Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35211.t1 | XP_013457403.1 | 94.8 | 115 | 6 | 0 | 1 | 115 | 1 | 115 | 1.20E-46 | 195.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35211.t1 | A0A072UNP9 | 94.8 | 115 | 6 | 0 | 1 | 115 | 1 | 115 | 8.7e-47 | 195.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049580 | MS.gene35211 | 0.805827 | 1.11E-49 | -1.69E-46 |
| MS.gene049861 | MS.gene35211 | 0.816541 | 5.24E-52 | -1.69E-46 |
| MS.gene050277 | MS.gene35211 | 0.805875 | 1.08E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene35211 | 0.804748 | 1.86E-49 | -1.69E-46 |
| MS.gene050320 | MS.gene35211 | 0.836339 | 9.92E-57 | -1.69E-46 |
| MS.gene050752 | MS.gene35211 | 0.819836 | 9.42E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene35211 | 0.819251 | 1.28E-52 | -1.69E-46 |
| MS.gene051063 | MS.gene35211 | 0.822384 | 2.44E-53 | -1.69E-46 |
| MS.gene051245 | MS.gene35211 | 0.823194 | 1.58E-53 | -1.69E-46 |
| MS.gene051746 | MS.gene35211 | 0.837655 | 4.57E-57 | -1.69E-46 |
| MS.gene051748 | MS.gene35211 | 0.833861 | 4.18E-56 | -1.69E-46 |
| MS.gene051902 | MS.gene35211 | 0.801498 | 8.75E-49 | -1.69E-46 |
| MS.gene051903 | MS.gene35211 | 0.837986 | 3.76E-57 | -1.69E-46 |
| MS.gene052434 | MS.gene35211 | 0.801824 | 7.50E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene35211 | 0.818276 | 2.13E-52 | -1.69E-46 |
| MS.gene053109 | MS.gene35211 | 0.827489 | 1.52E-54 | -1.69E-46 |
| MS.gene054403 | MS.gene35211 | 0.819929 | 8.97E-53 | -1.69E-46 |
| MS.gene05469 | MS.gene35211 | 0.816231 | 6.15E-52 | -1.69E-46 |
| MS.gene056752 | MS.gene35211 | 0.856023 | 4.26E-62 | -1.69E-46 |
| MS.gene056764 | MS.gene35211 | 0.816622 | 5.03E-52 | -1.69E-46 |
| MS.gene057057 | MS.gene35211 | 0.801684 | 8.02E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene35211 | 0.824796 | 6.65E-54 | -1.69E-46 |
| MS.gene057234 | MS.gene35211 | 0.83213 | 1.13E-55 | -1.69E-46 |
| MS.gene057452 | MS.gene35211 | -0.822165 | 2.74E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene35211 | 0.850229 | 1.94E-60 | -1.69E-46 |
| MS.gene058074 | MS.gene35211 | 0.832541 | 8.92E-56 | -1.69E-46 |
| MS.gene058238 | MS.gene35211 | 0.808035 | 3.77E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene35211 | 0.816377 | 5.71E-52 | -1.69E-46 |
| MS.gene06011 | MS.gene35211 | 0.841469 | 4.66E-58 | -1.69E-46 |
| MS.gene060763 | MS.gene35211 | 0.833667 | 4.67E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35211.t1 | MTR_4g094782 | 94.783 | 115 | 6 | 0 | 1 | 115 | 1 | 115 | 2.30e-55 | 168 |
| MS.gene35211.t1 | MTR_4g094782 | 89.855 | 69 | 7 | 0 | 1 | 69 | 1 | 69 | 2.32e-27 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAGAGGGAGAAGAGGCTT+AGG | 0.363591 | 4.4:+18628499 | MS.gene35211:CDS |
| TTGAAGCCTGTCGGTAGAAT+AGG | 0.372645 | 4.4:+18629863 | MS.gene35211:CDS |
| CATATGTGTCGCTTTCTACT+TGG | 0.419475 | 4.4:-18629947 | None:intergenic |
| ACTTCCATGGTAGTATTTGA+TGG | 0.421228 | 4.4:-18628627 | None:intergenic |
| TTGGCTTTGAATTACCATTC+CGG | 0.434854 | 4.4:-18629928 | None:intergenic |
| TGAAGCCTGTCGGTAGAATA+GGG | 0.439503 | 4.4:+18629864 | MS.gene35211:CDS |
| GCTTCTTTCTTGTCATAGAA+AGG | 0.440776 | 4.4:-18628546 | None:intergenic |
| TTCTTCCCTATTCTACCGAC+AGG | 0.441878 | 4.4:-18629869 | None:intergenic |
| TATTCTACCGACAGGCTTCA+AGG | 0.456173 | 4.4:-18629861 | None:intergenic |
| GGCTGAGAAACTTGAAGCTT+AGG | 0.465133 | 4.4:-18628606 | None:intergenic |
| AAGAAGTTTAAAATGGCTAA+AGG | 0.520775 | 4.4:+18629899 | MS.gene35211:CDS |
| CAAGATGTGGAGATGGCTGA+TGG | 0.544852 | 4.4:+18629821 | MS.gene35211:intron |
| TGAGATCGAAGAGGGAGAAG+AGG | 0.548294 | 4.4:+18628493 | MS.gene35211:CDS |
| GAAGAGGGAGAAGAGGCTTA+GGG | 0.556941 | 4.4:+18628500 | MS.gene35211:CDS |
| GCTAAAGGCAACCGCCGGAA+TGG | 0.562871 | 4.4:+18629914 | MS.gene35211:CDS |
| AACTATCAACAACAACGCCA+TGG | 0.565201 | 4.4:+18628659 | MS.gene35211:CDS |
| AGTTGAAGTGGAAACTTCCA+TGG | 0.576743 | 4.4:-18628640 | None:intergenic |
| GAAGTCAATGAGATCGAAGA+GGG | 0.602910 | 4.4:+18628485 | MS.gene35211:CDS |
| ACTATCAACAACAACGCCAT+GGG | 0.608842 | 4.4:+18628660 | MS.gene35211:CDS |
| GTTGTTGTTGATAGTTGAAG+TGG | 0.619350 | 4.4:-18628652 | None:intergenic |
| AAATGGCTAAAGGCAACCGC+CGG | 0.626265 | 4.4:+18629909 | MS.gene35211:CDS |
| AAGACTTCCTTGAAGCCTGT+CGG | 0.641141 | 4.4:+18629854 | MS.gene35211:CDS |
| CGAAGTCAATGAGATCGAAG+AGG | 0.658126 | 4.4:+18628484 | MS.gene35211:CDS |
| TCAGCCATCAAATACTACCA+TGG | 0.659640 | 4.4:+18628623 | MS.gene35211:CDS |
| GCTTTGAATTACCATTCCGG+CGG | 0.686125 | 4.4:-18629925 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAAAATAATATAAAAT+TGG | - | chr4.4:18629736-18629755 | None:intergenic | 0.0% |
| !! | AATAATAAAAAAGAAAAAAG+TGG | - | chr4.4:18628688-18628707 | None:intergenic | 10.0% |
| !!! | ATTTATCCTATTTAAAATAT+TGG | - | chr4.4:18629041-18629060 | None:intergenic | 10.0% |
| !! | AGTAATAATATATCTATCTA+TGG | - | chr4.4:18629227-18629246 | None:intergenic | 15.0% |
| !! | ATTGAAAAAGAAGTTTAAAA+TGG | + | chr4.4:18629892-18629911 | MS.gene35211:CDS | 15.0% |
| !!! | TTTTTCTTTAATTTCATAGT+TGG | + | chr4.4:18629547-18629566 | MS.gene35211:intron | 15.0% |
| !! | AATATATCTATCTATGGAAT+TGG | - | chr4.4:18629221-18629240 | None:intergenic | 20.0% |
| !! | ATAATCATAATAGCTTCAAA+AGG | - | chr4.4:18629496-18629515 | None:intergenic | 20.0% |
| !! | ATATATCTATCTATGGAATT+GGG | - | chr4.4:18629220-18629239 | None:intergenic | 20.0% |
| !! | ATTTACTTAGTACAAATGTA+GGG | + | chr4.4:18628785-18628804 | MS.gene35211:intron | 20.0% |
| !! | TATTTACTTAGTACAAATGT+AGG | + | chr4.4:18628784-18628803 | MS.gene35211:intron | 20.0% |
| !! | TTTAATTATGAATTGGCATT+TGG | + | chr4.4:18629748-18629767 | MS.gene35211:intron | 20.0% |
| !!! | AAAACAAAAATGGGTTTTTA+GGG | - | chr4.4:18629465-18629484 | None:intergenic | 20.0% |
| ! | AAAAAATAAGCCAATCCAAA+CGG | + | chr4.4:18629013-18629032 | MS.gene35211:intron | 25.0% |
| ! | AAAAATAAGTCAATCGAAAC+GGG | - | chr4.4:18628837-18628856 | None:intergenic | 25.0% |
| ! | AAAACCCAATTCAATTTGAA+AGG | - | chr4.4:18629398-18629417 | None:intergenic | 25.0% |
| ! | AACTACTAAAACACTAAAAG+TGG | - | chr4.4:18629139-18629158 | None:intergenic | 25.0% |
| ! | ATAGTCCTTTCAAATTGAAT+TGG | + | chr4.4:18629390-18629409 | MS.gene35211:intron | 25.0% |
| ! | CAAAAATAAGTCAATCGAAA+CGG | - | chr4.4:18628838-18628857 | None:intergenic | 25.0% |
| ! | GCAAAAATCAACATTTACAA+TGG | + | chr4.4:18628810-18628829 | MS.gene35211:intron | 25.0% |
| ! | GCTTAGAAGAAAACAAAAAT+GGG | - | chr4.4:18629474-18629493 | None:intergenic | 25.0% |
| ! | TAGTCCTTTCAAATTGAATT+GGG | + | chr4.4:18629391-18629410 | MS.gene35211:intron | 25.0% |
| ! | TTCAAAAGGAAAAACAACAA+AGG | - | chr4.4:18629613-18629632 | None:intergenic | 25.0% |
| !! | AAGAAGTTTAAAATGGCTAA+AGG | + | chr4.4:18629899-18629918 | MS.gene35211:CDS | 25.0% |
| !! | ATGTTATCTCTTTAGTTTAG+CGG | + | chr4.4:18628724-18628743 | MS.gene35211:intron | 25.0% |
| !!! | GAAAACAAAAATGGGTTTTT+AGG | - | chr4.4:18629466-18629485 | None:intergenic | 25.0% |
| !!! | GTTTTTGAATTTTCAAGATG+TGG | + | chr4.4:18629808-18629827 | MS.gene35211:intron | 25.0% |
| AAAAATAAGCCAATCCAAAC+GGG | + | chr4.4:18629014-18629033 | MS.gene35211:intron | 30.0% | |
| GAAAGAGAAAGAGAAAAAGA+AGG | - | chr4.4:18629366-18629385 | None:intergenic | 30.0% | |
| GGCTTAGAAGAAAACAAAAA+TGG | - | chr4.4:18629475-18629494 | None:intergenic | 30.0% | |
| TGTTATTCATAAGCTTGTCA+AGG | - | chr4.4:18628965-18628984 | None:intergenic | 30.0% | |
| ! | CCATCACTCATTTTCTATAA+GGG | - | chr4.4:18629082-18629101 | None:intergenic | 30.0% |
| ! | TCCATCACTCATTTTCTATA+AGG | - | chr4.4:18629083-18629102 | None:intergenic | 30.0% |
| ! | TTTAAAATATTGGACCCGTT+TGG | - | chr4.4:18629031-18629050 | None:intergenic | 30.0% |
| ! | TTTCATAGTTGGAGTTTTCT+TGG | + | chr4.4:18629558-18629577 | MS.gene35211:intron | 30.0% |
| !! | ACGGGTCCAATATTTTAAAT+AGG | + | chr4.4:18629032-18629051 | MS.gene35211:intron | 30.0% |
| !!! | CTTGTTTGTGTTGTTTGTTT+TGG | - | chr4.4:18629262-18629281 | None:intergenic | 30.0% |
| !!! | TTGTGTTGTTTGTTTTGGAA+GGG | - | chr4.4:18629257-18629276 | None:intergenic | 30.0% |
| !!! | TTTGTGTTGTTTGTTTTGGA+AGG | - | chr4.4:18629258-18629277 | None:intergenic | 30.0% |
| AAAGAAAAAAGTGGTACCCA+TGG | - | chr4.4:18628679-18628698 | None:intergenic | 35.0% | |
| AACAACACAAACAAGAAGCT+TGG | + | chr4.4:18629267-18629286 | MS.gene35211:intron | 35.0% | |
| ACACTAAAAGTGGAGAACAA+AGG | - | chr4.4:18629129-18629148 | None:intergenic | 35.0% | |
| ACTTCCATGGTAGTATTTGA+TGG | - | chr4.4:18628630-18628649 | None:intergenic | 35.0% | |
| CCCTTATAGAAAATGAGTGA+TGG | + | chr4.4:18629079-18629098 | MS.gene35211:intron | 35.0% | |
| GCTTCTTTCTTGTCATAGAA+AGG | - | chr4.4:18628549-18628568 | None:intergenic | 35.0% | |
| !! | GAATTTTCAAGATGTGGAGA+TGG | + | chr4.4:18629814-18629833 | MS.gene35211:intron | 35.0% |
| !! | GTTGTTGTTGATAGTTGAAG+TGG | - | chr4.4:18628655-18628674 | None:intergenic | 35.0% |
| !! | TTGGCTTTGAATTACCATTC+CGG | - | chr4.4:18629931-18629950 | None:intergenic | 35.0% |
| !! | TTTTGAAAGATCTGTCACAG+TGG | - | chr4.4:18629425-18629444 | None:intergenic | 35.0% |
| AACTATCAACAACAACGCCA+TGG | + | chr4.4:18628659-18628678 | MS.gene35211:CDS | 40.0% | |
| AATATTGGACCCGTTTGGAT+TGG | - | chr4.4:18629026-18629045 | None:intergenic | 40.0% | |
| ACAAGAAGCTTGGAACTTGA+AGG | + | chr4.4:18629277-18629296 | MS.gene35211:intron | 40.0% | |
| ACTATCAACAACAACGCCAT+GGG | + | chr4.4:18628660-18628679 | MS.gene35211:CDS | 40.0% | |
| AGTTGAAGTGGAAACTTCCA+TGG | - | chr4.4:18628643-18628662 | None:intergenic | 40.0% | |
| ATAAAACACGCTGTCTTCGT+TGG | - | chr4.4:18629685-18629704 | None:intergenic | 40.0% | |
| GAAGTCAATGAGATCGAAGA+GGG | + | chr4.4:18628485-18628504 | MS.gene35211:CDS | 40.0% | |
| TAATCACTGCCCTACTCTTT+CGG | - | chr4.4:18629170-18629189 | None:intergenic | 40.0% | |
| TCAGCCATCAAATACTACCA+TGG | + | chr4.4:18628623-18628642 | MS.gene35211:CDS | 40.0% | |
| TGAATTGGCATTTGGTTGCT+TGG | + | chr4.4:18629756-18629775 | MS.gene35211:intron | 40.0% | |
| TTCGTTGGAGTAGTCAATCA+CGG | - | chr4.4:18629670-18629689 | None:intergenic | 40.0% | |
| ! | ACCTTTTCATCAAAGGGGTT+TGG | + | chr4.4:18629310-18629329 | MS.gene35211:intron | 40.0% |
| ! | CCAACACCTTTTCATCAAAG+GGG | + | chr4.4:18629305-18629324 | MS.gene35211:intron | 40.0% |
| ! | GCCAACACCTTTTCATCAAA+GGG | + | chr4.4:18629304-18629323 | MS.gene35211:intron | 40.0% |
| ! | TGCCAACACCTTTTCATCAA+AGG | + | chr4.4:18629303-18629322 | MS.gene35211:intron | 40.0% |
| !! | CATATGTGTCGCTTTCTACT+TGG | - | chr4.4:18629950-18629969 | None:intergenic | 40.0% |
| !! | TTCCTTTTGAAGATTCAGCG+CGG | + | chr4.4:18629622-18629641 | MS.gene35211:intron | 40.0% |
| !!! | AACAACAAAGGTGTTTTCCC+CGG | - | chr4.4:18629601-18629620 | None:intergenic | 40.0% |
| !!! | CACTGATTTTGTTTTTGCCG+GGG | + | chr4.4:18629581-18629600 | MS.gene35211:intron | 40.0% |
| !!! | GCACTGATTTTGTTTTTGCC+GGG | + | chr4.4:18629580-18629599 | MS.gene35211:intron | 40.0% |
| !!! | GGCACTGATTTTGTTTTTGC+CGG | + | chr4.4:18629579-18629598 | MS.gene35211:intron | 40.0% |
| AAGACTTCCTTGAAGCCTGT+CGG | + | chr4.4:18629854-18629873 | MS.gene35211:CDS | 45.0% | |
| CGAAGTCAATGAGATCGAAG+AGG | + | chr4.4:18628484-18628503 | MS.gene35211:CDS | 45.0% | |
| GCCAAACCCCTTTGATGAAA+AGG | - | chr4.4:18629314-18629333 | None:intergenic | 45.0% | |
| GGCAGTGATTAGAAAGAGAG+AGG | + | chr4.4:18629179-18629198 | MS.gene35211:intron | 45.0% | |
| GGCTGAGAAACTTGAAGCTT+AGG | - | chr4.4:18628609-18628628 | None:intergenic | 45.0% | |
| TATTCTACCGACAGGCTTCA+AGG | - | chr4.4:18629864-18629883 | None:intergenic | 45.0% | |
| TGAAGCCTGTCGGTAGAATA+GGG | + | chr4.4:18629864-18629883 | MS.gene35211:CDS | 45.0% | |
| TTCTTCCCTATTCTACCGAC+AGG | - | chr4.4:18629872-18629891 | None:intergenic | 45.0% | |
| TTGAAGCCTGTCGGTAGAAT+AGG | + | chr4.4:18629863-18629882 | MS.gene35211:CDS | 45.0% | |
| ! | CCCCTTTGATGAAAAGGTGT+TGG | - | chr4.4:18629308-18629327 | None:intergenic | 45.0% |
| ! | GCTTTGAATTACCATTCCGG+CGG | - | chr4.4:18629928-18629947 | None:intergenic | 45.0% |
| ! | TCATCAAAGGGGTTTGGCTT+TGG | + | chr4.4:18629316-18629335 | MS.gene35211:intron | 45.0% |
| !!! | ATATTATTTTAATTATGAAT+TGG | + | chr4.4:18629741-18629760 | MS.gene35211:intron | 5.0% |
| AAATGGCTAAAGGCAACCGC+CGG | + | chr4.4:18629909-18629928 | MS.gene35211:CDS | 50.0% | |
| AGAAAGAGAGAGGACTCGAG+TGG | + | chr4.4:18629189-18629208 | MS.gene35211:intron | 50.0% | |
| CAAGATGTGGAGATGGCTGA+TGG | + | chr4.4:18629821-18629840 | MS.gene35211:intron | 50.0% | |
| GAAAGAGAGAGGACTCGAGT+GGG | + | chr4.4:18629190-18629209 | MS.gene35211:intron | 50.0% | |
| GAAGAGGGAGAAGAGGCTTA+GGG | + | chr4.4:18628500-18628519 | MS.gene35211:CDS | 50.0% | |
| GTCCGCGCTGAATCTTCAAA+AGG | - | chr4.4:18629627-18629646 | None:intergenic | 50.0% | |
| TGAGATCGAAGAGGGAGAAG+AGG | + | chr4.4:18628493-18628512 | MS.gene35211:CDS | 50.0% | |
| TTTGCCGAGCCGAAAGAGTA+GGG | + | chr4.4:18629158-18629177 | MS.gene35211:intron | 50.0% | |
| ! | TTTTGCCGAGCCGAAAGAGT+AGG | + | chr4.4:18629157-18629176 | MS.gene35211:intron | 50.0% |
| ACTGCCCTACTCTTTCGGCT+CGG | - | chr4.4:18629165-18629184 | None:intergenic | 55.0% | |
| CGAAGAGGGAGAAGAGGCTT+AGG | + | chr4.4:18628499-18628518 | MS.gene35211:CDS | 55.0% | |
| GCTAAAGGCAACCGCCGGAA+TGG | + | chr4.4:18629914-18629933 | MS.gene35211:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 18628480 | 18629970 | 18628480 | ID=MS.gene35211 |
| chr4.4 | mRNA | 18628480 | 18629970 | 18628480 | ID=MS.gene35211.t1;Parent=MS.gene35211 |
| chr4.4 | exon | 18628480 | 18628681 | 18628480 | ID=MS.gene35211.t1.exon1;Parent=MS.gene35211.t1 |
| chr4.4 | CDS | 18628480 | 18628681 | 18628480 | ID=cds.MS.gene35211.t1;Parent=MS.gene35211.t1 |
| chr4.4 | exon | 18629825 | 18629970 | 18629825 | ID=MS.gene35211.t1.exon2;Parent=MS.gene35211.t1 |
| chr4.4 | CDS | 18629825 | 18629970 | 18629825 | ID=cds.MS.gene35211.t1;Parent=MS.gene35211.t1 |
| Gene Sequence |
| Protein sequence |
