AlfalfaGEDB | MS.gene55687

M. sativa cultivar XinJiangDaYe / MS.gene55687

KEGG: K01520
GO: GO:0000287 GO:0003674 GO:0003824 GO:0004170 GO:0005488 GO:0006139 GO:0006220 GO:0006221 GO:0006226 GO:0006244 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009141 GO:0009143 GO:0009147 GO:0009149 GO:0009157 GO:0009162 GO:0009165 GO:0009166 GO:0009176 GO:0009177 GO:0009200 GO:0009204 GO:0009211 GO:0009213 GO:0009219 GO:0009221 GO:0009223 GO:0009262 GO:0009263 GO:0009264 GO:0009265 GO:0009394 GO:0009987 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019692 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0043167 GO:0043169 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0046078 GO:0046080 GO:0046081 GO:0046385 GO:0046386 GO:0046434 GO:0046483 GO:0046700 GO:0046872 GO:0047429 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0072529 GO:0090407 GO:1901135 GO:1901136 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576
PFAM: PF00692.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55687.t1	RHN67997.1	98.7	150	2	0	1	150	38	187	1.00E-74	289.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55687.t1	Q9STG6	87.9	141	17	0	10	150	26	166	1.7e-65	250.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55687.t1	A0A072UXL1	98.7	150	2	0	1	150	1	150	7.5e-75	289.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049397	MS.gene55687	0.824129	9.54E-54	-1.69E-46
MS.gene049622	MS.gene55687	0.818758	1.66E-52	-1.69E-46
MS.gene04985	MS.gene55687	0.800073	1.71E-48	-1.69E-46
MS.gene049989	MS.gene55687	0.859911	2.98E-63	-1.69E-46
MS.gene050297	MS.gene55687	0.885084	1.10E-71	-1.69E-46
MS.gene050648	MS.gene55687	0.848575	5.62E-60	-1.69E-46
MS.gene051094	MS.gene55687	0.809998	1.43E-50	-1.69E-46
MS.gene051263	MS.gene55687	0.855547	5.86E-62	-1.69E-46
MS.gene051478	MS.gene55687	0.817621	3.00E-52	-1.69E-46
MS.gene051629	MS.gene55687	0.83551	1.61E-56	-1.69E-46
MS.gene051816	MS.gene55687	0.800196	1.61E-48	-1.69E-46
MS.gene052367	MS.gene55687	0.869945	2.12E-66	-1.69E-46
MS.gene053416	MS.gene55687	0.813551	2.42E-51	-1.69E-46
MS.gene053417	MS.gene55687	0.815042	1.13E-51	-1.69E-46
MS.gene05369	MS.gene55687	0.808162	3.55E-50	-1.69E-46
MS.gene054643	MS.gene55687	0.815818	7.61E-52	-1.69E-46
MS.gene055578	MS.gene55687	0.814823	1.27E-51	-1.69E-46
MS.gene055957	MS.gene55687	0.883051	6.22E-71	-1.69E-46
MS.gene056041	MS.gene55687	0.858276	9.20E-63	-1.69E-46
MS.gene056042	MS.gene55687	0.809738	1.63E-50	-1.69E-46
MS.gene057458	MS.gene55687	0.81954	1.10E-52	-1.69E-46
MS.gene057483	MS.gene55687	0.82004	8.46E-53	-1.69E-46
MS.gene058196	MS.gene55687	0.846911	1.61E-59	-1.69E-46
MS.gene058197	MS.gene55687	0.8478	9.19E-60	-1.69E-46
MS.gene058198	MS.gene55687	0.851652	7.72E-61	-1.69E-46
MS.gene058818	MS.gene55687	0.806335	8.65E-50	-1.69E-46
MS.gene058844	MS.gene55687	0.812326	4.49E-51	-1.69E-46
MS.gene059300	MS.gene55687	0.85456	1.13E-61	-1.69E-46
MS.gene060473	MS.gene55687	0.844758	6.20E-59	-1.69E-46
MS.gene060565	MS.gene55687	0.837467	5.11E-57	-1.69E-46
MS.gene06057	MS.gene55687	0.85912	5.15E-63	-1.69E-46
MS.gene061260	MS.gene55687	0.877072	8.42E-69	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene55687	MS.gene55626	PPI
MS.gene55687	MS.gene82109	PPI
MS.gene79799	MS.gene55687	PPI
MS.gene55687	MS.gene47284	PPI
MS.gene55687	MS.gene042871	PPI
MS.gene55687	MS.gene066960	PPI
MS.gene55687	MS.gene49838	PPI
MS.gene87898	MS.gene55687	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55687.t1	MTR_3g063110	98.667	150	2	0	1	150	1	150	1.01e-103	293

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55687.t1	AT3G46940	87.943	141	17	0	10	150	26	166	5.46e-87	251
MS.gene55687.t1	AT3G46940	87.943	141	17	0	10	150	67	207	6.22e-87	253

Find 46 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTGAAGTTTCTGAGGTTT+TGG	0.179108	3.3:+57070010	MS.gene55687:CDS
GTTAGAGGTGAAGGTGGTTT+TGG	0.293840	3.3:+57070047	MS.gene55687:CDS
GATTTGGATTCTACTGTTAG+AGG	0.343621	3.3:+57070032	MS.gene55687:CDS
TTTCTTCACTCGGAGAAAAC+TGG	0.351836	3.3:-57069576	None:intergenic
ACACGAGCATAGGTTCCTTC+AGG	0.369361	3.3:-57069731	None:intergenic
AACCTTTGAATCGGCGGCGC+TGG	0.374420	3.3:-57069660	None:intergenic
TCAACAATGTCCAACTTAAA+CGG	0.388632	3.3:+57069542	None:intergenic
CTGATTACAGAGGACCTGTT+GGG	0.415838	3.3:+57069901	MS.gene55687:CDS
TCCAAAACCTCAGAAACTTC+AGG	0.420422	3.3:-57070011	None:intergenic
CATTCGATTGACGTTGGTGC+TGG	0.432456	3.3:+57069867	MS.gene55687:CDS
GGCGGAGAGAGGAGAGCCGC+GGG	0.455885	3.3:-57069624	None:intergenic
ATGAGAGCTTTGCCGCGAGC+AGG	0.461473	3.3:-57069683	None:intergenic
TGAAACGACGCCGTTTAAGT+TGG	0.465891	3.3:-57069552	None:intergenic
CGCCGAGATCGGGGTTGACA+TGG	0.473074	3.3:+57069841	MS.gene55687:CDS
TTCCATGTCAACCCCGATCT+CGG	0.481839	3.3:-57069843	None:intergenic
TTAAACAGTATAACCCCAAC+AGG	0.497604	3.3:-57069915	None:intergenic
CGCGGCTCTCCTCTCTCCGC+CGG	0.497937	3.3:+57069626	MS.gene55687:CDS
GGAAGGTATAACGGCTTTGT+CGG	0.505146	3.3:-57069603	None:intergenic
GATTCTACTGTTAGAGGTGA+AGG	0.505946	3.3:+57070038	MS.gene55687:CDS
TGTCGGAGAGTTTCTTCACT+CGG	0.507441	3.3:-57069586	None:intergenic
CTCCAGCGCCGCCGATTCAA+AGG	0.509909	3.3:+57069658	MS.gene55687:CDS
CTCTCCATCGCCGTCCCTGA+AGG	0.513836	3.3:+57069716	MS.gene55687:CDS
GGCGCTGGAGAGATCGTATC+CGG	0.519854	3.3:-57069645	None:intergenic
TATTACTCCTGAAGTTTCTG+AGG	0.531575	3.3:+57070004	MS.gene55687:CDS
CACGAGCATAGGTTCCTTCA+GGG	0.535140	3.3:-57069730	None:intergenic
GCGAGCAGGAACCTTTGAAT+CGG	0.540643	3.3:-57069669	None:intergenic
AGAGCCGCGGGAAGGTATAA+CGG	0.542038	3.3:-57069612	None:intergenic
ACGGCGATGGAGAGATCTGT+GGG	0.554559	3.3:-57069707	None:intergenic
GGTTCCTTCAGGGACGGCGA+TGG	0.559917	3.3:-57069720	None:intergenic
TCTACTGTTAGAGGTGAAGG+TGG	0.578300	3.3:+57070041	MS.gene55687:CDS
GACGGCGATGGAGAGATCTG+TGG	0.578622	3.3:-57069708	None:intergenic
AGCAGGAACCTTTGAATCGG+CGG	0.581168	3.3:-57069666	None:intergenic
GTAATTACCAACACGAGCAT+AGG	0.588762	3.3:-57069741	None:intergenic
AGATCGTATCCGGCGGAGAG+AGG	0.592423	3.3:-57069635	None:intergenic
GAGAGAGGAGAGCCGCGGGA+AGG	0.593764	3.3:-57069620	None:intergenic
GAAGGAACCTATGCTCGTGT+TGG	0.606547	3.3:+57069734	MS.gene55687:CDS
CGGCGGAGAGAGGAGAGCCG+CGG	0.611864	3.3:-57069625	None:intergenic
GCTGATTACAGAGGACCTGT+TGG	0.614644	3.3:+57069900	MS.gene55687:CDS
TGGAAGCATTCGATTGACGT+TGG	0.614719	3.3:+57069861	MS.gene55687:CDS
AGCATAGGTTCCTTCAGGGA+CGG	0.629807	3.3:-57069726	None:intergenic
GATTCAAAGGTTCCTGCTCG+CGG	0.632457	3.3:+57069671	MS.gene55687:CDS
TGATTACAGAGGACCTGTTG+GGG	0.654205	3.3:+57069902	MS.gene55687:CDS
AAAGCCGTTATACCTTCCCG+CGG	0.736048	3.3:+57069608	MS.gene55687:CDS
GTGATTGATGCTGATTACAG+AGG	0.740502	3.3:+57069891	MS.gene55687:CDS
GCTGGAGAGATCGTATCCGG+CGG	0.743244	3.3:-57069642	None:intergenic
CGGCGATGGAGAGATCTGTG+GGG	0.756952	3.3:-57069706	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAACCCTAAAATTCAAAAT+TGG	-	chr3.3:57069781-57069800	None:intergenic	20.0%
!!	AAACCCTAAAATTCAAAATT+GGG	-	chr3.3:57069780-57069799	None:intergenic	20.0%
!!	TAAAATTCAAAATTGGGAAA+GGG	-	chr3.3:57069774-57069793	None:intergenic	20.0%
!!!	ATTTTAGGGTTTTGAAATTT+AGG	+	chr3.3:57069788-57069807	MS.gene55687:intron	20.0%
!!!	TTTCCCAATTTTGAATTTTA+GGG	+	chr3.3:57069774-57069793	MS.gene55687:intron	20.0%
!!!	TTTTAGGGTTTTGAAATTTA+GGG	+	chr3.3:57069789-57069808	MS.gene55687:intron	20.0%
!	AAAATTCAAAATTGGGAAAG+GGG	-	chr3.3:57069773-57069792	None:intergenic	25.0%
!	CTAAAATTCAAAATTGGGAA+AGG	-	chr3.3:57069775-57069794	None:intergenic	25.0%
!!	CTTTCCCAATTTTGAATTTT+AGG	+	chr3.3:57069773-57069792	MS.gene55687:intron	25.0%
!!!	GTTGATTTTGAAGTGAAAGT+TGG	+	chr3.3:57069948-57069967	MS.gene55687:CDS	30.0%
	GATTTGGATTCTACTGTTAG+AGG	+	chr3.3:57070032-57070051	MS.gene55687:CDS	35.0%
	TATTACTCCTGAAGTTTCTG+AGG	+	chr3.3:57070004-57070023	MS.gene55687:CDS	35.0%
	TTAAACAGTATAACCCCAAC+AGG	-	chr3.3:57069918-57069937	None:intergenic	35.0%
!!!	AGTTTCTGAGGTTTTGGATT+TGG	+	chr3.3:57070016-57070035	MS.gene55687:CDS	35.0%
	GATTCTACTGTTAGAGGTGA+AGG	+	chr3.3:57070038-57070057	MS.gene55687:CDS	40.0%
	GTAATTACCAACACGAGCAT+AGG	-	chr3.3:57069744-57069763	None:intergenic	40.0%
	TCCAAAACCTCAGAAACTTC+AGG	-	chr3.3:57070014-57070033	None:intergenic	40.0%
	TTTCTTCACTCGGAGAAAAC+TGG	-	chr3.3:57069579-57069598	None:intergenic	40.0%
!	GTGATTGATGCTGATTACAG+AGG	+	chr3.3:57069891-57069910	MS.gene55687:CDS	40.0%
!!!	AAGGTGGTTTTGGATCTACT+GGG	+	chr3.3:57070057-57070076	MS.gene55687:CDS	40.0%
!!!	TCCTGAAGTTTCTGAGGTTT+TGG	+	chr3.3:57070010-57070029	MS.gene55687:CDS	40.0%
	CTGATTACAGAGGACCTGTT+GGG	+	chr3.3:57069901-57069920	MS.gene55687:CDS	45.0%
	TCTACTGTTAGAGGTGAAGG+TGG	+	chr3.3:57070041-57070060	MS.gene55687:CDS	45.0%
	TGAAACGACGCCGTTTAAGT+TGG	-	chr3.3:57069555-57069574	None:intergenic	45.0%
	TGATTACAGAGGACCTGTTG+GGG	+	chr3.3:57069902-57069921	MS.gene55687:CDS	45.0%
	TGTCGGAGAGTTTCTTCACT+CGG	-	chr3.3:57069589-57069608	None:intergenic	45.0%
!	GGAAGGTATAACGGCTTTGT+CGG	-	chr3.3:57069606-57069625	None:intergenic	45.0%
!	GTTAGAGGTGAAGGTGGTTT+TGG	+	chr3.3:57070047-57070066	MS.gene55687:CDS	45.0%
!!	TGGAAGCATTCGATTGACGT+TGG	+	chr3.3:57069861-57069880	MS.gene55687:CDS	45.0%
!!!	AGGTGGTTTTGGATCTACTG+GGG	+	chr3.3:57070058-57070077	MS.gene55687:CDS	45.0%
!!!	GAAGGTGGTTTTGGATCTAC+TGG	+	chr3.3:57070056-57070075	MS.gene55687:CDS	45.0%
	AAAGCCGTTATACCTTCCCG+CGG	+	chr3.3:57069608-57069627	MS.gene55687:CDS	50.0%
	ACACGAGCATAGGTTCCTTC+AGG	-	chr3.3:57069734-57069753	None:intergenic	50.0%
	AGCAGGAACCTTTGAATCGG+CGG	-	chr3.3:57069669-57069688	None:intergenic	50.0%
	AGCATAGGTTCCTTCAGGGA+CGG	-	chr3.3:57069729-57069748	None:intergenic	50.0%
	CACGAGCATAGGTTCCTTCA+GGG	-	chr3.3:57069733-57069752	None:intergenic	50.0%
	CATTCGATTGACGTTGGTGC+TGG	+	chr3.3:57069867-57069886	MS.gene55687:CDS	50.0%
	GAAGGAACCTATGCTCGTGT+TGG	+	chr3.3:57069734-57069753	MS.gene55687:CDS	50.0%
	GATTCAAAGGTTCCTGCTCG+CGG	+	chr3.3:57069671-57069690	MS.gene55687:CDS	50.0%
	GCGAGCAGGAACCTTTGAAT+CGG	-	chr3.3:57069672-57069691	None:intergenic	50.0%
	GCTGATTACAGAGGACCTGT+TGG	+	chr3.3:57069900-57069919	MS.gene55687:CDS	50.0%
	TTCCATGTCAACCCCGATCT+CGG	-	chr3.3:57069846-57069865	None:intergenic	50.0%
!!	ATTTTTGCAGCGCCGAGATC+GGG	+	chr3.3:57069831-57069850	MS.gene55687:intron	50.0%
!!	GATTTTTGCAGCGCCGAGAT+CGG	+	chr3.3:57069830-57069849	MS.gene55687:intron	50.0%
	ACGGCGATGGAGAGATCTGT+GGG	-	chr3.3:57069710-57069729	None:intergenic	55.0%
!	AGAGCCGCGGGAAGGTATAA+CGG	-	chr3.3:57069615-57069634	None:intergenic	55.0%
!!	TTTTTGCAGCGCCGAGATCG+GGG	+	chr3.3:57069832-57069851	MS.gene55687:intron	55.0%
	AACCTTTGAATCGGCGGCGC+TGG	-	chr3.3:57069663-57069682	None:intergenic	60.0%
	AGATCGTATCCGGCGGAGAG+AGG	-	chr3.3:57069638-57069657	None:intergenic	60.0%
	ATGAGAGCTTTGCCGCGAGC+AGG	-	chr3.3:57069686-57069705	None:intergenic	60.0%
	CGGCGATGGAGAGATCTGTG+GGG	-	chr3.3:57069709-57069728	None:intergenic	60.0%
	GACGGCGATGGAGAGATCTG+TGG	-	chr3.3:57069711-57069730	None:intergenic	60.0%
	GCTGGAGAGATCGTATCCGG+CGG	-	chr3.3:57069645-57069664	None:intergenic	60.0%
!	GGCGCTGGAGAGATCGTATC+CGG	-	chr3.3:57069648-57069667	None:intergenic	60.0%
	CGCCGAGATCGGGGTTGACA+TGG	+	chr3.3:57069841-57069860	MS.gene55687:CDS	65.0%
	CTCTCCATCGCCGTCCCTGA+AGG	+	chr3.3:57069716-57069735	MS.gene55687:CDS	65.0%
	GGTTCCTTCAGGGACGGCGA+TGG	-	chr3.3:57069723-57069742	None:intergenic	65.0%
!!	CTCCAGCGCCGCCGATTCAA+AGG	+	chr3.3:57069658-57069677	MS.gene55687:CDS	65.0%
	GAGAGAGGAGAGCCGCGGGA+AGG	-	chr3.3:57069623-57069642	None:intergenic	70.0%
	CGCGGCTCTCCTCTCTCCGC+CGG	+	chr3.3:57069626-57069645	MS.gene55687:CDS	75.0%
	CGGCGGAGAGAGGAGAGCCG+CGG	-	chr3.3:57069628-57069647	None:intergenic	75.0%
	GGCGGAGAGAGGAGAGCCGC+GGG	-	chr3.3:57069627-57069646	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	57069548	57070085	57069548	ID=MS.gene55687
chr3.3	mRNA	57069548	57070085	57069548	ID=MS.gene55687.t1;Parent=MS.gene55687
chr3.3	exon	57069548	57069755	57069548	ID=MS.gene55687.t1.exon1;Parent=MS.gene55687.t1
chr3.3	CDS	57069548	57069755	57069548	ID=cds.MS.gene55687.t1;Parent=MS.gene55687.t1
chr3.3	exon	57069841	57070085	57069841	ID=MS.gene55687.t1.exon2;Parent=MS.gene55687.t1
chr3.3	CDS	57069841	57070085	57069841	ID=cds.MS.gene55687.t1;Parent=MS.gene55687.t1

Gene Sequence

>MS.gene55687

ATGTCCAACTTAAACGGCGTCGTTTCATCCAGTTTTCTCCGAGTGAAGAAACTCTCCGACAAAGCCGTTATACCTTCCCGCGGCTCTCCTCTCTCCGCCGGATACGATCTCTCCAGCGCCGCCGATTCAAAGGTTCCTGCTCGCGGCAAAGCTCTCATCCCCACAGATCTCTCCATCGCCGTCCCTGAAGGAACCTATGCTCGTGTTGGTAATTACTGTTTTCCCCTTTCCCAATTTTGAATTTTAGGGTTTTGAAATTTAGGGTTTTTGATTGAATTGAATGATTTTTGCAGCGCCGAGATCGGGGTTGACATGGAAGCATTCGATTGACGTTGGTGCTGGAGTGATTGATGCTGATTACAGAGGACCTGTTGGGGTTATACTGTTTAATCATTCAGATGTTGATTTTGAAGTGAAAGTTGGTGATAGAGTTGCTCAGTTGATTATTGAGAAGATTATTACTCCTGAAGTTTCTGAGGTTTTGGATTTGGATTCTACTGTTAGAGGTGAAGGTGGTTTTGGATCTACTGGGGTTTGA

Protein sequence

>MS.gene55687.t1

MSNLNGVVSSSFLRVKKLSDKAVIPSRGSPLSAGYDLSSAADSKVPARGKALIPTDLSIAVPEGTYARVAPRSGLTWKHSIDVGAGVIDADYRGPVGVILFNHSDVDFEVKVGDRVAQLIIEKIITPEVSEVLDLDSTVRGEGGFGSTGV