Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060907.t1 | XP_013465830.1 | 96.6 | 233 | 8 | 0 | 1 | 233 | 1 | 233 | 3.80E-124 | 454.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060907.t1 | O94675 | 24.8 | 218 | 122 | 7 | 50 | 232 | 59 | 269 | 2.6e-12 | 73.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060907.t1 | A0A072VE84 | 96.6 | 233 | 8 | 0 | 1 | 233 | 1 | 233 | 2.7e-124 | 454.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene060907 | 0.825822 | 3.80E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene060907 | -0.802427 | 5.64E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene060907 | 0.811184 | 7.95E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene060907 | 0.809163 | 2.17E-50 | -1.69E-46 |
| MS.gene050724 | MS.gene060907 | 0.804376 | 2.23E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene060907 | 0.806749 | 7.07E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene060907 | 0.824514 | 7.75E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene060907 | 0.810407 | 1.17E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene060907 | 0.815936 | 7.16E-52 | -1.69E-46 |
| MS.gene053054 | MS.gene060907 | -0.801028 | 1.09E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene060907 | 0.809605 | 1.74E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene060907 | 0.80154 | 8.58E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene060907 | 0.805682 | 1.19E-49 | -1.69E-46 |
| MS.gene056552 | MS.gene060907 | 0.800678 | 1.29E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene060907 | 0.805552 | 1.26E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene060907 | 0.818889 | 1.55E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene060907 | 0.802412 | 5.68E-49 | -1.69E-46 |
| MS.gene058548 | MS.gene060907 | 0.806057 | 9.89E-50 | -1.69E-46 |
| MS.gene058549 | MS.gene060907 | 0.818054 | 2.39E-52 | -1.69E-46 |
| MS.gene059249 | MS.gene060907 | 0.815997 | 6.94E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene060907 | 0.818611 | 1.79E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene060907 | 0.810291 | 1.24E-50 | -1.69E-46 |
| MS.gene05979 | MS.gene060907 | 0.816169 | 6.35E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene060907 | 0.82469 | 7.04E-54 | -1.69E-46 |
| MS.gene060572 | MS.gene060907 | 0.815676 | 8.18E-52 | -1.69E-46 |
| MS.gene060586 | MS.gene060907 | 0.806208 | 9.20E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene063917 | 0.821532 | 3.84E-53 | -1.69E-46 |
| MS.gene060907 | MS.gene064070 | 0.800373 | 1.49E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene064353 | 0.800482 | 1.41E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene066105 | 0.800825 | 1.20E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene066194 | 0.809225 | 2.10E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene066319 | 0.800894 | 1.16E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene066321 | 0.800198 | 1.61E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene067982 | 0.805102 | 1.57E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene068245 | 0.805954 | 1.04E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene068695 | 0.8005 | 1.40E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene069206 | 0.816887 | 4.39E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene069944 | 0.80759 | 4.69E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene07084 | 0.808971 | 2.38E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene071438 | -0.804974 | 1.67E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene071480 | 0.812148 | 4.91E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene071570 | 0.816645 | 4.97E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene072624 | 0.80772 | 4.40E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene072627 | 0.805437 | 1.34E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene072630 | 0.80569 | 1.18E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene07344 | 0.812138 | 4.93E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene073785 | 0.80014 | 1.66E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene073954 | 0.815428 | 9.29E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene074257 | 0.824511 | 7.76E-54 | -1.69E-46 |
| MS.gene060907 | MS.gene074527 | 0.803131 | 4.04E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene07798 | 0.802852 | 4.61E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene08618 | 0.801234 | 9.91E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene08853 | 0.814329 | 1.63E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene08882 | 0.806234 | 9.08E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene09952 | 0.805814 | 1.11E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene21149 | 0.817575 | 3.07E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene21200 | 0.813492 | 2.49E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene21928 | 0.823206 | 1.57E-53 | -1.69E-46 |
| MS.gene060907 | MS.gene22270 | 0.816264 | 6.05E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene22820 | -0.807211 | 5.65E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene23697 | 0.814363 | 1.60E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene24692 | 0.833648 | 4.73E-56 | -1.69E-46 |
| MS.gene060907 | MS.gene25207 | 0.803063 | 4.17E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene27991 | 0.803912 | 2.78E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene28218 | 0.825597 | 4.30E-54 | -1.69E-46 |
| MS.gene060907 | MS.gene28944 | 0.819201 | 1.32E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene29773 | 0.802007 | 6.88E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene30246 | 0.813289 | 2.76E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene30530 | 0.817526 | 3.15E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene30855 | 0.805465 | 1.32E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene30920 | 0.827884 | 1.22E-54 | -1.69E-46 |
| MS.gene060907 | MS.gene33160 | 0.918941 | 8.36E-87 | -1.69E-46 |
| MS.gene060907 | MS.gene34233 | 0.813627 | 2.33E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene34570 | 0.80147 | 8.87E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene35135 | 0.80811 | 3.64E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene35967 | 0.824686 | 7.06E-54 | -1.69E-46 |
| MS.gene060907 | MS.gene36281 | 0.806057 | 9.90E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene39572 | 0.817821 | 2.70E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene40513 | 0.822924 | 1.83E-53 | -1.69E-46 |
| MS.gene060907 | MS.gene41121 | 0.800187 | 1.62E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene43183 | 0.809284 | 2.04E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene43886 | 0.809159 | 2.17E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene43992 | 0.817538 | 3.13E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene45567 | 0.819666 | 1.03E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene47338 | 0.802124 | 6.51E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene47339 | 0.803804 | 2.93E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene47340 | 0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene47531 | 0.826358 | 2.84E-54 | -1.69E-46 |
| MS.gene060907 | MS.gene48244 | 0.802308 | 5.97E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene49486 | 0.813155 | 2.95E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene52103 | 0.802044 | 6.76E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene53383 | 0.804201 | 2.42E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene53579 | 0.811449 | 6.96E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene53586 | 0.807434 | 5.06E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene55397 | 0.806198 | 9.24E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene55540 | 0.816526 | 5.28E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene56253 | 0.800759 | 1.24E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene59506 | 0.817166 | 3.80E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene59769 | 0.802341 | 5.87E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene59949 | 0.801259 | 9.80E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene61074 | 0.802077 | 6.66E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene61183 | 0.919875 | 2.60E-87 | -1.69E-46 |
| MS.gene060907 | MS.gene62393 | 0.800944 | 1.14E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene62558 | 0.809143 | 2.19E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene64243 | 0.820783 | 5.72E-53 | -1.69E-46 |
| MS.gene060907 | MS.gene64249 | 0.805956 | 1.04E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene64650 | 0.814091 | 1.84E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene64807 | 0.815628 | 8.38E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene65650 | 0.823109 | 1.65E-53 | -1.69E-46 |
| MS.gene060907 | MS.gene66474 | 0.816791 | 4.61E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene68873 | 0.818999 | 1.46E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene69947 | 0.817548 | 3.11E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene71949 | 0.830626 | 2.64E-55 | -1.69E-46 |
| MS.gene060907 | MS.gene72203 | 0.802133 | 6.48E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene73877 | 0.801832 | 7.48E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene74076 | 0.806057 | 9.89E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene75003 | 0.800981 | 1.12E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene75605 | 0.808937 | 2.42E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene76035 | 0.816596 | 5.10E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene76403 | 0.811715 | 6.09E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene77453 | 0.800568 | 1.36E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene78492 | 0.801046 | 1.08E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene78659 | 0.825534 | 4.45E-54 | -1.69E-46 |
| MS.gene060907 | MS.gene79960 | 0.800293 | 1.54E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene81653 | 0.800074 | 1.71E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene81900 | 0.800598 | 1.34E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene81998 | 0.805522 | 1.28E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene82879 | 0.814575 | 1.44E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene83091 | 0.816844 | 4.48E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene85277 | 0.819097 | 1.39E-52 | -1.69E-46 |
| MS.gene060907 | MS.gene85663 | 0.801827 | 7.49E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene86086 | 0.808706 | 2.71E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene86380 | 0.808768 | 2.63E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene87285 | 0.808568 | 2.90E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene88243 | 0.806972 | 6.34E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene89562 | 0.801306 | 9.58E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene91907 | 0.804631 | 1.97E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene92188 | 0.806719 | 7.18E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene96322 | 0.802079 | 6.65E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene97439 | 0.807233 | 5.59E-50 | -1.69E-46 |
| MS.gene060907 | MS.gene98543 | 0.803779 | 2.96E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene99313 | 0.810524 | 1.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060907.t1 | MTR_1g013120 | 96.567 | 233 | 8 | 0 | 1 | 233 | 1 | 233 | 2.88e-170 | 468 |
| MS.gene060907.t1 | MTR_4g006560 | 43.069 | 202 | 106 | 6 | 36 | 233 | 40 | 236 | 2.15e-54 | 174 |
| MS.gene060907.t1 | MTR_7g063520 | 30.380 | 237 | 146 | 8 | 11 | 233 | 23 | 254 | 1.43e-21 | 90.1 |
| MS.gene060907.t1 | MTR_5g080880 | 31.381 | 239 | 146 | 9 | 4 | 232 | 24 | 254 | 1.01e-20 | 89.4 |
| MS.gene060907.t1 | MTR_3g007690 | 63.889 | 72 | 13 | 2 | 1 | 71 | 1 | 60 | 7.01e-20 | 85.5 |
| MS.gene060907.t1 | MTR_5g080870 | 29.143 | 175 | 98 | 7 | 61 | 232 | 68 | 219 | 2.82e-13 | 68.6 |
| MS.gene060907.t1 | MTR_5g093000 | 32.110 | 109 | 68 | 2 | 130 | 232 | 105 | 213 | 2.25e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060907.t1 | AT4G31930 | 54.468 | 235 | 104 | 2 | 1 | 233 | 1 | 234 | 1.36e-90 | 266 |
| MS.gene060907.t1 | AT1G80720 | 42.857 | 231 | 116 | 4 | 10 | 233 | 20 | 241 | 6.33e-61 | 191 |
| MS.gene060907.t1 | AT1G15870 | 42.667 | 225 | 117 | 6 | 17 | 233 | 21 | 241 | 1.86e-59 | 187 |
| MS.gene060907.t1 | AT1G80720 | 45.550 | 191 | 102 | 1 | 43 | 233 | 1 | 189 | 1.92e-59 | 186 |
| MS.gene060907.t1 | AT5G05990 | 29.924 | 264 | 147 | 11 | 1 | 232 | 1 | 258 | 2.47e-21 | 89.7 |
| MS.gene060907.t1 | AT5G02050 | 28.708 | 209 | 128 | 6 | 42 | 233 | 63 | 267 | 3.33e-21 | 89.4 |
| MS.gene060907.t1 | AT3G55605 | 30.488 | 164 | 103 | 4 | 79 | 233 | 97 | 258 | 1.17e-16 | 77.0 |
| MS.gene060907.t1 | AT4G32605 | 36.975 | 119 | 67 | 3 | 120 | 233 | 104 | 219 | 2.31e-15 | 72.8 |
| MS.gene060907.t1 | AT2G39795 | 27.315 | 216 | 132 | 9 | 33 | 233 | 45 | 250 | 1.33e-14 | 71.2 |
| MS.gene060907.t1 | AT2G39790 | 28.125 | 160 | 92 | 7 | 81 | 232 | 95 | 239 | 1.54e-12 | 65.5 |
Find 51 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTACCTTGGACCTGATTTC+AGG | 0.173345 | 1.2:-8538162 | MS.gene060907:intron |
| GTTACGATGAAGGGGAAATT+TGG | 0.184899 | 1.2:-8538415 | MS.gene060907:CDS |
| CAAAACTTCTCCTGAATCTT+AGG | 0.262004 | 1.2:+8537469 | None:intergenic |
| GTTGGATTCAAATGGAGATT+TGG | 0.307501 | 1.2:+8538787 | None:intergenic |
| GAGAGAACATTACTGATTCT+AGG | 0.315397 | 1.2:+8538857 | None:intergenic |
| TTACGATGAAGGGGAAATTT+GGG | 0.341454 | 1.2:-8538414 | MS.gene060907:CDS |
| AGGATAGAATTGAACTTCTA+AGG | 0.343544 | 1.2:-8537378 | MS.gene060907:CDS |
| CTCAGCGTGGCCTGATAGTT+TGG | 0.345831 | 1.2:-8538233 | MS.gene060907:CDS |
| ATTGAAGCTACAATGTTTGA+TGG | 0.356300 | 1.2:-8538373 | MS.gene060907:CDS |
| GAATCTGAAGCCTAAGATTC+AGG | 0.365605 | 1.2:-8537479 | MS.gene060907:CDS |
| TTTGAACATGTTCCTGCATA+TGG | 0.377388 | 1.2:-8538349 | MS.gene060907:CDS |
| GCATATGGAGATGATAGTTC+AGG | 0.394315 | 1.2:-8538334 | MS.gene060907:CDS |
| TTCTGAACATCCAAACTATC+AGG | 0.406918 | 1.2:+8538223 | None:intergenic |
| CGCAGAATGTTGGATTCAAA+TGG | 0.419260 | 1.2:+8538779 | None:intergenic |
| CATAATTCAAACCTGAAATC+AGG | 0.448439 | 1.2:+8538151 | None:intergenic |
| TGAATACGCTCCTCCTCATC+AGG | 0.457720 | 1.2:-8538726 | MS.gene060907:intron |
| AATGTTCTCTCTCAAACCTC+AGG | 0.465355 | 1.2:-8538845 | MS.gene060907:CDS |
| ACGGAGAATTCGCAGAATGT+TGG | 0.479523 | 1.2:+8538769 | None:intergenic |
| TCTTCATCTCAGTTTGATTG+TGG | 0.483776 | 1.2:-8538299 | MS.gene060907:CDS |
| ATCGTAACCACTTGCTCACC+AGG | 0.494801 | 1.2:+8538430 | None:intergenic |
| TTGAAGCTACAATGTTTGAT+GGG | 0.509177 | 1.2:-8538372 | MS.gene060907:CDS |
| TGAGCAAGTGGTTACGATGA+AGG | 0.510024 | 1.2:-8538425 | MS.gene060907:CDS |
| CTTGATACTCGATTTCGTTA+CGG | 0.514717 | 1.2:+8538750 | None:intergenic |
| CTGAAATCAGGTCCAAGGTA+AGG | 0.518651 | 1.2:+8538163 | None:intergenic |
| GAGCAAGTGGTTACGATGAA+GGG | 0.521381 | 1.2:-8538424 | MS.gene060907:CDS |
| TGATTGTGGATATATCGAAA+GGG | 0.531126 | 1.2:-8538285 | MS.gene060907:CDS |
| GAACTATCATCTCCATATGC+AGG | 0.542924 | 1.2:+8538337 | None:intergenic |
| CATATGGAGATGATAGTTCA+GGG | 0.543217 | 1.2:-8538333 | MS.gene060907:CDS |
| AACGATTTGAATTCTGTCAC+AGG | 0.543255 | 1.2:+8538469 | None:intergenic |
| TTGATTGTGGATATATCGAA+AGG | 0.546720 | 1.2:-8538286 | MS.gene060907:CDS |
| AATTGAACTTCTAAGGTGGA+TGG | 0.561187 | 1.2:-8537371 | MS.gene060907:CDS |
| TTGCGAGTACTTGGACGCAA+GGG | 0.561929 | 1.2:-8537449 | MS.gene060907:CDS |
| GATTGTGGATATATCGAAAG+GGG | 0.572237 | 1.2:-8538284 | MS.gene060907:CDS |
| GATATATCGAAAGGGGAAGG+TGG | 0.596404 | 1.2:-8538277 | MS.gene060907:CDS |
| AGAATAGTGGGAACCTGATG+AGG | 0.597183 | 1.2:+8538713 | None:intergenic |
| CAAACCTGAAATCAGGTCCA+AGG | 0.599008 | 1.2:+8538158 | None:intergenic |
| TTTGCGAGTACTTGGACGCA+AGG | 0.601738 | 1.2:-8537450 | MS.gene060907:CDS |
| ATAGAATTGAACTTCTAAGG+TGG | 0.601785 | 1.2:-8537375 | MS.gene060907:CDS |
| TGCGAGTACTTGGACGCAAG+GGG | 0.604395 | 1.2:-8537448 | MS.gene060907:CDS |
| AGCAAGTGGTTACGATGAAG+GGG | 0.615389 | 1.2:-8538423 | MS.gene060907:CDS |
| TTTATTGTTGAAGAGAGACC+TGG | 0.619462 | 1.2:-8538448 | MS.gene060907:CDS |
| AGAGAGACCTGGTGAGCAAG+TGG | 0.623326 | 1.2:-8538437 | MS.gene060907:CDS |
| TACGATGAAGGGGAAATTTG+GGG | 0.627192 | 1.2:-8538413 | MS.gene060907:CDS |
| ATGTCGACTAGGCCTTACCT+TGG | 0.627849 | 1.2:-8538175 | MS.gene060907:CDS |
| GTGGATATATCGAAAGGGGA+AGG | 0.628718 | 1.2:-8538280 | MS.gene060907:CDS |
| ATAGTGGGAACCTGATGAGG+AGG | 0.642607 | 1.2:+8538716 | None:intergenic |
| AGCGAGGGAAGATGTCGACT+AGG | 0.671718 | 1.2:-8538186 | MS.gene060907:CDS |
| AGCTCTGTTACTGAAACCTG+AGG | 0.676912 | 1.2:+8538829 | None:intergenic |
| ACACGAGTATATGATGAACA+AGG | 0.706429 | 1.2:-8537398 | MS.gene060907:CDS |
| TAGAATTCGTCTGCTCAGCG+TGG | 0.712888 | 1.2:-8538246 | MS.gene060907:CDS |
| ATATGGAGATGATAGTTCAG+GGG | 0.730665 | 1.2:-8538332 | MS.gene060907:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCTAAATCAAAAAAGTT+TGG | + | chr1.2:8538638-8538657 | None:intergenic | 15.0% |
| !!! | AAAAATAATTTTTTTCGAAG+TGG | - | chr1.2:8537705-8537724 | MS.gene060907:intron | 15.0% |
| !!! | AAAATAATTTTTTTCGAAGT+GGG | - | chr1.2:8537706-8537725 | MS.gene060907:intron | 15.0% |
| !!! | TTTGATCAATTTTGTTATTA+GGG | - | chr1.2:8537589-8537608 | MS.gene060907:intron | 15.0% |
| !!! | TTTTGATCAATTTTGTTATT+AGG | - | chr1.2:8537588-8537607 | MS.gene060907:intron | 15.0% |
| !! | AACATTTCAACTTTATTCAT+TGG | - | chr1.2:8538612-8538631 | MS.gene060907:intron | 20.0% |
| !! | TGAATAAAGTTGAAATGTTT+AGG | + | chr1.2:8538611-8538630 | None:intergenic | 20.0% |
| !!! | ATTTTTTTCATGTAGAATAG+TGG | + | chr1.2:8537562-8537581 | None:intergenic | 20.0% |
| !!! | TTTAAAACTTTGATAGCTTA+AGG | - | chr1.2:8538373-8538392 | MS.gene060907:CDS | 20.0% |
| !!! | TTTTCATCTGTTTAAATTGT+TGG | - | chr1.2:8538751-8538770 | MS.gene060907:CDS | 20.0% |
| !!! | TTTTTTTCATGTAGAATAGT+GGG | + | chr1.2:8537561-8537580 | None:intergenic | 20.0% |
| ! | AACAATCACATTGTGATATT+TGG | - | chr1.2:8538194-8538213 | MS.gene060907:CDS | 25.0% |
| ! | ATATCACAATGTGATTGTTA+TGG | + | chr1.2:8538193-8538212 | None:intergenic | 25.0% |
| !! | CAATTTTACTTACAAAAGCA+CGG | + | chr1.2:8538561-8538580 | None:intergenic | 25.0% |
| AGGATAGAATTGAACTTCTA+AGG | - | chr1.2:8538881-8538900 | MS.gene060907:CDS | 30.0% | |
| ATTGAAGCTACAATGTTTGA+TGG | - | chr1.2:8537886-8537905 | MS.gene060907:intron | 30.0% | |
| CATAATTCAAACCTGAAATC+AGG | + | chr1.2:8538111-8538130 | None:intergenic | 30.0% | |
| CATTGTGATATTTGGTATAG+TGG | - | chr1.2:8538202-8538221 | MS.gene060907:CDS | 30.0% | |
| CCAAACAACAAATGTACAAT+AGG | + | chr1.2:8538148-8538167 | None:intergenic | 30.0% | |
| TGATTGTGGATATATCGAAA+GGG | - | chr1.2:8537974-8537993 | MS.gene060907:intron | 30.0% | |
| TTGAAGCTACAATGTTTGAT+GGG | - | chr1.2:8537887-8537906 | MS.gene060907:intron | 30.0% | |
| TTGATTGTGGATATATCGAA+AGG | - | chr1.2:8537973-8537992 | MS.gene060907:intron | 30.0% | |
| ! | AAAGTTTTCATGTTAAAGCG+AGG | - | chr1.2:8538057-8538076 | MS.gene060907:intron | 30.0% |
| ! | AAGTTTTCATGTTAAAGCGA+GGG | - | chr1.2:8538058-8538077 | MS.gene060907:intron | 30.0% |
| ! | ATAGAATTGAACTTCTAAGG+TGG | - | chr1.2:8538884-8538903 | MS.gene060907:CDS | 30.0% |
| ! | CATTGCAAAACTTTTATCGA+AGG | - | chr1.2:8538682-8538701 | MS.gene060907:intron | 30.0% |
| ! | TGAAATGTTTAGGATCTGTT+AGG | + | chr1.2:8538601-8538620 | None:intergenic | 30.0% |
| !! | ATTGTACATTTGTTGTTTGG+TGG | - | chr1.2:8538148-8538167 | MS.gene060907:intron | 30.0% |
| !! | CCTATTGTACATTTGTTGTT+TGG | - | chr1.2:8538145-8538164 | MS.gene060907:intron | 30.0% |
| AACAAATGTACAATAGGCAC+AGG | + | chr1.2:8538142-8538161 | None:intergenic | 35.0% | |
| AACGATTTGAATTCTGTCAC+AGG | + | chr1.2:8537793-8537812 | None:intergenic | 35.0% | |
| AACTACGCAAAGACTGAATT+TGG | - | chr1.2:8538659-8538678 | MS.gene060907:intron | 35.0% | |
| ACACGAGTATATGATGAACA+AGG | - | chr1.2:8538861-8538880 | MS.gene060907:CDS | 35.0% | |
| ATATGGAGATGATAGTTCAG+GGG | - | chr1.2:8537927-8537946 | MS.gene060907:intron | 35.0% | |
| ATTTGATGCGTTAAGAGTTG+TGG | - | chr1.2:8538269-8538288 | MS.gene060907:CDS | 35.0% | |
| CAAAACTTCTCCTGAATCTT+AGG | + | chr1.2:8538793-8538812 | None:intergenic | 35.0% | |
| CAGTAACCAAAGTGAAAACA+AGG | + | chr1.2:8538465-8538484 | None:intergenic | 35.0% | |
| CATATGGAGATGATAGTTCA+GGG | - | chr1.2:8537926-8537945 | MS.gene060907:intron | 35.0% | |
| CTTGATACTCGATTTCGTTA+CGG | + | chr1.2:8537512-8537531 | None:intergenic | 35.0% | |
| GATTGTGGATATATCGAAAG+GGG | - | chr1.2:8537975-8537994 | MS.gene060907:intron | 35.0% | |
| GTTGGATTCAAATGGAGATT+TGG | + | chr1.2:8537475-8537494 | None:intergenic | 35.0% | |
| TCTTCATCTCAGTTTGATTG+TGG | - | chr1.2:8537960-8537979 | MS.gene060907:intron | 35.0% | |
| TTACGATGAAGGGGAAATTT+GGG | - | chr1.2:8537845-8537864 | MS.gene060907:intron | 35.0% | |
| TTCTGAACATCCAAACTATC+AGG | + | chr1.2:8538039-8538058 | None:intergenic | 35.0% | |
| TTTATTGTTGAAGAGAGACC+TGG | - | chr1.2:8537811-8537830 | MS.gene060907:intron | 35.0% | |
| TTTGAACATGTTCCTGCATA+TGG | - | chr1.2:8537910-8537929 | MS.gene060907:intron | 35.0% | |
| ! | CTGTTTAAATTGTTGGTTGC+AGG | - | chr1.2:8538758-8538777 | MS.gene060907:CDS | 35.0% |
| ! | TGATAGCTTAAGGATTTGCT+TGG | - | chr1.2:8538383-8538402 | MS.gene060907:CDS | 35.0% |
| !! | AATTGAACTTCTAAGGTGGA+TGG | - | chr1.2:8538888-8538907 | MS.gene060907:CDS | 35.0% |
| !! | GAGAGAACATTACTGATTCT+AGG | + | chr1.2:8537405-8537424 | None:intergenic | 35.0% |
| !! | TTGTTGTTTGGTGGAAATGA+AGG | - | chr1.2:8538157-8538176 | MS.gene060907:intron | 35.0% |
| !! | TTTTTCGAAGTGGGTAATTG+CGG | - | chr1.2:8537715-8537734 | MS.gene060907:intron | 35.0% |
| AATGTTCTCTCTCAAACCTC+AGG | - | chr1.2:8537414-8537433 | MS.gene060907:CDS | 40.0% | |
| CACAATTGCAGCTACATCAA+TGG | + | chr1.2:8538302-8538321 | None:intergenic | 40.0% | |
| CATTGATGTAGCTGCAATTG+TGG | - | chr1.2:8538300-8538319 | MS.gene060907:CDS | 40.0% | |
| CGCAGAATGTTGGATTCAAA+TGG | + | chr1.2:8537483-8537502 | None:intergenic | 40.0% | |
| GAACTATCATCTCCATATGC+AGG | + | chr1.2:8537925-8537944 | None:intergenic | 40.0% | |
| GCATATGGAGATGATAGTTC+AGG | - | chr1.2:8537925-8537944 | MS.gene060907:intron | 40.0% | |
| GTTACGATGAAGGGGAAATT+TGG | - | chr1.2:8537844-8537863 | MS.gene060907:intron | 40.0% | |
| TACGATGAAGGGGAAATTTG+GGG | - | chr1.2:8537846-8537865 | MS.gene060907:intron | 40.0% | |
| TGAATGCCTTCAAAACGACT+AGG | + | chr1.2:8538708-8538727 | None:intergenic | 40.0% | |
| ! | GAATCTGAAGCCTAAGATTC+AGG | - | chr1.2:8538780-8538799 | MS.gene060907:CDS | 40.0% |
| ! | GGATAGTCTCAAGTCTTTTG+TGG | - | chr1.2:8538909-8538928 | MS.gene060907:CDS | 40.0% |
| ! | TTTTCGAAGTGGGTAATTGC+GGG | - | chr1.2:8537716-8537735 | MS.gene060907:intron | 40.0% |
| !! | GTAATTGCGGGTTAGTGATT+TGG | - | chr1.2:8537728-8537747 | MS.gene060907:intron | 40.0% |
| !!! | ACGACACCTTGTTTTCACTT+TGG | - | chr1.2:8538456-8538475 | MS.gene060907:CDS | 40.0% |
| !!! | TCGTTTTGAAGGCATTCAGT+TGG | - | chr1.2:8538710-8538729 | MS.gene060907:intron | 40.0% |
| ACGGAGAATTCGCAGAATGT+TGG | + | chr1.2:8537493-8537512 | None:intergenic | 45.0% | |
| AGCAAGTGGTTACGATGAAG+GGG | - | chr1.2:8537836-8537855 | MS.gene060907:intron | 45.0% | |
| AGCTCTGTTACTGAAACCTG+AGG | + | chr1.2:8537433-8537452 | None:intergenic | 45.0% | |
| CAAACCTGAAATCAGGTCCA+AGG | + | chr1.2:8538104-8538123 | None:intergenic | 45.0% | |
| CGACTTTGCAGTCATAGTCA+AGG | - | chr1.2:8538344-8538363 | MS.gene060907:CDS | 45.0% | |
| CTGAAATCAGGTCCAAGGTA+AGG | + | chr1.2:8538099-8538118 | None:intergenic | 45.0% | |
| CTTACCTTGGACCTGATTTC+AGG | - | chr1.2:8538097-8538116 | MS.gene060907:intron | 45.0% | |
| GAGCAAGTGGTTACGATGAA+GGG | - | chr1.2:8537835-8537854 | MS.gene060907:intron | 45.0% | |
| GATATATCGAAAGGGGAAGG+TGG | - | chr1.2:8537982-8538001 | MS.gene060907:intron | 45.0% | |
| GTGGATATATCGAAAGGGGA+AGG | - | chr1.2:8537979-8537998 | MS.gene060907:intron | 45.0% | |
| TGAGCAAGTGGTTACGATGA+AGG | - | chr1.2:8537834-8537853 | MS.gene060907:intron | 45.0% | |
| ! | AGAATAGTGGGAACCTGATG+AGG | + | chr1.2:8537549-8537568 | None:intergenic | 45.0% |
| ! | GGAGAAGTTTTGCGAGTACT+TGG | - | chr1.2:8538801-8538820 | MS.gene060907:CDS | 45.0% |
| ATCGTAACCACTTGCTCACC+AGG | + | chr1.2:8537832-8537851 | None:intergenic | 50.0% | |
| TAGAATTCGTCTGCTCAGCG+TGG | - | chr1.2:8538013-8538032 | MS.gene060907:intron | 50.0% | |
| TGAATACGCTCCTCCTCATC+AGG | - | chr1.2:8537533-8537552 | MS.gene060907:intron | 50.0% | |
| TTGCGAGTACTTGGACGCAA+GGG | - | chr1.2:8538810-8538829 | MS.gene060907:CDS | 50.0% | |
| TTTGCGAGTACTTGGACGCA+AGG | - | chr1.2:8538809-8538828 | MS.gene060907:CDS | 50.0% | |
| ! | ATAGTGGGAACCTGATGAGG+AGG | + | chr1.2:8537546-8537565 | None:intergenic | 50.0% |
| ! | ATGTCGACTAGGCCTTACCT+TGG | - | chr1.2:8538084-8538103 | MS.gene060907:intron | 50.0% |
| !! | CGAAGGCCTAGTCGTTTTGA+AGG | - | chr1.2:8538699-8538718 | MS.gene060907:intron | 50.0% |
| AGAGAGACCTGGTGAGCAAG+TGG | - | chr1.2:8537822-8537841 | MS.gene060907:intron | 55.0% | |
| CTCAGCGTGGCCTGATAGTT+TGG | - | chr1.2:8538026-8538045 | MS.gene060907:intron | 55.0% | |
| GTAGCTGCAATTGTGGTCGC+AGG | - | chr1.2:8538307-8538326 | MS.gene060907:CDS | 55.0% | |
| TGCGAGTACTTGGACGCAAG+GGG | - | chr1.2:8538811-8538830 | MS.gene060907:CDS | 55.0% | |
| ! | AGCGAGGGAAGATGTCGACT+AGG | - | chr1.2:8538073-8538092 | MS.gene060907:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 8537342 | 8538939 | 8537342 | ID=MS.gene060907 |
| chr1.2 | mRNA | 8537342 | 8538939 | 8537342 | ID=MS.gene060907.t1;Parent=MS.gene060907 |
| chr1.2 | exon | 8538727 | 8538939 | 8538727 | ID=MS.gene060907.t1.exon1;Parent=MS.gene060907.t1 |
| chr1.2 | CDS | 8538727 | 8538939 | 8538727 | ID=cds.MS.gene060907.t1;Parent=MS.gene060907.t1 |
| chr1.2 | exon | 8538163 | 8538491 | 8538163 | ID=MS.gene060907.t1.exon2;Parent=MS.gene060907.t1 |
| chr1.2 | CDS | 8538163 | 8538491 | 8538163 | ID=cds.MS.gene060907.t1;Parent=MS.gene060907.t1 |
| chr1.2 | exon | 8537342 | 8537501 | 8537342 | ID=MS.gene060907.t1.exon3;Parent=MS.gene060907.t1 |
| chr1.2 | CDS | 8537342 | 8537501 | 8537342 | ID=cds.MS.gene060907.t1;Parent=MS.gene060907.t1 |
| Gene Sequence |
| Protein sequence |
