Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61226.t1 | RHN58490.1 | 84.1 | 208 | 33 | 0 | 1 | 208 | 58 | 265 | 1.40E-93 | 352.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61226.t1 | G7JGU4 | 84.1 | 208 | 33 | 0 | 1 | 208 | 1 | 208 | 1.0e-93 | 352.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene61226 | 0.810916 | 9.08E-51 | -1.69E-46 |
| MS.gene049350 | MS.gene61226 | 0.833926 | 4.03E-56 | -1.69E-46 |
| MS.gene049501 | MS.gene61226 | 0.801162 | 1.03E-48 | -1.69E-46 |
| MS.gene049711 | MS.gene61226 | 0.843648 | 1.23E-58 | -1.69E-46 |
| MS.gene049821 | MS.gene61226 | 0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene05045 | MS.gene61226 | 0.826737 | 2.30E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene61226 | 0.833203 | 6.10E-56 | -1.69E-46 |
| MS.gene050808 | MS.gene61226 | 0.8038 | 2.93E-49 | -1.69E-46 |
| MS.gene050901 | MS.gene61226 | 0.807787 | 4.26E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene61226 | 0.817072 | 3.99E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene61226 | 0.829366 | 5.36E-55 | -1.69E-46 |
| MS.gene051328 | MS.gene61226 | 0.806807 | 6.87E-50 | -1.69E-46 |
| MS.gene051388 | MS.gene61226 | 0.801895 | 7.25E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene61226 | 0.842134 | 3.11E-58 | -1.69E-46 |
| MS.gene051719 | MS.gene61226 | 0.869722 | 2.50E-66 | -1.69E-46 |
| MS.gene051720 | MS.gene61226 | 0.883507 | 4.23E-71 | -1.69E-46 |
| MS.gene051744 | MS.gene61226 | 0.812604 | 3.90E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene61226 | 0.822195 | 2.70E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene61226 | 0.812668 | 3.78E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene61226 | 0.814646 | 1.39E-51 | -1.69E-46 |
| MS.gene052732 | MS.gene61226 | 0.823254 | 1.53E-53 | -1.69E-46 |
| MS.gene053144 | MS.gene61226 | 0.853189 | 2.82E-61 | -1.69E-46 |
| MS.gene053171 | MS.gene61226 | 0.800962 | 1.13E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene61226 | 0.831573 | 1.55E-55 | -1.69E-46 |
| MS.gene053581 | MS.gene61226 | 0.836788 | 7.62E-57 | -1.69E-46 |
| MS.gene054584 | MS.gene61226 | 0.854488 | 1.19E-61 | -1.69E-46 |
| MS.gene054775 | MS.gene61226 | 0.829292 | 5.58E-55 | -1.69E-46 |
| MS.gene055144 | MS.gene61226 | 0.81263 | 3.85E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene61226 | 0.876897 | 9.69E-69 | -1.69E-46 |
| MS.gene055466 | MS.gene61226 | 0.867947 | 9.40E-66 | -1.69E-46 |
| MS.gene055467 | MS.gene61226 | 0.8096 | 1.75E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene61226 | 0.863207 | 2.94E-64 | -1.69E-46 |
| MS.gene055469 | MS.gene61226 | 0.845458 | 4.01E-59 | -1.69E-46 |
| MS.gene055537 | MS.gene61226 | 0.851198 | 1.04E-60 | -1.69E-46 |
| MS.gene055580 | MS.gene61226 | -0.839272 | 1.75E-57 | -1.69E-46 |
| MS.gene055778 | MS.gene61226 | 0.804526 | 2.07E-49 | -1.69E-46 |
| MS.gene056005 | MS.gene61226 | 0.819697 | 1.01E-52 | -1.69E-46 |
| MS.gene056043 | MS.gene61226 | -0.827472 | 1.54E-54 | -1.69E-46 |
| MS.gene056308 | MS.gene61226 | 0.833474 | 5.22E-56 | -1.69E-46 |
| MS.gene056595 | MS.gene61226 | 0.827707 | 1.35E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene61226 | 0.80543 | 1.34E-49 | -1.69E-46 |
| MS.gene058087 | MS.gene61226 | 0.820386 | 7.05E-53 | -1.69E-46 |
| MS.gene058339 | MS.gene61226 | 0.833823 | 4.27E-56 | -1.69E-46 |
| MS.gene05847 | MS.gene61226 | 0.816709 | 4.81E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene61226 | 0.806697 | 7.25E-50 | -1.69E-46 |
| MS.gene058668 | MS.gene61226 | 0.802373 | 5.79E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene61226 | 0.841994 | 3.39E-58 | -1.69E-46 |
| MS.gene059084 | MS.gene61226 | 0.801543 | 8.57E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene61226 | 0.822324 | 2.52E-53 | -1.69E-46 |
| MS.gene060429 | MS.gene61226 | 0.816797 | 4.59E-52 | -1.69E-46 |
| MS.gene060519 | MS.gene61226 | 0.828579 | 8.31E-55 | -1.69E-46 |
| MS.gene060603 | MS.gene61226 | 0.801542 | 8.57E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61226.t1 | MTR_4g009760 | 97.596 | 208 | 5 | 0 | 1 | 208 | 1 | 208 | 6.24e-156 | 430 |
| MS.gene61226.t1 | MTR_2g104230 | 60.000 | 220 | 65 | 10 | 1 | 208 | 1 | 209 | 5.87e-76 | 228 |
| MS.gene61226.t1 | MTR_4g116200 | 48.000 | 150 | 67 | 4 | 1 | 149 | 1 | 140 | 3.56e-35 | 125 |
| MS.gene61226.t1 | MTR_2g450720 | 61.290 | 93 | 33 | 2 | 1 | 92 | 1 | 91 | 3.90e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61226.t1 | AT1G15760 | 49.545 | 220 | 73 | 10 | 3 | 207 | 5 | 201 | 4.52e-59 | 184 |
| MS.gene61226.t1 | AT1G80520 | 50.463 | 216 | 73 | 10 | 1 | 207 | 1 | 191 | 2.49e-51 | 164 |
| MS.gene61226.t1 | AT2G12462 | 30.709 | 254 | 123 | 9 | 1 | 207 | 1 | 248 | 7.52e-27 | 103 |
Find 44 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATCTCTTGTATATGAGTA+TGG | 0.307188 | 4.2:-90407429 | MS.gene61226:CDS |
| CCATGCTTTGGAGAAATTCA+TGG | 0.323501 | 4.2:+90407355 | None:intergenic |
| GCTAGTAGTAGTAGTTATTA+TGG | 0.324043 | 4.2:-90407132 | MS.gene61226:CDS |
| AAACTTGCAAGAAAAGAAAA+AGG | 0.339805 | 4.2:-90407297 | MS.gene61226:CDS |
| GAGAATTTCTAGCCTGTGTT+TGG | 0.345241 | 4.2:+90407320 | None:intergenic |
| CTATGGAGATTCCCATGCTT+TGG | 0.347816 | 4.2:+90407343 | None:intergenic |
| ATGATCTTTGCCATTGGTCT+TGG | 0.351786 | 4.2:+90407261 | None:intergenic |
| ATCATGATAGCAATGGTTAT+TGG | 0.385458 | 4.2:-90406924 | MS.gene61226:CDS |
| CTACTACTAGCCCTTGTTGT+TGG | 0.387585 | 4.2:+90407144 | None:intergenic |
| CAACCATGGATTGGTTCTCT+TGG | 0.390859 | 4.2:-90407473 | None:intergenic |
| ATCTTTGCCATTGGTCTTGG+TGG | 0.398986 | 4.2:+90407264 | None:intergenic |
| CAGCTGCTGTTGAAGATATC+AGG | 0.407684 | 4.2:-90406900 | MS.gene61226:CDS |
| TTCTAGCCTGTGTTTGGCTA+TGG | 0.411531 | 4.2:+90407326 | None:intergenic |
| GCTCCCATGATCTTTGCCAT+TGG | 0.442339 | 4.2:+90407255 | None:intergenic |
| GAGAGGCTGTTGCTTACAAA+TGG | 0.465042 | 4.2:-90407045 | MS.gene61226:CDS |
| AAGACCAATGGCAAAGATCA+TGG | 0.469365 | 4.2:-90407259 | MS.gene61226:CDS |
| GCAGGCATCATTGAAGGACT+AGG | 0.479985 | 4.2:+90407018 | None:intergenic |
| GAAGAGGAATAAGAAGTTGA+AGG | 0.494849 | 4.2:-90407079 | MS.gene61226:CDS |
| TAACTACATAATGAGAAAGT+TGG | 0.500944 | 4.2:-90407205 | MS.gene61226:CDS |
| TCAACTTACCAACCATGGAT+TGG | 0.503114 | 4.2:-90407482 | None:intergenic |
| GCAAGAGCAGGCATCATTGA+AGG | 0.509469 | 4.2:+90407012 | None:intergenic |
| CATATACAAGAGATGGATCA+AGG | 0.528896 | 4.2:+90407436 | None:intergenic |
| TTTCATGAAGGAAAGATGAA+AGG | 0.538904 | 4.2:-90406952 | MS.gene61226:CDS |
| CCATGAATTTCTCCAAAGCA+TGG | 0.539592 | 4.2:-90407355 | MS.gene61226:CDS |
| CCATACTCATATACAAGAGA+TGG | 0.559337 | 4.2:+90407429 | None:intergenic |
| AGACCAATGGCAAAGATCAT+GGG | 0.562788 | 4.2:-90407258 | MS.gene61226:CDS |
| GAGGAATAAGAAGTTGAAGG+TGG | 0.566010 | 4.2:-90407076 | MS.gene61226:CDS |
| CATGAATTTCTCCAAAGCAT+GGG | 0.566734 | 4.2:-90407354 | MS.gene61226:CDS |
| TTGTTGTAGTTCCAACAACA+AGG | 0.568940 | 4.2:-90407155 | MS.gene61226:CDS |
| CAGGCATCATTGAAGGACTA+GGG | 0.582193 | 4.2:+90407019 | None:intergenic |
| GAGACAACCACCAAGACCAA+TGG | 0.584520 | 4.2:-90407271 | MS.gene61226:CDS |
| TCATGAAAATGATAAACCAT+AGG | 0.596177 | 4.2:+90406967 | None:intergenic |
| GGAAGAGTTCTGTTGTGAAG+AGG | 0.606689 | 4.2:-90407095 | MS.gene61226:CDS |
| AAACTATCAAGAGCAAGAGC+AGG | 0.606845 | 4.2:+90407000 | None:intergenic |
| CTGCTGTTGAAGATATCAGG+TGG | 0.617099 | 4.2:-90406897 | MS.gene61226:CDS |
| GGAGATGATCATGATAGCAA+TGG | 0.631701 | 4.2:-90406931 | MS.gene61226:CDS |
| GCTCATTGTGTGCAAAAGTG+AGG | 0.635769 | 4.2:+90407406 | None:intergenic |
| TGCTGTTGAAGATATCAGGT+GGG | 0.636800 | 4.2:-90406896 | MS.gene61226:CDS |
| AAGCCAAGAGAACCAATCCA+TGG | 0.643677 | 4.2:+90407470 | None:intergenic |
| ATTATGGAACAAGATCATCA+TGG | 0.658028 | 4.2:-90407116 | MS.gene61226:CDS |
| TGAGCTAGAACAAGAAGACA+TGG | 0.668504 | 4.2:-90407388 | MS.gene61226:CDS |
| GAATCTCCATAGCCAAACAC+AGG | 0.676222 | 4.2:-90407332 | MS.gene61226:CDS |
| TGTTGTAGTTCCAACAACAA+GGG | 0.688058 | 4.2:-90407154 | MS.gene61226:CDS |
| TGAAGGTGGATAAACAAGAG+AGG | 0.697861 | 4.2:-90407062 | MS.gene61226:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACTTGCAAGAAAAGAAAA+AGG | - | chr4.2:90407035-90407054 | MS.gene61226:CDS | 25.0% |
| ! | TAACTACATAATGAGAAAGT+TGG | - | chr4.2:90407127-90407146 | MS.gene61226:CDS | 25.0% |
| ! | TCATGAAAATGATAAACCAT+AGG | + | chr4.2:90407368-90407387 | None:intergenic | 25.0% |
| !! | ATGGTTTATCATTTTCATGA+AGG | - | chr4.2:90407368-90407387 | MS.gene61226:CDS | 25.0% |
| !!! | AAAAAGACAAAAAAGTGCTT+AGG | - | chr4.2:90407104-90407123 | MS.gene61226:CDS | 25.0% |
| ATCATGATAGCAATGGTTAT+TGG | - | chr4.2:90407408-90407427 | MS.gene61226:CDS | 30.0% | |
| ATTATGGAACAAGATCATCA+TGG | - | chr4.2:90407216-90407235 | MS.gene61226:CDS | 30.0% | |
| GCTAGTAGTAGTAGTTATTA+TGG | - | chr4.2:90407200-90407219 | MS.gene61226:CDS | 30.0% | |
| TTTCATGAAGGAAAGATGAA+AGG | - | chr4.2:90407380-90407399 | MS.gene61226:CDS | 30.0% | |
| ! | TAGTTTTTGTAGTACTCCTA+TGG | - | chr4.2:90407349-90407368 | MS.gene61226:CDS | 30.0% |
| CATATACAAGAGATGGATCA+AGG | + | chr4.2:90406899-90406918 | None:intergenic | 35.0% | |
| CATGAATTTCTCCAAAGCAT+GGG | - | chr4.2:90406978-90406997 | MS.gene61226:CDS | 35.0% | |
| CCATACTCATATACAAGAGA+TGG | + | chr4.2:90406906-90406925 | None:intergenic | 35.0% | |
| CCATCTCTTGTATATGAGTA+TGG | - | chr4.2:90406903-90406922 | MS.gene61226:CDS | 35.0% | |
| GAAGAGGAATAAGAAGTTGA+AGG | - | chr4.2:90407253-90407272 | MS.gene61226:CDS | 35.0% | |
| TGTTGTAGTTCCAACAACAA+GGG | - | chr4.2:90407178-90407197 | MS.gene61226:CDS | 35.0% | |
| TTGTTGTAGTTCCAACAACA+AGG | - | chr4.2:90407177-90407196 | MS.gene61226:CDS | 35.0% | |
| AAACTATCAAGAGCAAGAGC+AGG | + | chr4.2:90407335-90407354 | None:intergenic | 40.0% | |
| AAGACCAATGGCAAAGATCA+TGG | - | chr4.2:90407073-90407092 | MS.gene61226:CDS | 40.0% | |
| AGACCAATGGCAAAGATCAT+GGG | - | chr4.2:90407074-90407093 | MS.gene61226:CDS | 40.0% | |
| CCATGAATTTCTCCAAAGCA+TGG | - | chr4.2:90406977-90406996 | MS.gene61226:CDS | 40.0% | |
| GAGGAATAAGAAGTTGAAGG+TGG | - | chr4.2:90407256-90407275 | MS.gene61226:CDS | 40.0% | |
| GGAGATGATCATGATAGCAA+TGG | - | chr4.2:90407401-90407420 | MS.gene61226:CDS | 40.0% | |
| TGAAGGTGGATAAACAAGAG+AGG | - | chr4.2:90407270-90407289 | MS.gene61226:CDS | 40.0% | |
| TGAGCTAGAACAAGAAGACA+TGG | - | chr4.2:90406944-90406963 | MS.gene61226:CDS | 40.0% | |
| TGCTGTTGAAGATATCAGGT+GGG | - | chr4.2:90407436-90407455 | MS.gene61226:CDS | 40.0% | |
| ! | ATGATCTTTGCCATTGGTCT+TGG | + | chr4.2:90407074-90407093 | None:intergenic | 40.0% |
| !! | CCATGCTTTGGAGAAATTCA+TGG | + | chr4.2:90406980-90406999 | None:intergenic | 40.0% |
| !! | GAGAATTTCTAGCCTGTGTT+TGG | + | chr4.2:90407015-90407034 | None:intergenic | 40.0% |
| CAGCTGCTGTTGAAGATATC+AGG | - | chr4.2:90407432-90407451 | MS.gene61226:CDS | 45.0% | |
| CAGGCATCATTGAAGGACTA+GGG | + | chr4.2:90407316-90407335 | None:intergenic | 45.0% | |
| CTATGGAGATTCCCATGCTT+TGG | + | chr4.2:90406992-90407011 | None:intergenic | 45.0% | |
| CTGCTGTTGAAGATATCAGG+TGG | - | chr4.2:90407435-90407454 | MS.gene61226:CDS | 45.0% | |
| GAATCTCCATAGCCAAACAC+AGG | - | chr4.2:90407000-90407019 | MS.gene61226:CDS | 45.0% | |
| GCTCATTGTGTGCAAAAGTG+AGG | + | chr4.2:90406929-90406948 | None:intergenic | 45.0% | |
| GGAAGAGTTCTGTTGTGAAG+AGG | - | chr4.2:90407237-90407256 | MS.gene61226:CDS | 45.0% | |
| TTCTAGCCTGTGTTTGGCTA+TGG | + | chr4.2:90407009-90407028 | None:intergenic | 45.0% | |
| ! | CTACTACTAGCCCTTGTTGT+TGG | + | chr4.2:90407191-90407210 | None:intergenic | 45.0% |
| ! | GAGAGGCTGTTGCTTACAAA+TGG | - | chr4.2:90407287-90407306 | MS.gene61226:CDS | 45.0% |
| !! | ATCTTTGCCATTGGTCTTGG+TGG | + | chr4.2:90407071-90407090 | None:intergenic | 45.0% |
| GAGACAACCACCAAGACCAA+TGG | - | chr4.2:90407061-90407080 | MS.gene61226:CDS | 50.0% | |
| GCAAGAGCAGGCATCATTGA+AGG | + | chr4.2:90407323-90407342 | None:intergenic | 50.0% | |
| GCAGGCATCATTGAAGGACT+AGG | + | chr4.2:90407317-90407336 | None:intergenic | 50.0% | |
| GCTCCCATGATCTTTGCCAT+TGG | + | chr4.2:90407080-90407099 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 90406864 | 90407490 | 90406864 | ID=MS.gene61226 |
| chr4.2 | mRNA | 90406864 | 90407490 | 90406864 | ID=MS.gene61226.t1;Parent=MS.gene61226 |
| chr4.2 | exon | 90406864 | 90407490 | 90406864 | ID=MS.gene61226.t1.exon1;Parent=MS.gene61226.t1 |
| chr4.2 | CDS | 90406864 | 90407490 | 90406864 | ID=cds.MS.gene61226.t1;Parent=MS.gene61226.t1 |
| Gene Sequence |
| Protein sequence |
