AlfalfaGEDB | MS.gene61074

M. sativa cultivar XinJiangDaYe / MS.gene61074

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61074.t1	XP_003592080.1	77.7	184	9	1	1	152	1	184	6.00E-62	246.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61074.t1	G7IBX7	77.7	184	9	1	1	152	1	184	4.3e-62	246.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049825	MS.gene61074	0.824582	7.47E-54	-1.69E-46
MS.gene049873	MS.gene61074	0.805841	1.10E-49	-1.69E-46
MS.gene050277	MS.gene61074	0.81349	2.49E-51	-1.69E-46
MS.gene050303	MS.gene61074	0.849181	3.81E-60	-1.69E-46
MS.gene051633	MS.gene61074	0.808332	3.26E-50	-1.69E-46
MS.gene051700	MS.gene61074	0.804276	2.34E-49	-1.69E-46
MS.gene051746	MS.gene61074	0.804768	1.84E-49	-1.69E-46
MS.gene05188	MS.gene61074	0.820193	7.80E-53	-1.69E-46
MS.gene052309	MS.gene61074	-0.80049	1.41E-48	-1.69E-46
MS.gene052376	MS.gene61074	0.808968	2.39E-50	-1.69E-46
MS.gene055555	MS.gene61074	0.801949	7.07E-49	-1.69E-46
MS.gene055603	MS.gene61074	0.8248	6.63E-54	-1.69E-46
MS.gene055808	MS.gene61074	0.830347	3.09E-55	-1.69E-46
MS.gene056091	MS.gene61074	0.851416	9.01E-61	-1.69E-46
MS.gene056403	MS.gene61074	0.857376	1.70E-62	-1.69E-46
MS.gene056605	MS.gene61074	0.856886	2.37E-62	-1.69E-46
MS.gene056631	MS.gene61074	0.829118	6.15E-55	-1.69E-46
MS.gene05689	MS.gene61074	0.800967	1.12E-48	-1.69E-46
MS.gene056986	MS.gene61074	0.820978	5.15E-53	-1.69E-46
MS.gene057079	MS.gene61074	0.839593	1.44E-57	-1.69E-46
MS.gene058084	MS.gene61074	0.809135	2.20E-50	-1.69E-46
MS.gene058238	MS.gene61074	0.809085	2.25E-50	-1.69E-46
MS.gene058498	MS.gene61074	0.8144	1.57E-51	-1.69E-46
MS.gene058500	MS.gene61074	0.801186	1.01E-48	-1.69E-46
MS.gene058549	MS.gene61074	0.832105	1.14E-55	-1.69E-46
MS.gene05954	MS.gene61074	0.835017	2.14E-56	-1.69E-46
MS.gene05979	MS.gene61074	0.822848	1.90E-53	-1.69E-46
MS.gene059830	MS.gene61074	0.827562	1.46E-54	-1.69E-46
MS.gene059935	MS.gene61074	0.820669	6.07E-53	-1.69E-46
MS.gene06001	MS.gene61074	0.823509	1.33E-53	-1.69E-46
MS.gene06011	MS.gene61074	0.842782	2.10E-58	-1.69E-46
MS.gene060297	MS.gene61074	0.801602	8.33E-49	-1.69E-46
MS.gene060429	MS.gene61074	0.826231	3.04E-54	-1.69E-46
MS.gene060572	MS.gene61074	0.807656	4.54E-50	-1.69E-46
MS.gene060586	MS.gene61074	0.869305	3.42E-66	-1.69E-46
MS.gene060763	MS.gene61074	0.813395	2.62E-51	-1.69E-46
MS.gene060830	MS.gene61074	0.853824	1.85E-61	-1.69E-46
MS.gene060907	MS.gene61074	0.802077	6.66E-49	-1.69E-46
MS.gene061040	MS.gene61074	-0.804149	2.48E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61074.t1	MTR_1g098510	77.717	184	9	1	1	152	1	184	5.05e-94	270

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 42 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCTCCTGCTGCAGGCTTTA+TGG	0.092653	1.4:+76609869	MS.gene61074:CDS
CTCTTCTGCTACGGGCTTTA+TGG	0.095185	1.4:+76609698	MS.gene61074:CDS
ATAGATATAGAAAGCTTCTT+AGG	0.309898	1.4:+76610000	MS.gene61074:intron
TTCTGCCAAAGTGGTTGAAA+AGG	0.353945	1.4:+76610109	MS.gene61074:CDS
CTTGGTCCTCACCAGTTTCC+AGG	0.356142	1.4:-76609836	None:intergenic
TGCAGGCTTTATGGATTCAT+TGG	0.369416	1.4:+76609878	MS.gene61074:CDS
TGGATAAGTGCTTCAGAACT+TGG	0.383586	1.4:-76609645	None:intergenic
TTATGGATTCATTGGAAAAT+CGG	0.384322	1.4:+76609886	MS.gene61074:CDS
TGATTTGCCATGTCCTTGGC+TGG	0.408407	1.4:-76609768	None:intergenic
GTCGGTGCCCCTCCTGCTGC+AGG	0.419080	1.4:+76609861	MS.gene61074:CDS
CAGGAGGGGCACCGACTACT+TGG	0.421600	1.4:-76609854	None:intergenic
AAGTTCTGCAGCACCTATCC+TGG	0.422828	1.4:+76609818	MS.gene61074:CDS
AGGATAGGTGCTGCAGAACT+TGG	0.446859	1.4:-76609816	None:intergenic
AGTCGGTGGCTCTTCTGCTA+CGG	0.454743	1.4:+76609689	MS.gene61074:CDS
GCAGCACCTATCCTGGAAAC+TGG	0.465240	1.4:+76609825	MS.gene61074:CDS
GAAGCACTTATCCAGAAAAC+TGG	0.468163	1.4:+76609654	MS.gene61074:CDS
GTCGGTGGCTCTTCTGCTAC+GGG	0.474768	1.4:+76609690	MS.gene61074:CDS
TGGTTGATTTGCCATGTCCT+TGG	0.486730	1.4:-76609772	None:intergenic
ACTACGTGAGATGGTTGAAA+AGG	0.493284	1.4:+76610055	MS.gene61074:CDS
AGCTTCTTAGGACAGAGGAC+AGG	0.494425	1.4:+76610012	MS.gene61074:CDS
TCTGCCAAAGTGGTTGAAAA+GGG	0.502981	1.4:+76610110	MS.gene61074:CDS
TTCTAGTAATTCTGCCAAAG+TGG	0.522258	1.4:+76610100	MS.gene61074:CDS
ATGTTGCAATGTAGGCTACT+TGG	0.523428	1.4:-76610156	None:intergenic
CATAAAGCCTGCAGCAGGAG+GGG	0.525453	1.4:-76609868	None:intergenic
TCCTCACCAGTTTCCAGGAT+AGG	0.526596	1.4:-76609831	None:intergenic
TCCATAAAGCCTGCAGCAGG+AGG	0.530529	1.4:-76609870	None:intergenic
TTAGGACAGAGGACAGGGTA+CGG	0.537011	1.4:+76610018	MS.gene61074:CDS
GAATCCATAAAGCCTGCAGC+AGG	0.543830	1.4:-76609873	None:intergenic
TAGGACAGAGGACAGGGTAC+GGG	0.558112	1.4:+76610019	MS.gene61074:CDS
GGAAGGAATCTCAGAAGATG+TGG	0.565631	1.4:-76610135	None:intergenic
TCAAGCAAGACTACGTGAGA+TGG	0.592566	1.4:+76610046	MS.gene61074:CDS
CCATAAAGCCTGCAGCAGGA+GGG	0.618716	1.4:-76609869	None:intergenic
TAGCTGGAACACTCCAGCCA+AGG	0.621030	1.4:+76609755	MS.gene61074:CDS
GAACACTCCAGCCAAGGACA+TGG	0.636957	1.4:+76609761	MS.gene61074:CDS
GCTTCTTAGGACAGAGGACA+GGG	0.639564	1.4:+76610013	MS.gene61074:CDS
ACCTATCCTGGAAACTGGTG+AGG	0.641675	1.4:+76609830	MS.gene61074:CDS
TGCAATGTAGGCTACTTGGA+AGG	0.646398	1.4:-76610152	None:intergenic
ACTGGCGAGGATCAAGTAGT+CGG	0.654403	1.4:+76609672	MS.gene61074:CDS
TAGAAAGCTTCTTAGGACAG+AGG	0.663471	1.4:+76610007	MS.gene61074:CDS
ACTGGTGAGGACCAAGTAGT+CGG	0.664340	1.4:+76609843	MS.gene61074:CDS
GGCGAGGATCAAGTAGTCGG+TGG	0.665719	1.4:+76609675	MS.gene61074:CDS
ACTTATCCAGAAAACTGGCG+AGG	0.696112	1.4:+76609659	MS.gene61074:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATGGATTCATTGAAAAAT+CGG	+	chr1.4:76609715-76609734	MS.gene61074:CDS	20.0%
!	AAGAAGCTTTCTATATCTAT+AGG	-	chr1.4:76610000-76610019	None:intergenic	25.0%
!	AGAAACTTATCCTTATGTAT+GGG	+	chr1.4:76609932-76609951	MS.gene61074:intron	25.0%
!	ATAGATATAGAAAGCTTCTT+AGG	+	chr1.4:76610000-76610019	MS.gene61074:intron	25.0%
!	ATCAAAAAATCATAGATAGC+TGG	+	chr1.4:76609739-76609758	MS.gene61074:CDS	25.0%
!	TTATGGATTCATTGGAAAAT+CGG	+	chr1.4:76609886-76609905	MS.gene61074:CDS	25.0%
	CAGAAACTTATCCTTATGTA+TGG	+	chr1.4:76609931-76609950	MS.gene61074:intron	30.0%
	GAAACTTATCCTTATGTATG+GGG	+	chr1.4:76609933-76609952	MS.gene61074:intron	30.0%
	GGTTCCTATATTCTTGTATA+TGG	+	chr1.4:76609954-76609973	MS.gene61074:intron	30.0%
	TCCTATATTCTTGTATATGG+CGG	+	chr1.4:76609957-76609976	MS.gene61074:intron	30.0%
!!!	GCTTGTTGCTTTTCATTATT+TGG	-	chr1.4:76609795-76609814	None:intergenic	30.0%
	ATATAGGAACCCCATACATA+AGG	-	chr1.4:76609945-76609964	None:intergenic	35.0%
	TCCGCCATATACAAGAATAT+AGG	-	chr1.4:76609961-76609980	None:intergenic	35.0%
	TTCTAGTAATTCTGCCAAAG+TGG	+	chr1.4:76610100-76610119	MS.gene61074:CDS	35.0%
!!	ATTCTTGTATATGGCGGATT+TGG	+	chr1.4:76609963-76609982	MS.gene61074:intron	35.0%
!!!	GCTTGCTGCTTTTCATTATT+TGG	-	chr1.4:76609624-76609643	None:intergenic	35.0%
	ACTACGTGAGATGGTTGAAA+AGG	+	chr1.4:76610055-76610074	MS.gene61074:CDS	40.0%
	TAGAAAGCTTCTTAGGACAG+AGG	+	chr1.4:76610007-76610026	MS.gene61074:CDS	40.0%
	TCTGCCAAAGTGGTTGAAAA+GGG	+	chr1.4:76610110-76610129	MS.gene61074:CDS	40.0%
	TGGATAAGTGCTTCAGAACT+TGG	-	chr1.4:76609648-76609667	None:intergenic	40.0%
	TTCTGCCAAAGTGGTTGAAA+AGG	+	chr1.4:76610109-76610128	MS.gene61074:CDS	40.0%
!	GAAGCACTTATCCAGAAAAC+TGG	+	chr1.4:76609654-76609673	MS.gene61074:CDS	40.0%
!	TGCAGGCTTTATGGATTCAT+TGG	+	chr1.4:76609878-76609897	MS.gene61074:CDS	40.0%
!!	TATATGGCGGATTTGGTCTT+TGG	+	chr1.4:76609970-76609989	MS.gene61074:intron	40.0%
	ACTTATCCAGAAAACTGGCG+AGG	+	chr1.4:76609659-76609678	MS.gene61074:CDS	45.0%
	GGAAGGAATCTCAGAAGATG+TGG	-	chr1.4:76610138-76610157	None:intergenic	45.0%
	TCAAGCAAGACTACGTGAGA+TGG	+	chr1.4:76610046-76610065	MS.gene61074:CDS	45.0%
!	GGAACCCTTTTCAACCACTT+TGG	-	chr1.4:76610117-76610136	None:intergenic	45.0%
!!	TGGTTGATTTGCCATGTCCT+TGG	-	chr1.4:76609775-76609794	None:intergenic	45.0%
	AAGTTCTGCAGCACCTATCC+TGG	+	chr1.4:76609818-76609837	MS.gene61074:CDS	50.0%
	ACCTATCCTGGAAACTGGTG+AGG	+	chr1.4:76609830-76609849	MS.gene61074:CDS	50.0%
	ACTGGCGAGGATCAAGTAGT+CGG	+	chr1.4:76609672-76609691	MS.gene61074:CDS	50.0%
	ACTGGTGAGGACCAAGTAGT+CGG	+	chr1.4:76609843-76609862	MS.gene61074:CDS	50.0%
	AGCTTCTTAGGACAGAGGAC+AGG	+	chr1.4:76610012-76610031	MS.gene61074:CDS	50.0%
	CTCTTCTGCTACGGGCTTTA+TGG	+	chr1.4:76609698-76609717	MS.gene61074:CDS	50.0%
	GAATCCATAAAGCCTGCAGC+AGG	-	chr1.4:76609876-76609895	None:intergenic	50.0%
	GCTTCTTAGGACAGAGGACA+GGG	+	chr1.4:76610013-76610032	MS.gene61074:CDS	50.0%
	TCCTCACCAGTTTCCAGGAT+AGG	-	chr1.4:76609834-76609853	None:intergenic	50.0%
	TTAGGACAGAGGACAGGGTA+CGG	+	chr1.4:76610018-76610037	MS.gene61074:CDS	50.0%
!	AGGATAGGTGCTGCAGAACT+TGG	-	chr1.4:76609819-76609838	None:intergenic	50.0%
!	CTTGATCCTCGCCAGTTTTC+TGG	-	chr1.4:76609668-76609687	None:intergenic	50.0%
!	TGATTTGCCATGTCCTTGGC+TGG	-	chr1.4:76609771-76609790	None:intergenic	50.0%
	AGTCGGTGGCTCTTCTGCTA+CGG	+	chr1.4:76609689-76609708	MS.gene61074:CDS	55.0%
	CATAAAGCCTGCAGCAGGAG+GGG	-	chr1.4:76609871-76609890	None:intergenic	55.0%
	CCATAAAGCCTGCAGCAGGA+GGG	-	chr1.4:76609872-76609891	None:intergenic	55.0%
	CTTGGTCCTCACCAGTTTCC+AGG	-	chr1.4:76609839-76609858	None:intergenic	55.0%
	GAACACTCCAGCCAAGGACA+TGG	+	chr1.4:76609761-76609780	MS.gene61074:CDS	55.0%
	GCAGCACCTATCCTGGAAAC+TGG	+	chr1.4:76609825-76609844	MS.gene61074:CDS	55.0%
	TAGCTGGAACACTCCAGCCA+AGG	+	chr1.4:76609755-76609774	MS.gene61074:CDS	55.0%
	TAGGACAGAGGACAGGGTAC+GGG	+	chr1.4:76610019-76610038	MS.gene61074:CDS	55.0%
	TCCATAAAGCCTGCAGCAGG+AGG	-	chr1.4:76609873-76609892	None:intergenic	55.0%
	CCCTCCTGCTGCAGGCTTTA+TGG	+	chr1.4:76609869-76609888	MS.gene61074:CDS	60.0%
	GGCGAGGATCAAGTAGTCGG+TGG	+	chr1.4:76609675-76609694	MS.gene61074:CDS	60.0%
	GTCGGTGGCTCTTCTGCTAC+GGG	+	chr1.4:76609690-76609709	MS.gene61074:CDS	60.0%
!!	CAGGAGGGGCACCGACTACT+TGG	-	chr1.4:76609857-76609876	None:intergenic	65.0%
!	GTCGGTGCCCCTCCTGCTGC+AGG	+	chr1.4:76609861-76609880	MS.gene61074:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	76609609	76610163	76609609	ID=MS.gene61074
chr1.4	mRNA	76609609	76610163	76609609	ID=MS.gene61074.t1;Parent=MS.gene61074
chr1.4	exon	76609609	76609907	76609609	ID=MS.gene61074.t1.exon1;Parent=MS.gene61074.t1
chr1.4	CDS	76609609	76609907	76609609	ID=cds.MS.gene61074.t1;Parent=MS.gene61074.t1
chr1.4	exon	76610004	76610163	76610004	ID=MS.gene61074.t1.exon2;Parent=MS.gene61074.t1
chr1.4	CDS	76610004	76610163	76610004	ID=cds.MS.gene61074.t1;Parent=MS.gene61074.t1

Gene Sequence

>MS.gene61074

ATGGCAAATCAACCAAATAATGAAAAGCAGCAAGCACCAAGTTCTGAAGCACTTATCCAGAAAACTGGCGAGGATCAAGTAGTCGGTGGCTCTTCTGCTACGGGCTTTATGGATTCATTGAAAAATCGGTATCAAAAAATCATAGATAGCTGGAACACTCCAGCCAAGGACATGGCAAATCAACCAAATAATGAAAAGCAACAAGCACCAAGTTCTGCAGCACCTATCCTGGAAACTGGTGAGGACCAAGTAGTCGGTGCCCCTCCTGCTGCAGGCTTTATGGATTCATTGGAAAATCGGTATAAAAAAATCAAAGAGCATGCAGAAACTTATCCTTATGTATGGGGTTCCTATATTCTTGTATATGGCGGATTTGGTCTTTGGTTTGCCTATAGATATAGAAAGCTTCTTAGGACAGAGGACAGGGTACGGGCACTTCAAGCAAGACTACGTGAGATGGTTGAAAAGGATAAGATTGTTGAAGCTGAAAATTCTAGTAATTCTGCCAAAGTGGTTGAAAAGGGTTCCACATCTTCTGAGATTCCTTCCAAGTAG

Protein sequence

>MS.gene61074.t1

MANQPNNEKQQAPSSEALIQKTGEDQVVGGSSATGFMDSLKNRYQKIIDSWNTPAKDMANQPNNEKQQAPSSAAPILETGEDQVVGAPPAAGFMDSLENRYRKLLRTEDRVRALQARLREMVEKDKIVEAENSSNSAKVVEKGSTSSEIPSK