AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55657


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55657.t1 MTR_3g064020 85.020 247 34 1 1 244 1 247 1.17e-155 432
MS.gene55657.t1 MTR_5g076250 62.857 245 78 5 1 239 1 238 1.38e-95 280
MS.gene55657.t1 MTR_3g063990 54.980 251 71 4 9 221 3 249 8.71e-84 251
MS.gene55657.t1 MTR_1g057650 51.653 242 103 6 1 236 1 234 9.58e-74 225
MS.gene55657.t1 MTR_7g117750 43.983 241 121 5 1 236 1 232 1.63e-60 191
MS.gene55657.t1 MTR_3g063980 57.534 73 27 1 167 239 56 124 7.45e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55657.t1 AT3G02700 57.917 240 89 6 1 236 1 232 2.39e-81 244
MS.gene55657.t1 AT5G06370 50.826 242 105 6 1 236 1 234 8.39e-74 225
MS.gene55657.t1 AT5G16360 47.101 276 96 8 1 236 1 266 4.39e-70 216
MS.gene55657.t1 AT4G00905 45.714 245 120 5 1 236 1 241 4.99e-63 198
MS.gene55657.t1 AT5G16330 47.325 243 98 8 1 236 9 228 6.93e-62 194
MS.gene55657.t1 AT1G01225 47.391 230 112 3 1 226 1 225 1.58e-61 194

Find 56 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTAGATTGCTTTCTTTC+AGG 0.124117 3.3:-57652507 MS.gene55657:CDS
GCTTTATATCTTCTTGAAAA+TGG 0.152944 3.3:-57652369 MS.gene55657:CDS
AAAAGATTGGTTTCTGATAT+TGG 0.189791 3.3:-57652141 MS.gene55657:CDS
ATGTTTCCGATCAGTCTTAT+TGG 0.247318 3.3:+57652917 None:intergenic
CACAGTTATTCTTGAAGATA+TGG 0.269187 3.3:+57652334 None:intergenic
TATCTTCTTGAAAATGGATT+TGG 0.299009 3.3:-57652363 MS.gene55657:CDS
TTTGCAGTTTATTGCAAAAC+AGG 0.337490 3.3:-57652306 MS.gene55657:CDS
TGCATGTGCCTCCTCTAGCT+TGG 0.338272 3.3:+57652433 None:intergenic
CCGCACCGTCACGACATCTT+TGG 0.342473 3.3:+57652559 None:intergenic
GAACTCTACCTCTTTCAATA+TGG 0.373949 3.3:-57652480 MS.gene55657:CDS
AACTTACTGCATTAAAAGAT+TGG 0.377765 3.3:-57652154 MS.gene55657:CDS
ATGGTGATCCATTTCACAAT+TGG 0.385236 3.3:-57652660 MS.gene55657:CDS
CTGGGGATCATATTTACTCT+TGG 0.413378 3.3:-57652887 MS.gene55657:CDS
GCATGTGCCTCCTCTAGCTT+GGG 0.419205 3.3:+57652434 None:intergenic
CACATGCACCCTAGCTGCTT+CGG 0.423293 3.3:-57652418 MS.gene55657:CDS
ATCCATTTCACAATTGGATC+AGG 0.464773 3.3:-57652654 MS.gene55657:CDS
ATCGGAAACATCTAAAACCT+GGG 0.479101 3.3:-57652905 MS.gene55657:CDS
GGGATCATATTTACTCTTGG+AGG 0.483005 3.3:-57652884 MS.gene55657:CDS
ACAGGAATATATGTTGAAGA+TGG 0.483154 3.3:-57652684 MS.gene55657:intron
GTCCTGATCCAATTGTGAAA+TGG 0.487607 3.3:+57652652 None:intergenic
GGATCAGGACAGCAAGCTGC+TGG 0.487944 3.3:-57652639 MS.gene55657:CDS
CGAAAGAACTTGTAAAGAAA+CGG 0.498819 3.3:+57652598 None:intergenic
ATACCTGAGTTGACCATGGA+TGG 0.501421 3.3:-57652069 MS.gene55657:CDS
TCATATTTACTCTTGGAGGC+AGG 0.503930 3.3:-57652880 MS.gene55657:CDS
GCTGGCAGCAGCTGAATAAG+AGG 0.511661 3.3:+57652245 None:intergenic
TGAAAGAAAGCAATCTAAGC+AGG 0.512899 3.3:+57652509 None:intergenic
ACATCTTTGGCATGGAATGT+TGG 0.518788 3.3:+57652572 None:intergenic
TTCAAGAAGATATAAAGCAC+GGG 0.519285 3.3:+57652374 None:intergenic
TGGAGACACCATATTGAAAG+AGG 0.529495 3.3:+57652472 None:intergenic
CAGTTGGATCCGAAGCAGCT+AGG 0.530992 3.3:+57652409 None:intergenic
AGTTGGATCCGAAGCAGCTA+GGG 0.533238 3.3:+57652410 None:intergenic
GTCGTGACGGTGCGGAAACA+AGG 0.535193 3.3:-57652551 MS.gene55657:CDS
AGTCTACTAGCTTCCATCCA+TGG 0.540139 3.3:+57652056 None:intergenic
GATCGGAAACATCTAAAACC+TGG 0.543021 3.3:-57652906 MS.gene55657:CDS
AAGAAACGGTATAAAATCGC+TGG 0.543391 3.3:+57652612 None:intergenic
TTTCAAGAAGATATAAAGCA+CGG 0.545122 3.3:+57652373 None:intergenic
CGGGGAAGGACCTCTTCAGT+TGG 0.547650 3.3:+57652393 None:intergenic
GGTAGGGCTTTGGTTTGCTG+TGG 0.552956 3.3:-57652177 MS.gene55657:CDS
TCAACCTCAAAAGGCTGCAG+TGG 0.553525 3.3:-57652276 MS.gene55657:CDS
TTAGATTGCTTTCTTTCAGG+AGG 0.554330 3.3:-57652504 MS.gene55657:CDS
TGCTTTCCAATAAGACTGAT+CGG 0.558642 3.3:-57652923 MS.gene55657:CDS
CTTCCATCCATGGTCAACTC+AGG 0.559095 3.3:+57652066 None:intergenic
TGATGAAGAGTCTACAGTGC+TGG 0.563974 3.3:+57652227 None:intergenic
CAAGAGTAAATATGATCCCC+AGG 0.568302 3.3:+57652888 None:intergenic
CTGTAGACTCTTCATCACTT+CGG 0.584097 3.3:-57652221 MS.gene55657:CDS
CCGTCACGACATCTTTGGCA+TGG 0.598030 3.3:+57652564 None:intergenic
GAAGATATAAAGCACGGGGA+AGG 0.600493 3.3:+57652379 None:intergenic
AATATATGTTGAAGATGGAA+TGG 0.601381 3.3:-57652679 MS.gene55657:CDS
GCTTACCTCTATGCACATCA+TGG 0.604488 3.3:-57652858 MS.gene55657:intron
GGTGCGGAAACAAGGACTCA+TGG 0.614117 3.3:-57652543 MS.gene55657:CDS
GATGACCATGATGTGCATAG+AGG 0.641641 3.3:+57652853 None:intergenic
TGCTTCGGATCCAACTGAAG+AGG 0.647462 3.3:-57652403 MS.gene55657:CDS
CCAAAGATGTCGTGACGGTG+CGG 0.654870 3.3:-57652559 MS.gene55657:CDS
CCATGCCAAAGATGTCGTGA+CGG 0.658966 3.3:-57652564 MS.gene55657:CDS
TCAAGAAGATATAAAGCACG+GGG 0.677622 3.3:+57652375 None:intergenic
TCGGAAACATCTAAAACCTG+GGG 0.712111 3.3:-57652904 MS.gene55657:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTTGATACATAAGAAAA+TGG + chr3.3:57652217-57652236 None:intergenic 20.0%
! AACTTACTGCATTAAAAGAT+TGG - chr3.3:57652838-57652857 MS.gene55657:intron 25.0%
! AATATATGTTGAAGATGGAA+TGG - chr3.3:57652313-57652332 MS.gene55657:CDS 25.0%
! GCTTTATATCTTCTTGAAAA+TGG - chr3.3:57652623-57652642 MS.gene55657:CDS 25.0%
! TTTCAAGAAGATATAAAGCA+CGG + chr3.3:57652622-57652641 None:intergenic 25.0%
!! TATCTTCTTGAAAATGGATT+TGG - chr3.3:57652629-57652648 MS.gene55657:CDS 25.0%
!! TTGTGCAAGAAAGTATAAAT+TGG + chr3.3:57652187-57652206 None:intergenic 25.0%
!!! AAAAGATTGGTTTCTGATAT+TGG - chr3.3:57652851-57652870 MS.gene55657:intron 25.0%
ACAGGAATATATGTTGAAGA+TGG - chr3.3:57652308-57652327 MS.gene55657:CDS 30.0%
CACAGTTATTCTTGAAGATA+TGG + chr3.3:57652661-57652680 None:intergenic 30.0%
CGAAAGAACTTGTAAAGAAA+CGG + chr3.3:57652397-57652416 None:intergenic 30.0%
TCAGTATGATAGTTATGCAT+AGG + chr3.3:57652242-57652261 None:intergenic 30.0%
TTCAAGAAGATATAAAGCAC+GGG + chr3.3:57652621-57652640 None:intergenic 30.0%
TTGGGCTAAAAAATGTAGTT+TGG + chr3.3:57652543-57652562 None:intergenic 30.0%
TTTGCAGTTTATTGCAAAAC+AGG - chr3.3:57652686-57652705 MS.gene55657:intron 30.0%
!!! AACTCAGGTATTTTTTCAAC+TGG + chr3.3:57652914-57652933 None:intergenic 30.0%
AAGAAACGGTATAAAATCGC+TGG + chr3.3:57652383-57652402 None:intergenic 35.0%
ATCCATTTCACAATTGGATC+AGG - chr3.3:57652338-57652357 MS.gene55657:CDS 35.0%
ATGGTGATCCATTTCACAAT+TGG - chr3.3:57652332-57652351 MS.gene55657:CDS 35.0%
CGGCATTTCTGTAATATAAC+AGG - chr3.3:57652290-57652309 MS.gene55657:CDS 35.0%
GAACTCTACCTCTTTCAATA+TGG - chr3.3:57652512-57652531 MS.gene55657:CDS 35.0%
TCAAGAAGATATAAAGCACG+GGG + chr3.3:57652620-57652639 None:intergenic 35.0%
TCATATTTGTGTCCTGCTAA+CGG - chr3.3:57652270-57652289 MS.gene55657:CDS 35.0%
TGAAAGAAAGCAATCTAAGC+AGG + chr3.3:57652486-57652505 None:intergenic 35.0%
TGCTTAGATTGCTTTCTTTC+AGG - chr3.3:57652485-57652504 MS.gene55657:CDS 35.0%
TGCTTTCCAATAAGACTGAT+CGG - chr3.3:57652069-57652088 MS.gene55657:CDS 35.0%
TTAGATTGCTTTCTTTCAGG+AGG - chr3.3:57652488-57652507 MS.gene55657:CDS 35.0%
! AAAAATACCTGAGTTGACCA+TGG - chr3.3:57652919-57652938 MS.gene55657:CDS 35.0%
! ATCGGAAACATCTAAAACCT+GGG - chr3.3:57652087-57652106 MS.gene55657:CDS 35.0%
! ATGTTTCCGATCAGTCTTAT+TGG + chr3.3:57652078-57652097 None:intergenic 35.0%
! TTTGTGAACTCCAGTCTTTT+TGG - chr3.3:57652794-57652813 MS.gene55657:intron 35.0%
!!! GGTCTTTTTTCAACCTCAAA+AGG - chr3.3:57652707-57652726 MS.gene55657:intron 35.0%
ACATCTTTGGCATGGAATGT+TGG + chr3.3:57652423-57652442 None:intergenic 40.0%
ATTACAGAAATGCCGTTAGC+AGG + chr3.3:57652285-57652304 None:intergenic 40.0%
CAAGAGTAAATATGATCCCC+AGG + chr3.3:57652107-57652126 None:intergenic 40.0%
CTGGGGATCATATTTACTCT+TGG - chr3.3:57652105-57652124 MS.gene55657:CDS 40.0%
CTGTAGACTCTTCATCACTT+CGG - chr3.3:57652771-57652790 MS.gene55657:intron 40.0%
GGGATCATATTTACTCTTGG+AGG - chr3.3:57652108-57652127 MS.gene55657:CDS 40.0%
GTCCTGATCCAATTGTGAAA+TGG + chr3.3:57652343-57652362 None:intergenic 40.0%
TCATATTTACTCTTGGAGGC+AGG - chr3.3:57652112-57652131 MS.gene55657:CDS 40.0%
TGGAGACACCATATTGAAAG+AGG + chr3.3:57652523-57652542 None:intergenic 40.0%
! GATCGGAAACATCTAAAACC+TGG - chr3.3:57652086-57652105 MS.gene55657:CDS 40.0%
! TCGGAAACATCTAAAACCTG+GGG - chr3.3:57652088-57652107 MS.gene55657:CDS 40.0%
!! GAACTCCAGTCTTTTTGGTA+GGG - chr3.3:57652799-57652818 MS.gene55657:intron 40.0%
!! TGAACTCCAGTCTTTTTGGT+AGG - chr3.3:57652798-57652817 MS.gene55657:intron 40.0%
!!! CATTTTTTAGCCCAAGCTAG+AGG - chr3.3:57652548-57652567 MS.gene55657:CDS 40.0%
CAAAGCCCTACCAAAAAGAC+TGG + chr3.3:57652807-57652826 None:intergenic 45.0%
GAAGATATAAAGCACGGGGA+AGG + chr3.3:57652616-57652635 None:intergenic 45.0%
GATGACCATGATGTGCATAG+AGG + chr3.3:57652142-57652161 None:intergenic 45.0%
GCTTACCTCTATGCACATCA+TGG - chr3.3:57652134-57652153 MS.gene55657:CDS 45.0%
TGATGAAGAGTCTACAGTGC+TGG + chr3.3:57652768-57652787 None:intergenic 45.0%
! ATACCTGAGTTGACCATGGA+TGG - chr3.3:57652923-57652942 MS.gene55657:CDS 45.0%
!! CAGTCTTTTTGGTAGGGCTT+TGG - chr3.3:57652805-57652824 MS.gene55657:intron 45.0%
!! TTTTTAGCCCAAGCTAGAGG+AGG - chr3.3:57652551-57652570 MS.gene55657:CDS 45.0%
AGTTGGATCCGAAGCAGCTA+GGG + chr3.3:57652585-57652604 None:intergenic 50.0%
CCATGCCAAAGATGTCGTGA+CGG - chr3.3:57652428-57652447 MS.gene55657:CDS 50.0%
CTTCCATCCATGGTCAACTC+AGG + chr3.3:57652929-57652948 None:intergenic 50.0%
TCAACCTCAAAAGGCTGCAG+TGG - chr3.3:57652716-57652735 MS.gene55657:intron 50.0%
TGCTTCGGATCCAACTGAAG+AGG - chr3.3:57652589-57652608 MS.gene55657:CDS 50.0%
! TTGACCACTGCAGCCTTTTG+AGG + chr3.3:57652723-57652742 None:intergenic 50.0%
CACATGCACCCTAGCTGCTT+CGG - chr3.3:57652574-57652593 MS.gene55657:CDS 55.0%
CAGTTGGATCCGAAGCAGCT+AGG + chr3.3:57652586-57652605 None:intergenic 55.0%
CCAAAGATGTCGTGACGGTG+CGG - chr3.3:57652433-57652452 MS.gene55657:CDS 55.0%
CCGTCACGACATCTTTGGCA+TGG + chr3.3:57652431-57652450 None:intergenic 55.0%
GCTGGCAGCAGCTGAATAAG+AGG + chr3.3:57652750-57652769 None:intergenic 55.0%
GGTGCGGAAACAAGGACTCA+TGG - chr3.3:57652449-57652468 MS.gene55657:CDS 55.0%
TGCATGTGCCTCCTCTAGCT+TGG + chr3.3:57652562-57652581 None:intergenic 55.0%
! GCATGTGCCTCCTCTAGCTT+GGG + chr3.3:57652561-57652580 None:intergenic 55.0%
!! GGTAGGGCTTTGGTTTGCTG+TGG - chr3.3:57652815-57652834 MS.gene55657:intron 55.0%
CCGCACCGTCACGACATCTT+TGG + chr3.3:57652436-57652455 None:intergenic 60.0%
CGGGGAAGGACCTCTTCAGT+TGG + chr3.3:57652602-57652621 None:intergenic 60.0%
GGATCAGGACAGCAAGCTGC+TGG - chr3.3:57652353-57652372 MS.gene55657:CDS 60.0%
GTCGTGACGGTGCGGAAACA+AGG - chr3.3:57652441-57652460 MS.gene55657:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 57652062 57652952 57652062 ID=MS.gene55657
chr3.3 mRNA 57652062 57652952 57652062 ID=MS.gene55657.t1;Parent=MS.gene55657
chr3.3 exon 57652859 57652952 57652859 ID=MS.gene55657.t1.exon1;Parent=MS.gene55657.t1
chr3.3 CDS 57652859 57652952 57652859 ID=cds.MS.gene55657.t1;Parent=MS.gene55657.t1
chr3.3 exon 57652062 57652702 57652062 ID=MS.gene55657.t1.exon2;Parent=MS.gene55657.t1
chr3.3 CDS 57652062 57652702 57652062 ID=cds.MS.gene55657.t1;Parent=MS.gene55657.t1
Gene Sequence

>MS.gene55657

ATGGGAGTGCTTTCCAATAAGACTGATCGGAAACATCTAAAACCTGGGGATCATATTTACTCTTGGAGGCAGGCTTACCTCTATGCACATCATGGTCATCTCTTTTCTCTTATATTTATCTGCCAATTTATACTTTCTTGCACAACTATGAACCATTTTCTTATGTATCAAACTTATCCTATGCATAACTATCATACTGATGTTAATGTCATATTTGTGTCCTGCTAACGGCATTTCTGTAATATAACAGGAATATATGTTGAAGATGGAATGGTGATCCATTTCACAATTGGATCAGGACAGCAAGCTGCTGGAACACCAGCGATTTTATACCGTTTCTTTACAAGTTCTTTCGATGCCAACATTCCATGCCAAAGATGTCGTGACGGTGCGGAAACAAGGACTCATGGTGTCTTCTCATCCTGCTTAGATTGCTTTCTTTCAGGAGGTGAACTCTACCTCTTTCAATATGGTGTCTCCAAACTACATTTTTTAGCCCAAGCTAGAGGAGGCACATGCACCCTAGCTGCTTCGGATCCAACTGAAGAGGTCCTTCCCCGTGCTTTATATCTTCTTGAAAATGGATTTGGTGACTACCATATCTTCAAGAATAACTGTGAAGATTTTGCAGTTTATTGCAAAACAGGTCTTTTTTCAACCTCAAAAGGCTGCAGTGGTCAAGCAGCCTCTTATTCAGCTGCTGCCAGCACTGTAGACTCTTCATCACTTCGGTTTGTGAACTCCAGTCTTTTTGGTAGGGCTTTGGTTTGCTGTGGAACTTACTGCATTAAAAGATTGGTTTCTGATATTGGATTTCGTTATGAAGTAATTAAAGATGTAACTAAAGTTCCAGTTGAAAAAATACCTGAGTTGACCATGGATGGAAGCTAG

Protein sequence

>MS.gene55657.t1

MGVLSNKTDRKHLKPGDHIYSWRQAYLYAHHGIYVEDGMVIHFTIGSGQQAAGTPAILYRFFTSSFDANIPCQRCRDGAETRTHGVFSSCLDCFLSGGELYLFQYGVSKLHFLAQARGGTCTLAASDPTEEVLPRALYLLENGFGDYHIFKNNCEDFAVYCKTGLFSTSKGCSGQAASYSAAASTVDSSSLRFVNSSLFGRALVCCGTYCIKRLVSDIGFRYEVIKDVTKVPVEKIPELTMDGS