AlfalfaGEDB | MS.gene64466

M. sativa cultivar XinJiangDaYe / MS.gene64466

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64466.t1	XP_003595315.1	92.5	53	4	0	1	53	81	133	6.30E-19	103.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64466.t1	G7IGG4	92.5	53	4	0	1	53	81	133	4.5e-19	103.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene64466	0.801671	8.07E-49	-1.69E-46
MS.gene049153	MS.gene64466	0.811382	7.20E-51	-1.69E-46
MS.gene049356	MS.gene64466	0.800856	1.18E-48	-1.69E-46
MS.gene050060	MS.gene64466	0.828673	7.89E-55	-1.69E-46
MS.gene050128	MS.gene64466	0.814509	1.49E-51	-1.69E-46
MS.gene050301	MS.gene64466	0.801824	7.50E-49	-1.69E-46
MS.gene050426	MS.gene64466	-0.801214	1.00E-48	-1.69E-46
MS.gene052077	MS.gene64466	0.821745	3.43E-53	-1.69E-46
MS.gene052188	MS.gene64466	0.848407	6.25E-60	-1.69E-46
MS.gene052253	MS.gene64466	0.817233	3.67E-52	-1.69E-46
MS.gene052340	MS.gene64466	0.849624	2.87E-60	-1.69E-46
MS.gene05316	MS.gene64466	0.85751	1.55E-62	-1.69E-46
MS.gene053557	MS.gene64466	0.805215	1.49E-49	-1.69E-46
MS.gene053928	MS.gene64466	0.87087	1.05E-66	-1.69E-46
MS.gene054123	MS.gene64466	0.826622	2.45E-54	-1.69E-46
MS.gene054183	MS.gene64466	0.823194	1.58E-53	-1.69E-46
MS.gene054349	MS.gene64466	0.869623	2.70E-66	-1.69E-46
MS.gene055539	MS.gene64466	0.816779	4.64E-52	-1.69E-46
MS.gene05553	MS.gene64466	0.870374	1.53E-66	-1.69E-46
MS.gene055604	MS.gene64466	0.804768	1.84E-49	-1.69E-46
MS.gene056659	MS.gene64466	0.806945	6.43E-50	-1.69E-46
MS.gene056723	MS.gene64466	0.836936	6.99E-57	-1.69E-46
MS.gene057124	MS.gene64466	0.804202	2.42E-49	-1.69E-46
MS.gene057396	MS.gene64466	0.919226	5.87E-87	-1.69E-46
MS.gene057749	MS.gene64466	0.802778	4.77E-49	-1.69E-46
MS.gene057825	MS.gene64466	0.812484	4.14E-51	-1.69E-46
MS.gene058002	MS.gene64466	0.806338	8.64E-50	-1.69E-46
MS.gene058007	MS.gene64466	0.808615	2.84E-50	-1.69E-46
MS.gene058142	MS.gene64466	0.835116	2.02E-56	-1.69E-46
MS.gene058365	MS.gene64466	0.820518	6.58E-53	-1.69E-46
MS.gene058928	MS.gene64466	0.813115	3.01E-51	-1.69E-46
MS.gene058949	MS.gene64466	0.821841	3.26E-53	-1.69E-46
MS.gene059011	MS.gene64466	0.852057	5.93E-61	-1.69E-46
MS.gene05907	MS.gene64466	0.803185	3.93E-49	-1.69E-46
MS.gene059234	MS.gene64466	0.816098	6.59E-52	-1.69E-46
MS.gene059420	MS.gene64466	0.830038	3.68E-55	-1.69E-46
MS.gene059788	MS.gene64466	0.819636	1.05E-52	-1.69E-46
MS.gene059894	MS.gene64466	0.801907	7.21E-49	-1.69E-46
MS.gene060247	MS.gene64466	0.8237	1.20E-53	-1.69E-46
MS.gene060407	MS.gene64466	0.828353	9.42E-55	-1.69E-46
MS.gene060495	MS.gene64466	0.821489	3.93E-53	-1.69E-46
MS.gene060496	MS.gene64466	0.80451	2.09E-49	-1.69E-46
MS.gene061049	MS.gene64466	0.826096	3.27E-54	-1.69E-46
MS.gene061159	MS.gene64466	0.813864	2.06E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64466.t1	MTR_2g042420	92.453	53	4	0	1	53	81	133	1.06e-27	102
MS.gene64466.t1	MTR_2g042370	90.741	54	4	1	1	53	88	141	5.60e-26	98.6
MS.gene64466.t1	MTR_2g042430	86.792	53	7	0	1	53	100	152	3.07e-25	96.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATGGTTAGAAATGGAAAT+TGG	0.206093	2.4:-47914895	MS.gene64466:CDS
GAAATGGAAATTGGGAGAAT+TGG	0.304056	2.4:-47914886	MS.gene64466:CDS
ATTAAAAGAATGGTTAGAAA+TGG	0.392540	2.4:-47914902	None:intergenic
AATGGTTAGAAATGGAAATT+GGG	0.411141	2.4:-47914894	MS.gene64466:CDS
CTTGCGGCCGCGTCATATGA+TGG	0.411626	2.4:+47914355	None:intergenic
AATTGCGGCTGCATGCAGTT+GGG	0.413898	2.4:+47914322	None:intergenic
TTGCTCAAGGTTACAGAAGA+AGG	0.424204	2.4:-47914550	MS.gene64466:CDS
CAATTGCGGCTGCATGCAGT+TGG	0.446137	2.4:+47914321	None:intergenic
ATTGCGGCTGCATGCAGTTG+GGG	0.529158	2.4:+47914323	None:intergenic
GAGAACCATTCACTTGTGAA+AGG	0.565917	2.4:+47914515	None:intergenic
CATTGTCTGTGATACTACCT+TGG	0.569110	2.4:-47914807	MS.gene64466:intron
GGAATCACTACATCTAAAGA+TGG	0.570283	2.4:-47914836	MS.gene64466:CDS
GTTGGGGACACTGCTGCTTG+CGG	0.637477	2.4:+47914339	None:intergenic
ATCACTACATCTAAAGATGG+TGG	0.642182	2.4:-47914833	MS.gene64466:CDS
GAAATGTTGTGAGGCAATTG+CGG	0.653338	2.4:+47914307	None:intergenic
TACTACAGTGAAATGTTGTG+AGG	0.653799	2.4:+47914298	None:intergenic
TTGTAGACCATCATATGACG+CGG	0.732559	2.4:-47914362	MS.gene64466:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATATAATTCATGTAAATT+TGG	-	chr2.4:47914469-47914488	MS.gene64466:intron	10.0%
!!!	GAATTTTCTTAAGAATATTT+AGG	-	chr2.4:47914553-47914572	MS.gene64466:CDS	15.0%
!	CAAACATGAATTATTTACCA+AGG	+	chr2.4:47914406-47914425	None:intergenic	25.0%
!	CTTGGTAAATAATTCATGTT+TGG	-	chr2.4:47914404-47914423	MS.gene64466:intron	25.0%
!!	AATATATACCATGCTTTTCT+TGG	+	chr2.4:47914515-47914534	None:intergenic	25.0%
!!	AATATTTAGGAGCCAATTTT+AGG	-	chr2.4:47914566-47914585	MS.gene64466:intron	25.0%
!!!	TTGTTTTGTTCTTTTGTTTG+TGG	-	chr2.4:47914752-47914771	MS.gene64466:intron	25.0%
	AAGCATGGTATATATTCATG+TGG	-	chr2.4:47914519-47914538	MS.gene64466:intron	30.0%
	AGCATGGTATATATTCATGT+GGG	-	chr2.4:47914520-47914539	MS.gene64466:intron	30.0%
	ATCAATGCAGAATTGTATCT+TGG	+	chr2.4:47914593-47914612	None:intergenic	30.0%
	TTGTATCTTGGACCTAAAAT+TGG	+	chr2.4:47914581-47914600	None:intergenic	30.0%
!	AACACATAGTTACATTTTGC+AGG	-	chr2.4:47914618-47914637	MS.gene64466:intron	30.0%
!	ACACATAGTTACATTTTGCA+GGG	-	chr2.4:47914619-47914638	MS.gene64466:intron	30.0%
!!!	ATAGATAGCTCTTTTTTGCT+TGG	-	chr2.4:47914336-47914355	MS.gene64466:CDS	30.0%
	ATCACTACATCTAAAGATGG+TGG	-	chr2.4:47914360-47914379	MS.gene64466:CDS	35.0%
	GAAATGGAAATTGGGAGAAT+TGG	-	chr2.4:47914307-47914326	MS.gene64466:CDS	35.0%
	GGAATCACTACATCTAAAGA+TGG	-	chr2.4:47914357-47914376	MS.gene64466:CDS	35.0%
	GTTATCCTTTCACAAGTGAA+TGG	-	chr2.4:47914673-47914692	MS.gene64466:intron	35.0%
!!	AGATTTTGAGTGTTACAAGC+AGG	-	chr2.4:47914800-47914819	MS.gene64466:intron	35.0%
!!	GATTTTGAGTGTTACAAGCA+GGG	-	chr2.4:47914801-47914820	MS.gene64466:intron	35.0%
	CATTGTCTGTGATACTACCT+TGG	-	chr2.4:47914386-47914405	MS.gene64466:CDS	40.0%
	GAAATGTTGTGAGGCAATTG+CGG	+	chr2.4:47914889-47914908	None:intergenic	40.0%
	GAGAACCATTCACTTGTGAA+AGG	+	chr2.4:47914681-47914700	None:intergenic	40.0%
	TTGCTCAAGGTTACAGAAGA+AGG	-	chr2.4:47914643-47914662	MS.gene64466:intron	40.0%
	TTGTAGACCATCATATGACG+CGG	-	chr2.4:47914831-47914850	MS.gene64466:CDS	40.0%
	GCAAGAAGCCAAGAAAAGCA+TGG	-	chr2.4:47914504-47914523	MS.gene64466:intron	45.0%
!!	CATTTTGCAGGGTTTGCTCA+AGG	-	chr2.4:47914630-47914649	MS.gene64466:intron	45.0%
	AATTGCGGCTGCATGCAGTT+GGG	+	chr2.4:47914874-47914893	None:intergenic	50.0%
	ATTGCGGCTGCATGCAGTTG+GGG	+	chr2.4:47914873-47914892	None:intergenic	55.0%
	CAATTGCGGCTGCATGCAGT+TGG	+	chr2.4:47914875-47914894	None:intergenic	55.0%
	CTTGCGGCCGCGTCATATGA+TGG	+	chr2.4:47914841-47914860	None:intergenic	60.0%
!	GTTGGGGACACTGCTGCTTG+CGG	+	chr2.4:47914857-47914876	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	47914300	47914915	47914300	ID=MS.gene64466
chr2.4	mRNA	47914300	47914915	47914300	ID=MS.gene64466.t1;Parent=MS.gene64466
chr2.4	exon	47914808	47914915	47914808	ID=MS.gene64466.t1.exon1;Parent=MS.gene64466.t1
chr2.4	CDS	47914808	47914915	47914808	ID=cds.MS.gene64466.t1;Parent=MS.gene64466.t1
chr2.4	exon	47914528	47914575	47914528	ID=MS.gene64466.t1.exon2;Parent=MS.gene64466.t1
chr2.4	CDS	47914528	47914575	47914528	ID=cds.MS.gene64466.t1;Parent=MS.gene64466.t1
chr2.4	exon	47914300	47914413	47914300	ID=MS.gene64466.t1.exon3;Parent=MS.gene64466.t1
chr2.4	CDS	47914300	47914413	47914300	ID=cds.MS.gene64466.t1;Parent=MS.gene64466.t1

Gene Sequence

>MS.gene64466

ATGGTTAGAAATGGAAATTGGGAGAATTGGAAGCATATAGATAGCTCTTTTTTGCTTGGAATCACTACATCTAAAGATGGTGGTCTCATTGTCTGTGATACTACCTTGGTAAATAATTCATGTTTGGATATAATTAATTTATTGAATTGCAAGAAACATATATATGTAAATATATAATTCATGTAAATTTGGACTTGTTGAATTGCAAGAAGCCAAGAAAAGCATGGTATATATTCATGTGGGACTTTGCTAAGAATTTTCTTAAGAATATTTAGGAGCCAATTTTAGGTCCAAGATACAATTCTGCATTGATCAAATAACACATAGTTACATTTTGCAGGGTTTGCTCAAGGTTACAGAAGAAGGTTTCAGTGTTATCCTTTCACAAGTGAATGGTTCTCAATTAATGTGAGTTTTAAACAAATAATTCATAAGAAGATTGATTTTCAGTTTTGTTTTGTTCTTTTGTTTGTGGCACTTGATATGTAATTTTTTTTTTAAGATTTTGAGTGTTACAAGCAGGGTTTCTAATTGTAGACCATCATATGACGCGGCCGCAAGCAGCAGTGTCCCCAACTGCATGCAGCCGCAATTGCCTCACAACATTTCACTGTAG

Protein sequence

>MS.gene64466.t1

MVRNGNWENWKHIDSSFLLGITTSKDGGLIVCDTTLGLLKVTEEGFSVILSQILSVTSRVSNCRPSYDAAASSSVPNCMQPQLPHNISL