 MTR_3g030360 47.059 221 81 8 36 253 165 352 1.62e-52 174
MS.gene060716.t1 MTR_6g018400 47.248 218 101 6 37 251 162 368 2.11e-51 172
MS.gene060716.t1 MTR_1g016570 44.033 243 108 8 21 253 137 361 5.98e-51 171
MS.gene060716.t1 MTR_6g018390 47.465 217 101 7 37 251 160 365 1.12e-50 170
MS.gene060716.t1 MTR_3g029640 44.907 216 109 6 43 255 159 367 1.15e-50 170
MS.gene060716.t1 MTR_3g037710 41.784 213 111 2 52 251 50 262 3.79e-49 163
MS.gene060716.t1 MTR_3g029750 46.018 226 104 8 36 254 174 388 4.70e-49 166
MS.gene060716.t1 MTR_6g027100 44.206 233 111 8 25 251 156 375 1.59e-48 166
MS.gene060716.t1 MTR_3g029620 43.578 218 110 5 44 254 164 375 1.73e-48 164
MS.gene060716.t1 MTR_1g026850 43.946 223 114 7 37 252 146 364 5.13e-48 163
MS.gene060716.t1 MTR_6g027040 44.206 233 111 8 25 251 156 375 6.65e-48 163
MS.gene060716.t1 MTR_3g029670 45.575 226 105 8 36 254 174 388 1.24e-47 162
MS.gene060716.t1 MTR_1g007260 44.796 221 107 9 33 247 172 383 7.12e-47 160
MS.gene060716.t1 MTR_3g030355 44.690 226 109 7 41 254 175 396 8.91e-47 160
MS.gene060716.t1 MTR_3g030560 41.964 224 115 6 40 257 164 378 1.98e-45 156
MS.gene060716.t1 MTR_3g029920 42.259 239 111 13 32 257 160 384 7.38e-43 150
MS.gene060716.t1 MTR_3g058460 42.308 208 108 5 55 251 169 375 9.44e-43 149
MS.gene060716.t1 MTR_3g029900 44.811 212 99 6 41 251 263 457 2.88e-42 150
MS.gene060716.t1 MTR_3g029800 40.271 221 113 5 41 255 152 359 3.43e-42 147
MS.gene060716.t1 MTR_3g047980 40.351 228 102 8 30 251 160 359 4.15e-41 145
MS.gene060716.t1 MTR_3g029840 44.393 214 98 7 41 253 163 356 4.06e-39 139
MS.gene060716.t1 MTR_3g029820 45.856 181 75 9 87 257 165 332 8.99e-39 138
MS.gene060716.t1 MTR_1g007250 49.701 167 72 7 83 244 1 160 1.32e-38 132
MS.gene060716.t1 MTR_5g078450 53.237 139 42 4 117 253 2 119 3.94e-37 127
MS.gene060716.t1 MTR_3g029950 43.556 225 96 9 41 253 163 368 2.17e-36 132
MS.gene060716.t1 MTR_5g042230 46.328 177 89 5 69 240 13 188 2.86e-36 128
MS.gene060716.t1 MTR_4g022880 48.872 133 62 3 125 251 39 171 1.29e-33 120
MS.gene060716.t1 MTR_6g018370 39.891 183 102 5 17 192 78 259 1.51e-32 120
MS.gene060716.t1 MTR_8g035995 67.391 92 28 2 93 183 2 92 6.56e-32 115
MS.gene060716.t1 MTR_1g007220 38.462 195 87 6 53 244 3 167 9.90e-31 112
MS.gene060716.t1 MTR_7g005990 39.151 212 97 6 51 254 172 359 2.82e-30 116
MS.gene060716.t1 MTR_3g029870 45.294 170 65 7 96 253 182 335 6.95e-27 106
MS.gene060716.t1 MTR_1g007200 45.192 104 43 6 138 237 1 94 2.02e-15 71.2
MS.gene060716.t1 MTR_3g029850 39.744 78 44 2 174 251 42 116 3.13e-13 65.1
MS.gene060716.t1 MTR_8g074610 28.079 203 121 8 63 251 204 395 6.07e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060716.t1 AT2G17030 34.599 237 127 9 39 251 158 390 1.87e-25 103
MS.gene060716.t1 AT2G17036 31.250 240 136 11 33 251 152 383 1.71e-20 90.1
MS.gene060716.t1 AT2G17036 31.250 240 136 11 33 251 153 384 1.72e-20 90.1
MS.gene060716.t1 AT5G60060 26.549 226 138 9 33 241 158 372 7.15e-12 65.1
MS.gene060716.t1 AT5G24040 22.897 214 154 7 47 252 75 285 2.59e-11 62.8
MS.gene060716.t1 AT5G24040 22.897 214 154 7 47 252 160 370 7.47e-11 62.0

Find 52 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTATGACTCTCATTGTTT+TGG 0.157074 1.3:-31655065 MS.gene060716:CDS
CGTCAGGATCCGACACCTTT+AGG 0.286759 1.3:-31655407 MS.gene060716:CDS
CTGACTACTTCAACTTCTTC+TGG 0.327644 1.3:-31654779 MS.gene060716:CDS
GTCAGGATCCGACACCTTTA+GGG 0.352646 1.3:-31655406 MS.gene060716:CDS
ACAATACTGGATGACCGTTT+TGG 0.352729 1.3:+31655306 None:intergenic
GTCGAAGAGCTTAAGGGATA+AGG 0.404239 1.3:-31654976 MS.gene060716:CDS
TCGCTGCAGTGAAACAATAC+TGG 0.407261 1.3:+31655293 None:intergenic
AATGGAAGTCGAAGAGCTTA+AGG 0.422589 1.3:-31654983 MS.gene060716:CDS
ATTGGTCGGACAGTTGCGTT+TGG 0.424683 1.3:-31655185 MS.gene060716:CDS
CCACATCGCATGTTATCAAA+AGG 0.430095 1.3:+31654855 None:intergenic
CAGAAGAAGTTGAAGTAGTC+AGG 0.435981 1.3:+31654780 None:intergenic
TCGGACAGTTGCGTTTGGAT+TGG 0.449462 1.3:-31655180 MS.gene060716:CDS
GGACTATAGTGTCGAGTTAG+TGG 0.460930 1.3:-31655159 MS.gene060716:CDS
CAACTTCTTCTGGCCACCTC+CGG 0.471703 1.3:-31654769 MS.gene060716:CDS
TAAGCTTAATGAGAAGAAGA+AGG 0.474353 1.3:-31655006 MS.gene060716:CDS
TCAGTTGAAGGTACAGCGTC+AGG 0.478522 1.3:-31655423 MS.gene060716:CDS
TTTCCCATGCCATACCCTAA+AGG 0.485274 1.3:+31655392 None:intergenic
GTTTGAACTCTATCAGTTGA+AGG 0.490865 1.3:-31655435 MS.gene060716:CDS
TTAATGAGAAGAAGAAGGAA+TGG 0.498438 1.3:-31655001 MS.gene060716:CDS
ATGGAAGTCGAAGAGCTTAA+GGG 0.506592 1.3:-31654982 MS.gene060716:CDS
TTCACTGCAGCGAAGAGCGT+TGG 0.509094 1.3:-31655283 MS.gene060716:CDS
TCGAGAATCCACTCCGGAGG+TGG 0.511300 1.3:+31654756 None:intergenic
AAAGACGACACAATTCCCTT+TGG 0.513520 1.3:+31654896 None:intergenic
TTTGGGTTCCCTGTCAAAGA+GGG 0.520426 1.3:-31655047 MS.gene060716:CDS
CAGATTTGTCTATTGCCAAA+GGG 0.522486 1.3:-31654911 MS.gene060716:CDS
AGAAAGTACGTTGACATGCA+TGG 0.524824 1.3:+31655251 None:intergenic
AGTCAGGATAATCAGACAAA+GGG 0.533962 1.3:+31654796 None:intergenic
TCAGATTTGTCTATTGCCAA+AGG 0.535639 1.3:-31654912 MS.gene060716:CDS
GATCCGACACCTTTAGGGTA+TGG 0.541589 1.3:-31655401 MS.gene060716:CDS
CTCGGGACATTGAACCAAAA+CGG 0.542934 1.3:-31655320 MS.gene060716:CDS
ACTCACCTTCACACTCTACA+AGG 0.545195 1.3:+31655105 None:intergenic
CACATCGCATGTTATCAAAA+GGG 0.547013 1.3:+31654856 None:intergenic
GAAAGTACGTTGACATGCAT+GGG 0.547996 1.3:+31655252 None:intergenic
AATCAGACAAAGGGATAAGC+CGG 0.556570 1.3:+31654805 None:intergenic
ATGCAGTTAATAAAATTGGT+CGG 0.557335 1.3:-31655199 MS.gene060716:CDS
CAAAGAGGGTCTAAAACTGA+AGG 0.576112 1.3:-31655033 MS.gene060716:CDS
TCTTCTGGCCACCTCCGGAG+TGG 0.587712 1.3:-31654764 MS.gene060716:CDS
ATGCCATACCCTAAAGGTGT+CGG 0.595533 1.3:+31655398 None:intergenic
AGGTGAGTTGTTGCTAGTAG+TGG 0.596067 1.3:-31655090 MS.gene060716:CDS
ATATCTGTGCGTTTAAAGAG+CGG 0.602087 1.3:-31655226 MS.gene060716:CDS
TAGTCAGGATAATCAGACAA+AGG 0.606271 1.3:+31654795 None:intergenic
TTAGTGGCTGAACATGTGAA+TGG 0.607438 1.3:-31655143 MS.gene060716:CDS
GACACCTTTAGGGTATGGCA+TGG 0.609897 1.3:-31655396 MS.gene060716:CDS
TGGCTGAACATGTGAATGGA+GGG 0.622410 1.3:-31655139 MS.gene060716:CDS
ACACCTTTAGGGTATGGCAT+GGG 0.643517 1.3:-31655395 MS.gene060716:CDS
GTGGCTGAACATGTGAATGG+AGG 0.661757 1.3:-31655140 MS.gene060716:CDS
ATTTCGAGAATCCACTCCGG+AGG 0.662099 1.3:+31654753 None:intergenic
AAATTCCTTGTAGAGTGTGA+AGG 0.674175 1.3:-31655110 MS.gene060716:CDS
TGCATTTCGAGAATCCACTC+CGG 0.678133 1.3:+31654750 None:intergenic
GGCTGAACATGTGAATGGAG+GGG 0.690996 1.3:-31655138 MS.gene060716:CDS
TAGTTGCTATTATGCAACGT+GGG 0.698080 1.3:-31655364 MS.gene060716:CDS
GTAGTTGCTATTATGCAACG+TGG 0.761838 1.3:-31655365 MS.gene060716:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGCAGTTAATAAAAT+TGG - chr1.3:31655025-31655044 MS.gene060716:CDS 15.0%
! ATGCAGTTAATAAAATTGGT+CGG - chr1.3:31655029-31655048 MS.gene060716:CDS 25.0%
AACAAAAAAACTGTTGCTCT+CGG - chr1.3:31654890-31654909 MS.gene060716:CDS 30.0%
TAAGCTTAATGAGAAGAAGA+AGG - chr1.3:31655222-31655241 MS.gene060716:CDS 30.0%
TTAATGAGAAGAAGAAGGAA+TGG - chr1.3:31655227-31655246 MS.gene060716:CDS 30.0%
! ATCTATGACTCTCATTGTTT+TGG - chr1.3:31655163-31655182 MS.gene060716:CDS 30.0%
! TCTATGACTCTCATTGTTTT+GGG - chr1.3:31655164-31655183 MS.gene060716:CDS 30.0%
!!! AAGATCAGACCGTTTTAAAA+CGG - chr1.3:31654753-31654772 MS.gene060716:CDS 30.0%
!!! TTCCTTAGACCGTTTTAAAA+CGG + chr1.3:31654765-31654784 None:intergenic 30.0%
AAATTCCTTGTAGAGTGTGA+AGG - chr1.3:31655118-31655137 MS.gene060716:CDS 35.0%
ACAAAAAAACTGTTGCTCTC+GGG - chr1.3:31654891-31654910 MS.gene060716:CDS 35.0%
AGTCAGGATAATCAGACAAA+GGG + chr1.3:31655435-31655454 None:intergenic 35.0%
ATATCTGTGCGTTTAAAGAG+CGG - chr1.3:31655002-31655021 MS.gene060716:CDS 35.0%
CACATCGCATGTTATCAAAA+GGG + chr1.3:31655375-31655394 None:intergenic 35.0%
CAGATTTGTCTATTGCCAAA+GGG - chr1.3:31655317-31655336 MS.gene060716:CDS 35.0%
GTTTGAACTCTATCAGTTGA+AGG - chr1.3:31654793-31654812 MS.gene060716:CDS 35.0%
TAGTCAGGATAATCAGACAA+AGG + chr1.3:31655436-31655455 None:intergenic 35.0%
TCAGATTTGTCTATTGCCAA+AGG - chr1.3:31655316-31655335 MS.gene060716:CDS 35.0%
! TAGTTGCTATTATGCAACGT+GGG - chr1.3:31654864-31654883 MS.gene060716:CDS 35.0%
! TGGGATGTGTGTTTTTCATT+TGG - chr1.3:31655393-31655412 MS.gene060716:CDS 35.0%
! TTTTTCATTTGGACGAACAC+CGG - chr1.3:31655404-31655423 MS.gene060716:CDS 35.0%
!! CTTTTGATAACATGCGATGT+GGG - chr1.3:31655374-31655393 MS.gene060716:CDS 35.0%
!!! AAGGGATAAGGTTTTGTTCT+TGG - chr1.3:31655264-31655283 MS.gene060716:CDS 35.0%
!!! AAGGTTTTGTTCTTGGGAAA+TGG - chr1.3:31655271-31655290 MS.gene060716:CDS 35.0%
!!! AGGGATAAGGTTTTGTTCTT+GGG - chr1.3:31655265-31655284 MS.gene060716:CDS 35.0%
AAAGACGACACAATTCCCTT+TGG + chr1.3:31655335-31655354 None:intergenic 40.0%
AATCAGACAAAGGGATAAGC+CGG + chr1.3:31655426-31655445 None:intergenic 40.0%
AATGGAAGTCGAAGAGCTTA+AGG - chr1.3:31655245-31655264 MS.gene060716:CDS 40.0%
ATGGAAGTCGAAGAGCTTAA+GGG - chr1.3:31655246-31655265 MS.gene060716:CDS 40.0%
CAGAAGAAGTTGAAGTAGTC+AGG + chr1.3:31655451-31655470 None:intergenic 40.0%
CCACATCGCATGTTATCAAA+AGG + chr1.3:31655376-31655395 None:intergenic 40.0%
CTGACTACTTCAACTTCTTC+TGG - chr1.3:31655449-31655468 MS.gene060716:CDS 40.0%
TTAGTGGCTGAACATGTGAA+TGG - chr1.3:31655085-31655104 MS.gene060716:CDS 40.0%
! ACAATACTGGATGACCGTTT+TGG + chr1.3:31654925-31654944 None:intergenic 40.0%
! AGAAAGTACGTTGACATGCA+TGG + chr1.3:31654980-31654999 None:intergenic 40.0%
! CAAAGAGGGTCTAAAACTGA+AGG - chr1.3:31655195-31655214 MS.gene060716:CDS 40.0%
! GAAAGTACGTTGACATGCAT+GGG + chr1.3:31654979-31654998 None:intergenic 40.0%
! GTAGTTGCTATTATGCAACG+TGG - chr1.3:31654863-31654882 MS.gene060716:CDS 40.0%
!! CCTTTTGATAACATGCGATG+TGG - chr1.3:31655373-31655392 MS.gene060716:CDS 40.0%
!! GTTTTAGACCCTCTTTGACA+GGG + chr1.3:31655192-31655211 None:intergenic 40.0%
!!! AGTTTTAGACCCTCTTTGAC+AGG + chr1.3:31655193-31655212 None:intergenic 40.0%
!!! GACCGTTTTAAAACGGTCTA+AGG - chr1.3:31654760-31654779 MS.gene060716:CDS 40.0%
ACTCACCTTCACACTCTACA+AGG + chr1.3:31655126-31655145 None:intergenic 45.0%
AGGGTATGGCATGGGAAAAA+AGG - chr1.3:31654841-31654860 MS.gene060716:CDS 45.0%
AGGTGAGTTGTTGCTAGTAG+TGG - chr1.3:31655138-31655157 MS.gene060716:CDS 45.0%
CTCGGGACATTGAACCAAAA+CGG - chr1.3:31654908-31654927 MS.gene060716:CDS 45.0%
GTCGAAGAGCTTAAGGGATA+AGG - chr1.3:31655252-31655271 MS.gene060716:CDS 45.0%
TCGCTGCAGTGAAACAATAC+TGG + chr1.3:31654938-31654957 None:intergenic 45.0%
TGCATTTCGAGAATCCACTC+CGG + chr1.3:31655481-31655500 None:intergenic 45.0%
TGGCTGAACATGTGAATGGA+GGG - chr1.3:31655089-31655108 MS.gene060716:CDS 45.0%
TTTCCCATGCCATACCCTAA+AGG + chr1.3:31654839-31654858 None:intergenic 45.0%
! ATGCCATACCCTAAAGGTGT+CGG + chr1.3:31654833-31654852 None:intergenic 45.0%
! GGACTATAGTGTCGAGTTAG+TGG - chr1.3:31655069-31655088 MS.gene060716:CDS 45.0%
! TTTGGGTTCCCTGTCAAAGA+GGG - chr1.3:31655181-31655200 MS.gene060716:CDS 45.0%
!! ACACCTTTAGGGTATGGCAT+GGG - chr1.3:31654833-31654852 MS.gene060716:CDS 45.0%
!!! TTTTGGGTTCCCTGTCAAAG+AGG - chr1.3:31655180-31655199 MS.gene060716:CDS 45.0%
ATTGGTCGGACAGTTGCGTT+TGG - chr1.3:31655043-31655062 MS.gene060716:CDS 50.0%
ATTTCGAGAATCCACTCCGG+AGG + chr1.3:31655478-31655497 None:intergenic 50.0%
GGCTGAACATGTGAATGGAG+GGG - chr1.3:31655090-31655109 MS.gene060716:CDS 50.0%
GTCAGGATCCGACACCTTTA+GGG - chr1.3:31654822-31654841 MS.gene060716:CDS 50.0%
GTGGCTGAACATGTGAATGG+AGG - chr1.3:31655088-31655107 MS.gene060716:CDS 50.0%
TCGGACAGTTGCGTTTGGAT+TGG - chr1.3:31655048-31655067 MS.gene060716:CDS 50.0%
! TCAGTTGAAGGTACAGCGTC+AGG - chr1.3:31654805-31654824 MS.gene060716:CDS 50.0%
!! GACACCTTTAGGGTATGGCA+TGG - chr1.3:31654832-31654851 MS.gene060716:CDS 50.0%
!! GATCCGACACCTTTAGGGTA+TGG - chr1.3:31654827-31654846 MS.gene060716:CDS 50.0%
CAACTTCTTCTGGCCACCTC+CGG - chr1.3:31655459-31655478 MS.gene060716:CDS 55.0%
CGTCAGGATCCGACACCTTT+AGG - chr1.3:31654821-31654840 MS.gene060716:CDS 55.0%
! TTCACTGCAGCGAAGAGCGT+TGG - chr1.3:31654945-31654964 MS.gene060716:CDS 55.0%
TCGAGAATCCACTCCGGAGG+TGG + chr1.3:31655475-31655494 None:intergenic 60.0%
TCTTCTGGCCACCTCCGGAG+TGG - chr1.3:31655464-31655483 MS.gene060716:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 31654737 31655513 31654737 ID=MS.gene060716
chr1.3 mRNA 31654737 31655513 31654737 ID=MS.gene060716.t1;Parent=MS.gene060716
chr1.3 exon 31654737 31655513 31654737 ID=MS.gene060716.t1.exon1;Parent=MS.gene060716.t1
chr1.3 CDS 31654737 31655513 31654737 ID=cds.MS.gene060716.t1;Parent=MS.gene060716.t1
Gene Sequence

>MS.gene060716

ATGGGAAGATATAATGAAGATCAGACCGTTTTAAAACGGTCTAAGGAAGTATCGCAGTTTGAACTCTATCAGTTGAAGGTACAGCGTCAGGATCCGACACCTTTAGGGTATGGCATGGGAAAAAAGGTAGTTGCTATTATGCAACGTGGGAAAAACAAAAAAACTGTTGCTCTCGGGACATTGAACCAAAACGGTCATCCAGTATTGTTTCACTGCAGCGAAGAGCGTTGGACGTTGATCCCATGCATGTCAACGTACTTTCTAGATATCTGTGCGTTTAAAGAGCGGTTTTATGCAGTTAATAAAATTGGTCGGACAGTTGCGTTTGGATTGGACTATAGTGTCGAGTTAGTGGCTGAACATGTGAATGGAGGGGACATGAAATTCCTTGTAGAGTGTGAAGGTGAGTTGTTGCTAGTAGTGGACATCTATGACTCTCATTGTTTTGGGTTCCCTGTCAAAGAGGGTCTAAAACTGAAGGTGTTTAAGCTTAATGAGAAGAAGAAGGAATGGAAGTCGAAGAGCTTAAGGGATAAGGTTTTGTTCTTGGGAAATGGATGTTCGTTTACTGCTTCTGCTTCAGATTTGTCTATTGCCAAAGGGAATTGTGTCGTCTTTATTGACGATGCATTCATCCCTTTTGATAACATGCGATGTGGGATGTGTGTTTTTCATTTGGACGAACACCGGCTTATCCCTTTGTCTGATTATCCTGACTACTTCAACTTCTTCTGGCCACCTCCGGAGTGGATTCTCGAAATGCAATACCATTCATAA

Protein sequence

>MS.gene060716.t1

MGRYNEDQTVLKRSKEVSQFELYQLKVQRQDPTPLGYGMGKKVVAIMQRGKNKKTVALGTLNQNGHPVLFHCSEERWTLIPCMSTYFLDICAFKERFYAVNKIGRTVAFGLDYSVELVAEHVNGGDMKFLVECEGELLLVVDIYDSHCFGFPVKEGLKLKVFKLNEKKKEWKSKSLRDKVLFLGNGCSFTASASDLSIAKGNCVVFIDDAFIPFDNMRCGMCVFHLDEHRLIPLSDYPDYFNFFWPPPEWILEMQYHS