Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38689.t1 | XP_013461718.1 | 95.8 | 190 | 8 | 0 | 1 | 190 | 1 | 190 | 1.80E-94 | 355.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38689.t1 | Q9C642 | 50.0 | 108 | 54 | 0 | 84 | 191 | 99 | 206 | 1.8e-26 | 121.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38689.t1 | A0A072V322 | 95.8 | 190 | 8 | 0 | 1 | 190 | 1 | 190 | 1.3e-94 | 355.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050301 | MS.gene38689 | 0.803536 | 3.33E-49 | -1.69E-46 |
| MS.gene050404 | MS.gene38689 | 0.806474 | 8.08E-50 | -1.69E-46 |
| MS.gene050406 | MS.gene38689 | 0.801581 | 8.41E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene38689 | 0.829279 | 5.63E-55 | -1.69E-46 |
| MS.gene051171 | MS.gene38689 | 0.861273 | 1.15E-63 | -1.69E-46 |
| MS.gene051240 | MS.gene38689 | 0.848892 | 4.59E-60 | -1.69E-46 |
| MS.gene051242 | MS.gene38689 | 0.863133 | 3.09E-64 | -1.69E-46 |
| MS.gene051244 | MS.gene38689 | 0.828414 | 9.11E-55 | -1.69E-46 |
| MS.gene051582 | MS.gene38689 | 0.833428 | 5.36E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene38689 | 0.807004 | 6.25E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene38689 | -0.810719 | 1.00E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene38689 | 0.806064 | 9.86E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene38689 | 0.802045 | 6.76E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene38689 | 0.808722 | 2.69E-50 | -1.69E-46 |
| MS.gene052600 | MS.gene38689 | 0.824223 | 9.07E-54 | -1.69E-46 |
| MS.gene053330 | MS.gene38689 | 0.840125 | 1.05E-57 | -1.69E-46 |
| MS.gene053836 | MS.gene38689 | 0.837702 | 4.45E-57 | -1.69E-46 |
| MS.gene053907 | MS.gene38689 | 0.839021 | 2.03E-57 | -1.69E-46 |
| MS.gene053971 | MS.gene38689 | 0.825562 | 4.38E-54 | -1.69E-46 |
| MS.gene054942 | MS.gene38689 | 0.80074 | 1.25E-48 | -1.69E-46 |
| MS.gene054983 | MS.gene38689 | 0.807066 | 6.06E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene38689 | 0.837654 | 4.58E-57 | -1.69E-46 |
| MS.gene05553 | MS.gene38689 | 0.846657 | 1.89E-59 | -1.69E-46 |
| MS.gene055971 | MS.gene38689 | 0.837145 | 6.18E-57 | -1.69E-46 |
| MS.gene056305 | MS.gene38689 | 0.807691 | 4.47E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene38689 | 0.823663 | 1.23E-53 | -1.69E-46 |
| MS.gene056633 | MS.gene38689 | 0.819666 | 1.03E-52 | -1.69E-46 |
| MS.gene056664 | MS.gene38689 | 0.851894 | 6.59E-61 | -1.69E-46 |
| MS.gene056796 | MS.gene38689 | 0.810259 | 1.26E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene38689 | 0.800794 | 1.22E-48 | -1.69E-46 |
| MS.gene05702 | MS.gene38689 | 0.808261 | 3.38E-50 | -1.69E-46 |
| MS.gene057121 | MS.gene38689 | 0.828735 | 7.62E-55 | -1.69E-46 |
| MS.gene058002 | MS.gene38689 | 0.82556 | 4.39E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene38689 | 0.827632 | 1.41E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene38689 | 0.838286 | 3.15E-57 | -1.69E-46 |
| MS.gene058233 | MS.gene38689 | 0.857004 | 2.19E-62 | -1.69E-46 |
| MS.gene058276 | MS.gene38689 | 0.883124 | 5.85E-71 | -1.69E-46 |
| MS.gene058365 | MS.gene38689 | 0.812423 | 4.27E-51 | -1.69E-46 |
| MS.gene058746 | MS.gene38689 | 0.85531 | 6.87E-62 | -1.69E-46 |
| MS.gene05907 | MS.gene38689 | 0.804342 | 2.26E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene38689 | 0.816398 | 5.64E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene38689 | 0.838375 | 2.99E-57 | -1.69E-46 |
| MS.gene060407 | MS.gene38689 | 0.839352 | 1.67E-57 | -1.69E-46 |
| MS.gene061049 | MS.gene38689 | 0.825605 | 4.28E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38689.t1 | MTR_3g102270 | 96.842 | 190 | 6 | 0 | 1 | 190 | 1 | 190 | 1.00e-139 | 392 |
| MS.gene38689.t1 | MTR_3g102270 | 86.184 | 152 | 20 | 1 | 16 | 167 | 16 | 166 | 6.08e-93 | 273 |
| MS.gene38689.t1 | MTR_3g102270 | 94.074 | 135 | 8 | 0 | 16 | 150 | 16 | 150 | 2.23e-92 | 271 |
| MS.gene38689.t1 | MTR_3g101770 | 50.450 | 111 | 55 | 0 | 81 | 191 | 101 | 211 | 2.73e-35 | 127 |
| MS.gene38689.t1 | MTR_8g038990 | 43.363 | 113 | 64 | 0 | 79 | 191 | 1815 | 1927 | 1.27e-25 | 107 |
| MS.gene38689.t1 | MTR_3g064080 | 91.111 | 45 | 4 | 0 | 248 | 292 | 84 | 128 | 2.38e-20 | 91.3 |
| MS.gene38689.t1 | MTR_5g011000 | 36.207 | 116 | 66 | 4 | 67 | 177 | 1333 | 1445 | 1.72e-13 | 71.2 |
| MS.gene38689.t1 | MTR_3g064090 | 46.154 | 65 | 34 | 1 | 228 | 292 | 68 | 131 | 1.44e-12 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38689.t1 | AT3G46630 | 57.377 | 183 | 65 | 5 | 17 | 190 | 17 | 195 | 1.59e-61 | 194 |
| MS.gene38689.t1 | AT1G45230 | 48.649 | 111 | 57 | 0 | 81 | 191 | 96 | 206 | 5.44e-32 | 118 |
| MS.gene38689.t1 | AT1G45230 | 48.649 | 111 | 57 | 0 | 81 | 191 | 96 | 206 | 1.55e-31 | 117 |
| MS.gene38689.t1 | AT2G40030 | 46.226 | 106 | 57 | 0 | 85 | 190 | 1735 | 1840 | 5.05e-27 | 111 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACACCTTGAGTGTCGAAAT+TGG | 0.273641 | 3.3:-84565214 | None:intergenic |
| CCGATTTAACGTGTTGTTAA+CGG | 0.274705 | 3.3:-84552831 | None:intergenic |
| AAGAATCTGATTATCGAAAA+TGG | 0.276535 | 3.3:+84552894 | MS.gene38689:CDS |
| GCTATAAGTGCTTACCATAA+TGG | 0.279775 | 3.3:-84553181 | None:intergenic |
| TTGCGGCGCGCCATCGTTCA+TGG | 0.324166 | 3.3:+84552753 | MS.gene38689:CDS |
| TCCTTAGTAAGCACGATAAT+AGG | 0.329251 | 3.3:-84552953 | None:intergenic |
| CGGTGAAGTGTTTGTAGTTA+TGG | 0.331589 | 3.3:-84552721 | None:intergenic |
| AGTAGAGTGGTGGGTGGCTC+TGG | 0.332385 | 3.3:+84567543 | MS.gene38689:CDS |
| ATTTATGATGGTTGCTTCCT+TGG | 0.335172 | 3.3:+84569098 | MS.gene38689:CDS |
| TGGCCGCACCACTTCTTCTA+AGG | 0.356825 | 3.3:+84552672 | MS.gene38689:CDS |
| TTTATTAATAGTACAAATAT+CGG | 0.375443 | 3.3:+84567394 | MS.gene38689:intron |
| ACACCTTGAGTGTCGAAATT+GGG | 0.379682 | 3.3:-84565213 | None:intergenic |
| TTTATGATGGTTGCTTCCTT+GGG | 0.395594 | 3.3:+84569099 | MS.gene38689:CDS |
| TGCGTCGGCGACGGCGAGGC+AGG | 0.399079 | 3.3:-84569033 | None:intergenic |
| TGGTGGGTGGCTCTGGTGAT+AGG | 0.399264 | 3.3:+84567550 | MS.gene38689:CDS |
| ATGTGGTCTTGAATCCATTA+TGG | 0.401081 | 3.3:+84553167 | MS.gene38689:CDS |
| ACCTATTATCGTGCTTACTA+AGG | 0.426424 | 3.3:+84552952 | MS.gene38689:CDS |
| CCCGTCGAGTAACTTCCGTT+TGG | 0.427801 | 3.3:+84552865 | MS.gene38689:CDS |
| CAAATTCGAAATTGCTCCGC+TGG | 0.440058 | 3.3:+84569056 | MS.gene38689:CDS |
| AACGTGTTGTTAACGGAGTT+AGG | 0.441542 | 3.3:-84552824 | None:intergenic |
| ACGGAGTTAGGTTTATCGTT+TGG | 0.446191 | 3.3:-84552812 | None:intergenic |
| GTCCAAGCAGAGGGTTCAGC+AGG | 0.456723 | 3.3:+84567472 | MS.gene38689:CDS |
| GTCGAGTAACTTCCGTTTGG+AGG | 0.460444 | 3.3:+84552868 | MS.gene38689:CDS |
| CCACACTCTGAAGATAAGAT+TGG | 0.461224 | 3.3:+84553144 | MS.gene38689:CDS |
| GGATGGAGGGACATTAACTT+TGG | 0.461982 | 3.3:+84553080 | MS.gene38689:CDS |
| CCGTTAACAACACGTTAAAT+CGG | 0.462228 | 3.3:+84552831 | MS.gene38689:CDS |
| GGTGCCCGTGTTATTTATGA+TGG | 0.462782 | 3.3:+84569086 | MS.gene38689:CDS |
| GGCCTGCTGAACCCTCTGCT+TGG | 0.465526 | 3.3:-84567474 | None:intergenic |
| TTGTAAGAACTGATGGTGGC+TGG | 0.484149 | 3.3:+84565243 | MS.gene38689:CDS |
| TCGAATTTGTGCGTCGGCGA+CGG | 0.485657 | 3.3:-84569042 | None:intergenic |
| GGTTCAGAACAAAGGGAAGA+TGG | 0.491438 | 3.3:+84567436 | MS.gene38689:CDS |
| GTCGGCGACGGCGAGGCAGG+CGG | 0.492929 | 3.3:-84569030 | None:intergenic |
| AATTGCTCCGCTGGTTCTGA+CGG | 0.525438 | 3.3:+84569065 | MS.gene38689:CDS |
| ATAAACCTTTCAGCATGAGC+TGG | 0.527085 | 3.3:-84565317 | None:intergenic |
| CAGATTCTTCATCCTCCAAA+CGG | 0.535477 | 3.3:-84552880 | None:intergenic |
| TGGAGGGACATTAACTTTGG+AGG | 0.540847 | 3.3:+84553083 | MS.gene38689:CDS |
| GCTGAACGGTTCAGAACAAA+GGG | 0.541271 | 3.3:+84567429 | MS.gene38689:CDS |
| CGGCGCTGCACCATGAACGA+TGG | 0.551636 | 3.3:-84552763 | None:intergenic |
| AGAGCTTGCCTTAGAAGAAG+TGG | 0.555098 | 3.3:-84552680 | None:intergenic |
| CACCTTGAGTGTCGAAATTG+GGG | 0.557551 | 3.3:-84565212 | None:intergenic |
| GGCTGAACGGTTCAGAACAA+AGG | 0.558120 | 3.3:+84567428 | MS.gene38689:CDS |
| CGACGGTGGATTGATGGCGG+CGG | 0.566544 | 3.3:-84552783 | None:intergenic |
| TGAACGATGGCGCGCCGCAA+CGG | 0.567387 | 3.3:-84552750 | None:intergenic |
| GCAGAGGGTTCAGCAGGCCG+CGG | 0.569078 | 3.3:+84567478 | MS.gene38689:CDS |
| GTCGTGAGGCAGTAGAGTGG+TGG | 0.574780 | 3.3:+84567533 | MS.gene38689:CDS |
| TAAACCTTTCAGCATGAGCT+GGG | 0.578041 | 3.3:-84565316 | None:intergenic |
| TTTGTTGTAAGAACTGATGG+TGG | 0.582232 | 3.3:+84565239 | MS.gene38689:CDS |
| TTTAAGGGCTCTGATACCCA+AGG | 0.582310 | 3.3:-84569115 | None:intergenic |
| CGCCGCCATCAATCCACCGT+CGG | 0.583751 | 3.3:+84552784 | MS.gene38689:CDS |
| CAAAGGGAAGATGGGTTGCA+CGG | 0.584178 | 3.3:+84567445 | MS.gene38689:CDS |
| GCGCGCCGCAACGGTTCTGA+CGG | 0.584424 | 3.3:-84552741 | None:intergenic |
| TTCCGACGGTGGATTGATGG+CGG | 0.592348 | 3.3:-84552786 | None:intergenic |
| CCAATCTTATCTTCAGAGTG+TGG | 0.595640 | 3.3:-84553144 | None:intergenic |
| TCGTGAGGCAGTAGAGTGGT+GGG | 0.596160 | 3.3:+84567534 | MS.gene38689:CDS |
| ATCTGATTATCGAAAATGGA+AGG | 0.596386 | 3.3:+84552898 | MS.gene38689:CDS |
| GTTCAGAACAAAGGGAAGAT+GGG | 0.597250 | 3.3:+84567437 | MS.gene38689:CDS |
| ATCCCCAATTTCGACACTCA+AGG | 0.598228 | 3.3:+84565210 | MS.gene38689:CDS |
| CCAAACGGAAGTTACTCGAC+GGG | 0.599011 | 3.3:-84552865 | None:intergenic |
| AGTGTCGAAATTGGGGATGA+CGG | 0.599976 | 3.3:-84565205 | None:intergenic |
| TCTGAAGATAAGATTGGATG+TGG | 0.603550 | 3.3:+84553150 | MS.gene38689:CDS |
| AGTACCCAGCTCATGCTGAA+AGG | 0.605237 | 3.3:+84565312 | MS.gene38689:CDS |
| GGAGGATGAGAAAGCTGTAG+TGG | 0.608022 | 3.3:+84553101 | MS.gene38689:CDS |
| TTTGTGCGTCGGCGACGGCG+AGG | 0.612790 | 3.3:-84569037 | None:intergenic |
| CAAATATCGGATGCACACAT+AGG | 0.613282 | 3.3:+84567407 | MS.gene38689:CDS |
| AGCAACCATCATAAATAACA+CGG | 0.614090 | 3.3:-84569091 | None:intergenic |
| ACGGGCACCGTCAGAACCAG+CGG | 0.624315 | 3.3:-84569072 | None:intergenic |
| TTGTGTGCTAGGTATATGGA+TGG | 0.625552 | 3.3:+84553063 | MS.gene38689:intron |
| GCAATTTCGAATTTGTGCGT+CGG | 0.626178 | 3.3:-84569048 | None:intergenic |
| CACGGAGGAGTCCAAGCAGA+GGG | 0.627683 | 3.3:+84567463 | MS.gene38689:CDS |
| CGCTTGTTGTTTCTCGACCG+CGG | 0.631592 | 3.3:-84567495 | None:intergenic |
| ACAAGCGAAAGACATGATAG+TGG | 0.640984 | 3.3:+84567511 | MS.gene38689:CDS |
| TCCAAACGGAAGTTACTCGA+CGG | 0.646116 | 3.3:-84552866 | None:intergenic |
| AAGACATGATAGTGGTCGTG+AGG | 0.657191 | 3.3:+84567519 | MS.gene38689:CDS |
| GGATGCACACATAGGCTGAA+CGG | 0.663240 | 3.3:+84567415 | MS.gene38689:CDS |
| TGTGCTAGGTATATGGATGG+AGG | 0.667189 | 3.3:+84553066 | MS.gene38689:intron |
| GCAACCATCATAAATAACAC+GGG | 0.671961 | 3.3:-84569090 | None:intergenic |
| GCACGGAGGAGTCCAAGCAG+AGG | 0.678534 | 3.3:+84567462 | MS.gene38689:CDS |
| GTGCTAGGTATATGGATGGA+GGG | 0.681449 | 3.3:+84553067 | MS.gene38689:intron |
| TGAGGCAGTAGAGTGGTGGG+TGG | 0.688099 | 3.3:+84567537 | MS.gene38689:CDS |
| TTGCCTTAGAAGAAGTGGTG+CGG | 0.691027 | 3.3:-84552675 | None:intergenic |
| GTGGTCGTGAGGCAGTAGAG+TGG | 0.693094 | 3.3:+84567530 | MS.gene38689:CDS |
| CTTCACCGTCAGAACCGTTG+CGG | 0.698015 | 3.3:+84552736 | MS.gene38689:CDS |
| AGGGAAGATGGGTTGCACGG+AGG | 0.732674 | 3.3:+84567448 | MS.gene38689:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 84552671 | 84569136 | 84552671 | ID=MS.gene38689 |
| chr3.3 | mRNA | 84552671 | 84569136 | 84552671 | ID=MS.gene38689.t1;Parent=MS.gene38689 |
| chr3.3 | exon | 84552671 | 84552993 | 84552671 | ID=MS.gene38689.t1.exon1;Parent=MS.gene38689.t1 |
| chr3.3 | CDS | 84552671 | 84552993 | 84552671 | ID=cds.MS.gene38689.t1;Parent=MS.gene38689.t1 |
| chr3.3 | exon | 84553074 | 84553188 | 84553074 | ID=MS.gene38689.t1.exon2;Parent=MS.gene38689.t1 |
| chr3.3 | CDS | 84553074 | 84553188 | 84553074 | ID=cds.MS.gene38689.t1;Parent=MS.gene38689.t1 |
| chr3.3 | exon | 84565200 | 84565333 | 84565200 | ID=MS.gene38689.t1.exon3;Parent=MS.gene38689.t1 |
| chr3.3 | CDS | 84565200 | 84565333 | 84565200 | ID=cds.MS.gene38689.t1;Parent=MS.gene38689.t1 |
| chr3.3 | exon | 84567405 | 84567576 | 84567405 | ID=MS.gene38689.t1.exon4;Parent=MS.gene38689.t1 |
| chr3.3 | CDS | 84567405 | 84567576 | 84567405 | ID=cds.MS.gene38689.t1;Parent=MS.gene38689.t1 |
| chr3.3 | exon | 84568999 | 84569136 | 84568999 | ID=MS.gene38689.t1.exon5;Parent=MS.gene38689.t1 |
| chr3.3 | CDS | 84568999 | 84569136 | 84568999 | ID=cds.MS.gene38689.t1;Parent=MS.gene38689.t1 |
| Gene Sequence |
| Protein sequence |
