AlfalfaGEDB | MS.gene46458

M. sativa cultivar XinJiangDaYe / MS.gene46458

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46458.t1	XP_013467705.1	98.8	249	2	1	1	248	14	262	6.50E-98	367.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46458.t1	Q84WI0	39.9	268	113	8	1	248	14	253	1.4e-19	98.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46458.t1	A0A072VIV1	98.8	249	2	1	1	248	14	262	4.7e-98	367.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049351	MS.gene46458	0.803779	2.96E-49	-1.69E-46
MS.gene050432	MS.gene46458	0.805618	1.22E-49	-1.69E-46
MS.gene050593	MS.gene46458	0.830717	2.51E-55	-1.69E-46
MS.gene050772	MS.gene46458	0.803873	2.83E-49	-1.69E-46
MS.gene05105	MS.gene46458	0.804893	1.74E-49	-1.69E-46
MS.gene051255	MS.gene46458	0.817045	4.04E-52	-1.69E-46
MS.gene051387	MS.gene46458	0.833942	3.99E-56	-1.69E-46
MS.gene051858	MS.gene46458	0.806827	6.81E-50	-1.69E-46
MS.gene052271	MS.gene46458	0.812312	4.52E-51	-1.69E-46
MS.gene052484	MS.gene46458	0.809577	1.77E-50	-1.69E-46
MS.gene052485	MS.gene46458	0.811852	5.69E-51	-1.69E-46
MS.gene053207	MS.gene46458	0.813731	2.21E-51	-1.69E-46
MS.gene053209	MS.gene46458	0.803248	3.82E-49	-1.69E-46
MS.gene053322	MS.gene46458	0.808665	2.77E-50	-1.69E-46
MS.gene053813	MS.gene46458	0.843304	1.52E-58	-1.69E-46
MS.gene055206	MS.gene46458	0.803325	3.68E-49	-1.69E-46
MS.gene055277	MS.gene46458	0.818443	1.96E-52	-1.69E-46
MS.gene055636	MS.gene46458	0.80095	1.13E-48	-1.69E-46
MS.gene055647	MS.gene46458	0.855638	5.52E-62	-1.69E-46
MS.gene055722	MS.gene46458	0.819959	8.83E-53	-1.69E-46
MS.gene05592	MS.gene46458	0.832378	9.78E-56	-1.69E-46
MS.gene056191	MS.gene46458	0.816762	4.68E-52	-1.69E-46
MS.gene056374	MS.gene46458	0.801722	7.88E-49	-1.69E-46
MS.gene056410	MS.gene46458	0.829359	5.38E-55	-1.69E-46
MS.gene057331	MS.gene46458	0.844106	9.29E-59	-1.69E-46
MS.gene057416	MS.gene46458	0.822731	2.03E-53	-1.69E-46
MS.gene058660	MS.gene46458	0.815567	8.65E-52	-1.69E-46
MS.gene05907	MS.gene46458	0.814819	1.27E-51	-1.69E-46
MS.gene059536	MS.gene46458	0.806618	7.54E-50	-1.69E-46
MS.gene059676	MS.gene46458	0.824834	6.51E-54	-1.69E-46
MS.gene059688	MS.gene46458	0.81055	1.09E-50	-1.69E-46
MS.gene059692	MS.gene46458	0.804561	2.04E-49	-1.69E-46
MS.gene059695	MS.gene46458	0.80391	2.78E-49	-1.69E-46
MS.gene059711	MS.gene46458	0.816951	4.24E-52	-1.69E-46
MS.gene059902	MS.gene46458	0.803414	3.53E-49	-1.69E-46
MS.gene060193	MS.gene46458	0.81537	9.57E-52	-1.69E-46
MS.gene060403	MS.gene46458	0.807822	4.19E-50	-1.69E-46
MS.gene060804	MS.gene46458	0.82667	2.39E-54	-1.69E-46
MS.gene060818	MS.gene46458	0.826808	2.21E-54	-1.69E-46
MS.gene060837	MS.gene46458	0.846974	1.55E-59	-1.69E-46
MS.gene061200	MS.gene46458	0.820434	6.87E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46458.t1	MTR_1g054450	97.189	249	6	1	1	248	14	262	1.56e-180	496
MS.gene46458.t1	MTR_1g013130	46.067	89	42	2	39	125	64	148	2.26e-13	68.2
MS.gene46458.t1	MTR_8g466760	57.143	56	21	1	55	107	77	132	1.03e-11	63.9
MS.gene46458.t1	MTR_3g012010	57.143	56	21	1	55	107	77	132	1.51e-11	63.5
MS.gene46458.t1	MTR_5g008280	40.426	94	48	3	63	152	36	125	2.69e-11	63.2
MS.gene46458.t1	MTR_3g106230	36.129	155	75	6	50	182	43	195	3.70e-11	61.6
MS.gene46458.t1	MTR_3g011990	57.143	56	21	1	55	107	77	132	3.91e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46458.t1	AT3G58070	38.931	262	124	9	1	248	14	253	5.06e-38	134
MS.gene46458.t1	AT2G41940	39.493	276	105	10	1	248	16	257	1.32e-36	130
MS.gene46458.t1	AT5G06650	35.714	252	91	12	1	248	7	191	1.75e-25	99.4
MS.gene46458.t1	AT1G68360	63.265	49	18	0	73	121	71	119	1.45e-14	71.2
MS.gene46458.t1	AT1G67030	44.776	67	32	1	51	112	12	78	4.01e-13	66.6
MS.gene46458.t1	AT1G24625	37.500	112	54	3	31	142	16	111	1.07e-11	62.8
MS.gene46458.t1	AT5G10970	35.849	106	62	3	18	123	50	149	4.99e-11	61.6

Find 52 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGAGGAAAGATCAAATTTA+TGG	0.180908	1.4:+44738923	MS.gene46458:CDS
TTGTTGTTGTTGGTTGTTTC+CGG	0.244675	1.4:-44738424	None:intergenic
TCTCAACAACAACCTATTAA+TGG	0.266540	1.4:+44738811	MS.gene46458:CDS
TGTTGGTTGTTTCCGGAGAC+TGG	0.304545	1.4:-44738417	None:intergenic
GCTAAAGGACTACCATTAAT+AGG	0.319842	1.4:-44738823	None:intergenic
CGCCTCTTCGGTATCGAATT+CGG	0.358178	1.4:+44738367	MS.gene46458:CDS
TACTCTGAACTGTGCCGCTT+GGG	0.363029	1.4:-44738858	None:intergenic
ATGCCTTTCTCTTTGTTAGT+AGG	0.371500	1.4:-44738343	None:intergenic
AGTAGGAGGAGGACGGATGA+AGG	0.388482	1.4:-44738326	None:intergenic
ACGTAACGACCATGTTAGTT+TGG	0.409802	1.4:+44739008	MS.gene46458:CDS
TGAGAGTAAGAAGAAGTTGT+TGG	0.413123	1.4:-44738793	None:intergenic
TTTACCTTTGCTTGCTTCTG+AGG	0.423534	1.4:+44738906	MS.gene46458:CDS
ACAACACCTTCAATCAACTA+TGG	0.424652	1.4:+44738615	MS.gene46458:CDS
TTCTCTTTGTTAGTAGGAGG+AGG	0.445901	1.4:-44738337	None:intergenic
TTTCCTACTTCACAAGCCTT+AGG	0.446992	1.4:+44738547	MS.gene46458:CDS
GTGTTCCATGTTGGATAAGA+AGG	0.460404	1.4:-44738745	None:intergenic
GAGAAAGGCATCCGCCTCTT+CGG	0.461897	1.4:+44738355	MS.gene46458:CDS
GGACGTTCATGGTTGAAACT+AGG	0.467117	1.4:-44738883	None:intergenic
CCACCTAAGGCTTGTGAAGT+AGG	0.467927	1.4:-44738550	None:intergenic
CATGTACCATAGTTGATTGA+AGG	0.471062	1.4:-44738621	None:intergenic
CCTTTCTCTTTGTTAGTAGG+AGG	0.473358	1.4:-44738340	None:intergenic
GACGTTCATGGTTGAAACTA+GGG	0.477100	1.4:-44738882	None:intergenic
AGAGAGGATGCTGTTGTTGA+TGG	0.483034	1.4:-44738681	None:intergenic
GCAAAGGTAAAGGACGTTCA+TGG	0.484971	1.4:-44738894	None:intergenic
AGACTGGGAGGACTCGTCTT+CGG	0.490966	1.4:-44738401	None:intergenic
TTACTCTGAACTGTGCCGCT+TGG	0.499118	1.4:-44738859	None:intergenic
GTAGGAGGAGGACGGATGAA+GGG	0.505234	1.4:-44738325	None:intergenic
AGACGAGTCCTCCCAGTCTC+CGG	0.509456	1.4:+44738405	MS.gene46458:CDS
GCTGTTGTTGATGGTGATGA+TGG	0.514460	1.4:-44738672	None:intergenic
CATGGAACACAAACTCAACT+AGG	0.515152	1.4:+44738758	MS.gene46458:CDS
TTCGGTATCGAATTCGGTGG+TGG	0.520561	1.4:+44738373	MS.gene46458:CDS
GTTGGTTGTTTCCGGAGACT+GGG	0.526993	1.4:-44738416	None:intergenic
GTTGAGTTTGTGTTCCATGT+TGG	0.535384	1.4:-44738754	None:intergenic
CAACAACATTAACTCGTTCG+AGG	0.551035	1.4:+44738444	MS.gene46458:CDS
CCTCCTACTAACAAAGAGAA+AGG	0.564200	1.4:+44738340	MS.gene46458:CDS
AATTTATGGCACAGTGGACA+AGG	0.565618	1.4:+44738937	MS.gene46458:CDS
CCTACTTCACAAGCCTTAGG+TGG	0.566151	1.4:+44738550	MS.gene46458:CDS
CTTTGTTAGTAGGAGGAGGA+CGG	0.568267	1.4:-44738333	None:intergenic
AGTAAGAAGAAGTTGTTGGA+TGG	0.569777	1.4:-44738789	None:intergenic
TCAGAAGCAAGCAAAGGTAA+AGG	0.571923	1.4:-44738904	None:intergenic
CATCACCTTCTTATCCAACA+TGG	0.575046	1.4:+44738740	MS.gene46458:CDS
TGGTGATGATGGTAGAGATG+TGG	0.592964	1.4:-44738661	None:intergenic
GGTGATGATGATAATGATGA+TGG	0.595521	1.4:-44738724	None:intergenic
TTATGCGAAGCAGAAGAAGA+AGG	0.620504	1.4:-44738964	None:intergenic
CGAATTCGATACCGAAGAGG+CGG	0.654284	1.4:-44738366	None:intergenic
CTTTCCTCAGAAGCAAGCAA+AGG	0.654611	1.4:-44738910	None:intergenic
GGTTGTTTCCGGAGACTGGG+AGG	0.658133	1.4:-44738413	None:intergenic
CACCGAATTCGATACCGAAG+AGG	0.659063	1.4:-44738369	None:intergenic
CTCTTCGGTATCGAATTCGG+TGG	0.666246	1.4:+44738370	MS.gene46458:CDS
GATGATGTGAATCTATAGAG+AGG	0.674735	1.4:-44738697	None:intergenic
GATGAAGATCCAAACTAACA+TGG	0.681809	1.4:-44739017	None:intergenic
AGATCAAATTTATGGCACAG+TGG	0.730014	1.4:+44738931	MS.gene46458:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTGAGGAAAGATCAAATTTA+TGG	+	chr1.4:44738923-44738942	MS.gene46458:CDS	30.0%
	TCTCAACAACAACCTATTAA+TGG	+	chr1.4:44738811-44738830	MS.gene46458:CDS	30.0%
!	GTTAATGTTGTTGTTGTTGT+TGG	-	chr1.4:44738437-44738456	None:intergenic	30.0%
	ACAACACCTTCAATCAACTA+TGG	+	chr1.4:44738615-44738634	MS.gene46458:CDS	35.0%
	AGATCAAATTTATGGCACAG+TGG	+	chr1.4:44738931-44738950	MS.gene46458:CDS	35.0%
	AGTAAGAAGAAGTTGTTGGA+TGG	-	chr1.4:44738792-44738811	None:intergenic	35.0%
	ATGCCTTTCTCTTTGTTAGT+AGG	-	chr1.4:44738346-44738365	None:intergenic	35.0%
	CATGTACCATAGTTGATTGA+AGG	-	chr1.4:44738624-44738643	None:intergenic	35.0%
	GATGAAGATCCAAACTAACA+TGG	-	chr1.4:44739020-44739039	None:intergenic	35.0%
	GATGATGTGAATCTATAGAG+AGG	-	chr1.4:44738700-44738719	None:intergenic	35.0%
	GCTAAAGGACTACCATTAAT+AGG	-	chr1.4:44738826-44738845	None:intergenic	35.0%
	TGAGAGTAAGAAGAAGTTGT+TGG	-	chr1.4:44738796-44738815	None:intergenic	35.0%
!	GGTGATGATGATAATGATGA+TGG	-	chr1.4:44738727-44738746	None:intergenic	35.0%
!	TTGTTGTTGTTGGTTGTTTC+CGG	-	chr1.4:44738427-44738446	None:intergenic	35.0%
!!!	ATGGTAGTCCTTTAGCTTTT+TGG	+	chr1.4:44738830-44738849	MS.gene46458:CDS	35.0%
	AATTTATGGCACAGTGGACA+AGG	+	chr1.4:44738937-44738956	MS.gene46458:CDS	40.0%
	ACGTAACGACCATGTTAGTT+TGG	+	chr1.4:44739008-44739027	MS.gene46458:CDS	40.0%
	CAACAACATTAACTCGTTCG+AGG	+	chr1.4:44738444-44738463	MS.gene46458:CDS	40.0%
	CATCACCTTCTTATCCAACA+TGG	+	chr1.4:44738740-44738759	MS.gene46458:CDS	40.0%
	CATGGAACACAAACTCAACT+AGG	+	chr1.4:44738758-44738777	MS.gene46458:CDS	40.0%
	CCTCCTACTAACAAAGAGAA+AGG	+	chr1.4:44738340-44738359	MS.gene46458:CDS	40.0%
	CCTTTCTCTTTGTTAGTAGG+AGG	-	chr1.4:44738343-44738362	None:intergenic	40.0%
	GACGTTCATGGTTGAAACTA+GGG	-	chr1.4:44738885-44738904	None:intergenic	40.0%
	GTGTTCCATGTTGGATAAGA+AGG	-	chr1.4:44738748-44738767	None:intergenic	40.0%
	GTTGAGTTTGTGTTCCATGT+TGG	-	chr1.4:44738757-44738776	None:intergenic	40.0%
	TCAGAAGCAAGCAAAGGTAA+AGG	-	chr1.4:44738907-44738926	None:intergenic	40.0%
	TTATGCGAAGCAGAAGAAGA+AGG	-	chr1.4:44738967-44738986	None:intergenic	40.0%
	TTCTCTTTGTTAGTAGGAGG+AGG	-	chr1.4:44738340-44738359	None:intergenic	40.0%
	TTTACCTTTGCTTGCTTCTG+AGG	+	chr1.4:44738906-44738925	MS.gene46458:CDS	40.0%
	TTTCCTACTTCACAAGCCTT+AGG	+	chr1.4:44738547-44738566	MS.gene46458:CDS	40.0%
	AGAGAGGATGCTGTTGTTGA+TGG	-	chr1.4:44738684-44738703	None:intergenic	45.0%
	CTTTCCTCAGAAGCAAGCAA+AGG	-	chr1.4:44738913-44738932	None:intergenic	45.0%
	CTTTGTTAGTAGGAGGAGGA+CGG	-	chr1.4:44738336-44738355	None:intergenic	45.0%
	GCAAAGGTAAAGGACGTTCA+TGG	-	chr1.4:44738897-44738916	None:intergenic	45.0%
	GGACGTTCATGGTTGAAACT+AGG	-	chr1.4:44738886-44738905	None:intergenic	45.0%
!!	GCTGTTGTTGATGGTGATGA+TGG	-	chr1.4:44738675-44738694	None:intergenic	45.0%
!!	TGCGTTTTGATGACCACCTA+AGG	-	chr1.4:44738566-44738585	None:intergenic	45.0%
!!	TGGTGATGATGGTAGAGATG+TGG	-	chr1.4:44738664-44738683	None:intergenic	45.0%
	CACCGAATTCGATACCGAAG+AGG	-	chr1.4:44738372-44738391	None:intergenic	50.0%
	CCACCTAAGGCTTGTGAAGT+AGG	-	chr1.4:44738553-44738572	None:intergenic	50.0%
	CCTACTTCACAAGCCTTAGG+TGG	+	chr1.4:44738550-44738569	MS.gene46458:CDS	50.0%
	CGAATTCGATACCGAAGAGG+CGG	-	chr1.4:44738369-44738388	None:intergenic	50.0%
	GGGACACGCCAAAAAGCTAA+AGG	-	chr1.4:44738841-44738860	None:intergenic	50.0%
!	CGCCTCTTCGGTATCGAATT+CGG	+	chr1.4:44738367-44738386	MS.gene46458:CDS	50.0%
!	CTCTTCGGTATCGAATTCGG+TGG	+	chr1.4:44738370-44738389	MS.gene46458:CDS	50.0%
!	GTTGGTTGTTTCCGGAGACT+GGG	-	chr1.4:44738419-44738438	None:intergenic	50.0%
!	TGTTGGTTGTTTCCGGAGAC+TGG	-	chr1.4:44738420-44738439	None:intergenic	50.0%
!	TTACTCTGAACTGTGCCGCT+TGG	-	chr1.4:44738862-44738881	None:intergenic	50.0%
!	TTCGGTATCGAATTCGGTGG+TGG	+	chr1.4:44738373-44738392	MS.gene46458:CDS	50.0%
!!	TACTCTGAACTGTGCCGCTT+GGG	-	chr1.4:44738861-44738880	None:intergenic	50.0%
	AGACTGGGAGGACTCGTCTT+CGG	-	chr1.4:44738404-44738423	None:intergenic	55.0%
	AGTAGGAGGAGGACGGATGA+AGG	-	chr1.4:44738329-44738348	None:intergenic	55.0%
	GTAGGAGGAGGACGGATGAA+GGG	-	chr1.4:44738328-44738347	None:intergenic	55.0%
!!	GAGAAAGGCATCCGCCTCTT+CGG	+	chr1.4:44738355-44738374	MS.gene46458:CDS	55.0%
!!	GCTTTTTGGCGTGTCCCAAG+CGG	+	chr1.4:44738844-44738863	MS.gene46458:CDS	55.0%
	AGACGAGTCCTCCCAGTCTC+CGG	+	chr1.4:44738405-44738424	MS.gene46458:CDS	60.0%
!	GGTTGTTTCCGGAGACTGGG+AGG	-	chr1.4:44738416-44738435	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	44738298	44739044	44738298	ID=MS.gene46458
chr1.4	mRNA	44738298	44739044	44738298	ID=MS.gene46458.t1;Parent=MS.gene46458
chr1.4	exon	44738298	44739044	44738298	ID=MS.gene46458.t1.exon1;Parent=MS.gene46458.t1
chr1.4	CDS	44738298	44739044	44738298	ID=cds.MS.gene46458.t1;Parent=MS.gene46458.t1

Gene Sequence

>MS.gene46458

ATGAACGTTGAGTCATTCTCTCAACTTCCCTTCATCCGTCCTCCTCCTACTAACAAAGAGAAAGGCATCCGCCTCTTCGGTATCGAATTCGGTGGTGGCGCAACCGAAGACGAGTCCTCCCAGTCTCCGGAAACAACCAACAACAACAACAACATTAACTCGTTCGAGGTTGACAACAACAACAACAAAGACAACAACAATAACAACGAAAACAACAGAAGATTTGAATGTCATTACTGTTGTAGAAACTTTCCTACTTCACAAGCCTTAGGTGGTCATCAAAACGCACACAAAAGAGAACGTCAACACGCAAAACGACAACACCTTCAATCAACTATGGTACATGCTACTACTTTCTCTGATCCACATCTCTACCATCATCACCATCAACAACAGCATCCTCTCTATAGATTCACATCATCATCACCATCATCATTATCATCATCACCTTCTTATCCAACATGGAACACAAACTCAACTAGGTTCTATAACCATCCAACAACTTCTTCTTACTCTCAACAACAACCTATTAATGGTAGTCCTTTAGCTTTTTGGCGTGTCCCAAGCGGCACAGTTCAGAGTAACCCTAGTTTCAACCATGAACGTCCTTTACCTTTGCTTGCTTCTGAGGAAAGATCAAATTTATGGCACAGTGGACAAGGAGTACCTTCTTCTTCTGCTTCGCATAATCGTTACGTTTATGACTCTAAACGTAACGACCATGTTAGTTTGGATCTTCATCTCTAA

Protein sequence

>MS.gene46458.t1

MNVESFSQLPFIRPPPTNKEKGIRLFGIEFGGGATEDESSQSPETTNNNNNINSFEVDNNNNKDNNNNNENNRRFECHYCCRNFPTSQALGGHQNAHKRERQHAKRQHLQSTMVHATTFSDPHLYHHHHQQQHPLYRFTSSSPSSLSSSPSYPTWNTNSTRFYNHPTTSSYSQQQPINGSPLAFWRVPSGTVQSNPSFNHERPLPLLASEERSNLWHSGQGVPSSSASHNRYVYDSKRNDHVSLDLHL