AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59466


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59466.t1 MTR_5g022010 92.784 291 19 2 1 291 1 289 0.0 500
MS.gene59466.t1 MTR_8g075950 51.143 350 96 14 1 286 1 339 5.93e-73 228
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59466.t1 AT5G66230 39.193 347 130 14 1 284 1 329 2.97e-45 156
MS.gene59466.t1 AT5G66230 38.617 347 132 14 1 284 217 545 4.11e-42 152
MS.gene59466.t1 AT3G51230 44.954 109 39 3 1 91 1 106 5.79e-18 80.5

Find 59 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAAATTCAGAGTCTTTC+TGG 0.117151 5.4:-13653821 MS.gene59466:CDS
AAACGAAGATGTTTCCCTTT+TGG 0.292026 5.4:+13652784 None:intergenic
TCACCAATTGTGGGTCTTGC+TGG 0.314090 5.4:-13653971 MS.gene59466:CDS
GTGAGAGTTGTTGTGGATGA+TGG 0.320301 5.4:+13654249 None:intergenic
ATGATGGCAGGTGGTGAAAA+TGG 0.338531 5.4:-13653256 MS.gene59466:intron
TCAAGATCGATGTGCATTGA+TGG 0.354068 5.4:-13654008 MS.gene59466:CDS
CTCTGAGAAGTCAAGGGACT+TGG 0.360313 5.4:+13653197 None:intergenic
CCAGAAAGACTCTGAATTTG+AGG 0.370802 5.4:+13653821 None:intergenic
TAGATGAAGATGCTTCTATC+TGG 0.397277 5.4:-13653107 MS.gene59466:CDS
CTTCTTCAACCATCTGCATA+AGG 0.410420 5.4:+13653852 None:intergenic
ACCTAATGTGTTGCATTTGA+AGG 0.412380 5.4:-13652818 MS.gene59466:CDS
AAGGTGGTTCCAAAATTTGC+TGG 0.416494 5.4:-13652919 MS.gene59466:CDS
TCAACAACAATCAATTTCTC+AGG 0.428450 5.4:-13653771 MS.gene59466:intron
TGAGAGTTGTTGTGGATGAT+GGG 0.430806 5.4:+13654250 None:intergenic
AAGGGATTACCAACACCAAA+AGG 0.433129 5.4:-13652799 MS.gene59466:CDS
CCCTTCAAATGCAACACATT+AGG 0.434003 5.4:+13652817 None:intergenic
GACAAATGATTCACCAATTG+TGG 0.435186 5.4:-13653981 MS.gene59466:CDS
TTCTTCAACCATCTGCATAA+GGG 0.467939 5.4:+13653853 None:intergenic
AGCAAAGCTTCACCAGATCC+TGG 0.479015 5.4:+13653893 None:intergenic
CCACCAGCAAGACCCACAAT+TGG 0.497063 5.4:+13653968 None:intergenic
GTGAAGAACACACCAGGATC+TGG 0.501240 5.4:-13653905 MS.gene59466:CDS
CCTAATGTGTTGCATTTGAA+GGG 0.505604 5.4:-13652817 MS.gene59466:CDS
GAGATTTCTCTGAGAAGTCA+AGG 0.515939 5.4:+13653190 None:intergenic
TATGCAGATGGTTGAAGAAG+AGG 0.527703 5.4:-13653849 MS.gene59466:CDS
AGATTCTGTGTCAAAATCAA+AGG 0.534978 5.4:-13654041 MS.gene59466:CDS
GATTCTGTGTCAAAATCAAA+GGG 0.549781 5.4:-13654040 MS.gene59466:CDS
CTACCTCTTTGTTTCGCAGA+CGG 0.551296 5.4:+13653932 None:intergenic
GTGACAGAAGGAAGTGTGGT+TGG 0.552956 5.4:-13653141 MS.gene59466:CDS
ATCTGGTGAAGCTTTGCTGA+GGG 0.555103 5.4:-13653888 MS.gene59466:CDS
GAGAGTTGTTGTGGATGATG+GGG 0.555778 5.4:+13654251 None:intergenic
CAACATTAGAGTGAATGAAA+AGG 0.556085 5.4:-13652938 MS.gene59466:CDS
AGATTTCTCTGAGAAGTCAA+GGG 0.560895 5.4:+13653191 None:intergenic
GGTGTTGGTGAGAGTTGTTG+TGG 0.563322 5.4:+13654242 None:intergenic
CCAATTGTGGGTCTTGCTGG+TGG 0.574159 5.4:-13653968 MS.gene59466:CDS
TTTCATATTCACAGCTACCT+TGG 0.574412 5.4:+13653232 None:intergenic
GGCTTGATGAACTGTGTGAA+GGG 0.581098 5.4:-13652966 MS.gene59466:CDS
AGGGATTACCAACACCAAAA+GGG 0.583936 5.4:-13652798 MS.gene59466:CDS
TGATGAACTTGTGAATGAAG+TGG 0.585794 5.4:-13652866 MS.gene59466:CDS
ACACCGTCTGCGAAACAAAG+AGG 0.595726 5.4:-13653935 MS.gene59466:CDS
ACAAATGATTCACCAATTGT+GGG 0.597784 5.4:-13653980 MS.gene59466:CDS
CAACAATGATGAAGATGATG+TGG 0.602585 5.4:-13653064 MS.gene59466:CDS
AGTGAAGACCCTTATGCAGA+TGG 0.608040 5.4:-13653861 MS.gene59466:CDS
TGTGGAGGAAGAAATAGCTG+AGG 0.610254 5.4:-13653046 MS.gene59466:CDS
GGGCTTGATGAACTGTGTGA+AGG 0.612048 5.4:-13652967 MS.gene59466:CDS
CATTAGAGTGAATGAAAAGG+TGG 0.614379 5.4:-13652935 MS.gene59466:CDS
TGCACCTCTGAGGTGACAGA+AGG 0.618247 5.4:-13653153 MS.gene59466:CDS
TTACTGACTTGAAAGTGGAA+TGG 0.620132 5.4:+13654221 None:intergenic
ATCCTGGTGTGTTCTTCACA+CGG 0.624024 5.4:+13653909 None:intergenic
CAATGATGAAGATGATGTGG+AGG 0.630227 5.4:-13653061 MS.gene59466:CDS
ACTTCCTTCTGTCACCTCAG+AGG 0.635704 5.4:+13653149 None:intergenic
ACTTGAAAGTGGAATGGTGT+TGG 0.644715 5.4:+13654227 None:intergenic
GATCTGGTGAAGCTTTGCTG+AGG 0.645257 5.4:-13653889 MS.gene59466:CDS
AGCCGTGTGAAGAACACACC+AGG 0.662563 5.4:-13653911 MS.gene59466:CDS
ATCAGAAGAATGCACCTCTG+AGG 0.671388 5.4:-13653163 MS.gene59466:CDS
TCTGGTGAAGCTTTGCTGAG+GGG 0.673835 5.4:-13653887 MS.gene59466:CDS
AATTTGCTGGAAAACACACA+AGG 0.675885 5.4:-13652906 MS.gene59466:CDS
TGAACTTGTGAATGAAGTGG+AGG 0.693057 5.4:-13652863 MS.gene59466:CDS
TGAGGTGACAGAAGGAAGTG+TGG 0.713802 5.4:-13653145 MS.gene59466:CDS
CAGGTGGTGAAAATGGACCA+AGG 0.731969 5.4:-13653249 MS.gene59466:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTTTCTGAAATTTT+AGG - chr5.4:13653428-13653447 MS.gene59466:intron 10.0%
!! AAAAAGAAAAAGATTATGAA+GGG - chr5.4:13654018-13654037 MS.gene59466:CDS 15.0%
!! ACTTTATAGATTATATATCT+AGG + chr5.4:13653580-13653599 None:intergenic 15.0%
!!! ATTTTTTTCAAGATGTTTAT+TGG + chr5.4:13652869-13652888 None:intergenic 15.0%
!!! CATGCAAATTTATATTTTTA+TGG - chr5.4:13652825-13652844 MS.gene59466:CDS 15.0%
!! AAACATCTTGAAAAAAATTC+TGG - chr5.4:13652871-13652890 MS.gene59466:CDS 20.0%
!! AACATAAAGATTTCAATTAC+TGG + chr5.4:13653406-13653425 None:intergenic 20.0%
!! AACATCTTGAAAAAAATTCT+GGG - chr5.4:13652872-13652891 MS.gene59466:CDS 20.0%
!! ATAAGATCTCAATTTAATGA+TGG - chr5.4:13653733-13653752 MS.gene59466:intron 20.0%
!! GAAAAAGAAAAAGATTATGA+AGG - chr5.4:13654017-13654036 MS.gene59466:CDS 20.0%
!!! TCAATTGCTTTTGAAAAAAA+TGG - chr5.4:13653546-13653565 MS.gene59466:intron 20.0%
!!! TCTTGTTTTTTTTTTTTTGC+AGG - chr5.4:13652932-13652951 MS.gene59466:CDS 20.0%
!!! TTTTGAAAAAAATGGAGATT+TGG - chr5.4:13653554-13653573 MS.gene59466:intron 20.0%
! ACATGTGAGTTTCAATAAAA+TGG + chr5.4:13653374-13653393 None:intergenic 25.0%
! CACTTAATGAACTATTTATG+TGG - chr5.4:13653612-13653631 MS.gene59466:intron 25.0%
! CATAAATAGTTCATTAAGTG+AGG + chr5.4:13653612-13653631 None:intergenic 25.0%
! CATGTGAGTTTCAATAAAAT+GGG + chr5.4:13653373-13653392 None:intergenic 25.0%
!! GTATTTCATGCATTTTTCTT+TGG + chr5.4:13653278-13653297 None:intergenic 25.0%
!!! GCAAGTCTTTTTAATTAGTT+TGG - chr5.4:13653346-13653365 MS.gene59466:intron 25.0%
!!! TTTTTTTACTGACTTGAAAG+TGG + chr5.4:13652792-13652811 None:intergenic 25.0%
ACAAATGATTCACCAATTGT+GGG - chr5.4:13653025-13653044 MS.gene59466:CDS 30.0%
AGATTCTGTGTCAAAATCAA+AGG - chr5.4:13652964-13652983 MS.gene59466:CDS 30.0%
CAACATTAGAGTGAATGAAA+AGG - chr5.4:13654067-13654086 MS.gene59466:intron 30.0%
GATTCTGTGTCAAAATCAAA+GGG - chr5.4:13652965-13652984 MS.gene59466:CDS 30.0%
TCAACAACAATCAATTTCTC+AGG - chr5.4:13653234-13653253 MS.gene59466:CDS 30.0%
! AGTGATTGTATAAAGTGTCT+TGG - chr5.4:13653673-13653692 MS.gene59466:intron 30.0%
! CATGCATTTTTCTTTGGTTA+GGG + chr5.4:13653272-13653291 None:intergenic 30.0%
! TCATGCATTTTTCTTTGGTT+AGG + chr5.4:13653273-13653292 None:intergenic 30.0%
ACCTAATGTGTTGCATTTGA+AGG - chr5.4:13654187-13654206 MS.gene59466:intron 35.0%
CAACAATGATGAAGATGATG+TGG - chr5.4:13653941-13653960 MS.gene59466:CDS 35.0%
CATTAGAGTGAATGAAAAGG+TGG - chr5.4:13654070-13654089 MS.gene59466:intron 35.0%
CCTAATGTGTTGCATTTGAA+GGG - chr5.4:13654188-13654207 MS.gene59466:intron 35.0%
GACAAATGATTCACCAATTG+TGG - chr5.4:13653024-13653043 MS.gene59466:CDS 35.0%
GATCTCAATTTAATGATGGC+AGG - chr5.4:13653737-13653756 MS.gene59466:intron 35.0%
TAGATGAAGATGCTTCTATC+TGG - chr5.4:13653898-13653917 MS.gene59466:CDS 35.0%
TGATGAACTTGTGAATGAAG+TGG - chr5.4:13654139-13654158 MS.gene59466:intron 35.0%
TTCTTCAACCATCTGCATAA+GGG + chr5.4:13653155-13653174 None:intergenic 35.0%
TTTCATATTCACAGCTACCT+TGG + chr5.4:13653776-13653795 None:intergenic 35.0%
! AAACGAAGATGTTTCCCTTT+TGG + chr5.4:13654224-13654243 None:intergenic 35.0%
! AGATTTCTCTGAGAAGTCAA+GGG + chr5.4:13653817-13653836 None:intergenic 35.0%
! GTGTGTTTTCCAGCAAATTT+TGG + chr5.4:13654098-13654117 None:intergenic 35.0%
! TGTCTTGGTAGCATAATTTG+TGG - chr5.4:13653688-13653707 MS.gene59466:intron 35.0%
!! AATTTGCTGGAAAACACACA+AGG - chr5.4:13654099-13654118 MS.gene59466:intron 35.0%
!! GAAACATCTTCGTTTTTCTG+AGG - chr5.4:13654229-13654248 MS.gene59466:CDS 35.0%
!! TTACTGACTTGAAAGTGGAA+TGG + chr5.4:13652787-13652806 None:intergenic 35.0%
AAGGGATTACCAACACCAAA+AGG - chr5.4:13654206-13654225 MS.gene59466:intron 40.0%
AAGGTGGTTCCAAAATTTGC+TGG - chr5.4:13654086-13654105 MS.gene59466:intron 40.0%
ACTTGAAAGTGGAATGGTGT+TGG + chr5.4:13652781-13652800 None:intergenic 40.0%
AGGGATTACCAACACCAAAA+GGG - chr5.4:13654207-13654226 MS.gene59466:intron 40.0%
CAATGATGAAGATGATGTGG+AGG - chr5.4:13653944-13653963 MS.gene59466:CDS 40.0%
CCAGAAAGACTCTGAATTTG+AGG + chr5.4:13653187-13653206 None:intergenic 40.0%
CCCTTCAAATGCAACACATT+AGG + chr5.4:13654191-13654210 None:intergenic 40.0%
CCTCAAATTCAGAGTCTTTC+TGG - chr5.4:13653184-13653203 MS.gene59466:CDS 40.0%
CTCAATTTAATGATGGCAGG+TGG - chr5.4:13653740-13653759 MS.gene59466:intron 40.0%
CTTCTTCAACCATCTGCATA+AGG + chr5.4:13653156-13653175 None:intergenic 40.0%
TATGCAGATGGTTGAAGAAG+AGG - chr5.4:13653156-13653175 MS.gene59466:CDS 40.0%
TCAAGATCGATGTGCATTGA+TGG - chr5.4:13652997-13653016 MS.gene59466:CDS 40.0%
TGAACTTGTGAATGAAGTGG+AGG - chr5.4:13654142-13654161 MS.gene59466:intron 40.0%
TGAGAGTTGTTGTGGATGAT+GGG + chr5.4:13652758-13652777 None:intergenic 40.0%
! GAGATTTCTCTGAGAAGTCA+AGG + chr5.4:13653818-13653837 None:intergenic 40.0%
!! ACATCTTCGTTTTTCTGAGG+AGG - chr5.4:13654232-13654251 MS.gene59466:CDS 40.0%
!! AGATGTTTCCCTTTTGGTGT+TGG + chr5.4:13654218-13654237 None:intergenic 40.0%
AGTGAAGACCCTTATGCAGA+TGG - chr5.4:13653144-13653163 MS.gene59466:CDS 45.0%
ATCAGAAGAATGCACCTCTG+AGG - chr5.4:13653842-13653861 MS.gene59466:CDS 45.0%
ATCCTGGTGTGTTCTTCACA+CGG + chr5.4:13653099-13653118 None:intergenic 45.0%
ATCTGGTGAAGCTTTGCTGA+GGG - chr5.4:13653117-13653136 MS.gene59466:CDS 45.0%
ATGATGGCAGGTGGTGAAAA+TGG - chr5.4:13653749-13653768 MS.gene59466:intron 45.0%
GAGAGTTGTTGTGGATGATG+GGG + chr5.4:13652757-13652776 None:intergenic 45.0%
GGCTTGATGAACTGTGTGAA+GGG - chr5.4:13654039-13654058 MS.gene59466:CDS 45.0%
GTGAGAGTTGTTGTGGATGA+TGG + chr5.4:13652759-13652778 None:intergenic 45.0%
TGTGGAGGAAGAAATAGCTG+AGG - chr5.4:13653959-13653978 MS.gene59466:CDS 45.0%
! CTACCTCTTTGTTTCGCAGA+CGG + chr5.4:13653076-13653095 None:intergenic 45.0%
!! TCGTTTTTCTGAGGAGGAAG+AGG - chr5.4:13654238-13654257 MS.gene59466:CDS 45.0%
!! TTTTCTGAGGAGGAAGAGGA+AGG - chr5.4:13654242-13654261 MS.gene59466:CDS 45.0%
ACACCGTCTGCGAAACAAAG+AGG - chr5.4:13653070-13653089 MS.gene59466:CDS 50.0%
ACTTCCTTCTGTCACCTCAG+AGG + chr5.4:13653859-13653878 None:intergenic 50.0%
AGCAAAGCTTCACCAGATCC+TGG + chr5.4:13653115-13653134 None:intergenic 50.0%
CAGGTGGTGAAAATGGACCA+AGG - chr5.4:13653756-13653775 MS.gene59466:intron 50.0%
GATCTGGTGAAGCTTTGCTG+AGG - chr5.4:13653116-13653135 MS.gene59466:CDS 50.0%
GGGCTTGATGAACTGTGTGA+AGG - chr5.4:13654038-13654057 MS.gene59466:CDS 50.0%
GTGAAGAACACACCAGGATC+TGG - chr5.4:13653100-13653119 MS.gene59466:CDS 50.0%
TCACCAATTGTGGGTCTTGC+TGG - chr5.4:13653034-13653053 MS.gene59466:CDS 50.0%
TCTGGTGAAGCTTTGCTGAG+GGG - chr5.4:13653118-13653137 MS.gene59466:CDS 50.0%
TGAGGTGACAGAAGGAAGTG+TGG - chr5.4:13653860-13653879 MS.gene59466:CDS 50.0%
! CTCTGAGAAGTCAAGGGACT+TGG + chr5.4:13653811-13653830 None:intergenic 50.0%
! GTGACAGAAGGAAGTGTGGT+TGG - chr5.4:13653864-13653883 MS.gene59466:CDS 50.0%
!! GGTGTTGGTGAGAGTTGTTG+TGG + chr5.4:13652766-13652785 None:intergenic 50.0%
AGCCGTGTGAAGAACACACC+AGG - chr5.4:13653094-13653113 MS.gene59466:CDS 55.0%
CCAATTGTGGGTCTTGCTGG+TGG - chr5.4:13653037-13653056 MS.gene59466:CDS 55.0%
CCACCAGCAAGACCCACAAT+TGG + chr5.4:13653040-13653059 None:intergenic 55.0%
!! TGCACCTCTGAGGTGACAGA+AGG - chr5.4:13653852-13653871 MS.gene59466:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 13652747 13654280 13652747 ID=MS.gene59466
chr5.4 mRNA 13652747 13654280 13652747 ID=MS.gene59466.t1;Parent=MS.gene59466
chr5.4 exon 13654229 13654280 13654229 ID=MS.gene59466.t1.exon1;Parent=MS.gene59466.t1
chr5.4 CDS 13654229 13654280 13654229 ID=cds.MS.gene59466.t1;Parent=MS.gene59466.t1
chr5.4 exon 13653772 13654073 13653772 ID=MS.gene59466.t1.exon2;Parent=MS.gene59466.t1
chr5.4 CDS 13653772 13654073 13653772 ID=cds.MS.gene59466.t1;Parent=MS.gene59466.t1
chr5.4 exon 13652747 13653268 13652747 ID=MS.gene59466.t1.exon3;Parent=MS.gene59466.t1
chr5.4 CDS 13652747 13653268 13652747 ID=cds.MS.gene59466.t1;Parent=MS.gene59466.t1
Gene Sequence

>MS.gene59466

ATGGAAACCCCATCATCCACAACAACTCTCACCAACACCATTCCACTTTCAAGTCAGTAAAAAAAAATTCTCTCTTTTCATGCAAATTTATATTTTTATGGTTTAATTTCACTCTCAATCCAATAAACATCTTGAAAAAAATTCTGGGTTGTTCTAATATTCATGAATCAATAACATTTATGAATTCTTGTTTTTTTTTTTTTGCAGGAAAATTTGAAGATTCTGTGTCAAAATCAAAGGGTCAACAAAATCAAGATCGATGTGCATTGATGGACATGACAAATGATTCACCAATTGTGGGTCTTGCTGGTGGAAATCTCGAGACACCGTCTGCGAAACAAAGAGGTAGCCGTGTGAAGAACACACCAGGATCTGGTGAAGCTTTGCTGAGGGGACAAGTGAAGACCCTTATGCAGATGGTTGAAGAAGAGGCTTCTCCTCAAATTCAGAGTCTTTCTGGTAGTGTCAATAACAATGCTTCTGTTGTTCAACAACAATCAATTTCTCAGGTTCTTTTTTTATCCCTAACCAAAGAAAAATGCATGAAATACACTCTTTCTTTGTTTTCTTTTGTTTTGATATTTACTTTTGATTTTATTGCAAGTCTTTTTAATTAGTTTGGTCCCATTTTATTGAAACTCACATGTTCTTTGTCACCAGTAATTGAAATCTTTATGTTATTTATATTTTTCTGAAATTTTAGGTGTATAAAGATTAATATTTTTAGAATAGATATCATAATTAGTCATTTGTACAACTAAAATTTTATCATAGAACTCTTTATTTCTTTTGAGAAGTTTCAATTGCTTTTGAAAAAAATGGAGATTTGGCCTAGATATATAATCTATAAAGTTTGATTCATCCTCACTTAATGAACTATTTATGTGGCTTGATTAATCAATTTTTTTTTTATAACATCTCAATTTAGTGATTGTATAAAGTGTCTTGGTAGCATAATTTGTGGCTTGATTAATCAGTTTTTTTAAATAAGATCTCAATTTAATGATGGCAGGTGGTGAAAATGGACCAAGGTAGCTGTGAATATGAAAAGTGTGAGATAACCAAGTCCCTTGACTTCTCAGAGAAATCTCAAGTATCAGAAGAATGCACCTCTGAGGTGACAGAAGGAAGTGTGGTTGGTAGCTGCAGCATAGATGAAGATGCTTCTATCTGGTCAATGCAAGTCAACTCATGCAACAATGATGAAGATGATGTGGAGGAAGAAATAGCTGAGGAAGATGAAGAAGAAGAAGAAGACTACTATGATGTTGAAAAAGAAAAAGATTATGAAGGGCTTGATGAACTGTGTGAAGGGCTAAACAACATTAGAGTGAATGAAAAGGTGGTTCCAAAATTTGCTGGAAAACACACAAGGTTTGTTTACAACAGTGATGATGAACTTGTGAATGAAGTGGAGGATGAAAGTGTGAATGCTGATTCACCTAATGTGTTGCATTTGAAGGGATTACCAACACCAAAAGGGAAACATCTTCGTTTTTCTGAGGAGGAAGAGGAAGGAAAATCTGCTAAGTGA

Protein sequence

>MS.gene59466.t1

METPSSTTTLTNTIPLSRKFEDSVSKSKGQQNQDRCALMDMTNDSPIVGLAGGNLETPSAKQRGSRVKNTPGSGEALLRGQVKTLMQMVEEEASPQIQSLSGSVNNNASVVQQQSISQVVKMDQGSCEYEKCEITKSLDFSEKSQVSEECTSEVTEGSVVGSCSIDEDASIWSMQVNSCNNDEDDVEEEIAEEDEEEEEDYYDVEKEKDYEGLDELCEGLNNIRVNEKVVPKFAGKHTRFVYNSDDELVNEVEDESVNADSPNVLHLKGLPTPKGKHLRFSEEEEEGKSAK