AlfalfaGEDB | MS.gene35590

M. sativa cultivar XinJiangDaYe / MS.gene35590

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005787 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009987 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0032991 GO:0034641 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:0098796 GO:0110165 GO:0140096 GO:1901564 GO:1902494 GO:1905368
PFAM: PF06645.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35590.t1	KEH34932.1	97.4	78	2	0	1	78	1	78	2.00E-35	157.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35590.t1	Q7XI45	47.2	72	38	0	1	72	1	72	3.4e-12	72.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35590.t1	A0A072V0P5	97.4	78	2	0	1	78	1	78	1.4e-35	157.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene35590	0.818715	1.70E-52	-1.69E-46
MS.gene049382	MS.gene35590	0.82431	8.65E-54	-1.69E-46
MS.gene049758	MS.gene35590	0.836262	1.04E-56	-1.69E-46
MS.gene049915	MS.gene35590	0.807163	5.78E-50	-1.69E-46
MS.gene050128	MS.gene35590	0.835011	2.15E-56	-1.69E-46
MS.gene050277	MS.gene35590	0.807218	5.63E-50	-1.69E-46
MS.gene050762	MS.gene35590	0.813917	2.01E-51	-1.69E-46
MS.gene050763	MS.gene35590	0.814189	1.75E-51	-1.69E-46
MS.gene050987	MS.gene35590	0.808922	2.44E-50	-1.69E-46
MS.gene051243	MS.gene35590	0.825521	4.48E-54	-1.69E-46
MS.gene051535	MS.gene35590	0.809375	1.95E-50	-1.69E-46
MS.gene051626	MS.gene35590	0.817459	3.26E-52	-1.69E-46
MS.gene051662	MS.gene35590	0.820871	5.45E-53	-1.69E-46
MS.gene051663	MS.gene35590	0.81181	5.81E-51	-1.69E-46
MS.gene051789	MS.gene35590	0.849324	3.48E-60	-1.69E-46
MS.gene052178	MS.gene35590	0.81525	1.02E-51	-1.69E-46
MS.gene052286	MS.gene35590	0.8002	1.61E-48	-1.69E-46
MS.gene052302	MS.gene35590	0.810018	1.42E-50	-1.69E-46
MS.gene052337	MS.gene35590	0.816304	5.93E-52	-1.69E-46
MS.gene052376	MS.gene35590	0.803605	3.22E-49	-1.69E-46
MS.gene052967	MS.gene35590	0.831408	1.70E-55	-1.69E-46
MS.gene053074	MS.gene35590	0.839214	1.81E-57	-1.69E-46
MS.gene053559	MS.gene35590	0.803829	2.89E-49	-1.69E-46
MS.gene053630	MS.gene35590	0.810504	1.12E-50	-1.69E-46
MS.gene053836	MS.gene35590	0.806978	6.33E-50	-1.69E-46
MS.gene054184	MS.gene35590	0.82467	7.12E-54	-1.69E-46
MS.gene054185	MS.gene35590	0.827857	1.24E-54	-1.69E-46
MS.gene054379	MS.gene35590	0.804865	1.76E-49	-1.69E-46
MS.gene054596	MS.gene35590	0.811094	8.31E-51	-1.69E-46
MS.gene055133	MS.gene35590	0.870631	1.26E-66	-1.69E-46
MS.gene055207	MS.gene35590	0.843871	1.07E-58	-1.69E-46
MS.gene056760	MS.gene35590	0.806141	9.50E-50	-1.69E-46
MS.gene057754	MS.gene35590	0.870591	1.30E-66	-1.69E-46
MS.gene058749	MS.gene35590	0.800198	1.61E-48	-1.69E-46
MS.gene058833	MS.gene35590	0.801677	8.04E-49	-1.69E-46
MS.gene058906	MS.gene35590	0.806071	9.83E-50	-1.69E-46
MS.gene058982	MS.gene35590	0.805733	1.16E-49	-1.69E-46
MS.gene059006	MS.gene35590	0.83868	2.49E-57	-1.69E-46
MS.gene059124	MS.gene35590	0.800698	1.28E-48	-1.69E-46
MS.gene060084	MS.gene35590	0.80393	2.76E-49	-1.69E-46
MS.gene060476	MS.gene35590	0.836406	9.53E-57	-1.69E-46
MS.gene061113	MS.gene35590	0.845369	4.24E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35590.t1	MTR_3g074500	97.436	78	2	0	1	78	1	78	4.94e-52	158
MS.gene35590.t1	MTR_3g074500	97.436	78	2	0	1	78	1	78	5.67e-52	158

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35590.t1	AT1G34640	61.111	72	28	0	1	72	1	72	1.99e-22	83.6

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGGTATTGTTGGAATTTC+TGG	0.095978	3.3:-64250968	MS.gene35590:CDS
GATCTTTCCTCAACAGTAAC+AGG	0.350655	3.3:+64250890	None:intergenic
TTGGCTAGCAGCAGTTATAT+TGG	0.391625	3.3:-64251041	MS.gene35590:CDS
ACCTATGTGTTGGGTATTGT+TGG	0.449040	3.3:-64250977	MS.gene35590:CDS
TCCTCAACAGTAACAGGATA+AGG	0.457912	3.3:+64250896	None:intergenic
ATGTTCGTTACCTATGTGTT+GGG	0.458889	3.3:-64250986	MS.gene35590:CDS
CTCTAAGAACCTCTATACTT+TGG	0.483285	3.3:-64251060	MS.gene35590:CDS
CGGTTGAAATAATCCCAATC+AGG	0.486481	3.3:+64250935	None:intergenic
GCAGCAGTTATATTGGTAGT+TGG	0.490292	3.3:-64251034	MS.gene35590:CDS
TCAGCAGCTTCTCTTTCTCA+TGG	0.490405	3.3:-64250869	MS.gene35590:CDS
GCCTTATCCTGTTACTGTTG+AGG	0.496290	3.3:-64250897	MS.gene35590:CDS
TGCTGCTAGCCAAAGTATAG+AGG	0.504355	3.3:+64251051	None:intergenic
GATGTTCGTTACCTATGTGT+TGG	0.512795	3.3:-64250987	MS.gene35590:CDS
TAGAGCTGCATCATTTGCCA+TGG	0.517008	3.3:+64251078	None:intergenic
TCAACCGTGATATTTCTCGT+TGG	0.602906	3.3:-64250919	MS.gene35590:CDS
TCCAACAATACCCAACACAT+AGG	0.663389	3.3:+64250976	None:intergenic
AAGGCCAACGAGAAATATCA+CGG	0.673257	3.3:+64250915	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGAATATAAATGAATTAA+GGG	+	chr3.3:64251051-64251070	None:intergenic	10.0%
!!	TAAAGAATATAAATGAATTA+AGG	+	chr3.3:64251052-64251071	None:intergenic	10.0%
	ATGTTCGTTACCTATGTGTT+GGG	-	chr3.3:64250887-64250906	MS.gene35590:CDS	35.0%
!	CTCTAAGAACCTCTATACTT+TGG	-	chr3.3:64250813-64250832	MS.gene35590:intron	35.0%
!	TTGGGTATTGTTGGAATTTC+TGG	-	chr3.3:64250905-64250924	MS.gene35590:CDS	35.0%
	AAGGCCAACGAGAAATATCA+CGG	+	chr3.3:64250961-64250980	None:intergenic	40.0%
	CGGTTGAAATAATCCCAATC+AGG	+	chr3.3:64250941-64250960	None:intergenic	40.0%
	GATCTTTCCTCAACAGTAAC+AGG	+	chr3.3:64250986-64251005	None:intergenic	40.0%
	GATGTTCGTTACCTATGTGT+TGG	-	chr3.3:64250886-64250905	MS.gene35590:CDS	40.0%
	TCAACCGTGATATTTCTCGT+TGG	-	chr3.3:64250954-64250973	MS.gene35590:CDS	40.0%
	TCCAACAATACCCAACACAT+AGG	+	chr3.3:64250900-64250919	None:intergenic	40.0%
	TCCTCAACAGTAACAGGATA+AGG	+	chr3.3:64250980-64250999	None:intergenic	40.0%
	TTGGCTAGCAGCAGTTATAT+TGG	-	chr3.3:64250832-64250851	MS.gene35590:intron	40.0%
!	ACCTATGTGTTGGGTATTGT+TGG	-	chr3.3:64250896-64250915	MS.gene35590:CDS	40.0%
!	GCAGCAGTTATATTGGTAGT+TGG	-	chr3.3:64250839-64250858	MS.gene35590:intron	40.0%
!	TGGTGTTCTTTTGCCTGATT+GGG	-	chr3.3:64250925-64250944	MS.gene35590:CDS	40.0%
	GCCTTATCCTGTTACTGTTG+AGG	-	chr3.3:64250976-64250995	MS.gene35590:CDS	45.0%
	TCAGCAGCTTCTCTTTCTCA+TGG	-	chr3.3:64251004-64251023	MS.gene35590:CDS	45.0%
	TGCTGCTAGCCAAAGTATAG+AGG	+	chr3.3:64250825-64250844	None:intergenic	45.0%
!	CTGGTGTTCTTTTGCCTGAT+TGG	-	chr3.3:64250924-64250943	MS.gene35590:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	64250797	64251098	64250797	ID=MS.gene35590
chr3.3	mRNA	64250797	64251098	64250797	ID=MS.gene35590.t1;Parent=MS.gene35590
chr3.3	exon	64250865	64251098	64250865	ID=MS.gene35590.t1.exon1;Parent=MS.gene35590.t1
chr3.3	CDS	64250865	64251098	64250865	ID=cds.MS.gene35590.t1;Parent=MS.gene35590.t1
chr3.3	exon	64250797	64250808	64250797	ID=MS.gene35590.t1.exon2;Parent=MS.gene35590.t1
chr3.3	CDS	64250797	64250808	64250797	ID=cds.MS.gene35590.t1;Parent=MS.gene35590.t1

Gene Sequence	>MS.gene35590 ATGGCAAATGATGCAGCTCTAAGAACCTCTATACTTTGGCTAGCAGCAGTTATATTGGTAGTTGGTATATGCACATACTCTTTCAAGAAGATGTTCGTTACCTATGTGTTGGGTATTGTTGGAATTTCTGGTGTTCTTTTGCCTGATTGGGATTATTTCAACCGTGATATTTCTCGTTGGCCTTATCCTGTTACTGTTGAGGAAAGATCAGCAGCTTCTCTTTCTCATGGATCTGTATTTCTAAAGTGAGTCCCTTAATTCATTTATATTCTTTATATATACTCTTGTAGACTAGATTTTGA
Protein sequence	>MS.gene35590.t1 MANDAALRTSILWLAAVILVVGICTYSFKKMFVTYVLGIVGISGVLLPDWDYFNRDISRWPYPVTVEERSAASLSHGSTRF