AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36046


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36046.t1 MTR_8g058785 97.674 129 3 0 1 129 1 129 2.66e-91 260
MS.gene36046.t1 MTR_8g061270 97.674 129 3 0 1 129 1 129 2.66e-91 260
MS.gene36046.t1 MTR_2g078670 71.094 128 37 0 1 128 1 128 6.64e-64 191
MS.gene36046.t1 MTR_4g088505 57.031 128 53 1 1 128 1 126 1.78e-48 152
MS.gene36046.t1 MTR_3g070330 52.344 128 61 0 1 128 24 151 6.35e-46 146
MS.gene36046.t1 MTR_3g070330 60.194 103 41 0 1 103 24 126 6.49e-45 142
MS.gene36046.t1 MTR_7g068250 57.273 110 47 0 1 110 1 110 2.40e-44 141
MS.gene36046.t1 MTR_5g082490 59.091 110 45 0 1 110 1 110 6.54e-44 140
MS.gene36046.t1 MTR_4g088505 61.446 83 32 0 1 83 1 83 1.32e-33 114
MS.gene36046.t1 MTR_3g091840 47.423 97 51 0 12 108 8 104 2.26e-31 107
MS.gene36046.t1 MTR_8g027255 44.211 95 53 0 12 106 8 102 2.40e-26 95.1
MS.gene36046.t1 MTR_1g077750 44.048 84 47 0 12 95 8 91 6.35e-26 94.0
MS.gene36046.t1 MTR_1g077760 42.593 54 31 0 48 101 1 54 5.25e-13 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36046.t1 AT5G53940 69.531 128 39 0 1 128 1 128 8.47e-62 185
MS.gene36046.t1 AT2G40110 55.814 129 56 1 1 128 1 129 4.28e-47 148
MS.gene36046.t1 AT3G08990 51.562 128 62 0 1 128 1 128 1.43e-46 147
MS.gene36046.t1 AT3G08990 51.562 128 62 0 1 128 1 128 1.43e-46 147
MS.gene36046.t1 AT3G08990 51.562 128 62 0 1 128 1 128 1.43e-46 147
MS.gene36046.t1 AT2G40110 64.706 102 36 0 1 102 1 102 1.66e-46 146
MS.gene36046.t1 AT3G55890 58.095 105 44 0 1 105 1 105 6.83e-42 135
MS.gene36046.t1 AT3G55890 58.095 105 44 0 1 105 1 105 6.83e-42 135
MS.gene36046.t1 AT3G55890 59.406 101 41 0 1 101 1 101 1.96e-41 133
MS.gene36046.t1 AT3G55890 59.406 101 41 0 1 101 1 101 1.96e-41 133
MS.gene36046.t1 AT3G11230 50.781 128 63 0 1 128 1 128 4.25e-41 133
MS.gene36046.t1 AT3G11230 50.781 128 63 0 1 128 34 161 1.70e-40 132
MS.gene36046.t1 AT3G11230 57.843 102 43 0 1 102 1 102 1.46e-39 128
MS.gene36046.t1 AT3G11230 57.843 102 43 0 1 102 34 135 2.62e-39 129
MS.gene36046.t1 AT2G40110 61.446 83 32 0 1 83 1 83 2.08e-35 117
MS.gene36046.t1 AT2G40110 61.446 83 32 0 1 83 1 83 2.08e-35 117
MS.gene36046.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.48e-31 107
MS.gene36046.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.48e-31 107
MS.gene36046.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.48e-31 107
MS.gene36046.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.48e-31 107
MS.gene36046.t1 AT4G27740 39.130 92 56 0 14 105 10 101 6.26e-23 86.7

Find 22 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAAAGAGCGATGCTTTC+AGG 0.190195 8.1:-52552657 MS.gene36046:CDS
ACTTTCCCTCTTTGTACTTT+TGG 0.194241 8.1:+52552422 None:intergenic
TCCACTGAATTCTGTGTTGA+AGG 0.319590 8.1:-52552259 MS.gene36046:CDS
GAGCTGGATGGCAGAGTTTA+CGG 0.331875 8.1:-52553479 MS.gene36046:CDS
TGTCGCTGCGGTCAGATAAT+TGG 0.336845 8.1:-52552606 MS.gene36046:CDS
GGAAGGATCGTTGATGAAAT+CGG 0.431542 8.1:-52552286 MS.gene36046:CDS
ATACTTACACATTATTGAAA+AGG 0.455887 8.1:+52553274 None:intergenic
TTTCCACTGATCTCATCTCC+AGG 0.460967 8.1:-52553427 MS.gene36046:CDS
GAAAAGCCAAAAGTACAAAG+AGG 0.465104 8.1:-52552428 MS.gene36046:CDS
AGGGAAAGTTTGTTCTTGAA+AGG 0.474035 8.1:-52552408 MS.gene36046:intron
TGGAAAGAGCGATGCTTTCA+GGG 0.476055 8.1:-52552656 MS.gene36046:CDS
AAAAGCCAAAAGTACAAAGA+GGG 0.574439 8.1:-52552427 MS.gene36046:CDS
TTCCACTGATCTCATCTCCA+GGG 0.580481 8.1:-52553426 MS.gene36046:intron
GAGTGATACTGAAGATGAGT+AGG 0.584188 8.1:-52552224 None:intergenic
GCTGCGGTCAGATAATTGGC+TGG 0.590004 8.1:-52552602 MS.gene36046:CDS
CACAGTGTGAACTTCACTAG+TGG 0.591434 8.1:-52552687 MS.gene36046:intron
CTTCACTAGTGGAGAAGTGG+TGG 0.595583 8.1:-52552676 MS.gene36046:CDS
TCTCTCATAGGAAACTGTGC+AGG 0.598241 8.1:-52552452 MS.gene36046:intron
TGAGATCAGTGGAAAGAGCA+AGG 0.604806 8.1:+52553435 None:intergenic
GCCTTCAACACAGAATTCAG+TGG 0.633670 8.1:+52552258 None:intergenic
TACCCTGGAGATGAGATCAG+TGG 0.654916 8.1:+52553424 None:intergenic
GAACTTCACTAGTGGAGAAG+TGG 0.657506 8.1:-52552679 MS.gene36046:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACAATCTAATTCTAATAT+CGG + chr8.1:52552933-52552952 None:intergenic 15.0%
!!! TTCAATTTTTTTGGTTAATT+AGG - chr8.1:52552346-52552365 MS.gene36046:intron 15.0%
!! AATTGTATGTTAAGTTATAC+TGG - chr8.1:52552727-52552746 MS.gene36046:intron 20.0%
!! ATACTTACACATTATTGAAA+AGG + chr8.1:52552454-52552473 None:intergenic 20.0%
!! ATTGTATGTTAAGTTATACT+GGG - chr8.1:52552728-52552747 MS.gene36046:intron 20.0%
!! TCAATAATGGTATTATAGTA+AGG - chr8.1:52552554-52552573 MS.gene36046:intron 20.0%
!! TTAACCAAAAAAATTGAAGA+AGG + chr8.1:52552344-52552363 None:intergenic 20.0%
!!! AATGCAATTTTATTTTCAAC+TGG + chr8.1:52552610-52552629 None:intergenic 20.0%
!!! GACAGTTTTATTATCAATAA+TGG - chr8.1:52552541-52552560 MS.gene36046:intron 20.0%
! AAACAATAAGGGTTAATGAA+TGG + chr8.1:52552388-52552407 None:intergenic 25.0%
! AGAAGAAAAGCAAACAATAA+GGG + chr8.1:52552399-52552418 None:intergenic 25.0%
! AGATAAGTATTTCAACATTC+AGG + chr8.1:52552854-52552873 None:intergenic 25.0%
! ATCAGTAAAAACACACATTA+AGG + chr8.1:52553389-52553408 None:intergenic 25.0%
! GATAAGTATTTCAACATTCA+GGG + chr8.1:52552853-52552872 None:intergenic 25.0%
! TTTGTAAAAATAAGAGAGGA+TGG + chr8.1:52553167-52553186 None:intergenic 25.0%
!! TCGATTTGTAAAAATAAGAG+AGG + chr8.1:52553171-52553190 None:intergenic 25.0%
!!! CATTTAAGATTTGTTACTGA+TGG + chr8.1:52552504-52552523 None:intergenic 25.0%
!!! TGATCCTTCTTCAATTTTTT+TGG - chr8.1:52552337-52552356 MS.gene36046:intron 25.0%
GAGAAGAAAAGCAAACAATA+AGG + chr8.1:52552400-52552419 None:intergenic 30.0%
TAAAATTGCATCTCTCTCAT+AGG - chr8.1:52553261-52553280 MS.gene36046:intron 30.0%
! AAAAGCCAAAAGTACAAAGA+GGG - chr8.1:52553298-52553317 MS.gene36046:CDS 30.0%
! TTTAGAAGGATCAAAAGTAC+TGG + chr8.1:52553245-52553264 None:intergenic 30.0%
!! ATTGTTTGCTTTTCTTCTCA+GGG - chr8.1:52552401-52552420 MS.gene36046:intron 30.0%
!! TATTGTTTGCTTTTCTTCTC+AGG - chr8.1:52552400-52552419 MS.gene36046:intron 30.0%
!! TTGTTACTGATGGATTAACA+AGG + chr8.1:52552494-52552513 None:intergenic 30.0%
CATGATATGATATGATACCC+TGG + chr8.1:52552319-52552338 None:intergenic 35.0%
CTCAACAAATTGAAATGCCT+CGG + chr8.1:52552696-52552715 None:intergenic 35.0%
GTAAAAATAAGAGAGGATGG+TGG + chr8.1:52553164-52553183 None:intergenic 35.0%
TATACTGGGATTGTTGTAAC+TGG - chr8.1:52552742-52552761 MS.gene36046:intron 35.0%
! ACTTTCCCTCTTTGTACTTT+TGG + chr8.1:52553306-52553325 None:intergenic 35.0%
! AGGGAAAGTTTGTTCTTGAA+AGG - chr8.1:52553317-52553336 MS.gene36046:intron 35.0%
! AGTTTTCATGGTTGAATGGT+GGG - chr8.1:52552775-52552794 MS.gene36046:intron 35.0%
! CAGGGATTTTATTGTCATAG+AGG - chr8.1:52552419-52552438 MS.gene36046:CDS 35.0%
! GAAAAGCCAAAAGTACAAAG+AGG - chr8.1:52553297-52553316 MS.gene36046:CDS 35.0%
! GAGAGAGATGCAATTTTAGA+AGG + chr8.1:52553259-52553278 None:intergenic 35.0%
! TGTCAGTTTTCATGGTTGAA+TGG - chr8.1:52552771-52552790 MS.gene36046:intron 35.0%
!! GATATATTTTGTTGTCGCTG+CGG - chr8.1:52553107-52553126 MS.gene36046:intron 35.0%
ACAAAACTCCCACATACGTA+AGG + chr8.1:52552814-52552833 None:intergenic 40.0%
ACGATCCTTCCTCTGTTAAA+TGG + chr8.1:52553430-52553449 None:intergenic 40.0%
CATGCTTGTCCATTTAACAG+AGG - chr8.1:52553418-52553437 MS.gene36046:intron 40.0%
CTAATACCAAACTAATGCCG+AGG - chr8.1:52552676-52552695 MS.gene36046:CDS 40.0%
CTTGTCCATTTAACAGAGGA+AGG - chr8.1:52553422-52553441 MS.gene36046:intron 40.0%
TCCACTGAATTCTGTGTTGA+AGG - chr8.1:52553466-52553485 MS.gene36046:CDS 40.0%
! ATTTTTGCAGTTGAGCTGGA+TGG - chr8.1:52552234-52552253 MS.gene36046:CDS 40.0%
! CAGTTTTCATGGTTGAATGG+TGG - chr8.1:52552774-52552793 MS.gene36046:intron 40.0%
! CATGTGCATGCTTGTGTTTT+TGG - chr8.1:52553008-52553027 MS.gene36046:intron 40.0%
! GAGAATTTTTGCAGTTGAGC+TGG - chr8.1:52552230-52552249 MS.gene36046:CDS 40.0%
! GGAAGGATCGTTGATGAAAT+CGG - chr8.1:52553439-52553458 MS.gene36046:CDS 40.0%
!! TATGTGGGAGTTTTGTACAC+CGG - chr8.1:52552818-52552837 MS.gene36046:intron 40.0%
ACATACGTAAGGTCCTGGAA+AGG + chr8.1:52552803-52552822 None:intergenic 45.0%
CACAGTGTGAACTTCACTAG+TGG - chr8.1:52553038-52553057 MS.gene36046:intron 45.0%
CATACGTAAGGTCCTGGAAA+GGG + chr8.1:52552802-52552821 None:intergenic 45.0%
GAAATGCCTCGGCATTAGTT+TGG + chr8.1:52552685-52552704 None:intergenic 45.0%
GAACTTCACTAGTGGAGAAG+TGG - chr8.1:52553046-52553065 MS.gene36046:intron 45.0%
GCCTTCAACACAGAATTCAG+TGG + chr8.1:52553470-52553489 None:intergenic 45.0%
GTGAAGTTCACACTGTGTCA+TGG + chr8.1:52553035-52553054 None:intergenic 45.0%
TCTCTCATAGGAAACTGTGC+AGG - chr8.1:52553273-52553292 MS.gene36046:intron 45.0%
TGAGATCAGTGGAAAGAGCA+AGG + chr8.1:52552293-52552312 None:intergenic 45.0%
TGGAAAGAGCGATGCTTTCA+GGG - chr8.1:52553069-52553088 MS.gene36046:intron 45.0%
TTCCACTGATCTCATCTCCA+GGG - chr8.1:52552299-52552318 MS.gene36046:intron 45.0%
TTCCAGGACCTTACGTATGT+GGG - chr8.1:52552803-52552822 MS.gene36046:intron 45.0%
TTTCCACTGATCTCATCTCC+AGG - chr8.1:52552298-52552317 MS.gene36046:intron 45.0%
TTTCCAGGACCTTACGTATG+TGG - chr8.1:52552802-52552821 MS.gene36046:intron 45.0%
! AAGAGCAAGGTGAGTTTTGC+AGG + chr8.1:52552280-52552299 None:intergenic 45.0%
! ATTCTGCGTCTGTGTTGATG+TGG - chr8.1:52552627-52552646 MS.gene36046:CDS 45.0%
!!! GGCTAGCTTGTCAGTTTTCA+TGG - chr8.1:52552763-52552782 MS.gene36046:intron 45.0%
CTTCACTAGTGGAGAAGTGG+TGG - chr8.1:52553049-52553068 MS.gene36046:intron 50.0%
GAGCTGGATGGCAGAGTTTA+CGG - chr8.1:52552246-52552265 MS.gene36046:CDS 50.0%
GTGGAAAGAGCGATGCTTTC+AGG - chr8.1:52553068-52553087 MS.gene36046:intron 50.0%
TACCCTGGAGATGAGATCAG+TGG + chr8.1:52552304-52552323 None:intergenic 50.0%
TGTCGCTGCGGTCAGATAAT+TGG - chr8.1:52553119-52553138 MS.gene36046:intron 50.0%
!! TGGGAGTTTTGTACACCGGA+CGG - chr8.1:52552822-52552841 MS.gene36046:intron 50.0%
CTCCCACATACGTAAGGTCC+TGG + chr8.1:52552808-52552827 None:intergenic 55.0%
GCTGCGGTCAGATAATTGGC+TGG - chr8.1:52553123-52553142 MS.gene36046:intron 55.0%
! TGAATGGTGGGACCCTTTCC+AGG - chr8.1:52552787-52552806 MS.gene36046:intron 55.0%
!! GGGAGTTTTGTACACCGGAC+GGG - chr8.1:52552823-52552842 MS.gene36046:intron 55.0%
ACATTCAGGGCAGTCCCGTC+CGG + chr8.1:52552840-52552859 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 52552225 52553522 52552225 ID=MS.gene36046
chr8.1 mRNA 52552225 52553522 52552225 ID=MS.gene36046.t1;Parent=MS.gene36046
chr8.1 exon 52553427 52553522 52553427 ID=MS.gene36046.t1.exon1;Parent=MS.gene36046.t1
chr8.1 CDS 52553427 52553522 52553427 ID=cds.MS.gene36046.t1;Parent=MS.gene36046.t1
chr8.1 exon 52553282 52553325 52553282 ID=MS.gene36046.t1.exon2;Parent=MS.gene36046.t1
chr8.1 CDS 52553282 52553325 52553282 ID=cds.MS.gene36046.t1;Parent=MS.gene36046.t1
chr8.1 exon 52552596 52552704 52552596 ID=MS.gene36046.t1.exon3;Parent=MS.gene36046.t1
chr8.1 CDS 52552596 52552704 52552596 ID=cds.MS.gene36046.t1;Parent=MS.gene36046.t1
chr8.1 exon 52552409 52552464 52552409 ID=MS.gene36046.t1.exon4;Parent=MS.gene36046.t1
chr8.1 CDS 52552409 52552464 52552409 ID=cds.MS.gene36046.t1;Parent=MS.gene36046.t1
chr8.1 exon 52552225 52552309 52552225 ID=MS.gene36046.t1.exon5;Parent=MS.gene36046.t1
chr8.1 CDS 52552225 52552309 52552225 ID=cds.MS.gene36046.t1;Parent=MS.gene36046.t1
Gene Sequence

>MS.gene36046

ATGGGGAGAATTTTTGCAGTTGAGCTGGATGGCAGAGTTTACGGCTGCAAGTCCTGCAAAACTCACCTTGCTCTTTCCACTGATCTCATCTCCAGGGTATCATATCATATCATGATCCTTCTTCAATTTTTTTGGTTAATTAGGTGTTTCAACTAATTAACCATTCATTAACCCTTATTGTTTGCTTTTCTTCTCAGGGATTTTATTGTCATAGAGGAAAAGCATACCTTTTCAATAATGTGTAAGTATTTTTCATTTTTTGTTTTCCTTGTTAATCCATCAGTAACAAATCTTAAATGAATTACTACAAAGTTATGACAGTTTTATTATCAATAATGGTATTATAGTAAGGCTTATATCATTTATTATTGTTCTCACTTTTCCAGTTGAAAATAAAATTGCATTCTGCGTCTGTGTTGATGTGGAAAATGTATTAGTGTGTTTCTAATTTCTAATACCAAACTAATGCCGAGGCATTTCAATTTGTTGAGCTACATATAAGAATTGTATGTTAAGTTATACTGGGATTGTTGTAACTGGCTAGCTTGTCAGTTTTCATGGTTGAATGGTGGGACCCTTTCCAGGACCTTACGTATGTGGGAGTTTTGTACACCGGACGGGACTGCCCTGAATGTTGAAATACTTATCTTATGCTTTGATTAGATTATTATGTATAATTGTACATGCTTCAACATCTTTGACATACCGATATTAGAATTAGATTGTATTGATTGAAATTATAAATACAAACTAGATCTATGCTTTTTTATTATTAACAATTCTCATGTGCATGCTTGTGTTTTTGGTCCATGACACAGTGTGAACTTCACTAGTGGAGAAGTGGTGGAAAGAGCGATGCTTTCAGGGATGCATACTGTCGCAGATATATTTTGTTGTCGCTGCGGTCAGATAATTGGCTGGAAATACGTAATTCTTCCACCATCCTCTCTTATTTTTACAAATCGATATCTTCAAACTAGTGTTGTAGATGAAATCTATTTGAATATCATTCTGCATCCAGTACTTTTGATCCTTCTAAAATTGCATCTCTCTCATAGGAAACTGTGCAGGAGAAAAGCCAAAAGTACAAAGAGGGAAAGTTTGTTCTTGAAAGGTATTATGCTATTTTGCTATCACAACTTGAGTTGAGTTTTTTCTTATCCTTAATGTGTGTTTTTACTGATTGTTCATGTCATGCTTGTCCATTTAACAGAGGAAGGATCGTTGATGAAATCGGTTTCTCCACTGAATTCTGTGTTGAAGGCTGTGTCAGCATGAGTGATACTGAAGATGAGTAG

Protein sequence

>MS.gene36046.t1

MGRIFAVELDGRVYGCKSCKTHLALSTDLISRGFYCHRGKAYLFNNVVNFTSGEVVERAMLSGMHTVADIFCCRCGQIIGWKYETVQEKSQKYKEGKFVLERGRIVDEIGFSTEFCVEGCVSMSDTEDE