AlfalfaGEDB | MS.gene44407

M. sativa cultivar XinJiangDaYe / MS.gene44407

KEGG: -
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00861.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44407.t1	XP_003592709.1	99.1	114	1	0	1	114	1	114	6.10E-51	209.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44407.t1	A8IAP8	45.7	92	49	1	21	112	29	119	5.9e-10	65.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44407.t1	G7IE75	99.1	114	1	0	1	114	1	114	4.4e-51	209.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049419	MS.gene44407	0.851083	1.12E-60	-1.69E-46
MS.gene050105	MS.gene44407	-0.848587	5.57E-60	-1.69E-46
MS.gene050654	MS.gene44407	-0.809209	2.12E-50	-1.69E-46
MS.gene051122	MS.gene44407	-0.848686	5.23E-60	-1.69E-46
MS.gene051275	MS.gene44407	-0.807278	5.47E-50	-1.69E-46
MS.gene051368	MS.gene44407	-0.810965	8.87E-51	-1.69E-46
MS.gene051793	MS.gene44407	0.818021	2.44E-52	-1.69E-46
MS.gene051828	MS.gene44407	0.811561	6.58E-51	-1.69E-46
MS.gene051924	MS.gene44407	0.818335	2.07E-52	-1.69E-46
MS.gene051926	MS.gene44407	0.823915	1.07E-53	-1.69E-46
MS.gene05216	MS.gene44407	-0.85056	1.57E-60	-1.69E-46
MS.gene052586	MS.gene44407	-0.805931	1.05E-49	-1.69E-46
MS.gene052674	MS.gene44407	-0.868695	5.39E-66	-1.69E-46
MS.gene052675	MS.gene44407	-0.869763	2.43E-66	-1.69E-46
MS.gene052677	MS.gene44407	-0.873561	1.34E-67	-1.69E-46
MS.gene054479	MS.gene44407	-0.848507	5.86E-60	-1.69E-46
MS.gene054950	MS.gene44407	0.815069	1.12E-51	-1.69E-46
MS.gene055063	MS.gene44407	0.813923	2.00E-51	-1.69E-46
MS.gene055327	MS.gene44407	-0.8002	1.61E-48	-1.69E-46
MS.gene055701	MS.gene44407	-0.842723	2.17E-58	-1.69E-46
MS.gene056045	MS.gene44407	-0.80623	9.10E-50	-1.69E-46
MS.gene056064	MS.gene44407	-0.829299	5.56E-55	-1.69E-46
MS.gene056068	MS.gene44407	-0.801484	8.81E-49	-1.69E-46
MS.gene056480	MS.gene44407	-0.847198	1.35E-59	-1.69E-46
MS.gene056779	MS.gene44407	-0.814649	1.38E-51	-1.69E-46
MS.gene057156	MS.gene44407	0.855419	6.39E-62	-1.69E-46
MS.gene058081	MS.gene44407	-0.839361	1.66E-57	-1.69E-46
MS.gene058223	MS.gene44407	0.871469	6.68E-67	-1.69E-46
MS.gene058429	MS.gene44407	-0.819941	8.92E-53	-1.69E-46
MS.gene059438	MS.gene44407	-0.804237	2.38E-49	-1.69E-46
MS.gene059439	MS.gene44407	-0.803996	2.67E-49	-1.69E-46
MS.gene059440	MS.gene44407	-0.818843	1.59E-52	-1.69E-46
MS.gene059952	MS.gene44407	-0.820482	6.70E-53	-1.69E-46
MS.gene06052	MS.gene44407	-0.813629	2.32E-51	-1.69E-46
MS.gene060850	MS.gene44407	-0.86765	1.17E-65	-1.69E-46
MS.gene060851	MS.gene44407	-0.88496	1.23E-71	-1.69E-46
MS.gene060852	MS.gene44407	-0.881711	1.91E-70	-1.69E-46
MS.gene060932	MS.gene44407	-0.822982	1.77E-53	-1.69E-46
MS.gene06121	MS.gene44407	-0.811435	7.01E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44407.t1	MTR_1g114200	99.123	114	1	1	114	1	114	2.37e-78	226
MS.gene44407.t1	MTR_4g032660	33.663	101	67	11	111	96	196	1.00e-16	72.4
MS.gene44407.t1	MTR_5g023360	70.833	48	14	1	48	46	93	2.11e-16	68.9
MS.gene44407.t1	MTR_3g012540	29.126	103	73	11	113	80	182	3.56e-13	62.8
MS.gene44407.t1	MTR_5g023330	75.000	32	8	1	32	88	119	2.47e-12	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44407.t1	AT5G27820	79.825	114	23	0	1	114	1	114	3.83e-65	192
MS.gene44407.t1	AT3G20230	35.644	101	65	0	11	111	83	183	6.97e-19	77.8
MS.gene44407.t1	AT3G45020	36.937	111	58	4	16	114	4	114	3.29e-13	61.6
MS.gene44407.t1	AT3G45020	36.937	111	58	4	16	114	4	114	3.29e-13	61.6
MS.gene44407.t1	AT3G45020	36.937	111	58	4	16	114	4	114	3.29e-13	61.6
MS.gene44407.t1	AT3G45020	36.937	111	58	4	16	114	4	114	3.29e-13	61.6
MS.gene44407.t1	AT1G08845	27.184	103	75	0	11	113	82	184	2.00e-12	60.8
MS.gene44407.t1	AT1G08845	27.184	103	75	0	11	113	82	184	2.00e-12	60.8
MS.gene44407.t1	AT1G08845	27.184	103	75	0	11	113	127	229	2.71e-12	61.2

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCAGATATTGTGTAAGTC+TGG	0.313396	1.1:-80279629	None:intergenic
CAACATCCCGAGTAGTTTCC+AGG	0.336475	1.1:-80279768	None:intergenic
CTGGGAGGCCTGACTGGAGG+TGG	0.388017	1.1:-80279610	None:intergenic
AAGCGGTTATTGATTCTCTC+AGG	0.400454	1.1:+80279899	MS.gene44407:CDS
GTAAGTCTGGGAGGCCTGAC+TGG	0.402204	1.1:-80279616	None:intergenic
AAATGCATCTTAAGAACATA+AGG	0.422008	1.1:-80279652	None:intergenic
GAAAGCCTTGAGATCAAGCC+TGG	0.444737	1.1:+80279750	MS.gene44407:CDS
CTCCTGTGAGCTTGCAGAAG+AGG	0.447057	1.1:-80279728	None:intergenic
ATTGATTCTCTCAGGGAAGC+TGG	0.452667	1.1:+80279907	MS.gene44407:CDS
AAGAGAGAACAGAGGTATCA+TGG	0.461247	1.1:+80279868	MS.gene44407:CDS
GTTGCTGCAGCTGCAAAGAT+TGG	0.469361	1.1:+80279787	MS.gene44407:CDS
AGTTTCCAGGCTTGATCTCA+AGG	0.483054	1.1:-80279755	None:intergenic
TGGTTATCCCACCTCCAGTC+AGG	0.492573	1.1:+80279602	MS.gene44407:CDS
TTCAGATATTGTGTAAGTCT+GGG	0.506970	1.1:-80279628	None:intergenic
TGCCTCTTCTGCAAGCTCAC+AGG	0.512391	1.1:+80279726	MS.gene44407:CDS
GAAGAGGCAACGGTAGCAGT+TGG	0.531232	1.1:-80279712	None:intergenic
GATCAAGCCTGGAAACTACT+CGG	0.540330	1.1:+80279761	MS.gene44407:CDS
AGCGGAACGCCTTTCGCTCA+AGG	0.547714	1.1:+80279819	MS.gene44407:CDS
CAGGAATGTCCTTGAGCGAA+AGG	0.550217	1.1:-80279828	None:intergenic
TTGCTGCAGCTGCAAAGATT+GGG	0.550997	1.1:+80279788	MS.gene44407:CDS
AGCGGTTATTGATTCTCTCA+GGG	0.559363	1.1:+80279900	MS.gene44407:CDS
TTCAAGTGAACAGAAACAGC+AGG	0.564698	1.1:-80279847	None:intergenic
TGGGAGGCCTGACTGGAGGT+GGG	0.577993	1.1:-80279609	None:intergenic
AGAACAGAGGTATCATGGAA+AGG	0.588862	1.1:+80279873	MS.gene44407:CDS
ATCAAGCCTGGAAACTACTC+GGG	0.593151	1.1:+80279762	MS.gene44407:CDS
AGTCTGGGAGGCCTGACTGG+AGG	0.599060	1.1:-80279613	None:intergenic
CAACGGTAGCAGTTGGAGTG+TGG	0.604975	1.1:-80279705	None:intergenic
TGAGCTTGCAGAAGAGGCAA+CGG	0.638179	1.1:-80279722	None:intergenic
AGATATTGTGTAAGTCTGGG+AGG	0.649085	1.1:-80279625	None:intergenic
GTATCATGGAAAGGTAAAAG+CGG	0.664247	1.1:+80279882	MS.gene44407:CDS
AAAGATTGGGAAGCTACTAG+CGG	0.716694	1.1:+80279801	MS.gene44407:CDS
TTCACTTGAAGAGAGAACAG+AGG	0.737493	1.1:+80279860	MS.gene44407:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATGCATCTTAAGAACATA+AGG	-	chr1.1:80279655-80279674	None:intergenic	25.0%
	TTCAGATATTGTGTAAGTCT+GGG	-	chr1.1:80279631-80279650	None:intergenic	30.0%
	TTTCAGATATTGTGTAAGTC+TGG	-	chr1.1:80279632-80279651	None:intergenic	30.0%
	GTATCATGGAAAGGTAAAAG+CGG	+	chr1.1:80279882-80279901	MS.gene44407:CDS	35.0%
	AAAGATTGGGAAGCTACTAG+CGG	+	chr1.1:80279801-80279820	MS.gene44407:CDS	40.0%
	AGATATTGTGTAAGTCTGGG+AGG	-	chr1.1:80279628-80279647	None:intergenic	40.0%
	TTCAAGTGAACAGAAACAGC+AGG	-	chr1.1:80279850-80279869	None:intergenic	40.0%
	TTCACTTGAAGAGAGAACAG+AGG	+	chr1.1:80279860-80279879	MS.gene44407:CDS	40.0%
!!	AAGAGAGAACAGAGGTATCA+TGG	+	chr1.1:80279868-80279887	MS.gene44407:CDS	40.0%
!!	AAGCGGTTATTGATTCTCTC+AGG	+	chr1.1:80279899-80279918	MS.gene44407:CDS	40.0%
!!	AGAACAGAGGTATCATGGAA+AGG	+	chr1.1:80279873-80279892	MS.gene44407:CDS	40.0%
!!	AGCGGTTATTGATTCTCTCA+GGG	+	chr1.1:80279900-80279919	MS.gene44407:CDS	40.0%
	AGTTTCCAGGCTTGATCTCA+AGG	-	chr1.1:80279758-80279777	None:intergenic	45.0%
	ATCAAGCCTGGAAACTACTC+GGG	+	chr1.1:80279762-80279781	MS.gene44407:CDS	45.0%
	GATCAAGCCTGGAAACTACT+CGG	+	chr1.1:80279761-80279780	MS.gene44407:CDS	45.0%
	TTGCTGCAGCTGCAAAGATT+GGG	+	chr1.1:80279788-80279807	MS.gene44407:CDS	45.0%
!	ATTGATTCTCTCAGGGAAGC+TGG	+	chr1.1:80279907-80279926	MS.gene44407:CDS	45.0%
	CAACATCCCGAGTAGTTTCC+AGG	-	chr1.1:80279771-80279790	None:intergenic	50.0%
	CAGGAATGTCCTTGAGCGAA+AGG	-	chr1.1:80279831-80279850	None:intergenic	50.0%
	GAAAGCCTTGAGATCAAGCC+TGG	+	chr1.1:80279750-80279769	MS.gene44407:CDS	50.0%
	GTTGCTGCAGCTGCAAAGAT+TGG	+	chr1.1:80279787-80279806	MS.gene44407:CDS	50.0%
	TGAGCTTGCAGAAGAGGCAA+CGG	-	chr1.1:80279725-80279744	None:intergenic	50.0%
	CAACGGTAGCAGTTGGAGTG+TGG	-	chr1.1:80279708-80279727	None:intergenic	55.0%
	CTCCTGTGAGCTTGCAGAAG+AGG	-	chr1.1:80279731-80279750	None:intergenic	55.0%
	GAAGAGGCAACGGTAGCAGT+TGG	-	chr1.1:80279715-80279734	None:intergenic	55.0%
	TGCCTCTTCTGCAAGCTCAC+AGG	+	chr1.1:80279726-80279745	MS.gene44407:CDS	55.0%
	TGGTTATCCCACCTCCAGTC+AGG	+	chr1.1:80279602-80279621	MS.gene44407:CDS	55.0%
	AGCGGAACGCCTTTCGCTCA+AGG	+	chr1.1:80279819-80279838	MS.gene44407:CDS	60.0%
	GTAAGTCTGGGAGGCCTGAC+TGG	-	chr1.1:80279619-80279638	None:intergenic	60.0%
	AGTCTGGGAGGCCTGACTGG+AGG	-	chr1.1:80279616-80279635	None:intergenic	65.0%
	TGGGAGGCCTGACTGGAGGT+GGG	-	chr1.1:80279612-80279631	None:intergenic	65.0%
	CTGGGAGGCCTGACTGGAGG+TGG	-	chr1.1:80279613-80279632	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	80279601	80279945	80279601	ID=MS.gene44407
chr1.1	mRNA	80279601	80279945	80279601	ID=MS.gene44407.t1;Parent=MS.gene44407
chr1.1	exon	80279601	80279945	80279601	ID=MS.gene44407.t1.exon1;Parent=MS.gene44407.t1
chr1.1	CDS	80279601	80279945	80279601	ID=cds.MS.gene44407.t1;Parent=MS.gene44407.t1

Gene Sequence	>MS.gene44407 ATGGTTATCCCACCTCCAGTCAGGCCTCCCAGACTTACACAATATCTGAAACCTTATGTTCTTAAGATGCATTTCACAAATAAGTATGTGAGTGCTCAAGTGATCCACACTCCAACTGCTACCGTTGCCTCTTCTGCAAGCTCACAGGAGAAAGCCTTGAGATCAAGCCTGGAAACTACTCGGGATGTTGCTGCAGCTGCAAAGATTGGGAAGCTACTAGCGGAACGCCTTTCGCTCAAGGACATTCCTGCTGTTTCTGTTCACTTGAAGAGAGAACAGAGGTATCATGGAAAGGTAAAAGCGGTTATTGATTCTCTCAGGGAAGCTGGTGTTAAGTTGCTTTGA
Protein sequence	>MS.gene44407.t1 MVIPPPVRPPRLTQYLKPYVLKMHFTNKYVSAQVIHTPTATVASSASSQEKALRSSLETTRDVAAAAKIGKLLAERLSLKDIPAVSVHLKREQRYHGKVKAVIDSLREAGVKLL