AlfalfaGEDB | MS.gene62895

M. sativa cultivar XinJiangDaYe / MS.gene62895

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62895.t1	XP_003608722.2	62.3	236	3	2	1	150	1	236	2.20E-56	228.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62895.t1	G7JIX6	62.3	236	3	2	1	150	1	236	1.6e-56	228.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04919	MS.gene62895	0.810018	1.42E-50	-1.69E-46
MS.gene04920	MS.gene62895	0.820482	6.70E-53	-1.69E-46
MS.gene049283	MS.gene62895	0.814561	1.45E-51	-1.69E-46
MS.gene049419	MS.gene62895	-0.843828	1.10E-58	-1.69E-46
MS.gene049499	MS.gene62895	0.835056	2.10E-56	-1.69E-46
MS.gene049983	MS.gene62895	0.825748	3.96E-54	-1.69E-46
MS.gene050654	MS.gene62895	0.806691	7.27E-50	-1.69E-46
MS.gene050737	MS.gene62895	0.809901	1.50E-50	-1.69E-46
MS.gene050941	MS.gene62895	0.837594	4.74E-57	-1.69E-46
MS.gene051275	MS.gene62895	0.808225	3.44E-50	-1.69E-46
MS.gene051831	MS.gene62895	0.8187	1.71E-52	-1.69E-46
MS.gene052242	MS.gene62895	-0.81125	7.69E-51	-1.69E-46
MS.gene052493	MS.gene62895	0.819378	1.20E-52	-1.69E-46
MS.gene052674	MS.gene62895	0.817757	2.80E-52	-1.69E-46
MS.gene052675	MS.gene62895	0.81621	6.22E-52	-1.69E-46
MS.gene052677	MS.gene62895	0.812537	4.03E-51	-1.69E-46
MS.gene053006	MS.gene62895	0.809382	1.95E-50	-1.69E-46
MS.gene053726	MS.gene62895	0.83304	6.70E-56	-1.69E-46
MS.gene053963	MS.gene62895	0.817151	3.83E-52	-1.69E-46
MS.gene054479	MS.gene62895	0.84412	9.20E-59	-1.69E-46
MS.gene054892	MS.gene62895	0.835839	1.33E-56	-1.69E-46
MS.gene054893	MS.gene62895	0.838298	3.12E-57	-1.69E-46
MS.gene055724	MS.gene62895	0.812264	4.63E-51	-1.69E-46
MS.gene056174	MS.gene62895	0.800927	1.15E-48	-1.69E-46
MS.gene056699	MS.gene62895	0.825189	5.37E-54	-1.69E-46
MS.gene056776	MS.gene62895	0.809203	2.12E-50	-1.69E-46
MS.gene056779	MS.gene62895	0.809776	1.60E-50	-1.69E-46
MS.gene056787	MS.gene62895	0.810427	1.16E-50	-1.69E-46
MS.gene057155	MS.gene62895	0.853049	3.09E-61	-1.69E-46
MS.gene057208	MS.gene62895	0.80568	1.19E-49	-1.69E-46
MS.gene058429	MS.gene62895	0.835793	1.36E-56	-1.69E-46
MS.gene058592	MS.gene62895	0.8045	2.10E-49	-1.69E-46
MS.gene05878	MS.gene62895	0.80093	1.14E-48	-1.69E-46
MS.gene059283	MS.gene62895	0.807509	4.88E-50	-1.69E-46
MS.gene05952	MS.gene62895	0.839055	1.99E-57	-1.69E-46
MS.gene059952	MS.gene62895	0.80486	1.76E-49	-1.69E-46
MS.gene060259	MS.gene62895	0.847058	1.47E-59	-1.69E-46
MS.gene060412	MS.gene62895	0.810661	1.03E-50	-1.69E-46
MS.gene060520	MS.gene62895	0.820101	8.19E-53	-1.69E-46
MS.gene060840	MS.gene62895	0.801779	7.66E-49	-1.69E-46
MS.gene060850	MS.gene62895	0.845683	3.48E-59	-1.69E-46
MS.gene060851	MS.gene62895	0.848998	4.29E-60	-1.69E-46
MS.gene060852	MS.gene62895	0.849766	2.62E-60	-1.69E-46
MS.gene060932	MS.gene62895	0.839325	1.70E-57	-1.69E-46
MS.gene060933	MS.gene62895	0.805513	1.29E-49	-1.69E-46
MS.gene060937	MS.gene62895	0.809335	1.99E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62895.t1	MTR_4g101030	80.368	163	0	1	1	131	1	163	3.43e-84	247

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62895.t1	AT4G16535	47.449	196	45	4	1	143	1	191	3.86e-37	127

Find 26 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGTTTAAGCTGTTCTTCTT+TGG	0.295938	8.1:-22260722	None:intergenic
AATTTATATTTCTCATCTTC+CGG	0.307288	8.1:-22261048	None:intergenic
GTTATGGTGTTGCTGGGAAA+GGG	0.314214	8.1:+22261073	MS.gene62895:CDS
GAAGATGAGAAATATAAATT+AGG	0.320007	8.1:+22261051	MS.gene62895:CDS
AAATTAGGTTATGGTGTTGC+TGG	0.350657	8.1:+22261066	MS.gene62895:CDS
GAGAAATATAAATTAGGTTA+TGG	0.398827	8.1:+22261057	MS.gene62895:CDS
GGTTATGGTGTTGCTGGGAA+AGG	0.406889	8.1:+22261072	MS.gene62895:CDS
GGTGAGACTGTTCGTGCTGT+TGG	0.427915	8.1:+22261024	MS.gene62895:CDS
CGGCGGCTTCTTCGTCTTTG+CGG	0.430480	8.1:-22260699	None:intergenic
TCTCGACGACTTTGTTCCTT+CGG	0.442321	8.1:-22261288	None:intergenic
TTATGGTGTTGCTGGGAAAG+GGG	0.455179	8.1:+22261074	MS.gene62895:CDS
GAACAACTCCGTGTTGCTAA+AGG	0.455180	8.1:+22260781	MS.gene62895:CDS
GGTCATATTAATTTATTCAA+AGG	0.480959	8.1:+22260898	MS.gene62895:intron
AATTAGGTTATGGTGTTGCT+GGG	0.492710	8.1:+22261067	MS.gene62895:CDS
GGAAAGGGGTTGAATTGCCT+TGG	0.497813	8.1:+22261088	MS.gene62895:CDS
CGCGTTGAGGCGGCATCCGA+AGG	0.500359	8.1:+22261272	MS.gene62895:intron
ATTATAAGAAGTTGAAGATG+AGG	0.510408	8.1:+22260992	MS.gene62895:CDS
GACTGTTCGTGCTGTTGGAC+CGG	0.511227	8.1:+22261029	MS.gene62895:CDS
TTTAAGCTGTTCTTCTTTGG+CGG	0.512755	8.1:-22260719	None:intergenic
GAGTTGTTCAAGACGGACTT+CGG	0.535233	8.1:-22260767	None:intergenic
AAGCTGTTCTTCTTTGGCGG+CGG	0.548709	8.1:-22260716	None:intergenic
TTGAAGATGAGGAAAGAGCC+TGG	0.554510	8.1:+22261003	MS.gene62895:CDS
TTGCAAGTGATAGATACTAC+AGG	0.596940	8.1:+22261316	MS.gene62895:CDS
GTGCCAAACCTTTAGCAACA+CGG	0.641531	8.1:-22260789	None:intergenic
ACAGCACGAACAGTCTCACC+AGG	0.646246	8.1:-22261021	None:intergenic
CAACACGGAGTTGTTCAAGA+CGG	0.674484	8.1:-22260774	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTATATTTCTCATCTTC+CGG	-	chr8.1:22261051-22261070	None:intergenic	20.0%
!!	GAAGATGAGAAATATAAATT+AGG	+	chr8.1:22261051-22261070	MS.gene62895:CDS	20.0%
!!	GAGAAATATAAATTAGGTTA+TGG	+	chr8.1:22261057-22261076	MS.gene62895:CDS	20.0%
!!	GGTCATATTAATTTATTCAA+AGG	+	chr8.1:22260898-22260917	MS.gene62895:intron	20.0%
!	ATTATAAGAAGTTGAAGATG+AGG	+	chr8.1:22260992-22261011	MS.gene62895:CDS	25.0%
!	AGAGATTGTTGATGAAAAAG+AGG	+	chr8.1:22260957-22260976	MS.gene62895:CDS	30.0%
!	TCATCAACAATCTCTCTTTT+CGG	-	chr8.1:22260952-22260971	None:intergenic	30.0%
!	TTCAATCATGATGATGATGT+TGG	+	chr8.1:22261129-22261148	MS.gene62895:intron	30.0%
!	TTCTTCTTTTGAACATACCA+AGG	-	chr8.1:22261108-22261127	None:intergenic	30.0%
	TGAGAAAAGGAAGAAGACAT+TGG	+	chr8.1:22261194-22261213	MS.gene62895:intron	35.0%
	TTGCAAGTGATAGATACTAC+AGG	+	chr8.1:22261316-22261335	MS.gene62895:CDS	35.0%
	TTTAAGCTGTTCTTCTTTGG+CGG	-	chr8.1:22260722-22260741	None:intergenic	35.0%
!	AAACGTTCCAACGTTTTTGA+GGG	-	chr8.1:22260654-22260673	None:intergenic	35.0%
!	AATCATGATGATGATGTTGG+TGG	+	chr8.1:22261132-22261151	MS.gene62895:intron	35.0%
!	TAAACGTTCCAACGTTTTTG+AGG	-	chr8.1:22260655-22260674	None:intergenic	35.0%
!!	AAATTAGGTTATGGTGTTGC+TGG	+	chr8.1:22261066-22261085	MS.gene62895:CDS	35.0%
!!	AATTAGGTTATGGTGTTGCT+GGG	+	chr8.1:22261067-22261086	MS.gene62895:CDS	35.0%
	ACATTCTCCCTCAAAAACGT+TGG	+	chr8.1:22260644-22260663	MS.gene62895:CDS	40.0%
	AGAAGACATTGGAAGAGTTG+AGG	+	chr8.1:22261205-22261224	MS.gene62895:intron	40.0%
	GAAAAGGGAGAAGAAAGAGA+AGG	+	chr8.1:22261239-22261258	MS.gene62895:intron	40.0%
	GAAGACATTGGAAGAGTTGA+GGG	+	chr8.1:22261206-22261225	MS.gene62895:intron	40.0%
	GCGTTTAAGCTGTTCTTCTT+TGG	-	chr8.1:22260725-22260744	None:intergenic	40.0%
!	TCTCTTTTCGGTACACGAAT+CGG	-	chr8.1:22260940-22260959	None:intergenic	40.0%
!	TTCAGCTTCGGATTTAGGTT+TGG	-	chr8.1:22260848-22260867	None:intergenic	40.0%
!	TTTAGGTTTGGCTTCAGGTT+CGG	-	chr8.1:22260836-22260855	None:intergenic	40.0%
!!	TTCGATTTCAGGTTCAGCTT+CGG	-	chr8.1:22260860-22260879	None:intergenic	40.0%
	CAACACGGAGTTGTTCAAGA+CGG	-	chr8.1:22260777-22260796	None:intergenic	45.0%
	CCTGAAATCGAAGACAAAGC+TGG	+	chr8.1:22260868-22260887	MS.gene62895:intron	45.0%
	GAGGGAAGAGAGGTTGAAAA+GGG	+	chr8.1:22261224-22261243	MS.gene62895:intron	45.0%
	GGAGAAGAAAGAGAAGGAGA+GGG	+	chr8.1:22261245-22261264	MS.gene62895:intron	45.0%
	GTGATAGGAAGGGTGAGAAA+AGG	+	chr8.1:22261181-22261200	MS.gene62895:intron	45.0%
	GTGCCAAACCTTTAGCAACA+CGG	-	chr8.1:22260792-22260811	None:intergenic	45.0%
	TCAGGTTCAGCTTCGGATTT+AGG	-	chr8.1:22260853-22260872	None:intergenic	45.0%
	TCTCGACGACTTTGTTCCTT+CGG	-	chr8.1:22261291-22261310	None:intergenic	45.0%
	TGAGGGAAGAGAGGTTGAAA+AGG	+	chr8.1:22261223-22261242	MS.gene62895:intron	45.0%
	TTGAAGATGAGGAAAGAGCC+TGG	+	chr8.1:22261003-22261022	MS.gene62895:CDS	45.0%
!	ACTCCGTGTTGCTAAAGGTT+TGG	+	chr8.1:22260786-22260805	MS.gene62895:intron	45.0%
!	CATGATGATGATGTTGGTGG+TGG	+	chr8.1:22261135-22261154	MS.gene62895:intron	45.0%
!	CCAGCTTTGTCTTCGATTTC+AGG	-	chr8.1:22260871-22260890	None:intergenic	45.0%
!	GAACAACTCCGTGTTGCTAA+AGG	+	chr8.1:22260781-22260800	MS.gene62895:CDS	45.0%
!	GAAGACAAAGCTGGTGATGT+AGG	+	chr8.1:22260877-22260896	MS.gene62895:intron	45.0%
!	GAGTTGTTCAAGACGGACTT+CGG	-	chr8.1:22260770-22260789	None:intergenic	45.0%
!	TCGGATTTAGGTTTGGCTTC+AGG	-	chr8.1:22260841-22260860	None:intergenic	45.0%
!!	AAATGGTGGTGGTGATAGGA+AGG	+	chr8.1:22261170-22261189	MS.gene62895:intron	45.0%
!!	AATGGTGGTGGTGATAGGAA+GGG	+	chr8.1:22261171-22261190	MS.gene62895:intron	45.0%
!!	GTTATGGTGTTGCTGGGAAA+GGG	+	chr8.1:22261073-22261092	MS.gene62895:CDS	45.0%
!!	TTATGGTGTTGCTGGGAAAG+GGG	+	chr8.1:22261074-22261093	MS.gene62895:CDS	45.0%
	AAGCTGTTCTTCTTTGGCGG+CGG	-	chr8.1:22260719-22260738	None:intergenic	50.0%
	GGAAAGGGGTTGAATTGCCT+TGG	+	chr8.1:22261088-22261107	MS.gene62895:CDS	50.0%
	GGGAGAAGAAAGAGAAGGAG+AGG	+	chr8.1:22261244-22261263	MS.gene62895:intron	50.0%
	TGGAAGAGTTGAGGGAAGAG+AGG	+	chr8.1:22261214-22261233	MS.gene62895:intron	50.0%
!	TTGGCTTCAGGTTCGGATTC+AGG	-	chr8.1:22260829-22260848	None:intergenic	50.0%
!!	GATGATAGCGGAAATGGTGG+TGG	+	chr8.1:22261159-22261178	MS.gene62895:intron	50.0%
!!	GATTCAGGTGGCGGAATTGT+TGG	-	chr8.1:22260814-22260833	None:intergenic	50.0%
!!	GGTGATGATAGCGGAAATGG+TGG	+	chr8.1:22261156-22261175	MS.gene62895:intron	50.0%
!!	GGTGGTGATGATAGCGGAAA+TGG	+	chr8.1:22261153-22261172	MS.gene62895:intron	50.0%
!!	GGTTATGGTGTTGCTGGGAA+AGG	+	chr8.1:22261072-22261091	MS.gene62895:CDS	50.0%
!!	GTTGGTGGTGGTGATGATAG+CGG	+	chr8.1:22261147-22261166	MS.gene62895:intron	50.0%
	ACAGCACGAACAGTCTCACC+AGG	-	chr8.1:22261024-22261043	None:intergenic	55.0%
	GGTGAGACTGTTCGTGCTGT+TGG	+	chr8.1:22261024-22261043	MS.gene62895:CDS	55.0%
	TCAGGTTCGGATTCAGGTGG+CGG	-	chr8.1:22260823-22260842	None:intergenic	55.0%
!	GACTGTTCGTGCTGTTGGAC+CGG	+	chr8.1:22261029-22261048	MS.gene62895:CDS	55.0%
!	GCTTCAGGTTCGGATTCAGG+TGG	-	chr8.1:22260826-22260845	None:intergenic	55.0%
!!	GCGGAAATGGTGGTGGTGAT+AGG	+	chr8.1:22261166-22261185	MS.gene62895:intron	55.0%
	CGGCGGCTTCTTCGTCTTTG+CGG	-	chr8.1:22260702-22260721	None:intergenic	60.0%
!	AGAGGGAGAGCGCGTTGAGG+CGG	+	chr8.1:22261262-22261281	MS.gene62895:intron	65.0%
!	AGGAGAGGGAGAGCGCGTTG+AGG	+	chr8.1:22261259-22261278	MS.gene62895:intron	65.0%
	CGCGTTGAGGCGGCATCCGA+AGG	+	chr8.1:22261272-22261291	MS.gene62895:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	22260622	22261341	22260622	ID=MS.gene62895
chr8.1	mRNA	22260622	22261341	22260622	ID=MS.gene62895.t1;Parent=MS.gene62895
chr8.1	exon	22260622	22260802	22260622	ID=MS.gene62895.t1.exon1;Parent=MS.gene62895.t1
chr8.1	CDS	22260622	22260802	22260622	ID=cds.MS.gene62895.t1;Parent=MS.gene62895.t1
chr8.1	exon	22260899	22261109	22260899	ID=MS.gene62895.t1.exon2;Parent=MS.gene62895.t1
chr8.1	CDS	22260899	22261109	22260899	ID=cds.MS.gene62895.t1;Parent=MS.gene62895.t1
chr8.1	exon	22261281	22261341	22261281	ID=MS.gene62895.t1.exon3;Parent=MS.gene62895.t1
chr8.1	CDS	22261281	22261341	22261281	ID=cds.MS.gene62895.t1;Parent=MS.gene62895.t1

Gene Sequence

>MS.gene62895

ATGGGTGGACATGGCGGTTTGAACATTCTCCCTCAAAAACGTTGGAACGTTTACAATTTCGATAACAGAGAAAAAGTCCGCAAAGACGAAGAAGCCGCCGCCAAAGAAGAACAGCTTAAACGCGAACAATCGCGAAAACGCGACACCGAAGTCCGTCTTGAACAACTCCGTGTTGCTAAAGGTTTGGCACCAACAATTCCGCCACCTGAATCCGAACCTGAAGCCAAACCTAAATCCGAAGCTGAACCTGAAATCGAAGACAAAGCTGGTGATGTAGGTCATATTAATTTATTCAAAGGAATTAAGATTTTTGATCCGATTCGTGTACCGAAAAGAGAGATTGTTGATGAAAAAGAGGAATTGAAAAAGAATTATAAGAAGTTGAAGATGAGGAAAGAGCCTGGTGAGACTGTTCGTGCTGTTGGACCGGAAGATGAGAAATATAAATTAGGTTATGGTGTTGCTGGGAAAGGGGTTGAATTGCCTTGGTATGTTCAAAAGAAGAAATTCAATCATGATGATGATGTTGGTGGTGGTGATGATAGCGGAAATGGTGGTGGTGATAGGAAGGGTGAGAAAAGGAAGAAGACATTGGAAGAGTTGAGGGAAGAGAGGTTGAAAAGGGAGAAGAAAGAGAAGGAGAGGGAGAGCGCGTTGAGGCGGCATCCGAAGGAACAAAGTCGTCGAGATGCTGTTGCAAGTGATAGATACTACAGGTGA

Protein sequence

>MS.gene62895.t1

MGGHGGLNILPQKRWNVYNFDNREKVRKDEEAAAKEEQLKREQSRKRDTEVRLEQLRVAKGHINLFKGIKIFDPIRVPKREIVDEKEELKKNYKKLKMRKEPGETVRAVGPEDEKYKLGYGVAGKGVELPWRHPKEQSRRDAVASDRYYR